##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939896_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 341851 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.121497377512426 31.0 31.0 33.0 30.0 34.0 2 31.226967304468907 31.0 31.0 34.0 28.0 34.0 3 31.19166537468078 31.0 31.0 34.0 28.0 34.0 4 34.987629113268646 35.0 35.0 37.0 32.0 37.0 5 34.78428613635765 35.0 35.0 37.0 32.0 37.0 6 34.954000427086655 35.0 35.0 37.0 32.0 37.0 7 34.84742475522962 35.0 35.0 37.0 32.0 37.0 8 34.91370801899073 36.0 35.0 37.0 32.0 37.0 9 36.23629007959608 38.0 35.0 39.0 32.0 39.0 10-11 36.10755124308544 37.5 35.0 39.0 30.5 39.0 12-13 36.08385524687657 37.5 35.0 39.0 31.0 39.0 14-15 36.48454151077516 38.0 35.0 40.0 30.0 41.0 16-17 36.533039248093466 38.0 35.0 40.0 30.5 41.0 18-19 36.855113777639964 38.5 35.5 40.0 31.0 41.0 20-21 36.86379738541061 38.5 35.0 40.0 31.0 41.0 22-23 36.667428207025864 38.0 35.0 40.0 31.0 41.0 24-25 36.241011434806396 38.0 34.5 40.0 30.0 41.0 26-27 35.693521446478144 38.0 34.5 40.0 28.5 41.0 28-29 35.38805210457188 38.0 34.0 40.0 28.0 41.0 30-31 34.79732398033061 37.0 33.5 39.5 27.0 41.0 32-33 34.198783973134496 35.5 33.0 39.0 25.5 41.0 34-35 33.62261043554063 35.0 33.0 39.0 24.0 40.5 36-37 33.0465860857508 35.0 33.0 39.0 21.5 41.0 38-39 32.478009425158916 35.0 32.0 39.0 18.0 40.5 40-41 32.268559986660854 35.0 32.5 39.0 13.5 41.0 42-43 31.51909603891754 35.0 31.0 39.0 4.5 41.0 44-45 30.715889378705924 35.0 30.0 39.0 2.0 41.0 46-47 29.787930999178005 35.0 28.0 39.0 2.0 40.0 48-49 28.907785848220424 35.0 25.0 39.0 2.0 40.0 50-51 28.247504029533335 35.0 23.0 38.0 2.0 40.0 52-53 27.998224372606778 35.0 22.5 38.0 2.0 40.0 54-55 27.6297319592454 35.0 21.0 38.5 2.0 40.0 56-57 26.81384287306458 34.0 14.0 38.0 2.0 40.0 58-59 26.16241578933512 33.0 5.0 38.0 2.0 40.0 60-61 25.23361493750201 33.0 2.0 37.0 2.0 40.0 62-63 24.764274493858437 33.0 2.0 37.0 2.0 40.0 64-65 24.11689888284662 32.5 2.0 36.5 2.0 39.5 66-67 22.859680094544117 30.5 2.0 36.0 2.0 39.0 68-69 21.216799131785486 28.0 2.0 35.0 2.0 37.5 70-71 20.28010887784444 26.0 2.0 34.0 2.0 36.5 72-73 20.48132958511164 27.0 2.0 34.0 2.0 36.0 74-75 20.467006093297957 28.5 2.0 34.0 2.0 35.5 76-77 20.05728665412709 28.0 2.0 33.5 2.0 35.0 78-79 20.30384143969156 29.0 2.0 34.0 2.0 35.0 80-81 20.002268532196776 29.0 2.0 34.0 2.0 35.0 82-83 19.559315315736974 27.0 2.0 34.0 2.0 35.0 84-85 19.232607188511956 26.0 2.0 34.0 2.0 35.0 86-87 19.162183231875876 26.0 2.0 34.0 2.0 35.0 88-89 18.71193882714984 25.5 2.0 34.0 2.0 35.0 90-91 18.235914184834915 24.5 2.0 33.0 2.0 35.0 92-93 17.85230553662268 23.5 2.0 33.0 2.0 35.0 94-95 17.20640278951941 19.5 2.0 33.0 2.0 35.0 96-97 16.682270930902646 16.5 2.0 33.0 2.0 35.0 98-99 16.265704941626616 2.0 2.0 33.0 2.0 35.0 100-101 15.226294496725181 2.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2730693956908219 1101 2 0.23957752683316258 1101 3 0.38722951523391913 1101 4 0.4055093957752547 1101 5 0.3848542434765818 1101 6 0.1731598324611241 1101 7 0.26885069278517904 1101 8 0.13722166325987217 1101 9 0.249966104614046 1101 10-11 0.31651863657290846 1101 12-13 0.51307529904836 1101 14-15 0.3182956508624315 1101 16-17 0.6228753412224677 1101 18-19 0.36302121833614365 1101 20-21 0.3482550984483126 1101 22-23 0.1308125918536831 1101 24-25 0.015221962652574916 1101 26-27 -0.04119457929689929 1101 28-29 -0.20556831462767633 1101 30-31 -0.49571015778601435 1101 32-33 0.11179289559458994 1101 34-35 -0.48598284767432887 1101 36-37 -0.5035885138458696 1101 38-39 -0.1381655602688241 1101 40-41 -0.1908559179345204 1101 42-43 -0.08543885266142937 1101 44-45 -0.7733382153267421 1101 46-47 -0.1411997940031675 1101 48-49 -0.44575785852451943 1101 50-51 -0.8653609653907033 1101 52-53 -1.2102168454237834 1101 54-55 -0.96832996527991 1101 56-57 -1.0278206538967254 1101 58-59 -0.33977954798684706 1101 60-61 -0.29353254142666785 1101 62-63 -0.4051046810263337 1101 64-65 -0.8612892902080453 1101 66-67 -0.5398577575023857 1101 68-69 -7.059059264253051 1101 70-71 -6.032357872520764 1101 72-73 -2.134435471432049 1101 74-75 -1.2316811727184955 1101 76-77 -1.8088414137038846 1101 78-79 -2.1374837999754384 1101 80-81 -2.108718157718389 1101 82-83 -2.0086120775832335 1101 84-85 -2.3459356735154664 1101 86-87 -2.6233172191375616 1101 88-89 -2.284762901917354 1101 90-91 -2.52892028594529 1101 92-93 -2.794822465775752 1101 94-95 -2.148182053484586 1101 96-97 -2.7574529633576432 1101 98-99 -2.7765150686912854 1101 100-101 -2.9232860939713863 1102 1 0.03698713561603739 1102 2 0.11162685266312877 1102 3 0.11366189050302111 1102 4 0.2149761027284569 1102 5 0.42242458754879664 1102 6 0.14944113321271857 1102 7 0.12415907364403722 1102 8 0.20644211874954266 1102 9 0.23850332559829468 1102 10-11 0.14215503797790063 1102 12-13 0.14260281349486092 1102 14-15 0.287674734595484 1102 16-17 0.10140387542195839 1102 18-19 0.40585084404902005 1102 20-21 0.40820335542606756 1102 22-23 0.21526324713939715 1102 24-25 0.6563161198456058 1102 26-27 0.011988371943800757 1102 28-29 0.042170288688403446 1102 30-31 0.19041364778108516 1102 32-33 0.32044568858474065 1102 34-35 -0.2570246953156783 1102 36-37 -0.17382588968149548 1102 38-39 0.12384486387288263 1102 40-41 -0.030872724974592813 1102 42-43 -0.10415792959612702 1102 44-45 -0.3267572205010403 1102 46-47 -0.29547992392724964 1102 48-49 -0.7231401148224386 1102 50-51 -0.3163089579880527 1102 52-53 -0.17328384697229282 1102 54-55 -0.2207141100093004 1102 56-57 -0.13380414900568383 1102 58-59 -0.20276912384514034 1102 60-61 0.042249458044569366 1102 62-63 -0.4987897661945908 1102 64-65 -0.5943642231687285 1102 66-67 0.0966569533787549 1102 68-69 -3.1200603217746767 1102 70-71 0.4064002949453389 1102 72-73 0.1448777060427382 1102 74-75 0.03843534350312794 1102 76-77 -0.06466250370652915 1102 78-79 -0.10504054734107271 1102 80-81 0.286875187449116 1102 82-83 0.425500548469568 1102 84-85 0.2273354674748269 1102 86-87 0.15127960074384106 1102 88-89 0.47593506235352123 1102 90-91 0.09001312796111804 1102 92-93 0.11180876132701556 1102 94-95 0.31278878913353836 1102 96-97 -0.5223621298039909 1102 98-99 -0.11727308508286782 1102 100-101 -0.34830120142313703 1103 1 0.3562459892122405 1103 2 0.3199328037401621 1103 3 0.4580759102182448 1103 4 0.28293886285989345 1103 5 0.3662544524629965 1103 6 0.3319895085321818 1103 7 0.32423209299416555 1103 8 0.39521539366739233 1103 9 0.5203945267666086 1103 10-11 0.5392981559041488 1103 12-13 0.38825816216225917 1103 14-15 0.38208875431070766 1103 16-17 0.6555294687041808 1103 18-19 0.5000640605513951 1103 20-21 0.34637422070536417 1103 22-23 0.5678554340689388 1103 24-25 0.403048503927387 1103 26-27 0.40885766730707473 1103 28-29 0.20404506050294202 1103 30-31 0.4907057251815914 1103 32-33 0.6223989562006551 1103 34-35 0.7576430666412435 1103 36-37 0.333193095349543 1103 38-39 0.18079995697255669 1103 40-41 0.12377313117728761 1103 42-43 0.568322902824459 1103 44-45 0.3091591723430618 1103 46-47 -0.09083406777537206 1103 48-49 -0.04272755549421703 1103 50-51 0.45961291130731396 1103 52-53 0.6107340427684882 1103 54-55 0.124512249976096 1103 56-57 0.2010717180990973 1103 58-59 -0.33142009135153216 1103 60-61 -0.5390393334851815 1103 62-63 -1.0679573456031335 1103 64-65 -0.6982798858193284 1103 66-67 -0.6490531598012375 1103 68-69 -2.7938555024975678 1103 70-71 0.0649253040727551 1103 72-73 0.1306115505115244 1103 74-75 -0.005348884317239566 1103 76-77 -0.2643795537247833 1103 78-79 -0.3879540194111719 1103 80-81 -0.23186340327742627 1103 82-83 -0.2103884621182388 1103 