##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939905_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1334325 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.829077623517506 34.0 31.0 34.0 31.0 34.0 2 32.932294605886874 34.0 33.0 34.0 31.0 34.0 3 32.969911378412306 34.0 33.0 34.0 31.0 34.0 4 36.36465853521443 37.0 37.0 37.0 35.0 37.0 5 36.275643490154195 37.0 37.0 37.0 35.0 37.0 6 36.41599310512806 37.0 37.0 37.0 35.0 37.0 7 36.40785940456785 37.0 37.0 37.0 35.0 37.0 8 36.436749667434846 37.0 37.0 37.0 35.0 37.0 9 38.275378187473066 39.0 39.0 39.0 37.0 39.0 10-11 38.265921720720215 39.0 39.0 39.0 37.0 39.0 12-13 38.25556030202537 39.0 39.0 39.0 37.0 39.0 14-15 39.79955932774999 41.0 40.0 41.0 37.5 41.0 16-17 39.80320911322204 41.0 40.0 41.0 37.5 41.0 18-19 39.85892454986604 41.0 40.0 41.0 38.0 41.0 20-21 39.877853221666385 41.0 40.0 41.0 38.0 41.0 22-23 39.82881756693459 41.0 40.0 41.0 38.0 41.0 24-25 39.803957806381504 41.0 40.0 41.0 37.0 41.0 26-27 39.71769733760516 41.0 40.0 41.0 37.0 41.0 28-29 39.632545294437264 41.0 40.0 41.0 37.0 41.0 30-31 39.4950634215802 41.0 39.5 41.0 36.5 41.0 32-33 39.345038877334986 41.0 39.0 41.0 35.5 41.0 34-35 39.26643621306653 41.0 39.0 41.0 35.0 41.0 36-37 39.24517302756075 41.0 39.5 41.0 35.0 41.0 38-39 39.28505686395743 41.0 40.0 41.0 35.0 41.0 40-41 39.17667772094505 41.0 40.0 41.0 35.0 41.0 42-43 39.089040526108704 41.0 40.0 41.0 35.0 41.0 44-45 38.93818222696869 41.0 40.0 41.0 35.0 41.0 46-47 38.694517077923294 41.0 39.0 41.0 35.0 41.0 48-49 38.44314091394526 41.0 39.0 41.0 35.0 41.0 50-51 38.28975474490847 41.0 39.0 41.0 35.0 41.0 52-53 38.196836602776685 41.0 39.0 41.0 35.0 41.0 54-55 38.11252543420831 41.0 39.0 41.0 34.5 41.0 56-57 37.989397260787285 41.0 39.0 41.0 34.0 41.0 58-59 37.85642403462425 41.0 38.0 41.0 34.0 41.0 60-61 37.711105240477394 40.0 38.0 41.0 34.0 41.0 62-63 37.52388698405561 40.0 37.5 41.0 34.0 41.0 64-65 37.30830869540779 40.0 37.0 41.0 33.0 41.0 66-67 37.0565488168175 39.5 37.0 41.0 33.0 41.0 68-69 36.73510801341503 39.0 36.0 41.0 32.5 41.0 70-71 36.37426713881551 39.0 35.5 41.0 32.0 41.0 72-73 36.039128398253794 38.0 35.0 40.0 32.0 41.0 74-75 35.674111629475576 37.0 35.0 39.5 32.0 41.0 76-77 35.26966368763232 37.0 35.0 39.0 31.0 41.0 78-79 34.87908942723849 36.0 35.0 39.0 31.0 40.5 80-81 34.56615254904165 36.0 35.0 38.0 31.0 39.0 82-83 34.2845000281041 35.5 35.0 37.0 31.0 39.0 84-85 33.9705630187548 35.0 35.0 37.0 31.0 39.0 86-87 33.71700822513256 35.0 35.0 36.0 31.0 37.0 88-89 33.47343563224851 35.0 35.0 36.0 31.0 37.0 90-91 33.266759597549324 35.0 35.0 36.0 31.0 37.0 92-93 33.11945140801529 35.0 35.0 35.5 31.0 36.0 94-95 33.00693609128211 35.0 35.0 35.0 31.0 36.0 96-97 32.88017724317538 35.0 34.0 35.0 30.0 36.0 98-99 32.762938189721396 35.0 34.0 35.0 30.0 36.0 100-101 32.74739699848238 35.0 34.0 35.0 30.0 36.0 102-103 31.942670263991154 34.5 33.0 35.0 28.0 35.0 104-105 32.587752608997064 35.0 34.0 35.0 29.5 35.0 106-107 32.65327899874468 35.0 34.0 35.0 30.0 35.0 108-109 32.64578307383883 35.0 34.0 35.0 30.0 35.0 110-111 32.626978809510426 35.0 34.0 35.0 30.0 35.0 112-113 32.55284207370768 35.0 34.0 35.0 30.0 35.0 114-115 32.49885035504843 35.0 34.0 35.0 29.5 35.0 116-117 32.44235549809829 35.0 34.0 35.0 29.0 35.0 118-119 32.364687014033315 35.0 34.0 35.0 29.0 35.0 120-121 32.26732167950087 35.0 34.0 35.0 29.0 35.0 122-123 