##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939898_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1102187 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.76406544443003 34.0 31.0 34.0 31.0 34.0 2 32.868215647616964 34.0 31.0 34.0 31.0 34.0 3 32.910404495788825 34.0 31.0 34.0 31.0 34.0 4 36.3174669997015 37.0 37.0 37.0 35.0 37.0 5 36.229591711751276 37.0 37.0 37.0 35.0 37.0 6 36.37583186882081 37.0 37.0 37.0 35.0 37.0 7 36.37363804871587 37.0 37.0 37.0 35.0 37.0 8 36.39997205555863 37.0 37.0 37.0 35.0 37.0 9 38.224817567254924 39.0 39.0 39.0 37.0 39.0 10-11 38.2107450913502 39.0 39.0 39.0 37.0 39.0 12-13 38.199950643584074 39.0 39.0 39.0 37.0 39.0 14-15 39.71125135752826 41.0 40.0 41.0 37.0 41.0 16-17 39.72434713891563 41.0 40.0 41.0 37.0 41.0 18-19 39.77609879267311 41.0 40.0 41.0 37.5 41.0 20-21 39.793121312445166 41.0 40.0 41.0 37.5 41.0 22-23 39.7405662559983 41.0 40.0 41.0 37.0 41.0 24-25 39.706807465520825 41.0 40.0 41.0 37.0 41.0 26-27 39.6032606082271 41.0 40.0 41.0 37.0 41.0 28-29 39.525048834725865 41.0 40.0 41.0 37.0 41.0 30-31 39.404345179175586 41.0 39.0 41.0 36.0 41.0 32-33 39.252695776669476 41.0 39.0 41.0 35.0 41.0 34-35 39.15671886893966 41.0 39.0 41.0 35.0 41.0 36-37 39.14514869073941 41.0 39.0 41.0 35.0 41.0 38-39 39.19244057496596 41.0 39.0 41.0 35.0 41.0 40-41 39.11828618918568 41.0 39.0 41.0 35.0 41.0 42-43 39.029298567303 41.0 39.0 41.0 35.0 41.0 44-45 38.88726232481421 41.0 39.0 41.0 35.0 41.0 46-47 38.66706012682059 41.0 39.0 41.0 35.0 41.0 48-49 38.43337246764841 41.0 39.0 41.0 35.0 41.0 50-51 38.273231311928015 41.0 39.0 41.0 35.0 41.0 52-53 38.18874383385034 41.0 39.0 41.0 34.5 41.0 54-55 38.087419829847384 41.0 38.5 41.0 34.0 41.0 56-57 37.925512186226115 40.5 38.0 41.0 34.0 41.0 58-59 37.77003630055517 40.0 38.0 41.0 34.0 41.0 60-61 37.56453124560532 40.0 37.0 41.0 33.0 41.0 62-63 37.38025670780004 40.0 37.0 41.0 33.0 41.0 64-65 37.14155220484365 39.5 36.5 41.0 33.0 41.0 66-67 36.85577901027684 39.0 36.0 41.0 32.5 41.0 68-69 36.499640714325245 39.0 35.5 41.0 32.0 41.0 70-71 36.090047333165785 38.5 35.0 40.5 31.5 41.0 72-73 35.73823226004299 37.0 35.0 40.0 31.0 41.0 74-75 35.39275186515537 37.0 35.0 39.0 31.0 41.0 76-77 35.001296513205105 36.0 35.0 39.0 31.0 41.0 78-79 34.609455110611904 36.0 35.0 38.5 30.5 39.5 80-81 34.31413907077474 35.5 35.0 37.0 30.5 39.0 82-83 34.05748434703004 35.0 35.0 37.0 31.0 39.0 84-85 33.77189896088413 35.0 35.0 36.5 31.0 38.0 86-87 33.566324044830864 35.0 35.0 36.0 31.0 37.0 88-89 33.333591305286674 35.0 34.0 36.0 30.0 37.0 90-91 33.1364877284889 35.0 34.0 35.5 30.0 36.5 92-93 32.99372157356238 35.0 34.0 35.0 30.0 36.0 94-95 32.882883757474914 35.0 34.0 35.0 30.0 36.0 96-97 32.755656254337964 35.0 34.0 35.0 29.0 36.0 98-99 32.65606017853595 35.0 34.0 35.0 29.0 36.0 100-101 32.635141314495634 35.0 34.0 35.0 29.0 36.0 102-103 31.756654270101173 34.5 32.5 35.0 27.5 35.0 104-105 32.4446305391009 35.0 34.0 35.0 29.0 35.0 106-107 32.48651181696028 35.0 34.0 35.0 29.0 35.0 108-109 32.50721338575033 35.0 34.0 35.0 29.0 35.0 110-111 32.495795178132205 35.0 34.0 35.0 29.0 35.0 112-113 32.396517560087354 35.0 34.0 35.0 29.0 35.0 114-115 32.32470624313297 35.0 34.0 35.0 29.0 35.0 116-117 32.25642381918858 35.0 34.0 35.0 29.0 35.0 118-119 32.16322230256753 35.0 34.0 35.0 29.0 35.0 120-121 32.05767170180741 35.0 34.0 35.0 29.0 35.0 122-123 31.87136393370635 35.0 34.0 35.0 27.0 35.0 124-125 29.783473675519673 33.5 29.5 34.5 14.