##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939902_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 162459 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.08015560849199 33.0 31.0 34.0 27.0 34.0 2 31.17051071347232 34.0 31.0 34.0 27.0 34.0 3 31.289309918194746 34.0 31.0 34.0 27.0 34.0 4 34.271046848743374 37.0 35.0 37.0 30.0 37.0 5 34.52186090028869 37.0 35.0 37.0 31.0 37.0 6 34.485574821955076 37.0 36.0 37.0 31.0 37.0 7 34.55031731082919 37.0 36.0 37.0 31.0 37.0 8 34.42943758117433 37.0 36.0 37.0 31.0 37.0 9 35.947765282317384 39.0 38.0 39.0 30.0 39.0 10-11 36.05719904714421 39.0 38.0 39.0 31.0 39.0 12-13 35.988603278365616 39.0 38.0 39.0 31.0 39.0 14-15 37.4564782498969 41.0 39.0 41.0 31.0 41.0 16-17 37.52097760050228 41.0 39.0 41.0 31.0 41.0 18-19 37.56689072319786 41.0 39.0 41.0 31.0 41.0 20-21 37.636588308434746 41.0 39.0 41.0 31.0 41.0 22-23 37.6843203515964 41.0 39.0 41.0 31.0 41.0 24-25 37.74248271871672 41.0 39.0 41.0 31.0 41.0 26-27 37.64273447454434 41.0 38.5 41.0 31.0 41.0 28-29 37.54891695750928 41.0 38.0 41.0 30.0 41.0 30-31 37.39082783963954 40.0 38.0 41.0 30.0 41.0 32-33 37.14798195236952 40.0 38.0 41.0 30.0 41.0 34-35 37.02088219181455 40.0 38.0 41.0 30.0 41.0 36-37 37.117617368074406 40.5 38.0 41.0 30.0 41.0 38-39 37.00238213949366 41.0 38.0 41.0 30.0 41.0 40-41 36.87993586074025 41.0 38.0 41.0 30.0 41.0 42-43 36.72172671258595 41.0 38.0 41.0 30.0 41.0 44-45 36.57199047144202 40.0 38.0 41.0 30.0 41.0 46-47 36.39837435906906 40.0 38.0 41.0 30.0 41.0 48-49 36.270265113043905 40.0 38.0 41.0 29.0 41.0 50-51 36.13612665349412 40.0 37.0 41.0 28.5 41.0 52-53 36.046260902750845 40.0 37.0 41.0 28.5 41.0 54-55 35.88185018989407 40.0 37.0 41.0 28.0 41.0 56-57 35.62795536104494 40.0 36.0 41.0 27.5 41.0 58-59 35.465748281104766 40.0 36.0 41.0 26.5 41.0 60-61 35.1536018318468 39.5 35.0 41.0 26.0 41.0 62-63 34.847746200579834 39.0 35.0 41.0 24.0 41.0 64-65 34.55921494038496 39.0 35.0 41.0 22.0 41.0 66-67 34.18763503407014 38.0 35.0 40.5 22.0 41.0 68-69 33.73802620968983 37.0 34.0 40.0 20.0 41.0 70-71 33.22211758043568 37.0 34.0 39.0 15.5 41.0 72-73 32.754978179109806 36.0 33.5 39.0 7.5 41.0 74-75 32.30798847709268 36.0 33.0 39.0 2.0 40.5 76-77 31.779295083682655 35.0 33.0 37.0 2.0 39.5 78-79 31.305784844176067 35.0 32.0 37.0 2.0 39.0 80-81 30.88945210791646 35.0 32.0 36.5 2.0 39.0 82-83 30.67197877618353 35.0 32.0 36.0 2.0 37.5 84-85 30.220661828522886 35.0 32.0 36.0 2.0 37.0 86-87 29.483494297022634 35.0 32.0 35.5 2.0 37.0 88-89 15.109138305664814 18.0 2.0 18.5 2.0 19.0 90-91 2.839885755790692 2.0 2.0 2.0 2.0 2.0 92-93 2.3062895869111593 2.0 2.0 2.0 2.0 2.0 94-95 2.0983232692556273 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.17643901280962737 1101 2 -0.3870367769259886 1101 3 -0.23452116560214264 1101 4 -0.46857038160614906 1101 5 -0.4739002737702833 1101 6 -0.32013057600619277 1101 7 -0.6460112077088098 1101 8 -0.6008344713142435 1101 9 -0.31730851780973524 1101 10-11 -0.41539521520550693 1101 12-13 -0.2935577826175191 1101 14-15 -0.5551278024906523 1101 16-17 -0.3665339627352253 1101 18-19 -0.46662095855759134 1101 20-21 -0.45365015876323156 1101 22-23 -0.4757437136273168 1101 24-25 -0.5139142865822492 1101 26-27 -0.21638216679683353 1101 28-29 -0.07308824945135939 1101 30-31 -0.17544834956308364 1101 32-33 -0.6920615987488148 1101 34-35 -0.6962192535902005 1101 36-37 -0.4056252464631882 1101 38-39 -0.5391428115917236 1101 40-41 -0.5150815876081438 1101 42-43 -0.6785198803243233 1101 44-45 -0.6140145671210533 1101 46-47 -0.44290011643534655 1101 