32.108760234575534 35.0 34.0 35.0 29.0 35.0 124-125 30.117124763457177 33.5 30.5 34.5 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.01900677717763699 1101 2 0.049460946115161164 1101 3 0.07185154762802881 1101 4 0.003082458586867176 1101 5 -0.03186134861932999 1101 6 0.01057364919311965 1101 7 0.027461257951387097 1101 8 0.07397065820668303 1101 9 0.03746317177316172 1101 10-11 0.12498161560337451 1101 12-13 0.06273428145480864 1101 14-15 0.1500281091676925 1101 16-17 0.16248621047400746 1101 18-19 0.11278039042093013 1101 20-21 0.1680327408932527 1101 22-23 0.18790791074943058 1101 24-25 0.1637765445856303 1101 26-27 0.1595105193776476 1101 28-29 0.1780751574197197 1101 30-31 0.1489640473912175 1101 32-33 0.16468944933313878 1101 34-35 0.15045988369543295 1101 36-37 0.059697994517094344 1101 38-39 -0.007180406660793892 1101 40-41 -0.004562055108785046 1101 42-43 -0.007932771407290318 1101 44-45 0.04701818977385841 1101 46-47 0.07499403727825182 1101 48-49 -0.028764559021901448 1101 50-51 0.017995766817648473 1101 52-53 -0.027581770118828786 1101 54-55 -0.025557009850274426 1101 56-57 0.07200003522019927 1101 58-59 0.0699546107020268 1101 60-61 0.07690367744211812 1101 62-63 -0.014802483152358548 1101 64-65 0.05218891693327521 1101 66-67 0.06914513405263278 1101 68-69 -0.061473953154298044 1101 70-71 0.007212066022660224 1101 72-73 -0.013452099840307596 1101 74-75 0.06619913726338211 1101 76-77 0.0725145797818314 1101 78-79 -9.703995124539233E-4 1101 80-81 -0.021788912550078976 1101 82-83 -0.008107249959095952 1101 84-85 -0.014640119439128796 1101 86-87 -0.05874446487547402 1101 88-89 -0.044279518094867854 1101 90-91 -0.04070961923451932 1101 92-93 -0.07486250917887105 1101 94-95 -0.10299920525184803 1101 96-97 -0.043605818617486136 1101 98-99 -0.0601218655467477 1101 100-101 -0.02786012587402098 1101 102-103 0.015583060676437555 1101 104-105 -0.07335296156948345 1101 106-107 -0.03397692451450496 1101 108-109 0.018381366195924898 1101 110-111 3.731160887809892E-5 1101 112-113 0.02932594413277201 1101 114-115 0.004437839698191226 1101 116-117 0.022734546770500685 1101 118-119 -0.005347824685486557 1101 120-121 -0.015656861612406203 1101 122-123 0.016099231580071205 1101 124-125 0.1307984687624142 1102 1 0.018939274094783798 1102 2 0.0776619509043428 1102 3 0.12503611894927502 1102 4 0.07302799337886512 1102 5 -0.06477758488564689 1102 6 -0.029475885974498794 1102 7 0.033752928530780935 1102 8 -0.015767814889379395 1102 9 -7.845510177162396E-4 1102 10-11 0.11866470791251515 1102 12-13 0.09495444297232325 1102 14-15 0.05480790069741204 1102 16-17 0.16995346092005548 1102 18-19 0.031094903520752837 1102 20-21 0.035389417576510596 1102 22-23 0.13943211490006036 1102 24-25 0.09266071211309423 1102 26-27 0.10010822808268927 1102 28-29 0.13202447190933952 1102 30-31 0.07212115134463915 1102 32-33 0.15216655985994976 1102 34-35 0.14264166132713285 1102 36-37 0.1600530748063278 1102 38-39 0.1490050332866133 1102 40-41 0.12641327121293244 1102 42-43 0.14226443020789503 1102 44-45 0.2537206970515413 1102 46-47 0.29147093651761935 1102 48-49 0.21652431786795034 1102 50-51 0.24216118141056597 1102 52-53 0.3008763010615638 1102 54-55 0.16789836154610782 1102 56-57 0.2002474058653334 1102 58-59 0.19902600238199852 1102 60-61 0.2144870186009129 1102 62-63 0.11997132189976156 1102 64-65 0.08395422353483184 1102 66-67 