##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939908_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 807445 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50543132968809 33.0 31.0 34.0 28.0 34.0 2 31.64089442624575 34.0 31.0 34.0 28.0 34.0 3 31.82503204552632 34.0 31.0 34.0 29.0 34.0 4 34.96117754150438 37.0 35.0 37.0 32.0 37.0 5 35.0993516586269 37.0 35.0 37.0 32.0 37.0 6 35.10716890933748 37.0 36.0 37.0 32.0 37.0 7 35.071495891361025 37.0 37.0 37.0 32.0 37.0 8 35.00298720036659 37.0 37.0 37.0 32.0 37.0 9 36.597994909870025 39.0 38.0 39.0 33.0 39.0 10-11 36.6127847717182 39.0 38.0 39.0 33.0 39.0 12-13 36.54191492919023 39.0 38.0 39.0 33.0 39.0 14-15 37.99952504504951 41.0 39.0 41.0 33.0 41.0 16-17 38.04935196824552 41.0 39.0 41.0 33.0 41.0 18-19 38.082499736824175 41.0 39.0 41.0 33.0 41.0 20-21 38.114761996173115 41.0 39.0 41.0 33.0 41.0 22-23 38.1034627745543 41.0 39.0 41.0 33.0 41.0 24-25 38.105511830527156 41.0 39.0 41.0 33.0 41.0 26-27 38.035636482980266 41.0 39.0 41.0 33.0 41.0 28-29 37.99213135259987 41.0 38.5 41.0 33.0 41.0 30-31 37.91332722352607 40.0 38.0 41.0 33.0 41.0 32-33 37.77512833691459 40.0 38.0 41.0 32.0 41.0 34-35 37.633754621057776 40.0 38.0 41.0 32.0 41.0 36-37 37.80123228207494 40.0 38.0 41.0 32.5 41.0 38-39 37.74900209921419 41.0 38.0 41.0 32.0 41.0 40-41 37.71958585414487 41.0 38.0 41.0 32.0 41.0 42-43 37.6038064512134 40.5 38.0 41.0 32.0 41.0 44-45 37.46939543869861 40.0 38.0 41.0 31.0 41.0 46-47 37.32027073051415 40.0 38.0 41.0 31.0 41.0 48-49 37.16838732049861 40.0 38.0 41.0 30.5 41.0 50-51 37.01458241737827 40.0 37.0 41.0 30.0 41.0 52-53 36.913804036188225 40.0 37.0 41.0 30.0 41.0 54-55 36.70614654868133 40.0 36.5 41.0 30.0 41.0 56-57 36.42376198998075 40.0 36.0 41.0 30.0 41.0 58-59 36.188129841661045 40.0 35.0 41.0 30.0 41.0 60-61 35.857139495569356 39.0 35.0 41.0 28.5 41.0 62-63 35.65733022063422 39.0 35.0 41.0 29.0 41.0 64-65 35.355597594882624 39.0 35.0 41.0 28.0 41.0 66-67 34.963208020360526 38.0 35.0 40.0 28.0 41.0 68-69 34.500400646483655 37.0 34.0 40.0 26.5 41.0 70-71 34.01037903510456 36.5 34.0 39.5 26.0 41.0 72-73 33.61680238282484 36.0 34.0 39.0 26.0 41.0 74-75 33.25076940225031 35.5 34.0 39.0 26.0 40.5 76-77 32.81972332480851 35.0 33.0 37.5 25.0 39.5 78-79 32.31690208001783 35.0 33.0 37.0 22.5 39.0 80-81 31.804247967353813 35.0 32.5 36.5 20.0 39.0 82-83 31.57782078036275 35.0 33.0 36.0 18.0 38.5 84-85 31.17569060431361 35.0 33.0 36.0 2.0 37.0 86-87 30.528708456922764 35.0 33.0 36.0 2.0 37.0 88-89 15.489890952324927 18.0 16.0 18.5 2.0 19.0 90-91 2.742592993950052 2.0 2.0 2.0 2.0 2.0 92-93 2.288454321966202 2.0 2.0 2.0 2.0 2.0 94-95 2.0997275356216214 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13081343331528217 1101 2 0.22441360393972332 1101 3 0.1361946632547273 1101 4 0.23475281581929153 1101 5 0.23153166158640914 1101 6 0.23821593940048302 1101 7 0.1622998978281487 1101 8 0.21606087761437465 1101 9 0.36286132211051836 1101 10-11 0.29674156611220326 1101 12-13 0.25323521333350385 1101 14-15 0.3860066513165137 1101 16-17 0.37627484434621294 1101 18-19 0.3498160570017603 1101 20-21 0.3347137360474406 1101 22-23 0.31236612044157397 1101 24-25 0.2325192295402161 1101 26-27 0.3528368236075252 1101 28-29 0.2147446833389779 1101 30-31 0.1988565155428219 1101 32-33 0.2146641344344573 1101 34-35 0.31550107970745245 1101 36-37 0.31392601508354545 1101 38-39 0.21204289961131195 1101 40-41 0.18834187910256617 1101 42-43 0.31206827094573697 1101 44-45 0.31633903239190175 1101 46-47 0.21135120805985252 1101 48-49 0.23374715636436605 1101 50-51 0.1609146732377127 1101 52-53 