##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939895_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1737607 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.82974343450504 31.0 30.0 33.0 16.0 34.0 2 29.589583835700477 31.0 30.0 33.0 25.0 34.0 3 29.802309728264216 31.0 30.0 33.0 25.0 34.0 4 33.606601492742605 35.0 33.0 37.0 28.0 37.0 5 33.5917465802106 35.0 33.0 37.0 28.0 37.0 6 33.660987208269766 35.0 33.0 37.0 28.0 37.0 7 33.58384721056027 35.0 33.0 37.0 28.0 37.0 8 33.44729389326815 35.0 33.0 37.0 27.0 37.0 9 34.65016197563661 37.0 34.0 39.0 27.0 39.0 10-11 34.47925739249439 37.0 33.5 39.0 27.0 39.0 12-13 34.3433745375105 37.0 33.0 39.0 27.0 39.0 14-15 34.85240247075432 38.0 33.0 40.0 25.5 41.0 16-17 34.73439822698688 38.0 33.0 40.0 25.0 41.0 18-19 34.71611705063343 38.0 33.0 40.0 25.0 41.0 20-21 34.940768251969516 38.0 34.0 40.0 25.0 41.0 22-23 34.8358457925181 38.0 33.5 40.0 25.0 41.0 24-25 34.5896445513859 38.0 33.0 40.0 24.5 41.0 26-27 34.412709260494466 38.0 33.0 40.0 24.0 41.0 28-29 34.394883307905644 38.0 33.0 40.0 24.0 41.0 30-31 34.07343087360951 38.0 33.0 40.0 23.0 41.0 32-33 33.59080189018576 37.0 32.0 40.0 20.0 41.0 34-35 33.32172522325244 37.0 31.5 40.0 18.5 41.0 36-37 33.02176873136446 37.0 31.0 40.0 17.0 41.0 38-39 32.71000088052132 37.0 31.0 40.0 15.5 41.0 40-41 32.372548568232055 37.0 31.0 40.0 14.5 41.0 42-43 31.843322454386982 36.0 30.0 39.0 9.0 41.0 44-45 31.30645824976534 36.0 30.0 39.0 7.5 41.0 46-47 30.71360929139903 35.5 28.5 39.0 2.0 40.0 48-49 30.176084408039333 35.0 27.0 39.0 2.0 40.0 50-51 29.478612540119833 34.5 26.5 38.5 2.0 40.0 52-53 29.590446804139255 35.0 27.0 38.5 2.0 40.0 54-55 29.642904293088137 35.0 27.0 39.0 2.0 40.0 56-57 29.294191954797604 35.0 26.0 39.0 2.0 40.0 58-59 28.864407774600355 34.5 26.0 38.0 2.0 40.0 60-61 28.421245137709505 34.0 25.0 38.0 2.0 40.0 62-63 27.957581892798544 34.0 23.5 38.0 2.0 40.0 64-65 27.403956705975517 33.5 22.5 37.0 2.0 39.5 66-67 26.787275258444517 33.0 21.0 36.5 2.0 39.0 68-69 26.209997715248615 32.0 19.5 36.0 2.0 39.0 70-71 25.624590888503555 32.0 18.0 35.5 2.0 38.5 72-73 24.962928901644617 31.0 13.0 35.0 2.0 38.0 74-75 24.606064835143965 31.0 9.0 35.0 2.0 37.5 76-77 24.049817076013163 31.0 6.5 35.0 2.0 37.0 78-79 23.375387530091672 30.0 2.0 34.0 2.0 36.0 80-81 22.629410447817026 29.5 2.0 34.0 2.0 36.0 82-83 21.869170934509356 29.0 2.0 34.0 2.0 35.0 84-85 21.074251254742876 28.5 2.0 34.0 2.0 35.0 86-87 20.127540059403536 26.5 2.0 33.5 2.0 35.0 88-89 19.1123896255022 25.5 2.0 33.0 2.0 35.0 90-91 17.72737909089915 22.0 2.0 32.0 2.0 34.0 92-93 16.240194704556323 14.0 2.0 31.0 2.0 34.0 94-95 15.722004170102906 10.0 2.0 31.0 2.0 34.0 96-97 15.409092792558962 2.0 2.0 31.0 2.0 34.0 98-99 14.578070587883222 2.0 2.0 31.0 2.0 34.0 100-101 13.073973574001485 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.744039119586084 1101 2 1.1540432614032312 1101 3 1.149424179442729 1101 4 1.0148544357054092 1101 5 1.0741044826732278 1101 6 1.123707568465484 1101 7 1.0928230539359873 1101 8 1.096781752965036 1101 9 1.1910631379258447 1101 10-11 1.2373733216116847 1101 12-13 1.099148155207402 1101 14-15 1.5620047891756386 1101 16-17 1.2827603620678047 1101 18-19 1.266695473689552 1101 20-21 1.1705329614799638 1101 22-23 1.1978272794668996 1101 24-25 1.4826350479401569 1101 26-27 1.3607009462769355 1101 28-29 1.4357197717162933 1101 30-31 1.5101313031667658 1101 32-33 1.5452791279515168 1101 34-35 1.4417225494721535 1101 36-37 1.2392512029428104 1101 38-39 1.5665455543139188 1101 40-41 1.2186295581668034 1101 42-43 1.3997246293564238 1101 44-45 1.5150264859429399 1101 46-47 1.1682310425950533 1101 48-49 1.1279619505370704 1101 50-51 1.1643814946396382 1101 52-53 0.9339286364695845 1101 54-55 0.9594735593006511 1101 56-57 1.1380977091155096 1101 58-59 0.8568837516476364 1101 60-61 0.8640772519185482 1101 62-63 0.5050573862390095 1101 64-65 0.8530646197098015 1101 66-67 1.034192617106033 1101 68-69 0.7150574493074764 1101 70-71 0.6961887417092605 1101 72-73 0.2916138969287907 1101 74-75 0.4683120110695107 1101 76-77 0.42312656631741774 1101 78-79 -0.004653663121484186 1101 80-81 -0.315196681916877 1101 82-83 -0.3107336373286067 1101 84-85 -0.220912663007514 1101 86-87 -0.672539382516149 1101 88-89 -4.301691081693784 1101 90-91 -7.286409483561432 1101 92-93 -9.542417170593842 1101 94-95 -7.8531405199392434 1101 96-97 -5.154499056935171 1101 98-99 -3.925452493756959 1101 100-101 -3.1326188191225253 1102 1 1.9768267475155845 1102 2 1.2709614772464661 1102 3 1.1803023488959 1102 4 1.185946977179384 