48-49 -0.4615224224785379 1101 50-51 -0.5829702163683734 1101 52-53 -0.571868629142692 1101 54-55 -0.5101621811794175 1101 56-57 -0.48909932123082456 1101 58-59 -0.30274361389416526 1101 60-61 -0.4339661750263417 1101 62-63 -0.19518694220492705 1101 64-65 -0.04736013614337509 1101 66-67 -0.04464337842929211 1101 68-69 -0.14239400057822138 1101 70-71 0.03140003861663132 1101 72-73 0.04004305544064124 1101 74-75 -0.11831783819065578 1101 76-77 0.13635677851493 1101 78-79 -0.080457004146826 1101 80-81 0.0047441498412332805 1101 82-83 0.09161317612444009 1101 84-85 0.2044214982382897 1101 86-87 -0.3234535700180956 1101 88-89 -0.23485995265181714 1101 90-91 -0.31461199463754363 1101 92-93 -0.3252080710392993 1101 94-95 -0.11866895330127747 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.0318979500076928 1102 2 0.07934003466821338 1102 3 0.25079284405969204 1102 4 0.04196101936003771 1102 5 0.22822533009445323 1102 6 0.4209322259261725 1102 7 0.2982400000206624 1102 8 0.3557838861736755 1102 9 0.2259765063448569 1102 10-11 0.5100637219925588 1102 12-13 0.3591958405708908 1102 14-15 0.3340026322919485 1102 16-17 0.22322449137105593 1102 18-19 0.5155529544858837 1102 20-21 0.6288619185314488 1102 22-23 0.5490872042470798 1102 24-25 0.6270518969926258 1102 26-27 0.48955986218867054 1102 28-29 0.5884576442684448 1102 30-31 0.6216047905335387 1102 32-33 0.5882282563236529 1102 34-35 0.4869691522069033 1102 36-37 0.43688683083149726 1102 38-39 0.197862019326152 1102 40-41 0.059942566981227685 1102 42-43 -0.21726384167697432 1102 44-45 -0.30841070238675883 1102 46-47 -0.48386630001022723 1102 48-49 -0.6828750794833738 1102 50-51 -0.8945644192669278 1102 52-53 -0.927955585664435 1102 54-55 -0.6570220845610635 1102 56-57 -1.1834471473177786 1102 58-59 -1.0498450631695277 1102 60-61 -1.0093284938669242 1102 62-63 -1.2832545750551745 1102 64-65 -1.4563456433897457 1102 66-67 -1.2132424122457195 1102 68-69 -1.1603650150709655 1102 70-71 -1.2076820869872336 1102 72-73 -1.4803434179893102 1102 74-75 -1.2855642150022462 1102 76-77 -1.1819524002290294 1102 78-79 -0.8952396128424773 1102 80-81 -1.399651985424466 1102 82-83 -1.2021066306388448 1102 84-85 -1.3747089365443195 1102 86-87 -1.5647096086654386 1102 88-89 0.01600961256557376 1102 90-91 -0.06079556951677079 1102 92-93 -0.03631918215041052 1102 94-95 0.07910882447650014 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.09982990119152291 1103 2 0.15640317710580476 1103 3 0.21092989741260482 1103 4 0.06148133262484379 1103 5 -0.2713145622207733 1103 6 -0.21685534137680662 1103 7 0.008939951073031693 1103 8 0.25618525670720516 1103 9 0.012838963515683588 1103 10-11 0.19666186198276847 1103 12-13 0.22195648667955936 1103 14-15 0.13312157502323174 1103 16-17 0.09125190204164113 1103 18-19 0.1998701351026284 1103 20-21 0.30026671539879857 1103 22-23 0.5689795194697851 1103 24-25 0.43423740849532066 1103 26-27 0.6730547226879366 1103 28-29 0.6269500573864093 1103 30-31 0.43381232846794404 1103 32-33 0.6120755397304052 1103 34-35 0.6717562300336226 1103 36-37 0.7073224647032532 1103 38-39 0.7026882552537117 1103 40-41 0.782840266442804 1103 42-43 0.9852226977258667 1103 44-45 0.7774238546372274 1103 46-47 0.7239261620553634 1103 48-49 0.9746775392146176 1103 50-51 0.6493416012910771 1103 52-53 0.698740449577862 1103 54-55 0.7461105635055105 1103 56-57 0.8785792844002813 1103 58-59 1.1247511189156398 1103 60-61 0.8925804635486401 1103 62-63 0.6590459633687118 1103 64-65 0.6970757956704503 1103 66-67 1.0647801036622582 1103 68-69 1.1429536339745567 