##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939906_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1623164 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03274715309112 34.0 31.0 34.0 30.0 34.0 2 32.187851011974146 34.0 31.0 34.0 30.0 34.0 3 32.37641298106661 34.0 31.0 34.0 30.0 34.0 4 35.614056250631485 37.0 35.0 37.0 35.0 37.0 5 35.692041592839665 37.0 37.0 37.0 35.0 37.0 6 35.74878077631096 37.0 37.0 37.0 35.0 37.0 7 35.68512670315507 37.0 37.0 37.0 35.0 37.0 8 35.75379628922278 37.0 37.0 37.0 35.0 37.0 9 37.46476942564029 39.0 39.0 39.0 35.0 39.0 10-11 37.44346966788322 39.0 39.0 39.0 35.0 39.0 12-13 37.408251107097 39.0 39.0 39.0 35.0 39.0 14-15 38.981607218987115 41.0 40.0 41.0 36.0 41.0 16-17 38.9832731011777 41.0 40.0 41.0 36.0 41.0 18-19 38.99088693440712 41.0 40.0 41.0 36.0 41.0 20-21 39.03104461409937 41.0 40.0 41.0 36.0 41.0 22-23 39.02719102937226 41.0 40.0 41.0 36.0 41.0 24-25 39.04545381735918 41.0 40.0 41.0 36.0 41.0 26-27 39.00399220288276 41.0 40.0 41.0 36.0 41.0 28-29 38.96000927817522 41.0 39.5 41.0 36.0 41.0 30-31 38.881901335909376 41.0 39.0 41.0 35.0 41.0 32-33 38.79792707329635 41.0 39.0 41.0 35.0 41.0 34-35 38.76604921006134 41.0 39.0 41.0 35.0 41.0 36-37 38.939008627594006 41.0 39.5 41.0 35.0 41.0 38-39 38.9072610654253 41.0 40.0 41.0 35.0 41.0 40-41 38.828193577482004 41.0 39.0 41.0 35.0 41.0 42-43 38.80160137854216 41.0 39.0 41.0 35.0 41.0 44-45 38.73741162322476 41.0 39.0 41.0 35.0 41.0 46-47 38.659689039431626 41.0 39.0 41.0 35.0 41.0 48-49 38.598819959042956 41.0 39.0 41.0 34.0 41.0 50-51 38.50754575631298 41.0 39.0 41.0 34.0 41.0 52-53 38.43542519425024 41.0 39.0 41.0 34.0 41.0 54-55 38.328522564571415 41.0 39.0 41.0 33.5 41.0 56-57 38.17545916493958 40.0 38.0 41.0 33.0 41.0 58-59 38.049668117331336 40.0 38.0 41.0 33.0 41.0 60-61 37.879065516485085 40.0 38.0 41.0 33.0 41.0 62-63 37.667216313323856 40.0 37.5 41.0 33.0 41.0 64-65 37.42226293831061 40.0 37.0 41.0 32.0 41.0 66-67 37.14845234369417 39.5 36.5 41.0 32.0 41.0 68-69 36.767894371733234 39.0 36.0 41.0 31.0 41.0 70-71 36.37425115391913 39.0 35.5 41.0 31.0 41.0 72-73 35.98779082089056 38.0 35.0 40.0 31.0 41.0 74-75 35.561237804682705 37.0 35.0 39.5 31.0 41.0 76-77 35.07990350944205 37.0 35.0 39.0 30.0 41.0 78-79 34.59748737650662 36.0 35.0 39.0 30.0 40.0 80-81 34.136849079945094 36.0 34.5 37.5 29.5 39.0 82-83 33.884535388907096 35.5 35.0 37.0 30.0 39.0 84-85 33.4226609264375 35.0 35.0 37.0 30.0 39.0 86-87 32.785883619893 35.0 34.0 36.5 30.0 37.0 88-89 16.908900148105797 18.5 17.0 18.5 2.0 19.5 90-91 2.871485567693714 2.0 2.0 2.0 2.0 2.0 92-93 2.3356201221811226 2.0 2.0 2.0 2.0 2.0 94-95 2.113818443484454 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.04132652365700196 1101 2 0.024691512355126122 1101 3 0.049821846734865005 1101 4 0.04555428152567487 1101 5 0.06767175636882428 1101 6 0.052834257620112623 1101 7 0.05556448231467925 1101 8 0.10026908223900222 1101 9 0.16195929178143587 1101 10-11 0.08134959807284758 1101 12-13 0.12923142421851708 1101 14-15 0.07797183895582549 1101 16-17 0.241384534782199 1101 18-19 0.12366356835641312 1101 20-21 0.15602657971237477 1101 22-23 0.21510346754675425 1101 24-25 0.14658475499971502 1101 26-27 0.04111085520251123 1101 28-29 0.16028859776211846 1101 30-31 0.10450730564718214 1101 32-33 0.17514797294871443 1101 34-35 0.1789934990757942 1101 36-37 0.20909605778869178 1101 38-39 0.1955287803390533 1101 40-41 0.15406944022383584 1101 42-43 0.1318651683071579 1101 