1102 5 1.080801802531795 1102 6 1.190593511201378 1102 7 1.1775721760323492 1102 8 1.3688870979347882 1102 9 1.5238841079913144 1102 10-11 1.5087907322163403 1102 12-13 1.4418657299801794 1102 14-15 1.673058082852826 1102 16-17 1.7274285962578872 1102 18-19 1.8529545264839626 1102 20-21 1.787104754264078 1102 22-23 1.5066026176122094 1102 24-25 1.8777451376634389 1102 26-27 1.9190751097620478 1102 28-29 1.6573539639900474 1102 30-31 1.903103624571088 1102 32-33 1.909046165234038 1102 34-35 2.0240007708165564 1102 36-37 1.9841546812152302 1102 38-39 1.9604739340190207 1102 40-41 1.815612701607911 1102 42-43 2.0236117561137483 1102 44-45 1.7032587344337315 1102 46-47 1.7015749121203925 1102 48-49 1.5130523527980948 1102 50-51 1.5168734581447332 1102 52-53 1.7024414674133972 1102 54-55 1.7442567169569472 1102 56-57 1.6993926321541188 1102 58-59 1.4929994101432662 1102 60-61 1.4681919719804064 1102 62-63 1.4953829661292524 1102 64-65 1.5041094073862702 1102 66-67 1.601331081738234 1102 68-69 1.5099397492561089 1102 70-71 1.4589504875890213 1102 72-73 1.3709520006338138 1102 74-75 1.4008291458647761 1102 76-77 1.4170825096253843 1102 78-79 1.5111798064889186 1102 80-81 1.4596332414077686 1102 82-83 1.0431708885918702 1102 84-85 0.9608372270558938 1102 86-87 1.1435244194234748 1102 88-89 0.5568694972690018 1102 90-91 -4.936034196729494 1102 92-93 -9.234690058352193 1102 94-95 -7.059436751815596 1102 96-97 -3.6390489929957024 1102 98-99 -2.336668221999483 1102 100-101 -1.6945201992095846 1103 1 2.108450206036526 1103 2 1.2647435088339805 1103 3 1.1817862894381648 1103 4 0.96883062441578 1103 5 1.0489955628719514 1103 6 1.0762049465756007 1103 7 0.9369536292784275 1103 8 1.08858084975072 1103 9 1.3137402081984675 1103 10-11 1.1350471938506104 1103 12-13 1.0269888179694036 1103 14-15 1.1399300442841565 1103 16-17 1.0494923001459142 1103 18-19 0.9751813876577202 1103 20-21 1.1775392684503885 1103 22-23 1.3334928462754405 1103 24-25 1.1089436907942058 1103 26-27 1.2426118722035113 1103 28-29 1.0809179676768679 1103 30-31 0.9398504685222804 1103 32-33 1.0870713309213826 1103 34-35 1.1718498187694948 1103 36-37 1.1514083102678256 1103 38-39 1.2279546701783062 1103 40-41 1.1215014173119684 1103 42-43 0.8319367677484131 1103 44-45 0.8506980755075375 1103 46-47 0.6266411743639964 1103 48-49 1.0039994449311784 1103 50-51 0.9586366813027674 1103 52-53 0.8043560082533183 1103 54-55 0.5140223080357487 1103 56-57 0.5777225660750567 1103 58-59 0.7903317994036136 1103 60-61 0.9386217996568575 1103 62-63 0.8534491859330942 1103 64-65 0.8086627216954696 1103 66-67 0.7982312384026002 1103 68-69 0.9709145795786931 1103 70-71 0.8699472451190857 1103 72-73 0.946764821720496 1103 74-75 0.7173350553288032 1103 76-77 0.7441419532147826 1103 78-79 0.6027945449094823 1103 80-81 0.3956072465128706 1103 82-83 0.3050247296981503 1103 84-85 0.21285991672805338 1103 86-87 0.10793215448622107 1103 88-89 -0.5555980335968513 1103 90-91 -5.653174671234991 1103 92-93 -8.982438484181827 1103 94-95 -7.799814987983048 1103 96-97 -4.890831009348428 1103 98-99 -3.452588245185373 1103 100-101 -2.5960606898204226 1104 1 2.088777719201776 1104 2 1.4593765244361485 1104 3 1.3157417850105233 1104 4 1.0636170507247016 1104 5 0.9362150964739797 1104 6 1.077775521629782 1104 7 1.0233315798143536 1104 8 0.9849440414779451 1104 9 1.1289170809981286 1104 10-11 1.239177698984328 1104 12-13 1.132523034134465 1104 14-15 1.2216633830110695 1104 16-17 1.1144848410636001 1104 18-19 1.2462563597091432 1104 20-21 1.2495342757072052 1104 22-23 1.1510018314970196 1104 24-25 1.307844533419214 1104 26-27 1.2027224835908399 1104 28-29 1.276471263647366 1104 30-31 1.3106135933200207 1104 32-33 1.22139656284552 1104 34-35 1.125133408543256 1104 36-37 1.0259348393490484 1104 38-39 1.1805105857147709 1104 40-41 1.2834794960681037 1104 42-43 1.463504682784741 1104 44-45 1.307723004900616 1104 46-47 1.149210874186494 1104 48-49 1.0674240156508823 1104 50-51 1.005496205392479 1104 52-53 0.9024472309299121 1104 54-55 1.0454155751260572 1104 56-57 1.0051413129492275 1104 58-59 1.0368679535816803 1104 60-61 1.2220396529121302 1104 62-63 1.265444272867871 1104 64-65 1.1353852364787933 1104 66-67 1.153440920132077 1104 68-69 1.2955924354817299 1104 70-71 1.3359894204988443 1104 72-73 1.1364345136977825 1104 74-75 0.9128857464431164 1104 76-77 0.9619489952749376 1104 78-79 0.7616028201392027 1104 80-81 0.920978545778361 1104 82-83 0.9651889091868355 1104 84-85 0.7952942682230564 1104 86-87 0.8856049208067169 1104 88-89 0.4319118342112951 1104 90-91 -2.908416101564587 1104 92-93 -7.310411636388784 1104 94-95 -5.005219850054379 1104 96-97 -2.5918091481694905 1104 98-99 -1.749406091576084 1104 100-101 -1.051713779363734 1105 1 1.874506435884605 1105 2 1.2816218886842883 1105 3 1.1510559655624029 1105 4 1.05972606803347 1105 5 0.9968710810753549 1105 6 0.9692531950847751 1105 7 0.8609562896206953 1105 8 0.9377462469497502 1105 9 0.9971535484229719 1105 10-11 1.0181750247946724 1105 12-13 0.875756287389045 1105 14-15 0.9687513447495846 1105 16-17 0.9472125328852883 1105 18-19 0.9333114267108229 1105 20-21 0.9620832229286975 1105 22-23 0.8321093060040496 1105 24-25 0.9796717800323975 1105 26-27 0.937243498754377 1105 28-29 0.918397084270957 1105 30-31 1.2418133604304273 1105 32-33 1.1585480549551903 1105 34-35 0.9741243427464354 1105 36-37 1.2284725645246368 1105 38-39 1.0930966336444783 1105 40-41 1.1934308427136173 1105 42-43 1.111117748111372 1105 44-45 1.0218909756020764 1105 46-47 1.143245374171805 1105 48-49 0.858547946709681 1105 50-51 1.0987649981868621 1105 52-53 0.8217100288375789 1105 54-55 0.872808662639649 1105 56-57 1.0406758019145208 1105 58-59 0.9165799271924264 1105 60-61 1.087236433599191 1105 62-63 0.8698001164130815 1105 64-65 1.0222207297345456 1105 66-67 1.0116523904955912 1105 68-69 1.0240042313029427 1105 70-71 0.6926420182830881 1105 72-73 0.8599883207447157 1105 74-75 0.9640107978698396 1105 76-77 0.8521033489144827 1105 78-79 0.8088300013669816 1105 80-81 0.6325866594461154 1105 82-83 0.2350602872761165 1105 84-85 0.13221415640666478 1105 86-87 0.34349010697145843 1105 88-89 0.049617504374978694 1105 90-91 -2.9423765654336407 1105 92-93 -7.098190993701662 1105 94-95 -4.874471076949087 1105 96-97 -2.288711973700652 1105 98-99 -1.3216046207717707 1105 100-101 -0.8287036262590295 1106 1 1.7058915847354221 1106 2 1.1485197028768503 1106 3 0.6863742092348133 1106 4 0.6505213050358662 1106 5 0.6978438877529172 1106 6 0.7369546929093147 1106 7 0.7514326116007553 1106 8 -0.4013727578398516 1106 9 -0.5054180550939478 1106 10-11 -0.6345543856331233 1106 12-13 -0.38639268627086665 1106 14-15 0.6210077947083121 1106 16-17 0.7110874948341888 1106 18-19 0.7755432414601344 1106 20-21 0.6284924594339998 1106 22-23 0.6795240056789211 1106 24-25 0.796487683185326 1106 26-27 0.6784966662404699 1106 28-29 0.6079921761474907 1106 30-31 0.5980465508916097 1106 32-33 0.6537443319627414 1106 34-35 0.7726996842381766 1106 36-37 0.5932325339123494 1106 38-39 0.5474323003552684 1106 40-41 0.44191982924186135 1106 42-43 0.7840703450928679 1106 44-45 0.5504722306868004 1106 46-47 0.7283627048717918 1106 48-49 0.7084079154942913 1106 50-51 0.49215161416740116 1106 52-53 0.5890542669252419 1106 54-55 0.6446104737925644 1106 56-57 0.7688887509373181 1106 58-59 0.5324261978991238 1106 60-61 0.30017260075929286 1106 62-63 0.49911845975635316 1106 64-65 0.5350777950497552 1106 66-67 0.44799857724387593 1106 68-69 0.45940037061233596 1106 70-71 0.5113940438394593 1106 72-73 0.6694769869930184 1106 74-75 0.4803004769518786 1106 76-77 0.2083927232703111 1106 78-79 0.244555467177495 1106 80-81 0.12218375108839652 1106 82-83 -0.12954335121829885 1106 84-85 -0.1779596676755304 1106 86-87 -0.1318429413158988 1106 88-89 -0.4800525120580197 1106 90-91 -3.45368127291394 1106 92-93 -7.756917638104271 1106 94-95 -5.5848429413243394 1106 96-97 -2.9430924605579083 1106 98-99 -1.697258552104893 1106 100-101 -1.283978548022164 1107 1 1.7231354797504537 1107 2 0.8654516449569982 1107 3 0.6500333833406238 1107 4 0.8189581744263066 1107 5 0.5783209808733645 1107 6 0.7269738094882356 1107 7 0.6039988552976752 1107 8 0.6663356845374366 1107 9 0.6559954443995508 1107 10-11 0.6635552150123587 1107 12-13 0.6022236889229902 1107 14-15 0.7846737328942837 1107 16-17 0.7787822480016331 1107 18-19 0.7204330594258579 1107 20-21 0.5399082941456612 1107 22-23 0.5103965559142907 1107 24-25 0.36294477073346343 1107 26-27 0.40718812272407945 1107 28-29 0.5805773592688581 1107 30-31 0.811359549037924 1107 32-33 0.8937694878989433 1107 34-35 0.6593942554196488 1107 36-37 0.3702383655157462 1107 38-39 0.44544414393752163 1107 40-41 0.4920922327611166 1107 42-43 0.6754735213116554 1107 44-45 0.43977580421476503 1107 46-47 0.6941440286022811 1107 48-49 0.7258371785155475 1107 50-51 0.8999183886835702 1107 52-53 0.8193856907480104 1107 54-55 0.6672595130516079 1107 56-57 0.6271327957284107 1107 58-59 0.6504877413655876 1107 60-61 0.24852224572560644 1107 62-63 0.23848363472861323 1107 64-65 0.43031217847969216 1107 66-67 0.5688559405088007 1107 68-69 0.4839441575631369 