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16737413294940495 1101 2 0.19694794050756315 1101 3 0.21871212535989315 1101 4 0.13881515178114512 1101 5 0.13298153929630985 1101 6 0.13903024104825334 1101 7 0.1787438285755769 1101 8 0.15307394900697346 1101 9 0.14574179894587047 1101 10-11 0.1465072309398323 1101 12-13 0.10594948522708592 1101 14-15 0.2841025302604123 1101 16-17 0.32486484727536435 1101 18-19 0.3253884393306308 1101 20-21 0.2991190869804825 1101 22-23 0.3025596562680377 1101 24-25 0.3199063162540554 1101 26-27 0.3066864068924673 1101 28-29 0.3417209049500016 1101 30-31 0.37675827610823376 1101 32-33 0.39384606325451443 1101 34-35 0.3781566257094511 1101 36-37 0.33350689843157966 1101 38-39 0.2524284210045451 1101 40-41 0.28158692306791266 1101 42-43 0.20103118156087163 1101 44-45 0.3519944182092658 1101 46-47 0.27084822480431114 1101 48-49 0.09974229504167909 1101 50-51 0.13920623544914434 1101 52-53 0.18977102394529766 1101 54-55 0.25016123658821954 1101 56-57 0.22265207449082425 1101 58-59 0.21056886020951282 1101 60-61 0.23005900163126825 1101 62-63 0.24952329051269118 1101 64-65 0.2534929784467437 1101 66-67 0.2878865029088189 1101 68-69 0.19405679347443794 1101 70-71 0.3134778906685227 1101 72-73 0.33923528333374264 1101 74-75 0.31795916042137407 1101 76-77 0.35463806919380403 1101 78-79 0.33736330940654824 1101 80-81 0.31943364485185555 1101 82-83 0.3322240152306364 1101 84-85 0.3150111231817263 1101 86-87 0.3364863791687043 1101 88-89 0.29797616224700363 1101 90-91 0.25936674297667395 1101 92-93 0.32462178143092757 1101 94-95 0.40346301330061607 1101 96-97 0.31089252597087835 1101 98-99 0.3171822153551318 1101 100-101 0.44680495819044097 1101 102-103 0.4784605765187706 1101 104-105 0.40182652887073544 1101 106-107 0.43175551019222524 1101 108-109 0.43619663169748435 1101 110-111 0.41212088570661365 1101 112-113 0.3971036618750716 1101 114-115 0.47360819263386844 1101 116-117 0.5748689254757195 1101 118-119 0.5487313427793126 1101 120-121 0.627128456963824 1101 122-123 0.6315441756453737 1101 124-125 0.8123283855260333 1102 1 0.042609086342324076 1102 2 0.09999530199694817 1102 3 0.09321025212604184 1102 4 0.07151139380552252 1102 5 -0.04179181858724945 1102 6 0.06154516937947818 1102 7 3.007224597553204E-4 1102 8 -0.006524936003323489 1102 9 0.07453973263466906 1102 10-11 0.11959707583862667 1102 12-13 0.07183489190423842 1102 14-15 0.1333407698041924 1102 16-17 0.15294129823244873 1102 18-19 0.0731116290706808 1102 20-21 0.10809798273150761 1102 22-23 0.1266770626383149 1102 24-25 0.18250812939098893 1102 26-27 0.16764993956856244 1102 28-29 0.1495697867636494 1102 30-31 0.182017076471368 1102 32-33 0.1564392454504855 1102 34-35 0.14807571862952784 1102 36-37 0.16220829344737098 1102 38-39 0.022281982706182646 1102 40-41 -0.006723266557052909 1102 42-43 0.07752481384442689 1102 44-45 0.0739534500094905 1102 46-47 0.10336321706843421 1102 48-49 0.005775322775761538 1102 50-51 0.040960554116267645 1102 52-53 0.08252678749587972 1102 54-55 0.07373281585861946 1102 56-57 0.11052096730092842 1102 58-59 0.12379274042483956 1102 60-61 0.1129690991928598 1102 62-63 0.09377796081074763 1102 64-65 0.09404113909732814 1102 66-67 0.07263003609638474 1102 68-69 -0.0030786931188444555 1102 70-71 0.05120916791992158 1102 72-73 0.13945936813433235 1102 74-75 -0.011699629712502713 1102 76-77 -0.009780237351641574 1102 78-79 0.008978382226125348 1102 80-81 0.09658754411918835 1102 82-83 0.02631949261757427 1102 84-85 0.053019709369657164 1102 86-87 0.09853133678119264 1102 88-89 0.15535625610049664 1102 90-91 0.14178455723882877 1102 92-93 0.12921088470812947 1102 94-95 0.21789778256679426 1102 96-97 0.22528637818358987 1102 98-99 0.2714294374708004 1102 100-101 0.25824606959989893 1102 102-103 