##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939908_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 807445 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.77297029519038 34.0 31.0 34.0 31.0 34.0 2 32.8497111258352 34.0 31.0 34.0 31.0 34.0 3 32.895862876109206 34.0 31.0 34.0 31.0 34.0 4 36.29641275876376 37.0 37.0 37.0 35.0 37.0 5 36.21533231365604 37.0 37.0 37.0 35.0 37.0 6 36.375369220194564 37.0 37.0 37.0 35.0 37.0 7 36.37006235718842 37.0 37.0 37.0 35.0 37.0 8 36.39295308039557 37.0 37.0 37.0 35.0 37.0 9 38.20840304912409 39.0 39.0 39.0 37.0 39.0 10-11 38.203257807033296 39.0 39.0 39.0 37.0 39.0 12-13 38.196042454904045 39.0 39.0 39.0 37.0 39.0 14-15 39.6545232182997 41.0 40.0 41.0 37.0 41.0 16-17 39.68818495377394 41.0 40.0 41.0 37.0 41.0 18-19 39.76655437831678 41.0 40.0 41.0 37.0 41.0 20-21 39.77751054251373 41.0 40.0 41.0 37.0 41.0 22-23 39.71910780300826 41.0 40.0 41.0 37.0 41.0 24-25 39.65963006768263 41.0 40.0 41.0 37.0 41.0 26-27 39.526396225129886 41.0 39.5 41.0 36.5 41.0 28-29 39.45339125265498 41.0 39.0 41.0 36.0 41.0 30-31 39.30879812247274 41.0 39.0 41.0 36.0 41.0 32-33 39.098934911975434 41.0 39.0 41.0 35.0 41.0 34-35 38.93524388658051 41.0 39.0 41.0 35.0 41.0 36-37 38.908224089566474 41.0 39.0 41.0 35.0 41.0 38-39 38.941721727176464 41.0 39.0 41.0 35.0 41.0 40-41 38.89854107710123 41.0 39.0 41.0 35.0 41.0 42-43 38.750526042021434 41.0 39.0 41.0 35.0 41.0 44-45 38.55299556006911 41.0 39.0 41.0 35.0 41.0 46-47 38.26242035061212 41.0 39.0 41.0 34.5 41.0 48-49 37.95023066586579 41.0 38.0 41.0 34.0 41.0 50-51 37.74460179950337 41.0 38.0 41.0 33.0 41.0 52-53 37.66191752998656 40.5 38.0 41.0 33.0 41.0 54-55 37.52516518152939 40.0 38.0 41.0 33.0 41.0 56-57 37.32749846738787 40.0 37.0 41.0 33.0 41.0 58-59 37.13100582702228 40.0 37.0 41.0 33.0 41.0 60-61 36.84510338165448 40.0 36.0 41.0 32.0 41.0 62-63 36.66185436778976 39.0 36.0 41.0 32.0 41.0 64-65 36.39633597334803 39.0 35.0 41.0 31.5 41.0 66-67 36.06548681334333 39.0 35.0 41.0 31.0 41.0 68-69 35.67996829505415 37.5 35.0 40.0 31.0 41.0 70-71 35.24887391710891 37.0 35.0 39.5 30.5 41.0 72-73 34.86929450303117 36.5 35.0 39.0 30.0 41.0 74-75 34.5549040491922 36.0 35.0 39.0 30.0 41.0 76-77 34.1859786115463 35.5 35.0 38.0 30.0 40.0 78-79 33.81488584361783 35.0 34.5 37.0 29.0 39.0 80-81 33.530909226015396 35.0 34.0 37.0 29.0 39.0 82-83 33.300725746026046 35.0 34.0 36.0 29.0 38.5 84-85 33.05905665401359 35.0 34.0 36.0 29.0 37.0 86-87 32.933980642644386 35.0 34.0 36.0 29.0 37.0 88-89 32.738642879700784 35.0 34.0 35.0 29.0 36.5 90-91 32.55879409743079 35.0 34.0 35.0 29.0 36.0 92-93 32.42820377858554 35.0 34.0 35.0 29.0 36.0 94-95 32.31095554495972 35.0 34.0 35.0 29.0 36.0 96-97 32.21518183901071 35.0 34.0 35.0 28.5 36.0 98-99 32.169436927592585 35.0 34.0 35.0 29.0 35.5 100-101 32.11336747394559 35.0 34.0 35.0 27.5 35.0 102-103 31.17473697898928 34.5 32.0 35.0 25.0 35.0 104-105 31.881951711881307 35.0 34.0 35.0 27.0 35.0 106-107 31.89130467090638 35.0 34.0 35.0 26.5 35.0 108-109 31.94359306206615 35.0 34.0 35.0 27.0 35.0 110-111 31.942848738923395 35.0 34.0 35.0 27.0 35.0 112-113 31.824536036510224 35.0 34.0 35.0 26.0 35.0 114-115 31.73964790171467 35.0 34.0 35.0 25.0 35.0 116-117 31.653563400603137 35.0 34.0 35.0 25.0 35.0 118-119 31.536185746397585 35.0 34.0 35.0 25.0 35.0 120-121 31.458062778269728 35.0 34.0 35.0 25.0 35.0 122-123 31.270937958622568 35.0 34.0 35.0 23.5 35.0 124-125 29.177402795236826 33.0 29.5 34.5 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10219448960684474 1101 2 0.1354276410423978 1101 3 0.16182315325580987 1101 4 0.07420807259290996 1101 5 0.003373194922943412 1101 6 0.03477962981632032 1101 7 0.10166292193444804 1101 8 0.12942093397594334 1101 