0.17288726946441102 1102 68-69 0.1996556130021503 1102 70-71 0.15817577153491413 1102 72-73 0.20679041119359454 1102 74-75 0.14928322966642327 1102 76-77 0.16201418068332174 1102 78-79 0.15872241201914505 1102 80-81 0.21647876516613707 1102 82-83 0.13795458683308937 1102 84-85 0.17097531393489618 1102 86-87 0.1685069202311098 1102 88-89 0.2044257593959813 1102 90-91 0.2689867137544937 1102 92-93 0.21441171844813312 1102 94-95 0.252784788550386 1102 96-97 0.303742523949893 1102 98-99 0.3701996435151358 1102 100-101 0.3645710883244888 1102 102-103 0.38189151686951206 1102 104-105 0.3290874084183031 1102 106-107 0.30161972775331947 1102 108-109 0.35509373355436225 1102 110-111 0.28245174019372143 1102 112-113 0.2504458849722795 1102 114-115 0.19959994412114668 1102 116-117 0.23752525481820186 1102 118-119 0.23713621137423502 1102 120-121 0.29559067336739986 1102 122-123 0.34956598902208924 1102 124-125 0.22881412750971108 1103 1 0.01210476800114435 1103 2 0.07693934486504617 1103 3 0.08972888010343638 1103 4 0.07531795817322262 1103 5 -0.08462584903297454 1103 6 0.04259750248846217 1103 7 0.005699147463289478 1103 8 0.02965362539116967 1103 9 0.05405017425500347 1103 10-11 0.05713702970393797 1103 12-13 0.06850112583389034 1103 14-15 0.05902709804534112 1103 16-17 0.10219401909612458 1103 18-19 0.11222369862939985 1103 20-21 0.1543751207075701 1103 22-23 0.03875300221004352 1103 24-25 0.11291594158902285 1103 26-27 0.1267110892829706 1103 28-29 0.15116953649867781 1103 30-31 -0.009522486297541377 1103 32-33 0.012557474886961018 1103 34-35 0.049467719893613094 1103 36-37 0.05954804966923177 1103 38-39 0.011815479909664361 1103 40-41 -0.03885346516485555 1103 42-43 -0.010108982456095816 1103 44-45 -0.2189138811885485 1103 46-47 -0.21087667694180112 1103 48-49 -0.17832851251026938 1103 50-51 -0.08823987064903349 1103 52-53 -0.19610599109502402 1103 54-55 -0.20030480376514959 1103 56-57 -0.1289013573710278 1103 58-59 -0.1610138836448698 1103 60-61 -0.07919982471801035 1103 62-63 -0.1794758216945027 1103 64-65 -0.241718496431929 1103 66-67 -0.1929768440703583 1103 68-69 -0.16965657563797976 1103 70-71 -0.20533159607863638 1103 72-73 -0.23199355585648362 1103 74-75 -0.25007418749073906 1103 76-77 -0.21143672548519987 1103 78-79 -0.3399830615218349 1103 80-81 -0.32199458402723735 1103 82-83 -0.22294432138110665 1103 84-85 -0.20528896124444174 1103 86-87 -0.22976202163620485 1103 88-89 -0.1929179499360174 1103 90-91 -0.21570100171452822 1103 92-93 -0.18804674026628732 1103 94-95 -0.20362368820065058 1103 96-97 -0.19137560444339385 1103 98-99 -0.15809215233780805 1103 100-101 -0.13019030328003822 1103 102-103 -0.2378978428992582 1103 104-105 -0.2613102646304313 1103 106-107 -0.19979352070900802 1103 108-109 -0.19071184090031323 1103 110-111 -0.08939014036280923 1103 112-113 -0.1623259769996892 1103 114-115 -0.2009492592998754 1103 116-117 -0.08353728428014762 1103 118-119 -0.10206282606429085 1103 120-121 -0.03951245495738931 1103 122-123 -0.015869469587315166 1103 124-125 0.032899420136619284 1104 1 -6.291604304564657E-4 1104 2 0.063338652323786 1104 3 0.0711559849155563 1104 4 0.05778365737019442 1104 5 -0.028600479054112782 1104 6 0.016323783385281843 1104 7 -0.002553055879353394 1104 8 0.013427627253022933 1104 9 -0.03004443244942223 1104 10-11 0.047963141815579036 1104 12-13 0.06881542298280863 1104 14-15 