FastQCFastQC Report
Fri 27 May 2016
SRR939907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394132
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT80630.578352695440604No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT41890.30047369976444127No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35300.2532041442273759No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34200.24531393010131033No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28840.20686706854157283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25320.1816183833381631No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24080.1727239601415074No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21320.1529266956070157No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC19850.142382500365819No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19450.13951333159270426No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA19400.13915468549606494No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18140.1301168038607535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17080.12251350661199943No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA16680.11964433783888469No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG16230.11641652296913062No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC15510.11125201917752407No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15150.10866976728172081No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTA1254.886085E-528.557657
TAGGACC15650.027.0233754
GTAGGAC41150.026.4896853
GAGTACT38650.025.62984512-13
GTACTTT39500.025.1549814-15
TGTAGGA47800.024.9228462
AGTACTT40350.024.77119612-13
TAGGACG42450.024.1349434
TACAGTG40450.023.4139215
GTCCTAC37500.023.3522321
GGACGTG43700.023.144556
GACGTGG22950.023.0721767
CTACAGT40300.023.0576524
CGTGGAA23050.022.972089
AGGACGT45850.022.864885
CTGTAGG49600.022.6998211
CATGGGG19450.022.662494
GTACATG74450.022.4845371
ACGTGGA23600.022.4367128
AGTGGAC24000.022.0627678
Produced by FastQC (version 0.11.5)