##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939903_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 279433 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.608203039726874 33.0 31.0 34.0 25.0 34.0 2 30.56178046257958 34.0 31.0 34.0 25.0 34.0 3 30.82401148039065 34.0 31.0 34.0 26.0 34.0 4 33.602126448916195 37.0 35.0 37.0 24.0 37.0 5 34.15137081160779 37.0 35.0 37.0 30.0 37.0 6 33.65757802407017 37.0 35.0 37.0 25.0 37.0 7 33.73505276756861 37.0 35.0 37.0 27.0 37.0 8 33.47054929088547 37.0 35.0 37.0 23.0 37.0 9 34.81471766040518 39.0 37.0 39.0 17.0 39.0 10-11 35.084488947261065 39.0 37.0 39.0 23.5 39.0 12-13 35.01188657030487 39.0 37.0 39.0 22.0 39.0 14-15 36.39010424681408 41.0 38.0 41.0 22.5 41.0 16-17 36.546263326092486 41.0 38.0 41.0 26.0 41.0 18-19 36.5601986880576 41.0 38.0 41.0 26.5 41.0 20-21 36.60886330533616 41.0 38.0 41.0 27.0 41.0 22-23 36.68216173465554 41.0 38.0 41.0 27.5 41.0 24-25 36.781459240676654 41.0 38.0 41.0 28.5 41.0 26-27 36.724790915890395 41.0 38.0 41.0 29.5 41.0 28-29 36.598372418433044 40.0 38.0 41.0 29.5 41.0 30-31 36.44687277451124 40.0 38.0 41.0 29.5 41.0 32-33 36.23302007994761 40.0 37.0 41.0 30.0 41.0 34-35 36.06155500603007 40.0 37.0 41.0 28.5 41.0 36-37 36.09621805584881 40.0 37.5 41.0 28.0 41.0 38-39 35.92694313126939 40.0 37.0 41.0 27.0 41.0 40-41 35.723674369169 40.0 37.0 41.0 25.5 41.0 42-43 35.42380642229085 40.0 37.0 41.0 22.5 41.0 44-45 35.19504854473165 40.0 37.0 41.0 12.5 41.0 46-47 34.98019918907215 40.0 36.0 41.0 2.0 41.0 48-49 34.79344422455472 40.0 36.0 41.0 2.0 41.0 50-51 34.618479206106656 40.0 36.0 41.0 2.0 41.0 52-53 34.49458009612322 40.0 35.5 41.0 2.0 41.0 54-55 34.29935977497289 40.0 35.0 41.0 2.0 41.0 56-57 34.06261787262063 40.0 35.0 41.0 2.0 41.0 58-59 33.87954536507857 40.0 35.0 41.0 2.0 41.0 60-61 33.613841242802394 39.0 35.0 41.0 2.0 41.0 62-63 33.35137224307795 39.0 34.0 41.0 2.0 41.0 64-65 33.07029413133023 39.0 34.0 41.0 2.0 41.0 66-67 32.750541274652605 38.0 34.0 40.5 2.0 41.0 68-69 32.359986114739485 37.0 33.0 40.0 2.0 41.0 70-71 31.91776025022098 37.0 33.0 39.5 2.0 41.0 72-73 31.504090425969732 36.0 32.0 39.0 2.0 41.0 74-75 31.1112789112238 36.0 32.0 39.0 2.0 41.0 76-77 30.674147291121663 35.0 32.0 38.0 2.0 40.0 78-79 30.23042911896591 35.0 31.0 37.0 2.0 39.0 80-81 29.822764312017547 35.0 31.0 37.0 2.0 39.0 82-83 29.60692187393758 35.0 31.0 36.0 2.0 39.0 84-85 29.180515543976554 35.0 31.0 36.0 2.0 37.0 86-87 28.540927521087344 35.0 31.0 36.0 2.0 37.0 88-89 14.744777817938468 18.0 2.0 18.5 2.0 19.0 90-91 2.802512587990681 2.0 2.0 2.0 2.0 2.0 92-93 2.2917819298364903 2.0 2.0 2.0 2.0 2.0 94-95 2.092175941996829 2.0 2.0 2.0 2.0 2.0 96-97 2.0001109389370617 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3028543733849851 1101 2 0.36001936935218026 1101 3 0.5099069890979706 1101 4 0.21353976634365068 1101 5 0.4728722801104652 1101 6 0.0851228231783594 1101 7 0.1345219668225468 1101 8 0.47975140292395224 1101 9 0.5866191864267947 1101 10-11 0.42425956116449015 1101 12-13 0.26670714011375907 1101 14-15 0.30974009469568387 1101 16-17 0.3521677760032702 1101 18-19 0.3735680629844609 1101 20-21 0.44679128821319836 1101 22-23 0.3941715668742276 1101 24-25 0.28211934081089396 1101 26-27 0.34057329560091176 1101 28-29 0.5415734054631685 1101 30-31 0.6537068156996853 1101 32-33 0.6211518122267847 1101 34-35 0.6063704991710779 1101 36-37 0.6522079333296915 1101 38-39 0.7021633929873374 1101 40-41 0.7165781572473193 1101 