##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939900_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 634055 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.822386070609014 34.0 31.0 34.0 31.0 34.0 2 32.92315808565503 34.0 33.0 34.0 31.0 34.0 3 32.959203854555206 34.0 33.0 34.0 31.0 34.0 4 36.34986554794143 37.0 37.0 37.0 35.0 37.0 5 36.265976926291884 37.0 37.0 37.0 35.0 37.0 6 36.41031929406755 37.0 37.0 37.0 35.0 37.0 7 36.40766810450197 37.0 37.0 37.0 35.0 37.0 8 36.43616247801847 37.0 37.0 37.0 35.0 37.0 9 38.26929998186277 39.0 39.0 39.0 37.0 39.0 10-11 38.263033175355446 39.0 39.0 39.0 37.0 39.0 12-13 38.252498600279154 39.0 39.0 39.0 37.0 39.0 14-15 39.76693267934169 41.0 40.0 41.0 37.5 41.0 16-17 39.780058512274174 41.0 40.0 41.0 37.5 41.0 18-19 39.84994992547965 41.0 40.0 41.0 38.0 41.0 20-21 39.87799244545032 41.0 40.0 41.0 38.0 41.0 22-23 39.82912050216464 41.0 40.0 41.0 37.5 41.0 24-25 39.79780776115636 41.0 40.0 41.0 37.0 41.0 26-27 39.70050232235374 41.0 40.0 41.0 37.0 41.0 28-29 39.61703243409484 41.0 40.0 41.0 37.0 41.0 30-31 39.461707580572664 41.0 39.0 41.0 36.5 41.0 32-33 39.28577568192034 41.0 39.0 41.0 35.0 41.0 34-35 39.18010188390597 41.0 39.0 41.0 35.0 41.0 36-37 39.15052006529402 41.0 39.0 41.0 35.0 41.0 38-39 39.1762583687535 41.0 39.0 41.0 35.0 41.0 40-41 39.05758333267619 41.0 39.0 41.0 35.0 41.0 42-43 38.94997910275923 41.0 39.0 41.0 35.0 41.0 44-45 38.76151989969324 41.0 39.0 41.0 35.0 41.0 46-47 38.44588008926671 41.0 39.0 41.0 35.0 41.0 48-49 38.13365402055027 41.0 39.0 41.0 35.0 41.0 50-51 37.95503702360205 41.0 39.0 41.0 34.0 41.0 52-53 37.85536901372909 41.0 39.0 41.0 34.0 41.0 54-55 37.75698953560811 41.0 39.0 41.0 34.0 41.0 56-57 37.61660108350222 41.0 38.5 41.0 33.5 41.0 58-59 37.4754934508836 40.5 38.0 41.0 33.0 41.0 60-61 37.31248156705648 40.0 38.0 41.0 33.0 41.0 62-63 37.123908809172704 40.0 37.0 41.0 33.0 41.0 64-65 36.89472837529867 40.0 37.0 41.0 32.5 41.0 66-67 36.63446625292758 39.0 36.5 41.0 32.0 41.0 68-69 36.307988266002155 39.0 36.0 41.0 31.5 41.0 70-71 35.94041368650984 39.0 35.0 41.0 31.0 41.0 72-73 35.60802927190859 37.5 35.0 40.0 31.0 41.0 74-75 35.259363935305295 37.0 35.0 39.5 31.0 41.0 76-77 34.867942844075046 36.5 35.0 39.0 30.5 41.0 78-79 34.494957850659645 36.0 35.0 39.0 30.0 40.5 80-81 34.19445395115565 36.0 35.0 37.5 30.0 39.0 82-83 33.93518227913982 35.0 35.0 37.0 30.0 39.0 84-85 33.629794733895324 35.0 35.0 37.0 30.0 39.0 86-87 33.38731892343724 35.0 35.0 36.0 30.0 37.5 88-89 33.139446893408305 35.0 35.0 36.0 30.0 37.0 90-91 32.929379943380304 35.0 34.5 36.0 29.5 37.0 92-93 32.78156390218514 35.0 34.0 35.5 29.0 36.5 94-95 32.66958544605752 35.0 34.0 35.0 29.0 36.0 96-97 32.532658838744275 35.0 34.0 35.0 29.0 36.0 98-99 32.40455559848909 35.0 34.0 35.0 29.0 36.0 100-101 32.39705703763869 35.0 34.0 35.0 29.0 36.0 102-103 31.577863119130043 34.5 32.5 35.0 26.0 35.0 104-105 32.2271719330342 35.0 34.0 35.0 29.0 35.0 106-107 32.285972825701236 35.0 34.0 35.0 29.0 35.0 108-109 32.292484879072006 35.0 34.0 35.0 29.0 35.0 110-111 32.28043308545789 35.0 34.0 35.0 29.0 35.0 112-113 32.199229562104236 35.0 34.0 35.0 29.0 35.0 114-115 32.13193334963056 35.0 34.0 35.0 29.0 35.0 116-117 32.07367893952417 35.0 34.0 35.0 29.0 35.0 118-119 31.9988810118996 35.0 34.0 35.0 29.0 35.0 120-121 31.9019272776021 35.0 34.0 35.0 27.0 35.0 122-123 31.736129357863277 35.0 34.0 35.0 27.0 35.0 124-125 29.719796389903085 33.5 29.5 34.5 13.