##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939905_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1334325 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.954396417664363 34.0 31.0 34.0 30.0 34.0 2 31.96721938058569 34.0 31.0 34.0 30.0 34.0 3 32.15326850654826 34.0 31.0 34.0 30.0 34.0 4 35.38978584677646 37.0 35.0 37.0 33.0 37.0 5 35.47613812227156 37.0 35.0 37.0 33.0 37.0 6 35.493495962377985 37.0 37.0 37.0 35.0 37.0 7 35.43936522211605 37.0 37.0 37.0 35.0 37.0 8 35.45920671500571 37.0 37.0 37.0 35.0 37.0 9 37.093758267288706 39.0 39.0 39.0 35.0 39.0 10-11 37.07653645101456 39.0 39.0 39.0 35.0 39.0 12-13 37.01648361531112 39.0 38.5 39.0 35.0 39.0 14-15 38.55576115264272 41.0 39.5 41.0 35.0 41.0 16-17 38.56846870140333 41.0 39.0 41.0 35.0 41.0 18-19 38.58555149607479 41.0 39.5 41.0 34.0 41.0 20-21 38.65710752627733 41.0 39.5 41.0 34.5 41.0 22-23 38.65616472748393 41.0 39.0 41.0 34.0 41.0 24-25 38.695662975661854 41.0 39.0 41.0 34.0 41.0 26-27 38.68140520487887 41.0 39.0 41.0 34.0 41.0 28-29 38.62750079628276 41.0 39.0 41.0 34.0 41.0 30-31 38.54841698986379 41.0 39.0 41.0 34.0 41.0 32-33 38.469122964794934 41.0 39.0 41.0 34.0 41.0 34-35 38.434386674910535 40.5 39.0 41.0 34.0 41.0 36-37 38.58671050905889 41.0 39.0 41.0 34.0 41.0 38-39 38.535932025555994 41.0 39.0 41.0 34.0 41.0 40-41 38.44673336705825 41.0 39.0 41.0 33.0 41.0 42-43 38.40029640454912 41.0 39.0 41.0 33.0 41.0 44-45 38.32398366215128 41.0 39.0 41.0 33.0 41.0 46-47 38.23061135780263 41.0 39.0 41.0 33.0 41.0 48-49 38.15667397373204 41.0 38.5 41.0 33.0 41.0 50-51 38.05402469413374 40.0 38.0 41.0 33.0 41.0 52-53 37.975027073614 40.0 38.0 41.0 33.0 41.0 54-55 37.85607741742079 40.0 38.0 41.0 33.0 41.0 56-57 37.68364678770165 40.0 38.0 41.0 32.0 41.0 58-59 37.541105053116745 40.0 38.0 41.0 32.0 41.0 60-61 37.35942742585202 40.0 37.0 41.0 32.0 41.0 62-63 37.121434058419055 40.0 37.0 41.0 31.0 41.0 64-65 36.85382234463118 40.0 36.0 41.0 31.0 41.0 66-67 36.5702351376164 39.0 36.0 41.0 31.0 41.0 68-69 36.179889082494896 39.0 35.0 41.0 30.5 41.0 70-71 35.775112510070635 38.5 35.0 40.0 30.0 41.0 72-73 35.39381147771345 37.5 35.0 40.0 30.0 41.0 74-75 34.96614430517303 37.0 35.0 39.0 29.0 41.0 76-77 34.48249939107789 36.5 34.5 39.0 29.0 41.0 78-79 34.01450246379255 36.0 34.0 39.0 29.0 40.0 80-81 33.562717478875086 35.5 34.0 37.0 28.0 39.0 82-83 33.32376782268188 35.0 34.0 37.0 29.0 39.0 84-85 32.859101793041425 35.0 34.0 37.0 28.0 39.0 86-87 32.193453993592264 35.0 34.0 36.0 24.0 37.0 88-89 16.489160062203737 18.0 16.5 18.5 2.0 19.5 90-91 2.8400955539317634 2.0 2.0 2.0 2.0 2.0 92-93 2.3217559440166378 2.0 2.0 2.0 2.0 2.0 94-95 2.107097970884155 2.0 2.0 2.0 2.0 2.0 96-97 2.000023232720664 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.011815695930515346 1101 2 0.03147855690098211 1101 3 -0.0168362735000116 1101 4 0.11183190903673079 1101 5 0.10539983653922036 1101 6 0.049643619215700596 1101 7 -0.027376004402462684 1101 8 0.11724173208190791 1101 9 0.04494818948911927 1101 10-11 0.04899745471514194 1101 12-13 0.15682845837413595 1101 14-15 0.1381937083384983 1101 16-17 0.08314618910922178 1101 18-19 0.020318130832016834 1101 20-21 0.06471123524864453 1101 22-23 0.10865832878928217 1101 24-25 