44-45 0.1038918393755992 1101 46-47 0.1784925730731004 1101 48-49 0.11542645891722714 1101 50-51 0.1951038889773713 1101 52-53 0.022368048474952218 1101 54-55 0.04639689305276562 1101 56-57 0.011763540035090614 1101 58-59 -0.030894622573256925 1101 60-61 0.03364569123330341 1101 62-63 0.031213319426335318 1101 64-65 0.06269282948208144 1101 66-67 0.11274611549011126 1101 68-69 0.12701954293868312 1101 70-71 0.12232044988503787 1101 72-73 0.12205124162735359 1101 74-75 0.14864881941883823 1101 76-77 0.1248941651824822 1101 78-79 0.09584073725305586 1101 80-81 0.22746203278813937 1101 82-83 0.20539547149757453 1101 84-85 0.20882791926787547 1101 86-87 0.27974410689169105 1101 88-89 0.5598599673113149 1101 90-91 -0.14516917060334844 1101 92-93 -0.274100483360856 1101 94-95 -0.06403913420531149 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.08430649562502524 1102 2 -0.0045241068458778955 1102 3 0.029586450760447747 1102 4 0.022989824622570154 1102 5 0.1426020548216158 1102 6 0.03729822926118942 1102 7 0.10489327382153135 1102 8 0.045047823342784454 1102 9 0.1855189444997194 1102 10-11 0.12125467985296723 1102 12-13 0.0377051949435625 1102 14-15 0.11364847372577458 1102 16-17 0.16672536096879043 1102 18-19 0.08253776349652497 1102 20-21 0.09580663765320452 1102 22-23 0.13432062305764703 1102 24-25 0.16673879466224406 1102 26-27 0.08834576228225188 1102 28-29 0.07483703617501192 1102 30-31 0.1071642514328488 1102 32-33 0.16198262967805022 1102 34-35 0.180045076707124 1102 36-37 0.1222890622053896 1102 38-39 0.18290303753700954 1102 40-41 0.21689259506267433 1102 42-43 0.16635683893442632 1102 44-45 0.10561394437745975 1102 46-47 0.12830670228053975 1102 48-49 0.07250665277985036 1102 50-51 0.06989784938748755 1102 52-53 0.13628769719731082 1102 54-55 0.16858064025638697 1102 56-57 0.16135944512595302 1102 58-59 0.17930227483756767 1102 60-61 0.12077016563368659 1102 62-63 0.1699275340011397 1102 64-65 0.10680876260492056 1102 66-67 0.17003533831407225 1102 68-69 0.11099102509176362 1102 70-71 0.13489775730140252 1102 72-73 0.23058812971436282 1102 74-75 0.19412302454875174 1102 76-77 0.14386626791393553 1102 78-79 0.12461930277856936 1102 80-81 0.1471796242174932 1102 82-83 0.11580968486150311 1102 84-85 0.12828630570354704 1102 86-87 0.3685167088103256 1102 88-89 0.9388555082286203 1102 90-91 0.01342663458953064 1102 92-93 -0.05081713938182908 1102 94-95 0.0826364576902372 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.033734960052370866 1103 2 0.1467275369836969 1103 3 0.18560141776718808 1103 4 0.1424157976949374 1103 5 0.07253891207832908 1103 6 0.033498508315730646 1103 7 0.09314137350532548 1103 8 0.14843089478753058 1103 9 0.0030829376411176668 1103 10-11 0.04085095129931915 1103 12-13 0.10581388037938666 1103 14-15 0.014438642139232627 1103 16-17 0.14809936368787646 1103 18-19 0.10237181828586017 1103 20-21 0.0977674752251616 1103 22-23 0.18333654929158172 1103 24-25 0.1751083344774642 1103 26-27 0.18469204001771544 1103 28-29 0.1719177735002546 1103 30-31 0.2705527078208263 1103 32-33 0.24157852560026072 1103 34-35 0.181133914416165 1103 36-37 0.21139118763556297 1103 38-39 0.21916089574420283 1103 40-41 0.20787728133413452 1103 42-43 0.2654352370724169 1103 44-45 0.25243659088929604 1103 46-47 0.2209163325423944 1103 48-49 0.19940874634515637 1103 50-51 0.15450279981648407 1103 52-53 0.09749350156089776 1103 54-55 0.1028892547436655 1103 56-57 0.06573404613155276 1103 58-59 0.12024353885182393 1103 