FastQCFastQC Report
Fri 27 May 2016
SRR939900_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939900_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634055
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA14140.2230090449566678No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13060.20597582228671016No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC12520.1974592109517313No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA8660.13658121140910487No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA8510.13421548603827743No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8000.12617201977746412No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA7310.11528968307165782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA53.135433E-45099.1313108-109
CTCCGCC650.02695.0745110-111
CCGCTCC1900.02683.7534108-109
CCGCTCG401.8189894E-122549.5657108-109
CGTCGCG100.00125401082549.5657108-109
CCGCGCA100.00125401082549.5657108-109
CGTCGTG150.00214990041946.4426110-111
CAGCGCG404.0163286E-91912.1743108-109
CAACGCG150.00282115891699.7106108-109
TAGTTCG150.00282115891699.7106108-109
GGACGCC150.00282115891699.7106108-109
CCGCGCG609.094947E-121699.7106108-109
CCGCGCC456.4319465E-91699.7104108-109
CGTGTTG354.624382E-61668.3795110-111
TTGTGCG406.901995E-61459.832110-111
CGTAGTT200.00382161351459.832110-111
CGTCGTT200.00382161351459.832110-111
CGTCGCT200.00382161351459.832110-111
CTGCGCG356.9581292E-61456.8948108-109
CTCCGCT459.826013E-61297.6284110-111
Produced by FastQC (version 0.11.5)