##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939904_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1555615 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.933786316022925 34.0 31.0 34.0 30.0 34.0 2 32.00807269150786 34.0 31.0 34.0 30.0 34.0 3 32.09090488327767 34.0 31.0 34.0 30.0 34.0 4 35.34762714424841 37.0 35.0 37.0 33.0 37.0 5 35.339211822976765 37.0 35.0 37.0 33.0 37.0 6 35.43357964534927 37.0 37.0 37.0 33.0 37.0 7 35.3472909428104 37.0 37.0 37.0 33.0 37.0 8 35.37608148545752 37.0 37.0 37.0 33.0 37.0 9 37.063410291106734 39.0 38.0 39.0 35.0 39.0 10-11 37.05651398321564 39.0 38.0 39.0 34.5 39.0 12-13 37.03473577973985 39.0 38.0 39.0 35.0 39.0 14-15 38.569467702484225 41.0 39.5 41.0 35.0 41.0 16-17 38.57049012769869 41.0 39.0 41.0 35.0 41.0 18-19 38.57859753216573 41.0 39.5 41.0 35.0 41.0 20-21 38.623672952497884 41.0 39.5 41.0 35.0 41.0 22-23 38.604649929449124 41.0 39.0 41.0 34.0 41.0 24-25 38.628625656091 41.0 39.0 41.0 34.0 41.0 26-27 38.59465420428577 41.0 39.0 41.0 34.0 41.0 28-29 38.54108085869576 41.0 39.0 41.0 34.0 41.0 30-31 38.47415523763913 41.0 39.0 41.0 34.0 41.0 32-33 38.376651999370026 40.5 39.0 41.0 34.0 41.0 34-35 38.31464822594279 40.0 39.0 41.0 34.0 41.0 36-37 38.489840352529384 41.0 39.0 41.0 34.0 41.0 38-39 38.46310076722068 41.0 39.0 41.0 34.0 41.0 40-41 38.39871915608939 41.0 39.0 41.0 34.0 41.0 42-43 38.33064190047023 41.0 39.0 41.0 33.5 41.0 44-45 38.24667832336407 41.0 39.0 41.0 33.0 41.0 46-47 38.15821781096223 41.0 38.5 41.0 33.0 41.0 48-49 38.0745785428914 41.0 38.0 41.0 33.0 41.0 50-51 37.965637063155086 40.0 38.0 41.0 33.0 41.0 52-53 37.86098134821276 40.0 38.0 41.0 33.0 41.0 54-55 37.7199663798562 40.0 38.0 41.0 33.0 41.0 56-57 37.55340878045017 40.0 37.5 41.0 32.0 41.0 58-59 37.39702304233374 40.0 37.0 41.0 32.0 41.0 60-61 37.19210151612063 40.0 37.0 41.0 32.0 41.0 62-63 36.94819765816092 40.0 36.0 41.0 31.0 41.0 64-65 36.66893125869833 39.0 36.0 41.0 31.0 41.0 66-67 36.376553324569386 39.0 35.0 41.0 31.0 41.0 68-69 36.004350048051734 39.0 35.0 41.0 30.5 41.0 70-71 35.608808091976485 37.5 35.0 40.0 30.0 41.0 72-73 35.22107012339171 37.0 35.0 39.5 30.0 41.0 74-75 34.794311895938264 37.0 35.0 39.0 30.0 41.0 76-77 34.34091275797675 36.0 34.5 39.0 29.0 40.5 78-79 33.88665897410349 36.0 34.0 37.5 29.0 39.5 80-81 33.45705074841783 35.0 34.0 37.0 29.0 39.0 82-83 33.2447973952424 35.0 34.0 37.0 29.0 39.0 84-85 32.82823063547214 35.0 34.0 36.5 29.0 38.0 86-87 32.1991042128033 35.0 34.0 36.0 27.5 37.0 88-89 16.583261603931565 18.0 17.0 18.5 2.0 19.0 90-91 2.8153453778730597 2.0 2.0 2.0 2.0 2.0 92-93 2.307423430604616 2.0 2.0 2.0 2.0 2.0 94-95 2.103073382552881 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.07960738413381918 1101 2 0.08837706342421114 1101 3 0.08233606498334467 1101 4 0.14264859042617672 1101 5 0.07741486954195409 1101 6 0.05781766201222638 1101 7 0.13281804749482973 1101 8 0.06904109851756601 1101 9 0.11479492867451313 1101 10-11 0.1514126869184409 1101 12-13 0.18255741094083078 1101 14-15 0.2087881717312996 1101 16-17 0.27253297050511804 1101 18-19 0.15882593804108325 1101 20-21 0.1543776262929768 