##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939901_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1464291 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.772537699132208 33.0 31.0 34.0 30.0 34.0 2 31.778916212692696 34.0 31.0 34.0 30.0 34.0 3 31.96235447735457 34.0 31.0 34.0 30.0 34.0 4 35.27120360638698 37.0 35.0 37.0 33.0 37.0 5 35.22112066522296 37.0 35.0 37.0 33.0 37.0 6 35.320384404466054 37.0 36.0 37.0 33.0 37.0 7 35.23627065931567 37.0 36.0 37.0 33.0 37.0 8 35.17990754569959 37.0 36.0 37.0 33.0 37.0 9 36.84973068877703 39.0 38.0 39.0 33.0 39.0 10-11 36.84995537089281 39.0 38.0 39.0 34.0 39.0 12-13 36.808807812108384 39.0 38.0 39.0 33.0 39.0 14-15 38.305883871443584 41.0 39.0 41.0 34.0 41.0 16-17 38.287735156468216 41.0 39.0 41.0 34.0 41.0 18-19 38.281302008958605 41.0 39.0 41.0 33.5 41.0 20-21 38.33938540904779 41.0 39.0 41.0 34.0 41.0 22-23 38.314454230750584 41.0 39.0 41.0 34.0 41.0 24-25 38.324144244552485 41.0 39.0 41.0 34.0 41.0 26-27 38.29286596721553 41.0 39.0 41.0 34.0 41.0 28-29 38.2425740511961 41.0 39.0 41.0 33.0 41.0 30-31 38.169466656559386 40.0 38.0 41.0 33.0 41.0 32-33 38.065991322763026 40.0 38.0 41.0 33.0 41.0 34-35 37.975051748593685 40.0 38.0 41.0 33.0 41.0 36-37 38.13743955265723 40.5 38.0 41.0 33.0 41.0 38-39 38.10162324292098 41.0 38.0 41.0 33.0 41.0 40-41 38.04886699433378 41.0 38.0 41.0 33.0 41.0 42-43 37.9258313409015 40.5 38.0 41.0 33.0 41.0 44-45 37.78006215977562 40.0 38.0 41.0 33.0 41.0 46-47 37.66655603291969 40.0 38.0 41.0 33.0 41.0 48-49 37.53736176757216 40.0 38.0 41.0 32.0 41.0 50-51 37.38609231361799 40.0 37.0 41.0 32.0 41.0 52-53 37.22244827018673 40.0 37.0 41.0 31.5 41.0 54-55 37.03267451619931 40.0 36.5 41.0 31.0 41.0 56-57 36.81324204000434 40.0 36.0 41.0 31.0 41.0 58-59 36.59113625638619 39.0 35.5 41.0 31.0 41.0 60-61 36.30699259914867 39.0 35.0 41.0 31.0 41.0 62-63 36.00967772116335 39.0 35.0 41.0 30.0 41.0 64-65 35.6727610836917 38.0 35.0 40.5 30.0 41.0 66-67 35.33789117053919 37.0 35.0 40.0 30.0 41.0 68-69 34.95426216510243 37.0 35.0 39.5 29.5 41.0 70-71 34.55627262613784 36.0 34.0 39.0 29.0 41.0 72-73 34.174122151949305 36.0 34.0 39.0 29.0 41.0 74-75 33.774603203871365 35.0 34.0 37.5 29.0 40.0 76-77 33.376458299613944 35.0 34.0 37.0 28.0 39.0 78-79 32.97910046568613 35.0 34.0 37.0 27.5 39.0 80-81 32.620281760934134 35.0 34.0 36.0 27.0 38.5 82-83 32.49300207404129 35.0 34.0 36.0 27.0 37.0 84-85 32.168047539730836 35.0 34.0 36.0 27.0 37.0 86-87 31.591519376954444 35.0 34.0 35.0 14.0 37.0 88-89 16.26537996887231 18.0 16.5 18.5 2.0 19.0 90-91 2.725105528887359 2.0 2.0 2.0 2.0 2.0 92-93 2.28111079013666 2.0 2.0 2.0 2.0 2.0 94-95 2.0977124765500843 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.025020837128408857 1101 2 -0.0012708224215103314 1101 3 0.06104886198012238 1101 4 0.0034242827494068706 1101 5 0.002629627421995906 1101 6 0.05075498666149514 1101 7 -0.028892144849336887 1101 8 0.012740079101803303 1101 9 0.05981870633080888 1101 10-11 0.08019497314082713 1101 12-13 0.1536592582036107 1101 14-15 0.17324030455580441 1101 16-17 0.14356209797769282 1101 18-19 0.07214347707590463 1101 20-21 -0.02413263878933236 1101 22-23 0.04825035253431764 1101 24-25 0.1091614773780023 1101 26-27 0.12233992431045948 1101 28-29 0.15436286491840434 1101 30-31 0.09616765139202954 1101 32-33 0.2063876287859756 1101 34-35 0.2481076130044002 1101 36-37 0.10506051004196593 1101 38-39 0.08361786786416303 1101 40-41 0.08086899817866566 1101 42-43 0.07942184338541125 1101 44-45 0.05179810107901517 1101 46-47 0.09310091935569176 1101 48-49 0.10501474222880347 1101 50-51 -0.02978385823045926 1101 52-53 -0.0356335405985746 