0.11529267353909489 1101 26-27 0.14496978836965724 1101 28-29 0.1333669518523024 1101 30-31 0.11690426888446126 1101 32-33 0.12465551041871947 1101 34-35 0.22416713243948294 1101 36-37 0.24923140871997873 1101 38-39 0.2456549432676738 1101 40-41 0.23271709707139365 1101 42-43 0.12682174935029877 1101 44-45 0.1457302085954737 1101 46-47 0.21544353020796336 1101 48-49 0.3003492852198235 1101 50-51 0.34434280098308534 1101 52-53 0.23412027866118024 1101 54-55 0.16151739206782167 1101 56-57 0.1466155118942254 1101 58-59 0.23738988901084213 1101 60-61 0.1410727616221905 1101 62-63 0.08192121248774953 1101 64-65 0.04133799005093408 1101 66-67 -0.0032755095899972275 1101 68-69 0.12242363564255498 1101 70-71 -0.010410235581517213 1101 72-73 -0.0096350925119566 1101 74-75 -0.008793624103198283 1101 76-77 0.002711790444550388 1101 78-79 0.10998404282999275 1101 80-81 0.050337483227451685 1101 82-83 -0.019890138008655356 1101 84-85 0.016821409934486553 1101 86-87 -0.054349116052442525 1101 88-89 0.40334599750659095 1101 90-91 -0.1745472294590713 1101 92-93 -0.21231084283910473 1101 94-95 -0.03359125946841335 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.08572632679462444 1102 2 -0.028964911969335816 1102 3 0.001248757958336455 1102 4 0.029260775596974042 1102 5 0.06823202735525769 1102 6 0.002424396753497149 1102 7 0.170408998884227 1102 8 0.08933770353463188 1102 9 0.0209658906817225 1102 10-11 0.05829821065466234 1102 12-13 0.0756923188305123 1102 14-15 0.045787731251444086 1102 16-17 -0.0016270290333295634 1102 18-19 -0.13250959947129815 1102 20-21 0.026717644301136545 1102 22-23 0.0449275794231383 1102 24-25 0.041578592404718506 1102 26-27 0.10594910567645144 1102 28-29 0.03244433000987357 1102 30-31 0.04004376555081279 1102 32-33 0.017522798426981012 1102 34-35 0.027861141266605216 1102 36-37 0.05878805723157399 1102 38-39 0.012886021303167183 1102 40-41 0.03436807368890982 1102 42-43 0.06956872089692467 1102 44-45 -0.00787224233888395 1102 46-47 0.06471869689028154 1102 48-49 -0.0438508744195758 1102 50-51 -0.023168232916809472 1102 52-53 0.032239233492617814 1102 54-55 0.04702542099072815 1102 56-57 0.0038738225337198173 1102 58-59 -0.09727181631359372 1102 60-61 -0.07488707028691266 1102 62-63 0.04553340612095269 1102 64-65 0.03611860325405303 1102 66-67 -0.004945854223294077 1102 68-69 -0.0851909291053019 1102 70-71 -0.020159390431743418 1102 72-73 0.0684696591079188 1102 74-75 0.08124769433045032 1102 76-77 0.031537267015124826 1102 78-79 0.13949444286755153 1102 80-81 0.1187987237195216 1102 82-83 0.15298466244209408 1102 84-85 0.10770494359961447 1102 86-87 0.026211852113583234 1102 88-89 0.780624380453343 1102 90-91 -0.013535632125988517 1102 92-93 0.00285490042317349 1102 94-95 0.1284092804883903 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.039703285593525095 1103 2 -0.09365128040024118 1103 3 0.01671115733002182 1103 4 -0.06399307487723149 1103 5 -0.04238948233200546 1103 6 -0.14430458374413035 1103 7 -0.12429782626109187 1103 8 0.005223164198831398 1103 9 -0.04603099058257243 1103 10-11 -0.07654430004173207 1103 12-13 -0.17675746404520964 1103 14-15 -0.10507026353391069 1103 16-17 -0.10739292294152136 1103 18-19 -0.09331478579634478 1103 20-21 -0.03319815440114127 1103 22-23 -0.03781480752839883 1103 24-25 -0.07854809767149362 1103 26-27 -0.017726116584839247 1103 28-29 -0.06647678378516275 1103 30-31 -0.0030734404638224078 1103 32-33 -0.004013829594157414 1103 34-35 -0.028874277616587563 1103 36-37 0.025638362484109223 1103 38-39 0.011838071312531895 1103 40-41 0.011531211420134468 1103 42-43 0.03530943204172843 1103 44-45 -0.011886530075358337 1103 46-47 -0.05029838081325977 1103 48-49 -0.10168789650643362 1103 50-51 -0.057647271633882724 1103 52-53 -0.036402632451981276 1103 54-55 -0.07562297009781815 1103 56-57 -0.03848824304649412 1103 58-59 0.117267405326686 1103 60-61 0.001899239239612882 1103 62-63 0.05556941085156808 1103 64-65 0.1128387024325832 1103 66-67 0.14837790524592265 1103 68-69 0.23385276515814013 1103 70-71 0.2172640681228586 1103 72-73 0.133678860976417 1103 74-75 0.1964274440096787 1103 76-77 0.2520074518104849 1103 78-79 0.1602822090217373 1103 80-81 0.15353917915538773 1103 82-83 0.1669727400132217 1103 84-85 0.12166847767967681 1103 86-87 0.07348489902246769 1103 88-89 0.5884859460312697 1103 90-91 -0.629266183407045 1103 92-93 -0.3383977993608438 1103 94-95 -0.11484125946841317 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.001040065227346787 1104 2 0.04460031392370212 1104 3 0.1235667502967317 1104 4 0.09623430660337817 1104 5 0.1911844133865941 1104 6 0.24413950398935924 1104 7 0.16668151930481656 1104 8 0.1960080587951012 1104 9 0.19810394185984848 1104 10-11 0.21255198146410947 1104 12-13 0.19243075753318806 1104 14-15 0.16467988654569155 1104 16-17 0.21586223849194397 1104 18-19 0.16358615731260784 1104 20-21 0.04414069490153594 1104 22-23 -0.0010404098043892418 1104 24-25 0.06591331229379449 1104 26-27 0.08728393222366293 1104 28-29 0.12631515367731794 1104 30-31 0.12256494163450782 1104 32-33 0.15197815310970952 1104 34-35 0.08295895566367761 1104 36-37 0.08432601419502106 1104 38-39 0.027927040243874046 1104 40-41 0.10181823323170391 1104 42-43 0.07729365845747793 1104 44-45 0.05189186542138202 1104 46-47 0.11252820546293663 1104 48-49 0.02627073807364866 1104 50-51 0.02148763905788087 1104 52-53 -7.864577854164168E-4 1104 54-55 -0.04416117297064659 1104 56-57 -0.047794266241403704 1104 58-59 0.04575229731286612 1104 60-61 0.11399837443306637 1104 62-63 0.04014383728645754 1104 64-65 -0.15436226938724928 1104 66-67 -0.1111168049916813 1104 68-69 -0.04982542501229403 1104 70-71 -0.21024585018163577 1104 72-73 -0.19235114189467595 1104 74-75 -0.3384970594225791 1104 76-77 -0.3687723748801943 1104 78-79 -0.26510430034409893 1104 80-81 -0.19785427737372174 1104 82-83 -0.1628484042459064 1104 84-85 -0.2693198944133428 1104 86-87 -0.1704890784786386 1104 88-89 0.38203404545792097 1104 90-91 -0.6877450287075955 1104 92-93 -0.3383977993608438 1104 94-95 -0.11484125946841317 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.1569978671551553 1105 2 -0.2266536321117627 1105 3 -0.121950742567833 1105 4 0.020294404040505754 1105 5 -0.09286813876833122 1105 6 -0.0808083726126938 1105 7 -0.07244139943733074 1105 8 -0.08177234617007656 1105 9 0.043969484510661516 1105 10-11 -0.07768155447791258 1105 12-13 -0.09896688514409391 1105 14-15 -0.13274741392468314 1105 16-17 -2.297046599295527E-4 