##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939909_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 287327 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.679508016998053 33.0 31.0 34.0 30.0 34.0 2 31.8814904272832 34.0 31.0 34.0 30.0 34.0 3 31.982455529762255 34.0 31.0 34.0 30.0 34.0 4 35.1706974979727 37.0 35.0 37.0 33.0 37.0 5 35.30224448102685 37.0 35.0 37.0 33.0 37.0 6 35.294298830252636 37.0 37.0 37.0 33.0 37.0 7 35.1957316924619 37.0 37.0 37.0 33.0 37.0 8 35.219244971756915 37.0 37.0 37.0 33.0 37.0 9 36.816418227315914 39.0 39.0 39.0 34.0 39.0 10-11 36.81661138702593 39.0 38.5 39.0 33.5 39.0 12-13 36.757873433405145 39.0 38.0 39.0 33.5 39.0 14-15 38.26491245166657 41.0 39.0 41.0 33.5 41.0 16-17 38.314622364066025 41.0 39.0 41.0 34.0 41.0 18-19 38.35612907941126 41.0 39.0 41.0 33.5 41.0 20-21 38.39266584762309 41.0 39.0 41.0 34.0 41.0 22-23 38.37976417113602 41.0 39.0 41.0 34.0 41.0 24-25 38.39427029134053 41.0 39.0 41.0 34.0 41.0 26-27 38.3439600176802 41.0 39.0 41.0 33.0 41.0 28-29 38.296277412147134 41.0 39.0 41.0 33.0 41.0 30-31 38.2073160545302 41.0 39.0 41.0 33.0 41.0 32-33 38.09321609177 40.0 38.0 41.0 33.0 41.0 34-35 37.99862003918879 40.0 38.0 41.0 33.0 41.0 36-37 38.162113202031136 41.0 39.0 41.0 33.0 41.0 38-39 38.09553922882291 41.0 38.5 41.0 33.0 41.0 40-41 38.02907662697902 41.0 38.5 41.0 33.0 41.0 42-43 37.96848016371591 41.0 38.0 41.0 33.0 41.0 44-45 37.87914466792192 41.0 38.0 41.0 32.5 41.0 46-47 37.738383444646686 40.0 38.0 41.0 32.0 41.0 48-49 37.622583328402825 40.0 38.0 41.0 32.0 41.0 50-51 37.4948734368855 40.0 38.0 41.0 31.0 41.0 52-53 37.42573792229759 40.0 38.0 41.0 31.0 41.0 54-55 37.26113278598948 40.0 38.0 41.0 30.5 41.0 56-57 36.98802235780139 40.0 37.0 41.0 30.0 41.0 58-59 36.804894771462486 40.0 37.0 41.0 30.0 41.0 60-61 36.549649006184595 40.0 36.0 41.0 30.0 41.0 62-63 36.36343782519569 40.0 36.0 41.0 30.0 41.0 64-65 36.08379825077351 39.0 35.5 41.0 29.0 41.0 66-67 35.71355807146561 39.0 35.0 41.0 28.5 41.0 68-69 35.22789017391335 38.5 35.0 40.5 28.0 41.0 70-71 34.73297149241108 37.5 34.0 40.0 27.0 41.0 72-73 34.33248354662109 37.0 34.0 39.5 26.5 41.0 74-75 33.92164328448075 36.5 34.0 39.0 26.0 41.0 76-77 33.42427443296314 36.0 34.0 39.0 26.0 40.5 78-79 32.869396193187555 35.5 33.0 38.5 24.5 40.0 80-81 32.31659572542782 35.0 33.0 37.0 21.0 39.0 82-83 32.05184337009749 35.0 33.0 37.0 20.5 39.0 84-85 31.54105252900006 35.0 33.0 36.5 7.0 39.0 86-87 30.818932783901268 35.0 33.0 36.0 2.0 37.0 88-89 15.41385250951007 18.0 16.0 18.5 2.0 19.5 90-91 2.733836360662242 2.0 2.0 2.0 2.0 2.0 92-93 2.2902320352768797 2.0 2.0 2.0 2.0 2.0 94-95 2.0969835762041162 2.0 2.0 2.0 2.0 2.0 96-97 2.0000904892335214 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.062303509044884464 1101 2 0.12187713383127985 1101 3 0.05791581301020088 1101 4 0.1640708369499393 1101 5 -0.015607577489788582 1101 6 0.14621690522495356 1101 7 -0.08652192055625818 1101 8 -0.03568661922532357 1101 9 0.14755170589992872 1101 10-11 0.036247725240116324 1101 12-13 -0.2081895665244815 1101 14-15 0.0829831142963684 1101 16-17 0.08501676425537141 1101 18-19 0.08970883955039 1101 20-21 -0.009562753981050776 1101 22-23 -0.07893114325167971 1101 24-25 -0.061125173216844075 1101 26-27 -0.04623534540657914 1101 28-29 -0.048101624070213234 1101 30-31 -0.14095299664901972 1101 32-33 0.026465388109343735 1101 34-35 0.0318870502418207 1101 36-37 -0.03943483802943604 1101 38-39 0.17316767589255733 1101 40-41 0.03001040247054476 1101 42-43 0.18590892032589323 1101 44-45 0.026135831065595028 1101 46-47 0.13270635672218845 1101 48-49 0.3380365529380498 1101 50-51 0.07531345535480227 1101 52-53 -0.15338937753009674 1101 54-55 0.07456940442657611 1101 56-57 0.06615689274293857 1101 58-59 -0.022606814370206507 1101 60-61 -0.04264358477851715 1101 62-63 -0.126487890886267 1101 64-65 -0.0963174721024842 1101 66-67 0.1386163945419625 1101 68-69 0.02012050467799753 1101 70-71 0.2864358937583873 1101 72-73 -0.36238552792475787 1101 74-75 -0.2798970084203134 1101 76-77 -0.09786959875900436 1101 78-79 -0.02847553590024887 1101 80-81 -0.13657952856181765 1101 82-83 -0.02390404733046836 1101 84-85 0.09244906898132754 1101 86-87 -0.022489912338965468 1101 88-89 -0.43802283560863486 1101 90-91 -0.31849304798991085 1101 92-93 -0.22628443646291618 1101 94-95 -0.05990733747488797 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.21404601381510702 1102 2 0.33527859611110955 1102 3 0.22997701037049723 1102 4 0.27906513975580793 1102 5 0.3614220479196959 1102 6 0.18971735625282093 1102 7 0.05783667033772133 1102 8 0.11038112990822668 1102 9 0.31805210945118034 1102 10-11 0.12339699172637353 1102 12-13 -0.10766079569411602 1102 14-15 0.10729707716964754 1102 16-17 0.101369491787068 1102 18-19 0.08863764836204524 1102 20-21 0.08812632164920586 1102 22-23 0.1649414057679266 1102 24-25 0.1013368454889374 1102 26-27 0.28738878221057007 1102 28-29 0.17829359503437558 1102 30-31 0.14104030914787558 1102 32-33 0.16276482313759288 1102 34-35 0.2939817186176441 1102 36-37 0.28569690958486404 1102 38-39 0.11746573409888583 1102 40-41 0.19927938124850186 1102 42-43 0.263444171714589 1102 44-45 0.0836804590863025 1102 46-47 0.25864105263445225 1102 48-49 0.38503543723753353 1102 50-51 0.13297974220853348 1102 52-53 -0.0569050210756572 1102 54-55 0.06886924300607689 1102 56-57 0.10847042832666887 1102 58-59 -0.0087436419826048 1102 60-61 0.17313467567820595 1102 62-63 -0.162685844644038 1102 64-65 1.2534231141358987E-4 1102 66-67 -0.10731248548461991 1102 68-69 -0.15453707021636376 1102 70-71 -0.16020632245012933 1102 72-73 -0.04428922160561655 1102 74-75 -0.03270703073432202 1102 76-77 0.03429640323501815 1102 78-79 -0.07070302011427287 1102 80-81 -0.02928237241122389 1102 82-83 0.00658484314097052 1102 84-85 0.15102928072704103 1102 86-87 0.33889023293948384 1102 88-89 0.5766682103012553 1102 90-91 -0.02322112566165746 1102 92-93 -0.11186578764745736 1102 94-95 0.013512284611234548 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.016847731871060034 1103 2 0.21876798807507214 1103 3 0.2659749107751779 1103 4 0.32646971258410673 1103 5 0.15594391023817167 1103 6 -0.04386262308986488 1103 7 -0.020311170521978283 1103 8 0.1744978041943952 1103 9 0.34044214879037327 1103 10-11 0.4410708432985331 1103 12-13 0.1999158084926549 1103 14-15 0.1351392914525391 1103 16-17 0.2077800304009969 1103 18-19 0.30962005578513185 1103 20-21 0.2819325154554022 1103 22-23 0.3420975829241044 1103 24-25 0.36686228050590586 1103 26-27 0.32766771232000735 1103 28-29 0.26962599145151955 1103 30-31 0.16288287336332274 1103 32-33 0.22605746270141935 1103 34-35 0.06342414060243584 1103 36-37 0.15537525383948037 1103 38-39 0.3451476566960707 1103 40-41 0.22025275815408207 1103 42-43 0.29428338899447937 1103 44-45 0.14086056711386163 1103 46-47 0.19155559571849068 1103 