42-43 0.4017690578819142 1101 44-45 0.22112777586518462 1101 46-47 0.33767525355261085 1101 48-49 0.47473331046788303 1101 50-51 0.22922061699219398 1101 52-53 -0.002226532207423304 1101 54-55 0.19327133007020336 1101 56-57 0.20190641630936312 1101 58-59 0.3694547346204189 1101 60-61 0.24160608875641287 1101 62-63 0.2927880934660223 1101 64-65 0.17089192551200938 1101 66-67 0.08369461512513965 1101 68-69 0.15872636876191137 1101 70-71 0.21048529102286295 1101 72-73 -0.03911410282989536 1101 74-75 -0.07610627885314258 1101 76-77 -0.15794558121104174 1101 78-79 -0.04126553011670708 1101 80-81 -0.08263870373093951 1101 82-83 -0.16202869560778765 1101 84-85 0.009341335070988777 1101 86-87 -0.07068254329109536 1101 88-89 0.5039547925132908 1101 90-91 -0.13999411208896184 1101 92-93 -0.16472127158507455 1101 94-95 -0.01757609177381836 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.17096761614380895 1102 2 -0.08238900761117307 1102 3 0.059645208993256205 1102 4 -0.42520368915373297 1102 5 0.059259714665437 1102 6 0.22386627867573594 1102 7 -0.43877646249682556 1102 8 -0.3710339373901874 1102 9 -0.17254311723812776 1102 10-11 -0.22233729747425457 1102 12-13 -0.2856488808286457 1102 14-15 -0.20334891053991555 1102 16-17 -0.08369609834227987 1102 18-19 -0.03873560193700598 1102 20-21 0.0580478327158076 1102 22-23 0.018517116612443374 1102 24-25 0.011176932433933473 1102 26-27 0.16648952596740685 1102 28-29 -0.14690826992950434 1102 30-31 0.018890061772978584 1102 32-33 0.033455477148244483 1102 34-35 -0.020592851614267715 1102 36-37 -0.02580253787449749 1102 38-39 -0.35281042900219717 1102 40-41 -0.5033171307107907 1102 42-43 -0.36786444997149204 1102 44-45 -0.5668303393180594 1102 46-47 -0.286670296185612 1102 48-49 -0.4284080507886614 1102 50-51 -0.4082139379816283 1102 52-53 -0.4472527102178958 1102 54-55 -0.25175484794027625 1102 56-57 -0.4381459397115819 1102 58-59 -0.3700740611911044 1102 60-61 -0.3316923405629524 1102 62-63 -0.41401818925649536 1102 64-65 -0.30031226296966196 1102 66-67 -0.26185512309475456 1102 68-69 -0.16980766265169933 1102 70-71 0.014150212488829084 1102 72-73 -0.05874761068329448 1102 74-75 -0.10490209037146414 1102 76-77 -0.16710788487596417 1102 78-79 -0.10801945681827974 1102 80-81 0.06264925438424385 1102 82-83 -0.22354702021511486 1102 84-85 -0.6883026439866029 1102 86-87 -0.510473119207326 1102 88-89 1.3979338501049128 1102 90-91 0.3495346837225566 1102 92-93 -0.08226053860078153 1102 94-95 0.06095793963979412 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.6287568258512302 1103 2 0.8597713671477152 1103 3 0.6540789372930647 1103 4 0.6995827533924199 1103 5 0.7578957025408855 1103 6 0.4629817374798151 1103 7 0.5339846287128722 1103 8 0.24588532411436148 1103 9 0.43916809797267575 1103 10-11 0.4405725950581214 1103 12-13 0.3868573192264648 1103 14-15 0.5363521090247048 1103 16-17 0.5573827112471434 1103 18-19 0.5080059246543414 1103 20-21 0.6370985353887235 1103 22-23 0.6187237851161669 1103 24-25 0.6894147389922125 1103 26-27 0.6464495700566815 1103 28-29 0.6255152627241216 1103 30-31 0.41110554813285916 1103 32-33 0.5903857609729926 1103 34-35 0.6692941696037025 1103 36-37 0.6760230339083648 1103 38-39 0.4686899843348087 1103 40-41 0.2952293008130411 1103 42-43 0.4865995897088595 1103 44-45 0.31049123687373026 1103 46-47 0.3699293180331864 1103 48-49 0.2989273033033726 1103 50-51 0.3629075830985613 1103 52-53 0.6560539858416234 1103 54-55 0.3266689602713555 