FastQCFastQC Report
Fri 27 May 2016
SRR939905_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939905_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334325
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA35700.26755100893710304No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC26950.20197478125644053No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA20900.1566335038315253No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA17080.12800479643265322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC3100.02182.9392108-109
CCGCGCT404.3655746E-91879.7533108-109
CGTCGCG504.8603397E-91828.624110-111
CCGCTCG651.6370905E-111542.3617108-109
TTGTTCG1550.01455.2928108-109
CGTCGCC1400.01432.193108-109
CCGCGCC2850.01319.1251108-109
CAGCGCG200.00520220261253.169108-109
CTCCGCC1950.01250.3412110-111
CTCGTCG250.00549449451219.0826110-111
CGTGTCG501.1946673E-51219.0826110-111
CCGTTAG451.5679929E-51113.9279108-109
CCTCTCG1850.01083.8218108-109
CGGCGGT300.0079112111015.9022110-111
AAGCGCG502.150562E-51002.53516108-109
CTGCGCG652.6237234E-5937.756110-111
CGCCGTC355.5567907E-5870.7734110-111
CCGCGCG901.117005E-7835.4459108-109
GTTCGTT754.0295425E-5812.7218110-111
CGAGTCG757.252756E-5668.35675108-109
Produced by FastQC (version 0.11.5)