0.02124836323818613 1104 16-17 0.08010675976725423 1104 18-19 0.11015357882673271 1104 20-21 0.1512218176511695 1104 22-23 0.0794191381604179 1104 24-25 0.08612401282681503 1104 26-27 0.06403055874067576 1104 28-29 0.05831647965982967 1104 30-31 0.03980095918223725 1104 32-33 0.08255601759221776 1104 34-35 0.12440312041040613 1104 36-37 0.10493081792021286 1104 38-39 0.03545356184878301 1104 40-41 0.08661145535820935 1104 42-43 0.12877773136602144 1104 44-45 0.12171625740671033 1104 46-47 0.21196174169764959 1104 48-49 0.20840107883818604 1104 50-51 0.19983956713970485 1104 52-53 0.19171058987400613 1104 54-55 0.14921374520429964 1104 56-57 0.2300705755673107 1104 58-59 0.16229782952471794 1104 60-61 0.11795529669421967 1104 62-63 0.2523662858582014 1104 64-65 0.18380123577385632 1104 66-67 0.12914021367330974 1104 68-69 0.1399853866202605 1104 70-71 0.1467361311505897 1104 72-73 0.20673263803767128 1104 74-75 0.14141608126051608 1104 76-77 0.1401731501879837 1104 78-79 0.08065310200838383 1104 80-81 0.10708029482154302 1104 82-83 0.10303293612069808 1104 84-85 0.1119467028904495 1104 86-87 -0.0011983922327800656 1104 88-89 0.05348394585217875 1104 90-91 0.08086065272826204 1104 92-93 -0.014826053641215253 1104 94-95 0.006947788843689295 1104 96-97 0.03270031843744192 1104 98-99 0.01666848156364864 1104 100-101 0.12814944650028082 1104 102-103 0.1700729068027549 1104 104-105 0.11042767897443184 1104 106-107 0.09310640881882648 1104 108-109 0.08051681081929729 1104 110-111 0.0685077446022575 1104 112-113 0.08811664908893135 1104 114-115 0.05655472107231674 1104 116-117 -0.005883050295167891 1104 118-119 0.07517194629321722 1104 120-121 0.024070728296344157 1104 122-123 0.06884122478100352 1104 124-125 0.02048468275418358 1105 1 0.001617084793316792 1105 2 0.0368177209963676 1105 3 0.02949299631124802 1105 4 0.016430151441156227 1105 5 -0.05745645453929882 1105 6 0.029033590570747947 1105 7 -0.026975389914674963 1105 8 0.019872643373446408 1105 9 0.02126874089870512 1105 10-11 0.0692073672576825 1105 12-13 0.1259903768494013 1105 14-15 0.09099493423319416 1105 16-17 0.044885897377234585 1105 18-19 -0.015335866185189673 1105 20-21 0.04752962102826075 1105 22-23 0.04198066688923774 1105 24-25 0.01739547694783994 1105 26-27 0.0916462390561108 1105 28-29 0.11309675221475857 1105 30-31 -0.008251611888439925 1105 32-33 0.047533245131873514 1105 34-35 0.04935488524981224 1105 36-37 -0.008134920613692032 1105 38-39 0.013807902172530362 1105 40-41 0.06259081827577262 1105 42-43 0.07061232785105176 1105 44-45 0.052676137102544374 1105 46-47 0.07661114879400088 1105 48-49 0.10966113045716241 1105 50-51 0.07141818099360364 1105 52-53 0.05762052148306651 1105 54-55 0.03517515917757663 1105 56-57 0.128804061067342 1105 58-59 0.05372825950071558 1105 60-61 0.1847055604638328 1105 62-63 0.08836096002390548 1105 64-65 0.1356866630806266 1105 66-67 0.07886556750291618 1105 68-69 0.040382144460835434 1105 70-71 0.13110567973673426 1105 72-73 0.06872858586383046 1105 74-75 0.013163097826506487 1105 76-77 0.05165506252819796 1105 78-79 0.02927424897535502 1105 80-81 -0.11333363431585752 1105 82-83 0.02512264301066125 1105 84-85 -0.04070181726743982 1105 86-87 -0.07791331507754506 1105 88-89 -0.05151238659421864 1105 90-91 -0.0021584689924765144 1105 92-93 -0.02639329702805071 1105 94-95 -0.023895916625477298 1105 96-97 0.031986511621887814 