##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939899_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1171589 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.471595414432876 33.0 31.0 34.0 28.0 34.0 2 31.61192278179464 34.0 31.0 34.0 28.0 34.0 3 31.702084946171396 34.0 31.0 34.0 28.0 34.0 4 34.96916666168767 37.0 35.0 37.0 32.0 37.0 5 35.00410468176127 37.0 35.0 37.0 32.0 37.0 6 34.993663306842244 37.0 35.0 37.0 32.0 37.0 7 34.99702796799902 37.0 36.0 37.0 32.0 37.0 8 34.981098320315404 37.0 36.0 37.0 32.0 37.0 9 36.60975478602138 39.0 38.0 39.0 33.0 39.0 10-11 36.59873940434743 39.0 38.0 39.0 33.0 39.0 12-13 36.533248861162065 39.0 38.0 39.0 33.0 39.0 14-15 37.99337438299608 41.0 39.0 41.0 33.0 41.0 16-17 37.97597450983237 41.0 39.0 41.0 33.0 41.0 18-19 37.960552292655535 41.0 39.0 41.0 33.0 41.0 20-21 38.010736700327506 41.0 39.0 41.0 33.0 41.0 22-23 37.97321287584639 41.0 39.0 41.0 33.0 41.0 24-25 37.98025331408881 41.0 39.0 41.0 33.0 41.0 26-27 37.949615436812735 41.0 39.0 41.0 33.0 41.0 28-29 37.887728546444194 40.0 38.0 41.0 33.0 41.0 30-31 37.81542759448919 40.0 38.0 41.0 32.5 41.0 32-33 37.71387064917817 40.0 38.0 41.0 32.0 41.0 34-35 37.624812114145826 40.0 38.0 41.0 32.0 41.0 36-37 37.78594797322269 40.5 38.0 41.0 33.0 41.0 38-39 37.754178299727975 41.0 38.0 41.0 32.0 41.0 40-41 37.69085575231587 41.0 38.0 41.0 32.0 41.0 42-43 37.579824494767365 40.5 38.0 41.0 32.0 41.0 44-45 37.44705353157123 40.0 38.0 41.0 32.0 41.0 46-47 37.32938214681087 40.0 38.0 41.0 31.0 41.0 48-49 37.214618778428274 40.0 38.0 41.0 31.0 41.0 50-51 37.072323570808535 40.0 37.0 41.0 31.0 41.0 52-53 36.924775241146854 40.0 37.0 41.0 30.5 41.0 54-55 36.74878092914837 40.0 36.5 41.0 30.0 41.0 56-57 36.5566435840555 40.0 36.0 41.0 30.0 41.0 58-59 36.361749299455695 40.0 35.5 41.0 30.0 41.0 60-61 36.11151265503517 39.0 35.0 41.0 30.0 41.0 62-63 35.83258378151382 39.0 35.0 41.0 30.0 41.0 64-65 35.524865801915176 38.5 35.0 41.0 29.0 41.0 66-67 35.212423896093256 38.0 35.0 40.0 29.0 41.0 68-69 34.85229760607176 37.0 35.0 40.0 29.0 41.0 70-71 34.48006041367749 37.0 34.5 39.5 28.0 41.0 72-73 34.09242020879336 36.0 34.0 39.0 28.0 41.0 74-75 33.68793920052168 36.0 34.0 39.0 27.5 40.5 76-77 33.27415501511196 35.0 34.0 37.5 26.5 39.5 78-79 32.86683342025232 35.0 34.0 37.0 26.0 39.0 80-81 32.48109746677376 35.0 34.0 36.5 26.0 39.0 82-83 32.33035305042981 35.0 34.0 36.0 26.0 38.0 84-85 31.97053531571225 35.0 34.0 36.0 25.5 37.0 86-87 31.345523899592777 35.0 34.0 35.5 10.5 37.0 88-89 16.074063515447822 18.0 16.5 18.5 2.0 19.0 90-91 2.713839068137376 2.0 2.0 2.0 2.0 2.0 92-93 2.272858485356213 2.0 2.0 2.0 2.0 2.0 94-95 2.093353556579995 2.0 2.0 2.0 2.0 2.0 96-97 2.0000264597909334 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.03850679587685235 1101 2 0.05257237464529396 1101 3 0.14116089326375558 1101 4 0.060955062303087004 1101 5 0.05388153638104143 1101 6 -0.006439865256403721 1101 7 0.10938374337276713 1101 8 0.06611311318336277 1101 9 0.11590793312884529 1101 10-11 0.02834257462401979 1101 12-13 0.07701543833920255 1101 14-15 0.05916217597289375 1101 16-17 0.10231282106254014 1101 18-19 0.07653516285329687 1101 20-21 -0.023332729485154857 1101 22-23 0.04260373006719931 1101 24-25 -0.006590815124916105 1101 26-27 0.024102750562292385 1101 28-29 0.006202322445396646 1101 30-31 0.016666527494685113 1101 32-33 -0.0408620689773187 1101 34-35 -0.0903336909651955 1101 36-37 -0.04297160391075039 1101 38-39 -0.022947227227717804 1101 40-41 -0.17985572971059582 1101 42-43 -0.13974303370181218 1101 44-45 -0.07032654701749408 1101 46-47 -0.1156969903712266 1101 48-49 -0.1173706147402811 1101 50-51 -0.2700241512686432 1101 52-53 -0.2322218478562874 1101 54-55 -0.12363719529614059 1101 56-57 -0.1628939124226676 1101 58-59 -0.10408289540363569 1101 60-61 -0.2497252350721766 1101 62-63 -0.28387496912618815 1101 64-65 -0.17121091922841458 1101 66-67 -0.10572076233918892 1101 68-69 -0.08735505484237649 1101 70-71 -0.10264139994172439 1101 72-73 -0.15490275561941047 1101 74-75 -0.2682910509816381 1101 76-77 -0.29425926979157424 1101 78-79 -0.18168926874906077 1101 80-81 -0.12518720076036516 1101 82-83 -0.11368482531685231 1101 84-85 -0.12334924786814838 1101 86-87 -0.06041999707563761 1101 88-89 0.61742359911187 1101 90-91 -0.05437776939030803 1101 92-93 -0.16461134095565866 1101 94-95 -0.011967513167911115 1101 96-97 -2.649753829322421E-4 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.0696435907707631 1102 2 0.06150100311851503 1102 3 0.10451358526919918 1102 4 -0.025976875672490962 1102 5 0.04701974963310818 1102 6 0.17069198455096313 1102 7 0.09875564071430887 1102 8 0.10387530179944093 1102 9 0.194074162148965 1102 10-11 0.21005853243106287 1102 12-13 0.15129076593521518 1102 14-15 0.18160737431941953 1102 16-17 0.22169928532566274 1102 18-19 0.05287030427382433 1102 20-21 0.15053434096635954 1102 22-23 0.025291135430130396 1102 24-25 0.035178874177979935 1102 26-27 0.02075171969703149 1102 28-29 0.0472340821678614 1102 30-31 0.1756088923194099 1102 32-33 0.1888017418060386 1102 34-35 0.043533443376318814 1102 36-37 0.014880797395164791 1102 38-39 0.04244579215012578 1102 40-41 0.01950025901920327 1102 42-43 0.0303577208391701 1102 44-45 0.10783073740164895 1102 46-47 0.024103928367075866 1102 48-49 0.01653964535594099 1102 50-51 0.03868118163023837 1102 52-53 -0.029807125043085136 1102 54-55 -0.059668923073594726 1102 56-57 -0.04858031471248836 1102 58-59 -0.016152676069246752 1102 60-61 -0.06849324406788782 1102 62-63 -0.11075062000563918 1102 64-65 -0.06768035744358514 1102 66-67 0.15091421488402545 1102 68-69 0.014231653480578643 1102 70-71 0.10078118773133582 1102 72-73 0.13766076702001584 1102 74-75 0.17945011782214948 1102 76-77 0.12371714201785977 1102 78-79 0.045356648998939875 1102 80-81 0.06441118665586032 1102 82-83 0.12746481146845667 1102 84-85 0.19341900390958955 1102 86-87 0.3467126833018277 1102 88-89 1.1835545480701448 1102 90-91 0.13478456872838684 1102 92-93 -0.09436587554890297 1102 94-95 0.039805753975375335 1102 96-97 -2.649753829322421E-4 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.06511972407089317 1103 2 -0.024074723915433083 1103 3 0.06439934501064926 1103 4 -0.009894182328942236 1103 5 0.025659917113301844 1103 6 -0.25032029603388395 1103 7 -0.09607508823544464 1103 8 -0.1084687267210569 1103 9 -0.10092468559177092 1103 10-11 -0.08085638918754512 1103 12-13 0.0015484277651722778 1103 14-15 -0.022784850568264403 1103 16-17 -0.012417063706159581 1103 18-19 0.026660917942123774 1103 20-21 0.011129538405292294 1103 22-23 0.010431502820360095 1103 24-25 -0.010527814756443377 1103 26-27 -0.024074289154540907 1103 28-29 0.039473685457487306 1103 30-31 -0.026655123595652697 1103 32-33 -0.07312327388134321 1103 34-35 -0.015480451759756875 1103 36-37 -0.030128115260808386 1103 38-39 -0.17756527231531294 1103 40-41 -0.0992084748283375 1103 42-43 -0.040857801620170164 1103 44-45 -0.06585672823881339 1103 46-47 -0.15380524861439682 1103 48-49 -0.14871168598940443 1103 50-51 -0.12682316086008427 1103 52-53 -0.08569204627297466 1103 54-55 -0.2813781816849996 1103 56-57 -0.21787756844830142 1103 58-59 -0.1998779857909767 1103 60-61 -0.08886654136188099 1103 62-63 -0.13525122667707734 1103 64-65 -0.01650599007556508 1103 66-67 -0.1153369790231551 1103 68-69 -0.1627459982428121 1103 70-71 -0.0049526556502499375 1103 72-73 -0.004357095775063158 1103 74-75 -0.01628957709291967 1103 76-77 0.011767692881086589 1103 78-79 0.03540539789527486 1103 80-81 -0.0520384943210388 1103 82-83 -0.053781926110744394 1103 84-85 0.023171830415439132 1103 86-87 -0.08214485164078056 1103 88-89 0.6476896109029795 1103 90-91 -0.481027031329365 1103 92-93 -0.3064870876701149 1103 94-95 -0.10814433515118438 1103 96-97 -2.649753829322421E-4 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.060446826874631654 1104 2 -0.04802332204627291 1104 3 -0.10032028115757541 1104 4 -0.07181007017940999 1104 5 0.09575337505722814 1104 6 -0.08560066986565573 1104 7 -0.06394892001114272 1104 8 0.027045291970530627 1104 9 -0.0010415080216787942 1104 10-11 0.048232395859187704 1104 12-13 0.1186629137464763 1104 14-15 0.0881964578429475 1104 16-17 0.175374992368603 1104 18-19 0.09354175906361206 1104 20-21 0.023395893545476554 1104 22-23 -0.09762924484319058 1104 24-25 -1.3796064855853274E-5 1104 26-27 0.07843739308844988 1104 28-29 0.09496433966310036 1104 30-31 0.0670948764043473 1104 32-33 0.04807999714669364 1104 34-35 0.12061767908137 1104 36-37 0.08903076324386916 1104 38-39 0.11887164357253965 1104 40-41 0.1131162915268007 1104 42-43 -0.0011381754519419474 1104 44-45 0.08776476708828795 1104 46-47 0.04625316260055712 1104 48-49 -0.0012233682323952166 1104 50-51 -0.12215026366382631 1104 52-53 -0.10613597150662457 1104 54-55 -0.05503472374108043 1104 56-57 -0.10543597966325535 1104 58-59 -0.07984293906201145 1104 60-61 -0.0748478497731 1104 62-63 -0.10049655377988387 1104 64-65 -0.10821159755219867 1104 66-67 -0.07999819397642227 1104 68-69 -0.11076001693439963 1104 70-71 -0.0689129360240841 1104 72-73 -0.07707905839188811 1104 74-75 -0.10040172662562696 1104 76-77 -0.08490286786658174 1104 78-79 0.011456799764438585 1104 80-81 -0.10636092422757315 1104 82-83 -0.003548281250928653 1104 84-85 0.08041482106964537 1104 86-87 -0.05410746846321146 1104 88-89 0.11176671370671798 1104 90-91 -0.5928845079648788 1104 92-93 -0.3064870876701149 1104 94-95 -0.10814433515118438 1104 96-97 -2.649753829322421E-4 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.01942556089263192 1105 2 0.002907499750204323 1105 3 -0.03813498374015012 1105 4 0.11708294167863897 1105 5 0.14516100951373545 1105 6 0.1324690961405608 1105 7 0.08835907786310315 1105 8 -0.05239463137355216 1105 9 0.1980511554491997 1105 10-11 0.12700701512945045 1105 12-13 0.2243994587013063 1105 14-15 0.016277691369715797 1105 16-17 0.017727786712818272 1105 18-19 0.14693064959274693 1105 20-21 0.16154982403982387 1105 22-23 -0.05189403519356972 1105 24-25 0.11817732167283168 1105 26-27 0.11840579533873807 1105 28-29 -0.013691591776023415 1105 30-31 -0.0067874261717904005 