FastQCFastQC Report
Fri 27 May 2016
SRR939901_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939901_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464291
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29790.203443168058808No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23050.15741406591995716No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21560.14723849289519636No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21210.1448482576209237No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA20700.1413653433641264No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20320.13877023078063033No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA19200.1311214779029578No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA17480.11937517884081784No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA16630.11357032174615564No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16000.10926789825246484No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG15240.10407767308547276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC7050.01429.0648108-109
CCGCGCC6400.01220.0084108-109
CCGCGCG2400.01049.4695108-109
CGTCACG300.008800151963.38403110-111
CCGCTCG2300.0876.0789108-109
CGTCGCT1009.6229996E-8867.04565110-111
CCTCGCC6050.0666.10956108-109
CTCCGCC5250.0660.6062110-111
TTGTTCG2900.0607.96857108-109
CGCGTCT1454.24865E-7597.9625110-111
GGTCGCA851.0405647E-4592.64166108-109
CGTCGTC1606.2962135E-7541.9035110-111
CCTCGCG2850.0530.2583108-109
CGTGAGT1101.4908907E-4525.4822110-111
AATCGCG502.3159242E-4503.74542108-109
CGGCGCG1559.626001E-7487.4955108-109
CGTTGCG1201.9350831E-4481.69202110-111
GGTGGCG5400.0466.43094108-109
CGTCGCG1101.2474447E-7457.95038108-109
CGTCGCC3400.0444.48123108-109
Produced by FastQC (version 0.11.5)