FastQCFastQC Report
Fri 27 May 2016
SRR939905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939905_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334325
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42300.31701422067337415No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA34680.25990669439604297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31330.23480036722687503No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC26830.20107545013396286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20410.15296123508140821No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA19610.14696569426489048No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA16940.12695557678976263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52300.057.5319331
GGTATCA29300.055.9222151
ATCAACG65200.045.7783933
TCAACGC66400.044.9510734
CAACGCA67650.044.2085535
TATCAAC68250.043.90722
AACGCAG70100.042.690786
CGCAGAG80850.036.8673448
ACGCAGA81050.036.8497777
TAGGACC24500.035.9886864
TTCGCGT350.002790677133.99743326-27
GTACATG65250.032.59921
GCAGAGT92150.032.281889
TACATGG66100.031.365212
ATGGGCG1551.94932E-730.7488385
ACATGGG64250.030.2283823
TTACGCA800.005329204729.7879373
CTACAGT66850.029.3200634
TACAGTG69600.028.9319615
CGGGAGT2502.1827873E-1128.5964184
Produced by FastQC (version 0.11.5)