##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939909_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 287327 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74735754036342 34.0 31.0 34.0 31.0 34.0 2 32.846241390471484 34.0 31.0 34.0 31.0 34.0 3 32.89606963494555 34.0 31.0 34.0 31.0 34.0 4 36.29182081739621 37.0 37.0 37.0 35.0 37.0 5 36.21613701462097 37.0 37.0 37.0 35.0 37.0 6 36.38200377966568 37.0 37.0 37.0 35.0 37.0 7 36.38597486487521 37.0 37.0 37.0 35.0 37.0 8 36.4132956526884 37.0 37.0 37.0 35.0 37.0 9 38.231363568338516 39.0 39.0 39.0 37.0 39.0 10-11 38.21749087276866 39.0 39.0 39.0 37.0 39.0 12-13 38.21477793594059 39.0 39.0 39.0 37.0 39.0 14-15 39.68454409087903 41.0 40.0 41.0 37.0 41.0 16-17 39.709839312003396 41.0 40.0 41.0 37.0 41.0 18-19 39.78510721233995 41.0 40.0 41.0 37.5 41.0 20-21 39.80964893657749 41.0 40.0 41.0 37.5 41.0 22-23 39.74629603204711 41.0 40.0 41.0 37.0 41.0 24-25 39.69801654560831 41.0 40.0 41.0 37.0 41.0 26-27 39.572497537648744 41.0 40.0 41.0 36.5 41.0 28-29 39.50348731584571 41.0 40.0 41.0 36.0 41.0 30-31 39.37640736860789 41.0 39.0 41.0 36.0 41.0 32-33 39.19828627313131 41.0 39.0 41.0 35.0 41.0 34-35 39.064692841257525 41.0 39.0 41.0 35.0 41.0 36-37 39.03480529153195 41.0 39.0 41.0 35.0 41.0 38-39 39.073082237311496 41.0 39.0 41.0 35.0 41.0 40-41 39.00044374527977 41.0 39.0 41.0 35.0 41.0 42-43 38.90289461136614 41.0 39.0 41.0 35.0 41.0 44-45 38.74681460496229 41.0 39.0 41.0 35.0 41.0 46-47 38.4780598412262 41.0 39.0 41.0 35.0 41.0 48-49 38.203190093517144 41.0 39.0 41.0 35.0 41.0 50-51 38.016429016416836 41.0 39.0 41.0 34.0 41.0 52-53 37.95581863173318 41.0 39.0 41.0 34.0 41.0 54-55 37.87110852791419 41.0 38.5 41.0 34.0 41.0 56-57 37.712225095448744 40.5 38.0 41.0 33.5 41.0 58-59 37.55500353256046 40.0 38.0 41.0 33.0 41.0 60-61 37.31703947070759 40.0 38.0 41.0 33.0 41.0 62-63 37.18418735447765 40.0 37.0 41.0 33.0 41.0 64-65 36.95577512729399 39.5 37.0 41.0 32.0 41.0 66-67 36.659692615034444 39.0 36.5 41.0 32.0 41.0 68-69 36.279731803833265 39.0 36.0 41.0 31.0 41.0 70-71 35.85004541863452 38.5 35.0 40.5 31.0 41.0 72-73 35.488316447810334 37.0 35.0 40.0 30.5 41.0 74-75 35.16330696384259 37.0 35.0 39.0 30.5 41.0 76-77 34.77251876781507 36.5 35.0 39.0 30.0 41.0 78-79 34.378029214101005 36.0 35.0 39.0 30.0 40.0 80-81 34.057944432649904 36.0 35.0 37.0 29.5 39.0 82-83 33.78296157339895 35.0 35.0 37.0 29.5 39.0 84-85 33.477327226470194 35.0 34.0 37.0 29.0 39.0 86-87 33.29070884393043 35.0 34.0 36.0 29.5 37.0 88-89 33.03439983015867 35.0 34.0 36.0 29.0 37.0 90-91 32.793592318160144 35.0 34.0 36.0 29.0 37.0 92-93 32.64324619684193 35.0 34.0 35.0 29.0 36.0 94-95 32.5163176450525 35.0 34.0 35.0 29.0 36.0 96-97 32.341309727244564 35.0 34.0 35.0 29.0 36.0 98-99 32.221634235557396 35.0 34.0 35.0 28.0 36.0 100-101 32.217348178208105 35.0 34.0 35.0 28.5 36.0 102-103 31.30017192954369 34.0 32.0 35.0 25.5 35.0 104-105 31.97975303399959 35.0 34.0 35.0 27.0 35.0 106-107 31.995075297483353 35.0 34.0 35.0 27.0 35.0 108-109 32.044162226313574 35.0 34.0 35.0 27.0 35.0 110-111 32.05158756399503 35.0 34.0 35.0 27.5 35.0 112-113 31.934226508472925 35.0 34.0 35.0 27.0 35.0 114-115 31.822009417840995 35.0 34.0 35.0 25.0 35.0 116-117 31.739860855401687 35.0 34.0 35.0 25.0 35.0 118-119 31.635914828749126 35.0 34.0 35.0 25.0 35.0 120-121 31.5439882085568 35.0 34.0 35.0 25.0 35.0 122-123 31.334956338944828 35.0 34.0 35.0 24.5 35.0 124-125 29.174261381631382 33.0 29.5 34.5 10.