1103 70-71 0.9795230035658733 1103 72-73 0.6230731263082916 1103 74-75 0.5888263870535582 1103 76-77 0.4981552845482611 1103 78-79 0.8367427660121471 1103 80-81 1.1047728799695626 1103 82-83 1.1085639518379082 1103 84-85 0.8952470105922465 1103 86-87 1.143200327702651 1103 88-89 1.7548642381162356 1103 90-91 -0.4354700678036041 1103 92-93 -0.3252080710392993 1103 94-95 -0.11866895330127747 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.3272849099823425 1104 2 0.38654481792680784 1104 3 0.4662587135165914 1104 4 0.41670940002769896 1104 5 0.09995427877407081 1104 6 -0.10472796341114332 1104 7 -0.0844007573286163 1104 8 0.08497172872474579 1104 9 0.3218609109288124 1104 10-11 0.08917682612808875 1104 12-13 0.23208074341094687 1104 14-15 0.38377841704531335 1104 16-17 0.18267640965705567 1104 18-19 0.17994757331996425 1104 20-21 0.1090911163391226 1104 22-23 0.006416551531579273 1104 24-25 0.22242808035087336 1104 26-27 0.4485135243710374 1104 28-29 0.5358218694802019 1104 30-31 0.6849630366720518 1104 32-33 0.7096931292683379 1104 34-35 0.6150110057196017 1104 36-37 0.6907483156346856 1104 38-39 0.8611106491218621 1104 40-41 1.0258718146145185 1104 42-43 0.8322229545362774 1104 44-45 0.5055603987592363 1104 46-47 0.8629099829992413 1104 48-49 0.8249076808554392 1104 50-51 0.879127658461023 1104 52-53 0.768187912093417 1104 54-55 0.7173675517119875 1104 56-57 0.6144003863145073 1104 58-59 0.7096467156047623 1104 60-61 0.6420915360284454 1104 62-63 0.5692018275357569 1104 64-65 0.35160652402429804 1104 66-67 0.6136135917403323 1104 68-69 0.6138352838826933 1104 70-71 0.8596024172479488 1104 72-73 0.7686451221203114 1104 74-75 0.6091144098109069 1104 76-77 0.7019738578676282 1104 78-79 0.527809714511779 1104 80-81 0.5039155282775774 1104 82-83 0.4009327315933433 1104 84-85 0.700268641931018 1104 86-87 0.7169246713544908 1104 88-89 1.3446194780364262 1104 90-91 -0.769086551081295 1104 92-93 -0.3252080710392993 1104 94-95 -0.11866895330127747 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.9494309066931912 1105 2 -1.114671264766816 1105 3 -0.9855160071644136 1105 4 -0.5857037640674605 1105 5 -0.5924338036155277 1105 6 -1.0060922561830523 1105 7 -0.17290877587387854 1105 8 -0.7579825921690713 1105 9 -0.6388634183255775 1105 10-11 -0.8555389145422723 1105 12-13 -0.6348437074517079 1105 14-15 -0.824048215165682 1105 16-17 -0.6497321941280063 1105 18-19 -0.6195317912768274 1105 20-21 -0.7853377048207122 1105 22-23 -1.0299994247548057 1105 24-25 -0.7362061067738495 1105 26-27 -0.5524175389412704 1105 28-29 -0.49604329735114305 1105 30-31 -0.44283965391090874 1105 32-33 -0.4678169414164657 1105 34-35 -0.573595819544515 1105 36-37 -0.5487534704867727 1105 38-39 -0.4217330842520042 1105 40-41 -0.3721007475197098 1105 42-43 -0.554956873692042 1105 44-45 -0.5937308530458907 1105 46-47 -0.41213371997256587 1105 48-49 -0.5231104843797922 1105 50-51 -0.6442819333912198 1105 52-53 -0.8488030432915536 1105 54-55 -0.7383309357851644 1105 56-57 -0.5057352829109973 1105 58-59 -0.5997038202316816 1105 60-61 -0.5197804712680565 1105 62-63 -0.3151943114016902 1105 64-65 -0.5472495981920176 1105 66-67 -0.8144665177071104 1105 68-69 -0.6065797043365038 1105 70-71 -1.114565326158612 1105 72-73 -0.9099385215674616 1105 74-75 -0.8642647799740004 1105 76-77 -1.0215098390237571 1105 78-79 -0.4826124941984098 1105 80-81 -1.09446366150733 1105 82-83 -1.0215039941040018 1105 84-85 -0.8639462246799106 1105 86-87 -0.3991352207181684 1105 88-89 0.32304351087065797 1105 90-91 -0.8363511250723263 1105 92-93 -0.3252080710392993 1105 94-95 -0.11866895330127747 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.7641201722299158 1106 2 -0.8489932986651212 1106 3 -0.7694849337180862 1106 4 -0.9508167584177443 1106 5 -0.5820524476833242 1106 6 -0.7346233296293789 1106 7 -0.9081488888682259 1106 8 -0.6028272249374353 1106 9 -1.01096793809959 1106 10-11 -0.7214640557852192 1106 12-13 -0.6153874927624443 1106 14-15 -0.7222473677080501 1106 16-17 -1.522331064184499 1106 18-19 -0.8269470455141175 1106 20-21 -0.8521103036907718 1106 22-23 -1.0973016846418133 1106 24-25 -0.967809214118482 1106 26-27 -1.3483568044779943 1106 28-29 -1.2718201335093369 1106 30-31 -1.1824229872442373 1106 32-33 -1.0259384103430236 1106 34-35 -1.389697514459769 1106 36-37 -1.3686687247240599 1106 38-39 -1.2856102028960734 1106 40-41 -1.3349185441298843 1106 42-43 -1.7475416194547577 1106 44-45 -1.2891051468311971 1106 46-47 -1.293171855565781 1106 48-49 -1.1569028572611515 1106 50-51 -1.159147752600262 1106 52-53 -1.1896222523310982 1106 54-55 -1.1327165290055063 1106 56-57 -1.293447147317778 1106 58-59 -1.0144283965028649 1106 60-61 -1.080161827200257 1106 62-63 -1.046726797277401 1106 64-65 -1.272178976723083 1106 66-67 -1.0097701900234952 1106 68-69 -0.9899483484042975 1106 70-71 -0.9890709758761247 1106 72-73 -1.0238156402115308 1106 74-75 -1.250564215002246 1106 76-77 -1.1751468446734776 1106 78-79 -1.104822946175812 1106 80-81 -1.6224297632022449 1106 82-83 -1.4784955195277334 1106 84-85 -1.6117922698776503 1106 86-87 -1.5981818308876612 1106 88-89 0.04892627923223891 1106 90-91 -0.07176779173899295 1106 92-93 -0.3252080710392993 1106 94-95 -0.11866895330127747 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.09293258788443737 1107 2 -0.06063297477848195 1107 3 0.04618645629540907 1107 4 0.17047204050263787 1107 5 0.10939492073772783 1107 6 0.4227315961465976 1107 7 0.36956703555821946 1107 8 0.1221896441583823 1107 9 0.4437722778248343 1107 10-11 0.3114222465089753 1107 12-13 0.2875899026491595 1107 14-15 0.1433143731826405 1107 16-17 0.3020279101744734 1107 18-19 0.07927765084215821 1107 20-21 -0.1444264305463676 1107 22-23 0.16921316016250643 1107 24-25 0.08066413271012607 1107 26-27 0.1036759215678913 1107 28-29 0.2832574643314274 1107 30-31 0.09967046754658071 1107 32-33 0.2098746800753375 1107 34-35 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-0.8363511250723263 2216 92-93 -0.3252080710392993 2216 94-95 -0.11866895330127747 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3031.0 3 399.0 4 548.0 5 827.0 6 1592.0 7 1594.0 8 1121.0 9 849.0 10 861.0 11 732.0 12 699.0 13 736.0 14 777.0 15 815.0 16 815.0 17 923.0 18 1102.0 19 1296.0 20 1392.0 21 1944.0 22 2134.0 23 2952.0 24 4908.0 25 9424.0 26 18285.0 27 46644.0 28 53859.0 29 2129.0 30 71.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.989560442942526 20.923432989246518 13.974602822866078 21.112403744944878 2 14.28606602281191 24.038680528625683 38.095150160963684 23.580103287598718 3 20.76123502099831 29.133465522125203 28.666699508602534 21.43859994827395 4 12.53517917515996 20.3943762585985 36.39605131078992 30.674393255451616 5 12.168820131301809 41.64459827312255 32.99789375146267 13.188687844112973 6 28.469336878843276 37.14414098326347 18.072867615829225 16.313654522064027 7 24.38030518469275 36.574766556485024 21.48357431721234 17.561353941609884 8 24.275047858228845 35.360921832585454 20.40576391581876 19.95826639336694 9 24.806258809915114 20.37498691977668 21.2533623868176 33.565391883490605 10-11 22.540525734264445 