1101 54-55 -0.0036526557487803757 1101 56-57 -0.07451795631030933 1101 58-59 -0.09319559181741965 1101 60-61 -0.056753264540802206 1101 62-63 -0.06505110058434127 1101 64-65 -0.02309412807006339 1101 66-67 -0.018043293078306988 1101 68-69 -0.0125572329174517 1101 70-71 -0.01639584864995669 1101 72-73 3.7540055211593426E-4 1101 74-75 -0.05205484110291536 1101 76-77 -0.06826587612692947 1101 78-79 0.0605942859745241 1101 80-81 0.0459858997065794 1101 82-83 0.02491625905341266 1101 84-85 0.08439009271040021 1101 86-87 0.037124910424680024 1101 88-89 0.5131985522128026 1101 90-91 -0.14006945076467892 1101 92-93 -0.17017790444245406 1101 94-95 -0.0024028989082021113 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.10198886478735503 1102 2 -0.06338835520441322 1102 3 -0.02079119426032605 1102 4 -0.03348506500773851 1102 5 0.049009148446742756 1102 6 0.0596902547017919 1102 7 0.01582566603105562 1102 8 0.052362762454322365 1102 9 0.04716327444806012 1102 10-11 0.03452865908581515 1102 12-13 0.08386332942673391 1102 14-15 0.014599544866193526 1102 16-17 0.003255924612517447 1102 18-19 -0.08194426778405983 1102 20-21 -0.029157687522925357 1102 22-23 -0.03757942696142891 1102 24-25 -0.032973619457145276 1102 26-27 0.08053903235821025 1102 28-29 0.011735442376540561 1102 30-31 0.01304732654576668 1102 32-33 -0.006115780902192114 1102 34-35 -0.054215188550649884 1102 36-37 -0.03219291128903734 1102 38-39 -0.03172650509695529 1102 40-41 -0.051919339468057046 1102 42-43 -0.05378774321622615 1102 44-45 -0.052233425507679954 1102 46-47 -0.15270987677045866 1102 48-49 -0.15784072469334376 1102 50-51 -0.18411066935701115 1102 52-53 -0.06995740220626345 1102 54-55 -0.17688510666590673 1102 56-57 -0.23451682529179863 1102 58-59 -0.17943375770456527 1102 60-61 -0.21047032362654505 1102 62-63 -0.2516287937944597 1102 64-65 -0.2513418699139791 1102 66-67 -0.30622747611702295 1102 68-69 -0.34866255957111747 1102 70-71 -0.23940941421908946 1102 72-73 -0.24974801352706777 1102 74-75 -0.20841870528312256 1102 76-77 -0.24533503014725255 1102 78-79 -0.2643548736343675 1102 80-81 -0.38051508383598787 1102 82-83 -0.3241204901776058 1102 84-85 -0.439774006987772 1102 86-87 -0.41892881073711763 1102 88-89 0.6315974513547822 1102 90-91 0.16949974206510676 1102 92-93 -0.03127774232980007 1102 94-95 0.08900612839369737 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.21296536469449734 1103 2 -0.1117615752051897 1103 3 -0.11300821919435222 1103 4 -0.15888425561288955 1103 5 -0.18508045510527182 1103 6 -0.15291669641921857 1103 7 -0.2345326013283895 1103 8 -0.24596100412553312 1103 9 -0.17726628358088448 1103 10-11 -0.17385099633829526 1103 12-13 -0.14718370099252098 1103 14-15 -0.21226230084776887 1103 16-17 -0.17251859370253442 1103 18-19 -0.18343974787095618 1103 20-21 -0.10735715109367305 1103 22-23 -0.15810777953624466 1103 24-25 -0.1938291852345131 1103 26-27 -0.15492446928951864 1103 28-29 -0.11896865340328588 1103 30-31 -0.035869665131379236 1103 32-33 -0.2029619915608123 1103 34-35 -0.22977828809239043 1103 36-37 -0.07004874916819404 1103 38-39 -0.11107678648475883 1103 40-41 -0.11304268796135375 1103 42-43 -0.09722793049136413 1103 44-45 -0.1122372694853766 1103 46-47 -0.20919151421858118 1103 48-49 -0.27918603050552804 1103 50-51 -0.22765796971852126 1103 52-53 -0.28560735836764195 1103 54-55 -0.34616740579647143 1103 56-57 -0.3711870767915926 1103 58-59 -0.3408134433622081 1103 60-61 -0.3791928704993168 1103 62-63 -0.45116665265568656 1103 64-65 -0.40235102203615014 1103 66-67 -0.40166588393282154 1103 68-69 -0.47018753894753473 1103 70-71 -0.45350493490530397 1103 72-73 -0.5005086028218102 