FastQCFastQC Report
Fri 27 May 2016
SRR939910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682831
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34200.5008559951144573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25590.374763301607572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23470.3437160878753308No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21980.32189516878993485No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15520.22728903637942627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12000.17573894565419557No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10670.15626121251085553No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC9690.1419091986157629No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9550.13985891091646396No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9450.138394419702679No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA9170.1342938443040811No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8430.12345660932207238No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8240.12067407601588094No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT8160.11950248304485296No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7060.10339307969321838No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7040.10310018145046139No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGC401.7618583E-459.5655023
ATATCCG750.003910762331.731059
GATGCGA750.003910762331.731056
GTACACG1152.7347902E-531.0890521
ACGCCCG603.6964884E-529.750036110-111
GAGTACT32400.029.5642312-13
GTACTTT34800.027.18338814-15
CGCCCGG557.75193E-427.045488110-111
AGTACTT34000.026.42309612-13
ATAGCCC3007.2759576E-1225.8117183
AGAGTAC52150.024.47139210-11
ACTTTTT41900.023.14511316-17
GTACATG29150.023.099041
ACATGGG28350.022.0612983
TACATGG30750.021.5016942
CATGGGG11400.021.4226824
TACTTTT42600.021.08885814-15
GTAATAC1703.9022713E-421.023123
GTAGGAC13350.020.5244453
GTCCTAC13000.020.1680261
Produced by FastQC (version 0.11.5)