29.742980515023838 26.546779639107832 21.169714111603888 12-13 26.325362945545088 28.117238259092492 25.655948859841622 19.901449935520805 14-15 22.950098178617374 30.494463218412033 25.182969241470154 21.37246936150044 16-17 19.648957583144057 32.52851488683299 27.999987689201582 19.822539840821378 18-19 20.145705025807704 32.043717663425696 28.53774818945962 19.272829121306977 20-21 22.38462816937822 30.355856770881424 26.32976922556062 20.929745834179734 22-23 20.876665445827037 31.711565865323948 25.73550458122636 21.676264107622657 24-25 21.90275700330545 31.282046547128818 25.973322499830726 20.84187394973501 26-27 21.363236262687817 30.21808579395417 27.30319649880893 21.115481444549086 28-29 20.575915102382513 31.452499007414385 27.18084140482412 20.790744485378983 30-31 21.834432072092035 30.884407758265166 26.585169181147243 20.69599098849556 32-33 19.73143579973716 31.497046901786018 28.009651814485725 20.761865483991098 34-35 20.24449245655827 31.621516813472937 27.446001760444172 20.687988969524618 36-37 20.862866516679645 31.049160247078483 27.107538232841 20.980435003400867 38-39 21.47526452828098 30.982894145599815 26.941566795314507 20.600274530804697 40-41 20.942206956832184 31.260502649899358 27.446001760444172 20.351288632824282 42-43 20.5307185197496 32.01115358336565 26.50176352187321 20.956364375011542 44-45 21.027523952270887 30.78847828830132 26.64649741318552 21.537500346242272 46-47 20.712918336318705 31.262041499701464 27.388448777845486 20.63659138613435 48-49 20.58679601252012 30.881117331503123 27.910820301800154 20.621266354176605 50-51 19.04172745123385 31.91666820551647 28.28959922195754 20.752005121292143 52-53 19.158372266233325 32.23213241494777 26.452212558245463 22.157282760573437 54-55 19.56450148500377 31.048735823215303 26.502008217533817 22.88475447424711 56-57 21.039770034285574 30.22393342320216 25.592303288829797 23.143993253682467 58-59 21.822121273675204 29.78289906991918 26.290325559064133 22.104654097341484 60-61 21.301990040564082 29.812752756079995 27.564493195206175 21.320764008149748 62-63 19.991813319052806 30.244554010550356 27.462621338306896 22.301011332089942 64-65 19.30825623695825 31.77632510356459 26.576859392215884 22.338559267261278 66-67 21.253670156778018 30.636960710086854 25.19281788020362 22.91655125293151 68-69 21.838500293921218 29.243776102820107 26.560321559044308 22.357402044214368 70-71 21.950461347170673 29.964483346567444 25.541521245360364 22.54353406090152 72-73 22.11050172658948 29.20059830480306 27.054210600828515 21.634689367778947 74-75 22.03294369656344 30.287949574969687 26.08750515514684 21.591601573320037 76-77 21.07331695997144 29.081183560159797 27.70298967739553 22.14250980247324 78-79 21.820341810370035 29.119128885222995 27.675683328357703 21.38484597604927 80-81 22.156051680731753 29.31878196960464 27.057903840353564 21.467262509310043 82-83 20.8294477017066 29.581275102719175 27.800501669667447 21.788775525906775 84-85 21.451874011289 29.37356502255954 26.96126407278144 22.213296893370018 86-87 21.37304025065094 29.604449177320767 26.365130465292353 22.657380106735935 88-89 32.39356633761482 18.340130350268705 33.544993711171244 15.72130960094523 90-91 34.255557732470166 14.965129996540094 17.543801143019124 33.23551112797062 92-93 25.200930299568807 17.232152964300784 35.66731499490599 21.899601741224416 94-95 20.137116993188524 7.960218322202747 31.753788467085265 40.14887621752346 96-97 34.17495006182821 8.525159326548083 18.26904784552459 39.03084276609911 98-99 57.79140562957734 14.382775963998942 