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13866953426390438 1101 2 0.14187757388453548 1101 3 0.17772421346067802 1101 4 0.08567373699867176 1101 5 -0.03174546414644652 1101 6 -0.004561669706056648 1101 7 -0.018914960640231016 1101 8 0.0790248869741248 1101 9 0.08060252558199466 1101 10-11 0.09020011036995612 1101 12-13 0.0287882052960029 1101 14-15 0.10523781871292215 1101 16-17 0.16179115088667118 1101 18-19 0.16500797353826613 1101 20-21 0.1526961265539697 1101 22-23 0.21614889453260844 1101 24-25 0.18151318789242765 1101 26-27 0.21796998720797234 1101 28-29 0.19474422789052426 1101 30-31 0.3315294884850317 1101 32-33 0.3634540598890865 1101 34-35 0.40699385710725267 1101 36-37 0.5071629344228725 1101 38-39 0.39898416608908605 1101 40-41 0.5278340310838914 1101 42-43 0.5136499162394728 1101 44-45 0.5393306205442627 1101 46-47 0.6975301593393226 1101 48-49 0.7449915169332186 1101 50-51 0.7132637327901747 1101 52-53 0.7023273441979612 1101 54-55 0.7983877084507824 1101 56-57 0.7481718824807544 1101 58-59 0.70942639893088 1101 60-61 0.7478005015807696 1101 62-63 0.8300879622984283 1101 64-65 0.7910305666991349 1101 66-67 0.7250737864292773 1101 68-69 0.6834028265191918 1101 70-71 0.7502194180691504 1101 72-73 0.6859598583657274 1101 74-75 0.6463434942669863 1101 76-77 0.6772490387607988 1101 78-79 0.6492528609120498 1101 80-81 0.667313708576728 1101 82-83 0.6581412235185482 1101 84-85 0.6208623828991051 1101 86-87 0.6559085487558036 1101 88-89 0.5890741197407507 1101 90-91 0.6285072334757587 1101 92-93 0.5777420515675331 1101 94-95 0.6434261489869186 1101 96-97 0.6622161591441511 1101 98-99 0.7090522188502106 1101 100-101 0.5305847853936356 1101 102-103 0.6363379108497149 1101 104-105 0.6677006519899464 1101 106-107 0.7692531415885924 1101 108-109 0.7715854531867521 1101 110-111 0.7526542116972905 1101 112-113 0.6540773678961713 1101 114-115 0.5501627212275437 1101 116-117 0.7562236883899587 1101 118-119 0.7621826561705376 1101 120-121 0.7059201533903057 1101 122-123 0.6691892257517473 1101 124-125 0.9122712094372538 1102 1 0.014237541287315025 1102 2 0.05708599613520704 1102 3 0.09342023713792003 1102 4 -0.02805412790290518 1102 5 0.015415686301089693 1102 6 -0.02309781969760394 1102 7 0.029415946845610108 1102 8 0.011327326318820496 1102 9 0.06850125526476347 1102 10-11 0.15505778790369362 1102 12-13 0.025635819514512548 1102 14-15 0.04453204908696762 1102 16-17 0.12163577505054235 1102 18-19 0.040099930789843086 1102 20-21 -0.006712453042403865 1102 22-23 0.060571208438489066 1102 24-25 0.16623156462957667 1102 26-27 0.07174245365649767 1102 28-29 0.1022033137533711 1102 30-31 0.14543111459322233 1102 32-33 0.15811488506634674 1102 34-35 0.23500309356718674 1102 36-37 0.2934646174381683 1102 38-39 0.31295048146385085 1102 40-41 0.37932838962050397 1102 42-43 0.14418604677560154 1102 44-45 0.18852936129231068 1102 46-47 0.22114356558184056 1102 48-49 0.3247611647779891 1102 50-51 0.3761688158830552 1102 52-53 0.5664397576061404 1102 54-55 0.44297369106492823 1102 56-57 0.23941512020287092 1102 58-59 0.3827571544212631 1102 60-61 0.3993703714604919 1102 62-63 0.30796471951790494 1102 64-65 0.18895771345256662 1102 66-67 0.3454389889541005 1102 68-69 0.237440859430464 1102 70-71 0.08636892323274736 1102 72-73 0.10444773460008605 1102 74-75 0.07459981454208275 1102 76-77 0.13515845676411686 1102 78-79 0.17592198382530455 1102 80-81 0.16966051843527197 1102 82-83 0.10124324408535301 1102 84-85 0.12646128755903874 1102 86-87 0.11478465786665737 1102 88-89 0.1733993754275076 1102 90-91 0.17788812557965628 1102 92-93 0.2337435009962121 1102 94-95 0.21133224740944456 1102 96-97 0.14528933705301483 1102 