1103 56-57 0.15763802281253447 1103 58-59 0.19874655054766777 1103 60-61 0.26617897384872435 1103 62-63 0.3110851449953742 1103 64-65 0.48577894672997957 1103 66-67 0.3648464475858759 1103 68-69 0.4249911800046746 1103 70-71 0.26952359358554645 1103 72-73 0.4926301793415 1103 74-75 0.5665501003146751 1103 76-77 0.4069896626577929 1103 78-79 0.31403207252865073 1103 80-81 0.4006211474677386 1103 82-83 0.4631297502450664 1103 84-85 0.356840646175975 1103 86-87 0.28511106376318907 1103 88-89 0.3556081405430511 1103 90-91 -0.5325678238552882 1103 92-93 -0.29561132394109535 1103 94-95 -0.10134572528167185 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.09782919911338084 1104 2 -0.32880153209305263 1104 3 -0.17371671320236715 1104 4 0.19422308282235434 1104 5 -0.2916024315473891 1104 6 -0.14327871806001014 1104 7 -0.4161955282810794 1104 8 -0.2654002247210485 1104 9 0.08521107828408958 1104 10-11 -0.09697726982413712 1104 12-13 0.05675501579060693 1104 14-15 0.16849404699921422 1104 16-17 0.28798044641635556 1104 18-19 0.32181688849425427 1104 20-21 0.18083486275971694 1104 22-23 0.2353625703432911 1104 24-25 0.34892767169070993 1104 26-27 0.17884887516542136 1104 28-29 0.26438091708006795 1104 30-31 0.200881638726095 1104 32-33 -0.07425199478559108 1104 34-35 0.12825698005281083 1104 36-37 0.13039505810087348 1104 38-39 0.05722106141627137 1104 40-41 0.23618606313448964 1104 42-43 0.08164527237518371 1104 44-45 0.29830490306855495 1104 46-47 0.4043907773048332 1104 48-49 0.13520963504960548 1104 50-51 -0.14929805324167944 1104 52-53 0.0076046228252835135 1104 54-55 0.07449420798722173 1104 56-57 0.14407477483929654 1104 58-59 0.1346943050194227 1104 60-61 0.1161564542244804 1104 62-63 -0.11580328635194803 1104 64-65 -0.2982289029676153 1104 66-67 -0.25813643749970083 1104 68-69 -0.15897163044443374 1104 70-71 -0.1987237362304377 1104 72-73 -0.27957417016306607 1104 74-75 -0.2969552400533608 1104 76-77 -0.21996954465143403 1104 78-79 -0.21590294226995965 1104 80-81 -0.1428417273231517 1104 82-83 -0.20191335874991623 1104 84-85 -0.22763046436320522 1104 86-87 -0.27782485182039096 1104 88-89 0.40620008495174886 1104 90-91 -0.39512090582672066 1104 92-93 -0.29561132394109535 1104 94-95 -0.10134572528167185 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.04210269051288407 1105 2 0.006629584118712728 1105 3 0.0942776483564387 1105 4 0.22761712503582032 1105 5 0.18831997751403406 1105 6 0.15417400386005653 1105 7 0.30549375868064743 1105 8 0.36073494817451035 1105 9 0.6099790539340049 1105 10-11 0.1806504683126846 1105 12-13 0.311897598496067 1105 14-15 0.35549818850913084 1105 16-17 0.520768855178396 1105 18-19 0.192619243666158 1105 20-21 0.2654551411889372 1105 22-23 0.39123989682400406 1105 24-25 0.3704834822789991 1105 26-27 0.32739747553466003 1105 28-29 0.2954266483309951 1105 30-31 0.2504986738041808 1105 32-33 0.12486481575280095 1105 34-35 0.10367923942109769 1105 36-37 0.37223678256572157 1105 38-39 0.6718049145174163 1105 40-41 0.6886772062910254 1105 42-43 0.6640700515778164 1105 44-45 0.5175803883237791 1105 46-47 0.4479234104069789 1105 48-49 0.5577216104999394 1105 50-51 0.33635011800726033 1105 52-53 0.33518932418748904 1105 54-55 0.306905265319692 1105 56-57 0.16519709907461788 1105 58-59 0.250438279870977 1105 60-61 0.4260742477477635 1105 62-63 0.5000217722778615 1105 64-65 0.5528170777718628 1105 66-67 0.45821379452464583 1105 68-69 0.3413714163097197 1105 70-71 0.22407515105063425 