0.03518440223790975 1101 54-55 0.13358066919903422 1101 56-57 0.26004362432929895 1101 58-59 0.16284213450893503 1101 60-61 0.13027787937203783 1101 62-63 0.05055038227688158 1101 64-65 0.08832135273245001 1101 66-67 -0.09084053321749508 1101 68-69 0.005849220226096463 1101 70-71 0.0874649232127922 1101 72-73 -0.005497192243822724 1101 74-75 -0.020827177219203463 1101 76-77 0.019428679860759246 1101 78-79 0.06812933358675366 1101 80-81 0.1426194873043407 1101 82-83 0.2697545377160928 1101 84-85 0.34968778166057035 1101 86-87 0.35578710978000316 1101 88-89 0.8329698012930589 1101 90-91 -0.1467260887796793 1101 92-93 -0.22676508045508337 1101 94-95 -0.05368923876289422 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.07314407061236139 1102 2 0.2044156116262954 1102 3 0.2690289430688715 1102 4 0.20268289096412673 1102 5 0.24907740815899615 1102 6 0.169052571076044 1102 7 0.3553174507450336 1102 8 0.16596336140947443 1102 9 0.22944051100513718 1102 10-11 0.28401139918740625 1102 12-13 0.2702876426342513 1102 14-15 0.24914552586479033 1102 16-17 0.23970985266376488 1102 18-19 0.18782299786104772 1102 20-21 0.2135677772336919 1102 22-23 0.1813724589948933 1102 24-25 0.22369544705871647 1102 26-27 0.1566829774536771 1102 28-29 0.21973880368544485 1102 30-31 0.19057194031194058 1102 32-33 0.12591434954372716 1102 34-35 0.029037906415418036 1102 36-37 0.060421167954537225 1102 38-39 0.05246408357276522 1102 40-41 -0.10535757021768433 1102 42-43 -0.019944291435955108 1102 44-45 0.02806965821648788 1102 46-47 -0.03134009578917585 1102 48-49 0.038970009573802145 1102 50-51 0.01812872130746257 1102 52-53 -0.14668905614561822 1102 54-55 -0.15112362725023587 1102 56-57 -0.18295958796921497 1102 58-59 -0.1309168283775506 1102 60-61 -0.17014445183888682 1102 62-63 -0.304198513495507 1102 64-65 -0.284093750806818 1102 66-67 -0.3012353304411519 1102 68-69 -0.31621023597765685 1102 70-71 -0.3967460559646341 1102 72-73 -0.30469985386259424 1102 74-75 -0.2325126300959255 1102 76-77 -0.2099774751326393 1102 78-79 -0.33106185553732104 1102 80-81 -0.1782224504000176 1102 82-83 -0.09817697149058446 1102 84-85 -0.09911764482941976 1102 86-87 -0.10887975765531976 1102 88-89 0.8605668872794059 1102 90-91 0.0698896686917827 1102 92-93 -0.06753043925735547 1102 94-95 0.06824330296830539 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.0404747986728502 1103 2 0.2712274089813782 1103 3 0.22752864572794707 1103 4 0.14060589382426514 1103 5 0.06485417592158171 1103 6 -0.08309748697623576 1103 7 0.02302693450455706 1103 8 0.05327823128742892 1103 9 0.045540106055121043 1103 10-11 -0.05608219240775725 1103 12-13 -0.042453415899430524 1103 14-15 0.2212549473243044 1103 16-17 0.0931416624788497 1103 18-19 0.12346638698120671 1103 20-21 0.1782277466500588 1103 22-23 0.1985123932967099 1103 24-25 0.18078278033542006 1103 26-27 0.2269361420106364 1103 28-29 0.20311426031615554 1103 30-31 0.22813813406494887 1103 32-33 0.3230704960053714 1103 34-35 0.3883832608174842 1103 36-37 0.16357439764332327 1103 38-39 0.17465244196768737 1103 40-41 0.17396343054690533 1103 42-43 0.1676346454994544 1103 44-45 0.21477568715767603 1103 46-47 0.12790968474490683 1103 48-49 0.09991073961656127 1103 50-51 0.14006646378190624 1103 52-53 0.1977506686016426 1103 54-55 0.18404047662086498 1103 56-57 0.04729343499683125 1103 58-59 0.10118466399070769 1103 60-61 0.0892901048399537 1103 62-63 0.022015274627854353 1103 64-65 0.05434428900854016 1103 66-67 0.09524082564867342 1103 68-69 0.1528794052309692 1103 70-71 0.18339157040526288 1103 72-73 0.2201167859018014 1103 74-75 0.17666281520750715 