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08336672333032169 1101 2 0.11180611985309952 1101 3 0.1965841884672841 1101 4 -0.00829640724692382 1101 5 0.07671050420820791 1101 6 0.17475932434069108 1101 7 0.09932108275403806 1101 8 0.16272028248129544 1101 9 0.07381121008736358 1101 10-11 0.12750948458221245 1101 12-13 0.09412111690298275 1101 14-15 0.25951023067676715 1101 16-17 0.25516739712426073 1101 18-19 0.26636225535303026 1101 20-21 0.2654624015140996 1101 22-23 0.21390223682207932 1101 24-25 0.10577173182242916 1101 26-27 0.19432028706464166 1101 28-29 0.15929100150366793 1101 30-31 0.22905676953453025 1101 32-33 0.2895417997061429 1101 34-35 0.24224839979588353 1101 36-37 0.1105994813778679 1101 38-39 0.08821138080245561 1101 40-41 0.08526726487473724 1101 42-43 0.07199357309563226 1101 44-45 0.20363943078911717 1101 46-47 0.4340835456118626 1101 48-49 0.22588339528763157 1101 50-51 0.20072441839879218 1101 52-53 0.051931883723185024 1101 54-55 0.24249860728145478 1101 56-57 0.41391644883803025 1101 58-59 0.22705713926923465 1101 60-61 0.31079974866593574 1101 62-63 0.3814188058451009 1101 64-65 0.36319002890297725 1101 66-67 0.5524292539299083 1101 68-69 0.39995840751126366 1101 70-71 0.4106269070718582 1101 72-73 0.5529462709412343 1101 74-75 0.4764833341665664 1101 76-77 0.5107754222356533 1101 78-79 0.4676294884707488 1101 80-81 0.4149837512041259 1101 82-83 0.2909006997339034 1101 84-85 0.30154116147081567 1101 86-87 0.4956328381356485 1101 88-89 0.2981832918209619 1101 90-91 0.20600567845632156 1101 92-93 0.351525325357656 1101 94-95 0.18679246386496828 1101 96-97 0.21921237473046773 1101 98-99 0.2483704512564131 1101 100-101 0.25451579596059304 1101 102-103 0.33588132715325614 1101 104-105 0.2733954722784233 1101 106-107 0.39303856679105564 1101 108-109 0.2429857762964076 1101 110-111 0.07472347491336961 1101 112-113 0.13036123050311232 1101 114-115 -0.0046033057923331455 1101 116-117 0.27095002933786816 1101 118-119 0.11867995614045057 1101 120-121 -0.0688205757255993 1101 122-123 -0.30046539275742745 1101 124-125 -0.15667546273480326 1102 1 0.13141899508648436 1102 2 0.23104364537177702 1102 3 0.12783804594247528 1102 4 -0.07218213102795801 1102 5 -0.15920674404702595 1102 6 0.017991532478184524 1102 7 -0.008189718176502936 1102 8 -0.03780908180679177 1102 9 0.1259108635080537 1102 10-11 0.17671306431919476 1102 12-13 0.13505783057150467 1102 14-15 0.16456589701110147 1102 16-17 0.2519508562706321 1102 18-19 0.20883839835261142 1102 20-21 0.19436913369850117 1102 22-23 0.2203115802204536 1102 24-25 0.2510027055678634 1102 26-27 0.28424693569019155 1102 28-29 0.15380448486310883 1102 30-31 0.28970221415305275 1102 32-33 0.35626128411006874 1102 34-35 0.24345608626030213 1102 36-37 0.15001100870066608 1102 38-39 0.16584455271529208 1102 40-41 0.1847426482484238 1102 42-43 0.3348389654898867 1102 44-45 0.47053220484789193 1102 46-47 0.3528124778627273 1102 48-49 0.21590737097960755 1102 50-51 0.22104144351392563 1102 52-53 0.2192394847296839 1102 54-55 0.393750209617302 1102 56-57 0.42682712158170233 1102 58-59 0.40273249415695744 1102 60-61 0.4232955944618837 1102 62-63 0.3105332482322254 1102 64-65 0.24198815832423293 1102 66-67 0.29471133625435897 1102 68-69 0.03741805238615825 1102 70-71 0.06522264367157504 1102 72-73 0.2560096997025667 1102 74-75 0.19396481828563594 1102 76-77 0.2387165987062403 1102 78-79 0.19730949606700676 1102 80-81 0.20598574522207969 1102 82-83 0.3566261424533579 1102 84-85 0.2074632760793662 1102 86-87 0.21629525564502927 1102 88-89 0.26414849146014063 1102 90-91 0.27668352586646705 1102 92-93 0.43674585424717804 1102 94-95 