FastQCFastQC Report
Fri 27 May 2016
SRR939907_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939907_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394132
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT81110.5817956979683416No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT41870.3003302413257855No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34750.2492590371643431No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27940.20041143880206463No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23920.17157629263226148No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21010.15070308980785177No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA19640.14087618675993377No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA19170.13750491345152396No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC18880.13542476609101578No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC18340.13155138824731086No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG16260.11663171062711421No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA15850.11369081263467161No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC15370.11024781010693391No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14270.10235759598086838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG108.8490226E-43038.412110-111
CCGCTCC2950.02519.3672108-109
CGTCGCG100.00133164112477.3777108-109
CCGCTCG800.02167.7053108-109
CCGCGCC1950.02032.7201108-109
GGTCGAC150.00199081282025.6082110-111
CGGATCG150.00199081282025.6082110-111
CGTCGCT150.00199081282025.6082110-111
CCGCACG457.3287083E-91651.5851108-109
TCGTTCG150.00299579111651.585108-109
CGTGTCG304.8145557E-61651.585108-109
GACGTCG200.00353883631519.206110-111
CCGTTCG850.01457.2809108-109
CCGCGCG900.01376.3209108-109
CGTCGCC701.8904757E-81302.1766110-111
CGACGCG200.00532513761238.6888108-109
CGCATCG250.00552882861215.3649110-111
CGCGTCT250.00552882861215.3649110-111
CGCGTCG250.00552882861215.3649110-111
CGCCGTC250.00552882861215.3649110-111
Produced by FastQC (version 0.11.5)