1103 76-77 0.023426299681169382 1103 78-79 0.24039483180161625 1103 80-81 0.38189245062793375 1103 82-83 0.3285341547235596 1103 84-85 0.2636064227943997 1103 86-87 0.2972238727357528 1103 88-89 0.7072097666722659 1103 90-91 -0.43623166056373197 1103 92-93 -0.30368815737816046 1103 94-95 -0.1062533413269966 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.36419060354543475 1104 2 -0.20254009744394352 1104 3 -0.15460964644208985 1104 4 -0.16164881006116616 1104 5 -0.28175517824766416 1104 6 -0.24570761080970982 1104 7 -0.2746833143993612 1104 8 -0.2791102017586553 1104 9 -0.19829433367054605 1104 10-11 -0.18656879887568323 1104 12-13 -0.08016215296288465 1104 14-15 -0.11936167742315007 1104 16-17 -0.260220464543238 1104 18-19 -0.18555380296093205 1104 20-21 -0.0474134111448592 1104 22-23 -0.34133456721676936 1104 24-25 -0.25930195145033963 1104 26-27 -0.2667697912759692 1104 28-29 -0.24667545641992206 1104 30-31 -0.20334078950464374 1104 32-33 -0.14493036986689134 1104 34-35 -0.06567740521695953 1104 36-37 -0.18638162154331184 1104 38-39 -0.3130030370795751 1104 40-41 -0.22597575223460353 1104 42-43 -0.15676577231827338 1104 44-45 -0.41921735837620844 1104 46-47 -0.17211436726239526 1104 48-49 -0.12822770373752945 1104 50-51 -0.06741852089352562 1104 52-53 -0.1931989046470335 1104 54-55 -0.523227009188453 1104 56-57 -0.45088582804966393 1104 58-59 -0.3611924989719597 1104 60-61 -0.3577291642539038 1104 62-63 -0.42120412091084347 1104 64-65 -0.61864083997893 1104 66-67 -0.4700548766012176 1104 68-69 -0.35493337394412094 1104 70-71 -0.29898792903811255 1104 72-73 -0.1971123541358608 1104 74-75 -0.17788541937515845 1104 76-77 -0.34114371914811414 1104 78-79 -0.2638029306745011 1104 80-81 -0.27012585638562925 1104 82-83 -0.2437675536561592 1104 84-85 -0.11398603999370849 1104 86-87 -0.06948711834438726 1104 88-89 0.2703754918746455 1104 90-91 -0.6268139253007403 1104 92-93 -0.30368815737816046 1104 94-95 -0.1062533413269966 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.30618320221436335 1105 2 -0.24616295343389893 1105 3 -0.14181925436799148 1105 4 -0.0721286754142838 1105 5 -0.050415133807824475 1105 6 -0.05671018853298193 1105 7 -0.03602622592584481 1105 8 -0.1497176524673307 1105 9 -0.15800048383686516 1105 10-11 -0.10388221923039964 1105 12-13 -0.08383560772264786 1105 14-15 -0.189932161297115 1105 16-17 -0.12343886221346168 1105 18-19 -0.03971333509486641 1105 20-21 -0.06443574256414308 1105 22-23 -0.35819894339977054 1105 24-25 -0.337219344843831 1105 26-27 -0.12550936158462633 1105 28-29 -0.14619402478798804 1105 30-31 -0.23084590645788694 1105 32-33 -0.21528383121033556 1105 34-35 -0.1939622768219209 1105 36-37 -0.27917368608461146 1105 38-39 -0.2046394400539171 1105 40-41 -0.16564846633031038 1105 42-43 -0.21383606107105635 1105 44-45 -0.34864853369590065 1105 46-47 -0.12225692518153153 1105 48-49 -0.22555633557970367 1105 50-51 -0.15300503921501019 1105 52-53 -0.20684013122917122 1105 54-55 -0.1815615371412136 1105 56-57 -0.12815042829017642 1105 58-59 -0.1155959989414228 1105 60-61 -0.21142379032474423 1105 62-63 -0.0852227144986557 1105 64-65 -0.041017455701719996 1105 66-67 0.05889407067358832 1105 68-69 -0.018619465122789336 1105 70-71 -0.31305855493904744 1105 72-73 -0.13480465468087033 1105 74-75 -0.12319275513490169 1105 76-77 0.04559109392048555 1105 78-79 -0.1781530311552686 1105 80-81 -0.26190687467923723 1105 82-83 -0.3249605271701199 1105 84-85 -0.47942742532749705 1105 86-87 -0.40730193374913526 1105 88-89 0.9609262512543975 1105 90-91 -0.7509995930531836 1105 92-93 -0.30368815737816046 1105 94-95 -0.1062533413269966 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.11728335447431348 1106 2 -0.1785084089257012 1106 3 0.0424442745973046 1106 4 -0.21708199268488215 1106 5 -0.1893445967999554 1106 6 -0.3258196028888989 1106 7 -0.3664296587914606 1106 8 -0.175538594502477 1106 9 -0.19422398905725657 1106 10-11 -0.2634547317963012 1106 12-13 -0.15326970275103946 1106 14-15 -0.16155258007456297 1106 16-17 -0.23352623214847767 1106 18-19 -0.24417650841360228 1106 20-21 -0.2378114662271642 1106 22-23 -0.22513949349896478 1106 24-25 -0.28885589223910557 1106 26-27 -0.42274305326580475 1106 28-29 -0.2681923921607421 1106 30-31 -0.16030198068115453 1106 32-33 -0.2804087258768817 1106 34-35 -0.4317648448826432 1106 36-37 -0.3519412264506343 1106 38-39 -0.36527401683252236 1106 40-41 -0.36437043149170734 1106 42-43 -0.7151916424095646 1106 44-45 -0.2812153623734801 1106 46-47 -0.5347463259519856 1106 48-49 -0.39920581090549234 1106 50-51 -0.44281712895811864 1106 52-53 -0.5018973160079128 1106 54-55 -0.4650312192269226 1106 56-57 -0.5034056498313717 1106 58-59 -0.5096340105621948 1106 60-61 -0.2329516737928472 1106 62-63 -0.2019176787198802 1106 64-65 -0.2291461209597685 1106 66-67 -0.2814398602355723 1106 68-69 -0.1689305405682191 1106 70-71 -0.11072445141150666 1106 72-73 -0.18503792008689146 1106 74-75 -0.32822583263725846 1106 76-77 -0.29240688868458875 1106 78-79 -0.37239778951918723 1106 80-81 -0.3721971700345499 1106 82-83 -0.36776593157478743 1106 84-85 -0.43163420633078076 1106 86-87 -0.358097460693088 1106 88-89 0.4678273626663856 1106 90-91 -0.35568835618980454 1106 92-93 -0.2919662495366082 1106 94-95 -0.1062533413269966 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.12293579569168145 1107 2 -0.13962728071971497 1107 3 -0.07998297354146544 1107 4 -0.030609771192786184 1107 5 0.03609594037749275 1107 6 -0.07020771026027717 1107 7 -0.10217274293314915 1107 8 -0.07310997444307787 1107 9 -0.02187742139111748 1107 10-11 -0.09700678175985189 1107 12-13 -0.13825742919006956 1107 14-15 -0.10550801957959521 1107 16-17 -0.11622669763986693 1107 18-19 -0.11872302133112811 1107 20-21 -0.21811030131268438 1107 22-23 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0.06829271685855787 2216 14-15 -0.014913538735804366 2216 16-17 0.04245167854493559 2216 18-19 7.175358547613087E-4 2216 20-21 -0.004640593924570169 2216 22-23 0.06196650268464765 2216 24-25 -0.04011764622409686 2216 26-27 -0.18596753075586037 2216 28-29 -0.007681357304420544 2216 30-31 -0.07184141219185136 2216 32-33 -0.01044362202591742 2216 34-35 -0.11127364413528795 2216 36-37 -0.18861068893869515 2216 38-39 -0.27474565715379384 2216 40-41 -0.2029894895548381 2216 42-43 -0.29493038585744813 2216 44-45 -0.26791582750799137 2216 46-47 -0.29832949868887226 2216 48-49 -0.192704799290091 2216 50-51 -0.22007875779481623 2216 52-53 -0.2079549360538948 2216 54-55 -0.2928910000404201 2216 56-57 -0.40070061362369813 2216 58-59 -0.37307067130223004 2216 60-61 -0.462382318565723 2216 62-63 -0.44951410453506213 2216 64-65 -0.39419871168754383 2216 66-67 -0.469693870827669 2216 68-69 -0.5238274101542117 2216 70-71 -0.4256512933827139 2216 72-73 -0.5564000076110602 2216 74-75 -0.4867226751709879 2216 76-77 -0.47852010491366315 2216 78-79 -0.4719450434305479 2216 80-81 -0.4951604288962166 2216 82-83 -0.44133385265298486 2216 84-85 -0.5520426701480652 2216 86-87 -0.5937735111645104 2216 88-89 -1.1581584172002888 2216 90-91 -0.7509995930531836 2216 92-93 -0.30368815737816046 2216 94-95 -0.1062533413269966 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11237.0 3 2201.0 4 1952.0 5 2674.0 6 2485.0 7 1613.0 8 1610.0 9 1776.0 10 2104.0 11 2705.0 12 3297.0 13 3483.0 14 3760.0 15 4004.0 16 4654.0 17 5539.0 18 6773.0 19 8354.0 20 8991.0 21 12054.0 22 14230.0 23 18698.0 24 29860.0 25 52755.0 26 99118.0 27 231960.0 28 259783.0 29 9403.0 30 372.