1105 72-73 0.6246253458291484 1105 74-75 0.4084673935477561 1105 76-77 0.5132866551389945 1105 78-79 0.4674960897144729 1105 80-81 0.4028365072958806 1105 82-83 0.5502135311258414 1105 84-85 0.5265640618580356 1105 86-87 0.35149130006823626 1105 88-89 1.4334411158384341 1105 90-91 -0.8009888764868673 1105 92-93 -0.29561132394109535 1105 94-95 -0.10134572528167185 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 0.7012936180687426 1106 2 0.892921778721675 1106 3 0.7949619505133185 1106 4 0.8274575351335045 1106 5 0.7331139614583222 1106 6 1.2303177314932867 1106 7 1.0304550702194817 1106 8 1.1868182250871726 1106 9 1.2302880780175727 1106 10-11 1.156536675876282 1106 12-13 1.2249029665200553 1106 14-15 1.209618509623354 1106 16-17 1.0591973782463953 1106 18-19 1.1135497011572113 1106 20-21 0.9673242774690394 1106 22-23 0.9745107644127486 1106 24-25 0.6779842076876506 1106 26-27 0.6411427603784574 1106 28-29 1.089066023512352 1106 30-31 1.024014004106924 1106 32-33 1.0982181381244018 1106 34-35 1.1200674297683264 1106 36-37 1.3465331361782518 1106 38-39 1.2990778616109466 1106 40-41 1.4079319698077981 1106 42-43 1.4877594799558054 1106 44-45 1.5264303738156144 1106 46-47 1.1158011065091173 1106 48-49 1.4015713541888886 1106 50-51 1.3251425544131052 1106 52-53 1.3486456545869459 1106 54-55 1.4946410231299225 1106 56-57 1.2574509437404444 1106 58-59 1.2530618411438326 1106 60-61 1.615411949653165 1106 62-63 1.4857795825109577 1106 64-65 1.6433057613969737 1106 66-67 1.411291945216206 1106 68-69 1.468886166285543 1106 70-71 1.6353878988985855 1106 72-73 1.4057756021401993 1106 74-75 1.2954849967975868 1106 76-77 1.334926804089573 1106 78-79 1.4510051331422709 1106 80-81 1.4036767723118402 1106 82-83 1.2735064027768708 1106 84-85 1.3339982914740567 1106 86-87 1.2479633960156207 1106 88-89 1.2502452071432018 1106 90-91 -0.10786091440155943 1106 92-93 -0.29561132394109535 1106 94-95 -0.10134572528167185 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.31623064282044666 1107 2 0.09761597867653649 1107 3 -0.2994298346367614 1107 4 -0.08938967768526851 1107 5 -0.2268160769077383 1107 6 -0.036642322670559224 1107 7 -0.04433617329213746 1107 8 0.030973043412608092 1107 9 -0.12386448348095769 1107 10-11 -0.34783592624513915 1107 12-13 -0.4106364151093729 1107 14-15 -0.22987596115073927 1107 16-17 -0.46009849176037676 1107 18-19 -0.4160542257216022 1107 20-21 -0.34134757989949804 1107 22-23 -0.4600356133800787 1107 24-25 -0.46233964697270125 1107 26-27 -0.47115694623404636 1107 28-29 -0.22047471221321757 1107 30-31 -0.27824282279446066 1107 32-33 -0.09877464002951086 1107 34-35 -0.23603164493263762 1107 36-37 -0.2571849861417661 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-0.21643054312991694 2216 60-61 -0.16466346243065288 2216 62-63 -0.2900458270889317 2216 64-65 -0.4196966132391253 2216 66-67 -0.613979548221252 2216 68-69 -0.702028018936069 2216 70-71 -0.7709431392843626 2216 72-73 -0.5998778202171415 2216 74-75 -0.6755772305912089 2216 76-77 -0.8357886668136842 2216 78-79 -0.8963425274934167 2216 80-81 -0.7175240583140123 2216 82-83 -0.7497853992639278 2216 84-85 -0.8892175023399673 2216 86-87 -1.1012372756387734 2216 88-89 -1.1623825910027943 2216 90-91 -0.8009888764868673 2216 92-93 -0.29561132394109535 2216 94-95 -0.10134572528167185 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6427.0 3 1306.0 4 1879.0 5 2396.0 6 4265.0 7 4020.0 8 3880.0 9 2923.0 10 2325.0 11 1866.0 12 1485.0 13 1450.0 14 1371.0 15 1422.0 16 1493.0 17 1586.0 18 1854.0 19 2287.0 20 2319.0 21 3130.0 22 3726.0 23 4752.0 24 7573.0 25 14842.0 26 26769.0 27 72845.0 28 95193.0 29 3887.0 30 162.