9 0.13531798417194096 1101 10-11 0.16576795053227755 1101 12-13 0.13576372364424572 1101 14-15 0.22218145874479944 1101 16-17 0.2222859279955074 1101 18-19 0.2337471573793266 1101 20-21 0.2845806187764879 1101 22-23 0.2270395958936433 1101 24-25 0.2256673523644963 1101 26-27 0.20238410150497543 1101 28-29 0.26869664001392124 1101 30-31 0.31309126619004957 1101 32-33 0.33227392885911655 1101 34-35 0.42107675369230435 1101 36-37 0.29647146090331944 1101 38-39 0.2560245768465066 1101 40-41 0.2218540347909439 1101 42-43 0.29341660085593446 1101 44-45 0.31059437227072095 1101 46-47 0.4088516928094421 1101 48-49 0.49887620834616797 1101 50-51 0.48656065170649754 1101 52-53 0.3021081592956918 1101 54-55 0.40851024583402307 1101 56-57 0.39486504658214727 1101 58-59 0.4072615649556397 1101 60-61 0.4474761051198044 1101 62-63 0.3803176472309815 1101 64-65 0.4167701246408413 1101 66-67 0.49141097335250095 1101 68-69 0.4595344364652547 1101 70-71 0.47846136102454295 1101 72-73 0.5631743227129391 1101 74-75 0.5140872480025322 1101 76-77 0.397760168773182 1101 78-79 0.45509436798270286 1101 80-81 0.4746850915067853 1101 82-83 0.5014633077141042 1101 84-85 0.46531640787734574 1101 86-87 0.4737394474532266 1101 88-89 0.45221946915152245 1101 90-91 0.5900129295671164 1101 92-93 0.5916202622065185 1101 94-95 0.5681671595709474 1101 96-97 0.6279132468720476 1101 98-99 0.6848123984193322 1101 100-101 0.6068955544761678 1101 102-103 0.6925515213743907 1101 104-105 0.6688066267010413 1101 106-107 0.6947360361158559 1101 108-109 0.6391133884343638 1101 110-111 0.5307168492656302 1101 112-113 0.6149820633067122 1101 114-115 0.5744211830894734 1101 116-117 0.6640931553896579 1101 118-119 0.77412044232236 1101 120-121 0.6970171836415204 1101 122-123 0.7162936942181126 1101 124-125 0.8492843149589291 1102 1 0.03974253067837452 1102 2 0.14973671004945288 1102 3 0.11939069756832055 1102 4 0.09479403025940059 1102 5 -0.1685923302305028 1102 6 0.010192639625309141 1102 7 -0.05080079630107548 1102 8 -0.08010267068188881 1102 9 -0.044347018982996644 1102 10-11 0.05539466423616801 1102 12-13 0.07650685448805206 1102 14-15 0.08698097690002271 1102 16-17 0.14764874564020403 1102 18-19 0.035903986381796926 1102 20-21 0.020996468027902893 1102 22-23 0.10354894597681152 1102 24-25 0.17818197979523376 1102 26-27 0.1312410108149038 1102 28-29 0.0990033858408026 1102 30-31 0.16186442628870878 1102 32-33 0.0391846728504035 1102 34-35 0.03470320926506787 1102 36-37 0.20269027005836904 1102 38-39 0.20392009110108944 1102 40-41 0.09263502486722786 1102 42-43 0.012054385517210164 1102 44-45 0.08081464416884643 1102 46-47 0.31552940175225785 1102 48-49 0.36658472093723304 1102 50-51 0.4362645179372038 1102 52-53 0.46567366724040227 1102 54-55 0.22055493120085856 1102 56-57 0.24403042259316265 1102 58-59 0.23986711190682541 1102 60-61 0.17937107443088962 1102 62-63 0.1526151576996284 1102 64-65 0.17100849439935217 1102 66-67 0.17149113740917699 1102 68-69 0.16147974134680254 1102 70-71 0.1351734874514321 1102 72-73 0.21742201387338156 1102 74-75 0.21402558334354893 1102 76-77 0.28139322103039177 1102 78-79 0.29800364923091394 1102 80-81 0.20679158493877736 1102 82-83 0.3425541985533158 1102 84-85 0.4015752273576396 1102 86-87 0.3706891979264668 1102 88-89 0.34333115388736957 1102 90-91 0.4472793208939194 1102 92-93 0.4321783990733792 1102 94-95 0.4302039575976764 1102 96-97 0.3176281553788982 1102 98-99 0.380574745691753 1102 100-101 0.3853387369461956 1102 102-103 0.36889236919174806 1102 104-105 0.3346408204714777 1102 106-107 0.3390686237370346 1102 108-109 0.23388623303942424 1102 110-111 0.31224756687016963 1102 112-113 0.3269342516850742 1102 114-115 0.4136449254172341 1102 116-117 