##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939902_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 162459 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.701303098012424 34.0 31.0 34.0 31.0 34.0 2 32.78454871690703 34.0 31.0 34.0 31.0 34.0 3 32.79011319779144 34.0 31.0 34.0 31.0 34.0 4 36.23091364590451 37.0 35.0 37.0 35.0 37.0 5 36.151176604558685 37.0 37.0 37.0 35.0 37.0 6 36.3253374697616 37.0 37.0 37.0 35.0 37.0 7 36.30708055570944 37.0 37.0 37.0 35.0 37.0 8 36.356822336712646 37.0 37.0 37.0 35.0 37.0 9 38.159769541853635 39.0 39.0 39.0 37.0 39.0 10-11 38.161382256446245 39.0 39.0 39.0 37.0 39.0 12-13 38.155380742218036 39.0 39.0 39.0 37.0 39.0 14-15 39.56142780640039 41.0 39.5 41.0 37.0 41.0 16-17 39.53403935762254 41.0 39.0 41.0 36.5 41.0 18-19 39.63671757181812 41.0 40.0 41.0 37.0 41.0 20-21 39.66445995605045 41.0 40.0 41.0 37.0 41.0 22-23 39.604343249681456 41.0 40.0 41.0 36.5 41.0 24-25 39.54259844022184 41.0 40.0 41.0 36.0 41.0 26-27 39.377005890717044 41.0 39.0 41.0 36.0 41.0 28-29 39.2502200555217 41.0 39.0 41.0 35.0 41.0 30-31 39.028333302556334 41.0 39.0 41.0 35.0 41.0 32-33 38.671960309985906 40.5 38.0 41.0 35.0 41.0 34-35 38.52021433100044 40.0 38.0 41.0 35.0 41.0 36-37 38.478499190565 40.5 38.0 41.0 35.0 41.0 38-39 38.45355751297251 41.0 38.0 41.0 35.0 41.0 40-41 38.35040225533827 41.0 38.0 41.0 35.0 41.0 42-43 38.143627007429565 41.0 38.0 41.0 35.0 41.0 44-45 37.83627561415496 41.0 38.0 41.0 33.5 41.0 46-47 37.36503363925668 41.0 38.0 41.0 33.0 41.0 48-49 36.93323546248592 41.0 38.0 41.0 31.5 41.0 50-51 36.65152746231357 40.0 38.0 41.0 30.5 41.0 52-53 36.55023113524028 40.0 38.0 41.0 30.0 41.0 54-55 36.417089850362245 40.0 37.0 41.0 30.0 41.0 56-57 36.21548206008901 40.0 37.0 41.0 29.0 41.0 58-59 36.037375583993494 40.0 37.0 41.0 28.0 41.0 60-61 35.72784517939911 40.0 36.0 41.0 28.0 41.0 62-63 35.43777506940212 39.0 36.0 41.0 27.0 41.0 64-65 35.16482004690414 39.0 35.0 41.0 26.0 41.0 66-67 34.752657593608234 38.5 35.0 41.0 26.0 41.0 68-69 34.30833625714796 37.5 35.0 40.0 23.0 41.0 70-71 33.77300734339126 37.0 35.0 39.5 21.5 41.0 72-73 33.32952314122332 36.5 35.0 39.0 20.0 41.0 74-75 32.95496094399202 36.0 34.0 39.0 19.5 40.5 76-77 32.52156544112668 35.0 34.0 37.0 18.0 39.5 78-79 32.140093808283936 35.0 34.0 37.0 15.0 39.0 80-81 31.910826731667683 35.0 34.0 36.5 13.0 39.0 82-83 31.708871161339168 35.0 34.0 36.0 12.0 37.5 84-85 31.457269218695178 35.0 34.0 36.0 8.5 37.0 86-87 31.271068392640604 35.0 34.0 35.5 7.0 37.0 88-89 31.079241531709542 35.0 34.0 35.0 4.0 36.0 90-91 30.91747456281277 35.0 34.0 35.0 2.0 36.0 92-93 30.759683981804642 35.0 33.0 35.0 2.0 36.0 94-95 30.69522464129411 35.0 33.0 35.0 2.0 36.0 96-97 30.62847549227805 35.0 33.0 35.0 2.0 35.5 98-99 30.54948940963566 35.0 33.0 35.0 2.0 35.0 100-101 30.472313014360545 35.0 33.0 35.0 2.0 35.0 102-103 29.533509993290615 34.0 31.0 35.0 2.0 35.0 104-105 30.1834432072092 35.0 33.0 35.0 2.0 35.0 106-107 30.18103644611871 35.0 33.0 35.0 2.0 35.0 108-109 30.157756726312485 35.0 33.0 35.0 2.0 35.0 110-111 30.096341230710518 35.0 33.0 35.0 2.0 35.0 112-113 29.948750146190733 35.0 33.0 35.0 2.0 35.0 114-115 29.77543872607858 35.0 33.0 35.0 2.0 35.0 116-117 29.600382250290842 35.0 33.0 35.0 2.0 35.0 118-119 29.425904382028698 35.0 32.5 35.0 2.0 35.0 120-121 29.24521879366486 35.0 32.0 35.0 2.0 35.0 122-123 28.9663515102272 35.0 32.0 35.0 2.0 35.0 124-125 26.916446611144966 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08070664077877154 1101 2 -0.010464623279190732 1101 3 -0.052754905508123784 1101 4 -0.022370543085109773 1101 5 -0.056919375959076035 1101 6 0.07146650568056145 1101 7 0.13777399423843661 1101 8 0.10002586166762484 1101 9 0.17833423316231745 1101 10-11 0.14559563736462877 1101 12-13 0.17436212759653102 1101 14-15 0.21356228402635935 1101 16-17 0.15683701214902612 1101 18-19 0.0391709445224393 1101 20-21 0.1585409532672415 1101 22-23 0.03981128701384762 1101 24-25 0.16537689926823873 1101 26-27 0.017619880000943056 1101 28-29 