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32129705510802 21.793059516950397 13.88919705260295 20.996446375338632 2 13.055552573283375 25.063611865625973 39.06394826592802 22.816887295162637 3 18.710393469621568 32.95370735025599 26.9399591851348 21.395939994987646 4 11.315512463390357 20.89954099205866 37.344341885727786 30.440604658823194 5 11.34304097042954 44.219635587667696 31.947840844399728 12.489482597503034 6 26.667215396892995 39.401216033897214 19.061098724917958 14.870469844291833 7 25.241113254340036 36.819201740667715 22.15736867155991 15.782316333432345 8 23.70192496949179 38.21345367225775 20.276059019514516 17.80856233873594 9 25.544584927334995 21.891473090150413 20.121102375166856 32.442839607347736 10-11 21.644762151861364 32.211893748937584 26.097894840435526 20.045449258765533 12-13 24.1893606322996 30.2044022625074 25.861726512886307 19.74451059230669 14-15 21.856402071337317 31.576800163187595 24.42356486170209 22.143232903772997 16-17 19.822104046408263 33.84245955202142 26.389510186699493 19.945926214870827 18-19 19.8031636962279 33.32635459165414 27.261291246152748 19.609190465965217 20-21 22.358654651387507 31.31553707295775 26.070781828202506 20.25502644745224 22-23 21.431485524102637 32.255305443223705 25.739720144580037 20.573488888093618 24-25 21.640429011605644 32.55002093525103 25.6521241227772 20.157425930366134 26-27 20.86600365740625 31.899417749514196 26.827540054324295 20.407038538755266 28-29 20.231472451807885 32.771352957912306 26.738682704162247 20.258491886117557 30-31 21.109711451403378 32.89160550113981 26.2098606821671 19.78882236528971 32-33 20.26650777120485 33.17479574417831 26.912918845216495 19.64577763940035 34-35 20.820554479964787 33.237305543726045 26.349786889880576 19.59235308642859 36-37 20.36803097701417 33.289196336867874 26.651111357642083 19.691661328475877 38-39 20.493463549401824 34.26867979086221 26.07762862654017 19.160228033195793 40-41 19.7805914118948 34.33810609341059 26.640733199013717 19.240569295680896 42-43 19.667684203368964 34.612232628215 25.707593591308115 20.012489577107928 44-45 19.723511539438793 34.33166447771022 25.744453947815753 20.20037003503523 46-47 19.9448526122541 34.58557149656626 26.246721038674746 19.222854852504895 48-49 19.405794611927053 34.52145781442354 26.477103552921644 19.59564402072776 50-51 18.03616609348216 35.042210476214336 27.08609935118615 19.835524079117356 52-53 18.7009050470059 35.4126033789853 25.643893169382288 20.24259840462651 54-55 18.557208321926204 34.66300207028223 25.895705730047364 20.884083877744196 56-57 19.97562922060029 33.88719299438506 25.07166297466656 21.0655148103481 58-59 20.175677174850502 33.50123285367154 25.56355190689718 20.759538064580777 60-61 19.73048995644752 33.59982536064101 26.55430818836716 20.11537649454431 62-63 19.019407156635044 33.70772242362212 26.437106569374414 20.835763850368423 64-65 18.977357720813217 34.040539234807625 25.99335082112707 20.988752223252085 66-67 20.389503029348717 33.03278424523947 24.84298561730361 21.734727108108203 68-69 20.451666237224085 32.276546709038335 25.64452174410948 21.6272653096281 70-71 21.131541371276835 32.14294660974187 25.24236579072622 21.483146228255073 72-73 21.314232749889957 31.30929417785301 26.179978742668187 