0.37564237239189424 1102 118-119 0.33304030694692344 1102 120-121 0.38074560101087584 1102 122-123 0.3918264481904643 1102 124-125 0.36452839228353895 1103 1 0.04037473411620596 1103 2 0.08145165921830966 1103 3 0.1558510662710617 1103 4 0.07946610569904067 1103 5 -0.06700438811287057 1103 6 0.051365153996783874 1103 7 0.012784852043722594 1103 8 0.09227931329002104 1103 9 0.17112886808408945 1103 10-11 0.16843483357890676 1103 12-13 0.10943028212093253 1103 14-15 0.18344403583016344 1103 16-17 0.19742927972112767 1103 18-19 0.17532997681024654 1103 20-21 0.1688389764525624 1103 22-23 0.13087493074163348 1103 24-25 0.1688189265287221 1103 26-27 0.08287744782110451 1103 28-29 0.12346781820281905 1103 30-31 0.05172049912307841 1103 32-33 0.03756982845881396 1103 34-35 0.038803682178723875 1103 36-37 0.11299207109481557 1103 38-39 0.04944121646568789 1103 40-41 0.025462170677556628 1103 42-43 0.07138912060060676 1103 44-45 0.04064954916134411 1103 46-47 0.04818416062293096 1103 48-49 -0.15677671819283034 1103 50-51 -0.10349668898375342 1103 52-53 -0.054161450138472844 1103 54-55 0.08403226249528473 1103 56-57 0.040629408802210776 1103 58-59 -0.022025463065567408 1103 60-61 -0.11411645140945836 1103 62-63 -0.11683695625274026 1103 64-65 -0.03026546986310308 1103 66-67 -0.010206466872531905 1103 68-69 0.044988293438095184 1103 70-71 0.11049100507950271 1103 72-73 0.02767178024837591 1103 74-75 0.005107479529094405 1103 76-77 0.016320160117977878 1103 78-79 -0.037110543842459265 1103 80-81 -0.045335464589719265 1103 82-83 -0.04471217643606451 1103 84-85 -0.19614091117491483 1103 86-87 -0.18142121466946293 1103 88-89 -0.10918916440900972 1103 90-91 -0.047101643623399525 1103 92-93 -0.07241197840799884 1103 94-95 -0.11391116997573647 1103 96-97 -0.15340234330430036 1103 98-99 -0.12494092842259619 1103 100-101 -0.1247283771477612 1103 102-103 -0.2729845599844758 1103 104-105 -0.15600674558069016 1103 106-107 -0.11398407640172437 1103 108-109 -0.02619008446404436 1103 110-111 -0.11353826271099976 1103 112-113 -0.04722717611771543 1103 114-115 -0.0254706269289251 1103 116-117 -0.17852287836561942 1103 118-119 -0.09588388527690128 1103 120-121 -0.015408435746124383 1103 122-123 -0.13462592062555245 1103 124-125 -0.12179109345825623 1104 1 0.03439471789828019 1104 2 0.03538588041431723 1104 3 0.01918137592348046 1104 4 -0.005847051436155937 1104 5 -0.03908344362490368 1104 6 0.025383488498356144 1104 7 -0.06471002831472106 1104 8 0.008099349299307335 1104 9 -0.08497072097018332 1104 10-11 0.02726604346359096 1104 12-13 0.026540192922354322 1104 14-15 -0.03582196562670248 1104 16-17 -0.007730219447360298 1104 18-19 0.03882246571196646 1104 20-21 0.16775526172311572 1104 22-23 0.08909728124122296 1104 24-25 0.1005211901662122 1104 26-27 0.1861141608050616 1104 28-29 0.12422992323450188 1104 30-31 -0.002137442849736715 1104 32-33 -0.017482467477087482 1104 34-35 0.006384668723342202 1104 36-37 0.10933582238332207 1104 38-39 0.15151999709762975 1104 40-41 0.17000751901934308 1104 42-43 0.13353882029412034 1104 44-45 0.07824867779147837 1104 46-47 0.06668668264769195 1104 48-49 0.009162547052703474 1104 50-51 0.07420228983673383 1104 52-53 0.08080467089123289 1104 54-55 -0.12459897212860938 1104 56-57 0.08326812016437657 1104 58-59 0.13142565638357695 1104 60-61 -0.03611544809714928 1104 62-63 0.044463363982558235 1104 64-65 0.040697572509664326 1104 66-67 0.0911304811338951 1104 68-69 0.08278327412777031 1104 70-71 0.027560027468489068 1104 72-73 0.07343003695888228 1104 74-75 -0.019635851192880693 1104 76-77 -0.03410040640986978 1104 78-79 -0.007025262296536994 1104 80-81 -0.10134709201725656 1104 82-83 0.03343176451969043 1104 84-85 0.03350524943752475 1104 86-87 