0.10453464917211264 1102 104-105 0.14665675506531528 1102 106-107 0.2010384707967816 1102 108-109 0.213893960581089 1102 110-111 0.14339733794234633 1102 112-113 0.21981054872663464 1102 114-115 0.16965282418859573 1102 116-117 0.19824582907769894 1102 118-119 0.05876880745639568 1102 120-121 0.09220750998976257 1102 122-123 0.1679323825833663 1102 124-125 0.19457118348529434 1103 1 0.0867014230257439 1103 2 0.08826791506227494 1103 3 0.07626301594513762 1103 4 0.0695610169210994 1103 5 -0.10147774823549582 1103 6 0.02934353621364494 1103 7 0.04029758175623499 1103 8 0.0040027021876341 1103 9 0.11243232057437069 1103 10-11 0.06570888488384696 1103 12-13 0.0557403567283572 1103 14-15 0.10471639794489107 1103 16-17 0.04225034345857637 1103 18-19 0.0769668426385195 1103 20-21 0.12290168876165808 1103 22-23 0.12168020334182472 1103 24-25 0.2142606419537998 1103 26-27 0.22250860791027094 1103 28-29 0.20899189731640888 1103 30-31 0.09785878501408263 1103 32-33 0.11876336605349991 1103 34-35 0.1652789221471167 1103 36-37 0.1106379416885801 1103 38-39 0.0343344324549264 1103 40-41 0.018658328920331257 1103 42-43 0.05304931133186841 1103 44-45 0.031183349506981983 1103 46-47 0.04779160902823776 1103 48-49 0.04903380267526103 1103 50-51 0.11666250135245093 1103 52-53 0.18094230257125332 1103 54-55 0.10177144651188286 1103 56-57 0.25326594217530385 1103 58-59 0.18537722534946255 1103 60-61 0.12220276753457426 1103 62-63 0.049382546237886515 1103 64-65 -0.001373433767000165 1103 66-67 -0.0343407553609012 1103 68-69 0.0011345606500086092 1103 70-71 0.06657505987972456 1103 72-73 0.02769743346096476 1103 74-75 -0.08006803423511855 1103 76-77 -0.04024663182400445 1103 78-79 -0.05332689415578784 1103 80-81 -0.08911597346875055 1103 82-83 0.10909901523064036 1103 84-85 0.009557083741519534 1103 86-87 -0.09754749487709091 1103 88-89 -0.09425586701507882 1103 90-91 -0.047362741756145965 1103 92-93 -0.03508559770393305 1103 94-95 -0.07627935311160172 1103 96-97 -0.05335683789680701 1103 98-99 -0.17036390423773895 1103 100-101 -0.07060757361617931 1103 102-103 -0.16854324027512035 1103 104-105 -0.0859374660402139 1103 106-107 -0.10238332568562925 1103 108-109 -0.07315848916766043 1103 110-111 -0.09139851632900786 1103 112-113 -0.14636564474070468 1103 114-115 -0.09998913561040723 1103 116-117 -0.03432954780672759 1103 118-119 -0.08791146892551893 1103 120-121 -0.010391422171039721 1103 122-123 0.037420447910005095 1103 124-125 0.20510510308328378 1104 1 0.06829931824076141 1104 2 0.04653790294840121 1104 3 0.10783630486094609 1104 4 -0.049030367088590765 1104 5 -0.013350855443249543 1104 6 0.04459830605011206 1104 7 -0.028870725330378377 1104 8 -0.019410380792379556 1104 9 -0.12200324877753843 1104 10-11 0.03657578373303494 1104 12-13 0.08267497968414261 1104 14-15 0.06935373895034047 1104 16-17 0.09606469839497578 1104 18-19 0.09044446983415355 1104 20-21 0.16355451280769273 1104 22-23 0.09482488838119707 1104 24-25 0.08888586908886964 1104 26-27 0.12287845951536269 1104 28-29 0.188935870665901 1104 30-31 0.16607653183419302 1104 32-33 0.14390116132909014 1104 34-35 0.020424477870918167 1104 36-37 0.02530329601928827 1104 38-39 -0.008097237744948416 1104 40-41 -0.0761113182631945 1104 42-43 -0.016886712290180128 1104 44-45 -0.08825865533857069 1104 46-47 -0.054189765896055064 1104 48-49 0.007145742408752653 1104 50-51 -0.014781131294434147 1104 52-53 0.0025695360661046607 1104 54-55 -0.004034693706167047 1104 56-57 0.026149800443022286 1104 58-59 0.04028845339307452 1104 60-61 0.0714532218047097 1104 62-63 0.2530430171645932 1104 64-65 0.22652632395559635 1104 66-67 0.14425006081111746 