1105 98-99 0.010049282918856761 1105 100-101 0.15737769443951777 1105 102-103 0.18508360470983476 1105 104-105 0.17263395942353554 1105 106-107 0.10310811123626706 1105 108-109 0.13924338156208904 1105 110-111 0.004621903250750847 1105 112-113 -0.007077384463400449 1105 114-115 0.06742497164294292 1105 116-117 0.0851920788573679 1105 118-119 0.14503910518971708 1105 120-121 -0.05052274072596674 1105 122-123 0.031399338166202995 1105 124-125 0.07470168633136254 1106 1 -0.010285245086542716 1106 2 0.058958875721785375 1106 3 0.026996202744079767 1106 4 0.008786937174640741 1106 5 -0.13574333619696688 1106 6 0.018693132684454383 1106 7 0.00486672814861322 1106 8 -0.02228356430612166 1106 9 6.047212991475703E-4 1106 10-11 0.06924512764435065 1106 12-13 0.0038629182642182514 1106 14-15 -0.0918646662491156 1106 16-17 -0.023100718805714848 1106 18-19 -0.0827975489928221 1106 20-21 0.021433225801175126 1106 22-23 0.0427549468178654 1106 24-25 0.06993732697638677 1106 26-27 0.1059799538333479 1106 28-29 0.06609479891475445 1106 30-31 0.025446013175240978 1106 32-33 0.09627588825653532 1106 34-35 0.10644154981726217 1106 36-37 0.16493731564600722 1106 38-39 0.11341660216562133 1106 40-41 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92-93 -0.48462421486449614 2216 94-95 -0.45439970692409304 2216 96-97 -0.5543499657746764 2216 98-99 -0.5103936046771835 2216 100-101 -0.5272330355730261 2216 102-103 -0.5025816550426221 2216 104-105 -0.5433780451815267 2216 106-107 -0.5784376268556386 2216 108-109 -0.5707908746067503 2216 110-111 -0.5146156984245636 2216 112-113 -0.5596581695796061 2216 114-115 -0.5579576452516406 2216 116-117 -0.6679218077445483 2216 118-119 -0.6902558514413393 2216 120-121 -0.6955063599401683 2216 122-123 -0.7177218386540432 2216 124-125 -0.827750862341265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 131.0 3 8.0 4 15.0 5 38.0 6 126.0 7 250.0 8 458.0 9 995.0 10 2000.0 11 3244.0 12 3748.0 13 6295.0 14 11194.0 15 12488.0 16 4141.0 17 2485.0 18 2096.0 19 1902.0 20 1942.0 21 2055.0 22 2153.0 23 2144.0 24 2653.0 25 3362.0 26 4235.0 27 5502.0 28 7111.0 29 9220.0 30 11800.0 31 15167.0 32 19780.0 33 26240.0 34 37976.0 35 60784.0 36 129244.0 37 460516.0 38 475659.0 39 5168.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.019665633263976 20.588499847088382 14.874102768599752 23.517731751047894 2 12.920390459595676 20.619489254866693 45.09036404174395 21.369756243793677 3 18.19744065351395 26.77601034230791 29.962715230547282 25.06383377363086 4 11.191501320892586 17.772131976842225 40.325258089296085 30.711108612969106 5 11.38247194923547 39.72620112503227 36.06601910273574 12.825307822996523 6 28.924812170947856 38.75959754932269 19.444737976130252 12.870852303599198 7 26.759522605062486 33.160886590598246 22.831394150600488 17.24819665373878 8 23.669495812489462 39.353680699979385 20.88861409326813 16.088209394263018 9 26.324321286043507 16.417889194911282 22.44213366308808 34.81565585595713 10-11 23.404118187098348 26.900904951941996 30.559421430311208 19.135555430648456 12-13 26.191407640567327 24.932906151050158 29.670095366571115 19.205590841811404 14-15 20.127030521049967 29.514436138122274 27.32864931707043 23.02988402375733 16-17 18.95457214493176 30.379866366839554 31.084530037483386 19.5810314507453 18-19 19.97545575478238 27.466134562419203 33.354242781930935 19.20416690086748 