0.19710255585390968 1101 30-31 0.3378159394835265 1101 32-33 0.0792062143389316 1101 34-35 0.5394653854947222 1101 36-37 0.3905355418976626 1101 38-39 0.34243650190882846 1101 40-41 0.30227845101916273 1101 42-43 0.3500590508174142 1101 44-45 0.3142203499448115 1101 46-47 0.5069726306062563 1101 48-49 0.7273316110231676 1101 50-51 0.27276322987270163 1101 52-53 -0.0968111934562117 1101 54-55 0.12786019761319523 1101 56-57 0.15165409841126376 1101 58-59 0.09913166795902129 1101 60-61 -0.04786368707865307 1101 62-63 -0.07938863540650232 1101 64-65 -0.18347606225874102 1101 66-67 -0.0337476593599888 1101 68-69 -0.10425972785591853 1101 70-71 0.17273390428358226 1101 72-73 0.049892884221414135 1101 74-75 -0.03303765207490983 1101 76-77 0.019348183418919973 1101 78-79 -0.10231218727373914 1101 80-81 -0.0867525780190519 1101 82-83 0.09806947119667697 1101 84-85 0.0365998069850626 1101 86-87 -0.0919105146859387 1101 88-89 -0.010388018608129101 1101 90-91 0.04248346523357327 1101 92-93 0.020196067381043292 1101 94-95 0.06783571660596266 1101 96-97 0.1697013260020377 1101 98-99 -0.052341584444789646 1101 100-101 0.06225266969238419 1101 102-103 -0.06862869342323208 1101 104-105 0.0546850236553702 1101 106-107 0.18751135684158626 1101 108-109 0.2469741856330394 1101 110-111 0.27432760380357735 1101 112-113 0.49712721154301676 1101 114-115 0.7136781697947328 1101 116-117 0.5245531460243598 1101 118-119 0.3787947448041642 1101 120-121 0.4032409651962716 1101 122-123 0.3870386853002721 1101 124-125 0.7739418916564027 1102 1 0.1468902156579972 1102 2 0.2554290965275712 1102 3 0.13681031188318116 1102 4 0.026614964161261412 1102 5 0.07882941631145712 1102 6 -0.20843687596195082 1102 7 -0.22116320382919952 1102 8 -0.01852486297006095 1102 9 0.07514582736521191 1102 10-11 0.3127453958187374 1102 12-13 0.06629449474628757 1102 14-15 0.14443184924375174 1102 16-17 0.29905923437124216 1102 18-19 0.15467819089924717 1102 20-21 0.12153612234936872 1102 22-23 0.28106732566118353 1102 24-25 0.45928994274649426 1102 26-27 0.17177446937292018 1102 28-29 0.22038758000849867 1102 30-31 -0.17174927790777872 1102 32-33 -0.03929620112000265 1102 34-35 -0.0765732618482673 1102 36-37 0.012322981511189823 1102 38-39 -0.1531190536467264 1102 40-41 -0.21124811902915042 1102 42-43 -0.24757379942413138 1102 44-45 -0.07776032638368946 1102 46-47 -0.10587761093962911 1102 48-49 -0.11464906530533625 1102 50-51 0.15677289170844944 1102 52-53 0.04768156016697844 1102 54-55 0.014478555101113955 1102 56-57 -0.1259787518302815 1102 58-59 -0.08603741416657584 1102 60-61 -0.09829846968735012 1102 62-63 0.016408466042776126 1102 64-65 0.08633070102627727 1102 66-67 0.34808808943228087 1102 68-69 0.21245524798948168 1102 70-71 0.5104633728826045 1102 72-73 0.7685402272165902 1102 74-75 0.6555130725627691 1102 76-77 0.6612322413899321 1102 78-79 0.7550791170740894 1102 80-81 0.6839720596621035 1102 82-83 0.5174414518730082 1102 84-85 0.4563099519125977 1102 86-87 0.5212295819324204 1102 88-89 0.5579694693145747 1102 90-91 0.5021936101611075 1102 92-93 0.6492781929849087 1102 94-95 0.5348405475238387 1102 96-97 0.6321167849392353 1102 98-99 0.5726342609658417 1102 100-101 0.7087744088228192 1102 102-103 0.5911780698617939 1102 104-105 0.3380666661674461 1102 106-107 0.4249026611894138 1102 108-109 0.4935925431209647 1102 110-111 0.6095449951079246 1102 112-113 0.513455713958475 1102 114-115 0.5407796190700971 1102 116-117 0.7599637740436798 1102 118-119 0.7842053728234859 1102 120-121 0.8472989362107626 1102 122-123 0.8469903761215285 1102 124-125 1.1227824713665449 1103 1 0.32887748927522864 1103 2 0.3740977210992824 1103 3 0.22562088457040375 1103 4 0.182973260783875 1103 5 0.15597087495071094 1103 6 0.15170017739096409 1103 7 0.11726068261231148 1103 8 0.18106397697120258 1103 9 0.32842042354729983 1103 10-11 0.27319571397831055 1103 12-13 0.31978446048294984 1103 14-15 0.34293405188692105 1103 16-17 0.1308458227217102 1103 18-19 0.3898030073456482 1103 20-21 0.409661428272031 