1101 22-23 0.22110982126690715 1101 24-25 0.1575545373804914 1101 26-27 0.12994057891832256 1101 28-29 0.21665056456448895 1101 30-31 0.14842802860869142 1101 32-33 0.12665063226326367 1101 34-35 0.1321644854819084 1101 36-37 0.08317774591409233 1101 38-39 0.15511038859430926 1101 40-41 0.12708490248262194 1101 42-43 0.13700550918190402 1101 44-45 0.17861956474386176 1101 46-47 0.11624786736377501 1101 48-49 0.1637643281758372 1101 50-51 0.04289269945135743 1101 52-53 0.050317635422644 1101 54-55 0.04572719937184644 1101 56-57 0.07749141970315065 1101 58-59 0.06272246398412307 1101 60-61 0.10960749986527674 1101 62-63 0.08952111490579284 1101 64-65 0.09255460964794793 1101 66-67 0.04744601902981316 1101 68-69 0.0630786233503855 1101 70-71 0.029502344927941238 1101 72-73 0.06755542849483476 1101 74-75 0.05799733738433588 1101 76-77 0.05703009384343005 1101 78-79 0.01175577527848759 1101 80-81 -0.009229181657225638 1101 82-83 0.05726672222493079 1101 84-85 0.058635456375462525 1101 86-87 0.08713019384828868 1101 88-89 0.4475907068741165 1101 90-91 -0.13646803929100804 1101 92-93 -0.17118332251602109 1101 94-95 -0.010516534224112473 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.18363316658347983 1102 2 -0.13598369326055249 1102 3 -0.029615979366326428 1102 4 -0.0840996191372696 1102 5 -0.06709431546594402 1102 6 -0.0036020385262673926 1102 7 -0.03971160734419499 1102 8 0.06773274576002564 1102 9 0.07497111228565956 1102 10-11 0.023380499276690614 1102 12-13 0.058689333074141814 1102 14-15 0.056165423582157814 1102 16-17 0.061743439123638666 1102 18-19 -0.009880525903376736 1102 20-21 0.09242970563276032 1102 22-23 0.05889306463440391 1102 24-25 0.10330536119974454 1102 26-27 0.07983867071164497 1102 28-29 0.003996186492386755 1102 30-31 0.021561395666452654 1102 32-33 0.12670074938012021 1102 34-35 -0.031727979312599075 1102 36-37 0.0810214850307105 1102 38-39 -0.001719747654831849 1102 40-41 -0.030676840539662464 1102 42-43 -0.08368332660257494 1102 44-45 -0.04788306073981374 1102 46-47 -0.07402161370053051 1102 48-49 -0.008647740270873783 1102 50-51 -0.07676962345965421 1102 52-53 -0.10036585589699598 1102 54-55 -0.12147330135019985 1102 56-57 -0.1441537241625781 1102 58-59 -0.18552894878779824 1102 60-61 -0.16100941632542742 1102 62-63 -0.061601896383727706 1102 64-65 -0.11089648972357224 1102 66-67 -0.2308005392205743 1102 68-69 -0.13796246533629386 1102 70-71 -0.10382980437574219 1102 72-73 -0.13730293093818347 1102 74-75 -0.09815790196725516 1102 76-77 -0.18770873167265023 1102 78-79 -0.12749458289048476 1102 80-81 -0.181089451460835 1102 82-83 -0.17290670137229114 1102 84-85 -0.302200844176987 1102 86-87 -0.25915693415715424 1102 88-89 0.5940482330768972 1102 90-91 0.061663194471965 1102 92-93 0.10832586045044623 1102 94-95 0.18062075891263918 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.30262961103355934 1103 2 -0.10473993210984744 1103 3 -0.1853669704520975 1103 4 -0.3085389545473163 1103 5 -0.27686265015663736 1103 6 -0.36608433207779 1103 7 -0.3149003176809586 1103 8 -0.2765233932676807 1103 9 -0.3050433380776596 1103 10-11 -0.3310061445947383 