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.52389946064438 19.035352253094636 13.930855971614164 22.50989231464682 2 13.647932170895457 21.95974690410816 40.55825750735967 23.834063417636706 3 21.789610885203327 25.390465287431514 28.08574749044125 24.734176336923905 4 11.521434492192292 17.092209390065754 39.49363575426193 31.89272036348002 5 12.432301159631107 40.278688138655156 34.05033287199204 13.238677829721695 6 29.583562967137077 37.946113976803375 17.972369635083503 14.497953420976042 7 27.79483432308083 33.4271684139477 20.86519824879713 17.91279901417434 8 24.47535126231508 35.04325372006762 20.129049037395735 20.35234598022156 9 24.874016186861027 19.937952430196486 21.521218163466244 33.666813219476246 10-11 24.715582581987515 26.93890567720202 27.44117209947488 20.90433964133558 12-13 27.397950779573186 24.789176509637382 26.635840426836292 21.17703228395314 14-15 21.933754001820557 27.882518313940885 26.514561363312673 23.669166320925882 16-17 20.83895809234595 30.168668461635633 27.805650436811565 21.186723009206855 18-19 21.15894927646177 30.466511392003703 27.633605466526873 20.74093386500765 20-21 22.957997930456656 28.04540857999726 26.28419127732686 22.712402212219224 22-23 21.458896585376728 29.082940011567437 26.909710826452525 22.54845257660331 24-25 23.126157199561582 28.360445603106093 27.191387648694338 21.322009548637986 26-27 21.73900389500214 28.06934218429738 28.76932794184124 21.42232597885924 28-29 20.688848018725967 30.790156483184404 26.64839894232972 21.87259655575991 30-31 21.687111815665464 28.395432506238816 26.768944014762614 23.148511663333107 32-33 21.374343134352944 29.164772804678528 27.435403309005206 22.02548075196332 34-35 22.88409736886104 28.682510883094203 27.210522078903203 21.22286966914155 36-37 22.048402118289317 27.78335091969226 28.38834643640921 21.779900525609204 38-39 21.650948361807927 29.36602493049062 27.52738576621318 21.455640941488273 40-41 22.374960523626996 28.962591879323053 27.292880629640408 21.369566967409543 42-43 20.83553678578727 29.866863996928583 26.327365950622024 22.970233266662127 44-45 21.563105305135714 28.33893124476744 26.764332882528073 23.333630567568772 46-47 23.322579246883688 28.963768430047864 26.684480057465215 21.02917226560323 48-49 22.35164296223639 28.670471264880288 26.9305982829769 22.04728748990642 50-51 20.224238322262398 29.59435602075437 28.657325673776956 21.524079983206278 52-53 21.651196056697362 30.076661568280194 26.445702184049686 21.82644019097276 54-55 20.599900796783885 28.39150221039875 27.767054500463505 23.241542492353858 56-57 22.317371461833314 27.87725479754039 26.744050678374375 23.06132306225192 58-59 23.82329446587693 28.026800587036888 26.01688040671501 22.13302454037117 60-61 21.67441745258191 28.37029147496114 28.891627293499866 21.063663778957082 62-63 21.078786219981684 28.639243935651304 28.355756959147037 21.926212885219975 64-65 20.77127282432415 30.47800808600467 26.729019765445177 22.021699324226 66-67 21.81808048845432 29.00290422257863 25.748317222844904 23.43069806612215 68-69 21.64963966496706 28.27394627236845 27.394689160720944 22.681724901943543 70-71 23.353169565728933 28.112193400169673 26.537411216863067 21.997225817238323 72-73 22.416325570162673 27.67061533602908 28.21839258401501 21.694666509793237 74-75 20.880307637052677 