1103 74-75 -0.4252816739568601 1103 76-77 -0.4181868296902991 1103 78-79 -0.4234506318697697 1103 80-81 -0.4642392935530424 1103 82-83 -0.42791976543664134 1103 84-85 -0.394088548021152 1103 86-87 -0.4349571179616376 1103 88-89 0.8601595013610428 1103 90-91 -0.4261321603583266 1103 92-93 -0.29653595829224155 1103 94-95 -0.10230842559691267 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.05372787024869652 1104 2 -0.08462370380394901 1104 3 0.009274916691094148 1104 4 0.11930468939166872 1104 5 0.10614881182382874 1104 6 0.05364445610066326 1104 7 0.09780963332689652 1104 8 0.13082400610671385 1104 9 0.21177634972512038 1104 10-11 0.054093668188045285 1104 12-13 0.0921016482284287 1104 14-15 0.029314820647869055 1104 16-17 0.08455323226796452 1104 18-19 0.08445092879222926 1104 20-21 0.017838575771122578 1104 22-23 -0.06857971440035016 1104 24-25 0.0321057456222249 1104 26-27 0.027113718570724643 1104 28-29 0.08186734490281111 1104 30-31 0.04627845937448427 1104 32-33 0.11609845690560405 1104 34-35 0.08236939833575008 1104 36-37 -0.02041590001504545 1104 38-39 -0.035277972632329124 1104 40-41 0.06932718731166432 1104 42-43 0.06486384887600849 1104 44-45 -0.025322755136244268 1104 46-47 -0.02717731632844078 1104 48-49 -0.051330233172791395 1104 50-51 -0.0764616003397407 1104 52-53 -0.004189780603631732 1104 54-55 -0.0715818586061161 1104 56-57 -0.03180691503661848 1104 58-59 -0.11236467655098181 1104 60-61 -0.12892230535820204 1104 62-63 -0.09952250526966111 1104 64-65 -0.1832635587773197 1104 66-67 -0.20277182155473383 1104 68-69 -0.19462883340460024 1104 70-71 -0.1830681922843027 1104 72-73 -0.16212704964856073 1104 74-75 -0.20678509409216161 1104 76-77 -0.2361305972003649 1104 78-79 -0.1894482912831137 1104 80-81 -0.1783065948065783 1104 82-83 -0.18371615943122066 1104 84-85 -0.18499134274836493 1104 86-87 -0.15191082983583826 1104 88-89 0.711024809152363 1104 90-91 -0.5829562638114218 1104 92-93 -0.29653595829224155 1104 94-95 -0.10230842559691267 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.09379672593711419 1105 2 -0.2165664222535284 1105 3 -0.04199863738547549 1105 4 0.0824398938568578 1105 5 5.6433510302156265E-5 1105 6 -0.10180002209656891 1105 7 -0.07226698550286415 1105 8 -0.08695696284379295 1105 9 -0.03940085548262573 1105 10-11 -0.04965565292063445 1105 12-13 -0.06462021958951425 1105 14-15 -0.04857648923648128 1105 16-17 -1.0796644696853264E-4 1105 18-19 0.05400775468000063 1105 20-21 0.03187390573531701 1105 22-23 -0.032515166795107575 1105 24-25 0.011185153659681646 1105 26-27 -0.04504115149638466 1105 28-29 -0.06315703381574878 1105 30-31 -0.0381486177260868 1105 32-33 -0.11744659846431205 1105 34-35 0.030843925719999277 1105 36-37 -0.04684187861577982 1105 38-39 -0.01725827941449154 1105 40-41 -0.042787915833784496 1105 42-43 -0.0718269663765696 1105 44-45 -0.16123111938406254 1105 46-47 -0.13581895069982153 1105 48-49 -0.09434386623088642 1105 50-51 -0.18349506776369395 1105 52-53 -0.13850930904649061 1105 54-55 -0.1785429109566934 1105 56-57 -0.10986576148903993 1105 58-59 -0.3376433671882637 1105 60-61 -0.3157912432806853 1105 62-63 -0.17283877810265835 1105 64-65 -0.17766090273971002 1105 66-67 -0.2597327204835409 1105 68-69 -0.26939893088878364 1105 70-71 -0.3319084771578389 1105 72-73 -0.3047246028782382 1105 74-75 -0.2911167428545127 1105 76-77 -0.34069673578931514 1105 78-79 -0.29706752300828043 1105 80-81 -0.43611775716055234 1105 82-83 -0.3207978933426503 1105 84-85 -0.3672060779884241 1105 86-87 -0.27301153895123775 1105 88-89 0.6118407061020292 1105 90-91 -0.7260401640381793 1105 92-93 -0.29653595829224155 1105 94-95 -0.10230842559691267 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.2430450786135232 1106 2 -0.28114675746985895 1106 3 -0.22277345821442296 1106 4 -0.20160737913944615 1106 5 -0.2479454184608798 1106 6 -0.290825183607069 1106 7 -0.41423370252596214 1106 8 -0.3401006600560521 1106 9 -0.4365985557926422 1106 10-11 -0.45420187225035136 1106 12-13 -0.33448565335886826 1106 14-15 -0.4087237281049596 1106 16-17 -0.4393192796997809 1106 18-19 -0.3558425960251057 1106 20-21 -0.35474267895298084 1106 22-23 -0.3529649499760623 1106 24-25 -0.48134222615907873 1106 26-27 -0.5307912952866687 1106 28-29 -0.31963177326297654 1106 30-31 -0.3465975799301262 1106 32-33 -0.29369620169857313 1106 34-35 -0.5059651947607477 1106 36-37 -0.3741762930611543 1106 38-39 -0.38316786933174285 1106 40-41 -0.3937176602569963 1106 42-43 -0.8228030697432942 1106 44-45 -0.5287934026545074 1106 46-47 -0.6372995133554014 1106 48-49 -0.5243335085570209 1106 50-51 -0.5504096980973614 1106 52-53 -0.653276452309349 1106 54-55 -0.6672923650309031 1106 56-57 -0.7944486383926161 1106 58-59 -0.5443311668508031 1106 60-61 -0.5339109674897173 1106 62-63 -0.4914852162799832 1106 64-65 -0.520086436076383 1106 66-67 -0.6048252355129051 1106 68-69 -0.40104102791202223 1106 70-71 -0.40176937646167943 1106 72-73 -0.3522631040703317 1106 74-75 -0.47753339341188905 1106 76-77 -0.48616209127948196 1106 78-79 -0.5033182837245391 1106 80-81 -0.4456031430555072 1106 82-83 -0.4306423373097843 1106 84-85 -0.46622717041276474 1106 86-87 -0.5823656043382321 1106 88-89 0.5260915766000807 1106 90-91 -0.23022887656022517 1106 92-93 -0.2711832246061743 1106 94-95 -0.10230842559691267 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.25372248550904786 1107 2 -0.28425993472551525 1107 3 -0.21329432208620958 1107 4 -0.23873981248999598 1107 5 -0.23474349936626027 1107 6 -0.16824092074136843 1107 7 -0.21852467524261954 1107 8 -0.30803586501851754 1107 9 -0.22267581284615545 1107 10-11 -0.13162330989605664 1107 12-13 -0.2168148823239875 1107 14-15 -0.21587600854221023 1107 16-17 -0.17555661642084885 1107 18-19 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2215 84-85 0.4045646569993764 2215 86-87 0.4633466234074497 2215 88-89 -0.7395985679695478 2215 90-91 -0.7260401640381793 2215 92-93 -0.29653595829224155 2215 94-95 -0.10230842559691267 2215 96-97 0.0 2215 98-99 0.0 2215 100-101 0.0 2215 102-103 0.0 2215 104-105 0.0 2215 106-107 0.0 2215 108-109 0.0 2215 110-111 0.0 2215 112-113 0.0 2215 114-115 0.0 2215 116-117 0.0 2215 118-119 0.0 2215 120-121 0.0 2215 122-123 0.0 2215 124-125 0.0 2216 1 0.5944769672418566 2216 2 0.6296998033767061 2216 3 0.5790783936374275 2216 4 0.5289040847053528 2216 5 0.515304140622014 2216 6 0.5454622943470753 2216 7 0.5326357860101893 2216 8 0.523567770279044 2216 9 0.5572506505565613 2216 10-11 0.5475177044692288 2216 12-13 0.49306064293742224 2216 14-15 0.5729230972918629 2216 16-17 0.6042763993125675 2216 18-19 0.5579911782253291 2216 20-21 0.5323935644332565 2216 22-23 0.6003639741862017 2216 24-25 0.5695726768391509 2216 26-27 0.5775199921912773 2216 28-29 0.6548170803525508 2216 30-31 0.6418944835619342 2216 32-33 0.5489735513878458 2216 34-35 0.6172049992427802 2216 36-37 0.617291960907103 2216 38-39 0.5855270160298005 2216 40-41 0.5516637330410674 2216 42-43 0.5973468420732857 2216 44-45 0.6211625056347287 2216 46-47 0.6834566594841007 2216 48-49 0.6609288371219932 2216 50-51 0.6796608486398554 2216 52-53 0.7221754877259201 2216 54-55 0.7900489048103623 2216 56-57 0.7715897213957348 2216 58-59 0.7662398208035199 2216 60-61 0.733152524573768 2216 62-63 0.6714997925383557 2216 64-65 0.6805308478742305 