19.01085326039001 8.814965146033707 100-101 34.72824147249273 7.72638046193851 13.494875847652068 44.05050221791669 102-103 27.776466267967688 2.724661630468996 38.08558913020669 31.413282971356626 104-105 46.11954482321694 5.013523721338239 39.798728039182244 9.06820341626258 106-107 10.254183506463143 3.8027322221851096 11.07251411202779 74.87057015932396 108-109 22.505031705915734 11.51458754110842 23.734142636085174 42.24623811689067 110-111 44.06277596952035 3.081270658289809 27.469028334973384 25.38692503721646 112-113 31.320476678626346 3.293182983085597 41.17375704766786 24.212583290620195 114-115 34.715383553448994 4.806497630113663 26.99139478164834 33.48672403478901 116-117 25.53076859858827 5.5528668352950845 24.449598670453195 44.46676589566345 118-119 26.757891293681514 5.399334896145669 20.134111105053698 47.70866270511912 120-121 31.091713986265585 5.0510738811456815 32.690412501759816 31.166799630828912 122-123 38.17493499209708 2.1235914954367 37.44072808851272 22.2607454239535 124-125 43.79553524258843 2.6163183566154338 21.379602668534147 32.208543732261994 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 62.0 1 132.0 2 260.0 3 366.5 4 416.5 5 417.0 6 359.5 7 295.5 8 258.5 9 254.0 10 248.0 11 195.5 12 159.5 13 121.0 14 94.0 15 98.5 16 108.0 17 105.0 18 98.5 19 121.0 20 181.0 21 224.5 22 309.0 23 462.5 24 641.0 25 805.0 26 967.5 27 1224.0 28 1520.5 29 1866.5 30 2383.0 31 2945.0 32 3480.5 33 4205.5 34 4838.5 35 5093.5 36 5304.0 37 5474.0 38 5424.0 39 5395.5 40 5462.0 41 5527.5 42 5745.5 43 6438.5 44 7515.5 45 8430.5 46 9020.0 47 8945.5 48 8007.5 49 7212.0 50 6768.0 51 6120.0 52 5199.0 53 4042.5 54 2950.5 55 2096.5 56 1491.5 57 1124.5 58 845.5 59 641.5 60 510.5 61 380.5 62 286.5 63 218.5 64 150.5 65 90.5 66 61.5 67 50.5 68 41.0 69 34.0 70 29.5 71 22.0 72 17.0 73 13.0 74 12.0 75 11.5 76 8.0 77 6.0 78 6.5 79 5.5 80 4.5 81 3.5 82 1.5 83 0.0 84 0.0 85 1.0 86 1.5 87 1.5 88 3.0 89 3.0 90 2.5 91 3.5 92 2.0 93 1.0 94 3.0 95 2.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0 3 0.040010094854701805 4 0.04678103398395903 5 0.05232089327153312 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.077699604207831E-4 12-13 0.0021543897229454817 14-15 0.0 16-17 0.0 18-19 0.004616549406311746 20-21 0.0036932395250493973 22-23 0.0015388498021039155 24-25 0.0 26-27 0.0 28-29 0.0027699296437870475 30-31 0.0 32-33 0.0015388498021039155 34-35 0.0 36-37 3.077699604207831E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 3.077699604207831E-4 46-47 0.0 48-49 3.077699604207831E-4 50-51 0.0 52-53 0.0 54-55 9.233098812623493E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.077699604207831E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.077699604207831E-4 80-81 0.0 82-83 9.233098812623493E-4 84-85 0.0 86-87 0.0012310798416831324 88-89 19.250395484399142 90-91 26.168756424697925 92-93 40.18613927206249 94-95 44.51400045549954 96-97 48.230630497540915 98-99 93.02408607710252 100-101 51.709662130137446 102-103 53.06631211567227 104-105 74.73885718858297 106-107 81.57135030992436 108-109 12.685354458663417 110-111 39.01076579321552 112-113 51.96326457752417 114-115 86.62370198019192 116-117 66.29580386435963 118-119 71.77287808000787 120-121 80.3251897401806 122-123 75.85483106506872 124-125 49.8993592229424 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 162459.