20-21 20.9818333621883 27.611992873947262 31.61593813494981 19.790235628914626 22-23 19.65470840423391 29.89984048072372 31.754753258491302 18.690697856551065 24-25 20.18863470293969 27.939632398403685 33.39917186592472 18.472561032731903 26-27 18.407359526352277 29.649223390103614 32.61458040582317 19.328836677720947 28-29 18.7747737620145 29.183707117831116 31.20379217956645 20.837726940587938 30-31 19.870721151143837 27.793566035261275 32.40597305753845 19.929739756056435 32-33 18.62151274989227 29.367845165158414 32.99106289697038 19.01957918797894 34-35 18.954190320948793 29.368707024150787 33.01512000449666 18.661982650403765 36-37 18.53412774249152 28.50014052048789 32.67344912221535 20.292282614805238 38-39 20.012365802934067 29.338092293856445 31.435332471474343 19.214209431735146 40-41 21.545762838888578 27.39145260712345 32.38487624829033 18.67790830569764 42-43 19.03010885653795 29.5564798680981 32.53457740805276 18.878833867311187 44-45 18.71710415378562 29.717010473460366 31.60650516178592 19.959380210968092 46-47 20.57812002323272 27.973207426976188 31.09703408090233 20.351638468888762 48-49 20.201000505873758 28.7770595619508 30.35939519982013 20.66254473235531 50-51 19.44353886796695 30.393157589043152 30.131077511101118 20.032226031888783 52-53 19.285968560882843 29.63449684297304 31.025087591104118 20.054447005040004 54-55 19.44477544826036 28.77912052910648 31.495100518989 20.281003503644165 56-57 19.9930676559309 29.062372360556836 29.405242351001444 21.53931763251082 58-59 20.43126674535814 29.36259906694396 29.04640923313286 21.159724954565043 60-61 19.713000955539318 28.179341614674087 31.427425852022555 20.680231577764037 62-63 20.22475783635921 28.247953084893112 31.22488898881457 20.302400089933112 64-65 20.460719839619284 28.95936147490304 29.808667303692875 20.771251381784797 66-67 20.49811702546231 30.106345905232985 29.132445243849887 20.26309182545482 68-69 21.275363948063628 28.103460551215033 31.021078073183073 19.60009742753827 70-71 21.70269611976093 28.27137316620763 30.194180578194967 19.831750135836472 72-73 20.77252543420831 29.414423022876733 29.60485638806138 20.208195154853577 74-75 19.747812564405223 29.814400539598672 31.016169224139546 19.421617671856556 76-77 20.348490809960094 28.236636501601936 31.42952429130834 19.985348397129634 78-79 19.855957131883162 28.250838438911057 30.64954190320949 21.24366252599629 80-81 20.33095385307178 29.2900904951942 30.357783898225694 20.02117175350833 82-83 19.882524872126357 29.257976879695725 31.22166638562569 19.637831862552225 84-85 19.729151443613812 28.66115826354149 31.288291832949245 20.321398459895455 86-87 18.83371742266689 30.333464485788696 29.650572386787328 21.182245704757086 88-89 20.21936184962434 28.409720270548778 30.529181421317897 20.841736458508983 90-91 20.081577591456274 29.064106047121648 31.149899292706916 19.704417068715163 92-93 19.20139396323984 30.158094916905554 30.968429730388024 19.672081389466584 94-95 19.188565223142504 29.925418688458617 30.49503659366919 20.390979494729695 96-97 20.635265021640155 29.023551233769883 30.06535139490004 20.275832349689917 98-99 20.53311059498569 28.580470223735123 31.044869186188123 19.84154999509106 100-101 19.823086314183296 28.972670035666027 31.01868887818017 20.185554771970505 102-103 19.350147416133183 29.742385248493985 30.537323186826526 20.3701441485463 