1104 68-69 0.20181464090439505 1104 70-71 0.182496281866392 1104 72-73 0.14291737592008502 1104 74-75 0.09380632221552077 1104 76-77 0.10794934788525978 1104 78-79 0.0013604165649852007 1104 80-81 0.05930619491404343 1104 82-83 0.10259045980967585 1104 84-85 -0.013142605840549493 1104 86-87 0.015222606576571707 1104 88-89 -0.00400810050992817 1104 90-91 0.003534631048218273 1104 92-93 0.043536449540155786 1104 94-95 -0.005324628702148004 1104 96-97 -0.0904252812644657 1104 98-99 -0.07009020647880959 1104 100-101 0.05452227193197956 1104 102-103 0.052403155848441685 1104 104-105 0.07106662239104367 1104 106-107 -0.030262073414284885 1104 108-109 -0.1487083453154483 1104 110-111 -0.15257696878206417 1104 112-113 -0.05154262081580896 1104 114-115 -0.04746566498653948 1104 116-117 -0.14136770649842845 1104 118-119 -0.01963958824713785 1104 120-121 -0.05863585736183197 1104 122-123 0.08322412749813424 1104 124-125 0.14103559975196944 1105 1 0.008859674257273298 1105 2 0.0821041219588281 1105 3 0.04120143959045208 1105 4 0.03919463761075548 1105 5 -0.015307797496575404 1105 6 -0.0013184095991647382 1105 7 -0.09207618671666751 1105 8 0.054051963271376735 1105 9 0.04776175407190664 1105 10-11 0.09195888488385151 1105 12-13 0.08353130500421457 1105 14-15 0.09227181666410189 1105 16-17 0.11665921045364769 1105 18-19 0.08029966283555723 1105 20-21 0.06360519861387104 1105 22-23 0.14331505555857404 1105 24-25 0.05764118382572292 1105 26-27 0.07318902662948545 1105 28-29 0.13374559189769286 1105 30-31 0.0908144500387138 1105 32-33 0.06430523797467913 1105 34-35 0.08840854037372026 1105 36-37 0.03779311410237085 1105 38-39 0.02821528220862035 1105 40-41 0.09884459739324569 1105 42-43 0.0749735724156082 1105 44-45 0.12338162536904917 1105 46-47 0.06478052528439093 1105 48-49 0.04478657361121918 1105 50-51 0.0018549274608190558 1105 52-53 -0.012079557034653021 1105 54-55 -0.06484647220733564 1105 56-57 0.04252086828367396 1105 58-59 0.07738923273862497 1105 60-61 0.14289242270698566 1105 62-63 0.21007681963690317 1105 64-65 0.313774349484234 1105 66-67 0.22834859192973767 1105 68-69 0.15915334143818427 1105 70-71 0.23430135298316657 1105 72-73 0.1214320393723014 1105 74-75 0.15606035246439376 1105 76-77 0.24792608985087128 1105 78-79 0.21171620929249002 1105 80-81 0.05748501175293086 1105 82-83 0.11485948321093531 1105 84-85 0.12434926354448095 1105 86-87 0.05117633517217257 1105 88-89 0.07683557387161954 1105 90-91 0.05216465972169004 1105 92-93 -0.04012254351674471 1105 94-95 0.09675636117410846 1105 96-97 0.09824414732486275 1105 98-99 -0.017041859902768408 1105 100-101 0.17070708155623748 1105 102-103 0.19800848386280734 1105 104-105 0.18507854381200417 1105 106-107 0.2103368097823477 1105 108-109 0.24213553792593956 1105 110-111 0.09801958711926062 1105 112-113 0.018746732106585284 1105 114-115 0.062381554044769416 1105 116-117 -0.0071004837673172005 1105 118-119 0.03613717639468206 1105 120-121 -0.16495576946167745 1105 122-123 -0.02066144864171804 1105 124-125 0.08368268929018186 1106 1 0.05066972446115159 1106 2 0.11456576195222823 1106 3 0.060820432213077424 1106 4 0.03191448582062151 1106 5 8.923741044242206E-5 1106 6 0.05004628740982042 1106 7 0.04811755783278926 1106 8 9.165777857162993E-4 1106 9 0.09863148325379711 1106 10-11 0.13801151646279664 1106 12-13 0.1600450816087431 1106 14-15 -0.020361353251281855 1106 16-17 -0.0178453503213305 1106 18-19 0.0777069742174632 1106 20-21 0.1127985189051941 1106 22-23 0.07018797846144054 1106 24-25 0.12451781898729308 1106 26-27 0.09990992369974805 1106 28-29 0.13118089253172371 1106 30-31 0.04324156013369418 1106 