FastQCFastQC Report
Fri 27 May 2016
SRR939898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102187
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT80110.7268276617307227No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT41330.3749817408479686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30540.27708546734809975No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28720.2605728429023387No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26450.23997742669801042No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA25310.2296343542429733No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23880.21666014932130392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22050.20005679616979694No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC20660.18744550607111132No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT18230.165398430574848No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC18030.1635838564599292No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17940.16276729810821575No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG15110.13709107438211482No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA14990.13600232991316355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14630.13273609650630971No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA14380.13046787886266126No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13620.12357249722596982No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13160.11939897676165659No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA12920.11722148782375404No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACC250.00204901371.468533
TAGGACC15550.035.236364
GAGTACT42500.026.4577612-13
GTAGGAC53500.026.1605993
AGTACTT43400.025.90909812-13
TAGCCCT9650.025.3040564
TGTAGGA57300.025.2572022
GTCCTAC52850.025.133481
GTACTTT45150.024.5776714-15
CTACAGT55100.024.2119624
TCCTACA56900.023.8647122
GTACATG60900.023.7674051
TACATGG60250.023.6251432
ACATGGG58850.023.2763543
TACAGTG57400.023.2417985
GTGTACG1552.0906166E-423.0575031
CTGTAGG62600.023.0268121
CCTACAG56550.022.853923
CATGGGG20900.022.5120164
GGACCTG36650.022.4019246
Produced by FastQC (version 0.11.5)