1105 32-33 0.0076809757442291016 1105 34-35 0.088585955182225 1105 36-37 0.0036168588919736067 1105 38-39 0.014980034120455343 1105 40-41 0.0312988203667075 1105 42-43 -0.0031528758872880758 1105 44-45 -0.14501583488608816 1105 46-47 0.09638364003591704 1105 48-49 0.05282881334764511 1105 50-51 -0.022597892859636204 1105 52-53 0.09922792935602587 1105 54-55 0.07293733684286252 1105 56-57 0.1687236503699765 1105 58-59 0.16703201061241657 1105 60-61 0.11429930622812634 1105 62-63 0.16358017996965657 1105 64-65 0.17943109222218112 1105 66-67 0.16088314771675982 1105 68-69 0.22073855784537244 1105 70-71 0.03355966002394695 1105 72-73 0.05048721032251535 1105 74-75 0.04578399755265394 1105 76-77 0.14564998874713098 1105 78-79 0.05565355665031291 1105 80-81 -0.014982097471012423 1105 82-83 -0.002971046929701515 1105 84-85 -0.05467193350796862 1105 86-87 0.08226225271969057 1105 88-89 0.9174422305168015 1105 90-91 -0.7365760967499257 1105 92-93 -0.3064870876701149 1105 94-95 -0.10814433515118438 1105 96-97 -2.649753829322421E-4 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.42410835647857326 1106 2 -0.30394411041357117 1106 3 -0.27864787936875146 1106 4 -0.41550199369921614 1106 5 -0.4162194177017966 1106 6 -0.32684635178730304 1106 7 -0.4705579230371484 1106 8 -0.3147008184116018 1106 9 -0.3017732381986846 1106 10-11 -0.4611967474876266 1106 12-13 -0.3799166393156739 1106 14-15 -0.3894628424275126 1106 16-17 -0.4676109755504143 1106 18-19 -0.4005921817017253 1106 20-21 -0.3964369733907631 1106 22-23 -0.42133984656238965 1106 24-25 -0.4900556044359021 1106 26-27 -0.6229793717670802 1106 28-29 -0.34804740283214386 1106 30-31 -0.36566394050912976 1106 32-33 -0.2687374117385062 1106 34-35 -0.3032795372644017 1106 36-37 -0.15462668259605294 1106 38-39 -0.2351721196494836 1106 40-41 -0.24196046315278608 1106 42-43 -0.5961040994537612 1106 44-45 -0.2705781619747185 1106 46-47 -0.45239032480002805 1106 48-49 -0.40015707066658734 1106 50-51 -0.4146197358540604 1106 52-53 -0.3422732663142156 1106 54-55 -0.24148114609599958 1106 56-57 -0.44027852043394233 1106 58-59 -0.30458937748420567 1106 60-61 -0.2105049675729589 1106 62-63 -0.35266530026517273 1106 64-65 -0.3590378902978273 1106 66-67 -0.27855849979010827 1106 68-69 -0.3372181215323167 1106 70-71 -0.31712243981328214 1106 72-73 -0.26866951711239295 1106 74-75 -0.36097265022576863 1106 76-77 -0.43461231809821754 1106 78-79 -0.2827782308972715 1106 80-81 -0.3274361592113735 1106 82-83 -0.2947613265338518 1106 84-85 -0.42146521668843206 1106 86-87 -0.3911423545195021 1106 88-89 0.509730856919635 1106 90-91 -0.17812337468688844 1106 92-93 -0.2798395232002009 1106 94-95 -0.10814433515118438 1106 96-97 -2.649753829322421E-4 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.017872684779906223 1107 2 -0.022739280610259982 1107 3 0.07749882679663855 1107 4 0.046667728941422126 1107 5 0.018359009974929563 1107 6 0.002717021179584833 1107 7 -0.023626381923470774 1107 8 -0.07437781763015039 1107 9 -0.0633558190661816 1107 10-11 -0.02142141132509323 1107 12-13 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0.3497540397857435 2216 32-33 0.328099456064713 2216 34-35 0.48485582912869063 2216 36-37 0.4514353065681007 2216 38-39 0.33730747509093106 2216 40-41 0.3939619503731464 2216 42-43 0.36821310970965015 2216 44-45 0.3675045184184782 2216 46-47 0.3385300742612074 