1103 22-23 0.2753600324649028 1103 24-25 0.5097745242442926 1103 26-27 0.4595179838124679 1103 28-29 0.5708623719810859 1103 30-31 0.5563564489644648 1103 32-33 0.4968369364228309 1103 34-35 0.49644680667840646 1103 36-37 0.5827879839585677 1103 38-39 0.5270179997061817 1103 40-41 0.45238379482303515 1103 42-43 0.46248004296450773 1103 44-45 0.37802038825165596 1103 46-47 0.6125462755018702 1103 48-49 0.4380479488894764 1103 50-51 0.4848394604798614 1103 52-53 0.5714994749981059 1103 54-55 0.44721472739676216 1103 56-57 0.6006389672103509 1103 58-59 0.5119165750649373 1103 60-61 0.5794107814139693 1103 62-63 0.4621647068650958 1103 64-65 0.5756601185495285 1103 66-67 0.563379327807219 1103 68-69 0.4329936718759271 1103 70-71 0.5546435001464403 1103 72-73 0.40679769172955815 1103 74-75 0.27380969517657405 1103 76-77 0.3715699800096104 1103 78-79 0.1948147998934715 1103 80-81 0.249581457606304 1103 82-83 0.38800434957246566 1103 84-85 0.47475341740452137 1103 86-87 0.48110231810471404 1103 88-89 0.4685911041652808 1103 90-91 0.6370247408512704 1103 92-93 0.5771391817269524 1103 94-95 0.7085654618654935 1103 96-97 0.7464682288942015 1103 98-99 0.8378422883765069 1103 100-101 0.6119462149902191 1103 102-103 0.9114325975172015 1103 104-105 1.0888547229466923 1103 106-107 0.5780304144933766 1103 108-109 0.5892362044033916 1103 110-111 0.21113089819162667 1103 112-113 0.2143269817019906 1103 114-115 0.43860634447391433 1103 116-117 0.3090484534367306 1103 118-119 0.26879666014605164 1103 120-121 0.502570595535289 1103 122-123 0.36145929437899227 1103 124-125 0.09814223641793873 1104 1 0.13204218376961308 1104 2 0.20638076568552322 1104 3 0.12263990829573856 1104 4 0.12754172051403145 1104 5 -0.026601575218194284 1104 6 0.03034737914517649 1104 7 -0.023056577023019997 1104 8 -0.042321574479771584 1104 9 0.1088080097269497 1104 10-11 0.07049327823029472 1104 12-13 0.05713654756143427 1104 14-15 0.11501480888501447 1104 16-17 -0.22029303269402334 1104 18-19 0.07901301900089663 1104 20-21 0.10402740286755119 1104 22-23 0.1882222833094147 1104 24-25 0.04915541359851261 1104 26-27 0.16586515198378038 1104 28-29 0.26272938369559284 1104 30-31 0.31354220191285265 1104 32-33 0.4799763449686836 1104 34-35 0.49483680292502186 1104 36-37 0.4809527772261859 1104 38-39 0.24647237634829366 1104 40-41 0.30604136776706525 1104 42-43 0.4264969529425855 1104 44-45 0.17577230939109967 1104 46-47 -0.01880237426798459 1104 48-49 -0.08084737123458297 1104 50-51 -0.15699890699607977 1104 52-53 -0.1290299495490146 1104 54-55 0.024652944737390214 1104 56-57 -0.1705627079837413 1104 58-59 -0.1476318336982203 1104 60-61 -0.1939935369519219 1104 62-63 0.07386735991423166 1104 64-65 -0.06200014102653739 1104 66-67 -0.33519043572965757 1104 68-69 -0.5411870041281048 1104 70-71 -0.5546885952290026 1104 72-73 -0.006726339798852621 1104 74-75 -0.3662507540441098 1104 76-77 -0.15377523245161484 1104 78-79 -0.006445546603046637 1104 80-81 -0.0016482691869192934 1104 82-83 -0.14003737224258828 1104 84-85 0.08103341977507839 1104 86-87 0.03068648277945485 1104 88-89 0.263300809623491 1104 90-91 -0.231468572200626 1104 92-93 -0.03962066102605277 1104 94-95 -0.247381674698385 1104 96-97 -0.34078804814496877 1104 98-99 0.008947165798922185 1104 100-101 -0.13436460463010036 1104 102-103 -0.11374470044214391 1104 104-105 -0.09659202840156311 1104 106-107 -0.15025428948323238 1104 108-109 -0.15085190132348103 1104 110-111 -0.2808585923360205 1104 112-113 -0.29864194423783985 1104 114-115 -0.29691843773110094 1104 116-117 -0.32139148255821226 1104 118-119 0.10482321039199505 1104 120-121 0.2609910139387175 1104 122-123 0.45109600641550074 1104 124-125 0.002124773309741812 1105 1 0.20498814409491217 1105 2 0.24550442609442769 1105 3 0.07697980340860511 1105 4 0.0148070545567478 1105 5 0.025062937968705512 1105 6 0.15440681518682453 1105 7 -0.034628363904531057 1105 8 0.020401690702257724 1105 9 0.26249046013357713 1105 10-11 0.3005514221840926 1105 12-13 0.2632530634845125 1105 