0.4304929101451762 1102 96-97 0.3968372382351859 1102 98-99 0.36261699733247355 1102 100-101 0.13631682145553015 1102 102-103 0.3234869626277863 1102 104-105 0.4060807497792567 1102 106-107 0.4128303818221539 1102 108-109 0.5133146705659826 1102 110-111 0.4066443788681795 1102 112-113 0.5730747565915166 1102 114-115 0.5997176598820708 1102 116-117 0.5629401304630655 1102 118-119 0.6464834504195203 1102 120-121 0.6683251300143205 1102 122-123 0.6961845384014786 1102 124-125 0.7770056368236666 1103 1 0.012391130295910102 1103 2 0.05359962047013056 1103 3 0.13680083456628012 1103 4 0.11334420704075399 1103 5 -0.02590501752496266 1103 6 0.033665124422967097 1103 7 0.02452333148570318 1103 8 0.004589197879624862 1103 9 -0.06424925885546173 1103 10-11 0.09125897039051978 1103 12-13 0.03540042582934433 1103 14-15 0.07351852630271338 1103 16-17 0.11698695012028537 1103 18-19 -0.014712062485990884 1103 20-21 0.10807689486389194 1103 22-23 0.07081982903379469 1103 24-25 0.16315552450033977 1103 26-27 0.2765738178475843 1103 28-29 0.2158949367840748 1103 30-31 0.13487399152821666 1103 32-33 0.15838006927970838 1103 34-35 0.3417702015235733 1103 36-37 0.33195900420798097 1103 38-39 0.24336872345980254 1103 40-41 0.2490201786276458 1103 42-43 0.2788271806645426 1103 44-45 0.3130851321465826 1103 46-47 0.23554556398564586 1103 48-49 0.6212111067918116 1103 50-51 0.5406822546801635 1103 52-53 0.5657122210329248 1103 54-55 0.789727114068782 1103 56-57 0.7372144743419398 1103 58-59 0.5357435508968251 1103 60-61 0.777548686003108 1103 62-63 0.5523789731288034 1103 64-65 0.5017989689825058 1103 66-67 0.7915026805033349 1103 68-69 0.7179945380473924 1103 70-71 0.6336147035890605 1103 72-73 0.556288516930536 1103 74-75 0.5327616147095497 1103 76-77 0.34827370826334914 1103 78-79 0.5366340133576273 1103 80-81 0.45874146407948757 1103 82-83 0.6708712194102944 1103 84-85 0.5318733293029823 1103 86-87 0.5626748647365005 1103 88-89 0.8528761479844036 1103 90-91 0.7882849188237984 1103 92-93 0.9245405454317037 1103 94-95 0.7810180911788294 1103 96-97 0.5919585011530657 1103 98-99 0.7093775813811902 1103 100-101 0.8017700123324474 1103 102-103 0.6759762812187979 1103 104-105 0.6683975290451869 1103 106-107 0.6012690617862617 1103 108-109 0.688935659746285 1103 110-111 0.8177304679203701 1103 112-113 0.6451185320251227 1103 114-115 0.4640567106618043 1103 116-117 0.7054162983629659 1103 118-119 0.6320318003746443 1103 120-121 0.6487853477626722 1103 122-123 0.6738678720170164 1103 124-125 0.41316890858015753 1104 1 0.09616253130868557 1104 2 0.17461883114451382 1104 3 0.19401488488378504 1104 4 0.004397980853148908 1104 5 0.012934304072977909 1104 6 0.14527994638261532 1104 7 0.14306685692577759 1104 8 0.04497180377946819 1104 9 -0.13999879667396442 1104 10-11 0.026207929477408243 1104 12-13 0.17089596477315894 1104 14-15 0.31825769517980973 1104 16-17 0.22051560537307324 1104 18-19 0.1700640809108407 1104 20-21 0.2540780201753634 1104 22-23 0.28239446129808243 1104 24-25 0.18690763445934522 1104 26-27 0.19812353250074466 1104 28-29 0.11300127871799504 1104 30-31 -0.005307665894811464 1104 32-33 0.06608845285015974 1104 34-35 0.061543531641731875 1104 36-37 -0.06619226980536297 1104 38-39 0.022550461262227373 1104 40-41 0.04216381659887247 1104 42-43 -0.1905402335304629 1104 44-45 -0.2935461675881683 1104 46-47 -0.2777572251792151 1104 48-49 -0.5739982815210212 1104 50-51 -0.4049973530684099 1104 52-53 -0.4261614225648458 1104 54-55 -0.3123949018463392 1104 56-57 -0.24954704000577976 1104 58-59 -0.13457234010872554 