2216 48-49 0.5182433034885392 2216 50-51 0.4603313112449712 2216 52-53 0.47060958763287175 2216 54-55 0.4003766129740214 2216 56-57 0.3228934021542571 2216 58-59 0.43105101098348086 2216 60-61 0.46440339541386777 2216 62-63 0.35881489458850524 2216 64-65 0.3347760126077901 2216 66-67 0.34740935893253067 2216 68-69 0.36511161393887903 2216 70-71 0.309555552934647 2216 72-73 0.30693418837568487 2216 74-75 0.24544320854194268 2216 76-77 0.3186571721999485 2216 78-79 0.3621805359685908 2216 80-81 0.40517830605389804 2216 82-83 0.4731851591409111 2216 84-85 0.459413146270208 2216 86-87 0.575357801848039 2216 88-89 -0.46766220036159645 2216 90-91 -0.7365760967499257 2216 92-93 -0.3064870876701149 2216 94-95 -0.10814433515118438 2216 96-97 -2.649753829322421E-4 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20974.0 3 3278.0 4 2312.0 5 2021.0 6 2187.0 7 1995.0 8 2250.0 9 2624.0 10 2925.0 11 3546.0 12 3957.0 13 4402.0 14 4808.0 15 5333.0 16 5865.0 17 7099.0 18 8759.0 19 10920.0 20 12082.0 21 16645.0 22 19202.0 23 25114.0 24 38081.0 25 65200.0 26 138192.0 27 371181.0 28 378014.0 29 12231.0 30 392.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63858742272247 19.09304372096358 14.488015848561227 23.78035300775272 2 14.530717735857431 19.8942972224854 41.31148604414354 24.26349899751363 3 18.987548210871815 25.017824254135014 28.312685660419568 27.681941874573607 4 12.602275812955021 17.039479189615218 37.42650060029135 32.93174439713841 5 14.190221967185677 37.717684791908965 33.71563197543452 14.37646126547084 6 31.669467705825166 36.355752742642686 18.34329274173793 13.631486809794218 7 29.335628791325284 31.802876264628637 20.748999862579794 18.11249508146628 8 26.085768985540152 35.92684806702692 19.914236135709707 18.073146811723223 9 26.388434852153786 17.744362570833285 20.14016860861616 35.72703396839677 10-11 24.9658367624603 26.872960555010707 27.36882895847577 20.792373724053224 12-13 25.82218872485421 23.999332518470972 27.70633031970829 22.472148436966528 14-15 22.4055299303168 26.11549452537923 25.2023748962946 26.276600648009367 16-17 22.67186020299859 29.061284098586142 26.032906717355853 22.233948981059413 18-19 22.816217688096827 27.919498835264882 27.29679874964896 21.967484726989333 20-21 23.737168322502367 27.12814969056308 26.790993403259833 22.343688583674723 22-23 23.12132067693143 27.41457910293868 26.731911835374593 22.7321883847553 24-25 22.94819258289383 27.33219584683707 27.023043063736512 22.69656850653258 26-27 22.606861279851554 27.769507907636555 26.8899332445081 22.733697568003798 28-29 22.54723008317411 27.798521396560282 26.757319291878435 22.896929228387172 30-31 22.776801420976128 27.371629470744434 26.913747056348257 22.93782205193118 32-33 22.501133084268183 27.60043906481925 26.923277178445193 22.975150672467375 34-35 22.935441923508126 27.68147690080604 26.75107343576691 22.632007739918922 36-37 22.87442343212802 27.30925888622963 26.777843405702345 23.038474275940008 38-39 22.72716797443472 27.566962475748745 26.71679232222221 22.98907722759432 40-41 23.159572170786856 27.228447860128423 26.909479348133175 22.702500620951547 42-43 22.715005006021734 27.521169966600915 26.744105654798737 23.01971937257861 44-45 22.656950505190608 27.6199600968771 26.701377404589927 23.021711993342368 46-47 22.909569823547336 27.44149185422533 26.638010428571796 23.010927893655538 