98-99 0.21891793998353393 1102 100-101 0.17840948720894545 1102 102-103 0.17713774742420085 1102 104-105 0.16576605381121823 1102 106-107 0.18220047941856876 1102 108-109 0.1508299263279369 1102 110-111 0.022073455294943756 1102 112-113 0.05553263320120294 1102 114-115 0.11447321511550257 1102 116-117 0.15801273571890562 1102 118-119 -0.0230039881710411 1102 120-121 0.0913642172381266 1102 122-123 0.05405327493504686 1102 124-125 0.09397022681702083 1103 1 -0.014866785759672041 1103 2 0.007661353554212269 1103 3 0.09729910361325267 1103 4 0.06781545075143924 1103 5 -0.07574000832039474 1103 6 0.06156666301718161 1103 7 -0.03152400769491237 1103 8 0.03534807580538768 1103 9 0.05879009436877425 1103 10-11 0.02311918268623714 1103 12-13 0.10610363926733868 1103 14-15 0.06240970502008025 1103 16-17 0.07536052480767808 1103 18-19 0.06550499010992183 1103 20-21 0.04188825112463945 1103 22-23 -0.022707798217169284 1103 24-25 0.09428943741068707 1103 26-27 0.05543686988476537 1103 28-29 0.01189371583298282 1103 30-31 -0.09645807764390923 1103 32-33 -0.1462023656266851 1103 34-35 -0.024397559752415532 1103 36-37 -0.006536432164907069 1103 38-39 0.06641737873220421 1103 40-41 0.08203589906401021 1103 42-43 -0.02448166871665336 1103 44-45 -0.35702982640820835 1103 46-47 -0.23959253271698344 1103 48-49 -0.2810499043360508 1103 50-51 -0.26649498342350597 1103 52-53 -0.19474347160267058 1103 54-55 -0.22844864938665665 1103 56-57 -0.22680573727619446 1103 58-59 -0.08620724887355635 1103 60-61 -0.1946052567560983 1103 62-63 -0.2793021957103221 1103 64-65 -0.27503856862009 1103 66-67 -0.12911455071564149 1103 68-69 -0.14011944318176717 1103 70-71 -0.07324305017264976 1103 72-73 -0.0768548694651443 1103 74-75 -0.1462203453008044 1103 76-77 -0.22608401670716916 1103 78-79 -0.20629097597373658 1103 80-81 -0.3790294019931508 1103 82-83 -0.32364832608585914 1103 84-85 -0.29290995354993754 1103 86-87 -0.29653962633319964 1103 88-89 -0.225355911947986 1103 90-91 -0.17319535635025574 1103 92-93 -0.17809100692070956 1103 94-95 -0.21520078534867082 1103 96-97 -0.22935715080016905 1103 98-99 -0.20540451207584454 1103 100-101 -0.20364544901600823 1103 102-103 -0.20502331533390006 1103 104-105 -0.3072002527638489 1103 106-107 -0.29877313239390446 1103 108-109 -0.39238048293562144 1103 110-111 -0.3183693839047024 1103 112-113 -0.26584183368790093 1103 114-115 -0.2553194416281457 1103 116-117 -0.24662382755407464 1103 118-119 -0.24897303421012396 1103 120-121 -0.24987690341141544 1103 122-123 -0.2003034524958629 1103 124-125 -0.11797262346176396 1104 1 0.13984739474001628 1104 2 0.15853892423009341 1104 3 0.11048929113803752 1104 4 0.06382247584916456 1104 5 0.011651074337812872 1104 6 0.05210204344888325 1104 7 -0.022962453108775094 1104 8 -0.02981083870370327 1104 9 -0.05896980592015666 1104 10-11 0.09498479936517157 1104 12-13 0.15736815685841066 1104 14-15 0.08241367080105277 1104 16-17 0.07984242385022355 1104 18-19 0.15189556288199668 1104 20-21 0.18847201408424752 1104 22-23 0.08883659020275303 1104 24-25 0.06964834058897651 1104 26-27 0.1313050926483399 1104 28-29 0.159793551536346 1104 30-31 0.1830966218065484 1104 32-33 0.22737986767454288 1104 34-35 0.2142931659855094 1104 36-37 0.22856526179011638 1104 38-39 0.13175928570630902 1104 40-41 0.16240075091376838 1104 42-43 0.3477487998120381 1104 44-45 0.37409758846912666 1104 46-47 0.25629381933104867 1104 48-49 0.33729466537671726 1104 50-51 0.31547827588191524 1104 52-53 0.4218329263351208 1104 54-55 0.45073043395139933 1104 56-57 0.5793769152647386 1104 58-59 