84-85 -0.7072209399731477 1103 86-87 -0.5795345650100785 1103 88-89 -0.3207608120531944 1103 90-91 -0.08921286692752517 1103 92-93 -0.04889770088547252 1103 94-95 -0.1642740075075757 1103 96-97 -0.49543624444437384 1103 98-99 -0.012983198262862317 1103 100-101 -0.02297261434756237 1104 1 0.1006556025873735 1104 2 0.11183458012469671 1104 3 -0.024996190095233572 1104 4 0.04709769253596363 1104 5 0.17612906061346933 1104 6 0.505970699754748 1104 7 0.23854766248214787 1104 8 0.3950586538554788 1104 9 0.7465700753559474 1104 10-11 0.20405782152588614 1104 12-13 0.2474013178571397 1104 14-15 -0.21017874830162242 1104 16-17 -0.10518108510982671 1104 18-19 0.06181954644480925 1104 20-21 0.2608726533072456 1104 22-23 0.46140297847855294 1104 24-25 0.09489803370795613 1104 26-27 0.07165808527990691 1104 28-29 0.2566835140034627 1104 30-31 0.11061168129445065 1104 32-33 0.20505308368237252 1104 34-35 0.07423136340195668 1104 36-37 0.6852829595083705 1104 38-39 0.7506797897834261 1104 40-41 0.4180782513444754 1104 42-43 0.38765414629363804 1104 44-45 0.4841069257390913 1104 46-47 0.2813446156102124 1104 48-49 0.0030404695841532714 1104 50-51 0.16807686115057052 1104 52-53 -0.011810366844887454 1104 54-55 -0.3095002892088523 1104 56-57 -0.0779773937086361 1104 58-59 0.2272267216056285 1104 60-61 -0.10688154874118894 1104 62-63 0.45696428449091187 1104 64-65 0.018674137169174543 1104 66-67 -0.11979506157762287 1104 68-69 -3.435234812842392 1104 70-71 -0.2746514984350803 1104 72-73 -0.009827320961832697 1104 74-75 -0.29607511211243676 1104 76-77 -0.29813085988988064 1104 78-79 -0.6585130580736624 1104 80-81 -0.7793033196828603 1104 82-83 -1.0824104056919062 1104 84-85 -0.5133860392416949 1104 86-87 -0.3649577625022431 1104 88-89 -0.01895830421620559 1104 90-91 -0.048904611964097455 1104 92-93 -0.15467095062424008 1104 94-95 0.22642609698563732 1104 96-97 -0.3573745934516843 1104 98-99 -0.3480929161312005 1104 100-101 -0.45998933326083247 1105 1 0.008572066890430108 1105 2 -0.041953666597013495 1105 3 0.058588074110691224 1105 4 -0.1678934034653281 1105 5 0.12007434149415985 1105 6 -0.03582952689249197 1105 7 -0.16394545715521502 1105 8 0.10392217983896757 1105 9 -0.1186152897079964 1105 10-11 -0.10406505365624241 1105 12-13 -0.2841916884566018 1105 14-15 0.02415021397779782 1105 16-17 -0.10326267811613121 1105 18-19 -0.09496368165944347 1105 20-21 -0.01815842974277615 1105 22-23 0.1368481784137927 1105 24-25 -0.027062464915978524 1105 26-27 -0.36229043341849376 1105 28-29 -0.1918149480331266 1105 30-31 -0.19299039090888215 1105 32-33 -0.1476181159290917 1105 34-35 -0.09833217877062594 1105 36-37 -0.07069081416817369 1105 38-39 0.04526858693414937 1105 40-41 -0.25518546890807414 1105 42-43 -0.6578614761768158 1105 44-45 -0.5571873918908246 1105 46-47 -0.21063773828326404 1105 48-49 -0.01656451665512293 1105 50-51 0.16221641109391172 1105 52-53 -0.0010243865955779086 1105 54-55 -0.1957395639376891 1105 56-57 -0.2728292635483669 1105 58-59 -0.1338101895162751 1105 60-61 0.20912541256688755 1105 62-63 0.5666777376234968 1105 64-65 0.7327060296736327 1105 66-67 1.1979805576550149 1105 68-69 1.9089613989108862 1105 70-71 2.2666751973719457 1105 72-73 1.605835622214471 1105 74-75 0.9593118071040116 1105 76-77 0.6027352563477706 1105 78-79 0.9630062878871612 1105 80-81 0.5956440657793358 1105 82-83 0.7542295489786461 1105 84-85 0.567875090756683 1105 86-87 0.6398423022541095 1105 88-89 0.6399092690394212 1105 90-91 0.7510346789538218 1105 92-93 0.40020601230274977 1105 94-95 0.8725164320997685 1105 96-97 0.360923123886824 1105 98-99 0.22841671637648986 1105 100-101 0.3464255930788145 1106 1 0.2581366911029441 1106 2 0.40781159161895175 1106 3 0.60301245515479 1106 4 0.419924099845133 1106 5 0.35858804479659057 1106 6 