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.976634104604855 #Duplication Level Percentage of deduplicated Percentage of total 1 71.22501539667432 31.322364411943937 2 12.107384804882146 10.648840630559095 3 5.696769497788478 7.5157424334755225 4 3.327081350428308 5.852553567361611 5 2.098146800291137 4.613471706707538 6 1.3199148983819495 3.4827248721215813 7 0.9238004591008342 2.8437944342880357 8 0.7124461116398858 2.506478557666857 9 0.5164884384972845 2.0442080771148414 >10 1.9315827781199262 13.034673363741007 >50 0.044790325289737414 1.3535722859306039 >100 0.07978276692234477 7.866600188355216 >500 0.009797883657130059 2.64497503985621 >1k 0.006998488326521471 4.2700004308779445 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1727 1.063037443293385 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1498 0.9220788014206661 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1399 0.8611403492573512 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1303 0.8020485168565606 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1010 0.6216953200499818 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 791 0.4868920773856788 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 680 0.418567146172265 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 638 0.3927144694969192 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 594 0.3656307129798903 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 546 0.33608479677949515 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 530 0.32623615804603007 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 518 0.31884967899593125 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 498 0.30653888057909995 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 453 0.2788395841412295 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 428 0.2634510861201903 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 426 0.26222000627850717 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 423 0.26037338651598246 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 408 0.251140287703359 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 398 0.24498488849494332 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 396 0.2437538086532602 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 388 0.2388294892865277 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 343 0.2111301928486572 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 303 0.18650859601499456 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 283 0.17419779759816323 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 279 0.17173563791479696 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 274 0.16865793831058912 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 269 0.1655802387063813 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 261 0.16065591933964876 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 260 0.16004037941880722 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 253 0.15573159997291625 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 247 0.15203836044786684 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 245 0.1508072806061837 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 242 0.14896066084365903 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 238 0.14649850116029275 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 227 0.13972756203103553 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 227 0.13972756203103553 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 211 0.12987892329757045 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 208 0.12803230353504577 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 205 0.12618568377252107 