1105 18-19 -0.023449355955740714 1105 20-21 -0.08173783656376798 1105 22-23 -0.22294973400772022 1105 24-25 -0.15007932317449502 1105 26-27 -0.04861311565840509 1105 28-29 -0.08859534330247243 1105 30-31 -0.15538833226133875 1105 32-33 -0.17664489594092458 1105 34-35 -0.11895195536084202 1105 36-37 -0.22619965447671575 1105 38-39 -0.12940417872903964 1105 40-41 -0.09701571591435254 1105 42-43 -0.10597735799082386 1105 44-45 -0.14988033010861557 1105 46-47 -0.08157733180739513 1105 48-49 -0.1535205686683554 1105 50-51 -0.2782479082588196 1105 52-53 -0.2627845599052421 1105 54-55 -0.21406217004080474 1105 56-57 -0.19298262506896435 1105 58-59 -0.2947275111756795 1105 60-61 -0.340072872787367 1105 62-63 -0.23728399913013476 1105 64-65 -0.2252020130578245 1105 66-67 -0.18373305277958707 1105 68-69 -0.12984298513284642 1105 70-71 -0.28724634829636386 1105 72-73 -0.2396018953720187 1105 74-75 -0.2846163358321192 1105 76-77 -0.16108611780255444 1105 78-79 -0.23309202192108813 1105 80-81 -0.29230613048662946 1105 82-83 -0.2161632316712172 1105 84-85 -0.22175860738576603 1105 86-87 -0.110679055653641 1105 88-89 0.6760693842810284 1105 90-91 -0.8250907077199412 1105 92-93 -0.3383977993608438 1105 94-95 -0.11484125946841317 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.08715727998754375 1106 2 -0.039319361808836106 1106 3 0.01601623821404985 1106 4 0.018854901299995674 1106 5 0.01252634847121925 1106 6 0.09660477209790486 1106 7 0.04393406424742352 1106 8 0.09455457941763967 1106 9 0.06535927154207855 1106 10-11 -0.052991893655793376 1106 12-13 0.038389017380339396 1106 14-15 -0.007965848161447298 1106 16-17 -0.12957310913639475 1106 18-19 -0.10168388507732118 1106 20-21 -0.09896630098324266 1106 22-23 -0.05381238604127958 1106 24-25 -0.15560808378900504 1106 26-27 -0.24482875690657835 1106 28-29 -0.12255818708199229 1106 30-31 -0.08953165788899753 1106 32-33 -0.05767599769940546 1106 34-35 -0.1262755503544568 1106 36-37 -0.0341298476818892 1106 38-39 -0.07283489545957877 1106 40-41 -0.02432428355408689 1106 42-43 -0.28767509057560403 1106 44-45 -0.05084955603349073 1106 46-47 -0.2125878253548521 1106 48-49 -0.09113349782474245 1106 50-51 -0.23576235320673788 1106 52-53 -0.127929546989904 1106 54-55 -0.12520212411394027 1106 56-57 -0.32447593956050014 1106 58-59 -0.15561299864306477 1106 60-61 -0.01930440411825174 1106 62-63 -0.006486627765056596 1106 64-65 -0.07208210911001345 1106 66-67 -0.15323328446196172 1106 68-69 -0.15513847615868315 1106 70-71 -0.09510498876883844 1106 72-73 -0.13554361381521574 1106 74-75 -0.22444688769696342 1106 76-77 -0.24918289191352017 1106 78-79 -0.15061514535786813 1106 80-81 -0.12290881185808189 1106 82-83 -0.09637550904494674 1106 84-85 -0.11477728789705566 1106 86-87 0.004394890703572685 1106 88-89 0.37114184582303267 1106 90-91 -0.21707065759462774 1106 92-93 -0.32361083194229767 1106 94-95 -0.11484125946841317 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.04241709123673232 1107 2 -0.05265091026454627 1107 3 5.3482660483439304E-5 1107 4 -0.0122101500524181 1107 5 0.045231256140986886 1107 6 0.06506152228197237 1107 7 0.06495025457486747 1107 8 0.026595363871358302 1107 9 0.04372952953126941 1107 10-11 0.025833508127597327 1107 