60-61 0.19588985028091344 1103 62-63 0.1738451986930869 1103 64-65 0.17465075298494526 1103 66-67 0.06407090300211848 1103 68-69 0.1172562625305602 1103 70-71 0.0949675099987104 1103 72-73 0.1657368427421062 1103 74-75 0.23735360038038067 1103 76-77 0.1195520825442884 1103 78-79 0.16417885671422283 1103 80-81 0.16137033506281995 1103 82-83 0.17810467329135093 1103 84-85 0.17603374420964712 1103 86-87 0.15978635592336587 1103 88-89 0.9963862620149406 1103 90-91 -0.6055051414245396 1103 92-93 -0.35772417674064716 1103 94-95 -0.12283704361297731 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.11068074322825439 1104 2 0.07234993082792585 1104 3 0.10400598421788487 1104 4 0.06381005427710562 1104 5 0.01043145636291598 1104 6 0.13110597556731562 1104 7 0.06874141493145913 1104 8 0.056058074148296555 1104 9 0.1110306317684433 1104 10-11 0.08906382473085017 1104 12-13 0.1530163412444452 1104 14-15 0.1709301156935581 1104 16-17 0.19364122290597408 1104 18-19 0.17378020451066334 1104 20-21 0.11237660628770385 1104 22-23 0.01937249843319222 1104 24-25 0.11075973875924205 1104 26-27 0.1666017605352934 1104 28-29 0.2014028421370142 1104 30-31 0.16088759856633317 1104 32-33 0.08253296084217254 1104 34-35 0.1358455040365243 1104 36-37 0.07970005589888984 1104 38-39 0.17234919018964234 1104 40-41 0.1929026357591752 1104 42-43 0.19519564725359118 1104 44-45 0.10999678238628974 1104 46-47 0.15903352860371456 1104 48-49 0.16640679317576712 1104 50-51 0.11341797980592361 1104 52-53 0.11723458128071229 1104 54-55 0.13991620445653297 1104 56-57 0.10305347684486321 1104 58-59 0.1482710598220649 1104 60-61 0.1508391891368035 1104 62-63 0.14399236035233542 1104 64-65 0.03959444171854187 1104 66-67 0.11369101598027953 1104 68-69 0.13605146285836156 1104 70-71 0.1045592734839289 1104 72-73 0.17939489012437093 1104 74-75 0.04280889146749445 1104 76-77 0.024568617435477336 1104 78-79 0.09081357136396662 1104 80-81 0.08076623168968666 1104 82-83 0.15190524531201532 1104 84-85 0.2255334646526279 1104 86-87 0.2614588059999292 1104 88-89 0.9672301911346182 1104 90-91 -0.6968249610716648 1104 92-93 -0.35772417674064716 1104 94-95 -0.12283704361297731 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.2113587709283884 1105 2 -0.26342401248508196 1105 3 -0.11310763615504271 1105 4 -0.06697369687615407 1105 5 -0.04251411251919279 1105 6 -0.08345661392152692 1105 7 -0.024279242447072136 1105 8 -0.08407974209124092 1105 9 -0.049263220159865284 1105 10-11 -0.07025223754106946 1105 12-13 -0.0333793356402623 1105 14-15 -0.16497010441819526 1105 16-17 -0.048478197986568716 1105 18-19 -0.07643248120862722 1105 20-21 -0.07494450158075239 1105 22-23 -0.2421573603831888 1105 24-25 -0.12249968785511811 1105 26-27 -0.12747560094350519 1105 28-29 -0.10220721989945503 1105 30-31 -0.13004328045707325 1105 32-33 -0.1707263665302463 1105 34-35 -0.14430342387687745 1105 36-37 -0.23610016599237582 1105 38-39 -0.15351476213042758 1105 40-41 -0.1655174601153675 1105 42-43 -0.09578528050101909 1105 44-45 -0.21345678881739616 1105 46-47 -0.07960241504329701 1105 48-49 -0.10809501234290053 1105 50-51 -0.2622578919488632 1105 52-53 -0.18257374491177103 1105 54-55 -0.22643434814990115 1105 56-57 -0.19709595070031583 1105 58-59 -0.36286268681397615 1105 60-61 -0.3737587783884919 1105 62-63 -0.3134048763417212 1105 64-65 -0.27853334561079635 1105 66-67 -0.19992117804659415 1105 68-69 -0.17407853722376387 1105 70-71 -0.29052754951501214 1105 72-73 -0.1684958082376582 1105 