14-15 0.2612962560234138 1105 16-17 0.367853961301563 1105 18-19 0.18244655248116715 1105 20-21 0.20723292084277034 1105 22-23 0.22015395466306842 1105 24-25 0.15327299359395852 1105 26-27 0.4217650531770616 1105 28-29 0.42512392652449904 1105 30-31 0.26486089158375137 1105 32-33 0.16986575744873278 1105 34-35 0.16671299050578625 1105 36-37 0.3300160135262544 1105 38-39 0.32633480699357875 1105 40-41 0.2190249506506987 1105 42-43 -0.1882423493299683 1105 44-45 -0.13478480849291685 1105 46-47 -0.07112243203191326 1105 48-49 -0.6017582931772765 1105 50-51 -0.5758918917190456 1105 52-53 -0.5731376488725672 1105 54-55 -0.6686947029026484 1105 56-57 -0.849782894956455 1105 58-59 -1.13139522960914 1105 60-61 -1.0342306730771753 1105 62-63 -0.5089022684204991 1105 64-65 -0.6925770202543262 1105 66-67 -0.8328817787409832 1105 68-69 -0.5339760137807588 1105 70-71 -0.6636515047068414 1105 72-73 -0.5201697539510164 1105 74-75 -0.8754473794146307 1105 76-77 -0.9760276456157158 1105 78-79 -0.7480564761462531 1105 80-81 -0.9053782228237672 1105 82-83 -0.6609577948313223 1105 84-85 -1.01815332492356 1105 86-87 -0.8361809642069389 1105 88-89 -0.7347800598756358 1105 90-91 -0.5101510226072534 1105 92-93 -0.5999402627589703 1105 94-95 -0.5122121831729594 1105 96-97 -0.7491638176973012 1105 98-99 -0.5232885262281357 1105 100-101 -0.45071711660091296 1105 102-103 -0.41586524463915 1105 104-105 -0.6445227876931945 1105 106-107 -0.243826152369909 1105 108-109 -0.5385637657302631 1105 110-111 -0.5066414455700397 1105 112-113 -0.7702825157967048 1105 114-115 -0.4859717933592833 1105 116-117 -0.6922301995909734 1105 118-119 -0.7125648719976034 1105 120-121 -1.2666558660491205 1105 122-123 -1.370956893181674 1105 124-125 -1.2226130088594438 1106 1 0.1899457712135586 1106 2 0.245151318749798 1106 3 0.2543103118831809 1106 4 0.1524482974946011 1106 5 0.18021830520034143 1106 6 0.23795201292693946 1106 7 0.16078124061524335 1106 8 0.20147513702994502 1106 9 0.43431249403187877 1106 10-11 0.2592731735965188 1106 12-13 0.3374056058573984 1106 14-15 0.2681818492437529 1106 16-17 0.254753678815689 1106 18-19 0.3580115242325803 1106 20-21 0.35709167790491847 1106 22-23 0.22703954788340752 1106 24-25 0.2559566094131611 1106 26-27 0.3452466915951433 1106 28-29 0.19094313556404785 1106 30-31 0.26408405542555613 1106 32-33 0.30348157665777364 1106 34-35 0.2799545159295036 1106 36-37 0.3246840926222987 1106 38-39 0.43618650190882846 1106 40-41 0.3109741031930753 1106 42-43 0.4227039783536455 1106 44-45 0.4262674513940894 1106 46-47 0.41231683350481063 1106 48-49 0.5685453791391097 1106 50-51 0.6652451139306734 1106 52-53 0.8426815601669801 1106 54-55 0.7696174439900076 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-0.46898203180911224 2216 28-29 -0.6088795594714114 2216 30-31 -0.8278485686879193 2216 32-33 -0.9665538843351342 2216 34-35 -0.8668096684676527 2216 36-37 -1.035998531491174 2216 38-39 -1.205790448445775 2216 40-41 -0.9900897265941637 2216 42-43 -0.9011435393768537 2216 44-45 -0.974308789740661 2216 46-47 -1.1268232374171774 2216 48-49 -0.9704173868183403 2216 50-51 -1.2229197796863502 2216 52-53 -1.2046588653649337 2216 54-55 -1.7015261730312687 2216 56-57 -1.447420832208529 2216 58-59 -1.166841196672486 2216 60-61 -1.6321991789072072 2216 62-63 -1.3583433070068693 2216 64-65 -1.4510215448365997 2216 66-67 -1.370611674161104 2216 68-69 -1.6145896692681987 2216 70-71 -1.483957430899899 2216 72-73 -1.228953862617928 2216 74-75 -1.4834230976499931 2216 76-77 -1.3501861983263765 2216 78-79 -1.3952045708691756 2216 80-81 -1.2133329048769 2216 82-83 -1.3208327798054817 2216 84-85 -1.5248247998604505 2216 86-87 -1.4888886213772707 2216 88-89 -1.5332834857681696 2216 90-91 -1.4622035529594584 2216 92-93 -1.370059868480812 2216 94-95 -1.5807150080317207 2216 96-97 -1.6407673758172656 2216 98-99 -1.744694344235107 2216 100-101 -1.7439915486239919 2216 102-103 -1.6627699206819422 2216 104-105 -1.6372052014448464 2216 106-107 -1.6277214522857655 2216 108-109 -1.8175185679901453 2216 110-111 -1.69378833822541 2216 112-113 -1.740846886514337 2216 114-115 -2.0080147071710392 2216 116-117 -1.7593506467601046 2216 118-119 -1.6739033742214318 2216 120-121 -2.0595095744275334 2216 122-123 -1.9717803094576674 2216 124-125 -2.060409017995159 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 0.0 4 6.0 5 22.0 6 53.0 7 109.0 8 197.0 9 382.0 10 618.0 11 849.0 12 1028.0 13 1672.0 14 2682.0 15 2963.0 16 1101.0 17 750.0 18 670.0 19 637.0 20 732.0 21 903.0 22 865.0 23 490.0 24 610.0 25 686.0 26 809.0 27 956.0 28 1236.0 29 1576.0 30 1927.0 31 2412.0 32 3161.0 33 4052.0 34 5780.0 35 9636.0 36 20062.0 37 56001.0 38 36501.0 39 312.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11379040747604 19.747105067132892 15.131834103878994 21.007270421512075 2 14.38393687022572 22.597086034014737 39.50104334016583 23.51793375559372 3 20.249416775925003 27.69621873826627 31.374685305215472 20.679679180593258 4 12.378507808123896 19.34026431284201 37.40328328993777 30.877944589096323 5 11.221571648690293 39.19322033898305 35.16795069337442 14.417257318952235 6 27.771314608608943 36.828984543792586 17.956530570790168 17.443170276808303 7 23.147994263167938 35.710548507623464 22.627247490135975 18.514209739072626 8 24.896127638357985 33.02556337291255 21.17518881687072 20.903120171858745 9 25.236521214583373 16.96551129823525 22.626016410294287 35.17195107688709 10-11 22.931324211031708 27.183473984205246 27.84948817855582 22.03571362620723 12-13 27.564800965166597 25.43257067937141 27.598040120892044 19.404588234569953 14-15 23.59733840538228 27.05636499055146 28.443176432207512 20.903120171858745 16-17 18.49974455093285 29.789362239088018 31.34914039850055 20.36175281147859 18-19 19.210385389544438 27.097606165247846 34.553333456441315 19.138674988766397 20-21 20.669914775612714 25.69196723985633 33.23566435933975 20.402453625191207 22-23 19.127713008900763 27.055915990594492 32.927279666129095 20.889091334375653 24-25 20.683680190078725 26.783373035658226 32.843979096264285 19.688967677998757 26-27 20.350057552982598 25.479659483315793 34.46531124776097 19.704971715940637 28-29 19.347035251971267 26.169987504539606 34.472389956850655 20.010587286638476 30-31 20.535950609076753 25.58553234970054 34.07259677826405 19.805920262958654 32-33 18.55852861337322 26.054881539342233 35.49664838513102 19.88994146215353 34-35 18.95555186231603 26.264780652349206 34.63058371650693 20.14908376882783 36-37 19.80776688272118 25.462116595571803 34.52409531020134 20.20602121150567 38-39 20.77170239875907 25.37286330704978 33.95718304310626 19.89825125108489 40-41 20.146006069223617 25.739725099871354 34.23971586677254 19.874552964132487 42-43 20.182322924553272 26.223231707692403 33.00771271520814 20.586732652546182 44-45 20.461778048615344 25.53136483666648 32.70302045439157 21.303836660326606 46-47 20.815713503099243 25.767424396309224 32.915073957121486 20.501788143470044 48-49 20.992373460380772 25.736955170227567 32.54021014532897 20.730461224062687 50-51 19.117131091536944 27.237025957318462 32.645775241753306 21.000067709391292 52-53 19.413513563422153 27.71222277620815 30.81454397724964 22.05971968312005 54-55 19.46614222665411 26.61471509734764 30.515083805760224 23.40405887023803 56-57 21.01145519792686 26.03395318203362 29.3981866193932 23.556405000646315 58-59 21.5288165013942 26.05765146898602 30.118983866698674 22.294548162921107 60-61 21.0505419829003 26.542389156648756 31.161092952683443 21.245975907767498 62-63 19.750521670082914 27.23548710751636 30.873020269729594 22.140970952671136 64-65 19.05773148917573 28.87159221711324 29.940785059615045 22.12989123409599 66-67 20.667676152136846 27.563569885324913 28.942071538049603 22.826682424488638 68-69 21.181651986039554 26.356803870515023 30.106365298321425 22.355178845124 70-71 21.27398297416579 26.964957312306492 29.12704128426249 22.63401842926523 72-73 21.221046540973415 25.998559636585235 30.644962729057795 22.135431093383563 74-75 21.02345822638327 27.041899802411685 