FastQCFastQC Report
Fri 27 May 2016
SRR939901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939901_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464291
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37350.25507224998309763No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31800.21716994777677387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27380.18698469088453046No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG20230.13815559885296025No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA19490.13310195855878373No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19160.13084830815732665No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC18890.1290044123743163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18190.12422394182577097No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA17920.12238004604276063No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA17500.11951176371363342No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16300.11131667134469855No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA15060.10284840923013253No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA14840.10134597562916113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105400.026.6665331
GAGTACT56150.025.1656512-13
TACATGG58400.023.7538532
GTACTTT58550.023.67762414-15
CAACGCA118050.023.6536315
AGTACTT56800.023.62068612-13
TCAACGC118650.023.5340184
ATCAACG118750.023.51423
GTACATG60200.023.4432071
TATCAAC120150.023.3393152
AACGCAG120300.023.2448376
AGAGTAC89450.022.7810910-11
ACATGGG58550.022.1676983
TAAGGTG10900.021.8486445
ACGCAGA131400.021.1906767
CGCAGAG131900.021.1103448
ACTTTTT65750.021.04098116-17
TACTTTT63150.020.7751614-15
GTGTAGC11700.020.3581981
GGTATCA84650.019.9782091
Produced by FastQC (version 0.11.5)