1103 12-13 -0.23699785934068274 1103 14-15 -0.31917840633452954 1103 16-17 -0.2644505379902853 1103 18-19 -0.2697347388497704 1103 20-21 -0.2436174941339999 1103 22-23 -0.24419063022209286 1103 24-25 -0.3210522551874817 1103 26-27 -0.2339277166658391 1103 28-29 -0.2840827787221656 1103 30-31 -0.22954654474480662 1103 32-33 -0.19114267649680272 1103 34-35 -0.1503079063002417 1103 36-37 -0.19039519721657427 1103 38-39 -0.23990475350153417 1103 40-41 -0.203381855069253 1103 42-43 -0.23470744856871306 1103 44-45 -0.23377482403271443 1103 46-47 -0.2613673259671785 1103 48-49 -0.3147458886596226 1103 50-51 -0.35868836868644394 1103 52-53 -0.27541568746786993 1103 54-55 -0.376006072936228 1103 56-57 -0.3177803058594222 1103 58-59 -0.2971383976141837 1103 60-61 -0.369211106637664 1103 62-63 -0.41514144316202106 1103 64-65 -0.3651666354630976 1103 66-67 -0.3000943490144934 1103 68-69 -0.21724279129339408 1103 70-71 -0.18968112408052917 1103 72-73 -0.1478291570800394 1103 74-75 -0.24312538864978706 1103 76-77 -0.2094104204004239 1103 78-79 -0.2825343451566624 1103 80-81 -0.2538423565859631 1103 82-83 -0.28061280918594633 1103 84-85 -0.24994562889509808 1103 86-87 -0.15990634869561404 1103 88-89 0.5727598928722486 1103 90-91 -0.5621066628940765 1103 92-93 -0.3284506310900701 1103 94-95 -0.11368022060650596 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.13407693644355234 1104 2 -0.08212094323007335 1104 3 -0.013140705009909937 1104 4 0.007872475292884928 1104 5 -0.010480670719495322 1104 6 -0.041585605692077365 1104 7 -0.018199842157173407 1104 8 0.05667476631341373 1104 9 0.02191781206080634 1104 10-11 0.08555708588318822 1104 12-13 0.10273219919006493 1104 14-15 0.08716160242349957 1104 16-17 0.10818803309466318 1104 18-19 0.0775456474901759 1104 20-21 0.009909230179388828 1104 22-23 -0.06861842893552961 1104 24-25 -0.05576464347639387 1104 26-27 0.07323035027754798 1104 28-29 0.040977586894570095 1104 30-31 0.023853357806736142 1104 32-33 0.0054387638900337265 1104 34-35 0.04621918190294849 1104 36-37 0.014953134749603691 1104 38-39 -0.09038382367058517 1104 40-41 0.09000467915797117 1104 42-43 -0.0126560352577485 1104 44-45 0.07086685245075586 1104 46-47 0.03743939304205668 1104 48-49 0.08304733558900779 1104 50-51 -0.014747750567195794 1104 52-53 0.05805153469610502 1104 54-55 0.03889074271966564 1104 56-57 0.002861342869735495 1104 58-59 0.02164641091193431 1104 60-61 0.09542065539073974 1104 62-63 0.03980021111799914 1104 64-65 -0.07735139279794367 1104 66-67 0.05713402883880292 1104 68-69 0.145231921449529 1104 70-71 0.09859360654851201 1104 72-73 0.03750138129183256 1104 74-75 -0.010417092579189102 1104 76-77 -0.038812407608766364 1104 78-79 -0.12463970975572636 1104 80-81 0.022981409735116642 1104 82-83 0.04879033445184433 1104 84-85 0.11896962487177376 1104 86-87 -0.025372358921536886 1104 88-89 0.7376609487694203 1104 90-91 -0.5927015456167117 1104 92-93 -0.3284506310900701 1104 94-95 -0.11368022060650596 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.03146827123019591 1105 2 -0.09062185819187718 1105 3 -0.0391532515679458 1105 4 0.08313078349610947 1105 