FastQCFastQC Report
Fri 27 May 2016
SRR939898_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939898_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102187
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT74360.6746586559268073No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40590.3682678166227691No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29410.2668331235988085No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28890.2621152309000197No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA24430.22165022813733062No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22820.2070429065122343No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC21840.1981514933491322No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT19160.17383620020922042No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17460.15841232023241064No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16000.14516592919350346No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA15480.1404480364947146No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14580.13228245297758004No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13580.12320958240298607No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA13220.11994334899613224No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA12770.11586055723756496No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA12040.10923736171811135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG301.3387762E-92524.107108-109
CGTCCGT150.00209742161972.8651110-111
CCGCTCC3050.01572.3945108-109
CTCGTCG200.00372833181479.6489110-111
CGTCGCC900.01402.2817108-109
CCGCGCC2600.01359.1345108-109
CCGCTCG804.1836756E-111262.0536108-109
TCGTGCG200.00512676551262.0536108-109
CGTCGCA200.00512676551262.0536108-109
CCGGTCG200.00512676551262.0536108-109
CCGCGTC200.00512676551262.0536108-109
CTCCGCC1950.01214.0709110-111
CGTCGTC250.0058248671183.7191110-111
CGTCGGT250.0058248671183.7191110-111
CGCCGCC1800.01150.8381110-111
CGACGCC451.5329058E-51121.8253108-109
CCGCTAC451.5329058E-51121.8253108-109
CGTCGCG250.008009521009.64276108-109
CGCGTCT250.008009521009.64276108-109
CCGCACG502.1024422E-51009.64276108-109
Produced by FastQC (version 0.11.5)