##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939900_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 634055 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.620569193524222 33.0 31.0 34.0 28.0 34.0 2 31.770952046746732 34.0 31.0 34.0 28.0 34.0 3 31.92780752458383 34.0 31.0 34.0 30.0 34.0 4 35.0970073574059 37.0 35.0 37.0 32.0 37.0 5 35.16533896901689 37.0 35.0 37.0 32.0 37.0 6 35.211800238149685 37.0 37.0 37.0 33.0 37.0 7 35.122997216329814 37.0 36.0 37.0 32.0 37.0 8 35.13007862093983 37.0 37.0 37.0 33.0 37.0 9 36.68651773111166 39.0 38.0 39.0 33.0 39.0 10-11 36.67079827459763 39.0 38.0 39.0 33.0 39.0 12-13 36.59539787557861 39.0 38.0 39.0 33.0 39.0 14-15 38.09774230942111 41.0 39.0 41.0 33.0 41.0 16-17 38.12567679459984 41.0 39.0 41.0 33.0 41.0 18-19 38.16477040635276 41.0 39.0 41.0 33.0 41.0 20-21 38.236733406407964 41.0 39.0 41.0 33.0 41.0 22-23 38.26364432107625 41.0 39.0 41.0 33.0 41.0 24-25 38.317050571322675 41.0 39.0 41.0 33.0 41.0 26-27 38.306796728990385 41.0 39.0 41.0 33.0 41.0 28-29 38.26365299540261 41.0 39.0 41.0 33.0 41.0 30-31 38.17736158535143 40.5 39.0 41.0 33.0 41.0 32-33 38.09001190748437 40.0 38.0 41.0 33.0 41.0 34-35 38.041411234041206 40.0 38.0 41.0 33.0 41.0 36-37 38.202551040524874 41.0 39.0 41.0 33.0 41.0 38-39 38.142574382348535 41.0 39.0 41.0 33.0 41.0 40-41 38.04725773000765 41.0 38.0 41.0 33.0 41.0 42-43 38.00236335964546 41.0 38.0 41.0 33.0 41.0 44-45 37.929688276253636 41.0 38.0 41.0 32.0 41.0 46-47 37.81772874592898 40.0 38.0 41.0 32.0 41.0 48-49 37.73490864357193 40.0 38.0 41.0 32.0 41.0 50-51 37.63953205952165 40.0 38.0 41.0 31.5 41.0 52-53 37.550186498016735 40.0 38.0 41.0 31.0 41.0 54-55 37.4161161098012 40.0 38.0 41.0 31.0 41.0 56-57 37.22022300904496 40.0 37.5 41.0 30.5 41.0 58-59 37.05874726955864 40.0 37.0 41.0 30.5 41.0 60-61 36.86000031543005 40.0 36.5 41.0 31.0 41.0 62-63 36.60845352532509 40.0 36.0 41.0 30.0 41.0 64-65 36.32606240783528 39.0 36.0 41.0 30.0 41.0 66-67 36.014372570202895 39.0 35.0 41.0 30.0 41.0 68-69 35.6011623597322 39.0 35.0 41.0 29.0 41.0 70-71 35.17400540962535 38.0 35.0 40.0 28.5 41.0 72-73 34.78273966769444 37.0 34.5 40.0 28.0 41.0 74-75 34.36107198902303 37.0 34.0 39.0 28.0 41.0 76-77 33.86418134073543 36.0 34.0 39.0 26.5 40.5 78-79 33.374909116717006 36.0 34.0 38.5 26.0 40.0 80-81 32.89681731080111 35.0 34.0 37.0 26.0 39.0 82-83 32.64271948017128 35.0 34.0 37.0 26.0 39.0 84-85 32.146568515349614 35.0 34.0 37.0 23.5 39.0 86-87 31.429267965712754 35.0 34.0 36.0 2.0 37.0 88-89 15.902372033971817 18.0 16.5 18.5 2.0 19.5 90-91 2.781575730811996 2.0 2.0 2.0 2.0 2.0 92-93 2.3082824045232666 2.0 2.0 2.0 2.0 2.0 94-95 2.102353896743973 2.0 2.0 2.0 2.0 2.0 96-97 2.0000449487820458 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17290234052640585 1101 2 0.21298426478122678 1101 3 0.19073843371569055 1101 4 0.22470942820949347 1101 5 0.20975523730277956 1101 6 0.220877810570137 1101 7 0.14407146125690673 1101 8 0.27093374475933274 1101 9 0.44907885947222326 1101 10-11 0.3455336168508154 1101 12-13 0.13505907832696096 1101 14-15 0.2371005405426203 1101 16-17 0.28296546205182693 1101 18-19 0.2393630781386875 1101 20-21 0.2807773250608889 1101 22-23 0.19555378611276097 1101 24-25 0.25657859713315645 1101 26-27 0.15108354202524765 1101 28-29 0.16295548555415706 1101 30-31 0.19737006082887376 1101 32-33 0.16802091414985654 1101 34-35 0.147760980770542 1101 36-37 0.12674097431334985 1101 38-39 0.14991315457036336 1101 40-41 0.12798863865369725 