##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939907_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1394132 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.135002998281365 34.0 31.0 34.0 30.0 34.0 2 32.216036214648256 34.0 31.0 34.0 30.0 34.0 3 32.33897722740745 34.0 31.0 34.0 30.0 34.0 4 35.60587161043574 37.0 35.0 37.0 35.0 37.0 5 35.66114040851225 37.0 35.0 37.0 35.0 37.0 6 35.780261840342234 37.0 37.0 37.0 35.0 37.0 7 35.7248036771267 37.0 37.0 37.0 35.0 37.0 8 35.73030387366476 37.0 37.0 37.0 35.0 37.0 9 37.45210353108601 39.0 39.0 39.0 35.0 39.0 10-11 37.44705235946094 39.0 39.0 39.0 35.0 39.0 12-13 37.4185833909558 39.0 39.0 39.0 35.0 39.0 14-15 38.957221410885055 41.0 40.0 41.0 36.0 41.0 16-17 38.95290151865103 41.0 40.0 41.0 36.0 41.0 18-19 38.96797075169353 41.0 40.0 41.0 36.0 41.0 20-21 39.018696221017805 41.0 40.0 41.0 36.0 41.0 22-23 38.98184605188031 41.0 40.0 41.0 36.0 41.0 24-25 38.99407158002255 41.0 39.5 41.0 36.0 41.0 26-27 38.938652509231545 41.0 39.0 41.0 36.0 41.0 28-29 38.88768710566862 41.0 39.0 41.0 35.0 41.0 30-31 38.815878984199486 41.0 39.0 41.0 35.0 41.0 32-33 38.71722799562738 41.0 39.0 41.0 35.0 41.0 34-35 38.64533021263409 41.0 39.0 41.0 35.0 41.0 36-37 38.81369554676315 41.0 39.0 41.0 35.0 41.0 38-39 38.78982011746377 41.0 39.0 41.0 35.0 41.0 40-41 38.75014417573085 41.0 39.0 41.0 35.0 41.0 42-43 38.68375304490536 41.0 39.0 41.0 35.0 41.0 44-45 38.587693991673675 41.0 39.0 41.0 35.0 41.0 46-47 38.5012384049717 41.0 39.0 41.0 34.0 41.0 48-49 38.42139051395421 41.0 39.0 41.0 34.0 41.0 50-51 38.31982337397033 40.5 39.0 41.0 34.0 41.0 52-53 38.262643350844826 40.0 38.5 41.0 33.5 41.0 54-55 38.13761932155636 40.0 38.0 41.0 33.0 41.0 56-57 37.95865133287235 40.0 38.0 41.0 33.0 41.0 58-59 37.81199448832679 40.0 38.0 41.0 33.0 41.0 60-61 37.60277147357639 40.0 37.5 41.0 32.0 41.0 62-63 37.39401147093675 40.0 37.0 41.0 32.0 41.0 64-65 37.136073556879836 39.5 36.5 41.0 32.0 41.0 66-67 36.83351361277124 39.0 36.0 41.0 31.5 41.0 68-69 36.43617892710303 39.0 35.5 41.0 31.0 41.0 70-71 36.01204548780173 38.5 35.0 40.0 31.0 41.0 72-73 35.60976794162963 37.0 35.0 40.0 30.5 41.0 74-75 35.18509653318337 37.0 35.0 39.0 30.0 41.0 76-77 34.71314194064838 36.0 35.0 39.0 30.0 40.5 78-79 34.22736907265596 36.0 34.0 38.5 29.0 39.5 80-81 33.76056427942261 35.5 34.0 37.0 29.0 39.0 82-83 33.5225925522117 35.0 34.0 37.0 29.0 39.0 84-85 33.09153007032333 35.0 34.0 36.5 29.0 38.0 86-87 32.4697238138139 35.0 34.0 36.0 29.0 37.0 88-89 16.7052951227 18.0 17.0 18.5 2.0 19.0 90-91 2.8798245072919926 2.0 2.0 2.0 2.0 2.0 92-93 2.3267244421618614 2.0 2.0 2.0 2.0 2.0 94-95 2.107286110640886 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.08331106061193339 1101 2 0.17275098779854403 1101 3 0.24188673600974653 1101 4 0.19938822562329506 1101 5 0.15423727606435023 1101 6 0.1667590219615107 1101 7 0.2173338784314467 1101 8 0.17902761560066693 1101 9 0.22796866376384628 1101 10-11 0.3022100932474032 1101 12-13 0.2597370949485125 1101 14-15 0.21824391822094213 1101 16-17 0.3013901921393298 1101 18-19 0.28943833342746217 1101 20-21 0.3240025140006608 1101 22-23 0.33303033687144534 1101 24-25 0.2951711937954329 1101 26-27 0.2922035214041969 1101 28-29 0.31037996481080654 1101 30-31 0.20778117639825666 