0.6785318550745245 1106 7 0.5109712797333543 1106 8 0.3323560308080289 1106 9 0.5013839077559865 1106 10-11 0.3820590986650956 1106 12-13 0.4819774058814943 1106 14-15 0.9963985186204738 1106 16-17 1.0332165963913056 1106 18-19 0.6704991809865106 1106 20-21 0.7667057410368869 1106 22-23 0.505501121714623 1106 24-25 0.7624243133031996 1106 26-27 0.8782697367191403 1106 28-29 0.5101833666412432 1106 30-31 0.6835314180072842 1106 32-33 0.7324870162887152 1106 34-35 0.733050992049165 1106 36-37 0.584708246864686 1106 38-39 0.28822031439291607 1106 40-41 0.3193701267742881 1106 42-43 0.292630077131637 1106 44-45 0.2983200115039004 1106 46-47 0.9215720446307429 1106 48-49 0.6840012712346102 1106 50-51 0.9087193604137589 1106 52-53 0.6475250295594712 1106 54-55 1.267078942542792 1106 56-57 1.1686578356852166 1106 58-59 0.9640992041677663 1106 60-61 0.6878060433601938 1106 62-63 0.7293879017421112 1106 64-65 0.6136859261464842 1106 66-67 1.1274296166815425 1106 68-69 1.6309308222887573 1106 70-71 1.5218535610010129 1106 72-73 1.0842634541634268 1106 74-75 0.6780750991067421 1106 76-77 0.3430667037214725 1106 78-79 0.31642308496592975 1106 80-81 0.2647825433685185 1106 82-83 0.4013183495885748 1106 84-85 0.446948964196757 1106 86-87 0.7740784886029743 1106 88-89 0.9984181571257764 1106 90-91 0.6708492931346335 1106 92-93 0.37623827425050393 1106 94-95 0.6941046508710862 1106 96-97 0.2720462730381463 1106 98-99 0.5922745069948441 1106 100-101 0.5344477430727963 1107 1 0.26039049837067907 1107 2 0.14225913108041865 1107 3 0.07504223080573524 1107 4 0.2629591211531306 1107 5 0.07071971793104836 1107 6 0.10416811915090562 1107 7 0.12325294215429494 1107 8 -0.17513152960432166 1107 9 0.0913576397909992 1107 10-11 -0.10581875132974972 1107 12-13 -0.101532502140941 1107 14-15 -0.07824297524107493 1107 16-17 -0.17124102171533195 1107 18-19 0.021533630906759527 1107 20-21 0.2548573809991126 1107 22-23 -0.11774007534273068 1107 24-25 -0.3198475879102105 1107 26-27 0.08913959522131165 1107 28-29 0.2847828000150514 1107 30-31 0.02522923324270465 1107 32-33 -0.027609484754862024 1107 34-35 0.017780654216160485 1107 36-37 -0.45800298804710593 1107 38-39 -0.2591215421411448 1107 40-41 -0.6372718591156143 1107 42-43 -0.1623810728655819 1107 44-45 0.04648085715778549 1107 46-47 -0.0502077488760726 1107 48-49 0.3360856461602104 1107 50-51 -0.5634731020293984 1107 52-53 -0.7256506959839406 1107 54-55 -1.674706961638659 1107 56-57 -1.2253695750552893 1107 58-59 -1.530373412867526 1107 60-61 -1.0527601066767076 1107 62-63 -1.4517380495788252 1107 64-65 -1.2289205543430057 1107 66-67 -1.0700880209475194 1107 68-69 -0.2328527169822472 1107 70-71 -9.225899088427525E-4 1107 72-73 -0.9719587204431477 1107 74-75 -1.230899656664672 1107 76-77 -1.7116278022265163 1107 78-79 -1.5649861794516902 1107 80-81 -1.3510623565989412 1107 82-83 -1.384010476130225 1107 84-85 -1.4712751309075607 1107 86-87 -1.327057144566087 1107 88-89 -1.1521147413179875 1107 90-91 -0.9560821165265807 1107 92-93 -1.0025441936684523 1107 94-95 -1.2176714921028307 1107 96-97 -1.2402028520243498 1107 98-99 -0.7429317084342131 1107 100-101 -0.5560696009264419 1108 1 0.08086720133345437 1108 2 0.07069037949773715 1108 3 0.1944395465818829 1108 4 0.13748431740534528 1108 5 0.10811051306905739 1108 6 0.23634556913824412 1108 7 0.19733815360022788 1108 8 -0.009330060878063762 1108 9 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98-99 0.042583611900049334 2316 100-101 -0.760804976971011 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 32.0 5 87.0 6 260.0 7 505.0 8 1008.0 9 1810.0 10 3034.0 11 4398.0 12 5764.0 13 6826.0 14 8464.0 15 10304.0 16 11989.0 17 12983.0 18 11303.0 19 8702.0 20 8578.0 21 8473.0 22 8079.0 23 7967.0 24 7812.0 25 7415.0 26 7138.0 27 7875.0 28 8926.0 29 10087.0 30 11830.0 31 13586.0 32 15638.0 33 18181.0 34 22481.0 35 28638.0 36 29349.0 37 24383.0 38 7793.0 39 140.