74-75 -0.16151988745981072 1105 76-77 -0.0766887777992622 1105 78-79 -0.10450481043137216 1105 80-81 -0.25647922987425176 1105 82-83 -0.19879725065670328 1105 84-85 -0.1138690544497365 1105 86-87 -0.07369521747317975 1105 88-89 0.7316127420765248 1105 90-91 -0.8805785782688536 1105 92-93 -0.35772417674064716 1105 94-95 -0.12283704361297731 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.05440472604124835 1106 2 0.04172628214713825 1106 3 0.029556880250979134 1106 4 -0.04036287974133046 1106 5 -0.04022923697313274 1106 6 0.021000302153908024 1106 7 -0.13302013246178035 1106 8 -0.06089556743712876 1106 9 0.014491918462177011 1106 10-11 -0.05721214555570242 1106 12-13 -0.09606985374217203 1106 14-15 -0.021977548040936767 1106 16-17 -0.08971027318487046 1106 18-19 -0.008326955647000034 1106 20-21 -0.059041271359745906 1106 22-23 -0.11508849479773176 1106 24-25 -0.10085777065267365 1106 26-27 -0.16132174550113376 1106 28-29 -0.041023740233910644 1106 30-31 0.01914815803510095 1106 32-33 0.011875506987195195 1106 34-35 -0.05376137304342876 1106 36-37 -0.015087428113083945 1106 38-39 -0.024966027063292984 1106 40-41 0.06914583268143559 1106 42-43 -0.2492281572706858 1106 44-45 0.022102073226022867 1106 46-47 -0.14712833623856625 1106 48-49 0.05067499205780024 1106 50-51 -0.02940854615046362 1106 52-53 0.025190941082207985 1106 54-55 0.004049621496001521 1106 56-57 -0.09820397446409146 1106 58-59 -0.032366776244785456 1106 60-61 -0.03014328148508838 1106 62-63 0.0027193280439519185 1106 64-65 0.021596059114052935 1106 66-67 0.05178744732505436 1106 68-69 0.006382355581628474 1106 70-71 0.08024247903586712 1106 72-73 0.12992061066603355 1106 74-75 -0.0038202057055443106 1106 76-77 -0.057447120345443636 1106 78-79 -0.010473393728034353 1106 80-81 0.04443002447546718 1106 82-83 0.11328615870498027 1106 84-85 0.07998076630225626 1106 86-87 0.06701984258027238 1106 88-89 0.6255008677004064 1106 90-91 -0.27381196562703813 1106 92-93 -0.2983643712301123 1106 94-95 -0.12283704361297731 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.22520713497272382 1107 2 -0.14623042683501808 1107 3 -0.0982757645962451 1107 4 -0.14198479643722095 1107 5 -0.041758079542198345 1107 6 -0.08398949254949173 1107 7 -0.20716793977068448 1107 8 -0.14877888416828 1107 9 -0.03169168448574311 1107 10-11 -0.15650720908099203 1107 12-13 -0.13985121054887628 1107 14-15 -0.178191376494361 1107 16-17 -0.16482826684372043 1107 18-19 -0.12114859165656355 1107 20-21 -0.1635659394192217 1107 22-23 -0.16125884094499554 1107 24-25 -0.17775307023034514 1107 26-27 -0.10968016319542784 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114-115 0.0 2215 116-117 0.0 2215 118-119 0.0 2215 120-121 0.0 2215 122-123 0.0 2215 124-125 0.0 2216 1 0.332974071193739 2216 2 0.11303533831510748 2216 3 0.10830228136387632 2216 4 0.07420122849979549 2216 5 0.1213369244429785 2216 6 0.15959177568316818 2216 7 0.1713454733689872 2216 8 0.08909517857284754 2216 9 -0.06508475617848575 2216 10-11 0.031940823187717626 2216 12-13 -0.0276872638127017 2216 14-15 -0.030517380846418973 2216 16-17 -0.10304747820312343 2216 18-19 -0.033272008279425336 2216 20-21 -0.01995159587209372 2216 22-23 -0.05828593285509953 2216 24-25 -0.06219511923750787 2216 26-27 -0.12831880267464868 2216 28-29 -0.00705338067748329 2216 30-31 -0.027480780604314248 2216 32-33 -0.08682341372657731 2216 34-35 -0.05469102426890515 2216 36-37 -0.026128504873916825 2216 38-39 -0.033375338249321373 2216 40-41 -0.10821949870077674 2216 42-43 -0.13892160515747065 2216 44-45 -0.12706943520893788 2216 46-47 -0.027860765044820823 2216 48-49 -0.08937715604986352 2216 50-51 -0.04541052552414726 2216 52-53 0.025342147243229363 2216 54-55 -0.07852912246367083 2216 56-57 -0.0890941996560457 2216 58-59 -0.05112895273711615 2216 60-61 -0.014517587796191833 2216 62-63 -0.07027115793178496 2216 64-65 -0.09180366078698654 2216 66-67 -0.029576169906860628 2216 68-69 -0.07265962005587312 2216 70-71 -0.05396466446785553 2216 72-73 -0.05711175292987036 2216 74-75 -0.055428353137457975 2216 76-77 -0.0730189765690028 2216 78-79 -0.05017178572474279 2216 80-81 -0.05990330770472241 2216 82-83 -0.09142283934448159 2216 84-85 -0.11252860444372459 2216 86-87 -0.16685954823619653 2216 88-89 -1.2169049073659437 2216 90-91 -0.8805785782688536 2216 92-93 -0.35772417674064716 2216 94-95 -0.12283704361297731 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10775.0 3 1855.0 4 1204.0 5 1128.0 6 1231.0 7 1357.0 8 1575.0 9 1821.0 10 2247.0 11 2821.0 12 3283.0 13 4001.0 14 4588.0 15 5351.0 16 6298.0 17 7574.0 18 9559.0 19 12584.0 20 12849.0 21 18375.0 22 21931.0 23 27462.0 24 41593.0 25 71074.0 26 130590.0 27 417049.0 28 771532.0 29 30252.0 30 1205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.733345490658984 20.690269128689398 14.488184804492954 24.088200576158663 2 12.509965166583084 20.584306849504113 45.81857297582361 21.08715500808919 3 18.399299809234996 26.309051629823443 29.702696289173858 25.588952271767706 4 11.047976684781 17.78691576341562 41.33538557888612 29.829721972917262 5 11.867449195906207 39.54986281492312 36.544057953683165 12.038630035487508 6 28.694820732840302 38.77125170346311 20.340704944170767 12.193222619525814 7 27.291142484678073 32.212025402239085 23.328757907395676 17.168074205687166 8 22.884933377033988 40.07592578445555 21.64371560729538 15.395425231215084 9 25.55958609234803 17.51381868991673 22.380178466254797 34.54641675148044 10-11 23.181273507459238 27.185080907245457 31.177583163653498 18.456062421641803 12-13 25.396008829837115 24.90184106909292 29.911011605333638 19.791138495736334 14-15 19.894976692120046 29.699749901966126 26.74071342782166 23.664559978092164 16-17 19.57483563701803 30.66942548195326 30.361661708746606 19.394077172282103 18-19 20.492351218586517 28.907674288647 31.069011853470187 19.530962639296295 20-21 21.579576434347324 28.926854062379668 29.206037735849055 20.28753176742395 22-23 20.234888661074255 30.95809881021617 29.257518704348083 19.549493824361488 24-25 20.79333942842498 29.19473941018899 30.749727076253542 19.262194085132496 26-27 19.33122592664697 30.650168436461133 30.047703127964887 19.97090250892701 28-29 19.519900735825267 30.47983742657381 28.713696601686788 21.286565235914136 30-31 20.611626428383083 29.215501329502132 29.758145202826086 20.4147270392887 32-33 19.501707336232034 30.490083279810616 30.255415035956485 19.75279434800087 34-35 19.7520212079748 30.582233577280903 30.35730237813454 19.308442836609753 36-37 19.25155367993038 29.834782407799587 30.186534003835106 20.72712990843492 38-39 20.44888871364816 30.5638555315421 29.19590996350338 19.791345791306362 40-41 21.905580705338462 28.767641470609256 30.11882964383143 19.207948180220853 42-43 19.594970070799995 30.669020505629746 30.32965245656015 19.40635696701011 44-45 19.272383163233243 30.72521411910826 29.681756965724926 20.320645751933572 46-47 20.67988816903283 29.09478031794692 29.675282349781046 20.550049163239205 