1101 32-33 0.31748470830214615 1101 34-35 0.3480774519111023 1101 36-37 0.22027624903309828 1101 38-39 0.23281666676041368 1101 40-41 0.13512203314711257 1101 42-43 0.2105695594380137 1101 44-45 0.14981109519628433 1101 46-47 0.17387520870418882 1101 48-49 0.17412029453926436 1101 50-51 0.12692892887764629 1101 52-53 0.09583491752756146 1101 54-55 0.048533063848140046 1101 56-57 0.1416382987445104 1101 58-59 0.12500144564675253 1101 60-61 0.05676300774153731 1101 62-63 0.038798932002137576 1101 64-65 0.06365910752498394 1101 66-67 0.16885985781023294 1101 68-69 0.14952773974906108 1101 70-71 0.09551032323872022 1101 72-73 0.14936270285738118 1101 74-75 0.12302025186617271 1101 76-77 0.10174365205760694 1101 78-79 0.07791548577282015 1101 80-81 0.15073562232691984 1101 82-83 0.21366147141219471 1101 84-85 0.18186260928731457 1101 86-87 0.32845961207421936 1101 88-89 0.5121892802604684 1101 90-91 -0.16389224778909028 1101 92-93 -0.20756442561487054 1101 94-95 -0.020835016753975477 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.08517875856525592 1102 2 0.01577153153625943 1102 3 0.015421202562905023 1102 4 0.029111761616206877 1102 5 0.01340403333076523 1102 6 0.01591794391202228 1102 7 0.09581980405111068 1102 8 -0.025220807294310532 1102 9 0.07823111739060806 1102 10-11 0.11810185984602839 1102 12-13 0.07298250100586046 1102 14-15 0.08778751508616978 1102 16-17 0.0031584135333275754 1102 18-19 0.016057172354678073 1102 20-21 0.04371068998946015 1102 22-23 0.05438832083341083 1102 24-25 0.06144206007716946 1102 26-27 0.1077440912560661 1102 28-29 0.12146530334412375 1102 30-31 0.07915372772814777 1102 32-33 0.059932840171100565 1102 34-35 0.05054405006115559 1102 36-37 0.046040754677143525 1102 38-39 0.11918557357049764 1102 40-41 0.05355923306335342 1102 42-43 0.08385113219959806 1102 44-45 0.007663174286385299 1102 46-47 -0.004451064989872577 1102 48-49 -0.004934481441935645 1102 50-51 0.044292187083705414 1102 52-53 -0.06745951950525608 1102 54-55 -0.022726269041385194 1102 56-57 -0.07167228557297278 1102 58-59 -0.1587955173783797 1102 60-61 -0.2125518760917373 1102 62-63 -0.18448039138647232 1102 64-65 -0.170234042973604 1102 66-67 -0.10479979706096998 1102 68-69 -0.14364723415553726 1102 70-71 -0.11245157345400969 1102 72-73 -0.142999283381819 1102 74-75 -0.20378137539452013 1102 76-77 -0.32433338254042354 1102 78-79 -0.23957132727804265 1102 80-81 -0.2013746644306451 1102 82-83 -0.12111712869587166 1102 84-85 -0.1607862523425041 1102 86-87 -0.05004787003657185 1102 88-89 0.7000247782582569 1102 90-91 -0.038137971436480456 1102 92-93 -0.07655317714323928 1102 94-95 0.0697386644038307 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.10939771302786028 1103 2 -0.04420132679901201 1103 3 -0.044337175709721066 1103 4 0.023117676207199622 1103 5 -0.03302070533134582 1103 6 -0.0638276016906758 1103 7 0.014968047198721024 1103 8 -0.003918884923187704 1103 9 -0.020166966897214422 1103 10-11 0.023673850556541254 1103 12-13 0.04319674361765635 1103 14-15 0.04246201900746627 1103 16-17 -0.05184475333835081 1103 18-19 -0.01300565043006685 1103 20-21 -0.016627507128816887 1103 22-23 0.06919325237788598 1103 24-25 -0.010905342907378213 1103 26-27 0.022880834555692786 1103 28-29 0.022329830922750205 