FastQCFastQC Report
Fri 27 May 2016
SRR939899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939899_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171589
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36610.3124815955083225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25810.22029909806254583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16640.14202932939793733No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56850.043.5984841
GGTATCA35900.040.329271
ATCAACG66150.036.920683
TCAACGC66500.036.7263644
CAACGCA67050.036.336265
TATCAAC68400.035.706192
AACGCAG68800.035.4546556
GTACATG53700.031.3993511
ACGCAGA77650.031.2605487
CGCAGAG78600.030.731338
TACATGG56500.029.4151762
ACATGGG55900.028.6652813
GCAGAGT86350.027.7664649
CATGGGG29550.027.4155624
GAGTACT41200.026.8592312-13
TAGGACG10950.026.6561644
AGAGTAC71450.025.35496110-11
ATTCGCG1651.8189894E-1225.24015280-81
AGTACTT43050.025.0140112-13
GTACTTT44250.024.87505714-15
Produced by FastQC (version 0.11.5)