0.5598564302381064 1104 60-61 0.5989860411873593 1104 62-63 0.4894706783478071 1104 64-65 0.4279193374475554 1104 66-67 0.4739957546494935 1104 68-69 0.39834523304055125 1104 70-71 0.4566238128945983 1104 72-73 0.468199801658308 1104 74-75 0.4765591006229357 1104 76-77 0.5036593406097012 1104 78-79 0.46819432230964964 1104 80-81 0.5006624001710378 1104 82-83 0.5054440766108712 1104 84-85 0.4399544733947067 1104 86-87 0.4463202682903358 1104 88-89 0.48840308187672576 1104 90-91 0.5653044453606881 1104 92-93 0.5154022795786375 1104 94-95 0.5069939911512478 1104 96-97 0.43506866076858586 1104 98-99 0.5103600338422325 1104 100-101 0.4642719494316765 1104 102-103 0.4777963549397377 1104 104-105 0.534465375245901 1104 106-107 0.5625055487006563 1104 108-109 0.6319971727212845 1104 110-111 0.5063142034274648 1104 112-113 0.6186842554855119 1104 114-115 0.5998224766767706 1104 116-117 0.6329252064949813 1104 118-119 0.7236659097590561 1104 120-121 0.6123552647376975 1104 122-123 0.632381381224608 1104 124-125 0.499204873563901 1105 1 0.021307281242741283 1105 2 0.09654048203581311 1105 3 0.0928705084519379 1105 4 0.02012157454728225 1105 5 -0.09500075608390546 1105 6 0.019190059472030896 1105 7 0.01882679803176046 1105 8 0.02581840353508369 1105 9 -0.013686619094741559 1105 10-11 0.10764197083507554 1105 12-13 0.10048710645227033 1105 14-15 0.12037628346379137 1105 16-17 0.059750068225433495 1105 18-19 0.021923380841272433 1105 20-21 0.17086880945533522 1105 22-23 0.16197668144566535 1105 24-25 0.08867916288785693 1105 26-27 0.24001211390126542 1105 28-29 0.25880211946779497 1105 30-31 0.1118781951903216 1105 32-33 0.22694034391800955 1105 34-35 0.26722406617466987 1105 36-37 0.3670864590750824 1105 38-39 0.33185943035969245 1105 40-41 0.333565767048178 1105 42-43 0.2983574567609679 1105 44-45 0.34568877573963164 1105 46-47 0.6186861638286558 1105 48-49 0.5771633646089427 1105 50-51 0.6998479209247535 1105 52-53 0.6714957726687132 1105 54-55 0.7132729954290937 1105 56-57 0.7419272657710252 1105 58-59 0.5843317835261885 1105 60-61 0.6768585236620481 1105 62-63 0.7938469997008681 1105 64-65 0.8024692428664935 1105 66-67 0.6250750914693413 1105 68-69 0.655546746337528 1105 70-71 0.6571178598513185 1105 72-73 0.5981241368090835 1105 74-75 0.61560327554227 1105 76-77 0.6934445859641016 1105 78-79 0.6012665377319237 1105 80-81 0.47139456885913944 1105 82-83 0.6209475816737395 1105 84-85 0.5438845946810105 1105 86-87 0.5323734562284699 1105 88-89 0.6116121616586341 1105 90-91 0.5103228052138107 1105 92-93 0.4505919980608937 1105 94-95 0.5780110157423195 1105 96-97 0.702887732761468 1105 98-99 0.5971776593012308 1105 100-101 0.5001382006834802 1105 102-103 0.4986431336200532 1105 104-105 0.5847457802753837 1105 106-107 0.6622985830836541 1105 108-109 0.5973104029427105 1105 110-111 0.6073902020922048 1105 112-113 0.5687799492654193 1105 114-115 0.6859390895620479 1105 116-117 0.5735572305297083 1105 118-119 0.7653539035278314 1105 120-121 0.6129817251805569 1105 122-123 0.531970788145717 1105 124-125 0.46842708342258277 1106 1 -0.0314511429674198 1106 2 0.04515593501929516 1106 3 0.036696640938131964 1106 4 -0.0054409011156195675 1106 5 -0.02481642114386773 1106 6 0.03219624356937345 1106 7 -0.09914844357922448 1106 8 -0.07088209123670453 1106 9 -0.09258500938146597 1106 10-11 0.016886327378756505 1106 12-13 -0.028530851849623673 1106 14-15 -0.16876711675576672 1106 16-17 -0.0805163033528018 1106 18-19 -0.05323177158319936 1106 20-21 -0.07526915465445683 1106 22-23 -0.015376674488628339 1106 24-25 0.04713670249270052 1106 