1103 30-31 0.052252611038085206 1103 32-33 0.08208081025023262 1103 34-35 0.053250731670019036 1103 36-37 0.13372038495584349 1103 38-39 0.07853760116847752 1103 40-41 0.06250029001903101 1103 42-43 0.07391252356867994 1103 44-45 0.02592714530085516 1103 46-47 0.02802162945269515 1103 48-49 0.020508248251758232 1103 50-51 0.05594470047515898 1103 52-53 0.08484081142896827 1103 54-55 0.07249668440327639 1103 56-57 0.06666438669788022 1103 58-59 0.06010087300733602 1103 60-61 0.16136804515716818 1103 62-63 0.07521070054159651 1103 64-65 0.0015883613401541652 1103 66-67 0.02021872152446491 1103 68-69 0.06177485279999928 1103 70-71 0.07340236949481493 1103 72-73 0.14157910972632948 1103 74-75 0.19808341389624928 1103 76-77 0.07810941890472378 1103 78-79 0.06349480846228772 1103 80-81 0.033767106632666355 1103 82-83 0.033963958188934384 1103 84-85 0.041337332566563134 1103 86-87 0.05579855707659931 1103 88-89 0.8950325805835213 1103 90-91 -0.5969673232524375 1103 92-93 -0.3576425913261785 1103 94-95 -0.12245044145433992 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.16781092037344436 1104 2 -0.11855355596544825 1104 3 -0.04592858156888724 1104 4 -0.043935055119554534 1104 5 -0.08482315032986065 1104 6 -0.12298246657469747 1104 7 -0.026561529734458134 1104 8 -0.011964783314859062 1104 9 -0.04307547266821388 1104 10-11 -0.02126645237724034 1104 12-13 0.02019337757583628 1104 14-15 0.011599729160415961 1104 16-17 -0.020491311957499647 1104 18-19 -0.04138185228474356 1104 20-21 -0.005541586689624012 1104 22-23 -0.07655980337099777 1104 24-25 -0.008503630629562053 1104 26-27 -0.007371229154458092 1104 28-29 -0.022813423064121707 1104 30-31 0.029954830668572185 1104 32-33 -0.037196868728628374 1104 34-35 -0.03462632542488109 1104 36-37 -0.034027397560514316 1104 38-39 0.00585016975495023 1104 40-41 0.04146964316185375 1104 42-43 -0.03605711615201557 1104 44-45 -0.06430506764946387 1104 46-47 -0.07751703709148217 1104 48-49 -0.08638105380630634 1104 50-51 -0.10637370451429007 1104 52-53 -0.07433297558277019 1104 54-55 -0.1253287936675278 1104 56-57 -0.18201281178875206 1104 58-59 -0.22154900671981892 1104 60-61 -0.14666584592127663 1104 62-63 -0.08264178275051393 1104 64-65 -0.09065601269688273 1104 66-67 -0.08107345010672873 1104 68-69 -0.09857108263710757 1104 70-71 -0.0481283657321967 1104 72-73 -0.057094618871445846 1104 74-75 -0.07609809174594062 1104 76-77 -0.03184499324618173 1104 78-79 -0.022825042397393247 1104 80-81 0.018230367249778112 1104 82-83 -0.016863922343034687 1104 84-85 0.07408509511233774 1104 86-87 -0.04407420534435147 1104 88-89 0.582620263925957 1104 90-91 -0.7347861910601567 1104 92-93 -0.3576425913261785 1104 94-95 -0.12245044145433992 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.006902764264005157 1105 2 -0.12134298146968092 1105 3 -0.06164577766451629 1105 4 0.07065108162620248 1105 5 0.010604526211565712 1105 6 -0.0075759247485081005 1105 7 0.05142687173445637 1105 8 -0.025087164987944277 1105 9 0.16212008855927706 1105 10-11 -0.020511641118638124 1105 12-13 0.1559530553172479 1105 14-15 0.04012508358408695 1105 16-17 0.06215027182461341 1105 18-19 0.12092137830745031 1105 20-21 0.17446884079881642 1105 22-23 -0.09198279188339598 1105 24-25 0.06459561957517224 1105 26-27 