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.504290658856775 20.7692465234399 14.978831969431432 12.747630848271887 2 15.136487316773458 28.371807250065828 32.12703706954563 24.364668363615085 3 28.539041863267915 30.351819944946772 31.496763209702472 9.612374982082837 4 10.913819178530998 21.008275535247815 36.890926163738 31.186979122483187 5 8.006119625216835 45.204489675326386 31.069676554990334 15.71971414446645 6 21.9899897908738 38.54310796224086 13.269816381990985 26.197085864894355 7 17.35434443661127 42.219856019142846 19.817113303749295 20.608686240496592 8 24.849130176597406 24.558652746371955 18.54462909279189 32.047587984238746 9 20.954450915749845 23.47572480408131 24.648457953903893 30.921366326264955 10-11 25.024937765283706 26.977835372720865 21.096618117249914 26.90060874474552 12-13 32.98030428461522 27.37230547811766 22.473972578696568 17.173417658570546 14-15 25.18231627229407 28.591550119789034 31.449520405088766 14.776613202828132 16-17 15.634488810881967 33.10779581687875 29.985373701916046 21.272341670323243 18-19 14.643368017060066 33.38150246744927 33.09380402573051 18.881325489760158 20-21 20.249465410368845 25.672588349895133 30.779199124764883 23.298747114971142 22-23 16.811417839936112 28.419837882586275 34.834913456447396 19.933830821030217 24-25 22.9258111367396 29.473702685823188 31.507632722491262 16.09285345494595 26-27 19.449848033207452 25.406683028570924 40.49322658117133 14.650242357050294 28-29 15.214669549014046 35.941682194874375 33.713957250381014 15.129691005730567 30-31 16.12066695919263 30.314319145824193 33.96913851104286 19.595875383940324 32-33 17.52416278579954 31.09027989047764 36.12307224263415 15.26248508108867 34-35 21.150006655131108 28.46172860814149 33.874032263490534 16.514232473236863 36-37 15.664026303115296 28.995349232118254 39.91023447072447 15.430389994041981 38-39 15.51157289489247 30.9744008618923 37.044242243262076 16.469783999953158 40-41 14.44164026771755 32.49105558711068 36.02475743220691 17.04254671296486 42-43 14.174812142895831 32.19796837647252 31.542133717074684 22.085085763556968 44-45 15.781669245418628 27.878772797070027 33.75496049898484 22.584597458526495 46-47 20.064375797744653 31.045879831844207 33.21764816094242 15.672096209468716 48-49 17.157436045311698 29.22747918188352 33.09674331324587 20.518341459558915 50-51 9.953379441577926 31.362096095436748 38.07896951732719 20.60555494565814 52-53 15.040020372411188 33.62911381474863 29.400521311269074 21.930344501571106 54-55 12.631706803095403 28.170475432291 31.72670545675919 27.471112307854405 56-57 18.369926686495887 26.92014519321788 28.71048115370379 25.999446966582447 58-59 21.570070216500877 27.751462843768287 29.002779403159746 21.675687536571093 60-61 17.570168920302617 30.765202534682203 32.17882030788314 19.48580823713204 62-63 14.448005171911953 31.60234198682742 31.16471866758911 22.784934173671516 64-65 14.21703065646881 35.62375580138458 28.07482524540241 22.084388296744205 66-67 17.544949119178877 29.41245393777132 25.992717920477116 27.049879022572686 68-69 16.039324564198264 28.44365226302707 29.528953855595574 25.988069317179097 70-71 20.401958409489623 28.169239464740553 27.113107690500833 24.315694435268988 72-73 18.381943589833746 24.432147817373114 33.72230328815128 23.463605304641856 74-75 15.10179836958748 31.898529351521855 28.50661586989472 24.493056408995944 76-77 16.41382478635605 26.600409365366634 28.63377600779521 28.351989840482112 78-79 17.423362596793208 26.93652237762442 30.512934527274695 25.127180498307673 80-81 19.9675001755166 25.350301888558658 28.760004446420634 25.92219348950411 82-83 16.865242459434082 