1104 60-61 -0.12577902077287462 1104 62-63 0.021674045872146053 1104 64-65 0.005938507604803078 1104 66-67 -0.023576493196529213 1104 68-69 0.21849289026987861 1104 70-71 -0.04850088197480318 1104 72-73 0.008744107317156136 1104 74-75 -0.11012079024182242 1104 76-77 0.12607292054531882 1104 78-79 0.03460041477229936 1104 80-81 -0.12181476130432145 1104 82-83 0.034764797096976 1104 84-85 0.010018172965061467 1104 86-87 -0.06270894685420814 1104 88-89 -0.03837857430480085 1104 90-91 0.04284644924739922 1104 92-93 0.08499388519538087 1104 94-95 0.01300105074799518 1104 96-97 0.20252035309422212 1104 98-99 0.2311121686330182 1104 100-101 0.2131195823027099 1104 102-103 0.19371601275163286 1104 104-105 0.2286016926976231 1104 106-107 0.4014057067504879 1104 108-109 0.24801451192858792 1104 110-111 0.31437864732716747 1104 112-113 0.23068239345104757 1104 114-115 0.3077107577641236 1104 116-117 0.2835852558422651 1104 118-119 0.34628306635005046 1104 120-121 0.3676905804610122 1104 122-123 0.43776144970369657 1104 124-125 0.6240040504497841 1105 1 0.13620530062170388 1105 2 0.2468722393241407 1105 3 0.14140862987175495 1105 4 0.0682311805323792 1105 5 0.05041091460492453 1105 6 0.10480948393941958 1105 7 0.11892892589129644 1105 8 0.11992502484335432 1105 9 0.3167774663573155 1105 10-11 0.22660889178695243 1105 12-13 0.19060994499235306 1105 14-15 0.1031547815203595 1105 16-17 0.21622530866095957 1105 18-19 0.1800079461875086 1105 20-21 0.22339103808500482 1105 22-23 0.23517446664423858 1105 24-25 0.10068737249729764 1105 26-27 0.2815637891166247 1105 28-29 0.260755889784555 1105 30-31 0.2279053787456533 1105 32-33 0.30405958356386975 1105 34-35 0.3792126040822552 1105 36-37 0.14622419638015316 1105 38-39 0.14140905522106095 1105 40-41 0.071701373401865 1105 42-43 0.09813926393010775 1105 44-45 0.1094476843230936 1105 46-47 0.1280700937194723 1105 48-49 0.1681428572119401 1105 50-51 -0.12457767918442642 1105 52-53 -0.03543701732560578 1105 54-55 -0.03504927233550603 1105 56-57 0.05010545932594823 1105 58-59 0.0610972669482166 1105 60-61 0.2203984718761447 1105 62-63 0.17162058423087245 1105 64-65 0.24842714700602642 1105 66-67 0.18237111439502485 1105 68-69 0.2611018183639757 1105 70-71 0.3011649827672471 1105 72-73 0.32831106802285603 1105 74-75 0.31719810844837326 1105 76-77 0.45964683126437933 1105 78-79 0.3380353252240553 1105 80-81 0.19477171290043316 1105 82-83 0.37214784975669346 1105 84-85 0.2772194560444632 1105 86-87 0.14909271045667083 1105 88-89 0.09155994480774154 1105 90-91 0.19283308383707976 1105 92-93 0.08027589535309687 1105 94-95 0.2699376987030888 1105 96-97 0.2607935420811245 1105 98-99 0.22467004085969222 1105 100-101 0.3319781762615506 1105 102-103 0.293502166186542 1105 104-105 0.28552497524239584 1105 106-107 0.18365644184806484 1105 108-109 0.19424546621888084 1105 110-111 0.16813432762654656 1105 112-113 0.3194287179632127 1105 114-115 0.3169596217042532 1105 116-117 0.3846812194883462 1105 118-119 0.5722253277774776 1105 120-121 0.4601123928106503 1105 122-123 0.5495631070145741 1105 124-125 0.5142205700969349 1106 1 -0.039370833230762514 1106 2 -0.017907303977970912 1106 3 0.06837906026215279 1106 4 0.03827222020405685 1106 5 -0.03810850031668167 1106 6 0.03750442237991081 1106 7 0.008069197384507731 1106 8 -0.09331290002247528 1106 9 0.017061587161272485 1106 10-11 -0.023885688871786215 1106 12-13 0.06621764782304496 1106 14-15 -0.2451089246776803 1106 16-17 -0.04649172806427515 1106 18-19 0.05425818295483964 1106 20-21 8.507876377805701E-4 1106 22-23 0.07702440876778383 