48-49 22.904146256317286 27.421864530760416 26.65572424412357 23.018264968798732 50-51 22.703672280699358 27.763940907554534 26.597745965305208 22.9346408464409 52-53 22.880037282698968 27.56982184025285 26.670316979759967 22.87982389728821 54-55 22.681074095776484 27.292230990596817 26.980897025917756 23.045797887708943 56-57 22.811412534600446 27.499148592211093 26.65589212599299 23.033546747195476 58-59 22.938760947738498 27.249359630382326 26.616842595824984 23.19503682605419 60-61 22.75465201533985 27.263144327917043 27.086119791155433 22.896083865587677 62-63 22.772491035678893 27.345468419385977 26.91118643141921 22.97085411351592 64-65 22.65141615361701 27.289134670946897 26.777095039301322 23.28235413613477 66-67 22.733740245085947 27.63601399466878 26.569428357555424 23.06081740268985 68-69 22.906644656654045 27.167698883907992 27.003434654503117 22.922221804934843 70-71 22.95702673889905 27.21223056891111 26.79856161162319 23.032181080566648 72-73 23.056848434049826 27.251578838654172 26.760493654344653 22.931079072951352 74-75 22.72499144324503 27.562950830026573 26.813242527883073 22.89881519884533 76-77 22.889127501197095 27.157945320415266 26.95108950323023 23.001837675157415 78-79 22.887472862698807 27.115407841575774 26.902022339754954 23.09509695597046 80-81 22.851272929329312 27.511738331445585 26.775601341426047 22.861387397799056 82-83 22.860010771750698 27.540174277025187 26.781798643373968 22.818016307850147 84-85 22.84094507544881 27.28815309805743 26.954887763541652 22.91601406295211 86-87 22.7256354666348 27.54762798955257 26.640604994964068 23.086131548848563 88-89 31.921529610700432 17.32456487780457 32.625002244395084 18.128903267099915 90-91 30.526320055205886 12.992603707871933 15.047309407694554 41.43376682922762 92-93 22.911446402902197 16.0196586619635 32.885313717688135 28.183581217446168 94-95 14.794655422517305 6.2973212967764525 25.379092033908652 53.52893124679758 96-97 25.094941895144217 6.752958069166783 13.54609746175495 54.606002573934056 98-99 57.16205801658497 14.18724374980733 18.559758315607755 10.090939917999938 100-101 30.676938419169836 6.888361677360741 11.64293623303617 50.791763670433255 102-103 23.094577367171823 1.9523960311470818 35.88630749393384 39.066719107747254 104-105 42.95101125739363 4.94010139831548 36.623327064082645 15.485560280208249 106-107 4.918535236383647 1.4156781067946511 5.687324839729078 87.97846181709262 108-109 17.465493061526434 9.049635533729111 18.360775250841996 55.12409615390246 110-111 39.81982775678984 2.459328745122141 22.175841341027432 35.54500215706058 112-113 27.842963678308923 2.7907434232880877 36.32947046515371 33.03682243324928 114-115 27.721800554642627 3.829229472895796 21.517422170854466 46.93154780160711 116-117 18.488118968356336 3.870830423190061 18.45472666744837 59.186323941005234 118-119 18.70661459267744 3.9793053427974283 13.677702488235049 63.63637757629008 120-121 24.430625047899536 4.06341351259621 27.374886409669685 44.13107502983457 122-123 33.411534701857285 1.4565004887585533 31.509997334044254 33.62196747533991 124-125 37.79840974036174 1.6169556734225088 17.37843217876558 43.206202407450164 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 20.0 1 54.0 2 128.5 3 222.5 4 283.0 5 288.5 6 252.5 7 207.0 8 185.5 9 173.5 10 164.0 11 154.5 12 149.0 13 136.0 14 142.5 15 161.0 16 206.0 17 