21.196494329588848 74-75 20.743791892868774 32.66507534901032 25.687731942898655 20.903400815222252 76-77 20.303793753779974 31.45924067665594 26.771712718254463 21.465252851309614 78-79 21.261165507171693 31.299028028285953 26.898851956826707 20.540954507715654 80-81 21.677647235652195 31.08544803226534 26.53480440749661 20.702100324585857 82-83 21.145790649944622 30.612511474189557 27.496184216883208 20.745513658982613 84-85 21.46811579162089 30.883252872781668 26.83093979594393 20.817691539653513 86-87 20.74852511993186 31.042355943091195 26.9801506283338 21.228968308643147 88-89 32.14912125292325 19.421461980015593 32.81593260576855 15.61348416129261 90-91 34.17600549684512 15.913269848643207 17.59498509658189 32.31573955792978 92-93 24.588678568224633 18.282348015908614 36.3589605573996 20.77001285846715 94-95 20.13815090329437 8.376856483761436 32.008955185194786 39.47603742774941 96-97 34.04184448839388 8.671801811179348 18.518441414246556 38.76791228618022 98-99 57.48749478949562 14.331492288453523 19.25020842017507 8.930804501875782 100-101 34.60944976520249 7.879255473044652 13.779609338486761 43.7316854232661 102-103 27.691929776078727 2.7366449423884425 38.3168674051386 31.25455787639423 104-105 45.800383332150204 4.93788599417903 40.33861006601832 8.923120607652447 106-107 10.211901080413494 4.474331670410674 11.729925800585427 73.5838414485904 108-109 23.207432014516677 12.214653488057738 23.631176448902853 40.94673804852273 110-111 44.231905249305456 3.055417458692791 27.799678315543208 24.912998976458546 112-113 31.079764539886 3.2806895084378187 41.70869089701046 23.930855054665717 114-115 34.804368827823104 4.959209961356806 26.779197080291972 33.457224130528125 116-117 25.519833610470993 5.945003769428597 24.618378771433612 43.916783848666796 118-119 26.680956390823958 5.673786011380744 20.46301258006073 47.18224501773457 120-121 30.76908999405225 5.283433225053759 33.14544539506794 30.80203138582605 122-123 38.26383464519618 2.0999819744036534 37.37907829117347 22.2571050892267 124-125 43.4482364006337 2.730423027819202 21.896311872601714 31.92502869894538 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 144.0 1 332.5 2 652.0 3 931.5 4 1142.0 5 1138.5 6 976.0 7 813.5 8 681.5 9 562.0 10 478.0 11 412.0 12 344.5 13 278.5 14 241.0 15 231.0 16 217.0 17 239.0 18 282.0 19 345.0 20 447.5 21 540.0 22 659.5 23 855.5 24 1156.5 25 1563.0 26 2084.5 27 2667.5 28 3252.0 29 3962.0 30 4789.5 31 5834.5 32 7070.5 33 8443.5 34 9899.5 35 11252.0 36 11974.0 37 11910.5 38 11721.5 39 11508.0 40 11081.5 41 10563.0 42 10289.5 43 10707.0 44 11408.0 45 11571.5 46 11303.0 47 10727.5 48 9838.0 49 9144.5 50 8757.0 51 8124.0 52 7001.5 53 5747.5 54 4642.0 55 3677.0 56 2928.5 57 2365.5 58 1895.5 59 1494.0 60 1158.5 61 856.5 62 622.0 63 447.5 64 300.0 65 194.0 66 127.5 67 101.5 68 82.0 69 62.0 70 48.5 71 38.5 72 28.0 73 23.0 74 21.5 75 16.0 76 12.0 77 8.5 78 6.5 79 6.5 80 6.0 81 3.5 82 2.5 83 4.0 84 4.0 85 2.0 86 1.0 87 1.0 88 1.5 89 3.0 90 3.5 91 2.0 92 1.5 93 3.0 94 2.5 95 1.