FastQCFastQC Report
Fri 27 May 2016
SRR939909_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939909_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287327
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39041.3587306448750032No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20030.6971151336282354No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12420.4322601078214021No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11400.39676048543993425No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10720.37309407051895577No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7080.2464091435890118No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7000.2436248594806614No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC6930.24118861088585478No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA6920.24084057537231096No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG6680.23248772304725976No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA6660.23179165202017213No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC6390.22239469315448948No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA5810.20220863336894898No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC5290.18411078666467126No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA4380.15243955493218528No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4320.15035134185092247No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA3620.12598885590285633No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC3450.1200722521726117No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA3210.11171939984756045No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT3050.10615083163085962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG52.4137516E-45791.0117110-111
CGCCGCC52.4137516E-45791.0117110-111
CCGCTCG101.5697697E-75127.458108-109
CAGCTCG53.0830654E-45127.458108-109
TCGCGTG53.0830654E-45127.458108-109
CCGCTCC600.02991.017108-109
CTCCGAT109.653921E-42895.5059110-111
CGTTGGT109.653921E-42895.5059110-111
CTGCGCG109.653921E-42895.5059110-111
CTCGATG109.653921E-42895.5059110-111
CGCGTTG109.653921E-42895.5059110-111
CGCGTCT109.653921E-42895.5059110-111
CGGCGCG100.00123306912563.729108-109
CGACGCC100.00123306912563.729108-109
CCGCGCC401.8189894E-122563.729108-109
CCTCTCG352.222805E-92197.4822108-109
CTCCGCC453.7034624E-91930.3373110-111
CGTGTTG150.00217188821930.3372110-111
GTGCGCT150.00217188821930.3372110-111
CTGCTCG150.00277405261709.1527108-109
Produced by FastQC (version 0.11.5)