5 -0.09014900544432436 1105 6 -0.007829771274472819 1105 7 0.026347290850786464 1105 8 -0.06675626224963338 1105 9 0.061204193988281475 1105 10-11 0.02487271903154209 1105 12-13 0.0867104992900849 1105 14-15 -0.05365565546166806 1105 16-17 0.08924474881003874 1105 18-19 0.10564720787802884 1105 20-21 0.05500759322818283 1105 22-23 -0.07194144955325754 1105 24-25 0.033488074410755075 1105 26-27 0.0160024126990308 1105 28-29 -0.013241161592603135 1105 30-31 -0.05906659178657492 1105 32-33 -0.10921969133269727 1105 34-35 -0.008303343585275513 1105 36-37 -0.04912910307492524 1105 38-39 -0.013780629608561412 1105 40-41 0.005848581535232711 1105 42-43 0.0162994150025213 1105 44-45 -0.0679001201465681 1105 46-47 -0.08356309526676853 1105 48-49 -0.09820503374127298 1105 50-51 -0.09374866962529183 1105 52-53 -0.07253468509284033 1105 54-55 -0.05773529373431785 1105 56-57 -0.1252059390345721 1105 58-59 -0.17440761614889055 1105 60-61 -0.21731139001995814 1105 62-63 -0.17898672529792492 1105 64-65 -0.15366449518504055 1105 66-67 -0.1680741184547756 1105 68-69 -0.18276177622504264 1105 70-71 -0.35490309668436026 1105 72-73 -0.32342228326238853 1105 74-75 -0.3414625248828074 1105 76-77 -0.24123625575118268 1105 78-79 -0.17173184793618645 1105 80-81 -0.38229937591804486 1105 82-83 -0.2811984417187787 1105 84-85 -0.23676301859698867 1105 86-87 -0.3108671607319451 1105 88-89 0.7627522959938879 1105 90-91 -0.8039375335185115 1105 92-93 -0.3192112671021028 1105 94-95 -0.10766389056782977 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.30756208894625914 1106 2 -0.30934476037676006 1106 3 -0.2633652439150431 1106 4 -0.27291177185131943 1106 5 -0.26824618889354923 1106 6 -0.27381873513967747 1106 7 -0.3268008214554996 1106 8 -0.19508185556306756 1106 9 -0.2555034964240406 1106 10-11 -0.41322926479733724 1106 12-13 -0.29307880887569837 1106 14-15 -0.2777124646926836 1106 16-17 -0.3587007470159662 1106 18-19 -0.3294425103794154 1106 20-21 -0.3436643928569083 1106 22-23 -0.3790204968984199 1106 24-25 -0.3642652968809301 1106 26-27 -0.49900907066931666 1106 28-29 -0.3292034627568299 1106 30-31 -0.2564184187921228 1106 32-33 -0.23533198887021456 1106 34-35 -0.3995353790665348 1106 36-37 -0.3009137544715017 1106 38-39 -0.2795543600502981 1106 40-41 -0.23105379944578175 1106 42-43 -0.5941263571678945 1106 44-45 -0.3394616431900346 1106 46-47 -0.39445292236197815 1106 48-49 -0.3212362493308021 1106 50-51 -0.3162467223186667 1106 52-53 -0.3534079240879606 1106 54-55 -0.35286496777140286 1106 56-57 -0.5309234937350311 1106 58-59 -0.35995231344248424 1106 60-61 -0.3039671729101201 1106 62-63 -0.29034933693773013 1106 64-65 -0.3000653221479297 1106 66-67 -0.35198761612366525 1106 68-69 -0.27979523636567194 1106 70-71 -0.19804139682508293 1106 72-73 -0.19651648333215377 1106 74-75 -0.2856756981889106 1106 76-77 -0.300720686957213 1106 78-79 -0.23633495276489214 1106 80-81 -0.13813683076185157 1106 82-83 -0.1247330374389719 1106 84-85 -0.15236855350398315 1106 86-87 -0.09802135206115992 1106 88-89 0.8345289066634152 1106 90-91 -0.24713231193014362 1106 92-93 -0.2979162799450319 1106 94-95 -0.11368022060650596 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.04039465835441902 1107 2 -0.052637228315269624 1107 3 0.007271709673140947 1107 4 0.005018932559906375 1107 5 -0.032011355888016624 1107 6 -0.11817411398001099 1107 7 -0.1139255317090857 1107 8 -0.16539957480392786 1107 9 -0.07963503598460875 1107 10-11 -0.07682323892159104 1107 12-13 -0.050184056383209 1107 14-15 -0.1168186014402437 1107 16-17 -0.04042874009462594 1107 18-19 -0.07558182087853282 1107 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98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16607.0 3 2739.0 4 1855.0 5 1659.0 6 1689.0 7 1843.0 8 2079.0 9 2407.0 10 2781.0 11 3449.0 12 4180.0 13 4823.0 14 5404.0 15 5973.0 16 6728.0 17 8249.0 18 10681.0 19 13180.0 20 13767.0 21 19324.0 22 22185.0 23 28577.0 24 43743.0 25 75108.0 26 150419.0 27 451499.0 28 630548.0 29 23295.0 30 824.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.544855250174365 19.909746306123303 14.494267540490416 24.051130903211913 2 13.529016232839552 20.164861160752167 43.579890101130616 22.726232505277665 3 18.63831943559335 25.363573752338354 29.09226654594164 26.905840266126663 4 11.7253371901815 17.488101156089808 39.52564111633375 31.26092053739494 5 12.902288113629451 38.74988826000081 35.271703444936136 13.076120181433604 6 30.417551900695223 37.697502274020245 19.044429373591797 12.840516451692737 7 28.114025642591518 32.01029817789106 22.148089340871614 17.72758683864581 8 24.119464006196907 38.48439363210049 20.685773793644312 16.710368568058293 9 25.52881014904073 17.763714029499585 21.300257454447276 35.407218367012405 10-11 23.885513279729288 27.252501279245877 29.38408489564182 19.477900545383015 12-13 25.48709026385174 24.611923646951848 28.79130387547615 21.109682213720266 14-15 20.90289049668459 28.2261035024733 25.924666450246363 24.946339550595745 16-17 20.841933930698524 30.02354704813356 28.43355881844345 20.700960202724463 18-19 21.47491798546721 28.673135684148743 29.279364995689537 20.572581334694508 20-21 22.347073510655804 28.362338517238545 28.09385414095285 21.1967338311528 22-23 21.465775567278744 29.464970736494095 28.057834677342893 21.011419018884276 24-25 21.72391626462846 28.482947258801182 28.929683758513512 20.863452718056845 26-27 20.701233917132452 29.51880767413531 28.548934022878413 21.231024385853825 28-29 20.849076222529426 29.379083843400267 27.811742955194358 21.96009697887595 30-31 21.567418673643544 28.476679641170854 28.409792911485166 21.546108773700436 32-33 20.865165957132227 29.238443749106054 28.70308675605354 21.19330353770818 34-35 21.21600256104746 29.421125491646865 28.59293134187894 20.769940605426733 36-37 20.969990296432886 28.74174722705865 28.557993357604573 21.73026911890389 38-39 21.467618915991423 29.256017716465838 28.083073253986367 21.193290113556376 40-41 22.441992395290608 28.069027362168658 28.65307290042845 20.835907342112282 42-43 20.957981248573716 29.252932120093984 28.703085274955566 21.086001356376737 44-45 20.832578004569257 29.30788100389943 28.314029058622513 21.545511932908806 46-47 21.662782886511124 28.403943135030197 28.24358854858047 