FastQCFastQC Report
Fri 27 May 2016
SRR939895_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939895_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737607
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150710.8673422701450902No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109430.6297741664254345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73600.4235710376396964No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31960.18393111906202037No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25700.14790456069755706No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24950.14358827974334817No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18820.10830987674428107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98750.037.5717121
GTATCAA160950.036.2581371
TATCAAC197700.030.4781362
ATCAACG203250.029.5533713
TCAACGC206000.029.2740084
CAACGCA208600.028.9546265
AACGCAG213450.028.3419956
TACATGG141450.026.598772
GTACATG138550.026.4363881
ACGCAGA236300.025.4808717
TATCCCG1501.0711101E-625.3047835
CGCAGAG238750.025.1377248
ACATGGG146350.024.6715133
GAGTACT121100.023.8758212-13
GCAGAGT238350.023.7467469
ATGGGAG34500.023.6544695
CATGGGA99900.023.4620154
ATCCCGT1852.5702866E-723.0827316
AGTACTT122500.022.94462812-13
GTACTTT128400.022.3728714-15
Produced by FastQC (version 0.11.5)