48-49 20.13554408001173 29.582105791042423 29.468808412222565 20.813541716723286 50-51 19.37488718015381 30.923480882696314 29.468266588136867 20.233365349013006 52-53 19.349893171608045 30.32290637298511 30.12656145651333 20.20063899889352 54-55 19.562114848203407 29.41815028563419 30.62100216093165 20.39873270523075 56-57 20.09039135909865 29.570856672523536 28.868925136338657 21.469826832039153 58-59 20.525559955740764 29.997923808068688 28.39694571836241 21.07957051782814 60-61 19.861702206308173 28.88888615075248 30.55578487447972 20.693626768459623 62-63 20.28868942921937 28.939114555962647 30.326313500246126 20.44588251457186 64-65 20.4908564327797 29.659867801323713 29.090300850869568 20.758974915027018 66-67 20.565019924049572 30.515123548821933 28.3594880123019 20.5603685148266 68-69 21.14479446214658 28.834104670161093 30.106748827592668 19.914352040099658 70-71 21.706648249961187 28.828879891372654 29.452815611977595 20.011656246688567 72-73 20.739555584032175 29.886936871443677 29.01770862340466 20.35579892111949 74-75 19.97256592679483 30.369266445041905 30.07163786284072 19.586529765322545 76-77 20.550018359204618 28.845175225670356 30.4259458686861 20.17886054643893 78-79 20.11450507482475 28.974170437347652 29.819897551657736 21.091426936169857 80-81 20.587938125783964 29.752477260461667 29.505552119194363 20.154032494560006 82-83 20.07476180648182 29.893879512431038 30.341495420954868 19.689863260132274 84-85 20.159096775862928 29.281994476217115 30.38034381020267 20.178564937717283 86-87 19.21025588579216 30.676353205441774 28.99086095767109 21.122529951094972 88-89 29.40397441801206 18.37371321617968 36.03265127649031 16.189661089317948 90-91 31.2629715477656 16.191501324064532 17.980482849863414 34.56504427830645 92-93 22.581344958407477 18.42508825982867 36.791913411103565 22.20165337066029 94-95 16.284823103117525 7.469901114996196 29.32976086229193 46.91551491959435 96-97 27.962931657716695 7.396943462325602 16.176006886621323 48.46411799333638 98-99 57.093215689248325 14.115059840573826 19.007132693071163 9.784591777106687 100-101 30.79514068938729 7.247367061524647 13.1822228660395 48.77526938304857 102-103 23.65136427885483 2.0993414734712212 38.03165248136327 36.217641766310685 104-105 41.333478846262324 4.662381392656829 42.46357476217219 11.540564998908653 106-107 6.233427290605331 1.9617409552898166 7.287564390249608 84.51726736385524 108-109 19.133502115296434 12.348023097493261 23.512868890391943 45.005605896818366 110-111 41.07775702085625 2.8934453163921856 26.68321611926105 29.345581543490518 112-113 27.555811660686715 2.79944202809539 40.182466331000306 29.46227998021759 114-115 29.743933959339873 4.035248409929405 25.1190367982772 41.10178083245352 116-117 20.738080045765585 4.481946797509296 21.605719231079526 53.1742539256456 118-119 21.176930211143993 4.362993246668157 16.865179059580278 57.594897482607564 120-121 26.32528502990439 4.4391022873051185 31.03713043464607 38.19848224814442 122-123 34.905419124848805 1.7774351027130146 35.32110443661918 27.996041335819005 124-125 39.42389601102148 2.100862430009917 20.958515390549458 37.51672616841914 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6.0 1 11.5 2 26.0 3 41.0 4 49.5 5 50.5 6 46.5 7 39.0 8 31.5 9 35.5 10 46.0 11 53.0 12 66.5 13 87.0 14 107.0 15 143.0 16 215.0 17 323.5 18 464.5 19 706.0 20 1115.5 21 1671.0 22 2551.0 23 3938.0 24 5735.5 25 8152.0 26 11453.0 27 15637.5 28 20890.5 29 27143.0 30 34610.0 31 43642.0 