0.060373958436265696 1104 88-89 0.17971049061649325 1104 90-91 0.16363482884048608 1104 92-93 0.10101570751400857 1104 94-95 0.10906055860766628 1104 96-97 0.11470366419992217 1104 98-99 0.15713345412801516 1104 100-101 0.0902553362072851 1104 102-103 0.16210663148324755 1104 104-105 0.3133547767052107 1104 106-107 0.2349188084647551 1104 108-109 0.35910211306478246 1104 110-111 0.32202228649940423 1104 112-113 0.32347060697441066 1104 114-115 0.392143001625616 1104 116-117 0.3897989100042025 1104 118-119 0.5537134154006758 1104 120-121 0.4885202878482069 1104 122-123 0.4802347838921044 1104 124-125 0.47520652882902326 1105 1 0.03831970641665805 1105 2 0.041822224454094226 1105 3 -0.030591115442078376 1105 4 -0.05531358749109927 1105 5 -0.05115839139348566 1105 6 0.08114035955113508 1105 7 0.057801513036366714 1105 8 0.13965017770151888 1105 9 0.16535580834179342 1105 10-11 0.14482986932524256 1105 12-13 0.16813577973268679 1105 14-15 0.1977848691892845 1105 16-17 0.14809726688752534 1105 18-19 0.14055028362010802 1105 20-21 0.14001288007671064 1105 22-23 0.24991694945558862 1105 24-25 0.15656510172190252 1105 26-27 0.23682708313621958 1105 28-29 0.2571519092760326 1105 30-31 0.24630026040149033 1105 32-33 0.22220663945197572 1105 34-35 0.32623108048477434 1105 36-37 0.3823657622010401 1105 38-39 0.33862859331360795 1105 40-41 0.32613067366416004 1105 42-43 0.2878829874983637 1105 44-45 0.2890283262547939 1105 46-47 0.4259781007671535 1105 48-49 0.4443488014960266 1105 50-51 0.4489663524985019 1105 52-53 0.4249426733282533 1105 54-55 0.4107723819592408 1105 56-57 0.4360850327899115 1105 58-59 0.35414054850944154 1105 60-61 0.44091434305681787 1105 62-63 0.3971170119521048 1105 64-65 0.35564438539774557 1105 66-67 0.33535283697651863 1105 68-69 0.28390176975678827 1105 70-71 0.30652125277636344 1105 72-73 0.25472448675699155 1105 74-75 0.27542718489258533 1105 76-77 0.2851078794705231 1105 78-79 0.28663575514756445 1105 80-81 0.18996417536776988 1105 82-83 0.2252382016601473 1105 84-85 0.131785594762178 1105 86-87 0.09339025304534943 1105 88-89 0.21919332658993085 1105 90-91 0.26896409250361586 1105 92-93 0.23062826588444807 1105 94-95 0.23695386050923872 1105 96-97 0.2103385911555833 1105 98-99 0.15700536437758927 1105 100-101 0.35530422268924156 1105 102-103 0.23329400355941488 1105 104-105 0.32167165083745886 1105 106-107 0.31367110810715815 1105 108-109 0.4017364214730499 1105 110-111 0.40416365510851904 1105 112-113 0.313003086022114 1105 114-115 0.4268964719950752 1105 116-117 0.34216976500660223 1105 118-119 0.3671689450285669 1105 120-121 0.23421965788556065 1105 122-123 0.3781591989931563 1105 124-125 0.2047426979234217 1106 1 1.6379776208452768E-4 1106 2 0.07655277836801844 1106 3 0.002720278227307915 1106 4 -0.004020893267423276 1106 5 -0.012936597862193366 1106 6 0.020756870037700992 1106 7 0.022682897198563978 1106 8 -0.02400719722098188 1106 9 0.031152267556748825 1106 10-11 0.057777499413283806 1106 12-13 0.10039624373352041 1106 14-15 0.0011867030289494096 1106 16-17 0.04578398298102826 1106 18-19 0.026793126051813942 1106 20-21 0.1057982047475079 1106 22-23 0.16029243395649928 1106 24-25 0.0828227946048159 1106 26-27 0.04664544511959434 1106 28-29 0.05765151429619664 1106 30-31 0.05445305270139045 1106 32-33 0.19319917535428743 1106 34-35 0.18377842371006636 1106 36-37 0.04784057144785692 1106 38-39 -0.03258689701152662 1106 40-41 0.030502267004244743 1106 42-43 -0.007869941578547923 1106 44-45 -0.09971385897526375 1106 46-47 -0.02195934022470425 1106 48-49 -0.04965863954195271 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-1.1100917286622476 2216 72-73 -1.1965955963611279 2216 74-75 -1.2883298379954766 2216 76-77 -1.1522371581983464 