0.03894560540344827 1105 28-29 -0.10160390192795887 1105 30-31 -0.10456953695393167 1105 32-33 -0.10284024765932287 1105 34-35 0.004554890047927529 1105 36-37 -0.11819379654649964 1105 38-39 -0.07421528902077057 1105 40-41 0.0371668572571906 1105 42-43 -0.0326844602871077 1105 44-45 -0.07135121869081473 1105 46-47 0.008461873530180242 1105 48-49 -0.0194582336192326 1105 50-51 -0.09783927306320805 1105 52-53 -0.0572547076840948 1105 54-55 -0.07625774205386904 1105 56-57 -0.0033477811091700005 1105 58-59 -0.15885361188842495 1105 60-61 -0.17667851613965269 1105 62-63 -0.1550969112710021 1105 64-65 -0.06576677461735869 1105 66-67 -0.05086303380781487 1105 68-69 -0.08276019830698544 1105 70-71 -0.005313204299049801 1105 72-73 -0.07624644734746511 1105 74-75 -0.15401619689477286 1105 76-77 -0.005714416203822736 1105 78-79 -0.0355275753799944 1105 80-81 -0.1568522037965323 1105 82-83 0.05908817680395373 1105 84-85 0.024174949002095047 1105 86-87 0.19037077446142803 1105 88-89 0.9025150630111156 1105 90-91 -0.8965655151158227 1105 92-93 -0.3576425913261785 1105 94-95 -0.12245044145433992 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.246255847576748 1106 2 -0.19502506452701596 1106 3 -0.1703841779950892 1106 4 -0.22075005669356074 1106 5 -0.2206295671782712 1106 6 -0.21176125367142618 1106 7 -0.2446483960780057 1106 8 -0.2412011104778884 1106 9 -0.23247804912578118 1106 10-11 -0.3048597097611321 1106 12-13 -0.319463639900853 1106 14-15 -0.3179784366521332 1106 16-17 -0.26220614421928445 1106 18-19 -0.29767623057042414 1106 20-21 -0.3055198909014436 1106 22-23 -0.3184406627700014 1106 24-25 -0.38215024358143523 1106 26-27 -0.39848141539791726 1106 28-29 -0.2523177570223396 1106 30-31 -0.20924844232305873 1106 32-33 -0.18092236643190063 1106 34-35 -0.3295259717693497 1106 36-37 -0.2812640206181669 1106 38-39 -0.32199398736604223 1106 40-41 -0.26647713645525783 1106 42-43 -0.5053619481207363 1106 44-45 -0.3257538499910808 1106 46-47 -0.39803650232575905 1106 48-49 -0.3363597834257348 1106 50-51 -0.32547138661001185 1106 52-53 -0.3497165750794622 1106 54-55 -0.45324397022586993 1106 56-57 -0.5807308223180598 1106 58-59 -0.4818032208548928 1106 60-61 -0.34017372399755885 1106 62-63 -0.34721553850415887 1106 64-65 -0.3284212897932264 1106 66-67 -0.36974761056480077 1106 68-69 -0.35482941944459867 1106 70-71 -0.4612017561079682 1106 72-73 -0.34696181134192017 1106 74-75 -0.4835540927820503 1106 76-77 -0.45813284388840714 1106 78-79 -0.3466032481037189 1106 80-81 -0.35586020581209254 1106 82-83 -0.378898798204375 1106 84-85 -0.4242331267972901 1106 86-87 -0.46300061360899036 1106 88-89 0.02949488874007855 1106 90-91 -0.33712661830174717 1106 92-93 -0.3369578552348802 1106 94-95 -0.12245044145433992 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.16922303730570576 1107 2 -0.24685578254888796 1107 3 -0.10286159121429961 1107 4 -0.14276622407349748 1107 5 -0.16784782680737464 1107 6 -0.18180404967713315 1107 7 -0.12957469184595283 1107 8 -0.15418256554207943 1107 9 -0.2353311161728584 1107 10-11 -0.2076176745733065 1107 12-13 -0.23339611731407928 1107 14-15 -0.2896855217686394 1107 16-17 -0.20966215943563782 1107 18-19 -0.16453310170689406 1107 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0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8711.0 3 1531.0 4 1461.0 5 1665.0 6 1947.0 7 1769.0 8 1648.0 9 1845.0 10 2103.0 11 2427.0 12 2964.0 13 3527.0 14 4060.0 15 4564.0 16 5229.0 17 6173.0 18 7873.0 19 10163.0 20 10612.0 21 15247.0 22 19285.0 23 24719.0 24 38439.0 25 68151.0 26 130227.0 27 400419.0 28 594413.0 29 22152.0 30 808.