32 54017.0 33 64795.0 34 75046.0 35 83010.0 36 86565.5 37 86573.5 38 84827.5 39 82020.0 40 79035.0 41 75607.5 42 71318.5 43 67035.5 44 63201.0 45 59450.0 46 55422.5 47 51390.5 48 47368.5 49 42934.5 50 38394.0 51 33906.5 52 29154.0 53 24368.0 54 19986.5 55 16289.5 56 13020.5 57 10123.0 58 7814.0 59 5967.0 60 4442.5 61 3191.0 62 2228.5 63 1601.5 64 1139.0 65 766.5 66 549.5 67 407.0 68 293.0 69 216.0 70 156.0 71 102.5 72 76.0 73 62.0 74 42.5 75 29.0 76 20.5 77 17.0 78 13.0 79 9.0 80 4.5 81 4.5 82 6.5 83 6.5 84 4.0 85 2.0 86 2.5 87 2.5 88 2.5 89 3.5 90 5.5 91 5.5 92 3.0 93 1.0 94 4.0 95 6.0 96 5.5 97 3.0 98 2.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 6.160806917846872E-4 3 0.046144443814673065 4 0.053968668600338596 5 0.05563208646815725 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.544726226062185E-4 12-13 0.00190985014453253 14-15 3.0804034589234355E-5 16-17 2.1562824212464053E-4 18-19 0.005852766571954529 20-21 0.00240271469796028 22-23 7.392968301416247E-4 24-25 0.0 26-27 0.0 28-29 0.0012629654181586087 30-31 0.0 32-33 0.0013245734873370775 34-35 6.160806917846871E-5 36-37 9.241210376770308E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.2321613835693742E-4 46-47 0.0 48-49 1.2321613835693742E-4 50-51 6.160806917846871E-5 52-53 0.0 54-55 0.0013245734873370775 56-57 0.0 58-59 0.0 60-61 0.0 62-63 6.160806917846871E-5 64-65 6.160806917846871E-5 66-67 0.0 68-69 4.928645534277497E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.7723631130310925E-4 80-81 0.0 82-83 5.544726226062185E-4 84-85 6.160806917846871E-5 86-87 0.001108945245212437 88-89 19.125824624005954 90-91 26.308894233731156 92-93 40.362002853685766 94-95 44.665819350355235 96-97 48.61388005155363 98-99 93.09438232982004 100-101 51.94142427998649 102-103 53.23214413331 104-105 74.87912496827185 106-107 81.56381610237783 108-109 12.603070299735577 110-111 38.97311054212636 112-113 52.102282948611474 114-115 86.7829436828318 116-117 66.3996983668933 118-119 72.16689133075894 120-121 80.38205011939644 122-123 75.9087190203824 124-125 50.20592497122903 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1623164.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.55897437920945 #Duplication Level Percentage of deduplicated Percentage of total 1 79.14773584544777 54.26287594000492 2 13.295180988727594 18.230079455462555 3 3.976415693437648 8.178569449424344 4 1.5520264490375595 4.256213662216858 5 0.7334924106280104 2.514374369379517 6 0.39168092788408015 1.611194561977778 7 0.23345440295723138 1.1203776101740925 8 0.15139277144440902 0.83034665109238 9 0.09998102630692841 0.6169136958885394 >10 0.3867947457562927 4.407269849614089 >50 0.017467278680380748 0.8156092680894422 >100 0.012131364245440911 1.566074744929087 >500 0.0016171887214724001 0.730220580802009 >1k 6.289067250170445E-4 0.8598801609444154 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 3531 0.21753809226917306 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 2896 0.17841696834084542 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 2141 0.13190287611110152 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 1805 0.11120256486713603 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.160806917846872E-5 0.0 18-19 0.0 0.0 0.0 1.2321613835693745E-4 0.0 20-21 3.080403458923436E-5 0.0 0.0 2.464322767138749E-4 0.0 22-23 6.160806917846872E-5 0.0 0.0 2.464322767138749E-4 0.0 24-25 6.160806917846872E-5 0.0 0.0 2.464322767138749E-4 0.0 26-27 6.160806917846872E-5 