48-49 0.2604707364016434 1103 50-51 0.10032031124401897 1103 52-53 0.017150866539559217 1103 54-55 0.2955775629347315 1103 56-57 0.25939013009382705 1103 58-59 0.08729308964734628 1103 60-61 -0.10560635730011114 1103 62-63 -0.10897966381921265 1103 64-65 -0.23410370547695436 1103 66-67 -0.16578508633010358 1103 68-69 -0.1354996105009434 1103 70-71 -0.12462608348005944 1103 72-73 -0.07550941384275234 1103 74-75 -0.010023499576213624 1103 76-77 -0.0033354564307472856 1103 78-79 0.18703077151782566 1103 80-81 0.2354895788013991 1103 82-83 0.3802591913915947 1103 84-85 0.42511395311679934 1103 86-87 0.4641068242577724 1103 88-89 0.2211452022358742 1103 90-91 -0.6194751541190753 1103 92-93 -0.27530404430605326 1103 94-95 -0.09544655316116257 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.07951108172913024 1104 2 0.10745860780017935 1104 3 -0.0769557218106236 1104 4 0.04191059354222659 1104 5 0.19370614800040897 1104 6 0.045101286563699716 1104 7 0.15703791717193383 1104 8 0.08008417184972672 1104 9 0.09295400475050286 1104 10-11 0.12673115999333362 1104 12-13 -0.14819125685578882 1104 14-15 0.07177621774464171 1104 16-17 0.1989704491776223 1104 18-19 0.1851364933705355 1104 20-21 0.10783075514674323 1104 22-23 0.18448163565298614 1104 24-25 0.4111111350995813 1104 26-27 0.44445092910322614 1104 28-29 0.3475457728195721 1104 30-31 0.3413492345883 1104 32-33 0.3916952731668175 1104 34-35 0.5050445891194357 1104 36-37 0.31529808962437755 1104 38-39 0.46069303086213154 1104 40-41 0.52197287711558 1104 42-43 0.4546885011237336 1104 44-45 0.24368992166736092 1104 46-47 0.30272833102915087 1104 48-49 0.22129382136265718 1104 50-51 0.19831886441498625 1104 52-53 0.19667823572209642 1104 54-55 0.2623567607484105 1104 56-57 0.08999056414253914 1104 58-59 0.16961766162709324 1104 60-61 0.21052578641823771 1104 62-63 0.2789634275721511 1104 64-65 0.2629624467616125 1104 66-67 0.25017826066772386 1104 68-69 -0.04337679079191048 1104 70-71 -0.2223031190881315 1104 72-73 0.06179466139235501 1104 74-75 0.016156067982670663 1104 76-77 -0.2947509375013908 1104 78-79 -0.0015232032430532172 1104 80-81 -0.2405010971892736 1104 82-83 -0.2020734185272275 1104 84-85 -0.06767601553523761 1104 86-87 0.11716526007482742 1104 88-89 1.0329288209160463 1104 90-91 -0.6290322636761849 1104 92-93 -0.27530404430605326 1104 94-95 -0.09544655316116257 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.19629803708865978 1105 2 -0.14883650045964458 1105 3 0.03528699404075297 1105 4 0.0602746673192911 1105 5 -0.024123309363936585 1105 6 0.2393541601269149 1105 7 0.056102338449669276 1105 8 -0.1728161221892961 1105 9 0.22158271365186977 1105 10-11 0.11966085793505954 1105 12-13 0.27223109011026736 1105 14-15 -0.01566029655634793 1105 16-17 -0.016573523022330505 1105 18-19 0.05936798227724438 1105 20-21 0.1316479163335913 1105 22-23 -0.09203533841812117 1105 24-25 -0.01966940486298796 1105 26-27 0.10530364228151967 1105 28-29 0.08702719489923538 1105 30-31 -0.009933844802240799 1105 32-33 -0.08496872041778403 1105 34-35 -0.07374450470574345 1105 36-37 -0.2847152757859419 1105 38-39 -0.10920806510151237 1105 40-41 -0.02906962488923881 1105 42-43 0.05543696410154553 1105 44-45 0.11769419859865593 1105 46-47 0.2035168902379212 1105 48-49 0.227121140261346 1105 50-51 0.1931999122631538 1105 52-53 0.2522649772350647 1105 54-55 0.10024769900022079 1105 56-57 0.0193944668423498 1105 58-59 