24.780094251589144 32.87631160944388 25.478351679532896 84-85 16.989590478878924 26.869640383734993 30.144609997645173 25.99615913974091 86-87 16.80205822470806 27.9486789450282 29.279533593877982 25.969729236385763 88-89 16.349959485272826 27.800415970700687 25.82499393010405 30.024630613922437 90-91 18.5122129589001 24.00994588269709 27.636243966652042 29.84159719175077 92-93 20.47977954888846 27.083805648474673 27.71084513572692 24.725569666909955 94-95 19.126155755688945 25.196462000758185 28.426210936299327 27.251171307253543 96-97 14.535467165991312 27.037405462338537 31.219444403809298 27.20768296786085 98-99 18.13828970178403 28.514332945659888 25.013714862522217 28.333662490033866 100-101 16.55005756096562 25.928472271997872 27.028640054532982 30.492830112503526 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3188.0 1 2037.0 2 981.0 3 1108.0 4 960.5 5 868.5 6 1322.0 7 2279.5 8 3063.0 9 3310.0 10 2719.5 11 1871.0 12 1581.5 13 1490.0 14 1442.5 15 1334.0 16 1251.5 17 1243.5 18 1274.5 19 1284.5 20 1358.5 21 1515.0 22 1685.0 23 1865.0 24 2060.5 25 2319.5 26 2639.5 27 2909.5 28 3230.0 29 3686.0 30 4137.5 31 4544.5 32 4957.5 33 5414.5 34 5716.0 35 5957.0 36 6192.0 37 6277.5 38 6244.5 39 5999.5 40 5734.0 41 5436.5 42 5040.0 43 4772.5 44 4749.0 45 5705.0 46 10345.5 47 25697.0 48 45923.5 49 45860.5 50 29966.5 51 17173.0 52 7724.0 53 2896.5 54 1670.5 55 1562.5 56 1582.5 57 1204.5 58 818.0 59 640.5 60 585.5 61 569.0 62 529.5 63 474.0 64 447.5 65 440.0 66 410.5 67 383.0 68 326.5 69 262.0 70 249.0 71 202.5 72 147.5 73 135.0 74 116.0 75 89.5 76 63.5 77 45.0 78 34.0 79 22.5 80 22.0 81 23.0 82 21.0 83 14.0 84 6.5 85 2.0 86 1.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01813655656996762 2 0.017844031464000397 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.925251059672196E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.462625529836098E-4 26-27 0.0 28-29 0.0 30-31 2.925251059672196E-4 32-33 8.77575317901659E-4 34-35 0.0027789885066885865 36-37 0.08614864370734618 38-39 0.08102945435291983 40-41 0.08687995647226424 42-43 0.08819631944911673 44-45 0.08117571690590346 46-47 0.07693410286937877 48-49 0.07649531521042793 50-51 0.07708036542236238 52-53 0.06216158501803418 54-55 0.0535320943920012 56-57 0.029106248043738354 58-59 0.014918780404328202 60-61 0.009653328496918249 62-63 0.0013163629768524884 64-65 0.004241614036524685 66-67 0.13602417427475713 68-69 0.38042890031036913 70-71 0.3710680969194181 72-73 0.1652766848714791 74-75 0.028374935278820304 76-77 0.029983823361640013 78-79 0.004095351483541075 80-81 8.77575317901659E-4 82-83 0.0 84-85 1.462625529836098E-4 86-87 8.77575317901659E-4 88-89 0.0 90-91 2.925251059672196E-4 92-93 0.0019014131887869275 94-95 0.07181491351495242 96-97 0.0163814059341643 98-99 0.020037969758754545 100-101 0.011554741685705177 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 341851.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.858926842396247 #Duplication Level Percentage of deduplicated Percentage of total 1 82.70794918494553 21.387388072581327 2 7.784024706161835 4.025730508320876 3 3.11428862317447 2.415964850183267 4 1.6086154820755891 1.6638828027415453 5 0.9728616839557008 1.2578579556590446 6 0.6810031787689905 1.0566006827535972 7 0.4411814613287481 0.7985935392905097 8 0.38575097003359765 0.7980084890785751 9 0.2545277661512008 0.5923633395836198 >10 1.6798832565979254 8.311223310740644 >50 0.18778492969377483 3.4210811142866335 >100 0.12556703130125907 6.363006104998961 >500 0.018099752259640948 3.464667355075749 >1k 0.028280862905688983 21.592155646758382 >5k 0.007918641613592913 13.914834240648705 >10k+ 0.0022624690324551185 8.936641987298561 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17561 5.137033385890344 