2216 66-67 0.6676835828292482 2216 68-69 0.7348826934283537 2216 70-71 0.6300317699228017 2216 72-73 0.6596416578344275 2216 74-75 0.6967390933605984 2216 76-77 0.7360160392319841 2216 78-79 0.6880838326775773 2216 80-81 0.7130719010362085 2216 82-83 0.7207575745067984 2216 84-85 0.8617159512803525 2216 86-87 0.9536683462790556 2216 88-89 -0.28360859976676167 2216 90-91 -0.7260401640381793 2216 92-93 -0.29653595829224155 2216 94-95 -0.10230842559691267 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores warn #Quality Count 2 18792.0 3 3181.0 4 2165.0 5 1783.0 6 1872.0 7 2131.0 8 2478.0 9 2905.0 10 3363.0 11 4043.0 12 5101.0 13 5629.0 14 6048.0 15 6342.0 16 6867.0 17 7771.0 18 9931.0 19 12528.0 20 14947.0 21 19900.0 22 23503.0 23 31545.0 24 48639.0 25 86895.0 26 187426.0 27 502864.0 28 430940.0 29 14256.0 30 446.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74936402668595 18.386577531378666 13.872925531878568 23.991132910056812 2 15.253645804166672 19.78944004108487 39.70146542410431 25.255448730644147 3 19.250367356474797 24.17844336493749 28.553315703865934 28.017873574721776 4 12.588444503955879 16.57964258070422 37.19263464025452 33.639278275085374 5 13.957223598822477 37.94648133459857 33.89217155103566 14.204123515543285 6 32.530555743359756 36.191644966744995 17.432873656943872 13.844925632951375 7 29.22970912202561 31.754412203585215 20.881505110664477 18.134373563724697 8 26.707396275740276 34.541563118259965 19.70380204481213 19.047238561187633 9 26.23030531499545 17.200679373157385 19.612221887589286 36.95679342425788 10-11 25.186524735997075 26.24762426388888 27.263309073450966 21.302541926663082 12-13 26.147057779040452 23.448030005436163 27.21492923426586 23.18998298125753 14-15 22.622313460917262 25.288313593404588 24.932680730811022 27.156692214867128 16-17 23.01906867920611 28.130740852538374 25.875341292616334 22.974849175639186 18-19 23.25377615442826 27.35314727343756 26.750970503787762 22.642106068346415 20-21 23.66646133000788 26.63577283300393 26.563586298080534 23.134179538907652 22-23 23.33117186665014 26.439313152132083 26.639889065198098 23.589625916019678 24-25 23.512846831674853 26.70036215479027 26.482031235594565 23.304759777940316 26-27 22.946224486799412 26.821103182359245 26.504089692554277 23.72858263828706 28-29 23.103063596980213 27.615431658864743 26.02792302404841 23.253581720106634 30-31 23.268291616898555 26.838893362043475 26.095017998471615 23.79779702258636 32-33 22.83732416200793 26.90575670946831 26.19772665498397 24.059192473539788 34-35 23.77132390828453 26.97321806924109 25.966723110423178 23.288734912051204 36-37 23.78290927144946 26.264383240762935 26.25697351141269 23.695733976374914 38-39 23.211301578716252 26.87652249450417 26.161261661787172 23.75091426499241 40-41 23.60056846624066 26.666728129859436 26.211798064728935 23.520905339170973 42-43 23.166467594214538 26.65993303243686 25.90779428405966 24.265805089288946 44-45 23.293089725023826 26.840439106857556 26.281123861692745 23.585347306425877 46-47 23.965659831276707 27.079487615508118 25.623185555330192 23.331666997884984 48-49 23.407957642284778 27.081306612314975 25.82253999462538 23.688195750774863 50-51 23.241145373426455 27.175950681934125 26.306451381590136 23.276452563049286 52-53 23.693480325973457 26.84210310655464 25.976667206176913 23.487749361294988 54-55 22.980353725143374 26.962467710074456 26.329115652994915 23.728062911787255 56-57 23.382169254608545 26.90564921863209 26.1711640650663 23.541017461693066 58-59 23.38292047140903 26.58098697594945 26.144188552685225 23.891903999956295 60-61 22.84112242716782 26.9320783915219 26.65757694338079 