1101 42-43 0.12582421733287674 1101 44-45 0.15735349034560642 1101 46-47 0.09285751049744562 1101 48-49 0.16721436002417533 1101 50-51 0.2308979539192677 1101 52-53 0.2323545146003454 1101 54-55 0.3943477944234459 1101 56-57 0.2992821018910945 1101 58-59 0.10107863329791655 1101 60-61 -0.025689967881220355 1101 62-63 0.10189223498066013 1101 64-65 0.0027557580465895626 1101 66-67 -0.016215057359637797 1101 68-69 0.2709177427131735 1101 70-71 0.14801593275856106 1101 72-73 3.9371774021645933E-4 1101 74-75 -0.016667459357137204 1101 76-77 -0.03415979478240416 1101 78-79 -0.02902491100339688 1101 80-81 0.00632999019993008 1101 82-83 -0.008265903225343152 1101 84-85 -0.03311640506203162 1101 86-87 0.09563693097551607 1101 88-89 0.46771161017390206 1101 90-91 -0.09298702434906048 1101 92-93 -0.1732324683544384 1101 94-95 -0.009382148272930824 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.14666506824288206 1102 2 -0.0527597286622985 1102 3 -0.027437061455110268 1102 4 -0.021554827444433045 1102 5 -0.010674654488617819 1102 6 -0.15782191085259 1102 7 0.049609324541251 1102 8 -0.13836813402611625 1102 9 -0.13069382828168585 1102 10-11 -0.11747737667661795 1102 12-13 -0.030883481981796024 1102 14-15 -0.04606456421872451 1102 16-17 -0.11052645726168464 1102 18-19 -0.16210495478474485 1102 20-21 -0.2096858719750827 1102 22-23 -0.13174775949864426 1102 24-25 -0.11891766803447013 1102 26-27 -0.08971617295287615 1102 28-29 -0.07187931970317862 1102 30-31 -0.04946752168945778 1102 32-33 -0.002686399749940449 1102 34-35 -0.14241597842049458 1102 36-37 0.05256293534658596 1102 38-39 0.017064261158090233 1102 40-41 0.0032283354896449623 1102 42-43 -0.021039873193188896 1102 44-45 -0.13775845734850378 1102 46-47 0.0420162460411575 1102 48-49 0.17191953139601424 1102 50-51 0.07866842375546668 1102 52-53 0.15373516837536982 1102 54-55 0.08816753753896478 1102 56-57 0.04280253555237579 1102 58-59 0.04918312835076932 1102 60-61 -9.907060927574207E-4 1102 62-63 -0.096238707281735 1102 64-65 -0.13077711492289978 1102 66-67 -0.06796647061340622 1102 68-69 0.0651335251918681 1102 70-71 0.3413462505020206 1102 72-73 0.23345017614879282 1102 74-75 0.18613825518566784 1102 76-77 0.11469178834148153 1102 78-79 0.1807841462235018 1102 80-81 0.09627542991062654 1102 82-83 0.09523813924583635 1102 84-85 0.26453751465761854 1102 86-87 0.25576657702065475 1102 88-89 1.0378307624526766 1102 90-91 0.01904943510523882 1102 92-93 -0.05207202559775137 1102 94-95 0.0657029995821703 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.36492419658251407 1103 2 -0.09395548313448288 1103 3 -0.0794542236316147 1103 4 -0.10903224640049558 1103 5 -0.1299702075536331 1103 6 -0.17210234380219447 1103 7 -0.12904361487215965 1103 8 -0.18566791349284273 1103 9 -0.2289669979780129 1103 10-11 -0.23673386118272788 1103 12-13 -0.12975084364119738 1103 14-15 -0.17442169049880363 1103 16-17 -0.086226396734574 1103 18-19 -0.04804575483885998 1103 20-21 -0.047246967620743874 1103 22-23 -0.0636891636576351 1103 24-25 -0.12068041808782226 1103 26-27 -0.09803544354871008 1103 28-29 -0.16830110619758898 1103 30-31 -0.2369202166619786 1103 32-33 -0.10170420451677131 1103 34-35 -0.1636850185118348 1103 36-37 -0.12173590064360695 1103 38-39 -0.234086511991201 1103 40-41 -0.16116108124592898 1103 42-43 -0.0073536322066587445 1103 44-45 -0.1271848725924727 1103 46-47 -0.12001273000107204 1103 48-49 -0.17932663901054013 1103 50-51 -0.15507818107014515 1103 52-53 -0.1106720916124786 1103 54-55 -0.06023933992687347 1103 56-57 -0.25634775244704855 1103 58-59 -0.13382391448586617 1103 60-61 -0.20298740100929535 1103 62-63 -0.17213375630403505 1103 64-65 -0.14587623827108587 1103 66-67 -0.26188005562600125 1103 68-69 -0.16620132501228113 1103 70-71 -0.2022979946992436 1103 72-73 -0.0023571493809342314 1103 74-75 -0.1408195169501738 1103 76-77 -0.06053827882145413 1103 78-79 -0.1634489263029053 1103 80-81 -0.18992450548047657 1103 82-83 -0.07297591447284901 1103 84-85 -0.042033467082788434 1103 86-87 -0.16252415603379688 1103 88-89 0.8353232607062555 1103 90-91 -0.45663184475742646 1103 92-93 -0.31913594759125097 1103 94-95 -0.10926991482736392 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.008172737741656277 1104 2 0.034378322315635756 1104 3 0.13366004751449978 1104 4 0.22139322524417082 1104 5 0.09067791436580563 1104 6 0.12302087867811906 1104 7 0.26817920536465323 1104 8 0.1292774153837044 1104 9 0.22106302864937533 1104 10-11 0.1990471143993915 1104 12-13 0.16536388073702568 1104 14-15 0.12804389218772627 1104 16-17 0.22088028277366334 1104 18-19 0.2903418719244968 1104 20-21 0.034604485554709186 1104 22-23 -0.05541201222145986 1104 24-25 0.015167409230734563 1104 26-27 0.17867465786197556 1104 28-29 0.07249828193906183 1104 30-31 0.16537880322358234 1104 32-33 0.18184630253664835 1104 34-35 0.2174101035775564 1104 36-37 0.09792474181291055 1104 38-39 0.24265814836799393 1104 40-41 0.30798627584432126 1104 42-43 0.20681836937965414 1104 44-45 0.183770880622653 1104 46-47 0.1597929467311232 1104 48-49 0.01940940993851825 1104 50-51 0.09216560115182659 1104 52-53 -0.054795937286947094 1104 54-55 0.01036232570113782 1104 56-57 -0.03493196863876591 1104 58-59 -0.05851260067928621 1104 60-61 -0.10225089882811744 1104 62-63 -0.12416423616353 1104 64-65 -0.0851159414040552 1104 66-67 -0.016814620239905764 1104 68-69 -0.02128262352228205 1104 70-71 0.02955175885579564 1104 72-73 -0.10685774424808159 1104 74-75 -0.13449013051314296 1104 76-77 -0.037991680894990054 1104 78-79 -0.21323307450980167 1104 80-81 -0.26841807524989036 1104 82-83 -0.003939599249910941 1104 84-85 0.0167796313252353 1104 86-87 -0.03361701196264022 1104 88-89 0.27905959292539073 1104 90-91 -0.6536722390693632 1104 92-93 -0.31913594759125097 1104 94-95 -0.10926991482736392 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.07998348791763021 1105 2 0.02295738095589428 1105 3 0.23957303293788712 1105 4 0.36320027987864023 1105 5 0.2650497848453881 1105 6 0.24596068061424603 1105 7 0.24653015673885648 1105 8 0.2825877525373741 1105 9 0.5282556401993901 1105 10-11 0.4093631264167499 1105 12-13 0.5776438406791655 1105 14-15 0.5491421452198892 1105 16-17 0.5303684323230158 1105 18-19 0.6649040172455187 1105 20-21 0.5896150563792375 1105 22-23 0.3688174303066418 1105 24-25 0.3912717822139413 1105 26-27 0.40847659399194214 1105 28-29 0.4248182997425616 1105 30-31 0.47593738784581063 1105 32-33 0.29924921785877956 1105 34-35 0.5032930456049343 1105 36-37 0.2920373489120536 1105 38-39 0.3282273038147423 1105 40-41 0.46746218549159124 1105 42-43 0.5305693430082314 1105 44-45 0.3818725830817513 1105 46-47 0.5750371884135532 1105 48-49 0.5626741256389778 1105 50-51 0.4397565658786604 1105 52-53 0.5476587194394327 1105 54-55 0.27896920230066513 1105 56-57 0.4250357625284735 1105 58-59 0.35101338129468473 1105 60-61 0.31797275756444776 1105 62-63 0.3947497507063886 1105 64-65 0.45914131908332223 1105 66-67 