1107 70-71 0.5295188797702508 1107 72-73 0.5855753367185095 1107 74-75 0.46064663680641615 1107 76-77 0.48419503175423984 1107 78-79 0.5806336212922254 1107 80-81 0.5524562307840775 1107 82-83 0.3881271639952466 1107 84-85 0.43027559991631037 1107 86-87 0.8795403613911823 1107 88-89 0.7501853419892406 1107 90-91 -2.5715729091288235 1107 92-93 -3.759915575059823 1107 94-95 -2.013691098998269 1107 96-97 -0.8833195726016747 1107 98-99 -0.21468459413559948 1107 100-101 0.09655233564049048 1108 1 1.7008891072223875 1108 2 0.910097779821804 1108 3 -0.49853495318327745 1108 4 -0.22927018844062275 1108 5 0.40469493370751763 1108 6 0.5769949536367633 1108 7 0.5006511075663198 1108 8 0.5161940414779451 1108 9 0.6801028051399456 1108 10-11 0.5950674232951059 1108 12-13 0.45994842697048455 1108 14-15 0.3417802130074108 1108 16-17 0.37864022601474545 1108 18-19 0.5353397599231613 1108 20-21 0.37322086225785256 1108 22-23 0.3687433411700738 1108 24-25 0.2525821675325872 1108 26-27 0.408427670011946 1108 28-29 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1.4187701522740213 2201 38-39 1.5435340261534165 2201 40-41 1.6577213257316004 2201 42-43 1.3389169972432349 2201 44-45 1.49973450268714 2201 46-47 1.485394240794335 2201 48-49 1.4000662400271047 2201 50-51 1.1560238352835874 2201 52-53 1.2033201440400632 2201 54-55 1.1807597552230824 2201 56-57 1.0858251943473363 2201 58-59 1.1840404047067459 2201 60-61 1.0815751481234948 2201 62-63 1.2480438793607362 2201 64-65 1.2859776672533663 2201 66-67 1.4021670553229946 2201 68-69 1.3217961043570519 2201 70-71 1.2612034743938878 2201 72-73 0.8050657020314524 2201 74-75 0.8154350131985169 2201 76-77 1.102791153584402 2201 78-79 1.029206055481545 2201 80-81 0.9508309564917852 2201 82-83 1.1405966122336224 2201 84-85 1.0962857820199154 2201 86-87 1.5498037287115842 2201 88-89 1.9309964840574594 2201 90-91 2.506028798997704 2201 92-93 3.148297542068631 2201 94-95 2.779549591416254 2201 96-97 1.8545938904192862 2201 98-99 1.298030264914372 2201 100-101 0.6189187013708128 2202 1 -16.354097813319775 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2.6853186480984874 2202 64-65 2.6856141788155163 2202 66-67 2.5897537534821815 2202 68-69 2.61132739908577 2202 70-71 2.6455385382741596 2202 72-73 2.361575820597924 2202 74-75 2.1748952605486487 2202 76-77 2.2145214517507377 2202 78-79 2.0881066693894788 2202 80-81 2.226828315997409 2202 82-83 2.6496358205618336 2202 84-85 2.6768632570191393 2202 86-87 2.7451072857055827 2202 88-89 3.393405703923815 2202 90-91 4.211812694014224 2202 92-93 4.750131242078899 2202 94-95 4.1249753798942965 2202 96-97 3.5907042277655687 2202 98-99 3.165586486666845 2202 100-101 2.3774797230347104 2203 1 2.35424463974287 2203 2 1.5672376323896167 2203 3 1.4160209995154673 2203 4 1.1772554596519882 2203 5 1.167418442311579 2203 6 1.1599827608401156 2203 7 1.066541149364575 2203 8 1.0998873460135812 2203 9 1.183496530431647 2203 10-11 1.217650473793924 2203 12-13 1.005655091464618 2203 14-15 1.195786900498092 2203 16-17 0.9531011070254891 2203 18-19 1.1536875152167028 2203 20-21 1.4007973720279239 2203 22-23 1.284597328067818 2203 24-25 1.5343925280007014 2203 26-27 1.4987863642698613 2203 28-29 1.5745143938811594 2203 30-31 1.7428685523969776 2203 32-33 1.8857843986607534 2203 34-35 1.7806622820228526 2203 36-37 1.7148238071789947 2203 38-39 1.6637168634230832 2203 40-41 1.694939013517235 2203 42-43 1.7801893480115218 2203 44-45 1.85318417063894 2203 46-47 1.678747458628166 2203 48-49 1.811848014362564 2203 50-51 2.0904127015464695 2203 52-53 1.9326244220177848 2203 54-55 1.7453778018321309 2203 56-57 1.9320428845077124 2203 58-59 1.9351630636570825 2203 60-61 1.7163310306545405 2203 62-63 1.7997031111081796 2203 64-65 1.7968891582703144 2203 66-67 1.7524560910236708 2203 68-69 1.7807862515553907 2203 70-71 1.837177088643287 2203 72-73 1.6765831439126302 2203 74-75 1.4769635807426909 2203 76-77 1.3285394048842747 2203 78-79 1.2098676168502003 2203 80-81 1.301276279854374 2203 82-83 1.3454410548046596 2203 84-85 1.376870965771463 2203 86-87 1.654020289682471 2203 88-89 2.0015792871308484 2203 90-91 2.7072418168545695 2203 92-93 3.2391590032232056 2203 94-95 2.8594262414162 2203 96-97 2.0575753484392028 2203 98-99 1.4844461212995181 2203 100-101 1.126455388155497 2204 1 2.391955468198386 2204 2 1.66164742952472 2204 3 1.3991252284080922 2204 4 1.2694321309482461 2204 5 1.2033230875170347 2204 6 1.2257357158552082 2204 7 1.0422337483644455 2204 8 0.8147337926948168 2204 9 1.1573306734568334 2204 10-11 1.2723962239338036 