32-33 0.17927772012048138 1106 34-35 0.16616857525716 1106 36-37 0.08318578857853254 1106 38-39 0.19443461188076583 1106 40-41 0.25709582892033467 1106 42-43 0.2021611534371246 1106 44-45 0.2170027274974089 1106 46-47 0.3152975898894823 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2216 64-65 -0.6790535366829502 2216 66-67 -0.7277603880081287 2216 68-69 -0.8351349147645166 2216 70-71 -0.8792931147915226 2216 72-73 -0.8109835985573213 2216 74-75 -0.6699074067336852 2216 76-77 -0.7922386629846656 2216 78-79 -0.7162993069631014 2216 80-81 -0.6463172313303858 2216 82-83 -0.5150458532656472 2216 84-85 -0.5132189668588225 2216 86-87 -0.6161986884162616 2216 88-89 -0.6674757426583042 2216 90-91 -0.6431855333513425 2216 92-93 -0.6278843398422964 2216 94-95 -0.5945162027399604 2216 96-97 -0.6587313376109307 2216 98-99 -0.6994816143578113 2216 100-101 -0.6935145919123471 2216 102-103 -0.7404600498806104 2216 104-105 -0.7465799760459255 2216 106-107 -0.8443842905226795 2216 108-109 -0.7686728902539883 2216 110-111 -0.6319184562947058 2216 112-113 -0.7340530732712907 2216 114-115 -0.8827028291495722 2216 116-117 -0.9207103368290248 2216 118-119 -0.8898507913954994 2216 120-121 -0.9067813235432496 2216 122-123 -1.1363913731305928 2216 124-125 -1.2677200827371813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97.0 3 5.0 4 13.0 5 53.0 6 103.0 7 213.0 8 352.0 9 710.0 10 1513.0 11 2222.0 12 2870.0 13 4728.0 14 7866.0 15 8605.0 16 3283.0 17 2202.0 18 1943.0 19 1867.0 20 1722.0 21 1909.0 22 2003.0 23 2117.0 24 2613.0 25 3347.0 26 4274.0 27 5341.0 28 6996.0 29 8861.0 30 11291.0 31 14563.0 32 18750.0 33 25234.0 34 36746.0 35 60851.0 36 133253.0 37 393721.0 38 326851.0 39 3098.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.86167432541325 19.947677330836076 15.256422820886964 22.93422552286372 2 13.351091965338005 20.80037235060838 43.70120496794101 22.147330716112602 3 19.96403514104231 25.848698995723957 29.182071644829776 25.005194218403954 4 11.564643749200453 17.634666349721055 39.89096224143453 30.909727659643966 5 11.899134750407374 39.09382226687037 35.13398379950479 13.873059183217466 6 29.21400814925235 38.006527023091365 18.998863169317005 13.780601658339284 7 27.267877411001944 32.46000905472483 22.13644327142309 18.135670262850134 8 23.937952452714466 37.51532181018285 20.46204500688177 18.084680730220914 9 25.342432817661614 17.904130605786495 22.254027674069825 34.49940890248207 10-11 23.98150223147252 26.302478617512275 29.701538849578156 20.01448030143705 12-13 27.273729412522556 24.17951763176303 28.799876971875012 19.746875983839406 14-15 20.904937183980575 28.852681078619145 27.159547336341294 23.082834401058985 16-17 19.492145148046834 29.782432168828283 30.54691363065184 20.178509052473043 18-19 20.435506860451085 27.733723950654472 32.12993802322111 19.70083116567334 20-21 21.655496735670273 27.06193085182878 30.19134130358153 21.091231108919416 22-23 20.067928766884656 29.288621500584295 30.618027479985194 20.025422252545855 24-25 21.338983312269153 27.439445393567517 31.794876912901348 19.42669438126198 26-27 19.403377103885276 28.473480452953993 32.14663210507836 19.976510338082377 28-29 19.317411655191 29.336310444597878 30.03369664131404 21.312581258897083 30-31 20.53730446829803 27.288064548030416 31.027493519702194 21.14713746396936 32-33 19.578710327739305 28.64064809329088 31.835886287898514 19.9447552910713 34-35 20.234134498047972 28.662921990551517 31.66912692673748 19.433816584663038 36-37 19.64049657635229 27.64177040738096 31.913096416488308 20.80463659977844 38-39 20.54492567958069 28.859848646373077 30.595760973410137 