0.7130674316127639 1105 68-69 0.9525662693229435 1105 70-71 0.748667147750858 1105 72-73 0.7812840160723837 1105 74-75 0.7263766770978535 1105 76-77 0.71491362677164 1105 78-79 0.7905924512496227 1105 80-81 0.7373547076587528 1105 82-83 0.8221180395617012 1105 84-85 0.8979057023166632 1105 86-87 0.9611721279060603 1105 88-89 1.0551884457127532 1105 90-91 -0.7989353969641 1105 92-93 -0.31913594759125097 1105 94-95 -0.10926991482736392 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.15654778566322847 1106 2 -0.0788250294560946 1106 3 -0.02194102592873648 1106 4 -0.20853502931522883 1106 5 -0.0671040014017592 1106 6 0.02668592057526098 1106 7 -0.06499457195064906 1106 8 0.038916497480208534 1106 9 0.24612902350122567 1106 10-11 0.1672967447186906 1106 12-13 0.04642910884371787 1106 14-15 0.08760465674219375 1106 16-17 0.1490713118552094 1106 18-19 0.05564008102685847 1106 20-21 0.0050935594360836944 1106 22-23 0.1144549027245958 1106 24-25 -0.04004645790327288 1106 26-27 -0.1196653388519735 1106 28-29 -0.0539955019483358 1106 30-31 0.09873659937890267 1106 32-33 0.12573424820727297 1106 34-35 -0.03815646633317016 1106 36-37 0.0993722471527434 1106 38-39 0.2530815012350658 1106 40-41 0.24060690128930418 1106 42-43 -0.21629625902276928 1106 44-45 0.020803576827262305 1106 46-47 -0.04208831730304752 1106 48-49 0.12081091056835191 1106 50-51 -0.1167395962850506 1106 52-53 -0.1362587775301165 1106 54-55 0.16654776849647135 1106 56-57 -0.21051058740211204 1106 58-59 0.12748444187507602 1106 60-61 0.18303854747233572 1106 62-63 0.18935841020265087 1106 64-65 0.22977972648575928 1106 66-67 0.22000831639407892 1106 68-69 0.23187253283896325 1106 70-71 0.21617643709665657 1106 72-73 0.06184590874496365 1106 74-75 0.09972422952848348 1106 76-77 -0.0025423134597559738 1106 78-79 0.07063570253165352 1106 80-81 0.04295768464933758 1106 82-83 0.01610311940937237 1106 84-85 0.03394979398474618 1106 86-87 -0.07036929850626095 1106 88-89 0.6297628077783433 1106 90-91 -0.23441926793184198 1106 92-93 -0.25982273219062657 1106 94-95 -0.10926991482736392 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.2970190960616961 1107 2 -0.36355894509531694 1107 3 -0.23597312632920264 1107 4 -0.3023388321720972 1107 5 -0.3038250096108044 1107 6 -0.4093129862873255 1107 7 -0.2895985724131265 1107 8 -0.3356880285291055 1107 9 -0.3163532392932069 1107 10-11 -0.40760891962506207 1107 12-13 -0.339351696083817 1107 14-15 -0.3089151721397556 1107 16-17 -0.48948122706153185 1107 18-19 -0.4049691328601881 1107 20-21 -0.3360015750513483 1107 22-23 -0.18845294789397116 1107 24-25 -0.15801175079052854 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0.0608630394856533 2216 5 -0.06187239523171684 2216 6 0.1075003678422064 2216 7 0.13082626418817966 2216 8 0.05244885501218732 2216 9 -0.05682783822368975 2216 10-11 -0.0673441394705776 2216 12-13 -0.09114443969640718 2216 14-15 -0.09956059697636022 2216 16-17 -0.24558546800032843 2216 18-19 -0.14470843767302455 2216 20-21 -0.09534036738646279 2216 22-23 -0.11545458188090407 2216 24-25 -0.06908665423674876 2216 26-27 -0.060567795698396765 2216 28-29 -0.06368592858725464 2216 30-31 -0.16913454816960183 2216 32-33 -0.15149831820638582 2216 34-35 -0.23306009766083235 2216 36-37 -0.13093845478151422 2216 38-39 -0.3018919677310805 2216 40-41 -0.2908788557346256 2216 42-43 -0.30376115848412155 2216 44-45 -0.25965307448571195 2216 46-47 -0.4002496229283139 2216 48-49 -0.33723629439583647 2216 50-51 -0.36171888027231347 