1106 24-25 0.1751533306157924 1106 26-27 0.1111754906845519 1106 28-29 0.06153459124725913 1106 30-31 -0.07090552307482056 1106 32-33 -0.05010752156082532 1106 34-35 0.06983135605531743 1106 36-37 -0.07360112194037782 1106 38-39 -0.20675468254595586 1106 40-41 -0.20847330827760402 1106 42-43 -0.24276887034327643 1106 44-45 -0.5247730880645776 1106 46-47 -0.4970061979778819 1106 48-49 -0.5612583845012082 1106 50-51 -0.4441662301683209 1106 52-53 -0.4307227165784795 1106 54-55 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-1.3588903598736266 2216 74-75 -1.4146250843701509 2216 76-77 -1.726294687975475 2216 78-79 -1.4894700556358345 2216 80-81 -1.6351589921234506 2216 82-83 -1.4472206210291674 2216 84-85 -1.5878023298760624 2216 86-87 -1.2573354485018058 2216 88-89 -1.4002244008272058 2216 90-91 -1.4580357281740497 2216 92-93 -1.5471822389321694 2216 94-95 -1.4496906282432072 2216 96-97 -1.4613441029396128 2216 98-99 -1.5049325629515238 2216 100-101 -1.519103245335387 2216 102-103 -1.3601279456303494 2216 104-105 -1.4882453014125439 2216 106-107 -1.6617737644619837 2216 108-109 -1.5255902206938146 2216 110-111 -1.7591741925057391 2216 112-113 -1.6046617854752867 2216 114-115 -1.7380301415379158 2216 116-117 -1.9443019292333723 2216 118-119 -1.904164508959358 2216 120-121 -1.769524333526693 2216 122-123 -1.941966632079339 2216 124-125 -2.197822056812612 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30.0 3 1.0 4 4.0 5 10.0 6 27.0 7 76.0 8 122.0 9 214.0 10 509.0 11 838.0 12 982.0 13 1542.0 14 2615.0 15 2855.0 16 1064.0 17 629.0 18 605.0 19 541.0 20 569.0 21 573.0 22 560.0 23 666.0 24 689.0 25 918.0 26 1138.0 27 1413.0 28 1827.0 29 2330.0 30 2896.0 31 3890.0 32 5036.0 33 6598.0 34 9782.0 35 16995.0 36 33538.0 37 96008.0 38 88015.0 39 1222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.84411429924012 20.126635593721826 14.659862643196034 22.36938746384202 2 12.970935554263956 21.669039108750656 44.489031660790666 20.87099367619472 3 21.521472051008082 26.220299519362953 29.18939048540513 23.068837944223826 4 11.02124060739158 17.608160736721576 40.750086138789605 30.620512517097243 5 10.657816800808334 39.78711543151807 34.72248353715898 14.832584230514618 6 27.113010611602807 38.06290393871791 20.114712505264038 14.709372944415247 7 26.643162668318677 32.94399760551567 22.58820090001984 17.82463882614582 8 23.22266964121019 37.46706713953092 20.54801671962607 18.762246499632823 9 24.878274579138054 19.199031069130292 23.66571884995145 32.2569755017802 10-11 23.84339097961556 25.84529125351951 30.48477866681516 19.82653910004977 12-13 28.486706783560194 24.13278250912723 28.922447246517034 18.45806346079554 14-15 20.776328016510806 29.83917278919141 28.197489271805292 21.187009922492493 16-17 18.277781064779848 30.488433039707374 32.37930998479085 18.85447591072193 18-19 19.30013538581477 28.784973218667233 33.59273580276131 18.322155592756683 20-21 20.91773517659998 27.4377340628437 30.935798911303237 20.708731849253088 22-23 18.686929329525817 30.327693408021883 31.70243607881626 19.282941183636037 24-25 20.8370602136242 27.747131317279617 33.61135570273591 17.804452766360278 26-27 18.479467644878483 28.797328479398036 34.217981602842755 18.505222272880726 28-29 17.449108507032058 31.09192662019232 31.0412874529717 20.41767741980392 30-31 19.40489407539145 27.612093538024617 32.669571603086375 20.313440783497548 32-33 18.30440578156595 29.271874902115012 34.085380072182566 18.338339244136474 34-35 19.351644641819252 28.82116891207579 33.817740762267384 18.00944568383758 