FastQCFastQC Report
Fri 27 May 2016
SRR939904_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939904_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555615
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA19730.12683086753470493No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC16190.10407459429228955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC3800.01929.6838108-109
CCGCTCG1250.01618.2728108-109
CCGCGCC3000.01517.1307108-109
CGTATCG200.00366089351493.8862110-111
CCGTTCG551.508306E-81379.2098108-109
CTCCGCC3600.01327.8988110-111
CCGCGCA502.0962683E-51011.4205108-109
CGATTGT300.008235182995.9242110-111
CGTGTCG602.1933276E-5995.9242110-111
CCGCGCG2500.0910.27844108-109
TTGTTCG2000.0884.9929108-109
CAGCGCG1203.2196112E-10842.8504108-109
CCGCACC2500.0809.1364108-109
CGTCGCC2450.0722.4432108-109
CCTCGCG2005.456968E-12632.1378108-109
CCGTACG405.865215E-6632.1378108-109
CATCGCC2400.0632.1378108-109
GGTCGCC2107.2759576E-12602.036108-109
CGCCGTC1001.01441736E-4597.55444110-111
CCTCTCG3000.0589.99524108-109
Produced by FastQC (version 0.11.5)