2216 78-79 -1.1250279898957203 2216 80-81 -1.1544238544512595 2216 82-83 -1.18640515255467 2216 84-85 -1.181576196521128 2216 86-87 -1.0291965401973826 2216 88-89 -1.041335190939492 2216 90-91 -1.1707587069277352 2216 92-93 -1.1087302386521927 2216 94-95 -1.079117043499025 2216 96-97 -0.974074149798696 2216 98-99 -1.0332375604828492 2216 100-101 -1.0727346320343898 2216 102-103 -1.0170897582043423 2216 104-105 -1.1471182745639723 2216 106-107 -1.2041606713813202 2216 108-109 -1.231145828501944 2216 110-111 -1.2781813283972099 2216 112-113 -1.2965246560186117 2216 114-115 -1.4161196373966227 2216 116-117 -1.6794043687177194 2216 118-119 -1.5003183887622846 2216 120-121 -1.6530977565828806 2216 122-123 -1.6619983074124356 2216 124-125 -1.8654383917497057 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62.0 3 10.0 4 12.0 5 54.0 6 106.0 7 222.0 8 437.0 9 682.0 10 1461.0 11 2114.0 12 2688.0 13 4317.0 14 7293.0 15 7924.0 16 3167.0 17 2282.0 18 2090.0 19 1840.0 20 1853.0 21 1878.0 22 1928.0 23 1918.0 24 2333.0 25 2889.0 26 3566.0 27 4481.0 28 5685.0 29 6993.0 30 8853.0 31 11491.0 32 14720.0 33 20053.0 34 28931.0 35 53286.0 36 113620.0 37 292556.0 38 192104.0 39 1546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.780286477300315 18.824907967557017 14.519077842353257 21.875727712789413 2 14.146474372867502 21.849785434302028 40.40993504201524 23.593805150815225 3 21.780554712704888 25.22202750651747 29.04730353150989 23.950114249267752 4 11.616271077286997 17.03050981800618 39.751685873341216 31.601533231365604 5 11.905133889822984 39.33456832373612 34.5403008615174 14.219996924923494 6 29.326084129569196 38.14699453213532 17.4613750781787 15.065546260116788 7 26.90065577221978 33.649474577215784 21.218658856021154 18.23121079454328 8 24.85110440958827 33.674120218714584 20.072203060270358 21.402572311426784 9 25.2567047910384 18.241366285010123 21.952950355751785 34.548978568199686 10-11 24.819585234907642 26.127476174847825 27.481128745611155 21.57180984463338 12-13 28.313507421558125 23.962746688628947 27.207921282564136 20.515824607248792 14-15 22.349695644904607 26.514127897256163 28.290719491730087 22.845456966109147 16-17 19.998538598342424 28.991422315355614 29.43485864035153 21.57518044595043 18-19 20.239273263194395 28.25232678386763 31.15041891398176 20.357981038956215 20-21 22.01892982810319 25.749470834267395 30.28512403347523 21.946475304154184 22-23 20.58052395029742 26.718666209255638 30.9476334552408 21.75317638520614 24-25 22.28560459226325 26.055149267132744 31.12614481481711 20.533101325786895 26-27 20.89523125414115 25.7423106217761 32.88162041996669 20.48083770411607 28-29 19.823517391277424 28.53705205927339 30.72048250964462 20.91894803980457 30-31 20.83324560805999 26.044746081776466 30.83640371790029 22.28560459226325 32-33 20.605242462334896 26.73067515434488 31.529392094817606 21.13469028850262 34-35 22.03834316888457 26.377586089455008 31.11567970573847 20.46839103592195 36-37 21.277981782040882 25.483841004650472 32.37805670974493 20.86012050356371 38-39 20.935543597396727 27.081101499173315 31.320461455579018 20.662893447850937 40-41 21.629398906427063 26.81879261126145 30.885633077175534 20.666175405135952 42-43 20.326399940553227 27.642006576299316 29.788035098365835 22.243558384781625 44-45 21.091653301463257 26.135092792697957 30.022787929828038 22.75046597601075 46-47 22.8736322597824 26.941277734087148 29.64294781687917 20.54214218925128 48-49 22.2099338035408 26.876381673055132 29.42999213568726 21.48369238771681 50-51 20.11598313197803 27.90951705688932 30.840490683575972 21.134009127556673 52-53 21.501712190923218 28.484540742713126 28.492466979175052 21.5212800871886 54-55 20.515081522580488 26.683365430462757 