104-105 19.88896737409526 28.842551389594114 30.36702971799662 20.901451518314012 106-107 20.006737978816215 28.333013547909353 29.990863630503934 21.6693848427705 108-109 19.50424093216195 29.514414672669737 29.577667983819445 21.403676411348872 110-111 19.270971714188175 29.516573100129623 30.377952348706827 20.83450283697538 112-113 19.6358351630767 28.33533265352699 30.104777869343017 21.92405431405329 114-115 19.492008458656127 29.02152475408552 28.94459040610318 22.54187638115517 116-117 19.964708139536626 28.894395721508324 28.13291143983896 23.00798469911609 118-119 20.123583316626977 28.035763467977194 29.59280833531624 22.24784488007959 120-121 19.357766399462793 28.179316381775777 30.3900062354232 22.07291098333823 122-123 20.159648476568165 28.8778852535006 29.20198620032204 21.760480069609194 124-125 20.564661638075595 28.77171498088703 28.3990861149656 22.264537266071773 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 218.0 1 145.0 2 145.0 3 204.5 4 120.0 5 83.0 6 239.5 7 596.5 8 1014.0 9 1064.0 10 743.0 11 550.0 12 577.0 13 669.0 14 824.5 15 967.5 16 1143.0 17 1369.5 18 1630.0 19 1938.0 20 2321.5 21 2908.0 22 3698.0 23 4709.0 24 5822.5 25 7176.0 26 9181.5 27 11856.5 28 14621.5 29 17690.0 30 21777.0 31 27121.0 32 34639.5 33 44643.5 34 56391.0 35 68693.5 36 78684.5 37 81270.0 38 75277.5 39 65837.5 40 58063.0 41 52930.0 42 48744.0 43 45845.5 44 44011.5 45 42490.5 46 40882.0 47 39103.0 48 38654.0 49 37501.5 50 35195.0 51 32439.5 52 29115.0 53 25888.5 54 22330.5 55 18868.0 56 15695.5 57 12924.0 58 10566.5 59 8490.5 60 6664.5 61 5058.5 62 3933.0 63 2931.0 64 2087.5 65 1580.0 66 1077.0 67 801.5 68 605.5 69 376.0 70 240.0 71 186.0 72 159.5 73 142.5 74 111.5 75 78.0 76 41.5 77 20.5 78 14.5 79 5.0 80 4.0 81 4.0 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 1.0 98 4.5 99 5.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016562681505630186 2 0.0 3 0.0 4 0.0 5 0.13047795701946677 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.7472130103235724E-5 18-19 0.0 20-21 0.0049463211736271145 22-23 2.6230491072265006E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 9.368032525808929E-4 92-93 0.0 94-95 7.119704719614786E-4 96-97 0.0 98-99 0.0017986622449553144 100-101 1.498885204129429E-4 102-103 5.246098214453001E-4 104-105 0.0024356884567103216 106-107 0.007269593240027729 108-109 5.620819515485358E-4 110-111 0.005208626084349765 112-113 0.00910572761508628 114-115 0.005283570344556236 116-117 0.001723717984748843 118-119 1.873606505161786E-4 120-121 9.742753826841287E-4 122-123 0.0013864688138197215 124-125 0.0016113015944391358 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1334325.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.2674385843719 #Duplication Level Percentage of deduplicated Percentage of total 1 77.52373273359039 49.82251732287282 2 13.852028183271099 17.804687410747277 3 4.297247528253988 8.285192748117213 4 1.795873370680124 4.616647262219753 5 0.8860057756207745 2.847066088505346 6 0.44828847110646997 1.7286211070950224 7 0.28765863060892277 1.2940958363146429 8 0.19710192917419753 1.0133788902451168 9 0.13118202732344142 0.7587659595944402 >10 0.528911102286781 5.741169341804317 >50 0.031648201909420144 1.419159370028216 >100 0.016701496522424898 2.0062745527461034 >500 0.0025694223336660623 1.0440731686747287 >1k 0.0010511273183179347 1.6183509410349264 