26-27 0.046834420650974096 1106 28-29 0.026915518785365578 1106 30-31 0.018970437459103096 1106 32-33 0.13244586252639579 1106 34-35 0.22175962088445544 1106 36-37 0.24964965852596777 1106 38-39 0.21234146435793377 1106 40-41 0.2461304184749693 1106 42-43 0.2769804116199168 1106 44-45 0.14285984269992014 1106 46-47 0.19410476187992742 1106 48-49 0.45043886823558665 1106 50-51 0.3705639870380608 1106 52-53 0.4533127445069809 1106 54-55 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-0.3497126019869796 2216 38-39 -0.3987263954082394 2216 40-41 -0.3654601236992434 2216 42-43 -0.37928680390312763 2216 44-45 -0.4669608480633798 2216 46-47 -0.4246794778825702 2216 48-49 -0.37327035010316223 2216 50-51 -0.5341743943967217 2216 52-53 -0.5894228786184428 2216 54-55 -0.5904934437575733 2216 56-57 -0.6546817147662196 2216 58-59 -0.6729580047464125 2216 60-61 -0.6786097405463849 2216 62-63 -0.7358785863580763 2216 64-65 -0.7271222647196254 2216 66-67 -0.8081975503040724 2216 68-69 -0.855364132284528 2216 70-71 -0.7860454982509069 2216 72-73 -0.8664422262054714 2216 74-75 -0.9786198854451698 2216 76-77 -0.9853305974707922 2216 78-79 -0.7593588897646555 2216 80-81 -0.7980837298599255 2216 82-83 -0.6774032531104908 2216 84-85 -0.6983427402275879 2216 86-87 -0.6336594143722039 2216 88-89 -0.5195700218384403 2216 90-91 -0.4797913363730544 2216 92-93 -0.514043347356349 2216 94-95 -0.6286058540499937 2216 96-97 -0.5925609831942609 2216 98-99 -0.5132767695633405 2216 100-101 -0.6656148539738993 2216 102-103 -0.7153344500138239 2216 104-105 -0.8288173259924925 2216 106-107 -0.8197970828782957 2216 108-109 -0.7215670687647844 2216 110-111 -0.7942991866654197 2216 112-113 -0.5761899705206801 2216 114-115 -0.7506719118681247 2216 116-117 -0.9878536247743632 2216 118-119 -0.9393129989096778 2216 120-121 -0.9677879241167879 2216 122-123 -0.9996493850292651 2216 124-125 -1.437840405073871 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62.0 3 3.0 4 11.0 5 31.0 6 60.0 7 131.0 8 239.0 9 516.0 10 1247.0 11 1843.0 12 2160.0 13 3710.0 14 6850.0 15 7566.0 16 2474.0 17 1479.0 18 1121.0 19 977.0 20 937.0 21 940.0 22 1032.0 23 1034.0 24 1361.0 25 1626.0 26 2073.0 27 2676.0 28 3452.0 29 4394.0 30 5732.0 31 7308.0 32 9468.0 33 12714.0 34 18455.0 35 30521.0 36 64842.0 37 214890.0 38 217131.0 39 2989.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.29091588808634 20.719517061990402 15.94108711529088 22.04847993463238 2 13.194281253203584 20.765548729999765 45.289446499120736 20.75072351767591 3 18.864767252052268 27.44414916687038 29.992666251350435 23.69841732972692 4 11.162280874687527 18.307875499759486 39.89275378318916 30.637089842363835 5 11.186808987096091 39.02883520551342 34.57429420153217 15.210061605858321 6 27.47332644644392 38.08849390037142 20.490809156934336 13.947370496250326 7 26.342825149237843 33.34127165624433 22.59756645716854 17.718336737349283 8 24.281647491148245 38.24037346917854 20.706405595729077 16.77157344394414 9 25.87125722531957 18.221132236162475 22.79896854373832 33.10864199477963 10-11 23.47114997910276 26.905158069883527 30.301314554731057 19.322377396282654 12-13 26.733800695523257 24.99136510239648 29.750337115865342 18.524497086214918 14-15 20.652782487323655 29.822807169725024 27.609040225217058 21.915370117734266 16-17 18.631757995566627 30.633328838451586 31.493428404025202 19.24148476195658 18-19 19.77454637216014 27.458974379194235 34.254362791871365 18.512116456774255 20-21 20.862367551914602 27.450782464961783 32.165208778244285 19.521641204879327 22-23 19.457348630675487 