23.569222237929484 62-63 23.003939450539356 26.98030206437862 26.633615392574082 23.382143092507942 64-65 22.6684185959002 27.254530436412722 26.312470100707475 23.764580866979607 66-67 22.950902518693347 27.053775513200584 26.165325061753435 23.82999690635263 68-69 23.47606719174588 26.654166840243747 26.577679102525664 23.292086865484706 70-71 23.33262309199469 26.6541623215604 26.15726655425732 23.85594803218759 72-73 23.158648110245846 26.728396199935666 26.40107055223313 23.711885137585355 74-75 23.133038446592924 27.568871214806347 25.96714041129803 23.3309499273027 76-77 23.332281629812655 26.703708484174253 26.253695474465115 23.710314411547976 78-79 23.455860584660172 26.94356603028164 26.284712725276098 23.315860659782082 80-81 23.805753091427864 26.88662977509252 26.018154861294647 23.28946227218497 82-83 23.36845972791471 26.989377398570703 26.391644787365287 23.250518086149302 84-85 23.24618074288563 27.428070942231386 26.26047435958724 23.06527395529574 86-87 23.49036624850865 27.36852061663112 25.94741166130225 23.193701473557976 88-89 32.60099572538729 17.398877956270272 31.732262378355436 18.267863939987 90-91 30.870016159427337 12.86318197182055 14.330587619748764 41.93621424900334 92-93 22.955628054609154 16.107680602780032 31.937828555166288 28.99886278744453 94-95 14.414327136615142 6.290367267703285 24.936674258556614 54.35863133712496 96-97 24.50358393255541 6.478639574206414 13.308128341600508 55.70964815163767 98-99 57.09546571768927 14.170323345949507 18.63876029776176 10.095450638599454 100-101 30.796002032961795 6.646121996345543 11.230393495669205 51.32748247502346 102-103 23.391245634045873 1.9272252329986348 35.42510061640476 39.256428516550734 104-105 43.38458685371747 4.96012807016587 36.09950528262162 15.55577979349504 106-107 4.669225483749418 1.2903678638187204 5.628781889781232 88.41162476265063 108-109 17.388258010694987 8.958730724106525 17.81404220817262 55.838969057025864 110-111 40.12155554641641 2.4494509659642247 21.547876946128085 35.881116541491274 112-113 27.70232964217839 2.7352312518263715 36.24648098168495 33.315958124310285 114-115 27.310962997012183 3.796317577057637 21.295487627365357 47.59723179856483 116-117 18.43451278203931 3.804449343928468 18.484936638443596 59.27610123558863 118-119 17.818712886292555 3.728874699238844 13.181761818457371 65.27065059601122 120-121 24.269791076293437 4.171058602102126 26.49462638974962 45.064523931854815 122-123 33.598129496402876 1.4870503597122302 31.109064748201437 33.80575539568345 124-125 38.07230991934928 1.5744123526573377 17.004204866616117 43.349072861377266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5.0 1 17.5 2 47.0 3 90.0 4 116.0 5 127.0 6 139.5 7 139.0 8 127.0 9 121.0 10 123.0 11 130.0 12 128.0 13 134.5 14 150.0 15 160.0 16 197.5 17 271.5 18 381.0 19 535.0 20 807.5 21 1285.0 22 1992.5 23 2997.5 24 4430.5 25 6130.5 26 8103.0 27 10363.0 28 12782.5 29 15232.0 30 17348.5 31 19640.0 32 22156.5 33 24923.5 34 27777.5 35 30589.0 36 33626.0 37 36531.5 38 39305.0 39 41767.5 40 44085.5 41 46140.5 42 48229.0 43 51586.5 44 54992.5 45 56773.0 46 57059.0 47 57405.0 48 58369.0 49 59099.5 50 60294.5 51 60970.5 52 59446.0 53 55838.5 54 51146.0 55 46533.5 56 42272.5 57 37930.5 58 32852.0 59 27403.5 60 22322.0 61 17936.0 62 14313.5 63 11088.0 64 8316.0 65 6036.0 66 4223.5 67 3042.0 68 2241.5 69 1607.0 70 1122.0 71 760.0 72 506.0 73 340.0 74 252.0 75 205.0 76 159.5 77 108.0 78 76.5 79 55.5 80 37.5 81 26.0 82 27.5 83 20.5 84 14.0 85 18.5 86 13.5 87 7.5 88 9.0 89 8.0 90 7.0 91 9.5 92 7.5 93 4.0 94 3.0 95 1.5 96 3.0 97 4.5 98 4.