2216 52-53 -0.5578406354293648 2216 54-55 -0.39200222538245555 2216 56-57 -0.40094009557374477 2216 58-59 -0.43484483752138914 2216 60-61 -0.44884048861139547 2216 62-63 -0.40155007207684434 2216 64-65 -0.4517742231377966 2216 66-67 -0.4321735598683958 2216 68-69 -0.43764872259348664 2216 70-71 -0.4001531560048903 2216 72-73 -0.473156118861084 2216 74-75 -0.5911629246307939 2216 76-77 -0.5380565028764153 2216 78-79 -0.3169588872032989 2216 80-81 -0.4719165272622803 2216 82-83 -0.4552660311384571 2216 84-85 -0.5758780699131556 2216 86-87 -0.6100721203217709 2216 88-89 -1.2602486501086627 2216 90-91 -0.7989353969641 2216 92-93 -0.31913594759125097 2216 94-95 -0.10926991482736392 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7573.0 3 1280.0 4 883.0 5 848.0 6 796.0 7 903.0 8 940.0 9 1091.0 10 1377.0 11 1735.0 12 2138.0 13 2553.0 14 2886.0 15 3376.0 16 3782.0 17 4446.0 18 5610.0 19 7098.0 20 6705.0 21 9729.0 22 10845.0 23 12874.0 24 19821.0 25 33862.0 26 59540.0 27 162220.0 28 259030.0 29 9679.0 30 435.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.997232101316136 20.860335459857584 15.417432241682505 22.72500019714378 2 12.804175058473213 20.50686774407737 45.84284229502043 20.846114902428983 3 19.030653783284002 27.026860716832758 29.384589168617897 24.557896331265344 4 11.19812417849969 18.072138969844527 40.1269272219759 30.60280962967989 5 11.602920454384074 39.67828925879348 34.571438940759336 14.147351346063115 6 27.988108287135976 37.46725441799213 21.12845100188469 13.4161862929872 7 27.485470503347504 32.49134538801839 22.551198239900323 17.471985868733785 8 24.081980269850405 39.127993628313 20.944555283059042 15.845470818777551 9 25.612920014825214 19.08998430735504 22.56381544187807 32.73328023594168 10-11 23.502525822055322 27.249779591862485 30.506536540436024 18.74115804564617 12-13 26.183668061938725 25.195332830740984 29.471363276481515 19.149635830838772 14-15 20.322684940580864 30.676834028593735 26.24338582615073 22.757095204674673 16-17 19.318322507485565 31.274332528721942 30.498609344479604 18.908735619312882 18-19 20.71130416541473 28.951599992744693 31.389620334422418 18.94747550741816 20-21 21.932783844654313 29.160988827273815 28.63933733305575 20.26688999501612 22-23 20.380521140632897 31.45375415088522 29.081312795569445 19.084411912912437 24-25 21.061658688915 29.342722634471773 30.88643729644905 18.709181380164182 26-27 18.9520625182358 31.22079314885933 30.200298081396724 19.62684625150815 28-29 19.00585048959111 31.44573097210864 28.128560940431306 21.419857597868937 30-31 20.198405501100062 29.49783536128569 29.807351097302288 20.496408040311962 32-33 19.01550118011477 30.760186326440326 30.947632554922848 19.276679938522054 34-35 19.448959040587205 31.10702628874963 30.526792255239886 18.91722241542328 36-37 18.847118031651853 30.405430448013536 30.09914762847673 20.64830389185788 38-39 20.25770635039547 31.152660258179495 28.98912554904543 19.600507842379606 40-41 22.07403143260443 28.583719077997966 30.63772070246272 18.704528786934887 42-43 19.093611752923643 31.393806530979173 30.499325768269316 19.01325594782787 44-45 18.796269718352065 31.506070460875574 29.440473524337047 20.25718629643532 46-47 20.891326462215424 29.474651252651583 29.34587693496621 20.288145350166783 48-49 19.894204594561344 30.190646222561483 29.266119289524234 20.64902989335293 50-51 18.567173642013422 31.696959803928525 29.6689795593281 20.06688699472995 52-53 18.704923074496694 