36-37 18.366008067463206 27.86389723207356 34.12244585437498 19.647648846088256 38-39 19.40472005763468 29.84039091348881 32.42159629968642 18.333292729190088 40-41 21.327268234450646 27.598694170753184 33.10948849220575 17.96454910259043 42-43 17.9603726764279 30.554211751767152 32.386444712818495 19.09897085898645 44-45 18.749021150118157 29.375589485151067 31.43265338795171 20.44273597677907 46-47 21.260793451363778 28.55144138907934 31.071218507136468 19.116546652420414 48-49 20.385484134801114 28.962819366088116 30.837686677548575 19.81400982156219 50-51 17.666978738510476 30.963849551208206 31.986899943270213 19.382271767011105 52-53 18.485384248608728 30.3687088230483 31.44970712811535 19.696199800227614 54-55 18.49843558036662 28.411183077121187 31.86700170885437 21.223379633657817 56-57 20.15264837624031 28.463562421909533 29.513933601784725 21.86985560006543 58-59 21.506680541682474 29.344962359959208 28.555443797485097 20.59291330087322 60-61 19.299613332544453 27.614181751105882 33.68914164001295 19.397063276336716 62-63 19.357387227792724 27.845973403126056 32.669397585329605 20.127241783751614 64-65 19.883268888757407 30.44162226313573 29.65140762963453 20.023701218472333 66-67 20.37695726471929 30.519756235926316 28.560142276917936 20.543144222436457 68-69 20.67226539796121 28.043135521548617 31.520010301851205 19.764588778638974 70-71 22.522770223473604 27.966567708568984 30.204784096169174 19.30587797178824 72-73 20.861422699572266 28.999363095010217 30.940183136287224 19.199031069130292 74-75 18.91329391251083 31.344600403025126 31.475635773874366 18.266469910589677 76-77 19.98419918768511 27.95995503381165 32.040323394599184 20.015522383904052 78-79 19.75397369547589 28.119529316771484 31.559686350395193 20.566810637357435 80-81 20.82418289962308 28.882945215729812 31.065823956676542 19.22704792797057 82-83 19.954268133520344 28.35514935944064 32.98123740546485 18.709345101574165 84-85 19.082787207606664 29.0719284995841 32.72560532076693 19.119678972042305 86-87 17.911995740045313 31.386016629136837 29.77722246778061 20.924765163037236 88-89 18.918166409700447 29.347224590797243 30.545510863928556 21.189098135573754 90-91 19.935926327457885 28.528271942448548 31.624760071765294 19.911041658328273 92-93 19.40193577352633 30.643134825477592 31.617286227886694 18.337643173109385 94-95 18.9419976368183 30.63209019766849 31.070618760321516 19.35529340519169 96-97 19.505824374319154 29.676640204366457 31.757892575358387 19.059642845956002 98-99 20.344244921682627 29.21835764092516 31.76182330431272 18.675574133079497 100-101 18.86091456772249 28.726503252391872 32.16352796639369 20.249054213491945 102-103 19.21385601397713 29.475875006308154 31.237285805520898 20.07298317419382 104-105 21.049032096393596 28.481981887916692 30.829696298925946 19.63928971676377 106-107 19.615217277805815 28.20243295452568 31.967073319294826 20.21527644837368 108-109 18.409860542169724 29.742244898669462 31.220177706933217 20.6277168522276 110-111 18.011527377521613 31.050132954656195 30.96607916022776 19.972260507594424 112-113 19.525447090517993 28.190023041955854 30.160771592261803 22.123758275264354 114-115 18.92735201885026 29.186229843691812 29.02804219729428 22.858375940163654 116-117 20.314036269222672 28.978833962415184 28.15380572769022 22.553324040671928 118-119 19.89304850057339 27.289512105566317 31.328819304867462 21.488620088992835 120-121 18.536468221581448 28.90101992339698 30.985415495666064 21.577096359355505 122-123 19.99582354476638 28.604889933002696 29.5719133385539 