29.776207667395305 23.025345379561458 56-57 22.182191975924056 26.287548997145315 28.763569035661874 22.766689991268755 58-59 23.731461585618835 26.296280241997906 28.092006266680702 21.880251905702554 60-61 21.641164413675234 26.713274588362058 30.870275994030553 20.775285003932158 62-63 20.987126058121603 26.99700908421007 30.363368402801427 21.652496454866895 64-65 20.67397779415316 28.77199066190267 28.955099108917636 21.598932435026537 66-67 21.63218547393321 27.288917511409444 27.99546718352334 23.08342983113401 68-69 21.498120615026412 26.75736427868152 29.49439280694041 22.250122299351656 70-71 23.191300955483037 26.52453108261244 28.597056146239062 21.687111815665464 72-73 22.183306602926514 26.1184972351058 30.34677284520927 21.351423316758417 74-75 20.763581420406343 29.178148356854027 29.127928218021044 20.93034200471859 76-77 21.548712296193546 26.56057068902526 29.114428846546826 22.77628816823437 78-79 21.750831325972666 26.991126330585985 29.255305314913087 22.00273702852826 80-81 22.904098731182927 26.845729430487523 28.704184185919786 21.545987652409764 82-83 21.93468285765594 26.501061991838455 30.376991621720368 21.18726352878524 84-85 21.232901312163676 27.59692610642211 29.787353937419887 21.382818643994327 86-87 21.020936410529508 28.470979447516548 28.293196440624442 22.214887701329502 88-89 20.985206422728485 27.984011294886958 27.663246413068382 23.367535869316175 90-91 21.758663524213034 26.736637037936084 28.503338340947966 23.00136109690292 92-93 22.37013047328301 27.86387927351089 28.887416480379468 20.878573772826634 94-95 21.549664092479993 27.47642393021397 29.063044351929772 21.910867625376266 96-97 20.41538432958282 27.318950516753464 30.44616041959514 21.819504734068573 98-99 21.67144932508165 27.96709564037051 28.189034109416163 22.172420925131682 100-101 20.693075928485445 26.845143440288123 29.221840771620077 23.239939859606363 102-103 21.763111158448535 26.281578119542953 29.015892163152273 22.93941855885624 104-105 22.95166733752361 26.366287890516148 28.276991671053036 22.405053100907203 106-107 21.565896551916328 26.756321755086294 29.832032950598254 21.845748742399124 108-109 20.755484604466943 27.259357328452072 29.154292196838906 22.83086587024207 110-111 20.333018125385884 28.903020608654778 28.49399339983044 22.269967866128894 112-113 21.39962829998334 26.90353821010526 27.78782753664205 23.909005953269343 114-115 20.38952192221947 27.414540500371565 28.028053009660642 24.167884567748327 116-117 22.08551954071532 27.093201325936196 27.41279579207844 23.408483341270042 118-119 21.401758390375544 26.26569305820968 29.454958430502217 22.87759012091256 120-121 20.221378678291885 28.757988209650254 28.34222976320222 22.678403348855642 122-123 20.82472069028296 27.348325627881803 27.78674992600042 24.04020375583482 124-125 21.09750418611103 27.465966174241295 27.94829781331431 23.488231826333365 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 496.0 1 334.5 2 387.5 3 534.0 4 279.5 5 141.0 6 457.5 7 1301.5 8 2192.5 9 2246.0 10 1456.5 11 718.5 12 537.5 13 626.0 14 695.5 15 767.0 16 872.5 17 965.0 18 1088.0 19 1215.5 20 1339.5 21 1554.5 22 1868.5 23 2252.5 24 2756.0 25 3467.0 26 4384.0 27 5454.0 28 6643.5 29 7953.5 30 9483.0 31 11879.0 32 15039.0 33 17494.5 34 19864.5 35 22542.0 36 24969.5 37 26364.0 38 26234.0 39 25467.0 40 24593.0 41 24351.0 42 24260.5 43 24216.5 44 24436.5 45 25321.5 46 26452.0 47 36569.0 48 57782.0 49 54492.0 50 34145.5 51 25512.0 52 21164.0 53 19601.5 54 19345.5 55 17978.0 56 15844.5 57 14138.0 58 12800.5 59 11330.5 60 9087.0 61 7267.5 62 6142.5 63 4546.5 64 3068.0 65 2384.0 66 1774.5 67 1268.0 68 972.0 69 656.0 70 439.0 71 336.0 72 272.0 73 270.0 74 203.0 75 122.5 76 89.0 77 49.0 78 32.0 79 18.5 80 8.5 81 7.0 82 7.5 83 5.0 84 2.