21.689685429878217 48-49 21.395867289657748 28.6421723647937 28.203438707622425 21.75852163792613 50-51 20.95020970812499 29.45041975373719 28.19557801756155 21.403792520576275 52-53 21.06661995416604 29.023023048762063 28.57384378525535 21.33651321181655 54-55 20.991383993113878 28.587747566362154 28.803035241223125 21.617833199300847 56-57 21.32188877067912 28.724588024671917 27.857856860469976 22.09566634417899 58-59 21.586414376307765 28.850068943793932 27.552639952687525 22.010876727210782 60-61 21.171176672891427 28.21585032286266 28.930005174802247 21.682967829443662 62-63 21.456401487514583 28.29540085432449 28.783857188314588 21.46434046984633 64-65 21.520980937115205 28.566042551030478 28.013270639994676 21.89970587185964 66-67 21.43763077625248 29.31400121495357 27.581053152611666 21.667314856182283 68-69 21.91420790371877 28.13305652864681 28.762423988796677 21.190311578837747 70-71 22.24618559219344 28.114604191911237 28.256252350356608 21.38295786553871 72-73 21.747508220221583 28.838208682739623 27.955760262018558 21.458522835020233 74-75 21.16044136884769 29.193470106678067 28.58554333816529 21.06054518630895 76-77 21.526502380087617 28.202897246426655 28.837308717131165 21.433291656354562 78-79 21.342445305986274 28.306213193675273 28.45792226709925 21.8934192332392 80-81 21.632151914194708 28.734037663560713 28.280133580609597 21.35367684163498 82-83 21.388201231291017 28.83402770501214 28.725195310114472 21.052575753582374 84-85 21.362322939801942 28.530227594874052 28.726645088919817 21.380804376404186 86-87 20.81180356364562 29.33145324177583 27.923377376417303 21.933365818161246 88-89 30.59887880140894 17.89925088058739 34.58685320236146 16.915017115642208 90-91 31.148428776137145 14.836516648084805 16.732040837399342 37.28301373837871 92-93 22.858215500529155 17.462773032820575 35.07075362290698 24.608257843743292 94-95 15.917660056986685 7.0230272721986395 27.61342094551375 49.445891725300925 96-97 27.074544220605635 7.175428494726846 14.95845830965526 50.79156897501226 98-99 57.19775625064597 14.153097370077706 18.83932011012036 9.809826269155963 100-101 30.9295114558463 7.14483927228464 12.33921531038902 49.58643396148004 102-103 23.668729013439 2.085266855417582 37.004140127168455 37.24186400397497 104-105 42.53564120884327 4.977348913797828 39.479985351700044 13.007024525658853 106-107 5.895379583884211 1.7497042655347577 6.866780321480761 85.48813582910027 108-109 18.465860515056967 10.858924694016006 21.22507588697208 49.45013890395495 110-111 40.762283259748195 2.7067994348325386 24.490992381156325 32.03992492426294 112-113 28.04684698986363 2.8427440104343593 38.456519355561284 30.65388964414073 114-115 29.682634093502774 4.111298090872164 23.32540281243351 42.88066500319155 116-117 20.250647979264663 4.279159066909859 20.30555822213689 55.16463473168859 118-119 20.460333634166933 4.2171824507263675 15.30687938513494 60.01560452997175 120-121 26.0167609911649 4.356276477292942 29.104268650226313 40.52269388131584 122-123 34.82760292774651 1.6490798934287338 33.41222013593494 30.111097042889817 124-125 39.08197857381123 1.9389847513595002 