0.014351689961763725 1105 60-61 0.1864546824353468 1105 62-63 0.14751461709366964 1105 64-65 0.3326496961601677 1105 66-67 0.579542067136579 1105 68-69 0.5061955160779092 1105 70-71 0.440300462841833 1105 72-73 0.5832996065941671 1105 74-75 0.5949718926284859 1105 76-77 0.4481119333887378 1105 78-79 0.4544645005794621 1105 80-81 0.4715144066867012 1105 82-83 0.7002922590990792 1105 84-85 0.7881513033634526 1105 86-87 0.7496031109168477 1105 88-89 1.5626668588738095 1105 90-91 -0.729032263676185 1105 92-93 -0.27530404430605326 1105 94-95 -0.09544655316116257 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.35981482126208064 1106 2 -0.3511620818549943 1106 3 -0.23525914928240965 1106 4 -0.2754088010591147 1106 5 -0.12137680825902208 1106 6 -0.20182231046131704 1106 7 -0.5991538964235303 1106 8 -0.22252673988896987 1106 9 -0.6695674494545187 1106 10-11 -0.44395589466937935 1106 12-13 -0.5325673945787841 1106 14-15 -0.4779143216010766 1106 16-17 -0.4656054076903189 1106 18-19 -0.27614711821733096 1106 20-21 -0.38116908882268064 1106 22-23 -0.3757825761325293 1106 24-25 -0.3530746453133773 1106 26-27 -0.6553982413341828 1106 28-29 -0.33043571155135965 1106 30-31 -0.30554575683001417 1106 32-33 -0.22560851837632612 1106 34-35 -0.32859560435848323 1106 36-37 -0.056403163821293845 1106 38-39 -0.058355702689652844 1106 40-41 -0.21009140748420663 1106 42-43 -0.5759689077284023 1106 44-45 -0.11027065460559271 1106 46-47 -0.47730495549500773 1106 48-49 -0.4158284546034281 1106 50-51 -0.37434717812935503 1106 52-53 -0.15979963394035934 1106 54-55 -0.21662591985698043 1106 56-57 -0.4500572852359426 1106 58-59 -0.289952214068542 1106 60-61 -0.1392687733154716 1106 62-63 0.09193216944555616 1106 64-65 -0.04597810558664861 1106 66-67 -0.11402312280343807 1106 68-69 0.041142374964579176 1106 70-71 -0.13720664017826323 1106 72-73 -0.15867134994587673 1106 74-75 -0.06223109590145981 1106 76-77 -0.011048331790675547 1106 78-79 -0.18185034736329442 1106 80-81 0.05308864640047517 1106 82-83 -0.056426671764860714 1106 84-85 -0.14868969421626232 1106 86-87 -0.46140393948828873 1106 88-89 0.03133990948940557 1106 90-91 -0.18604583833681865 1106 92-93 -0.27530404430605326 1106 94-95 -0.09544655316116257 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.11698591415770565 1107 2 -0.10615099538049222 1107 3 -0.014086491237442544 1107 4 0.09763350813814498 1107 5 -0.020188900040984947 1107 6 -0.16530215916355218 1107 7 -0.09376617080624783 1107 8 -0.052973911082197844 1107 9 -0.13940320910376158 1107 10-11 -0.18207430423686333 1107 12-13 0.01832504153603054 1107 14-15 -0.025756723102006163 1107 16-17 -0.1788820879684465 1107 18-19 -0.2721640138038097 1107 20-21 -0.2839040869665652 1107 22-23 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30-31 0.21399654524448408 2216 32-33 -0.007300601356419634 2216 34-35 -0.010515463811202608 2216 36-37 -0.04743510359841707 2216 38-39 0.139230173126208 2216 40-41 0.1380388847438141 2216 42-43 0.07903400331620247 2216 44-45 0.2018954690065513 2216 46-47 0.009855805666553863 2216 48-49 0.02172453151548126 2216 50-51 0.037420081300894026 2216 52-53 0.15685051978322662 2216 54-55 0.2608466805740548 2216 56-57 -0.008722494677940063 2216 58-59 -0.07773450878882926 2216 60-61 -0.017101934709906175 2216 62-63 -0.2532334536712 2216 64-65 -0.1712245229589513 2216 66-67 -0.2902332491529833 2216 68-69 -0.4684461973978671 2216 70-71 -0.2603872372999021 2216 