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12989 3.7996086014082158 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8949 2.6178071733006485 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8212 2.402216170202808 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7363 2.153862355236638 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6543 1.913991768343518 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5887 1.7220952988290221 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5542 1.621174137270331 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5072 1.483687337465738 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4937 1.4441964481601632 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4848 1.4181617137290807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4815 1.4085083852321625 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4760 1.3924195044039653 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 4758 1.391834454192031 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4666 1.3649221444430468 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4598 1.3450304372372759 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4016 1.1747808255643541 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3404 0.9957554607124157 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3372 0.9863946573214646 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3136 0.9173587323132008 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3091 0.9041951025446758 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2828 0.8272609996752972 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 2514 0.7354081164015902 TruSeq Adapter, Index 3 (95% over 21bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2477 0.724584687480803 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2164 0.6330243293130633 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1833 0.5361985192379136 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1773 0.5186470128798805 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1682 0.49202722823686346 No Hit ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 1556 0.4551690648849938 RNA PCR Primer, Index 3 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1540 0.45048866318951825 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1435 0.4197735270629601 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1344 0.3931537424199432 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 1177 0.3443020497234175 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1089 0.3185598403983022 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 981 0.28696712895384247 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 965 0.28228672725836695 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 959 0.2805315766225636 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 902 0.26385764558243213 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 839 0.24542856390649728 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 834 0.24396593837666117 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 832 0.24338088816472672 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 760 0.22231908053508692 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 754 0.22056392989928364 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 685 0.20037969758754545 No Hit CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT 600 0.17551506358033178 TruSeq Adapter, Index 3 (96% over 26bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 568 0.16615426018938076 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 562 0.16439910955357745 TruSeq Adapter, Index 3 (96% over 28bp) CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 548 