FastQCFastQC Report
Fri 27 May 2016
SRR939910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682831
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32630.4778634830580334No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21820.3195519828478789No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16010.23446504332697254No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC10620.15552896690396306No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10220.1496710020488232No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9770.14308079158679088No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9220.13502608991097356No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8650.12667848999239928No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8590.12579979526412832No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8560.12536044789999282No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7620.11159423049041416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTT150.0018733472085.2246110-111
CTCCGCC850.01839.9042110-111
CAGTTCG150.00316696851604.6791108-109
GCCGGCA150.00316696851604.6791108-109
TCGCTCG150.00316696851604.6791108-109
CGGTGCG150.00316696851604.6791108-109
TCGCGTG150.00316696851604.6791108-109
AATCGCG150.00316696851604.6791108-109
GTGCGTT200.00333004371563.9185110-111
CAGCGCG551.8120772E-81312.9192108-109
CCGCTCC2500.01155.3689108-109
CCGCGCC2050.01056.7399108-109
GTTCGCG300.0074910181042.6123110-111
CGACGCA250.008794719962.8074108-109
CCGCGCT250.008794719962.8074108-109
CCGTTGC250.008794719962.8074108-109
AATCGCT250.0029357034962.8074108-109
CCGCACC1052.055458E-10916.9594108-109
CCTCACG951.6108788E-7760.1111108-109
GGTGTCG706.6192515E-5687.7196108-109
Produced by FastQC (version 0.11.5)