FastQCFastQC Report
Fri 27 May 2016
SRR939908_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939908_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807445
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT134531.6661196737858306No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT72020.8919492968561326No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT56380.6982518933178111No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC50300.6229526469295121No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41530.5143384379121798No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32390.4011418734402962No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31520.3903671457498653No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30400.37649623194149445No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26850.3325303890667476No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25290.3132101876908025No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA23460.29054610530748226No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA21310.2639189046931989No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16400.20310980933685888No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC14340.17759723572503391No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA14090.17450104960709398No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT13770.17053793137613088No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC13470.16682250803460297No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG12220.15134157744490337No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT11330.14031915486503727No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG11160.13821374830483812No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC10550.1306590541770647No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC10340.12805825783799515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC2200.01979.5728108-109
CCGCTCG1050.01951.8475108-109
CGTCGCA304.3329055E-61707.8666108-109
CCGCGAC150.00279706341707.8666108-109
CGCGCAT200.00379678421465.4365110-111
CCGCGCC2600.01379.4308108-109
CGGCTCG459.747231E-61302.6102110-111
CCGCGCG1050.01219.9047108-109
CTCGTCG250.00593180631172.3492110-111
CGCCTAT250.00593180631172.3492110-111
CGTAATC250.00593180631172.3492110-111
CTCCGCC1600.01099.0774110-111
CGTCGCG250.0077676131024.7201108-109
CCGCGCA502.0048132E-51024.7201108-109
GCGCGTT300.008540839976.9576110-111
CGTGACG300.008540839976.9576110-111
CCTCTCG951.2588134E-7808.98944108-109
CGGCGCG654.4026627E-5788.24615108-109
TTGTTCG1001.5452497E-7768.54004108-109
CGTCTTA805.471793E-5732.71826110-111
Produced by FastQC (version 0.11.5)