72-73 0.05322716184641507 2216 74-75 -0.2243073346275466 2216 76-77 -0.16425354511406454 2216 78-79 0.021006051317030483 2216 80-81 -0.04810079621109509 2216 82-83 -0.21771131064211602 2216 84-85 -0.14602634375713208 2216 86-87 0.17446268359782735 2216 88-89 -0.652533414106399 2216 90-91 -0.729032263676185 2216 92-93 -0.27530404430605326 2216 94-95 -0.09544655316116257 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3183.0 3 552.0 4 535.0 5 927.0 6 651.0 7 441.0 8 462.0 9 513.0 10 586.0 11 701.0 12 937.0 13 1095.0 14 1232.0 15 1412.0 16 1685.0 17 1978.0 18 2530.0 19 3219.0 20 3106.0 21 4302.0 22 5273.0 23 6408.0 24 10081.0 25 17696.0 26 29821.0 27 70944.0 28 112771.0 29 4109.0 30 177.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.81045637896891 20.284901871386957 14.068291528467563 22.83635022117657 2 12.556600063343346 21.612262157919833 44.98247616793643 20.84866161080039 3 21.1376226006483 26.4587605714226 28.74626148537845 23.65735534255065 4 10.866219594571067 17.719932312449078 40.92172060083147 30.492127492148384 5 11.094668890428656 40.275625781247285 34.46436467839597 14.1653406499281 6 27.4617421961737 37.75489250923164 20.65416755125693 14.129197743337729 7 27.440163994333982 32.76858770668959 22.250954487395894 17.540293811580536 8 22.890295725775857 38.78403352278067 20.572379205574137 17.75329154586934 9 24.613419553331223 20.62040810644318 23.102945424551123 31.663226915674475 10-11 23.735532472866044 26.652791530134046 30.364796597575218 19.246879399424692 12-13 27.561172124201033 24.971069296213862 28.5141016516169 18.953656927968204 14-15 20.411412780560127 31.190768706038764 26.536141747903958 21.86167676549715 16-17 18.83658486077685 31.581493527398273 30.906825510351467 18.67509610147341 18-19 20.09905694033786 30.557465033352532 30.714612638983397 18.62886538732621 20-21 21.618700904556892 29.401927447506814 27.807380543847867 21.17199110408843 22-23 19.50363002304003 32.28493070588809 28.433730327224126 19.777708943847756 24-25 21.565324525714605 29.64618709693137 30.269170666174777 18.519317711179248 26-27 19.159877073856617 30.88049504571446 30.887977809255656 19.071650071173263 28-29 18.226256375631035 32.97595575734057 27.530892771065467 21.266895095962926 30-31 20.06094101842152 29.63661612030892 29.384116355232887 20.918326506036674 32-33 18.969111633168016 31.233446445662576 30.745149221265116 19.05229269990429 34-35 19.9848952587122 31.069652347325523 30.264472186741937 18.680980207220344 36-37 18.95157781900065 30.05147445245313 30.89441646625622 20.102531262290004 38-39 20.073122261395554 31.951922374159057 29.1029036602895 18.872051704155894 40-41 21.867593369227396 29.544212691463034 30.256989423200743 18.331204516108823 42-43 18.33016040956819 32.42316245949737 29.724495087478726 19.522182043455715 44-45 18.830983513557722 31.34233817218709 28.971346236169936 20.855332078085247 46-47 21.424370142729366 29.91608863768459 29.120131418209915 19.53940980137613 48-49 20.192359563075456 30.38651151216473 29.21032344736737 20.210805477392444 50-51 17.523936142443976 32.33093304840826 30.542900597576978 19.602230211570788 52-53 18.20364950039502 31.694550111893417 30.01023224409819 20.091568143613376 54-55 18.45104368784206 29.818583647295288 30.479861479696158 21.25051118516649 56-57 20.161523281835677 29.883025263897927 28.01755491130315 21.937896542963244 58-59 21.321699666233943 30.856828630793487 27.09038830322246 