21.82737318367702 124-125 20.998557380244286 29.012840881999274 28.8772798699728 21.111321867783637 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 134.0 1 85.0 2 154.5 3 214.0 4 89.5 5 35.0 6 111.5 7 291.5 8 463.5 9 464.5 10 293.0 11 139.0 12 117.5 13 152.5 14 183.0 15 229.0 16 271.0 17 320.0 18 405.0 19 512.0 20 625.5 21 746.0 22 895.0 23 1088.5 24 1300.5 25 1589.0 26 1997.5 27 2496.0 28 2940.0 29 3463.5 30 4327.0 31 5539.5 32 7247.0 33 9718.5 34 13099.0 35 16411.5 36 18504.0 37 19023.5 38 17337.0 39 14310.0 40 11885.0 41 10230.5 42 8856.0 43 7827.0 44 7365.5 45 7130.5 46 7059.5 47 9456.5 48 15275.0 49 14427.5 50 8658.0 51 6370.5 52 5021.0 53 4161.0 54 3532.0 55 2945.0 56 2354.0 57 1936.5 58 1576.5 59 1181.0 60 814.5 61 577.0 62 432.0 63 306.5 64 209.5 65 148.5 66 92.0 67 64.5 68 47.5 69 32.0 70 28.0 71 18.0 72 12.5 73 11.5 74 8.5 75 4.5 76 4.5 77 4.0 78 2.0 79 0.5 80 0.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016009633623014892 2 0.0 3 0.0 4 0.0 5 0.11032725779338524 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.003828390648981822 22-23 1.74017756771901E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 5.220532703157031E-4 92-93 0.0 94-95 8.700887838595051E-4 96-97 0.0 98-99 8.700887838595051E-4 100-101 0.0 102-103 1.74017756771901E-4 104-105 0.0017401775677190101 106-107 0.007656781297963644 108-109 0.0 110-111 0.003828390648981822 112-113 0.00870088783859505 114-115 0.0034803551354380203 116-117 0.001218124297403307 118-119 1.74017756771901E-4 120-121 5.220532703157031E-4 122-123 6.96071027087604E-4 124-125 8.700887838595051E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 287327.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.48488212684454 #Duplication Level Percentage of deduplicated Percentage of total 1 90.54218037367956 67.4402363264102 2 6.912445310065863 10.297453482570303 3 1.2458934311024326 2.7840067607482384 4 0.42094430756047546 1.2541594852243283 5 0.20173379034891056 0.7513058797570087 6 0.14409843961117616 0.643989317346041 7 0.07935521542638792 0.4137534707029402 8 0.054690493388837406 0.325889196282122 9 0.050696336920778365 0.3398499611826276 >10 0.2844778132433752 4.016431384189169 >50 0.02754106492999151 1.4057257284332108 >100 0.028007862640669068 3.8023785718885676 >500 0.005134774817455995 2.6939309779620175 >1k 0.002800786264066907 3.830889457303241 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4488 1.5619833847845834 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1849 0.6435176645424899 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1263 0.43956885360582193 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1188 0.41346619009003677 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1121 0.39014781068260207 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1109 0.3859713845200764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 955 0.3323739154343309 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 841 0.2926978668903375 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 823 0.28643322764654905 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 736 0.25615413796823827 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 734 0.25545806694115064 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 673 0.23422790061497872 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 659 0.22935540342536553 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 636 0.22135058661385806 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 597 0.20777720158564977 