19.9994647006361 40-41 22.080191473860605 27.17261226996871 31.2820782680253 19.465117988145387 42-43 19.60229979123325 29.244946637911717 30.98439738447287 20.168356186382166 44-45 19.8044433476352 28.714274438003713 30.33242090498255 21.148861309378535 46-47 21.803514285688365 27.59563486050915 30.070668588905512 20.530182264896972 48-49 21.105492988031976 28.178385337515323 29.594070697622094 21.12205097683061 50-51 19.55743444624188 29.772760883588717 30.098749123333878 20.571055546835517 52-53 20.01810944966689 29.422003707174916 29.85813659569565 20.701750247462545 54-55 19.87144649682858 28.07314003885003 30.46792422701411 21.58748923730728 56-57 20.959465136133886 28.06302378815936 28.63125767224618 22.346253403460576 58-59 21.750937000708593 28.469942033429902 28.130798131351575 21.648322834509933 60-61 20.42062735270875 27.465393803410855 31.304624351403167 20.80935449247723 62-63 20.603264237375328 27.656604550770425 30.70413641242366 21.035994799430586 64-65 20.789620998977487 29.053645161846404 29.02828648859041 21.1284473505857 66-67 21.135161274810898 29.38416983687886 28.058986360753664 21.421682527556577 68-69 21.6999021037265 27.470474610932627 30.149874749021716 20.679748536319153 70-71 22.64570349677505 27.522779709795163 29.23337872792911 20.59813806550068 72-73 21.544347737725086 28.402666698119283 29.441056735381565 20.611928828774065 74-75 20.29964062359654 29.796985448022884 30.08178285535939 19.82159107302118 76-77 20.99879603007475 27.57866859253466 30.42764068166291 20.994894695727677 78-79 20.772609366650123 27.750100482041614 29.960796126247182 21.516494025061085 80-81 21.613573740209237 28.286125675588625 29.57447329718097 20.525827287021166 82-83 20.70746615592454 28.37798849015639 30.769687902325103 20.144857451593968 84-85 20.481959957793006 28.325093654706507 30.637087898877414 20.555858488623073 86-87 19.623666401436417 29.96950608199879 28.717676764469186 21.689150752095607 88-89 20.618325202529153 28.33462016880983 29.23328799922336 21.81376662943765 90-91 20.94492861183556 28.30054297012689 29.963885160162352 20.7906432578752 92-93 20.46948476075294 29.613350547593104 30.020450250275132 19.896714441378823 94-95 20.113239268195425 29.29114540780336 29.80322170753493 20.792393616466285 96-97 20.812847547648445 28.54851309260588 29.951904713084076 20.68673464666159 98-99 21.247137470376675 28.436804781830784 29.902456240949686 20.413601506842852 100-101 20.331214213196127 28.234863956842172 30.233435887013727 21.200485942947974 102-103 20.32843925574939 28.80503001998308 29.77138541031091 21.095145313956625 104-105 21.258026833611197 28.03760788449979 29.42587867034126 21.278486611547752 106-107 20.75041477238444 27.730386381401868 29.86111646699673 21.658082379216967 108-109 19.98898551013918 28.835326571912418 29.419167117664962 21.75652080028344 110-111 19.682733534549126 29.53811856730605 29.664827355306777 21.11432054283805 112-113 20.47143998479255 27.871143185479607 29.03347861770098 22.623938212026857 114-115 19.92978123210846 28.561091416869385 28.274236998095503 23.23489035292666 116-117 21.023908311701746 28.358536667244316 27.388494867846948 23.229060153206994 118-119 20.824870972146677 27.31248715618846 29.452328534832418 22.410313336832445 120-121 19.806020795151426 28.30132101834546 29.62560561795714 22.26705256854597 122-123 20.670293742319153 28.286381409097572 28.60820007013415 22.43512477844913 124-125 21.12063373106351 28.380895523471406 28.056943976932796 22.441526768532288 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 146.0 1 100.0 2 152.5 3 207.0 4 107.0 5 66.0 6 160.5 7 417.0 8 725.0 9 