30.76587259813362 27.185690666237328 21.168129098576376 76-77 21.73194459065323 27.978376236152307 27.19033494541424 23.099344227780218 78-79 21.92932862628438 28.436177729284413 27.44043995756968 22.194053686861526 80-81 23.22901250239954 28.219631058462184 26.945302776040474 21.606053663097796 82-83 22.093571213163603 28.079785643117095 28.546632344135187 21.28001079958412 84-85 21.500363182856532 29.066641732032355 28.12211861000973 21.310876475101384 86-87 21.344513565764263 29.950324297747066 26.656011552604102 22.049150583884565 88-89 31.075958210500517 19.133897887084668 33.06831222555885 16.72183167685597 90-91 31.41244769505482 14.375187928245426 15.144958819567048 39.06740555713271 92-93 23.084866574782883 17.212641769482406 32.918230351538504 26.78426130419621 94-95 16.1260974643202 7.043418562984545 27.132834346377322 49.69764962631793 96-97 27.207484068142218 7.213804744381925 14.940286561540017 50.63842462593584 98-99 57.11512362600325 14.184409911650771 18.819373915857025 9.881092546488949 100-101 31.811594296581386 6.93328127565446 12.116080005067866 49.13904442269629 102-103 24.447537660943922 2.159245160724769 36.72390297807683 36.66931420025448 104-105 43.952431643897725 4.833471326906776 38.4282531984939 12.785843830701598 106-107 6.024563585186968 2.0652399195031124 6.823515253625369 85.08668124168454 108-109 18.29577787017211 10.309790958215018 19.818914977302395 51.57551619431048 110-111 41.16856618241822 2.6612094347558806 23.926517455592585 32.24370692723331 112-113 28.559203471780116 2.921632110076279 38.07210919459006 30.447055223553544 114-115 29.856696777071484 4.208486673357868 23.501914426972974 42.43290212259767 116-117 20.392342515607893 4.3855593691747785 20.401403970766633 54.82069414445069 118-119 20.676177447723276 4.340276607836398 15.378083871880749 59.605462072559575 120-121 26.46218894907497 4.453840375527332 28.86818825811482 40.21578241728288 122-123 34.91271949182947 1.6857282237519648 33.3859881423435 30.015564142075064 124-125 39.728973784718754 1.9616469035573596 18.913345597792397 39.396033713931494 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 105.0 1 240.0 2 565.0 3 919.0 4 1131.5 5 1127.5 6 933.5 7 708.0 8 555.5 9 448.5 10 360.5 11 304.5 12 273.0 13 245.5 14 250.5 15 248.0 16 256.5 17 301.5 18 394.0 19 526.0 20 733.0 21 1000.5 22 1405.5 23 2017.5 24 2742.5 25 3677.5 26 4921.5 27 6304.0 28 7732.5 29 9532.5 30 11668.0 31 13901.5 32 16530.0 33 19272.5 34 21792.5 35 23875.0 36 25498.5 37 26447.0 38 26763.0 39 27132.0 40 27593.5 41 27824.5 42 29216.5 43 33566.5 44 38338.5 45 39061.5 46 35992.5 47 33107.0 48 32388.0 49 32449.0 50 31592.0 51 29360.0 52 25422.0 53 21176.5 54 18035.5 55 15669.0 56 13664.5 57 11837.0 58 10107.5 59 8443.0 60 6881.0 61 5498.5 62 4351.5 63 3389.0 64 2589.0 65 1913.5 66 1391.5 67 1032.5 68 774.0 69 579.5 70 397.0 71 262.0 72 184.5 73 143.5 74 108.0 75 76.5 76 63.5 77 49.0 78 25.0 79 16.0 80 15.0 81 14.5 82 9.0 83 5.5 84 5.0 85 3.5 86 3.5 87 3.0 88 2.0 89 2.5 90 3.5 91 3.0 92 2.0 93 2.0 94 2.0 95 2.0 96 3.0 97 3.5 98 2.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 4.953897788703874E-4 3 0.04099350420152456 4 0.04433738520889968 5 0.046814334103251615 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.192372235879843E-4 12-13 0.0019815591154815496 14-15 0.0 16-17 3.715423341527906E-4 18-19 0.0072450755159794164 20-21 0.0025388726167107356 22-23 7.430846683055812E-4 24-25 0.0 26-27 0.0 28-29 0.0012384744471759686 30-31 0.0 32-33 0.0014861693366111624 34-35 0.0 36-37 1.2384744471759685E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.2384744471759685E-4 