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.58482267102398 21.207604444916264 14.438015912410016 23.769556971649745 2 13.065857937827536 21.52824501729404 43.037481655120565 22.368415389757853 3 18.84015123844597 26.37215019651095 29.687110933787615 25.100587631255465 4 11.217249025813256 18.14262237436041 41.222774799601 29.41735380022534 5 11.955285372587209 39.5647808798106 36.46773963209538 12.012194115506807 6 28.66744325501459 39.238823870336525 19.458630890044844 12.635101984604042 7 26.665480743573777 32.48336599403787 23.54956345597117 17.301589806417184 8 23.11402363621235 38.11597467097807 21.69866268043485 17.071339012374725 9 24.832225355992115 18.056037735307704 22.87545225272786 34.23628465597231 10-11 23.15351393458674 27.304731798746307 30.471422494891048 19.07033177177591 12-13 25.21350600313032 25.40585149192386 29.025088479642154 20.35555402530367 14-15 20.701805854825796 29.14383286518063 26.780857192862655 23.373504087130918 16-17 19.94976067460138 30.627410558722413 29.511479226784935 19.911349539891273 18-19 20.327926239909672 29.925475221892167 30.047889827572234 19.69870871062593 20-21 21.384797746220237 29.106504005265037 28.631252006219054 20.87744624229567 22-23 20.464821520859118 30.46190571659028 28.495477275555697 20.577795486994905 24-25 21.28883778580507 29.505312265983424 29.192321817446263 20.013528130765234 26-27 20.062017083030874 30.107084551534573 29.56538548717051 20.26551287826404 28-29 19.845357312191158 30.317505632597552 28.6916736543633 21.14546340084799 30-31 20.8649898287967 29.191891442130302 28.95048675448236 20.99263197459064 32-33 20.081148051618282 30.14804695583625 29.444268905405103 20.32653608714037 34-35 20.644795933811096 29.983122102585767 29.409825905886894 19.96225605771624 36-37 20.34120179524019 29.227274911755064 29.54557355083561 20.885949742169135 38-39 20.677920024789618 30.31843469628414 28.835755868167436 20.16788941075881 40-41 21.492369445647903 29.18694212599668 29.487559284199776 19.83312914415565 42-43 20.021669397158952 30.344831048996795 29.072605750388053 20.5608938034562 44-45 20.247437114993417 29.834585247308006 28.780165723188333 21.137811914510245 46-47 21.486595243492008 29.106426077301144 28.90827410890791 20.49870457029894 48-49 20.703498916709076 29.465620710815298 28.95835148979849 20.872528882677134 50-51 19.51903389314423 30.199088106107634 29.55176753412429 20.730110466623845 52-53 20.036481480950155 30.269049128776903 28.953355923255476 20.74111346701747 54-55 20.05974450955202 29.356654422234225 29.370103930627796 21.21349713758596 56-57 20.759834793262044 29.071493947488474 28.383503140305223 21.785168118944263 58-59 21.30892196721688 29.24267572941443 28.18226681548089 21.266135487887805 60-61 20.45211644234549 29.12503980971673 29.82393345823781 20.59891028969997 62-63 20.289721489787194 29.111841633360395 29.853593490429887 20.74484338642252 64-65 20.31511363342926 29.948598841429646 