29.74031371169783 32.48077646444661 18.321561193180074 24-25 20.10180504845794 27.75358604537461 34.27573317772117 17.86887572844627 26-27 18.051194297024704 29.418504703850612 33.67878180914905 18.851519189975633 28-29 18.138883850770043 29.641040603733117 31.687629622035946 20.532445923460898 30-31 19.22356893329443 27.83165498261192 33.223537390289486 19.721238693804167 32-33 18.234853443313277 29.032654895868653 34.22447579468658 18.508015866131487 34-35 18.732996348897178 29.363067872660892 33.73634779317251 18.167587985269417 36-37 18.14913532737696 28.765170213940433 33.33922136092295 19.746473097759658 38-39 19.596880396811002 29.5621830913722 32.08885664492828 18.75207986688852 40-41 21.294761495453866 27.119729361017576 33.522880507211525 18.062628636317037 42-43 18.60288145350167 29.693480849453124 33.32707730401936 18.37656039302584 44-45 18.49524094912902 29.809874537697834 31.99091561457602 19.703968898597125 46-47 20.558942047614163 28.258826127070996 31.372199572592287 19.810032252722557 48-49 19.990300525979606 29.075001380006466 30.75987098910978 20.17482710490415 50-51 18.7573633202167 30.67052542760486 30.86443605050035 19.707675201678086 52-53 18.859641513748805 29.67431847394942 31.486464108003247 19.979575904298525 54-55 19.24769933207687 28.7642239237921 31.56476961777764 20.423307126353393 56-57 19.9053709851669 29.2536925030163 29.373240491755446 21.46769602006135 58-59 20.097467885278093 30.006860603575397 29.043458375062098 20.852213136084412 60-61 19.39074685949957 28.013894693677994 32.4491566189053 20.14620182791714 62-63 19.90079724944997 27.96957677173116 31.854018973117476 20.2756070057014 64-65 20.349654209808296 29.347532942725792 29.874774270370867 20.428038577095048 66-67 20.189967747277443 30.77390762631002 29.04243322740141 19.993691399011126 68-69 21.09288626380992 28.11877518511801 31.55917073440001 19.229167816672053 70-71 21.845581219294857 27.970523061879486 30.667528842135145 19.51636687669051 72-73 20.54048938972171 29.565810536940802 30.111110234916527 19.782589838420957 74-75 19.687881966075498 30.16623163605681 31.37543273059908 18.77045366726861 76-77 20.028152131912847 28.536010283019614 31.94699198019099 19.48884560487655 78-79 19.674712761511227 28.247943790365188 31.368098981949515 20.70924446617407 80-81 20.181608850967187 29.259291386393926 30.99108121535198 19.568018547286908 82-83 19.692692274329517 29.132488506517575 32.02017175166193 19.15464746749099 84-85 19.512739431121908 29.000007885751234 31.91071752450497 19.576535158621887 86-87 18.32396243228111 30.936432959285863 29.797178478207726 20.942426130225293 88-89 19.494602203278895 28.95726711405162 30.86845778363076 20.679672899038724 90-91 19.767131929658543 29.05378124753568 31.75971926504219 19.419367557763582 92-93 19.007499349425522 30.27757844351042 31.650487733713952 19.064434473350104 94-95 18.9524526754488 30.377926118105364 30.899728334798777 19.769892871647063 96-97 20.358880538754526 29.575588868473556 30.4099802067644 19.655550386007523 98-99 20.393503641288703 28.699663668731446 31.74848887504485 19.158343814935 100-101 19.556111062920408 29.016331390809945 31.67879758065152 19.748759965618124 102-103 19.054622362308542 30.009218465003347 30.889506958009065 20.046652214679046 104-105 19.70124960373225 29.228945762182573 30.70353494490104 20.36626968918414 106-107 19.631557364121004 28.6130563704615 30.654395760386105 21.100990505031387 108-109 19.021216697355502 29.73144968279441 30.283375587983645 20.963958031866447 110-111 18.709475434088976 29.98048985141058 30.783055664646252 