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 3.4146218203895266E-4 3 0.0310047661291369 4 0.03551206693205108 5 0.036877915660206886 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.463394912623242E-4 12-13 0.0015707260373791824 14-15 0.0 16-17 3.4146218203895266E-4 18-19 0.007034120950002424 20-21 0.0019804806558259254 22-23 5.121932730584289E-4 24-25 0.0 26-27 0.0 28-29 0.0010585327643207532 30-31 0.0 32-33 0.0011609714189324389 34-35 1.0243865461168579E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 3.414621820389526E-5 46-47 0.0 48-49 3.414621820389526E-5 50-51 0.0 52-53 0.0 54-55 0.0012634100735441248 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.414621820389526E-5 64-65 3.414621820389526E-5 66-67 0.0 68-69 2.731697456311621E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.073159638350574E-4 80-81 0.0 82-83 5.121932730584289E-4 84-85 6.829243640779052E-5 86-87 8.195092368934863E-4 88-89 18.904370784222536 90-91 26.253388158501284 92-93 40.487990433595506 94-95 44.66301438716758 96-97 49.07982771184143 98-99 93.20657574211684 100-101 52.36571828960227 102-103 53.77776685098795 104-105 74.95893917260982 106-107 81.51337404928391 108-109 12.570247307399965 110-111 38.55927544456669 112-113 52.441522894014916 114-115 86.62868241353665 116-117 66.54712075673484 118-119 72.73796670197386 120-121 80.660299079896 122-123 76.26837834829279 124-125 50.46408125160914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1464291.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.89754592993692 #Duplication Level Percentage of deduplicated Percentage of total 1 69.90151056476336 33.48110812847724 2 14.636484385928922 14.021033662556704 3 6.074700544467874 8.728897450177884 4 3.0477786813427326 5.839244774955844 5 1.7279385700780203 4.138200851221075 6 1.0912907209210554 3.136208845693813 7 0.7267510030663494 2.436671268429874 8 0.5165433578713226 1.9792927366756412 9 0.37040376686306964 1.5967288292350972 >10 1.7813559922547728 14.673967095990301 >50 0.08307539415941705 2.6658636279393284 >100 0.034047111333109836 3.132361616611281 >500 0.004273635236825649 1.367578252131648 >1k 0.0038462717131430837 2.8028428599043975 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2979 0.203443168058808 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2305 0.15741406591995716 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2156 0.14723849289519636 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2121 0.1448482576209237 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2070 0.1413653433641264 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2032 0.13877023078063033 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1920 0.1311214779029578 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1748 0.11937517884081784 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1663 0.11357032174615564 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1600 0.10926789825246484 No Hit CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG 1524 0.10407767308547276 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.829243640779052E-5 2 0.0 0.0 0.0 0.0 6.829243640779052E-5 3 0.0 0.0 0.0 0.0 6.829243640779052E-5 4 0.0 0.0 0.0 0.0 6.829243640779052E-5 5 0.0 0.0 0.0 0.0 6.829243640779052E-5 6 0.0 0.0 0.0 0.0 2.0487730922337158E-4 7 0.0 0.0 0.0 0.0 2.0487730922337158E-4 8 0.0 0.0 0.0 0.0 2.0487730922337158E-4 9 0.0 0.0 0.0 0.0 2.0487730922337158E-4 10-11 0.0 0.0 0.0 0.0 2.3902352742726684E-4 12-13 0.0 0.0 0.0 0.0 7.170705822818006E-4 14-15 0.0 0.0 0.0 0.0 8.195092368934863E-4 16-17 0.0 0.0 0.0 0.0 8.878016733012768E-4 18-19 0.0 0.0 0.0 0.0 8.878016733012768E-4 20-21 0.0 0.0 0.0 1.0243865461168579E-4 