28.67458031233771 21.06170721280338 66-67 20.91559479303251 29.828775180542443 27.828462441146172 21.42716758527887 68-69 21.079492370676284 28.830540965586003 29.216481275923474 20.87348538781424 70-71 21.864357177082226 28.89281646214275 28.67350437404779 20.569321986727225 72-73 21.280373737924386 29.03480445180227 28.915160113963385 20.769661696309964 74-75 20.266086712018662 30.34863269762117 29.08799166793388 20.297288922426286 76-77 20.63373482568365 28.84619246957964 29.439213790372794 21.08085891436392 78-79 20.651717808441244 28.812177621758643 29.204787785365937 21.331316784434172 80-81 21.12077622491988 29.382619436323104 28.720917388023516 20.7756869507335 82-83 20.664042802413267 29.212582497864254 29.64711966700308 20.4762550327194 84-85 20.419451106298077 29.123759128891358 29.562598650127335 20.894191114683228 86-87 20.002940933854095 30.171004544101454 28.52415331590292 21.30190120614153 88-89 30.270643252834223 18.512456729582315 35.30599667157464 15.91090334600882 90-91 31.691975403446083 16.076458889422433 18.104478975700893 34.127086731430595 92-93 23.09930890515458 18.099341954642885 36.55279415396615 22.248554986236392 94-95 17.445519661854906 7.526882418259212 30.003822498486247 45.023775421399634 96-97 29.87024645865737 7.7202139245835815 16.83399967291947 45.57553994383958 98-99 57.73323293433387 14.270506062887856 18.65292238984239 9.343338612935892 100-101 31.788338736468635 7.382931242998458 13.283987008703454 47.544743011829446 102-103 24.754122469989152 2.251962424398404 38.027755819867515 34.96615928574492 104-105 42.885856734260095 4.695972211325169 41.6647527478406 10.753418306574133 106-107 7.217569552967035 2.224253028998102 8.250098401031943 82.30807901700292 108-109 19.977182858345426 12.258326800377906 23.506537673102805 44.257952668173864 110-111 41.77814276778954 2.9026307497683868 27.15441428900625 28.16481219343582 112-113 28.65424356286596 2.9225419029106456 40.501158960960446 27.922055573262945 114-115 31.353764616215013 4.283354534925668 25.676529701667828 38.68635114719149 116-117 22.071976583092976 4.734985963988154 22.367685696115526 50.825351756803336 118-119 22.939762365647802 4.651998858781941 17.82577489601738 54.582463879552876 120-121 27.90701079992678 4.596558667398865 31.686252974556105 35.81017755811825 122-123 35.89361117581361 1.7910149134280577 35.938255899374674 26.37711801138366 124-125 40.357068998201946 2.1644157470544645 21.392616485808006 36.085898768935586 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34.0 1 99.5 2 230.5 3 344.0 4 395.0 5 382.0 6 359.0 7 336.0 8 291.0 9 262.0 10 247.5 11 215.5 12 188.5 13 177.5 14 179.5 15 193.5 16 226.5 17 308.5 18 453.0 19 628.5 20 933.5 21 1434.0 22 2169.0 23 3270.0 24 4787.0 25 6778.5 26 8993.0 27 11712.0 28 15126.5 29 19555.0 30 25586.0 31 32931.5 32 41062.5 33 49701.5 34 57715.0 35 63648.5 36 67071.0 37 67771.0 38 66949.5 39 65846.5 40 64124.0 41 62017.0 42 60600.0 43 60799.5 44 61186.0 45 59308.5 46 55230.0 47 50629.5 48 46662.5 49 43140.5 50 39269.0 51 34393.5 52 28781.0 53 23589.5 54 19118.0 55 15200.0 56 12095.5 57 9574.0 58 7453.0 59 5708.5 60 4386.5 61 3322.5 62 2384.0 63 1725.0 64 1274.5 65 948.0 66 684.0 67 499.5 68 372.5 69 271.5 70 204.0 71 146.5 72 117.0 73 90.0 74 57.0 75 36.0 76 29.0 77 23.0 78 13.5 79 7.5 80 8.5 81 7.0 82 6.0 83 5.5 84 4.5 85 3.0 86 1.5 87 4.0 88 4.5 89 4.0 90 7.0 91 6.5 92 3.0 93 2.5 94 3.0 95 2.5 96 2.5 97 4.5 98 4.