19.39139901169571 39.58763766313356 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 19.0 1 53.5 2 106.0 3 165.0 4 223.0 5 245.0 6 226.0 7 175.5 8 148.0 9 142.5 10 142.5 11 151.0 12 156.5 13 155.0 14 172.5 15 199.0 16 254.5 17 372.5 18 519.0 19 760.0 20 1128.0 21 1728.5 22 2639.5 23 3865.5 24 5502.5 25 7536.0 26 9970.0 27 13097.0 28 16771.5 29 21069.0 30 26269.5 31 32309.0 32 39176.0 33 46425.5 34 53455.0 35 59568.0 36 63510.5 37 65006.5 38 65684.5 39 66337.5 40 66709.0 41 66273.0 42 65041.5 43 63753.5 44 62686.5 45 61431.0 46 59406.0 47 57116.0 48 54491.5 49 51086.0 50 47998.5 51 44675.0 52 40197.5 53 35676.5 54 31323.0 55 27002.5 56 23027.5 57 19404.5 58 16069.0 59 13098.5 60 10456.0 61 8180.5 62 6367.5 63 4876.5 64 3631.5 65 2645.5 66 1949.0 67 1473.5 68 1104.0 69 824.0 70 613.0 71 438.0 72 301.5 73 209.5 74 171.0 75 137.5 76 96.5 77 59.5 78 32.5 79 22.5 80 17.5 81 15.5 82 10.5 83 8.0 84 7.5 85 9.0 86 8.0 87 5.0 88 5.5 89 7.0 90 5.0 91 4.0 92 4.5 93 3.0 94 3.5 95 4.5 96 4.5 97 3.5 98 4.0 99 3.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 1.928497732408083E-4 3 0.03638432388476583 4 0.04062701889606362 5 0.04281264965945944 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.071158352162971E-4 12-13 0.0016392230725468704 14-15 0.0 16-17 3.856995464816166E-4 18-19 0.0062997592591997375 20-21 0.001992780990155019 22-23 5.464076908489569E-4 24-25 0.0 26-27 0.0 28-29 0.001189240268318318 30-31 0.0 32-33 0.0011249570105713817 34-35 9.642488662040415E-5 36-37 9.642488662040415E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 6.428325774693609E-5 46-47 0.0 48-49 9.642488662040415E-5 50-51 3.2141628873468047E-5 52-53 0.0 54-55 0.0014142316704325942 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.2141628873468047E-5 66-67 0.0 68-69 3.5355791760814854E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.856995464816166E-4 80-81 0.0 82-83 7.713990929632332E-4 84-85 0.0 86-87 0.0010928153816979137 88-89 19.015148349688065 90-91 26.226958469801332 92-93 40.41269851473533 94-95 44.726040826296995 96-97 48.75653680377214 98-99 93.15839716125134 100-101 52.047839600415266 102-103 53.48923094724595 104-105 74.89809496565667 106-107 81.52370605837562 108-109 12.59067957045927 110-111 38.76132590647428 112-113 52.21738669272281 114-115 86.7064794309646 116-117 66.51806520250834 118-119 72.39917974563114 120-121 80.48604571182459 122-123 76.1376047415331 124-125 50.28037142866326 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1555615.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.15380136022708 #Duplication Level Percentage of deduplicated Percentage of total 1 78.5166706596475 51.9417623428469 2 13.092315301573498 17.322128516115093 3 4.106349161887711 8.149518203137635 4 1.708429294589002 4.520763687689348 5 0.8622701267539638 2.8521223342069764 6 0.4807038630637989 1.908023272212981 7 0.2879975306157155 1.333649200281157 8 0.19266844990024232 1.0196600290466793 9 0.12690868685319032 0.7555942854875891 >10 0.5829205180194397 6.617324311882039 >50 0.028670446209830714 1.2874588396919102 >100 0.013318311097320205 1.7431465783823459 >500 