0.16030375807003636 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 534 0.1562084065864953 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 521 0.15240558020892142 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 468 0.1369017495926588 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 460 0.13456154874492104 No Hit ACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAA 456 0.13339144832105215 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 453 0.13251387300315048 No Hit TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC 452 0.13222134789718326 No Hit CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG 421 0.12315306961219946 TruSeq Adapter, Index 3 (95% over 24bp) GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 406 0.11876519302269116 No Hit ACGCAGAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAA 406 0.11876519302269116 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACAAAAAAAAAAAAAAAA 381 0.11145206537351068 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 361 0.1056015632541663 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 342 0.10004358624078913 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.925251059672196E-4 0.0 6 0.0 0.0 0.0 2.925251059672196E-4 0.0 7 0.0 0.0 0.0 2.925251059672196E-4 0.0 8 0.0 0.0 0.0 2.925251059672196E-4 0.0 9 0.0 0.0 0.0 2.925251059672196E-4 0.0 10-11 0.0 0.0 0.0 7.313127649180491E-4 0.0 12-13 0.0 0.0 0.0 8.775753179016589E-4 0.0 14-15 0.0 0.0 0.0 0.0017551506358033178 0.0 16-17 0.0 0.0 0.0 0.003071513612655806 0.0 18-19 0.0 0.0 0.0 0.004680401695475515 0.0 20-21 0.0 0.0 0.0 0.00936080339095103 0.0 22-23 0.0 0.0 0.0 0.03115392378550889 0.0 24-25 0.0 0.0 0.0 0.08234581732977234 0.0 26-27 0.0 0.0 0.0 0.16220517125882328 0.0 28-29 0.0 0.0 0.0 0.37560223606191 0.0 30-31 0.0 0.0 0.0 0.820240397132084 0.0 32-33 0.0 0.0 0.0 1.3713576967743255 0.0 34-35 0.0 0.0 0.0 2.0813161289567677 0.0 36-37 0.0 0.0 0.0 2.9185229822349505 0.0 38-39 0.0 0.0 0.0 3.9694194254221866 0.0 40-41 0.0 0.0 0.0 5.014757891596046 0.0 42-43 0.0 0.0 0.0 5.917344106057902 0.0 44-45 0.0 0.0 0.0 6.770639840164282 0.0 46-47 0.0 0.0 0.0 7.73407127666732 0.0 48-49 0.0 0.0 0.0 8.482935547943402 0.0 50-51 0.0 0.0 0.0 9.213663262649517 0.0 52-53 0.0 0.0 0.0 9.844786178773795 0.0 54-55 0.0 0.0 0.0 10.478541820851774 0.0 56-57 0.0 0.0 0.0 11.396046815717959 0.0 58-59 0.0 0.0 0.0 11.972321274473382 0.0 60-61 0.0 0.0 0.0 12.568779965540543 0.0 62-63 0.0 0.0 0.0 13.132914632398325 0.0 64-65 0.0 0.0 0.0 13.701290913292633 0.0 66-67 0.0 0.0 0.0 14.348502710245107 0.0 68-69 0.0 0.0 0.0 15.10994556107778 0.0 70-71 0.0 0.0 0.0 15.860857508095634 0.0 72-73 0.0 0.0 0.0 16.467554577871645 0.0 74-75 0.0 0.0 0.0 17.29262163925219 0.0 76-77 0.0 0.0 0.0 17.879719526928397 0.0 78-79 0.0 0.0 0.0 18.97581109898757 0.0 80-81 0.0 0.0 0.0 19.92885789422877 0.0 82-83 0.0 0.0 0.0 20.58440665670131 0.0 84-85 0.0 0.0 0.0 21.32946810159982 0.0 86-87 0.0 0.0 0.0 21.99203746661557 0.0 88-89 0.0 0.0 0.0 22.784780503786735 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATAGT 30 1.3742301E-6 79.07976 6 CGTCACT 40 9.0834874E-8 71.20303 1 CCTCTAG 35 3.4252607E-6 67.81241 1 CTCTAGA 35 3.434201E-6 67.78265 2 TAGTCAA 35 3.434201E-6 67.78265 9 GATAGTC 35 3.434201E-6 67.78265 7 TAGATAG 40 7.583396E-6 59.309822 5 TCCATGC 25 0.0050401296 56.937428 7 ATAGTCC 25 0.0050401296 56.937428 3 TAGTCCA 25 0.0050401296 56.937428 4 GAGATGT 45 1.5196278E-5 52.742985 1 GTCACTA 70 7.967719E-8 47.44786 2 ATAGTCA 50 2.8411783E-5 47.447857 8 CTATACA 65 2.5957615E-6 43.817245 1 GGGACAT 45 9.6676877E-4 42.175873 7 AAGTTCG 35 4.3402615E-6 40.669594 14-15 CTAGATA 60 8.332276E-5 39.53988 4 GTTCGAC 30 8.341033E-5 39.53988 16-17 AGTTCGA 30 8.341033E-5 39.53988 14-15 ATAGTAA 170 0.0 39.327805 68-69 >>END_MODULE