FastQCFastQC Report
Fri 27 May 2016
SRR939904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555615
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37890.24356926360314088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27890.1792860058562048No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA20570.13223066118544757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18690.12014540872902356No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC15970.10266036262185695No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62200.039.749891
GGTATCA43450.037.7069241
TCGACCG350.002790956533.99691436-37
ATCAACG74900.032.683343
TCAACGC75700.032.49534
CAACGCA76150.032.3032725
AACGCAG78400.031.4167926
TATCAAC78850.031.1215972
GTACATG52750.030.9461291
TAGGACC17650.030.0338974
TACATGG55100.028.7538782
GTCCTAC43500.028.4873641
ACGCAGA88100.028.0252677
CGCAGAG88700.027.7686188
GTGTAGC11150.027.2504391
ACATGGG55600.026.1385443
GTAGGAC46400.025.9298363
TCCTACA51250.025.4516932
AGAGTAC78400.025.23208410-11
GCAGAGT99250.024.7569479
Produced by FastQC (version 0.11.5)