4.860311180007633E-4 0.24789813453071027 >1k 2.91618670800458E-4 0.30095026448864437 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1973 0.12683086753470493 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1619 0.10407459429228955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.2141628873468047E-5 0.0 18-19 0.0 0.0 0.0 1.2856651549387219E-4 0.0 20-21 0.0 0.0 0.0 2.571330309877444E-4 0.0 22-23 0.0 0.0 0.0 3.5355791760814854E-4 0.0 24-25 0.0 0.0 0.0 3.856995464816166E-4 0.0 26-27 0.0 0.0 0.0 5.464076908489569E-4 0.0 28-29 0.0 0.0 0.0 0.0015427981859264662 0.0 30-31 0.0 0.0 0.0 0.003985561980310038 0.0 32-33 0.0 0.0 0.0 0.009031797713444522 0.0 34-35 0.0 0.0 0.0 0.01501014068390958 0.0 36-37 0.0 0.0 0.0 0.024459779572709187 0.0 38-39 0.0 0.0 0.0 0.04204125056649621 0.0 40-41 0.0 0.0 0.0 0.06235476001452801 0.0 42-43 0.0 0.0 0.0 0.08366465995763733 0.0 44-45 0.0 0.0 0.0 0.10709590740639555 0.0 46-47 0.0 0.0 0.0 0.13653763945449227 0.0 48-49 0.0 0.0 0.0 0.16912925113218888 0.0 50-51 0.0 0.0 0.0 0.20577070804794245 0.0 52-53 0.0 0.0 0.0 0.24636558531513258 0.0 54-55 0.0 0.0 0.0 0.29039961687178384 0.0 56-57 0.0 0.0 0.0 0.3424690556468021 0.0 58-59 0.0 0.0 0.0 0.39948830526833434 0.0 60-61 0.0 0.0 0.0 0.4662464684385275 0.0 62-63 0.0 0.0 0.0 0.5353509705164838 0.0 64-65 0.0 0.0 0.0 0.6037162151303503 0.0 66-67 0.0 0.0 0.0 0.6843917036027551 0.0 68-69 0.0 0.0 0.0 0.779627349954841 0.0 70-71 0.0 0.0 0.0 0.8837662275048774 0.0 72-73 0.0 0.0 0.0 0.98935147835422 0.0 74-75 0.0 0.0 0.0 1.0984723083796442 0.0 76-77 0.0 0.0 0.0 1.220160515294594 0.0 78-79 0.0 0.0 0.0 1.2540377921272294 0.0 80-81 0.0 0.0 0.0 1.2548091912201926 0.0 82-83 0.0 0.0 0.0 1.2548091912201926 0.0 84-85 0.0 0.0 0.0 1.2548091912201926 0.0 86-87 0.0 0.0 0.0 1.2548091912201926 0.0 88-89 0.0 0.0 0.0 1.2548091912201926 0.0 90-91 0.0 0.0 0.0 1.2548091912201926 0.0 92-93 0.0 0.0 0.0 1.2548091912201926 0.0 94-95 0.0 0.0 0.0 1.2548091912201926 0.0 96-97 0.0 0.0 0.0 1.2548091912201926 0.0 98-99 0.0 0.0 0.0 1.2548091912201926 0.0 100-101 0.0 0.0 0.0 1.2548091912201926 0.0 102-103 0.0 0.0 0.0 1.2548091912201926 0.0 104-105 0.0 0.0 0.0 1.2548091912201926 0.0 106-107 0.0 0.0 0.0 1.2548091912201926 0.0 108-109 0.0 0.0 0.0 1.2548091912201926 0.0 110-111 0.0 0.0 0.0 1.2548091912201926 0.0 112-113 0.0 0.0 0.0 1.2548091912201926 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 380 0.0 1929.6838 108-109 CCGCTCG 125 0.0 1618.2728 108-109 CCGCGCC 300 0.0 1517.1307 108-109 CGTATCG 20 0.0036608935 1493.8862 110-111 CCGTTCG 55 1.508306E-8 1379.2098 108-109 CTCCGCC 360 0.0 1327.8988 110-111 CCGCGCA 50 2.0962683E-5 1011.4205 108-109 CGATTGT 30 0.008235182 995.9242 110-111 CGTGTCG 60 2.1933276E-5 995.9242 110-111 CCGCGCG 250 0.0 910.27844 108-109 TTGTTCG 200 0.0 884.9929 108-109 CAGCGCG 120 3.2196112E-10 842.8504 108-109 CCGCACC 250 0.0 809.1364 108-109 CGTCGCC 245 0.0 722.4432 108-109 CCTCGCG 200 5.456968E-12 632.1378 108-109 CCGTACG 40 5.865215E-6 632.1378 108-109 CATCGCC 240 0.0 632.1378 108-109 GGTCGCC 210 7.2759576E-12 602.036 108-109 CGCCGTC 100 1.01441736E-4 597.55444 110-111 CCTCTCG 300 0.0 589.99524 108-109 >>END_MODULE