793.5 10 607.5 11 449.0 12 436.5 13 486.5 14 543.5 15 644.0 16 787.0 17 927.0 18 1072.5 19 1274.5 20 1558.5 21 1954.0 22 2438.5 23 3050.5 24 3881.0 25 4894.5 26 6253.0 27 7871.0 28 9630.0 29 11832.0 30 14772.5 31 19016.5 32 25037.5 33 32201.5 34 41112.0 35 50688.0 36 57672.5 37 59332.0 38 54836.0 39 47763.5 40 42249.0 41 38940.0 42 36279.0 43 34539.0 44 33908.0 45 33900.5 46 34138.0 47 39448.5 48 50826.0 49 48697.0 50 37604.0 51 31631.5 52 26882.5 53 23960.0 54 21670.0 55 19062.5 56 16009.0 57 13331.5 58 11483.0 59 9659.5 60 7575.5 61 5988.0 62 4792.5 63 3519.5 64 2451.0 65 1867.0 66 1373.0 67 1038.0 68 815.5 69 616.5 70 455.5 71 335.0 72 278.0 73 300.5 74 260.5 75 177.0 76 114.0 77 64.5 78 39.0 79 27.0 80 15.5 81 11.0 82 9.0 83 2.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 2.0 98 5.5 99 5.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0162404383285232 2 0.0 3 0.0 4 0.0 5 0.1118684941847436 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.8145741149187933E-4 18-19 0.0 20-21 0.004218884817186195 22-23 2.72186117237819E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 7.711939988404872E-4 92-93 0.0 94-95 6.804652930945474E-4 96-97 0.0 98-99 0.0013609305861890949 100-101 0.0 102-103 4.0827917585672854E-4 104-105 0.0022228532907755216 106-107 0.006940745989564384 108-109 2.2682176436484918E-4 110-111 0.004853985757407772 112-113 0.008664591398737239 114-115 0.0054437223447563795 116-117 0.0016331167034269142 118-119 2.2682176436484918E-4 120-121 6.351009402215777E-4 122-123 0.0012248375275701856 124-125 0.0014516592919350346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1102187.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.6126141912368 #Duplication Level Percentage of deduplicated Percentage of total 1 82.43662649345087 55.737558223287834 2 11.830894194759315 15.998353694552096 3 3.024732141708965 6.13530141987605 4 1.1190964275926722 3.026601400064589 5 0.5343267072320596 1.8063612754077591 6 0.26308653136645677 1.0672780886514575 7 0.16755890739703727 0.793036703209874 8 0.11786372797696583 0.6375259813477979 9 0.0813048082160301 0.494750756682265 >10 0.3666620976788601 4.378721354716993 >50 0.029541613404065612 1.3893982255914612 >100 0.02307437406404419 3.2371542236309447 >500 0.00254891097175935 1.1026038005381888 >1k 0.00254891097175935 3.4687211698076195 >5k 1.3415320903996576E-4 0.7266336826350306 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8011 0.7268276617307227 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4133 0.3749817408479686 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3054 0.27708546734809975 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2872 0.2605728429023387 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2645 0.23997742669801042 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 2531 0.2296343542429733 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2388 0.21666014932130392 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2205 0.20005679616979694 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 2066 0.18744550607111132 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1823 0.165398430574848 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1803 0.1635838564599292 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1794 0.16276729810821575 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1511 0.13709107438211482 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 1499 0.13600232991316355 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1463 0.13273609650630971 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1438 0.13046787886266126 