291.5 18 380.5 19 521.5 20 761.0 21 1137.5 22 1730.5 23 2490.0 24 3505.5 25 4874.5 26 6374.5 27 8083.0 28 10012.5 29 12235.0 30 14750.0 31 17358.5 32 20362.0 33 23508.0 34 26562.5 35 29489.5 36 31848.0 37 33947.5 38 36496.5 39 38890.0 40 40900.5 41 42265.0 42 43413.0 43 45003.5 44 46314.5 45 47208.0 46 47649.5 47 47396.0 48 46488.5 49 45631.0 50 44622.5 51 43037.0 52 40769.5 53 37858.5 54 34391.0 55 30829.0 56 27551.0 57 24141.0 58 20647.0 59 17383.5 60 14434.5 61 11808.5 62 9601.0 63 7661.0 64 5933.0 65 4620.5 66 3621.0 67 2794.5 68 2176.0 69 1694.0 70 1275.5 71 919.0 72 713.5 73 599.5 74 456.5 75 321.5 76 241.0 77 181.0 78 119.5 79 82.5 80 61.0 81 46.5 82 36.0 83 33.5 84 29.5 85 18.5 86 12.5 87 9.0 88 6.0 89 7.5 90 11.0 91 8.5 92 5.0 93 4.5 94 3.5 95 3.5 96 3.0 97 3.5 98 4.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 1.7070832860329005E-4 3 0.03687299897831065 4 0.04190889467210771 5 0.04395739461534719 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.828333144131602E-4 12-13 0.0017924374503345455 14-15 8.535416430164503E-5 16-17 3.840937393574026E-4 18-19 0.006657624815528312 20-21 0.0021765311896919486 22-23 6.828333144131602E-4 24-25 0.0 26-27 0.0 28-29 8.962187251672728E-4 30-31 0.0 32-33 6.828333144131602E-4 34-35 4.267708215082251E-5 36-37 8.535416430164503E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.2803124645246753E-4 46-47 0.0 48-49 1.7070832860329005E-4 50-51 8.535416430164503E-5 52-53 0.0 54-55 0.001493697875278788 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.535416430164503E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.267708215082251E-5 80-81 0.0 82-83 5.121249858098701E-4 84-85 0.0 86-87 7.681874787148052E-4 88-89 19.18642117670958 90-91 26.28165679261243 92-93 40.473792430622005 94-95 44.77811758218966 96-97 48.699970723521645 98-99 93.07799066054734 100-101 51.89848999947934 102-103 53.23304503541772 104-105 74.94889419412439 106-107 81.61996229053021 108-109 12.54194090248372 110-111 38.9637919099616 112-113 52.27251194744915 114-115 86.78081648086487 116-117 66.51504921947885 118-119 72.16818355242324 120-121 80.51001673795162 122-123 75.98773972783971 124-125 50.28260763800275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1171589.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.85664071501128 #Duplication Level Percentage of deduplicated Percentage of total 1 78.49077921055886 50.12157487489933 2 13.040781007434504 16.654809348697757 3 3.9675972323279596 7.60072292899919 4 1.6744402138212051 4.276965085309894 5 0.8508940321908116 2.716761725007795 6 0.49606056786926733 1.9006056873187362 7 0.3065733580221121 1.3703721343208775 8 0.21242893308082872 1.0851998445772522 9 0.1516665358010985 0.8716423936627029 >10 0.7384863649861636 8.519389166053456 >50 0.047290276825297156 2.0589565986893774 >100 0.02233359931672112 2.5816661583014078 >500 6.686677652035099E-4 0.24133405416223241 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 2.560624929049351E-4 0.0 0.0 0.0 0.0 16-17 2.987395750557576E-4 0.0 0.0 0.0 0.0 18-19 3.414166572065801E-4 0.0 0.0 4.267708215082251E-5 0.0 20-21 3.414166572065801E-4 0.0 0.0 1.2803124645246753E-4 0.0 22-23 3.414166572065801E-4 0.0 0.0 1.7070832860329005E-4 0.0 24-25 7.681874787148052E-4 0.0 0.0 4.2677082150822514E-4 0.0 26-27 7.681874787148052E-4 0.0 0.0 5.974791501115152E-4 0.0 28-29 9.815728894689178E-4 