29.659483315790446 22.275158655414597 76-77 19.962575172812834 25.864371933841767 31.266965819068194 22.906087074277202 78-79 20.377449079460046 26.105663582811662 31.332828590598243 22.184058747130045 80-81 20.595965751358804 26.29709649819339 30.658196837355888 22.448740913091918 82-83 19.23377590653642 26.62210157639774 31.061991025427954 23.082131491637888 84-85 19.95826639336694 26.354341710831658 30.15560849198875 23.531783403812653 86-87 19.629568075637547 26.857237826159214 29.595774933983343 23.917419164219893 88-89 20.27219175299614 25.39409943431881 30.419367348069358 23.91434146461569 90-91 20.462218884556076 25.671645465989144 30.557598850146654 23.30853679930812 92-93 19.804996953077392 26.51715201989425 30.788075760653456 22.8897752663749 94-95 18.906616438609124 26.549160095778014 30.492924368609923 24.051299097002936 96-97 19.609563028210193 26.190608091887803 30.38428157258139 23.815547307320617 98-99 20.761197750754825 25.81245825754288 29.453169964513144 23.973174027189152 100-101 19.306469036707835 26.13590547739884 29.743903827746777 24.81372165814654 102-103 20.68596191015524 25.444422558445872 29.515320882935896 24.354294648462986 104-105 21.470385462961822 25.182820983170622 29.272594088172653 24.074199465694903 106-107 19.774023471765247 26.337253501835946 30.29569007174493 23.59303295465388 108-109 19.31883933078088 26.55086237673737 30.001908185500252 24.128390106981495 110-111 18.641986328143034 27.795543873364505 29.4320423759853 24.130427422507164 112-113 19.202570684592512 26.600983705454702 28.273221419909262 25.92322419004352 114-115 18.66285833882217 27.474130660937764 28.066924795784576 25.7960862044555 116-117 19.991259171714187 26.554267001526565 28.039284483183142 25.415189343576106 118-119 19.4686643050379 25.541599854732134 30.09784037154104 24.891895468688926 120-121 18.99302584683947 27.30137821084964 29.212960968133107 24.492634974177783 122-123 18.768601018727978 27.25882153794069 28.891864025976023 25.08071341735531 124-125 18.63833480553136 27.169286358418148 28.65523174038429 25.537147095666196 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 534.0 1 334.5 2 172.0 3 192.5 4 113.0 5 75.5 6 159.5 7 353.0 8 560.5 9 624.0 10 433.5 11 225.0 12 170.5 13 153.0 14 162.5 15 178.5 16 203.0 17 222.5 18 241.5 19 275.0 20 311.0 21 380.5 22 451.0 23 488.5 24 580.0 25 757.0 26 939.5 27 1042.5 28 1301.0 29 1577.5 30 1865.5 31 2230.5 32 2599.5 33 3182.5 34 3973.5 35 4810.0 36 5494.0 37 5838.5 38 5329.5 39 4649.5 40 4725.5 41 4882.0 42 4947.0 43 5040.5 44 5119.5 45 5191.0 46 5414.5 47 6746.5 48 10000.0 49 10881.5 50 11769.0 51 11275.5 52 7002.5 53 4561.0 54 3155.0 55 2148.5 56 1513.0 57 1226.5 58 1022.5 59 860.0 60 687.5 61 467.0 62 284.5 63 208.0 64 170.0 65 93.0 66 48.0 67 25.0 68 17.0 69 12.0 70 9.5 71 7.0 72 6.0 73 4.5 74 3.0 75 5.0 76 6.0 77 3.0 78 1.5 79 1.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012310798416831324 2 0.0 3 0.0 4 0.0 5 0.1286478434558873 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0033854695646286137 22-23 6.155399208415662E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0021543897229454817 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0027699296437870475 100-101 3.077699604207831E-4 102-103 6.155399208415662E-4 104-105 0.003077699604207831 106-107 0.005232089327153313 108-109 6.155399208415662E-4 110-111 0.005232089327153313 112-113 0.0073864790500987945 114-115 0.00492431936673253 116-117 0.0018466197625246986 118-119 3.077699604207831E-4 120-121 0.0012310798416831324 122-123 9.233098812623493E-4 124-125 0.0021543897229454817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 162459.