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1362 0.12357249722596982 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1316 0.11939897676165659 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 1292 0.11722148782375404 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.360930586189095E-4 0.0 18-19 0.0 0.0 0.0 2.2682176436484918E-4 0.0 20-21 0.0 0.0 0.0 3.1755047011078883E-4 0.0 22-23 0.0 0.0 0.0 4.990078816026681E-4 0.0 24-25 0.0 0.0 0.0 7.711939988404872E-4 0.0 26-27 0.0 0.0 0.0 0.0013609305861890949 0.0 28-29 0.0 0.0 0.0 0.0023135819965214616 0.0 30-31 0.0 0.0 0.0 0.004445706581551043 0.0 32-33 0.0 0.0 0.0 0.008982141868848027 0.0 34-35 0.0 0.0 0.0 0.01592288785841241 0.0 36-37 0.0 0.0 0.0 0.02476893666864153 0.0 38-39 9.072870574593966E-5 0.0 0.0 0.040283545351197214 0.0 40-41 9.072870574593966E-5 0.0 0.0 0.060425318026795814 0.0 42-43 9.072870574593966E-5 0.0 0.0 0.08183729258283758 0.0 44-45 9.072870574593966E-5 0.0 0.0 0.10851153207214384 0.0 46-47 9.072870574593966E-5 0.0 0.0 0.13718180308786077 0.0 48-49 9.072870574593966E-5 0.0 0.0 0.1703885093908747 0.0 50-51 9.072870574593966E-5 0.0 0.0 0.2079955579225667 0.0 52-53 9.072870574593966E-5 0.0 0.0 0.2456933351600046 0.0 54-55 9.072870574593966E-5 0.0 0.0 0.2835725698089344 0.0 56-57 9.072870574593966E-5 0.0 0.0 0.3300256671508555 0.0 58-59 9.072870574593966E-5 0.0 0.0 0.37956354048813856 0.0 60-61 9.072870574593966E-5 0.0 0.0 0.4397166723976966 0.0 62-63 9.072870574593966E-5 0.0 0.0 0.500686362658968 0.0 64-65 9.072870574593966E-5 0.0 0.0 0.5596600213938288 0.0 66-67 9.072870574593966E-5 0.0 0.0 0.6341936531641182 0.0 68-69 9.072870574593966E-5 0.0 0.0 0.7295041585502279 0.0 70-71 9.072870574593966E-5 0.0 0.0 0.8337060770994396 0.0 72-73 9.072870574593966E-5 0.0 0.0 0.9288351250740573 0.0 74-75 9.072870574593966E-5 0.0 0.0 1.028500608335972 0.0 76-77 9.072870574593966E-5 0.0 0.0 1.1412310252253022 0.0 78-79 9.072870574593966E-5 0.0 0.0 1.275146594906309 0.0 80-81 9.072870574593966E-5 0.0 0.0 1.4200403379825746 0.0 82-83 9.072870574593966E-5 0.0 0.0 1.561123475417511 0.0 84-85 9.072870574593966E-5 0.0 0.0 1.706516226375379 0.0 86-87 9.072870574593966E-5 0.0 0.0 1.8607550261434764 0.0 88-89 9.072870574593966E-5 0.0 0.0 2.0273329298930216 0.0 90-91 9.072870574593966E-5 0.0 0.0 2.212192667850374 0.0 92-93 9.072870574593966E-5 0.0 0.0 2.414154766840835 0.0 94-95 9.072870574593966E-5 0.0 0.0 2.6075430031383062 0.0 96-97 9.072870574593966E-5 0.0 0.0 2.8023375343748382 0.0 98-99 9.072870574593966E-5 0.0 0.0 3.0220824596915046 0.0 100-101 1.8145741149187933E-4 0.0 0.0 3.279434433539862 0.0 102-103 2.72186117237819E-4 0.0 0.0 3.565229856639572 0.0 104-105 3.6291482298375866E-4 0.0 0.0 3.8305205922407 0.0 106-107 4.082791758567285E-4 0.0 0.0 4.095902056547573 0.0 108-109 4.536435287296983E-4 0.0 0.0 4.3868236515219285 0.0 110-111 4.536435287296983E-4 0.0 0.0 4.6973426469374076 0.0 112-113 4.536435287296983E-4 0.0 0.0 5.0531806308729825 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAACC 25 0.002049013 71.46853 3 TAGGACC 1555 0.0 35.23636 4 GAGTACT 4250 0.0 26.45776 12-13 GTAGGAC 5350 0.0 26.160599 3 AGTACTT 4340 0.0 25.909098 12-13 TAGCCCT 965 0.0 25.304056 4 TGTAGGA 5730 0.0 25.257202 2 GTCCTAC 5285 0.0 25.13348 1 GTACTTT 4515 0.0 24.57767 14-15 CTACAGT 5510 0.0 24.211962 4 TCCTACA 5690 0.0 23.864712 2 GTACATG 6090 0.0 23.767405 1 TACATGG 6025 0.0 23.625143 2 ACATGGG 5885 0.0 23.276354 3 TACAGTG 5740 0.0 23.241798 5 GTGTACG 155 2.0906166E-4 23.057503 1 CTGTAGG 6260 0.0 23.026812 1 CCTACAG 5655 0.0 22.85392 3 CATGGGG 2090 0.0 22.512016 4 GGACCTG 3665 0.0 22.401924 6 >>END_MODULE