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 2.0 98 3.5 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01498554081082922 2 0.0 3 0.0 4 0.0 5 0.11901739437361059 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.2384744471759685E-4 18-19 0.0 20-21 0.005201592678139068 22-23 2.476948894351937E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 9.907795577407748E-4 92-93 0.0 94-95 6.811609459467827E-4 96-97 0.0 98-99 0.0017338642260463562 100-101 1.2384744471759685E-4 102-103 3.715423341527906E-4 104-105 0.002476948894351937 106-107 0.006749685737109029 108-109 3.715423341527906E-4 110-111 0.00513966895578027 112-113 0.00910278718674337 114-115 0.005573135012291859 116-117 0.0019196353931227514 118-119 2.476948894351937E-4 120-121 6.192372235879843E-4 122-123 9.907795577407748E-4 124-125 0.0016100167813287594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 807445.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.189763927768674 #Duplication Level Percentage of deduplicated Percentage of total 1 73.44221481650615 38.32931853605931 2 15.087993503315273 15.748776381634633 3 5.277600497119033 8.263101721491509 4 2.4141854628686845 5.039830775398705 5 1.243087712559311 3.24382271299902 6 0.6759629259365404 2.11670073171311 7 0.4558973272549972 1.6655221719317306 8 0.28158077972349016 1.1756507536292788 9 0.21125845463072823 0.9922975987430643 >10 0.8218429001964823 7.220649825688841 >50 0.04656381692475038 1.676429673289897 >100 0.03159641928868983 3.342158231941438 >500 0.00451377418235842 1.4766695553665619 >1k 0.004751341244587811 5.758763294551696 >5k 7.127011866881716E-4 2.197398365219276 >10k+ 2.375670622293905E-4 1.7529096703419356 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14138 1.7509551734173845 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7141 0.8843946027283592 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5546 0.6868579284037922 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5036 0.6236957315978178 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4843 0.5997931747673216 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4289 0.5311816903937729 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3610 0.4470892754305246 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3351 0.4150127872486671 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3187 0.3947018063149812 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3135 0.38826173918966617 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2775 0.3436766590913313 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2586 0.3202694920397055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2553 0.31618252636402483 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2444 0.30268315488980674 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2337 0.28943147830502386 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1591 0.1970412845456966 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1494 0.1850280824080897 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1408 0.17437720216237637 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1345 0.16657481314516778 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1174 0.14539690009845874 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1165 0.14428227309600036 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1092 0.13524140963161577 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1058 0.13103059651121748 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1010 0.12508591916477282 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 982 0.12161819071268012 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 