20.526979049854184 112-113 19.52978177565266 28.566482677146105 30.368523202158983 21.53521234504226 114-115 19.282380718802198 29.33983883967087 28.880710127486687 22.497070314040254 116-117 19.855531328488794 29.409231958173482 27.91762446819836 22.81761224513936 118-119 19.67635197688521 28.252606643293227 30.03329374673726 22.0377476330843 120-121 19.06028138092884 28.149842717823393 30.824073438829497 21.965802462418274 122-123 20.03469757905528 28.990458165759797 29.572194621875248 21.402649633309675 124-125 20.58165989141192 29.180463608799023 28.68791376040439 21.549962739384668 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 105.0 1 71.0 2 111.5 3 150.5 4 69.0 5 34.0 6 87.5 7 227.0 8 387.5 9 425.5 10 337.5 11 265.5 12 292.5 13 367.5 14 467.0 15 567.0 16 693.5 17 845.5 18 1014.0 19 1207.0 20 1455.0 21 1753.5 22 2180.5 23 2665.0 24 3121.0 25 3782.5 26 4617.0 27 5624.5 28 6817.5 29 8345.5 30 10481.0 31 13538.5 32 17450.5 33 22797.0 34 30221.0 35 37524.0 36 42580.5 37 43480.5 38 39204.0 39 32416.0 40 27055.0 41 23606.0 42 20650.5 43 18614.0 44 17564.5 45 17009.0 46 16457.0 47 16860.0 48 18494.0 49 17490.0 50 15046.5 51 13473.0 52 11653.5 53 10502.5 54 9630.0 55 8458.0 56 7162.0 57 5910.0 58 4854.5 59 3875.5 60 2944.0 61 2211.0 62 1713.5 63 1256.5 64 887.5 65 704.5 66 520.5 67 422.5 68 325.0 69 200.0 70 163.0 71 135.0 72 110.5 73 111.5 74 82.0 75 57.5 76 46.5 77 25.5 78 14.5 79 9.5 80 5.0 81 2.0 82 2.0 83 1.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 2.0 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016086932521626674 2 0.0 3 0.0 4 0.0 5 0.13184976066744997 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.885751236091507E-5 18-19 0.0 20-21 0.005204595815820394 22-23 2.3657253708274522E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 7.885751236091506E-4 92-93 0.0 94-95 3.942875618045753E-4 96-97 0.0 98-99 0.001182862685413726 100-101 0.0 102-103 2.3657253708274522E-4 104-105 0.002208010346105622 106-107 0.0055200258652640544 108-109 3.942875618045753E-4 110-111 0.004100590642767583 112-113 0.008122323773174251 114-115 0.004416020692211244 116-117 0.001182862685413726 118-119 3.154300494436603E-4 120-121 5.520025865264055E-4 122-123 7.885751236091506E-4 124-125 0.001182862685413726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 634055.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.71425445885347 #Duplication Level Percentage of deduplicated Percentage of total 1 89.78920926548557 68.88112247251705 2 7.21972536454644 11.077116974777084 3 1.410410937611461 3.2459587067842723 4 0.5372655037466914 1.648636902655511 5 0.26309493209970125 1.0091565783965628 6 0.15785031114432616 0.7265621361321017 7 0.11059981727933471 0.5939207768108707 8 0.07698521548559113 0.4724690728265044 9 0.05498001467178018 0.37959757521141974 >10 0.3240177846498984 4.686419105877472 >50 0.02977888880396965 1.4921474087942646 >100 0.02361752688728211 3.656529447689464 >500 0.0012322187939917243 0.7026636794270527 >1k 0.0012322187939917243 1.4276991621004373 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2298 0.3624291268107656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1748 0.27568586321375904 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1428 0.22521705530277342 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1385 0.21843530923973473 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1202 0.18957345971563982 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1001 0.15787273974655194 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 903 0.14241666732381258 