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 7.172921932786852E-4 3 0.040957384236212924 4 0.04748474319504896 5 0.04827376460765552 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.890214126065537E-4 12-13 0.0018649597025245817 14-15 0.0 16-17 3.586460966393426E-4 18-19 0.007101192713458983 20-21 0.0021160119701721216 22-23 3.9451070630327687E-4 24-25 0.0 26-27 0.0 28-29 0.0010759382899180278 30-31 0.0 32-33 9.68344460926225E-4 34-35 7.172921932786852E-5 36-37 7.172921932786852E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 3.586460966393426E-5 50-51 3.586460966393426E-5 52-53 0.0 54-55 0.002080147360508187 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.2278148697540836E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 7.172921932786852E-5 80-81 0.0 82-83 3.586460966393426E-4 84-85 3.586460966393426E-5 86-87 0.0012193967285737649 88-89 19.228738742099026 90-91 26.207704865823324 92-93 40.31515667096085 94-95 44.73704785486597 96-97 48.46416982036134 98-99 93.0937314400645 100-101 51.72150126386884 102-103 53.177747874663226 104-105 74.85872930253376 106-107 81.59406713281095 108-109 12.574957034197624 110-111 38.99028212536546 112-113 52.06544287054597 114-115 86.77732811527173 116-117 66.50152209403414 118-119 71.96746792986603 120-121 80.40716374059271 122-123 75.98032324055399 124-125 50.05437074825052 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1394132.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.58028529631039 #Duplication Level Percentage of deduplicated Percentage of total 1 63.910448680341744 25.93504240863472 2 15.857452832291747 12.869999200143686 3 7.213281037272336 8.781510072449313 4 3.9697453438394517 6.443735944268189 5 2.4519668716245695 4.975075759381336 6 1.562153239142631 3.803557447257802 7 1.0613936462969438 3.0150159884894796 8 0.7425936310084036 2.4107729124435253 9 0.5675455422176439 2.072804401965705 >10 2.448626945470293 16.707649512130175 >50 0.13790735222500142 3.8537702871013932 >100 0.06999177596680385 5.0768603275396105 >500 0.0038884166834188293 1.1465100172498388 >1k 0.0028279394061227845 2.3259414044038174 >5k 1.7674621288267403E-4 0.5817543165413476 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8111 0.5817956979683416 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4187 0.3003302413257855 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3475 0.2492590371643431 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2794 0.20041143880206463 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2392 0.17157629263226148 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2101 0.15070308980785177 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1964 0.14087618675993377 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1917 0.13750491345152396 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1888 0.13542476609101578 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1834 0.13155138824731086 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1626 0.11663171062711421 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1585 0.11369081263467161 