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0014314701556365928 3 0.04401770728582522 4 0.048312117752735 5 0.04795425021382586 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.789337694545741E-4 12-13 0.0030418740807277595 14-15 0.0 16-17 0.0 18-19 0.004652278005818926 20-21 0.00393654292800063 22-23 0.0010736026167274444 24-25 0.0 26-27 0.0 28-29 0.0016104039250911667 30-31 0.0 32-33 0.0014314701556365928 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 3.578675389091482E-4 50-51 0.0 52-53 0.0 54-55 8.946688472728704E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.578675389091482E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.578675389091482E-4 80-81 0.0 82-83 5.368013083637222E-4 84-85 0.0 86-87 0.0012525363861820187 88-89 19.20209853524816 90-91 26.0416629388798 92-93 40.16275815669588 94-95 44.77244992538461 96-97 48.429498305497205 98-99 93.13180619325563 100-101 51.76052935766356 102-103 53.38095357384418 104-105 74.79359989693415 106-107 81.60006155321669 108-109 12.632724123492931 110-111 38.81359753500839 112-113 52.06346422935015 114-115 86.6644240300895 116-117 66.53383816514156 118-119 71.9510580353788 120-121 80.44522300515688 122-123 76.1760421997402 124-125 49.96528684872582 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 279433.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.60760133736151 #Duplication Level Percentage of deduplicated Percentage of total 1 70.19564365209966 25.696941384355547 2 10.860056204222557 7.9512121605103765 3 5.065929963835638 5.563546335572676 4 3.1259234261365796 4.577302343805084 5 2.181472833036591 3.992924390004405 6 1.5189323670396282 3.336268233060094 7 1.1811695121087964 3.026784782778723 8 0.8893746593254015 2.60462983745088 9 0.7424779449028087 2.446230294790693 >10 3.997189474109073 25.608779054336622 >50 0.14989460527888032 3.748545211267582 >100 0.07694589737649189 5.7038466804024734 >500 0.00899367631673282 2.0237095841448487 >1k 0.005995784211155213 3.719279707520007 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2553 0.9136358268350553 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2127 0.7611842552597581 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1691 0.6051540082953696 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1441 0.5156871235680826 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1344 0.4809739722938951 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1011 0.3618040818371488 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 764 0.2734107997265892 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 738 0.26410624371495134 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 669 0.23941338353022013 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 630 0.22545654951276334 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 609 0.21794133119567125 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 569 0.2036266296393053 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 541 0.19360633854984916 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 507 0.18143884222693812 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 505 0.18072310714911982 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 499 0.17857590191566494 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 489 0.17499722652657346 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 484 0.1732078888320277 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 483 0.17285002129311858 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 460 0.16461906789820815 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 451 0.16139826004802582 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 403 0.1442206181803867 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 398 0.14243128048584097 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 370 0.13241098939638482 