46-47 0.0 48-49 1.2384744471759685E-4 50-51 6.192372235879842E-5 52-53 0.0 54-55 0.001424245614252364 56-57 0.0 58-59 0.0 60-61 0.0 62-63 6.192372235879842E-5 64-65 6.192372235879842E-5 66-67 0.0 68-69 2.476948894351937E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.3346605651158906E-4 80-81 0.0 82-83 8.050083906643797E-4 84-85 6.192372235879842E-5 86-87 9.907795577407748E-4 88-89 18.89806736062518 90-91 26.272749227501563 92-93 40.334264253292794 94-95 44.52780065515298 96-97 48.85961272904037 98-99 93.11018087919301 100-101 52.10193883174705 102-103 53.377505588615946 104-105 74.87036268724185 106-107 81.47601384614433 108-109 12.574664528234122 110-111 38.75595241781174 112-113 52.24151490194379 114-115 86.67351955860771 116-117 66.51462328703504 118-119 72.43285920403247 120-121 80.46287982463201 122-123 76.00895417025309 124-125 50.452105096941594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 807445.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.25094558003426 #Duplication Level Percentage of deduplicated Percentage of total 1 75.5634773917736 41.74953577211033 2 13.922988363996245 15.385165448212135 3 4.883284004546055 8.094181763610777 4 2.1627511433196895 4.779761828908522 5 1.1258561355810752 3.1102308038968833 6 0.6403989067988427 2.122958708943378 7 0.4226367385730451 1.634575561011574 8 0.26814992765695816 1.1852429648211775 9 0.18596427600944793 0.9247231884265631 >10 0.750989478347041 6.928909956441881 >50 0.03911240952000657 1.4786626401500145 >100 0.02517527500020372 2.802531478032507 >500 0.004270805577529234 1.4372816087832296 >1k 0.004046026336606643 4.476151710340029 >5k 6.743377227677738E-4 2.2193227641023343 >10k+ 2.2477924092259127E-4 1.6707638022086568 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13453 1.6661196737858306 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7202 0.8919492968561326 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5638 0.6982518933178111 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5030 0.6229526469295121 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4153 0.5143384379121798 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3239 0.4011418734402962 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3152 0.3903671457498653 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3040 0.37649623194149445 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2685 0.3325303890667476 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2529 0.3132101876908025 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2346 0.29054610530748226 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2131 0.2639189046931989 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1640 0.20310980933685888 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1434 0.17759723572503391 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1409 0.17450104960709398 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1377 0.17053793137613088 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1347 0.16682250803460297 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1222 0.15134157744490337 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1133 0.14031915486503727 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1116 0.13821374830483812 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1055 0.1306590541770647 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1034 0.12805825783799515 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.192372235879844E-5 0.0 16-17 0.0 0.0 0.0 2.4769488943519375E-4 0.0 18-19 0.0 0.0 0.0 2.4769488943519375E-4 