0.0 0.0 0.001493697875278788 0.0 30-31 0.0011949583002230303 0.0 0.0 0.003286135325613334 0.0 32-33 0.0011949583002230303 0.0 0.0 0.007511166458544763 0.0 34-35 0.0011949583002230303 0.0 0.0 0.013016510056000867 0.0 36-37 0.0011949583002230303 0.0 0.0 0.02172263481476866 0.0 38-39 0.0014510207931279656 0.0 0.0 0.03589142608884174 0.0 40-41 0.0016217291217312556 0.0 0.0 0.055864300535426674 0.0 42-43 0.0016217291217312556 0.0 0.0 0.07528237291405092 0.0 44-45 0.0016217291217312556 0.0 0.0 0.09956563265786894 0.0 46-47 0.0018777916146361907 0.0 0.0 0.12696431939869698 0.0 48-49 0.0019631457789378356 0.0 0.0 0.15858803727245646 0.0 50-51 0.0019631457789378356 0.0 0.0 0.1955890674972196 0.0 52-53 0.0019631457789378356 0.0 0.0 0.2299014415464809 0.0 54-55 0.002048499943239481 0.0 0.0 0.26690247177124404 0.0 56-57 0.002048499943239481 0.0 0.0 0.31324978298703726 0.0 58-59 0.002048499943239481 0.0 0.0 0.36224307329618155 0.0 60-61 0.002048499943239481 0.0 0.0 0.41925965504968044 0.0 62-63 0.002091177025390303 0.0 0.0 0.47951969504664177 0.0 64-65 0.002133854107541126 0.0 0.0 0.5415721724939377 0.0 66-67 0.002219208271842771 0.0 0.0 0.609556764360198 0.0 68-69 0.0023045624361444157 0.0 0.0 0.6892775538179344 0.0 70-71 0.0024325936825968834 0.0 0.0 0.7831244574675932 0.0 72-73 0.002475270764747706 0.0 0.0 0.8799160797856587 0.0 74-75 0.002475270764747706 0.0 0.0 0.9719705459849828 0.0 76-77 0.002475270764747706 0.0 0.0 1.075803886857934 0.0 78-79 0.002475270764747706 0.0 0.0 1.1046535943918898 0.0 80-81 0.002475270764747706 0.0 0.0 1.1052937506241522 0.0 82-83 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 84-85 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 86-87 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 88-89 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 90-91 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 92-93 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 94-95 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 96-97 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 98-99 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 100-101 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 102-103 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 104-105 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 106-107 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 108-109 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 110-111 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 112-113 0.002475270764747706 0.0 0.0 1.1053364277063031 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 575 0.0 1366.0878 108-109 CCGTTCG 65 2.9056537E-8 1169.4796 108-109 CCGCGCC 685 0.0 1109.7252 108-109 CGTCGCC 300 0.0 760.16174 108-109 CGTTGTT 80 5.3330135E-5 739.9804 110-111 CTCCGCC 315 0.0 657.76044 110-111 CCGCTCG 135 5.394777E-7 563.08276 108-109 CCTCGCC 895 0.0 481.29422 108-109 CGACGCC 110 2.2099998E-4 460.7041 108-109 CCGCGCG 330 0.0 460.70404 108-109 TTGCGTG 130 2.285533E-4 455.3726 110-111 TTGTTCG 230 8.141797E-9 440.6735 108-109 CGCCGCC 510 0.0 406.2638 110-111 TTTTTCG 595 0.0 362.15433 92-93 CGGCGCC 215 3.4618333E-6 353.56357 108-109 CGCCTCC 340 1.3460522E-10 348.2261 110-111 GCGCTCG 150 5.59729E-4 337.84967 108-109 CGTCGTT 180 6.059401E-4 328.88022 110-111 CCCCTCG 365 0.0 323.02783 92-93 AATCGCC 160 1.5454134E-8 316.73404 108-109 >>END_MODULE