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1537 0.11024781010693391 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1427 0.10235759598086838 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.172921932786852E-5 0.0 10-11 0.0 0.0 0.0 7.172921932786852E-5 0.0 12-13 0.0 0.0 0.0 1.0759382899180279E-4 0.0 14-15 0.0 0.0 0.0 1.4345843865573704E-4 0.0 16-17 0.0 0.0 0.0 1.4345843865573704E-4 0.0 18-19 0.0 0.0 0.0 2.5105226764753985E-4 0.0 20-21 0.0 0.0 0.0 3.2278148697540836E-4 0.0 22-23 0.0 0.0 0.0 4.3037531596721115E-4 0.0 24-25 0.0 0.0 0.0 8.607506319344223E-4 0.0 26-27 0.0 0.0 0.0 0.0016856366542049102 0.0 28-29 0.0 0.0 0.0 0.004088565501688506 0.0 30-31 0.0 0.0 0.0 0.009324798512622908 0.0 32-33 0.0 0.0 0.0 0.02291748557525399 0.0 34-35 0.0 0.0 0.0 0.04185399947781128 0.0 36-37 0.0 0.0 0.0 0.06670817397491773 0.0 38-39 0.0 0.0 0.0 0.10669721375020444 0.0 40-41 0.0 0.0 0.0 0.15292669560701572 0.0 42-43 0.0 0.0 0.0 0.19524693501045814 0.0 44-45 0.0 0.0 0.0 0.2456725761979497 0.0 46-47 0.0 0.0 0.0 0.3085791015484904 0.0 48-49 0.0 0.0 0.0 0.37525141091374414 0.0 50-51 0.0 0.0 0.0 0.44325071083656353 0.0 52-53 0.0 0.0 0.0 0.5107837708337517 0.0 54-55 0.0 0.0 0.0 0.5862787741763333 0.0 56-57 0.0 0.0 0.0 0.6771238304550788 0.0 58-59 0.0 0.0 0.0 0.7775447375140948 0.0 60-61 0.0 0.0 0.0 0.880009927323955 0.0 62-63 0.0 0.0 0.0 0.9815785018922167 0.0 64-65 0.0 0.0 0.0 1.0909296967575524 0.0 66-67 0.0 0.0 0.0 1.2211540944472976 0.0 68-69 0.0 0.0 0.0 1.3778465740690264 0.0 70-71 0.0 0.0 0.0 1.5460515933928782 0.0 72-73 0.0 0.0 0.0 1.7095224842410905 0.0 74-75 0.0 0.0 0.0 1.8840396748657946 0.0 76-77 0.0 0.0 0.0 2.0732972200623756 0.0 78-79 0.0 0.0 0.0 2.1250498518074328 0.0 80-81 0.0 0.0 0.0 2.1258388732200393 0.0 82-83 0.0 0.0 0.0 2.1258388732200393 0.0 84-85 0.0 0.0 0.0 2.1258388732200393 0.0 86-87 0.0 0.0 0.0 2.1258388732200393 0.0 88-89 0.0 0.0 0.0 2.1258388732200393 0.0 90-91 0.0 0.0 0.0 2.1258388732200393 0.0 92-93 0.0 0.0 0.0 2.1258388732200393 0.0 94-95 0.0 0.0 0.0 2.1258388732200393 0.0 96-97 0.0 0.0 0.0 2.1258388732200393 0.0 98-99 0.0 0.0 0.0 2.1258388732200393 0.0 100-101 0.0 0.0 0.0 2.1258388732200393 0.0 102-103 0.0 0.0 0.0 2.1258388732200393 0.0 104-105 0.0 0.0 0.0 2.1258388732200393 0.0 106-107 0.0 0.0 0.0 2.1258388732200393 0.0 108-109 0.0 0.0 0.0 2.1258388732200393 0.0 110-111 0.0 0.0 0.0 2.1258388732200393 0.0 112-113 0.0 0.0 0.0 2.1258388732200393 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATTCG 10 8.8490226E-4 3038.412 110-111 CCGCTCC 295 0.0 2519.3672 108-109 CGTCGCG 10 0.0013316411 2477.3777 108-109 CCGCTCG 80 0.0 2167.7053 108-109 CCGCGCC 195 0.0 2032.7201 108-109 GGTCGAC 15 0.0019908128 2025.6082 110-111 CGGATCG 15 0.0019908128 2025.6082 110-111 CGTCGCT 15 0.0019908128 2025.6082 110-111 CCGCACG 45 7.3287083E-9 1651.5851 108-109 TCGTTCG 15 0.0029957911 1651.585 108-109 CGTGTCG 30 4.8145557E-6 1651.585 108-109 GACGTCG 20 0.0035388363 1519.206 110-111 CCGTTCG 85 0.0 1457.2809 108-109 CCGCGCG 90 0.0 1376.3209 108-109 CGTCGCC 70 1.8904757E-8 1302.1766 110-111 CGACGCG 20 0.0053251376 1238.6888 108-109 CGCATCG 25 0.0055288286 1215.3649 110-111 CGCGTCT 25 0.0055288286 1215.3649 110-111 CGCGTCG 25 0.0055288286 1215.3649 110-111 CGCCGTC 25 0.0055288286 1215.3649 110-111 >>END_MODULE