2204 12-13 1.1244880780652835 2204 14-15 1.2900482022115867 2204 16-17 1.1181824597384704 2204 18-19 1.0686519113586854 2204 20-21 1.1456553712733282 2204 22-23 1.1453708242194907 2204 24-25 1.4524916814745836 2204 26-27 1.6404254441764579 2204 28-29 1.6878692573471383 2204 30-31 1.6410220110991887 2204 32-33 1.8804430898919051 2204 34-35 1.799870464135175 2204 36-37 1.861880122717011 2204 38-39 1.8494136734435642 2204 40-41 1.9696723350137617 2204 42-43 1.9057031684526713 2204 44-45 1.9673527656532315 2204 46-47 2.253273762613624 2204 48-49 2.1810632635136393 2204 50-51 1.9016299026019716 2204 52-53 1.8462335236628284 2204 54-55 1.898062780111207 2204 56-57 1.951155427975845 2204 58-59 1.7769796664089093 2204 60-61 1.8510416383381063 2204 62-63 1.997329929410526 2204 64-65 1.7743045669846147 2204 66-67 1.7934278322135242 2204 68-69 1.6621027824687218 2204 70-71 1.832570372269835 2204 72-73 1.5162610913642318 2204 74-75 1.4779557404180501 2204 76-77 1.6658649337148717 2204 78-79 1.5656312541908015 2204 80-81 1.8176753140646795 2204 82-83 1.853326789548909 2204 84-85 1.9218395262098262 2204 86-87 2.558731931139473 2204 88-89 2.6622120557630105 2204 90-91 3.269855196102238 2204 92-93 3.8226548979161503 2204 94-95 3.373915861045978 2204 96-97 2.571232692844619 2204 98-99 2.339326378796711 2204 100-101 1.9096694481750962 2205 1 2.0931572582198044 2205 2 1.2765090100187777 2205 3 0.9997551532072535 2205 4 1.052434534297781 2205 5 0.9074837549341979 2205 6 1.0569050933614577 2205 7 0.9663119913519864 2205 8 0.9994119423836452 2205 9 1.1590811048697418 2205 10-11 0.9666964158617617 2205 12-13 0.9405592282759159 2205 14-15 1.107928851891053 2205 16-17 0.9804700416449705 2205 18-19 0.9189638051007236 2205 20-21 1.1315625975440327 2205 22-23 1.2394446270180524 2205 24-25 1.4125432349212375 2205 26-27 1.2491386163241174 2205 28-29 1.353536137139038 2205 30-31 1.3414933741853474 2205 32-33 1.5794270905165888 2205 34-35 1.6478442713422297 2205 36-37 1.5581592385863559 2205 38-39 1.3231317021379567 2205 40-41 1.3981803940576185 2205 42-43 1.7893571068657188 2205 44-45 1.7194702113891367 2205 46-47 1.7678118518570969 2205 48-49 1.6411012745279585 2205 50-51 1.5194634707188222 2205 52-53 1.3251691345835575 2205 54-55 1.4683642836381452 2205 56-57 1.4837251217987344 2205 58-59 1.3794940062599927 2205 60-61 1.6162603788746104 2205 62-63 1.6262348681627117 2205 64-65 1.6422936569706188 2205 66-67 1.7046992562694356 2205 68-69 1.6092613640631726 2205 70-71 1.5943153348451773 2205 72-73 1.360544585761268 2205 74-75 1.3846420426511372 2205 76-77 1.3918722817810298 2205 78-79 1.2428681254509648 2205 80-81 1.070847340701203 2205 82-83 1.2160647293646143 2205 84-85 1.5782256618709987 2205 86-87 1.8430993828542164 2205 88-89 2.110840567539018 2205 90-91 2.8584044068521663 2205 92-93 3.440970807133448 2205 94-95 3.092194370099092 2205 96-97 2.2095357914972045 2205 98-99 1.7751866819800757 2205 100-101 1.3926479073517228 2206 1 -6.091033589811694 2206 2 -1.5476247708409296 2206 3 -0.3196149578055305 2206 4 0.13185908085257125 2206 5 0.5859204324624017 2206 6 0.6613949337307119 2206 7 0.6719297650435863 2206 8 0.6817221549829355 2206 9 1.034506190068761 2206 10-11 1.1602026734014572 2206 12-13 0.7514368740087178 2206 14-15 1.031879650492705 2206 16-17 1.065553992034829 2206 18-19 1.082969772741201 2206 20-21 1.0804497680968836 2206 22-23 0.9760354234248112 2206 24-25 1.0592949498899742 2206 26-27 1.1663422241205694 2206 28-29 1.1572346128374775 2206 30-31 1.1053710918680224 2206 32-33 1.2843095476525193 2206 34-35 1.3459430028126889 2206 36-37 1.223535753418247 2206 38-39 1.3733816929692466 2206 40-41 1.2607863626499594 2206 42-43 1.461862538801558 2206 44-45 1.1008660166272506 2206 46-47 1.1169580139750117 2206 48-49 0.9933808958308354 2206 50-51 1.2672438729718287 2206 52-53 1.149663364735261 2206 54-55 1.2688423723806217 2206 56-57 1.243517094264046 2206 58-59 1.113134017783171 2206 60-61 1.2807774146180257 2206 62-63 1.189349049259718 2206 64-65 1.1938421051349302 2206 66-67 1.3568361536507147 2206 68-69 1.4600177196590671 2206 70-71 1.394876991831275 2206 72-73 1.2096378353229191 2206 74-75 1.4928928739241947 2206 76-77 1.5368140513728825 2206 78-79 1.565506916625818 2206 80-81 1.4479022166212587 2206 82-83 1.1571214015119757 2206 84-85 1.4399309971644314 2206 86-87 1.7500260564097836 2206 88-89 2.33438547924084 2206 90-91 3.0910429992549844 2206 92-93 3.7777683264584265 2206 94-95 2.8150477790815156 2206 96-97 1.6401863275797925 2206 98-99 1.3649237177738804 2206 100-101 1.2159101708164979 