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.540437895099686 #Duplication Level Percentage of deduplicated Percentage of total 1 68.55083518844279 27.105300414258366 2 13.124834596883417 10.37923414523049 3 6.256518828712425 7.421564825586763 4 3.480859940532715 5.505389052006968 5 2.241698709466507 4.431887430059277 6 1.502249482385541 3.563976141672668 7 1.060136681351869 2.934278802651746 8 0.7519031088002242 2.378446254131812 9 0.5806622351604216 2.0663675142651377 >10 2.2588227968304873 13.90443127189014 >50 0.06849634945592105 1.8995561957170732 >100 0.09807431853915967 8.970878806344986 >500 0.015567352149072964 3.9271446949691926 >1k 0.00934041128944378 5.511544451215383 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1745 1.074117161868533 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1664 1.0242584282803662 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1564 0.9627044361962095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1511 0.9300808203916066 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1448 0.8913018053785878 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1022 0.6290817991000807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 892 0.549061609390677 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 799 0.49181639675241134 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 696 0.42841578490573007 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 682 0.4197982260139481 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 586 0.36070639361315776 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 583 0.3588597738506331 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 559 0.3440868157504355 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 543 0.33423817701697045 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 529 0.3256206181251885 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 511 0.31454089955004033 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 466 0.28684160311216983 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 460 0.2831483635871204 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 427 0.26283554619934874 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 427 0.26283554619934874 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 423 0.26037338651598246 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 412 0.25360244738672527 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 400 0.24621596833662648 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 400 0.24621596833662648 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 395 0.24313826873241864 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 381 0.2345207098406367 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 372 0.2289808505530626 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 355 0.218516671898756 RNA PCR Primer, Index 27 (95% over 23bp) ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 330 0.20312817387771687 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 308 0.1895862956192024 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 303 0.18650859601499456 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 279 0.17173563791479696 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 277 0.17050455807311385 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 263 0.1618869991813319 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 263 0.1618869991813319 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 258 0.15880929957712409 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 251 0.1545005201312331 No Hit ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 248 0.15265390036870843 RNA PCR Primer, Index 27 (96% over 25bp) GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 247 0.15203836044786684 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 232 0.14280526163524335 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 231 0.14218972171440178 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 224 0.13788094226851083 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 221 0.13603432250598613 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 220 0.13541878258514456 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 213 0.13111000313925358 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 212 0.13049446321841204 