825 0.10217414189201743 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.4769488943519375E-4 0.0 0.0 0.0 0.0 14-15 2.4769488943519375E-4 0.0 0.0 6.192372235879844E-5 0.0 16-17 2.4769488943519375E-4 0.0 0.0 2.4769488943519375E-4 0.0 18-19 2.4769488943519375E-4 0.0 0.0 2.4769488943519375E-4 0.0 20-21 2.4769488943519375E-4 0.0 0.0 3.096186117939922E-4 0.0 22-23 2.4769488943519375E-4 0.0 0.0 6.811609459467828E-4 0.0 24-25 2.4769488943519375E-4 0.0 0.0 0.0012384744471759686 0.0 26-27 2.4769488943519375E-4 0.0 0.0 0.002415025171993139 0.0 28-29 3.715423341527906E-4 0.0 0.0 0.003963118230963099 0.0 30-31 3.715423341527906E-4 0.0 0.0 0.009164710909102168 0.0 32-33 3.715423341527906E-4 0.0 0.0 0.018453269262921936 0.0 34-35 3.715423341527906E-4 0.0 0.0 0.03034262395581123 0.0 36-37 3.715423341527906E-4 0.0 0.0 0.04774318993863359 0.0 38-39 3.715423341527906E-4 0.0 0.0 0.07480385660942851 0.0 40-41 3.715423341527906E-4 0.0 0.0 0.10762342945959168 0.0 42-43 3.715423341527906E-4 0.0 0.0 0.1411860869780604 0.0 44-45 3.715423341527906E-4 0.0 0.0 0.17951687111815665 0.0 46-47 4.3346605651158906E-4 0.0 0.0 0.2294273913393482 0.0 48-49 4.953897788703875E-4 0.0 0.0 0.27809943711336377 0.0 50-51 4.953897788703875E-4 0.0 0.0 0.330548829951266 0.0 52-53 4.953897788703875E-4 0.0 0.0 0.3823170618432215 0.0 54-55 4.953897788703875E-4 0.0 0.0 0.4407730557499272 0.0 56-57 4.953897788703875E-4 0.0 0.0 0.514586132801615 0.0 58-59 4.953897788703875E-4 0.0 0.0 0.591061929914731 0.0 60-61 4.953897788703875E-4 0.0 0.0 0.6736062518190093 0.0 62-63 4.953897788703875E-4 0.0 0.0 0.7622810222368087 0.0 64-65 4.953897788703875E-4 0.0 0.0 0.8444538018069342 0.0 66-67 4.953897788703875E-4 0.0 0.0 0.9408690375195834 0.0 68-69 4.953897788703875E-4 0.0 0.0 1.0676268971880438 0.0 70-71 4.953897788703875E-4 0.0 0.0 1.204540247323347 0.0 72-73 4.953897788703875E-4 0.0 0.0 1.3434970802964907 0.0 74-75 4.953897788703875E-4 0.0 0.0 1.4802865829870764 0.0 76-77 4.953897788703875E-4 0.0 0.0 1.622401525800519 0.0 78-79 4.953897788703875E-4 0.0 0.0 1.8120738873855187 0.0 80-81 4.953897788703875E-4 0.0 0.0 2.0178464167838057 0.0 82-83 4.953897788703875E-4 0.0 0.0 2.219903522840565 0.0 84-85 4.953897788703875E-4 0.0 0.0 2.4164494176073914 0.0 86-87 4.953897788703875E-4 0.0 0.0 2.623831963787007 0.0 88-89 4.953897788703875E-4 0.0 0.0 2.8531974314039967 0.0 90-91 7.430846683055812E-4 0.0 0.0 3.122999089721281 0.0 92-93 7.430846683055812E-4 0.0 0.0 3.4136071187511225 0.0 94-95 7.430846683055812E-4 0.0 0.0 3.67603985410771 0.0 96-97 7.430846683055812E-4 0.0 0.0 3.948566156208782 0.0 98-99 7.430846683055812E-4 0.0 0.0 4.243446922081381 0.0 100-101 8.669321130231781E-4 0.0 0.0 4.590653233347163 0.0 102-103 9.90779557740775E-4 0.0 0.0 4.99074240350736 0.0 104-105 9.90779557740775E-4 0.0 0.0 5.357454687316164 0.0 106-107 9.90779557740775E-4 0.0 0.0 5.717355361665501 0.0 108-109 9.90779557740775E-4 0.0 0.0 6.093789669884636 0.0 110-111 9.90779557740775E-4 0.0 0.0 6.519143718767222 0.0 112-113 9.90779557740775E-4 0.0 0.0 6.988959000303426 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 430 0.0 36.021458 5 TTTGCGT 105 1.4705796E-5 34.04226 3 GAGTACT 4215 0.0 29.146524 12-13 GTAAGGT 520 0.0 28.641321 4 GTCCTAC 1505 0.0 28.50226 1 AGGTAAG 455 0.0 27.49567 2 ACGCAGT 110 7.6931913E-4 27.079067 5 GTGTAGC 610 0.0 26.370434 1 GTACTTT 4660 0.0 26.3002 14-15 TACATGG 4520 0.0 26.096554 2 GTACATG 4570 0.0 26.073366 1 AGTACTT 4340 0.0 26.045229 12-13 TCCTACA 1695 0.0 26.008688 2 ACATGGG 4400 0.0 25.318928 3 AGTACGC 120 0.0012782427 24.82248 2 TAGGACC 745 0.0 24.78916 4 CATGGGG 1740 0.0 24.651287 4 TAGCCCT 615 0.0 24.21705 4 CCTACAG 1605 0.0 24.12652 3 GAGTACG 175 1.7323388E-5 23.831057 1 >>END_MODULE