20.731083399750112 60-61 19.397933365120576 28.913920376435208 32.17292492525937 19.515221333184837 62-63 19.434303076285904 29.290494802089604 30.875796566281622 20.399405555342867 64-65 20.01969881006658 31.77355417346786 28.042091415007985 20.164655601457575 66-67 20.297431149874534 31.907547846182226 26.97762479683427 20.817396207108974 68-69 20.75722218451831 29.46700008526885 29.96051530227807 19.815262427934773 70-71 22.6955698559481 29.348790750608195 28.543436572267833 19.41220282117587 72-73 20.9045791032517 30.383500332373913 29.3369575431477 19.374963021226684 74-75 19.12176718512357 32.57873433405145 29.878500802221858 18.420997678603122 76-77 20.142729364104316 29.426054634614918 30.44162226313573 19.98959373814504 78-79 19.80151500122683 29.56149189162851 29.910224083055336 20.72676902408932 80-81 21.014036272261222 30.303278146502073 29.329822815120053 19.352862766116655 82-83 20.04517516688361 29.74374055950384 31.398829204457655 18.812255069154897 84-85 19.392364796903877 30.4195220080257 30.940357154043994 19.247756041026427 86-87 18.084145571107133 32.89410716492038 28.211580326704915 20.810166937267574 88-89 28.61547850041149 19.845674834168133 35.647566187704264 15.891280477716109 90-91 30.813614599139587 15.877484654903471 17.006921517589337 36.30197922836761 92-93 22.34490409599159 18.135345809126207 36.11216535777888 23.40758473710332 94-95 15.99898133708939 7.413287891745057 28.434528899844057 48.15320187132149 96-97 27.335733144614714 7.45018917226925 15.670676937314571 49.54340074580146 98-99 56.488646777313924 14.099887995112514 19.053558700743306 10.357906526830261 100-101 31.014065005101298 7.281737356070543 12.89352864013992 48.810668998688236 102-103 23.582361270312184 2.1887338083144163 37.850523303291155 36.378381618082244 104-105 41.41735448415421 4.791506406924607 42.210163759468315 11.580975349452864 106-107 6.109797345041031 2.216862475386514 7.170650361311839 84.5026898182606 108-109 17.826178727742665 11.820730633978103 22.627510483996225 47.725580154283 110-111 40.70630283088444 2.9922845634537927 26.062007148125037 30.239405457536733 112-113 27.629017795306588 2.8409795073302666 39.79813517434443 29.731867523018714 114-115 29.892400369507797 4.1998985154633806 24.52803185053149 41.37966926449732 116-117 20.643974814537746 4.601749683103713 21.151008873096025 53.603266629262514 118-119 20.674269974549865 4.3189073576918435 16.482295970500687 58.524526697257606 120-121 26.2550191844383 4.536450432765236 30.099937539038102 39.108592843758366 122-123 34.75775999066906 1.7138316639694413 34.380148420300635 29.14825992506087 124-125 39.519252357431526 2.2991973506960037 20.552452851369555 37.62909744050292 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 43.0 1 84.0 2 197.0 3 304.0 4 365.5 5 357.5 6 277.5 7 192.0 8 134.0 9 106.5 10 86.0 11 65.5 12 51.5 13 47.0 14 48.0 15 61.0 16 83.5 17 106.0 18 146.0 19 218.5 20 334.5 21 441.5 22 626.0 23 879.0 24 1162.5 25 1558.5 26 1997.0 27 2605.5 28 3473.0 29 4426.0 30 5832.0 31 7577.5 32 9587.0 33 12034.5 34 14154.0 35 15677.0 36 16270.0 37 15806.5 38 14979.5 39 14016.5 40 12914.0 41 11768.0 42 10966.0 43 11040.5 44 11432.5 45 11020.0 46 9761.5 47 8673.0 48 8209.5 49 7899.0 50 7483.0 51 6575.0 52 5301.5 53 4095.5 54 3115.5 55 2495.5 56 1989.0 57 1548.5 58 1235.0 59 927.5 60 671.5 61 514.0 62 368.5 63 258.0 64 176.5 65 127.5 66 102.5 67 72.5 68 44.5 69 31.0 70 24.5 71 21.0 72 13.5 73 9.0 74 8.5 75 10.0 76 9.5 77 3.5 78 1.5 79 1.5 80 0.5 81 0.5 82 1.0 83 1.5 84 2.0 85 1.0 86 0.5 87 1.5 88 1.0 89 1.0 90 2.0 91 1.