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 351 0.125611506157111 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 329 0.11773842030110973 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 312 0.11165467213965423 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 302 0.10807599675056274 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 300 0.10736026167274446 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 285 0.10199224858910723 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 284 0.10163438105019806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.5786753890914815E-4 0.0 16-17 0.0 0.0 0.0 5.368013083637222E-4 0.0 18-19 0.0 0.0 0.0 0.0016104039250911667 0.0 20-21 0.0 0.0 0.0 0.0026840065418186113 0.0 22-23 0.0 0.0 0.0 0.007157350778182964 0.0 24-25 0.0 0.0 0.0 0.01180962878400189 0.0 26-27 0.0 0.0 0.0 0.021472052334548887 0.0 28-29 0.0 0.0 0.0 0.03614462142982396 0.0 30-31 0.0 0.0 0.0 0.08177273264074036 0.0 32-33 0.0 0.0 0.0 0.13777900248002206 0.0 34-35 0.0 0.0 0.0 0.20147942440585043 0.0 36-37 0.0 0.0 0.0 0.3002508651447753 0.0 38-39 0.0 0.0 0.0 0.46504886681243807 0.0 40-41 0.0 0.0 0.0 0.6647389535237427 0.0 42-43 0.0 0.0 0.0 0.8288212201135872 0.0 44-45 0.0 0.0 0.0 1.0018501751761604 0.0 46-47 0.0 0.0 0.0 1.1915199707980089 0.0 48-49 0.0 0.0 0.0 1.409819169532589 0.0 50-51 0.0 0.0 0.0 1.5948366871486188 0.0 52-53 0.0 0.0 0.0 1.7546245432715535 0.0 54-55 0.0 0.0 0.0 1.9174542734752158 0.0 56-57 0.0 0.0 0.0 2.1065872677887008 0.0 58-59 0.0 0.0 0.0 2.289278646401821 0.0 60-61 0.0 0.0 0.0 2.477874839406942 0.0 62-63 0.0 0.0 0.0 2.6514405957778786 0.0 64-65 0.0 0.0 0.0 2.8573933644200933 0.0 66-67 0.0 0.0 0.0 3.079092304774311 0.0 68-69 0.0 0.0 0.0 3.3333571911692603 0.0 70-71 0.0 0.0 0.0 3.61732508329367 0.0 72-73 0.0 0.0 0.0 3.883220664703167 0.0 74-75 0.0 0.0 0.0 4.15484212673521 0.0 76-77 0.0 0.0 0.0 4.445609502098893 0.0 78-79 0.0 0.0 0.0 4.524698228197815 0.0 80-81 0.0 0.0 0.0 4.527024367200724 0.0 82-83 0.0 0.0 0.0 4.527024367200724 0.0 84-85 0.0 0.0 0.0 4.527024367200724 0.0 86-87 0.0 0.0 0.0 4.527024367200724 0.0 88-89 0.0 0.0 0.0 4.527024367200724 0.0 90-91 0.0 0.0 0.0 4.527024367200724 0.0 92-93 0.0 0.0 0.0 4.527024367200724 0.0 94-95 0.0 0.0 0.0 4.527024367200724 0.0 96-97 0.0 0.0 0.0 4.527024367200724 0.0 98-99 0.0 0.0 0.0 4.527024367200724 0.0 100-101 0.0 0.0 0.0 4.527024367200724 0.0 102-103 0.0 0.0 0.0 4.527024367200724 0.0 104-105 0.0 0.0 0.0 4.527024367200724 0.0 106-107 0.0 0.0 0.0 4.527024367200724 0.0 108-109 0.0 0.0 0.0 4.527024367200724 0.0 110-111 0.0 0.0 0.0 4.527024367200724 0.0 112-113 0.0 0.0 0.0 4.527024367200724 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTGTC 5 1.9830852E-4 6383.92 110-111 CAGCGTG 5 3.5372723E-4 4787.94 108-109 TTGCTCG 5 3.5372723E-4 4787.94 108-109 GCCGGTG 5 3.5372723E-4 4787.94 108-109 CCGTACG 5 3.5372723E-4 4787.94 108-109 CGTGTCG 5 3.5372723E-4 4787.94 108-109 CCGTTGT 5 3.5372723E-4 4787.94 108-109 TGTCGCT 25 1.2592263E-6 2553.5679 110-111 CGCGGCT 10 0.0014147159 2393.97 108-109 CCGCGCC 50 0.0 2393.97 108-109 GATCGAT 15 0.0017844139 2127.9734 110-111 CGTCGAG 15 0.0017844139 2127.9734 110-111 CACCGCT 15 0.0017844139 2127.9734 110-111 CTGCTCG 20 0.0031719692 1595.98 110-111 TCGTTCG 15 0.0031826764 1595.98 108-109 CCGGACG 15 0.0031826764 1595.98 108-109 CGTCTTG 20 0.0031719692 1595.98 110-111 CCGTATG 15 0.0031826764 1595.98 108-109 GTCGTGT 20 0.0031719692 1595.98 110-111 CGGTGTT 20 0.0031719692 1595.98 110-111 >>END_MODULE