0.0 20-21 0.0 0.0 0.0 3.096186117939922E-4 0.0 22-23 0.0 0.0 0.0 6.192372235879844E-4 0.0 24-25 0.0 0.0 0.0 0.0011146270024583718 0.0 26-27 0.0 0.0 0.0 0.002291177727275542 0.0 28-29 1.2384744471759688E-4 0.0 0.0 0.003839270786245503 0.0 30-31 2.4769488943519375E-4 0.0 0.0 0.009040863464384572 0.0 32-33 2.4769488943519375E-4 0.0 0.0 0.018391345540563136 0.0 34-35 2.4769488943519375E-4 0.0 0.0 0.03028070023345243 0.0 36-37 2.4769488943519375E-4 0.0 0.0 0.047495495049198394 0.0 38-39 2.4769488943519375E-4 0.0 0.0 0.07455616171999331 0.0 40-41 2.4769488943519375E-4 0.0 0.0 0.10774727690430927 0.0 42-43 2.4769488943519375E-4 0.0 0.0 0.14155762931221322 0.0 44-45 3.715423341527906E-4 0.0 0.0 0.17988841345230944 0.0 46-47 3.715423341527906E-4 0.0 0.0 0.22961316250642458 0.0 48-49 3.715423341527906E-4 0.0 0.0 0.27871867433695174 0.0 50-51 3.715423341527906E-4 0.0 0.0 0.33147768578664805 0.0 52-53 3.715423341527906E-4 0.0 0.0 0.3832459176786035 0.0 54-55 3.715423341527906E-4 0.0 0.0 0.4420115301971032 0.0 56-57 4.953897788703875E-4 0.0 0.0 0.5157626835264322 0.0 58-59 4.953897788703875E-4 0.0 0.0 0.5921146331948306 0.0 60-61 4.953897788703875E-4 0.0 0.0 0.6751543448779793 0.0 62-63 4.953897788703875E-4 0.0 0.0 0.7636433441287023 0.0 64-65 4.953897788703875E-4 0.0 0.0 0.8459399711435454 0.0 66-67 4.953897788703875E-4 0.0 0.0 0.9414263510208125 0.0 68-69 4.953897788703875E-4 0.0 0.0 1.0679984395221966 0.0 70-71 4.953897788703875E-4 0.0 0.0 1.2041067812668356 0.0 72-73 4.953897788703875E-4 0.0 0.0 1.3426301481834675 0.0 74-75 4.953897788703875E-4 0.0 0.0 1.4796673457634886 0.0 76-77 4.953897788703875E-4 0.0 0.0 1.6218442122992898 0.0 78-79 4.953897788703875E-4 0.0 0.0 1.6619088606654322 0.0 80-81 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 82-83 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 84-85 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 86-87 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 88-89 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 90-91 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 92-93 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 94-95 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 96-97 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 98-99 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 100-101 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 102-103 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 104-105 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 106-107 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 108-109 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 110-111 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 112-113 4.953897788703875E-4 0.0 0.0 1.6627757927784554 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 220 0.0 1979.5728 108-109 CCGCTCG 105 0.0 1951.8475 108-109 CGTCGCA 30 4.3329055E-6 1707.8666 108-109 CCGCGAC 15 0.0027970634 1707.8666 108-109 CGCGCAT 20 0.0037967842 1465.4365 110-111 CCGCGCC 260 0.0 1379.4308 108-109 CGGCTCG 45 9.747231E-6 1302.6102 110-111 CCGCGCG 105 0.0 1219.9047 108-109 CTCGTCG 25 0.0059318063 1172.3492 110-111 CGCCTAT 25 0.0059318063 1172.3492 110-111 CGTAATC 25 0.0059318063 1172.3492 110-111 CTCCGCC 160 0.0 1099.0774 110-111 CGTCGCG 25 0.007767613 1024.7201 108-109 CCGCGCA 50 2.0048132E-5 1024.7201 108-109 GCGCGTT 30 0.008540839 976.9576 110-111 CGTGACG 30 0.008540839 976.9576 110-111 CCTCTCG 95 1.2588134E-7 808.98944 108-109 CGGCGCG 65 4.4026627E-5 788.24615 108-109 TTGTTCG 100 1.5452497E-7 768.54004 108-109 CGTCTTA 80 5.471793E-5 732.71826 110-111 >>END_MODULE