FastQCFastQC Report
Fri 27 May 2016
SRR939899_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939899_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171589
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC5750.01366.0878108-109
CCGTTCG652.9056537E-81169.4796108-109
CCGCGCC6850.01109.7252108-109
CGTCGCC3000.0760.16174108-109
CGTTGTT805.3330135E-5739.9804110-111
CTCCGCC3150.0657.76044110-111
CCGCTCG1355.394777E-7563.08276108-109
CCTCGCC8950.0481.29422108-109
CGACGCC1102.2099998E-4460.7041108-109
CCGCGCG3300.0460.70404108-109
TTGCGTG1302.285533E-4455.3726110-111
TTGTTCG2308.141797E-9440.6735108-109
CGCCGCC5100.0406.2638110-111
TTTTTCG5950.0362.1543392-93
CGGCGCC2153.4618333E-6353.56357108-109
CGCCTCC3401.3460522E-10348.2261110-111
GCGCTCG1505.59729E-4337.84967108-109
CGTCGTT1806.059401E-4328.88022110-111
CCCCTCG3650.0323.0278392-93
AATCGCC1601.5454134E-8316.73404108-109
Produced by FastQC (version 0.11.5)