30.621002909842204 30.432848885349063 20.241225130312042 54-55 19.130822217578334 29.68192446149539 30.56766251739814 20.619590803528126 56-57 19.888416620009306 30.176561970176092 28.14203815126448 21.792983258550123 58-59 20.190756322401054 30.870034933877978 27.78244789489871 21.156760848822262 60-61 19.35644384162257 28.86508268210171 31.28316944113681 20.495304035138908 62-63 19.98533250270087 28.854752347982433 30.678726608890393 20.481188540426302 64-65 20.46447074780579 30.208972407756423 28.645701082713643 20.68085576172414 66-67 20.327258676297795 31.664682085938917 27.73678939524174 20.271269842521548 68-69 21.13349788267579 29.083517991341445 30.323079228142667 19.459904897840094 70-71 22.005819684412238 28.932663570195018 29.335862030896376 19.725654714496375 72-73 20.65081104951463 30.62289549013887 28.77684112576985 19.94945233457665 74-75 19.85955477048521 31.136573325657867 30.119469131226783 18.884402772630136 76-77 20.258968070593244 29.356522699134935 30.706563310753797 19.677945919518024 78-79 19.876272563105722 29.355970696548404 29.96230610909148 20.805450631254384 80-81 20.49972005583112 30.29587338637815 29.52866864861881 19.675737909171918 82-83 19.916836408399003 30.037149979142075 30.92721963436724 19.118793978091688 84-85 19.697818012632972 29.888337762496942 30.779427652175283 19.6344165726948 86-87 18.628952382154033 31.893484404966188 28.546340340163525 20.931222872716262 88-89 29.104693569932934 18.971489371238377 35.86448268033623 16.05933437849246 90-91 31.049835194568796 16.13906113308279 18.00400409821163 34.80709957413678 92-93 22.514443607133884 18.26412300532794 37.065006147622256 22.156427239915917 94-95 16.650875585754118 7.539968056899182 29.33422428274061 46.47493207460609 96-97 28.26293471905267 7.595315523811425 16.24112375863069 47.90062599850521 98-99 57.23577421641023 14.130906929425482 18.818728424132964 9.814590430031322 100-101 30.951084103317932 7.430734197816044 13.18527535699286 48.432906341873164 102-103 23.843836659601926 2.264311154091091 38.18177836032873 35.710073825978256 104-105 41.43050736867201 4.846258571688489 42.4261721176227 11.297061942016802 106-107 6.809374187304687 2.2838603507006767 7.728545909557936 83.1782195524367 108-109 18.79372096170328 12.11980693761559 23.69191212955027 45.39455997113086 110-111 41.03129052088876 2.91569588733966 26.709690917638305 29.343322674133272 112-113 27.990181138230135 2.9161689964497235 40.190775871300424 28.902873994019718 114-115 30.598266205272733 4.386215261674574 25.153698881000796 39.86181965205189 116-117 21.324545326378118 4.558014944311293 21.761595939658818 52.35584378965177 118-119 21.96051156122695 4.542447942541624 17.102099331450226 56.3949411647812 120-121 26.751508000529757 4.576776591176341 30.943408342062277 37.72830706623162 122-123 35.109329374564695 1.8406312230436959 34.84937168717444 28.200667715217172 124-125 39.95531041657416 2.3110762926358923 20.868037274093997 36.86557601669595 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 24.0 1 54.5 2 99.0 3 142.5 4 183.5 5 176.0 6 138.0 7 103.0 8 82.0 9 74.0 10 70.0 11 73.5 12 74.5 13 74.0 14 83.0 15 120.0 16 167.0 17 210.5 18 288.5 19 444.0 20 657.0 21 930.0 22 1340.0 23 1923.0 24 2625.5 25 3502.0 26 4733.0 27 6204.5 28 8113.5 29 10685.5 30 13873.0 31 17810.0 32 22677.0 33 28041.5 34 32958.5 35 36452.0 36 37491.0 37 36561.0 38 34458.0 39 31856.5 40 29436.5 41 26938.0 42 24491.5 43 22477.0 44 20888.5 45 19513.0 46 18225.5 47 16968.5 48 15801.5 49 14890.5 50 13881.0 51 12541.0 52 11038.0 53 9545.5 54 8204.5 55 6866.0 56 5620.5 57 4629.0 58 3774.0 59 2998.0 60 2300.0 61 1772.0 62 1401.5 63 1071.0 64 805.5 65 584.0 66 431.5 67 338.0 68 253.0 69 192.0 70 156.5 71 114.5 72 76.0 73 58.0 74 46.0 75 40.5 76 34.5 77 23.5 78 13.0 79 7.5 80 5.0 81 5.0 82 5.0 83 5.5 84 4.0 85 1.5 86 1.0 87 2.0 88 1.5 89 1.0 90 2.0 91 2.5 92 1.5 93 0.0 94 0.5 95 1.0 96 1.0 97 2.0 98 3.