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 821 0.12948403529662253 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 788 0.12427943948080213 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 765 0.12065199391220005 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 2.365725370827452E-4 0.0 26-27 0.0 0.0 0.0 8.674326359700657E-4 0.0 28-29 0.0 0.0 0.0 0.0012617201977746409 0.0 30-31 0.0 0.0 0.0 0.0033908730315193474 0.0 32-33 0.0 0.0 0.0 0.007649178699008761 1.5771502472183014E-4 34-35 0.0 0.0 0.0 0.012222914415941834 1.5771502472183014E-4 36-37 0.0 0.0 0.0 0.019477805553146023 1.5771502472183014E-4 38-39 0.0 0.0 0.0 0.034855020463524455 1.5771502472183014E-4 40-41 0.0 0.0 0.0 0.05819684412235532 1.5771502472183014E-4 42-43 0.0 0.0 0.0 0.07925179992271963 1.5771502472183014E-4 44-45 0.0 0.0 0.0 0.09983361064891846 1.5771502472183014E-4 46-47 0.0 0.0 0.0 0.12790688504940423 1.5771502472183014E-4 48-49 0.0 0.0 0.0 0.16000189258029668 1.5771502472183014E-4 50-51 0.0 0.0 0.0 0.19856321612478411 1.5771502472183014E-4 52-53 0.0 0.0 0.0 0.23255080395233851 1.5771502472183014E-4 54-55 0.0 0.0 0.0 0.2730835653058489 1.5771502472183014E-4 56-57 0.0 0.0 0.0 0.3229215131179472 1.5771502472183014E-4 58-59 0.0 0.0 0.0 0.38640181056848383 1.5771502472183014E-4 60-61 0.0 0.0 0.0 0.4591872944776084 1.5771502472183014E-4 62-63 0.0 0.0 0.0 0.5296070530159056 1.5771502472183014E-4 64-65 0.0 0.0 0.0 0.5959262209114351 1.5771502472183014E-4 66-67 0.0 0.0 0.0 0.6782534638162305 3.154300494436603E-4 68-69 0.0 0.0 0.0 0.7846322479911049 3.154300494436603E-4 70-71 0.0 0.0 0.0 0.8929824699750022 3.154300494436603E-4 72-73 0.0 0.0 0.0 1.0103224483680437 3.154300494436603E-4 74-75 0.0 0.0 0.0 1.1260852765138671 3.154300494436603E-4 76-77 0.0 0.0 0.0 1.2502070009699473 3.154300494436603E-4 78-79 0.0 0.0 0.0 1.3980648366466633 3.154300494436603E-4 80-81 0.0 0.0 0.0 1.5604324545977872 3.154300494436603E-4 82-83 0.0 0.0 0.0 1.7235886476725204 3.154300494436603E-4 84-85 0.0 0.0 0.0 1.8833539677157345 3.154300494436603E-4 86-87 0.0 0.0 0.0 2.0476141659635205 3.154300494436603E-4 88-89 0.0 0.0 0.0 2.2352161878701375 3.154300494436603E-4 90-91 0.0 0.0 0.0 2.4484469012940515 3.154300494436603E-4 92-93 0.0 0.0 0.0 2.6743736742080735 3.154300494436603E-4 94-95 0.0 0.0 0.0 2.895016993793914 3.154300494436603E-4 96-97 0.0 0.0 0.0 3.1162911734786416 3.154300494436603E-4 98-99 0.0 0.0 0.0 3.3641403348289973 3.154300494436603E-4 100-101 0.0 0.0 0.0 3.63067872660889 3.154300494436603E-4 102-103 0.0 0.0 0.0 3.9173257840408167 3.154300494436603E-4 104-105 0.0 0.0 0.0 4.213120312906609 3.154300494436603E-4 106-107 0.0 0.0 0.0 4.500555945462144 3.154300494436603E-4 108-109 0.0 0.0 0.0 4.800529922483065 3.154300494436603E-4 110-111 0.0 0.0 0.0 5.135674350016954 3.154300494436603E-4 112-113 0.0 0.0 0.0 5.525388176104597 3.154300494436603E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGATCG 35 0.007746907 51.079315 5 ATAACGC 75 1.454111E-6 47.674023 3 TATAACG 95 7.3877545E-6 37.637394 2 GTACCTG 725 0.0 35.352917 1 TAACGCA 85 1.6940714E-4 35.054432 4 TACCTGG 745 0.0 34.39569 2 TCGAATA 70 0.0027668849 34.052876 4 TACGTGG 70 0.0027668849 34.052876 5 CGATTGA 35 0.0027888904 34.000507 14-15 GTATCAA 5075 0.0 32.416626 1 TAGGACC 1115 0.0 30.464344 4 GTACATG 3860 0.0 29.648907 1 TACATGG 3885 0.0 29.451136 2 GGTATCA 3665 0.0 29.437397 1 ACCTGGG 830 0.0 29.437277 3 ACATGGG 3735 0.0 28.559744 3 ACGTAGA 85 0.0071452153 28.050196 1 ATCAACG 5825 0.0 28.031511 3 TCAACGC 5830 0.0 28.007471 4 TATCAAC 5855 0.0 27.887882 2 >>END_MODULE