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.001392142054175208 3 0.03967604854399343 4 0.04350443919297525 5 0.043852474706519054 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.700887838595051E-4 12-13 8.700887838595051E-4 14-15 0.0 16-17 1.74017756771901E-4 18-19 0.006090621487016535 20-21 0.001392142054175208 22-23 3.48035513543802E-4 24-25 0.0 26-27 0.0 28-29 8.700887838595051E-4 30-31 0.0 32-33 6.96071027087604E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.74017756771901E-4 50-51 0.0 52-53 0.0 54-55 0.001566159810947109 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.74017756771901E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.74017756771901E-4 80-81 0.0 82-83 3.48035513543802E-4 84-85 0.0 86-87 0.001218124297403307 88-89 19.015442335735937 90-91 26.259801550150176 92-93 40.39369777292075 94-95 44.65121621010208 96-97 48.85409307165703 98-99 93.16388644297264 100-101 52.24256683151949 102-103 53.54456768768685 104-105 74.88975975108501 106-107 81.52958127847366 108-109 12.609326655691946 110-111 38.65178002763402 112-113 52.25962057168313 114-115 86.62516923226846 116-117 66.50297396346323 118-119 72.58124018974897 120-121 80.49782999857305 122-123 76.12859216154416 124-125 50.39519432562899 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 287327.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.99230387611622 #Duplication Level Percentage of deduplicated Percentage of total 1 91.16758199377026 70.19202176515101 2 6.521471986976486 10.042063058817462 3 1.1226399651037269 2.5930391201021594 4 0.4022038257852529 1.238663966999788 5 0.18946925037679557 0.7293837050095097 6 0.11438486518204397 0.5284052579354709 7 0.0724501025936023 0.3904670220319697 8 0.06403039076816551 0.39438778426632537 9 0.03913292305545451 0.2711640513101605 >10 0.2514991682803549 3.6355808869556596 >50 0.02666390293739539 1.3925028983985954 >100 0.02214459735478483 3.290579687694898 >500 0.004067375024348234 2.0445644755096986 >1k 0.0022596527913045744 3.2571763198172716 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3904 1.3587306448750032 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2003 0.6971151336282354 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1242 0.4322601078214021 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1140 0.39676048543993425 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1072 0.37309407051895577 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 708 0.2464091435890118 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 700 0.2436248594806614 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 693 0.24118861088585478 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 692 0.24084057537231096 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 668 0.23248772304725976 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 666 0.23179165202017213 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 639 0.22239469315448948 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 581 0.20220863336894898 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 529 0.18411078666467126 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 438 0.15243955493218528 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 432 0.15035134185092247 