9.902403279129627E-4 22-23 0.0 0.0 0.0 2.3902352742726684E-4 0.0010243865461168579 24-25 0.0 0.0 0.0 3.4146218203895266E-4 0.0010243865461168579 26-27 0.0 0.0 0.0 6.146319276701148E-4 0.0010926789825246484 28-29 0.0 0.0 0.0 0.0017414571283986585 0.0010926789825246484 30-31 0.0 0.0 0.0 0.003687791566020689 0.0011951176371363342 32-33 0.0 0.0 0.0 0.008604846987381606 0.0012292638553402296 34-35 0.0 0.0 0.0 0.015638967937384028 0.0012975562917480201 36-37 0.0 0.0 0.0 0.02639502667161104 0.0012975562917480201 38-39 0.0 0.0 0.0 0.045448616429384595 0.0014682873827674965 40-41 0.0 0.0 0.0 0.06941926160851908 0.0015024336009713916 42-43 0.0 0.0 0.0 0.09222893536872111 0.0015024336009713916 44-45 0.0 0.0 0.0 0.12080932000538144 0.0015365798191752868 46-47 0.0 0.0 0.0 0.15205310966194563 0.0016048722555830775 48-49 0.0 0.0 0.0 0.18930663372239534 0.0017073109101947632 50-51 0.0 0.0 0.0 0.23335525520542022 0.0017756033466025537 52-53 0.0 0.0 0.0 0.27235023639426864 0.0019121882194181347 54-55 0.0 0.0 0.0 0.3130525284933118 0.00194633443762203 56-57 0.0 0.0 0.0 0.36256454488895995 0.0020146268740298206 58-59 0.0 0.0 0.0 0.4194179981984455 0.0020487730922337157 60-61 0.0 0.0 0.0 0.48549093042298286 0.0021170655286415064 62-63 0.0 0.0 0.0 0.5529297113756759 0.0021170655286415064 64-65 0.0 0.0 0.0 0.6173294789082224 0.0021170655286415064 66-67 0.0 0.0 0.0 0.6942950547398024 0.0021170655286415064 68-69 0.0 0.0 0.0 0.7780557279939575 0.0021170655286415064 70-71 0.0 0.0 0.0 0.8772163456580693 0.0021512117468454016 72-73 0.0 0.0 0.0 0.9857330271100484 0.0024243814924765636 74-75 0.0 0.0 0.0 1.0907326480870265 0.0026975512381077257 76-77 0.0 0.0 0.0 1.2064541815800274 0.0027999898927194115 78-79 0.0 3.414621820389526E-5 0.0 1.2383467493824658 0.0027999898927194115 80-81 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 82-83 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 84-85 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 86-87 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 88-89 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 90-91 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 92-93 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 94-95 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 96-97 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 98-99 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 100-101 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 102-103 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 104-105 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 106-107 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 108-109 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 110-111 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 112-113 0.0 6.829243640779052E-5 0.0 1.2388247964373202 0.0027999898927194115 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 705 0.0 1429.0648 108-109 CCGCGCC 640 0.0 1220.0084 108-109 CCGCGCG 240 0.0 1049.4695 108-109 CGTCACG 30 0.008800151 963.38403 110-111 CCGCTCG 230 0.0 876.0789 108-109 CGTCGCT 100 9.6229996E-8 867.04565 110-111 CCTCGCC 605 0.0 666.10956 108-109 CTCCGCC 525 0.0 660.6062 110-111 TTGTTCG 290 0.0 607.96857 108-109 CGCGTCT 145 4.24865E-7 597.9625 110-111 GGTCGCA 85 1.0405647E-4 592.64166 108-109 CGTCGTC 160 6.2962135E-7 541.9035 110-111 CCTCGCG 285 0.0 530.2583 108-109 CGTGAGT 110 1.4908907E-4 525.4822 110-111 AATCGCG 50 2.3159242E-4 503.74542 108-109 CGGCGCG 155 9.626001E-7 487.4955 108-109 CGTTGCG 120 1.9350831E-4 481.69202 110-111 GGTGGCG 540 0.0 466.43094 108-109 CGTCGCG 110 1.2474447E-7 457.95038 108-109 CGTCGCC 340 0.0 444.48123 108-109 >>END_MODULE