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 202 0.12433906400999638 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 198 0.1218769043266301 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 190 0.11695258495989759 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 190 0.11695258495989759 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 185 0.11387488535568975 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 184 0.11325934543484817 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 184 0.11325934543484817 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 175 0.10771948614727408 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 163 0.1003330070971753 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.233098812623493E-4 0.0 20-21 0.0 0.0 0.0 0.0012310798416831324 0.0 22-23 0.0 0.0 0.0 0.004616549406311747 0.0 24-25 0.0 0.0 0.0 0.012618568377252108 0.0 26-27 0.0 0.0 0.0 0.025237136754504213 0.0 28-29 0.0 0.0 0.0 0.05601413279658252 0.0 30-31 0.0 0.0 0.0 0.13788094226851083 0.0 32-33 0.0 0.0 0.0 0.2526791375054629 0.0 34-35 0.0 0.0 0.0 0.3988698687053349 0.0 36-37 0.0 0.0 0.0 0.6189253904061949 0.0 38-39 0.0 0.0 0.0 0.8943795049827956 0.0 40-41 0.0 0.0 0.0 1.2221545128309297 0.0 42-43 0.0 0.0 0.0 1.495454237684585 0.0 44-45 0.0 0.0 0.0 1.7761404415883393 0.0 46-47 0.0 0.0 0.0 2.057134415452514 0.0 48-49 0.0 0.0 0.0 2.365212145833718 0.0 50-51 0.0 0.0 0.0 2.6172757434183396 0.0 52-53 0.0 0.0 0.0 2.8807268295385295 0.0 54-55 0.0 0.0 0.0 3.141715755975354 0.0 56-57 0.0 0.0 0.0 3.4430225472273004 0.0 58-59 0.0 0.0 0.0 3.7150911922392726 0.0 60-61 0.0 0.0 0.0 3.957306151090429 0.0 62-63 0.0 0.0 0.0 4.19736672021864 0.0 64-65 0.0 0.0 0.0 4.474667454557766 0.0 66-67 0.0 0.0 0.0 4.792593823672434 0.0 68-69 0.0 0.0 0.0 5.1748441145150466 0.0 70-71 0.0 0.0 0.0 5.606645368985406 0.0 72-73 0.0 0.0 0.0 5.987664579986335 0.0 74-75 0.0 0.0 0.0 6.385918908770829 0.0 76-77 0.0 0.0 0.0 6.802947205140989 0.0 78-79 0.0 0.0 0.0 6.9035879821985855 0.0 80-81 0.0 0.0 0.0 6.90512683200069 0.0 82-83 0.0 0.0 0.0 6.90512683200069 0.0 84-85 0.0 0.0 0.0 6.90512683200069 0.0 86-87 0.0 0.0 0.0 6.90512683200069 0.0 88-89 0.0 0.0 0.0 6.90512683200069 0.0 90-91 0.0 0.0 0.0 6.90512683200069 0.0 92-93 0.0 0.0 0.0 6.90512683200069 0.0 94-95 0.0 0.0 0.0 6.90512683200069 0.0 96-97 0.0 0.0 0.0 6.90512683200069 0.0 98-99 0.0 0.0 0.0 6.90512683200069 0.0 100-101 0.0 0.0 0.0 6.90512683200069 0.0 102-103 0.0 0.0 0.0 6.90512683200069 0.0 104-105 0.0 0.0 0.0 6.90512683200069 0.0 106-107 0.0 0.0 0.0 6.90512683200069 0.0 108-109 0.0 0.0 0.0 6.90512683200069 0.0 110-111 0.0 0.0 0.0 6.90512683200069 0.0 112-113 0.0 0.0 0.0 6.90512683200069 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGGT 5 2.0920804E-4 6187.3335 110-111 CGTGTGG 5 2.0920804E-4 6187.3335 110-111 CATCGCG 5 3.1380454E-4 5062.3633 108-109 GGTAGCG 5 3.1380454E-4 5062.3633 108-109 CGGTGTT 10 8.36746E-4 3093.6667 110-111 ATGTTGC 10 8.36746E-4 3093.6667 110-111 CCGCTCC 30 1.204171E-9 2531.182 108-109 CAGCACG 10 0.0012550589 2531.1816 108-109 TATTGCG 10 0.0012550589 2531.1816 108-109 GGACGCG 10 0.0012550589 2531.1816 108-109 AAGGACG 10 0.0012550589 2531.1816 108-109 GGTCGCT 15 0.0018824846 2062.4443 110-111 CGTCTCT 15 0.0018824846 2062.4443 110-111 TGGTGCG 15 0.0018824846 2062.4443 110-111 CCTCGCG 30 4.2977554E-6 1687.4546 108-109 CATCGCC 15 0.0028235242 1687.4546 108-109 CGACTCC 15 0.0028235242 1687.4546 108-109 CCGTTAT 15 0.0028235242 1687.4546 108-109 CCGCACC 15 0.0028235242 1687.4546 108-109 CGGGTCT 20 0.003346295 1546.8334 110-111 >>END_MODULE