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 362 0.12598885590285633 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 345 0.1200722521726117 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 321 0.11171939984756045 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 305 0.10615083163085962 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.48035513543802E-4 0.0 10-11 0.0 0.0 0.0 3.48035513543802E-4 0.0 12-13 0.0 0.0 0.0 5.22053270315703E-4 0.0 14-15 0.0 0.0 0.0 6.96071027087604E-4 0.0 16-17 0.0 0.0 0.0 0.0010441065406314061 0.0 18-19 0.0 0.0 0.0 0.0010441065406314061 0.0 20-21 0.0 0.0 0.0 0.0010441065406314061 0.0 22-23 0.0 0.0 0.0 0.0017401775677190101 0.0 24-25 0.0 0.0 0.0 0.0022622308380347133 0.0 26-27 0.0 0.0 0.0 0.002610266351578515 0.0 28-29 0.0 0.0 0.0 0.003306337378666119 0.0 30-31 0.0 0.0 0.0 0.005916603730244634 0.0 32-33 0.0 0.0 0.0 0.010963118676629763 0.0 34-35 0.0 0.0 0.0 0.01583561586624299 0.0 36-37 0.0 0.0 0.0 0.02766882332673226 0.0 38-39 0.0 0.0 0.0 0.040894172841396736 0.0 40-41 0.0 0.0 0.0 0.06473460551914717 0.0 42-43 0.0 0.0 0.0 0.08422459427760008 0.0 44-45 0.0 0.0 0.0 0.10684690265794722 0.0 46-47 0.0 0.0 0.0 0.1355598325253109 0.0 48-49 0.0 0.0 0.0 0.16601293996039357 0.0 50-51 0.0 0.0 0.0 0.1929856922600382 0.0 52-53 0.0 0.0 0.0 0.2243088884789804 0.0 54-55 0.0 0.0 0.0 0.265377079077149 0.0 56-57 0.0 0.0 0.0 0.3074893762159491 0.0 58-59 0.0 0.0 0.0 0.36334907613972933 0.0 60-61 0.0 0.0 0.0 0.4258214508208418 0.0 62-63 0.0 0.0 0.0 0.4867276656910071 0.0 64-65 0.0 0.0 0.0 0.5450236142095939 0.0 66-67 0.0 0.0 0.0 0.627508030919475 0.0 68-69 0.0 0.0 0.0 0.72774225882009 0.0 70-71 0.0 0.0 0.0 0.8452042446411232 0.0 72-73 0.0 0.0 0.0 0.9377816912437745 0.0 74-75 0.0 0.0 0.0 1.0326213686844605 0.0 76-77 0.0 0.0 0.0 1.1542597806680193 0.0 78-79 0.0 0.0 0.0 1.186627083427593 0.0 80-81 0.0 0.0 0.0 1.1876711899682244 0.0 82-83 0.0 0.0 0.0 1.1878452077249964 0.0 84-85 0.0 0.0 0.0 1.1878452077249964 0.0 86-87 0.0 0.0 0.0 1.1878452077249964 0.0 88-89 0.0 0.0 0.0 1.1878452077249964 0.0 90-91 0.0 0.0 0.0 1.1878452077249964 0.0 92-93 0.0 0.0 0.0 1.1878452077249964 0.0 94-95 0.0 0.0 0.0 1.1878452077249964 0.0 96-97 0.0 0.0 0.0 1.1878452077249964 0.0 98-99 0.0 0.0 0.0 1.1878452077249964 0.0 100-101 0.0 0.0 0.0 1.1878452077249964 0.0 102-103 0.0 0.0 0.0 1.1878452077249964 0.0 104-105 0.0 0.0 0.0 1.1878452077249964 0.0 106-107 0.0 0.0 0.0 1.1878452077249964 0.0 108-109 0.0 0.0 0.0 1.1878452077249964 0.0 110-111 0.0 0.0 0.0 1.1878452077249964 0.0 112-113 0.0 0.0 0.0 1.1878452077249964 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGCGCG 5 2.4137516E-4 5791.0117 110-111 CGCCGCC 5 2.4137516E-4 5791.0117 110-111 CCGCTCG 10 1.5697697E-7 5127.458 108-109 CAGCTCG 5 3.0830654E-4 5127.458 108-109 TCGCGTG 5 3.0830654E-4 5127.458 108-109 CCGCTCC 60 0.0 2991.017 108-109 CTCCGAT 10 9.653921E-4 2895.5059 110-111 CGTTGGT 10 9.653921E-4 2895.5059 110-111 CTGCGCG 10 9.653921E-4 2895.5059 110-111 CTCGATG 10 9.653921E-4 2895.5059 110-111 CGCGTTG 10 9.653921E-4 2895.5059 110-111 CGCGTCT 10 9.653921E-4 2895.5059 110-111 CGGCGCG 10 0.0012330691 2563.729 108-109 CGACGCC 10 0.0012330691 2563.729 108-109 CCGCGCC 40 1.8189894E-12 2563.729 108-109 CCTCTCG 35 2.222805E-9 2197.4822 108-109 CTCCGCC 45 3.7034624E-9 1930.3373 110-111 CGTGTTG 15 0.0021718882 1930.3372 110-111 GTGCGCT 15 0.0021718882 1930.3372 110-111 CTGCTCG 15 0.0027740526 1709.1527 108-109 >>END_MODULE