FastQCFastQC Report
Fri 27 May 2016
SRR939906_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939906_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623164
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA35310.21753809226917306No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC28960.17841696834084542No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA21410.13190287611110152No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA18050.11120256486713603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT109.499266E-42933.2034110-111
CGTCGCT208.597217E-72933.2034110-111
CCGCTCC3250.02898.987108-109
CGTGTCG251.6789309E-62346.563110-111
CCGCGCC2300.02324.981108-109
CCTCGCG555.456968E-121851.9327108-109
CGACGCG150.00283628981697.605108-109
CAGCGCG651.4551915E-111567.0199108-109
CGCGTCG200.00379884661466.6017110-111
CATCGCG357.044562E-61455.09108-109
CCGGTCG551.46701495E-81388.9496108-109
CGTCGCC752.910383E-111358.084108-109
CTCCGCC1950.01353.7863110-111
CCGCACG200.00504163561273.2037108-109
CGTCGGC250.0059350261173.2815110-111
TTACGCG250.0059350261173.2815110-111
CGTCGCG451.5216665E-61131.7366108-109
CCGCGCA451.4967878E-51131.7366108-109
CGCCGCC1057.8216544E-111117.4109110-111
CAGCTCG1150.01107.1338108-109
Produced by FastQC (version 0.11.5)