12-13 0.03825477970901403 1107 14-15 0.06035294585623774 1107 16-17 0.054338078327816675 1107 18-19 -0.006995011558210251 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0.04902059359534405 2216 82-83 0.015038791757234549 2216 84-85 0.06847274772712808 2216 86-87 0.1641500478271567 2216 88-89 -0.8848647048345484 2216 90-91 -0.8250907077199412 2216 92-93 -0.3383977993608438 2216 94-95 -0.11484125946841317 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11710.0 3 2099.0 4 1541.0 5 1556.0 6 1649.0 7 1538.0 8 1790.0 9 2090.0 10 2500.0 11 2989.0 12 3767.0 13 4335.0 14 4759.0 15 5373.0 16 6163.0 17 7300.0 18 9424.0 19 12072.0 20 11882.0 21 17408.0 22 20781.0 23 24487.0 24 37554.0 25 64119.0 26 117246.0 27 352160.0 28 583058.0 29 22166.0 30 809.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.84387236992487 20.691660577445525 14.373109999437919 24.09135705319169 2 12.570185773085235 20.392320859526528 45.70176779713351 21.33572557025472 3 18.317308269053132 26.498038604109937 29.513384877457742 25.671268249379192 4 11.079302404573257 17.506918893546892 40.64297909448161 30.770799607398246 5 11.651760139887406 40.16424173167652 36.094265036246554 12.089733092189519 6 29.344500028104097 38.31203042736964 19.89447848162929 12.44899106289697 7 27.56907050381279 32.55451258126768 22.84158656998857 17.034830344930956 8 23.477188840799656 40.22498266913983 21.061135780263427 15.23669270979709 9 26.158694470987204 17.305529012796733 22.14318100912446 34.3925955070916 10-11 23.47842281561056 27.243352298127615 30.73034880457343 18.54787608168839 12-13 25.71276217428109 25.171991124924446 29.429659105514993 19.685587595279465 14-15 19.86874257348943 30.353073783775987 26.17159469079673 23.606588951937855 16-17 19.54590370154697 30.958716440315502 30.18116711839472 19.314212739742814 18-19 20.780204860269226 28.777572623538806 30.973601558655893 19.46862095753607 20-21 21.840208530535737 28.937321674689663 28.837230914741856 20.385238880032734 22-23 20.461218965279485 31.417764360959055 28.88582280606035 19.23519386770111 24-25 20.93151968223634 29.325838907312686 30.64380866730369 19.098832743147284 26-27 19.12506323421955 31.02763569595114 29.88720888838926 19.960092181440054 28-29 19.57192904355542 30.542156010796578 28.358895205504865 21.52701974014314 30-31 20.688587862777062 29.166844659284656 29.628913495587657 20.515653982350628 32-33 19.276341823152745 30.81599095858215 30.245468798714537 19.662198419550567 34-35 19.644119152559014 30.823009121479583 30.327027264013285 19.205844461948118 36-37 19.145715951621874 29.890311334145732 30.060847381666495 20.903125332565903 38-39 20.63069342176756 30.84184887489929 28.7423228973451 19.785134805988047 40-41 22.137260412568153 28.668877522342758 30.030127592603 19.163734472486087 42-43 19.504843272815844 30.92889663312911 30.296104772075765 19.270155321979278 44-45 19.075216420611802 31.04173427273606 29.521862397635356 20.36118690901678 46-47 20.76997732936129 29.084705750098365 29.496224682892098 20.649092237648247 48-49 20.1575857092912 29.73552475921283 29.11090018410106 20.99598934739491 50-51 19.38155154336694 31.31235840197613 29.128554330548152 20.17753572410878 52-53 19.238978509733386 30.474209806456447 30.052610870664942 