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 212 0.13049446321841204 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 204 0.1255701438516795 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 201 0.1237235240891548 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 199 0.12249244424747166 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 199 0.12249244424747166 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 197 0.12126136440578854 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 186 0.1144904252765313 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 186 0.1144904252765313 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 178 0.10956610590979877 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 177 0.10895056598895722 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 177 0.10895056598895722 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 170 0.10464178654306625 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 169 0.10402624662222469 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 165 0.10156408693885843 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 165 0.10156408693885843 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 163 0.1003330070971753 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.233098812623493E-4 0.0 20-21 0.0 0.0 0.0 0.0012310798416831324 0.0 22-23 0.0 0.0 0.0 0.004616549406311747 0.0 24-25 0.0 0.0 0.0 0.012618568377252108 0.0 26-27 0.0 0.0 0.0 0.025237136754504213 0.0 28-29 0.0 0.0 0.0 0.05570636283616174 0.0 30-31 0.0 0.0 0.0 0.13788094226851083 0.0 32-33 0.0 0.0 0.0 0.2514480576637798 0.0 34-35 0.0 0.0 0.0 0.39794655882407254 0.0 36-37 0.0 0.0 0.0 0.61707877064367 0.0 38-39 0.0 0.0 0.0 0.8913018053785878 0.0 40-41 0.0 0.0 0.0 1.2209234329892464 0.0 42-43 0.0 0.0 0.0 1.4932998479616395 0.0 44-45 0.0 0.0 0.0 1.7767559815091807 0.0 46-47 0.0 0.0 0.0 2.057134415452514 0.0 48-49 0.0 0.0 0.0 2.3670587655962425 0.0 50-51 0.0 0.0 0.0 2.6188145932204434 0.0 52-53 0.0 0.0 0.0 2.882881219261475 0.0 54-55 0.0 0.0 0.0 3.1447934555795616 0.0 56-57 0.0 0.0 0.0 3.447639096633612 0.0 58-59 0.0 0.0 0.0 3.720938821487268 0.0 60-61 0.0 0.0 0.0 3.9646926301405276 0.0 62-63 0.0 0.0 0.0 4.206599819031263 0.0 64-65 0.0 0.0 0.0 4.479899543884919 0.0 66-67 0.0 0.0 0.0 4.803058002326741 0.0 68-69 0.0 0.0 0.0 5.1930025421798724 0.0 70-71 0.0 0.0 0.0 5.629728116016965 0.0 72-73 0.0 0.0 0.0 6.01320948670126 0.0 74-75 0.0 0.0 0.0 6.413310435248278 0.0 76-77 0.0 0.0 0.0 6.831262041499702 0.0 78-79 0.0 0.0 0.0 7.29445583193298 0.0 80-81 0.0 0.0 0.0 7.7795012895561335 0.0 82-83 0.0 0.0 0.0 8.278704165358644 0.0 84-85 0.0 0.0 0.0 8.808684037203232 0.0 86-87 0.0 0.0 0.0 9.374980764377474 0.0 88-89 0.0 0.0 0.0 9.966206858345798 0.0 90-91 0.0 0.0 0.0 10.585132248751993 0.0 92-93 0.0 0.0 0.0 11.204057639158187 0.0 94-95 0.0 0.0 0.0 11.808825611385025 0.0 96-97 0.0 0.0 0.0 12.474224265814758 0.0 98-99 0.0 0.0 0.0 13.186096184268031 0.0 100-101 0.0 0.0 0.0 14.05092977305043 0.0 102-103 0.0 0.0 0.0 14.839744181608898 0.0 104-105 0.0 0.0 0.0 15.586701875550139 0.0 106-107 0.0 0.0 0.0 16.353664616918728 0.0 108-109 0.0 0.0 0.0 17.155713133775293 0.0 110-111 0.0 0.0 0.0 18.054401418203977 0.0 112-113 0.0 0.0 0.0 19.01772139432103 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGACT 35 0.0077842786 51.0 4 TCCTCGA 30 0.0013071282 39.666668 86-87 TAGGACT 70 0.0027819772 34.0 4 CGTTTCC 35 0.002786057 34.0 106-107 GTAATGG 75 0.0039006942 31.733332 3 AGTACTT 1405 0.0 30.91459 12-13 CGTTCCA 40 0.005356611 29.750002 22-23 GAGTACT 1470 0.0 28.738096 12-13 AGTAGCA 85 0.007192442 28.000002 2 CTTAAGA 150 5.2577598E-6 27.766665 2 GTACTTT 1520 0.0 27.597038 14-15 TACTTTT 1575 0.0 27.2 14-15 GCTCTAG 90 0.009505356 26.444443 1 ACTTTTT 1785 0.0 23.666666 16-17 ACCAGGA 190 3.283166E-5 21.921051 2 TCACTAT 155 0.0056676087 19.19355 3 CATGGGG 255 1.4836134E-5 18.666666 4 CTTCATT 115 1.4321692E-4 18.108696 48-49 AAGTTTG 100 0.0011718719 17.85 20-21 ACCAGGC 235 1.6818913E-4 17.723404 7 >>END_MODULE