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 584 0.20325273990958037 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 510 0.17749811190733902 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 423 0.14721902222902825 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 421 0.14652295120194064 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 382 0.13294956617373238 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 352 0.1225085007674183 No Hit CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 329 0.11450368395591086 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 322 0.11206743536110425 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 304 0.1058027961173158 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 292 0.10162636995479019 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.48035513543802E-4 0.0 18-19 0.0 0.0 0.0 3.48035513543802E-4 0.0 20-21 0.0 0.0 0.0 3.48035513543802E-4 0.0 22-23 0.0 0.0 0.0 8.700887838595051E-4 0.0 24-25 0.0 0.0 0.0 0.001566159810947109 0.0 26-27 0.0 0.0 0.0 0.0017401775677190101 0.0 28-29 0.0 0.0 0.0 0.0022622308380347133 0.0 30-31 0.0 0.0 0.0 0.004524461676069426 0.0 32-33 0.0 0.0 0.0 0.009919012135998356 0.0 34-35 0.0 0.0 0.0 0.014791509325611584 0.0 36-37 0.0 0.0 0.0 0.026624716786100854 0.0 38-39 0.0 0.0 0.0 0.040198101814309134 0.0 40-41 0.0 0.0 0.0 0.06438657000560337 0.0 42-43 0.0 0.0 0.0 0.08335450549374057 0.0 44-45 0.0 0.0 0.0 0.10545476060377201 0.0 46-47 0.0 0.0 0.0 0.1339936727143638 0.0 48-49 0.0 0.0 0.0 0.16462079790621836 0.0 50-51 0.0 0.0 0.0 0.1912455146923192 0.0 52-53 0.0 0.0 0.0 0.2222206753977176 0.0 54-55 0.0 0.0 0.0 0.2632888659958862 0.0 56-57 0.0 0.0 0.0 0.30540116313468624 0.0 58-59 0.0 0.0 0.0 0.3616088985720103 0.0 60-61 0.0 0.0 0.0 0.4244293087666665 0.0 62-63 0.0 0.0 0.0 0.4858575769071476 0.0 64-65 0.0 0.0 0.0 0.5446755786960502 0.0 66-67 0.0 0.0 0.0 0.6266379421356155 0.0 68-69 0.0 0.0 0.0 0.7263501167659148 0.0 70-71 0.0 0.0 0.0 0.8439861203437199 0.0 72-73 0.0 0.0 0.0 0.9363895491895993 0.0 74-75 0.0 0.0 0.0 1.0307071733599695 0.0 76-77 0.0 0.0 0.0 1.1518235320732129 0.0 78-79 0.0 0.0 0.0 1.3042630870053982 0.0 80-81 0.0 0.0 0.0 1.4704500447225635 0.0 82-83 0.0 0.0 0.0 1.6235856706818366 0.0 84-85 0.0 0.0 0.0 1.7819418293442664 0.0 86-87 0.0 0.0 0.0 1.9329892422222763 0.0 88-89 0.0 0.0 0.0 2.0958698625607757 0.0 90-91 0.0 0.0 0.0 2.2928579632265675 0.0 92-93 0.0 0.0 0.0 2.490890170432991 0.0 94-95 0.0 0.0 0.0 2.6884003243690984 0.0 96-97 0.0 0.0 0.0 2.882952176440084 0.0 98-99 0.0 0.0 0.0 3.1084791892164674 0.0 100-101 0.0 0.0 0.0 3.363067167373759 0.0 102-103 0.0 0.0 0.0 3.655765034264096 0.0 104-105 0.0 0.0 0.0 3.9058285507453183 0.0 106-107 0.0 0.0 0.0 4.164766972821907 0.0 108-109 0.0 0.0 0.0 4.451026182711684 0.0 110-111 0.0 0.0 0.0 4.753817079494792 0.0 112-113 0.0 0.0 0.0 5.128129274311151 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCCCG 55 8.520292E-4 43.27007 9 GTCTATA 75 8.134421E-5 39.698772 1 CTATAGC 60 0.0013028006 39.691864 3 TACCTGG 290 0.0 39.00752 2 CATGGGG 745 0.0 35.962425 4 GTGTAGC 200 1.8189894E-12 35.728897 1 ACCTGGG 300 0.0 35.722675 3 TAGCCCT 240 0.0 34.73038 4 CTAGACA 105 1.4734056E-5 34.021595 4 TACATGG 1600 0.0 33.86212 2 GTACCTG 335 0.0 33.773586 1 GTACATG 1640 0.0 33.768166 1 TGTAGCC 215 3.6379788E-12 33.230392 2 ACATGGG 1560 0.0 33.20377 3 GGGAGCC 90 2.3894107E-4 33.053524 8 GTAGCCC 200 5.0931703E-11 32.74579 3 TAGGACC 595 0.0 30.019054 4 CGTTAGG 40 0.0053616096 29.748173 58-59 GTCTTAC 145 4.0274863E-6 28.74739 1 ATGGGGG 275 1.8189894E-12 28.145136 5 >>END_MODULE