20.234200813145222 54-55 19.465803894764612 29.537346811521214 30.612926289661125 20.383923004053052 56-57 20.064901729338803 29.803571093998837 28.479306016150492 21.65222116051187 58-59 20.557360463155526 30.113353193562286 28.002585576977125 21.32670076630506 60-61 19.78161242575834 28.898694096265903 30.451089502182754 20.868603975793004 62-63 20.31206789949975 29.08421861240702 30.127967324302553 20.47574616379068 64-65 20.549903509264983 29.806643808667303 28.72077642253574 20.922676259531972 66-67 20.634478106907988 30.92215164971053 27.970097240177616 20.473273003203868 68-69 21.401155119778196 28.841908671867067 29.977467932179714 19.779468276175027 70-71 21.883574091769248 29.008375021078077 29.167032019935174 19.941018867217508 72-73 20.91649335806494 30.22947932475222 28.561107676165854 20.292919641016994 74-75 19.855507466321924 30.70249002304536 29.91002941562213 19.531973095010585 76-77 20.56305622693122 28.980758061191985 30.410619601671257 20.045566110205534 78-79 20.11108267385334 29.07766231751493 29.590732068695587 21.22052293993614 80-81 20.55076536825736 30.14734041556592 29.281921570831692 20.019972645345025 82-83 20.032878317997113 30.159018042229942 30.20608342564044 19.602020214132505 84-85 19.93185313312209 29.480545954355915 30.327266840737334 20.260334071784662 86-87 19.09848367036834 31.216979594283927 28.5720870189218 21.112449716425935 88-89 29.473592361524314 18.464275403956062 35.89446784178345 16.16766439273617 90-91 31.444881431592258 16.23510303811376 18.185130658253744 34.13488487204024 92-93 22.974582465728975 18.278928542921197 37.00026460230776 21.74622438904207 94-95 17.024018666865633 7.561505016041838 29.64246711932007 45.772009197772455 96-97 29.147699342336814 7.676857876687425 16.51037106631025 46.665071714665515 98-99 57.538853530691455 14.228570503166043 18.615146264177017 9.617429701965484 100-101 31.230805446766457 7.4626169930598545 13.197461987695286 48.1091155724784 102-103 24.01291422884999 2.2301522925476007 38.270562053733556 35.48637142486885 104-105 41.64509733425429 4.710443833458398 42.44650681242811 11.197952019859201 106-107 6.999473032757754 2.2663676433943225 8.092623684113775 82.64153563973414 108-109 19.363005105186744 12.596338638622587 23.844257929848062 44.19639832634261 110-111 41.24206504907031 3.006702196879032 26.998020951591418 28.753211802459234 112-113 28.21030134577278 2.8944714812418693 40.34251767387755 28.5527094991078 114-115 30.664890671195643 4.2953340357793035 25.54309392422104 39.49668136880401 116-117 21.487816710746646 4.663497975599887 22.091224320523683 51.757460993129776 118-119 22.361358853056565 4.600957925617691 17.515704685063902 55.52197853626184 120-121 27.15044451036872 4.454864732958841 31.572389637980038 36.8223011186924 122-123 35.33860999539898 1.7911324719897532 35.497780320081574 27.372477212529688 124-125 39.79057159967411 2.300699368836676 21.217610813346994 36.691118218142215 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 34.0 1 85.0 2 181.0 3 295.0 4 380.0 5 372.0 6 341.5 7 305.5 8 254.0 9 229.5 10 198.5 11 164.5 12 150.0 13 158.5 14 200.0 15 246.0 16 307.0 17 406.0 18 568.0 19 862.0 20 1314.0 21 1949.5 22 2789.0 23 4030.0 24 5701.0 25 7821.5 26 10441.0 