FastQCFastQC Report
Fri 27 May 2016
SRR939906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623164
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA33100.20392270898073145No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC30560.1882742594094004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24220.14921474355025124No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA20200.12444829974050681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18920.11656246688566281No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA18660.11496065708702263No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTA250.002057822771.3928159
GTATCAA63650.041.295571
CGTCACG659.1858965E-1041.1881660-61
TCAACGC70850.036.3317684
ATCAACG71150.036.178583
GGTATCA41800.035.9325071
CAACGCA71950.035.859135
TATCAAC74000.034.6241722
AACGCAG74650.034.5886156
GTACATG66200.034.3928871
TACATGG65550.033.633472
ACATGGG63600.032.790923
ACGCAGA83450.030.9411627
CGCAGAG83400.030.8883748
TAGGACC24600.029.3084564
CATGGGG34650.028.5461464
TATACCG1055.8571383E-428.3741845
GCAGAGT92750.027.5179829
GTAGGAC72650.027.2298413
CTACAGT77750.026.9764564
Produced by FastQC (version 0.11.5)