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 6.308600988873205E-4 3 0.04210991160072864 4 0.04826079756488002 5 0.05015337786154198 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.308600988873205E-4 12-13 0.0014194352224964713 14-15 0.0 16-17 2.3657253708274522E-4 18-19 0.0057565984023468 20-21 0.0017348652719401316 22-23 5.520025865264055E-4 24-25 0.0 26-27 0.0 28-29 8.674326359700658E-4 30-31 0.0 32-33 0.0010251476606918958 34-35 7.885751236091507E-5 36-37 7.885751236091507E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.5771502472183014E-4 46-47 0.0 48-49 1.5771502472183014E-4 50-51 7.885751236091507E-5 52-53 0.0 54-55 0.001182862685413726 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.520025865264055E-4 84-85 0.0 86-87 0.001104005173052811 88-89 19.067036771258014 90-91 26.26491392702526 92-93 40.35296622532746 94-95 44.65204122670746 96-97 48.616523803140105 98-99 93.10138710364242 100-101 51.933507345577276 102-103 53.19349267807998 104-105 74.86164449456277 106-107 81.50326075813612 108-109 12.590390423543699 110-111 38.94125903904235 112-113 52.13388428448636 114-115 86.73727042606714 116-117 66.43981988944176 118-119 72.22204698330586 120-121 80.35099478751843 122-123 75.88387442729731 124-125 50.27466071555307 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 634055.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.54693651208487 #Duplication Level Percentage of deduplicated Percentage of total 1 90.57992553819038 72.05355586055803 2 6.729074788776606 10.705545700157671 3 1.2851037655530733 3.0667820294967454 4 0.4610372910130031 1.466964164716598 5 0.23384204088045202 0.9300708989886836 6 0.1578292716387276 0.7532901030476676 7 0.10339478862417315 0.5757317080457274 8 0.07141882752984076 0.4544919151427025 9 0.05313151995048958 0.38038046798629477 >10 0.28127140907835774 4.137196730690959 >50 0.025154546566513107 1.3767256990811205 >100 0.01703362367452923 2.7885650825444483 >500 0.0011883923492448174 0.6848913731331961 >1k 5.941961746224087E-4 0.6258082664101806 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1414 0.2230090449566678 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1306 0.20597582228671016 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1252 0.1974592109517313 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 866 0.13658121140910487 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 851 0.13421548603827743 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 800 0.12617201977746412 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 731 0.11528968307165782 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.5771502472183014E-4 14-15 0.0 0.0 0.0 1.5771502472183014E-4 1.5771502472183014E-4 16-17 0.0 0.0 0.0 1.5771502472183014E-4 2.365725370827452E-4 18-19 0.0 0.0 0.0 1.5771502472183014E-4 3.154300494436603E-4 20-21 0.0 0.0 0.0 1.5771502472183014E-4 3.942875618045753E-4 22-23 0.0 0.0 0.0 1.5771502472183014E-4 4.731450741654904E-4 24-25 0.0 0.0 0.0 3.942875618045753E-4 4.731450741654904E-4 26-27 0.0 0.0 0.0 