27 13491.0 28 17458.5 29 22311.0 30 28307.5 31 35753.0 32 44328.5 33 53621.5 34 62415.0 35 68698.5 36 71343.0 37 71101.0 38 68738.0 39 65980.5 40 63032.5 41 59175.5 42 55042.0 43 51198.5 44 47879.5 45 44812.5 46 42061.5 47 39618.0 48 36831.0 49 33791.5 50 30837.0 51 27895.0 52 24798.0 53 21571.0 54 18499.0 55 15391.5 56 12575.0 57 10245.5 58 8315.0 59 6646.5 60 5207.0 61 4024.5 62 3072.5 63 2270.5 64 1653.0 65 1236.0 66 909.0 67 671.0 68 493.0 69 351.0 70 253.0 71 174.0 72 113.5 73 88.5 74 75.0 75 58.5 76 35.0 77 21.0 78 11.5 79 6.0 80 5.0 81 4.5 82 4.0 83 4.5 84 3.5 85 1.5 86 2.0 87 5.0 88 4.0 89 3.5 90 4.0 91 3.0 92 3.0 93 1.5 94 1.5 95 3.0 96 6.0 97 8.0 98 4.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 6.744983418582429E-4 3 0.0435426151799599 4 0.049313323215858205 5 0.05103704120060704 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.869147321679501E-4 12-13 0.001986022895471493 14-15 3.7472130103235724E-5 16-17 3.3724917092912147E-4 18-19 0.007382009630337437 20-21 0.0026979933674329717 22-23 9.742753826841287E-4 24-25 0.0 26-27 0.0 28-29 0.0010117475127873643 30-31 0.0 32-33 0.0015738294643359001 34-35 1.498885204129429E-4 36-37 2.248327806194143E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.498885204129429E-4 46-47 0.0 48-49 2.6230491072265006E-4 50-51 1.498885204129429E-4 52-53 0.0 54-55 0.0015738294643359001 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.3724917092912147E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.6230491072265006E-4 80-81 0.0 82-83 8.243868622711859E-4 84-85 7.494426020647145E-5 86-87 0.0013115245536132502 88-89 19.26382253199183 90-91 26.263578963146163 92-93 40.37933037303505 94-95 44.75596275270268 96-97 48.46937590167313 98-99 93.05840031476589 100-101 51.76778520975025 102-103 53.15631499072565 104-105 74.88171922132913 106-107 81.65386993423641 108-109 12.609821445300057 110-111 39.06904239971521 112-113 52.099413561163885 114-115 86.8059505742604 116-117 66.47795701946677 118-119 71.9758679482135 120-121 80.42137410301089 122-123 75.89282970790474 124-125 50.00457159987259 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1334325.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.41278774463574 #Duplication Level Percentage of deduplicated Percentage of total 1 78.47213326824544 52.11553130612754 2 13.327113324400672 17.701814969242573 3 4.10119426335769 8.171152323356761 4 1.7129083020836489 4.55036061969223 5 0.8311983766201877 2.7601100680081156 6 0.45305219337399794 1.8053075493473396 7 0.2648633139299634 1.2313217734560036 8 0.17081355127337733 0.9075363299701008 9 0.117276268843057 0.7009779555141104 >10 0.5093048046787619 5.601289758984936 >50 0.024009822434489566 1.1184438990853336 >100 0.01365062866662199 1.6523532668312733 >500 0.0018050051578890093 0.7585866702138511 >1k 6.768769342083785E-4 0.9252135101699601 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 3570 0.26755100893710304 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 2695 0.20197478125644053 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 2090 0.1566335038315253 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 1708 0.12800479643265322 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.494426020647143E-5 