0.0 0.0 3.080403458923436E-4 0.0 28-29 6.160806917846872E-5 0.0 0.0 0.001108945245212437 0.0 30-31 6.160806917846872E-5 0.0 0.0 0.004066132565778936 0.0 32-33 6.160806917846872E-5 0.0 0.0 0.011212668590481308 0.0 34-35 6.160806917846872E-5 0.0 0.0 0.021039155624447067 0.0 36-37 6.160806917846872E-5 0.0 0.0 0.036625997126599655 0.0 38-39 6.160806917846872E-5 0.0 0.0 0.059913847276060826 0.0 40-41 6.160806917846872E-5 0.0 0.0 0.0927817521827739 0.0 42-43 6.160806917846872E-5 0.0 0.0 0.12528000867441613 0.0 44-45 6.160806917846872E-5 0.0 0.0 0.16005776372566172 0.0 46-47 6.160806917846872E-5 0.0 0.0 0.2004726571067372 0.0 48-49 6.160806917846872E-5 0.0 0.0 0.2490506196539598 0.0 50-51 6.160806917846872E-5 0.0 0.0 0.3021259712512106 0.0 52-53 6.160806917846872E-5 0.0 0.0 0.3567415245779231 0.0 54-55 6.160806917846872E-5 0.0 0.0 0.4146839136402729 0.0 56-57 6.160806917846872E-5 0.0 0.0 0.4847938963653703 0.0 58-59 6.160806917846872E-5 0.0 0.0 0.5634057926370964 0.0 60-61 6.160806917846872E-5 0.0 0.0 0.6488561845876326 0.0 62-63 6.160806917846872E-5 0.0 0.0 0.7338753200539194 0.0 64-65 6.160806917846872E-5 0.0 0.0 0.8213279742527557 0.0 66-67 6.160806917846872E-5 0.0 0.0 0.9241210376770308 0.0 68-69 6.160806917846872E-5 0.0 0.0 1.0511568763230335 0.0 70-71 6.160806917846872E-5 0.0 0.0 1.1957818187194884 0.0 72-73 6.160806917846872E-5 0.0 0.0 1.3323977121227428 0.0 74-75 6.160806917846872E-5 0.0 0.0 1.4680586804537312 0.0 76-77 6.160806917846872E-5 0.0 0.0 1.6213703606043506 0.0 78-79 6.160806917846872E-5 0.0 0.0 1.6625553548501568 3.080403458923436E-5 80-81 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 82-83 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 84-85 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 86-87 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 88-89 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 90-91 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 92-93 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 94-95 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 96-97 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 98-99 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 100-101 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 102-103 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 104-105 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 106-107 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 108-109 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 110-111 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 112-113 6.160806917846872E-5 0.0 0.0 1.66323304361112 6.160806917846872E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAT 10 9.499266E-4 2933.2034 110-111 CGTCGCT 20 8.597217E-7 2933.2034 110-111 CCGCTCC 325 0.0 2898.987 108-109 CGTGTCG 25 1.6789309E-6 2346.563 110-111 CCGCGCC 230 0.0 2324.981 108-109 CCTCGCG 55 5.456968E-12 1851.9327 108-109 CGACGCG 15 0.0028362898 1697.605 108-109 CAGCGCG 65 1.4551915E-11 1567.0199 108-109 CGCGTCG 20 0.0037988466 1466.6017 110-111 CATCGCG 35 7.044562E-6 1455.09 108-109 CCGGTCG 55 1.46701495E-8 1388.9496 108-109 CGTCGCC 75 2.910383E-11 1358.084 108-109 CTCCGCC 195 0.0 1353.7863 110-111 CCGCACG 20 0.0050416356 1273.2037 108-109 CGTCGGC 25 0.005935026 1173.2815 110-111 TTACGCG 25 0.005935026 1173.2815 110-111 CGTCGCG 45 1.5216665E-6 1131.7366 108-109 CCGCGCA 45 1.4967878E-5 1131.7366 108-109 CGCCGCC 105 7.8216544E-11 1117.4109 110-111 CAGCTCG 115 0.0 1107.1338 108-109 >>END_MODULE