8.674326359700657E-4 4.731450741654904E-4 28-29 0.0 0.0 0.0 0.0012617201977746409 4.731450741654904E-4 30-31 0.0 0.0 0.0 0.0037063030809630078 4.731450741654904E-4 32-33 0.0 0.0 0.0 0.008043466260813337 4.731450741654904E-4 34-35 0.0 0.0 0.0 0.012696059490107325 4.731450741654904E-4 36-37 0.0 0.0 0.0 0.020108665652033342 4.731450741654904E-4 38-39 0.0 0.0 0.0 0.03540702305005086 4.731450741654904E-4 40-41 0.0 0.0 0.0 0.058512274171798974 4.731450741654904E-4 42-43 0.0 0.0 0.0 0.0790940848979978 4.731450741654904E-4 44-45 0.0 0.0 0.0 0.09983361064891846 4.731450741654904E-4 46-47 0.0 0.0 0.0 0.12806460007412607 4.731450741654904E-4 48-49 0.0 0.0 0.0 0.1601596076050185 4.731450741654904E-4 50-51 0.0 0.0 0.0 0.1989575036865887 4.731450741654904E-4 52-53 0.0 0.0 0.0 0.23302394902650403 4.731450741654904E-4 54-55 0.0 0.0 0.0 0.2733989953552925 4.731450741654904E-4 56-57 0.0 0.0 0.0 0.32300037063030806 4.731450741654904E-4 58-59 0.0 0.0 0.0 0.38640181056848383 4.731450741654904E-4 60-61 0.0 0.0 0.0 0.45926615198996934 4.731450741654904E-4 62-63 0.0 0.0 0.0 0.5296070530159056 4.731450741654904E-4 64-65 0.0 0.0 0.0 0.5953742183249087 4.731450741654904E-4 66-67 0.0 0.0 0.0 0.6780168912791478 4.731450741654904E-4 68-69 0.0 0.0 0.0 0.7855785381394358 4.731450741654904E-4 70-71 0.0 0.0 0.0 0.8944019051974987 4.731450741654904E-4 72-73 0.0 0.0 0.0 1.010953308466931 5.520025865264055E-4 74-75 0.0 0.0 0.0 1.12718928168692 6.308600988873205E-4 76-77 0.0 0.0 0.0 1.2509955760935565 6.308600988873205E-4 78-79 0.0 0.0 0.0 1.2885317519773523 6.308600988873205E-4 80-81 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 82-83 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 84-85 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 86-87 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 88-89 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 90-91 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 92-93 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 94-95 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 96-97 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 98-99 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 100-101 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 102-103 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 104-105 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 106-107 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 108-109 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 110-111 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 112-113 0.0 0.0 0.0 1.2890048970515176 6.308600988873205E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGCA 5 3.135433E-4 5099.1313 108-109 CTCCGCC 65 0.0 2695.0745 110-111 CCGCTCC 190 0.0 2683.7534 108-109 CCGCTCG 40 1.8189894E-12 2549.5657 108-109 CGTCGCG 10 0.0012540108 2549.5657 108-109 CCGCGCA 10 0.0012540108 2549.5657 108-109 CGTCGTG 15 0.0021499004 1946.4426 110-111 CAGCGCG 40 4.0163286E-9 1912.1743 108-109 CAACGCG 15 0.0028211589 1699.7106 108-109 TAGTTCG 15 0.0028211589 1699.7106 108-109 GGACGCC 15 0.0028211589 1699.7106 108-109 CCGCGCG 60 9.094947E-12 1699.7106 108-109 CCGCGCC 45 6.4319465E-9 1699.7104 108-109 CGTGTTG 35 4.624382E-6 1668.3795 110-111 TTGTGCG 40 6.901995E-6 1459.832 110-111 CGTAGTT 20 0.0038216135 1459.832 110-111 CGTCGTT 20 0.0038216135 1459.832 110-111 CGTCGCT 20 0.0038216135 1459.832 110-111 CTGCGCG 35 6.9581292E-6 1456.8948 108-109 CTCCGCT 45 9.826013E-6 1297.6284 110-111 >>END_MODULE