0.0 14-15 0.0 0.0 0.0 1.1241639030970715E-4 0.0 16-17 0.0 0.0 0.0 1.873606505161786E-4 0.0 18-19 0.0 0.0 0.0 2.9977704082588574E-4 0.0 20-21 0.0 0.0 0.0 3.747213010323572E-4 0.0 22-23 0.0 0.0 0.0 5.620819515485358E-4 0.0 24-25 0.0 0.0 0.0 7.119704719614787E-4 0.0 26-27 0.0 0.0 0.0 0.001199108163303543 0.0 28-29 0.0 0.0 0.0 0.00236074419650385 0.0 30-31 0.0 0.0 0.0 0.006145429336930658 0.0 32-33 0.0 0.0 0.0 0.013639855357577802 0.0 34-35 0.0 0.0 0.0 0.021996140370599368 0.0 36-37 0.0 0.0 0.0 0.03754707436344219 0.0 38-39 0.0 0.0 0.0 0.06377756543570719 0.0 40-41 0.0 0.0 0.0 0.09532909898263167 0.0 42-43 0.0 0.0 0.0 0.1274801866112079 0.0 44-45 0.0 0.0 0.0 0.1657392314466116 0.0 46-47 0.0 0.0 0.0 0.21048095478987502 0.0 48-49 0.0 0.0 0.0 0.2581455042811909 0.0 50-51 0.0 0.0 0.0 0.31015682086448204 0.0 52-53 0.0 0.0 0.0 0.3644539373840706 0.0 54-55 0.0 0.0 0.0 0.42186124070222775 0.0 56-57 0.0 0.0 0.0 0.48901129784722613 0.0 58-59 0.0 0.0 0.0 0.5677402431941244 0.0 60-61 0.0 0.0 0.0 0.6568489685796188 0.0 62-63 0.0 0.0 0.0 0.7486182152024432 0.0 64-65 0.0 0.0 0.0 0.8437599535345587 0.0 66-67 0.0 0.0 0.0 0.9483821407827928 0.0 68-69 0.0 0.0 0.0 1.0787851535420532 0.0 70-71 0.0 0.0 0.0 1.2316714443632548 0.0 72-73 0.0 0.0 0.0 1.3704307421355366 0.0 74-75 0.0 0.0 0.0 1.5138740561707231 0.0 76-77 0.0 0.0 0.0 1.6649991568770726 0.0 78-79 0.0 0.0 0.0 1.706255972120735 0.0 80-81 0.0 0.0 0.0 1.7072677196335224 0.0 82-83 0.0 0.0 0.0 1.7073051917636257 0.0 84-85 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 86-87 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 88-89 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 90-91 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 92-93 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 94-95 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 96-97 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 98-99 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 100-101 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 102-103 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 104-105 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 106-107 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 108-109 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 110-111 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 112-113 0.0 0.0 7.494426020647143E-5 1.7073051917636257 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 310 0.0 2182.9392 108-109 CCGCGCT 40 4.3655746E-9 1879.7533 108-109 CGTCGCG 50 4.8603397E-9 1828.624 110-111 CCGCTCG 65 1.6370905E-11 1542.3617 108-109 TTGTTCG 155 0.0 1455.2928 108-109 CGTCGCC 140 0.0 1432.193 108-109 CCGCGCC 285 0.0 1319.1251 108-109 CAGCGCG 20 0.0052022026 1253.169 108-109 CTCCGCC 195 0.0 1250.3412 110-111 CTCGTCG 25 0.0054944945 1219.0826 110-111 CGTGTCG 50 1.1946673E-5 1219.0826 110-111 CCGTTAG 45 1.5679929E-5 1113.9279 108-109 CCTCTCG 185 0.0 1083.8218 108-109 CGGCGGT 30 0.007911211 1015.9022 110-111 AAGCGCG 50 2.150562E-5 1002.53516 108-109 CTGCGCG 65 2.6237234E-5 937.756 110-111 CGCCGTC 35 5.5567907E-5 870.7734 110-111 CCGCGCG 90 1.117005E-7 835.4459 108-109 GTTCGTT 75 4.0295425E-5 812.7218 110-111 CGAGTCG 75 7.252756E-5 668.35675 108-109 >>END_MODULE