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4230 0.31701422067337415 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 3468 0.25990669439604297 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3133 0.23480036722687503 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 2683 0.20107545013396286 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2041 0.15296123508140821 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 1961 0.14696569426489048 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 1694 0.12695557678976263 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.494426020647143E-5 0.0 14-15 0.0 0.0 0.0 1.1241639030970715E-4 0.0 16-17 0.0 0.0 0.0 1.873606505161786E-4 0.0 18-19 0.0 0.0 0.0 2.9977704082588574E-4 0.0 20-21 0.0 0.0 0.0 3.747213010323572E-4 0.0 22-23 0.0 0.0 0.0 4.8713769134206433E-4 0.0 24-25 0.0 0.0 0.0 6.744983418582429E-4 0.0 26-27 0.0 0.0 0.0 0.001199108163303543 0.0 28-29 0.0 0.0 0.0 0.0023232720664006145 0.0 30-31 0.0 0.0 0.0 0.006033012946620951 0.0 32-33 0.0 0.0 0.0 0.013527438967268095 0.0 34-35 0.0 0.0 0.0 0.02195866824049613 0.0 36-37 0.0 0.0 0.0 0.03758454649354542 0.0 38-39 0.0 0.0 0.0 0.06366514904539748 0.0 40-41 0.0 0.0 0.0 0.09510426620201226 0.0 42-43 0.0 0.0 0.0 0.12710546531017555 0.0 44-45 0.0 0.0 0.0 0.1652520937552695 0.0 46-47 0.0 0.0 0.0 0.20984392857812 0.0 48-49 0.0 0.0 0.0 0.2575084780694359 0.0 50-51 0.0 0.0 0.0 0.3097820995634497 0.0 52-53 0.0 0.0 0.0 0.36426657673355445 0.0 54-55 0.0 0.0 0.0 0.42242332265377625 0.0 56-57 0.0 0.0 0.0 0.48972326831918767 0.0 58-59 0.0 0.0 0.0 0.5686021021864989 0.0 60-61 0.0 0.0 0.0 0.657973132482716 0.0 62-63 0.0 0.0 0.0 0.7495550184550241 0.0 64-65 0.0 0.0 0.0 0.8451838944784816 0.0 66-67 0.0 0.0 0.0 0.949843553856819 0.0 68-69 0.0 0.0 0.0 1.0805838157870085 0.0 70-71 0.0 0.0 0.0 1.2331703295673844 0.0 72-73 0.0 0.0 0.0 1.3721919322503888 0.0 74-75 0.0 0.0 0.0 1.5157851348059879 0.0 76-77 0.0 0.0 0.0 1.6664230978209957 0.0 78-79 0.0 0.0 0.0 1.841380473273003 0.0 80-81 0.0 0.0 0.0 2.036760159631274 0.0 82-83 0.0 0.0 0.0 2.2368238622524497 0.0 84-85 0.0 0.0 0.0 2.4342270436362954 0.0 86-87 0.0 0.0 0.0 2.6450077754669965 0.0 88-89 0.0 0.0 0.0 2.871189552770127 0.0 90-91 0.0 0.0 0.0 3.1221404080714965 0.0 92-93 0.0 0.0 0.0 3.390141082569839 0.0 94-95 0.0 0.0 0.0 3.656830232514567 0.0 96-97 0.0 0.0 0.0 3.935735296872951 0.0 98-99 0.0 0.0 0.0 4.246379255428774 0.0 100-101 0.0 0.0 0.0 4.581604931332322 0.0 102-103 0.0 0.0 0.0 4.928709272478594 0.0 104-105 0.0 0.0 0.0 5.284020010117475 0.0 106-107 0.0 0.0 0.0 5.643003016506473 0.0 108-109 0.0 0.0 0.0 6.022071084630806 0.0 110-111 0.0 0.0 0.0 6.437037453394038 0.0 112-113 0.0 0.0 0.0 6.900830007681787 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5230 0.0 57.531933 1 GGTATCA 2930 0.0 55.922215 1 ATCAACG 6520 0.0 45.778393 3 TCAACGC 6640 0.0 44.951073 4 CAACGCA 6765 0.0 44.208553 5 TATCAAC 6825 0.0 43.9072 2 AACGCAG 7010 0.0 42.69078 6 CGCAGAG 8085 0.0 36.867344 8 ACGCAGA 8105 0.0 36.849777 7 TAGGACC 2450 0.0 35.988686 4 TTCGCGT 35 0.0027906771 33.997433 26-27 GTACATG 6525 0.0 32.5992 1 GCAGAGT 9215 0.0 32.28188 9 TACATGG 6610 0.0 31.36521 2 ATGGGCG 155 1.94932E-7 30.748838 5 ACATGGG 6425 0.0 30.228382 3 TTACGCA 80 0.0053292047 29.787937 3 CTACAGT 6685 0.0 29.320063 4 TACAGTG 6960 0.0 28.931961 5 CGGGAGT 250 2.1827873E-11 28.596418 4 >>END_MODULE