2207 1 1.7998869365456542 2207 2 1.1337368098647467 2207 3 1.021330481492658 2207 4 0.7916071198093348 2207 5 0.6818848083183582 2207 6 0.7905487922415446 2207 7 0.6569836341730451 2207 8 0.6762976684209505 2207 9 0.8865521823525526 2207 10-11 0.7286104778226132 2207 12-13 0.5511074241899152 2207 14-15 0.6462958027910943 2207 16-17 0.6255772985674639 2207 18-19 0.6459163338024112 2207 20-21 0.7115209700986327 2207 22-23 0.887537714598551 2207 24-25 0.8902481217293712 2207 26-27 0.6396375935808436 2207 28-29 0.6843995201694995 2207 30-31 0.851247115272578 2207 32-33 1.0343740762533926 2207 34-35 0.9791523613657276 2207 36-37 1.1046759765183936 2207 38-39 1.0286451303016264 2207 40-41 1.1045091183751694 2207 42-43 1.1760694496241193 2207 44-45 1.1666344106819082 2207 46-47 1.2863756773862036 2207 48-49 0.9330118031247459 2207 50-51 1.1893993732863066 2207 52-53 1.037368488088351 2207 54-55 1.0717106278485389 2207 56-57 0.9321380064316394 2207 58-59 0.9424214259932775 2207 60-61 0.9613684639293112 2207 62-63 1.042598622472454 2207 64-65 0.8817242056634136 2207 66-67 1.0588418495930334 2207 68-69 0.9945629836137186 2207 70-71 1.0773135046730076 2207 72-73 1.1290817730378429 2207 74-75 1.0644262313990325 2207 76-77 1.0782350147277135 2207 78-79 1.1904473569994884 2207 80-81 1.3930549479236198 2207 82-83 1.496944260973038 2207 84-85 1.738622999702013 2207 86-87 2.0708039936182807 2207 88-89 2.2384913497098857 2207 90-91 3.014456535013906 2207 92-93 3.839168515415599 2207 94-95 3.3658645968140863 2207 96-97 2.4689415790371303 2207 98-99 2.1512785052787446 2207 100-101 1.6804798848486104 2208 1 1.7418558484627518 2208 2 1.0327005404346927 2208 3 0.8280662327879966 2208 4 0.7346123011564814 2208 5 0.757532476712143 2208 6 0.6527249580446508 2208 7 0.3217504735512833 2208 8 0.5897691710116248 2208 9 0.8067594362385648 2208 10-11 0.7260198042474855 2208 12-13 0.7130245226355072 2208 14-15 0.7538087561589677 2208 16-17 0.8102923244742044 2208 18-19 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2.7644348330363115 2311 92-93 3.757786099442944 2311 94-95 3.2362332591584444 2311 96-97 2.5900114865324486 2311 98-99 2.5963887784523916 2311 100-101 2.470619048779925 2312 1 1.2488838669240216 2312 2 0.39298812697190044 2312 3 -0.4580170606329972 2312 4 -0.6033122209839803 2312 5 -0.315452675898122 2312 6 -0.4643196058949144 2312 7 -0.029903530019055324 2312 8 0.2383579070241666 2312 9 0.06616369016811774 2312 10-11 -0.24404322096259534 2312 12-13 -0.17091248480995347 2312 14-15 0.02587519931653759 2312 16-17 -0.30543213888627463 2312 18-19 -0.6569736823076582 2312 20-21 0.03234378108830782 2312 22-23 0.24290438199090403 2312 24-25 0.26112617502322166 2312 26-27 0.12583218799892393 2312 28-29 0.1768702390269894 2312 30-31 0.05849202100689155 2312 32-33 0.3342361065577464 2312 34-35 0.19140662835749112 2312 36-37 0.0839290896807654 2312 38-39 0.19131194573246546 2312 40-41 -0.03187460609907333 2312 42-43 -0.09243019116484064 2312 44-45 -0.16092333521437752 2312 46-47 -0.07651428429795004 2312 48-49 0.1558067371605354 2312 50-51 -0.09216983470777151 2312 52-53 -0.0684588946782263 2312 54-55 -0.11611647190328256 2312 56-57 -0.01992364724537765 2312 58-59 0.048523931762424866 2312 60-61 0.1858416210678122 2312 62-63 -0.07572114066596214 2312 64-65 0.052484971109489464 2312 66-67 0.17580150910450598 2312 68-69 0.3204708078833214 2312 70-71 0.34758089919558444 2312 72-73 0.39991029874864736 2312 74-75 0.06740577814000659 2312 76-77 0.14521045775466845 2312 78-79 0.2157390799062675 2312 80-81 0.32180598201601285 2312 82-83 0.6027593979086383 2312 84-85 0.6755164228743915 2312 86-87 1.210792890731529 2312 88-89 1.4992124386636014 2312 90-91 2.1448904184118227 2312 92-93 3.0699602492075595 2312 94-95 2.7345754101549655 2312 96-97 1.888172976259229 2312 98-99 1.6688776692966858 2312 100-101 1.5086393092401291 2313 1 -7.387415933807503 2313 2 -2.5884260455613877 2313 3 -1.74049487235051 2313 4 -0.7726251489632858 2313 5 -0.8806879743856939 2313 6 -0.37459553373874854 2313 7 -0.27181016006576897 2313 8 -0.23315593252101507 2313 9 -0.028147162097909018 2313 10-11 -0.35861358108792274 2313 12-13 -0.5262872985420017 2313 14-15 -0.46611800982358886 2313 16-17 -0.6940153607865795 2313 18-19 -0.8125303667599155 2313 20-21 -0.5757519923224663 2313 22-23 -1.179518428787084 2313 24-25 -0.5536883818447649 2313 26-27 -0.610112365329428 2313 28-29 -0.7298040531547727 2313 30-31 -0.180200940577123 2313 32-33 -0.5195368573860293 2313 34-35 -0.27040912368435244 2313 36-37 -0.09431227316703428 2313 38-39 -0.34097918834987695 2313 40-41 -0.35493370493362164 2313 42-43 -0.4146154420202528 2313 44-45 -0.5681087717915041 2313 46-47 -1.329180893063139 2313 48-49 -0.6565535618732738 2313 50-51 -0.3942090591338925 2313 52-53 -0.6088367072697203 2313 54-55 -0.48556885504062564 2313 56-57 -0.21920960187683747 2313 58-59 -0.22844272463492388 2313 60-61 -0.281185673469345 2313 62-63 -0.2552026056388499 2313 64-65 -0.37577810479169926 2313 66-67 -0.23536315176763978 2313 68-69 -0.2648634038044726 2313 70-71 -0.3287502611936901 2313 72-73 -0.14029695029761768 2313 74-75 0.13067310925341857 2313 76-77 0.40823118866793706 2313 78-79 0.14030711304776133 2313 80-81 0.23664656723606114 2313 82-83 0.4313777212129999 2313 84-85 0.40695804398461277 2313 86-87 0.9322938936287883 2313 88-89 1.2967414931871275 2313 90-91 2.186471871585919 2313 92-93 3.1395303744495475 2313 94-95 2.70954189831029 2313 96-97 1.8429068588918458 2313 98-99 1.4130602097075453 2313 100-101 1.0290280029391372 2314 1 0.576525520402388 2314 2 0.06421330139179915 2314 3 -1.3465025609339136 2314 4 -0.29849059737432526 2314 5 -0.1457192652924988 2314 6 -3.7176561527152323 2314 7 -2.9327223896584016 2314 8 -2.458872271519404 2314 9 -3.5803298011276077 2314 10-11 -2.672786561800258 2314 12-13 -1.1404691071936526 2314 14-15 -1.4125317284413015 2314 16-17 -2.228909308268612 2314 18-19 -2.943904449309425 2314 20-21 -2.9974343768592178 2314 22-23 -4.205467466748601 2314 24-25 -3.168110751843013 2314 26-27 -1.7225914851838837 2314 28-29 -1.8583898862432733 2314 30-31 -1.9537060876795564 2314 32-33 -2.1136124824799936 2314 34-35 -1.8448948617345593 2314 36-37 -2.213546697716424 2314 38-39 -3.4250828283575423 2314 40-41 -2.541825456465748 2314 42-43 -1.4144130890985487 2314 44-45 -2.2818689084038652 2314 46-47 -1.8304252186982382 2314 48-49 -2.231106337087734 2314 50-51 -2.8880205357308846 2314 52-53 -1.9270018476684392 2314 54-55 -2.5588448895302776 2314 56-57 -1.8450022197884515 2314 58-59 -1.0636209593505193 2314 60-61 -1.3016419399958714 2314 62-63 -1.1154331527917698 2314 64-65 -1.3954991694963574 2314 66-67 -0.8546124734646448 2314 68-69 -0.7765595957900864 2314 70-71 -0.7831433334683027 2314 72-73 -1.8789658073967281 2314 74-75 -1.7605321941311303 2314 76-77 -0.8374361552671985 2314 78-79 -1.7696425263171243 2314 80-81 -0.9340234319776997 2314 82-83 -0.6600697987567621 2314 84-85 -1.7465325094306259 2314 86-87 -0.46159248251591123 2314 88-89 0.5153140981602924 2314 90-91 1.6088367661816996 2314 92-93 2.5565473309898774 2314 94-95 2.1142249821853714 2314 96-97 1.3722826546133309 2314 98-99 1.3104887965061902 2314 100-101 1.3798820371005025 2315 1 -3.0747017247454487 2315 2 -2.0732180757505496 2315 3 -2.934859991990951 2315 4 -4.424754909414261 2315 5 -4.100776295197736 2315 6 -3.0490610861403304 2315 7 -6.139234522426989 2315 8 -3.292911827968023 2315 9 -2.9682751676569694 2315 10-11 -3.9521206236186686 2315 12-13 -3.0866024374229255 2315 14-15 -2.331752821721828 2315 16-17 -2.4928072466364455 2315 18-19 -1.9207674334888836 2315 20-21 -1.476596890627448 2315 22-23 -0.8500642851797551 2315 24-25 -2.378407854441548 2315 26-27 -4.304591016664187 2315 28-29 -3.543203445959893 2315 30-31 -3.385237754227397 2315 32-33 -4.199954551329114 2315 34-35 -4.040244294491075 2315 36-37 -3.3749902912915957 2315 38-39 -1.9785854495942914 2315 40-41 -1.7517650581658089 2315 42-43 -3.203870522920141 2315 44-45 -6.616493241454439 2315 46-47 -3.5162287809029564 2315 48-49 -2.442551167438495 2315 50-51 -3.5693463882694054 2315 52-53 -3.2113445596408674 2315 54-55 -1.8798514502610537 2315 56-57 -1.4924709766543778 2315 58-59 -2.824381998300222 2315 60-61 -3.059804004159787 2315 62-63 -4.615373205551158 2315 64-65 -4.018160826979358 2315 66-67 -3.454630079329 2315 68-69 -2.021997858134924 2315 70-71 -3.3077336333645597 2315 72-73 -2.6843839030242194 2315 74-75 -1.484784345029393 2315 76-77 -3.131557106100278 2315 78-79 -1.6746812955944534 2315 80-81 -1.2964870016612728 2315 82-83 -0.9891098717410713 2315 84-85 -2.187810113170638 2315 86-87 -1.5889778952570275 2315 88-89 -1.555325140218745 2315 90-91 -0.5806733397299375 2315 92-93 0.0313757465639366 2315 94-95 -0.2523953175217315 2315 96-97 -0.5456756180331208 2315 98-99 -0.8518979475874797 2315 100-101 -1.0199660577558198 2316 1 -7.440442851852282 2316 2 -6.233011677389477 2316 3 -3.597911757006404 2316 4 -4.230401402623066 2316 5 -2.303085357557684 2316 6 -2.8062893797275628 2316 7 -3.2494166059586895 2316 8 -2.354948351591897 2316 9 -7.378839392277641 2316 10-11 -7.166643974853759 2316 12-13 -6.141320010299843 2316 14-15 -4.873253753834831 2316 16-17 -4.777691691055814 2316 18-19 -4.092866203907533 2316 20-21 -2.8823262152264064 2316 22-23 -3.2917695782808103 2316 24-25 -3.265313901548822 2316 26-27 -4.25956893373835 2316 28-29 -4.704232093622366 2316 30-31 -7.472358170991949 2316 32-33 -5.223741029884273 2316 34-35 -5.784383802866429 2316 36-37 -4.582408526849335 2316 38-39 -6.793914068062335 2316 40-41 -4.439980755976464 2316 42-43 -5.242302030804861 2316 44-45 -3.6714524182606993 2316 46-47 -4.799235634613929 2316 48-49 -5.788423601157792 2316 50-51 -5.248117703839181 2316 52-53 -6.654306430769001 2316 54-55 -5.550133365070945 2316 56-57 -4.87093573589242 2316 58-59 -3.8010588290625336 2316 60-61 -3.9431348477005237 2316 62-63 -4.88139056598888 2316 64-65 -3.713541187594384 2316 66-67 -4.51504368387252 2316 68-69 -3.6322671091568033 2316 70-71 -3.1111335218733274 2316 72-73 -2.6832214718263963 2316 74-75 -2.989437336745361 2316 76-77 -3.6427519659803913 2316 78-79 -5.452822361995885 2316 80-81 -6.276036647185098 2316 82-83 -4.223065781919651 2316 84-85 -2.879538645985857 2316 86-87 -2.64158354982845 2316 88-89 -1.7238222746803196 2316 90-91 -1.504207255308451 2316 92-93 -1.5179390753842146 2316 94-95 -1.9936767122966135 2316 96-97 -3.313158788173668 2316 98-99 -4.466850038137126 2316 100-101 -3.967063113039016 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15042.0 3 5113.0 4 4929.0 5 5477.0 6 6343.0 7 7833.0 8 9844.0 9 11821.0 10 14525.0 11 17784.0 12 20849.0 13 24097.0 14 27446.0 15 31335.0 16 35616.0 17 37596.0 18 31669.0 19 28078.0 20 29381.0 21 31460.0 22 34259.0 23 37356.0 24 41689.0 25 46908.0 26 52573.0 27 58538.0 28 66067.0 29 73907.0 30 83172.0 31 93111.0 32 102418.0 33 111676.0 34 126418.0 35 139737.0 36 141466.0 37 103982.0 38 27684.0 39 408.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.171866220620146 19.347123893167822 14.079856158640421 20.40115372757161 2 15.996798646627287 20.790934799794414 40.35347412527456 22.858792428303744 3 19.788057946962624 27.040648157752194 29.664453894824188 23.506840000460993 4 12.539324236869206 16.943754023188546 37.26891698213459 33.24800475780766 5 12.883336953909962 38.94664845046043 32.588128388528396 15.581886207101208 6 29.910395773133253 36.732603882235104 17.648597902472705 15.708402442158938 7 27.58186928010377 35.32636737046912 19.74173092166895 17.350032427758162 8 27.57927209909164 32.79773402064352 19.32503650110469 20.297957379160163 9 28.836312675574042 16.127072227695574 19.568943203508223 35.46767189322216 10-11 25.345835562237145 27.235292693499595 25.684321506308567 21.734550237954696 12-13 27.592394009249126 25.08999411806917 26.597307337946464 20.720304534735238 14-15 24.025580835700193 25.412266177875576 26.966397053419737 23.595755933004494 16-17 21.3241386306333 29.006656831804552 27.18130911255979 22.487895425002353 18-19 21.390189060800775 26.22648269318181 31.57802198815765 20.80530625785976 20-21 23.137806539018644 24.818753738280716 31.64792782145611 20.395511901244532 22-23 22.255591269888182 25.337353382443982 31.638999512968898 20.768055834698934 24-25 21.625069364088496 25.58508103917499 31.96804810320223 20.821801493534288 26-27 21.077360990842813 25.867446101374586 31.961361330061905 21.09383157772069 28-29 20.982472758445038 26.487490719497792 31.7132138775741 20.816822644483068 30-31 21.240227488578128 25.970459017821323 31.501786285446308 21.28752720815424 32-33 20.885052077580063 26.02532107032961 31.48601867022993 21.603608181860395 34-35 21.609218672090634 26.14663117207982 31.358809956594975 20.885340199234566 36-37 21.675773189186383 25.707100203472738 31.296212176927547 21.320914430413335 38-39 21.1599519909288 26.243440134868344 31.14052112104988 21.456086753152974 40-41 21.488204156555017 26.22678646358292 31.132865277495398 21.152144102366666 42-43 21.152561805969686 26.22444047906043 30.717131497081134 21.905866217888754 44-45 21.311859712133753 26.247430486533 30.79702278126531 21.64368702006794 46-47 21.906425503370414 26.685769623645218 29.761622551640563 21.646182321343804 48-49 21.465606078265516 27.018895337096094 29.404613397987223 22.11088518665117 50-51 21.501719482078148 27.348564405566968 29.289588478316062 21.86012763403882 52-53 21.853196837826523 27.322114759050308 28.708717615231194 22.11597078789197 54-55 21.289221797191296 27.338464285910057 29.00668262480046 22.365631292098183 56-57 21.68092177180564 27.352294247982872 28.56920355044127 22.39758042977022 58-59 21.66850252823953 26.906000714731338 28.695309568372068 22.73018718865706 60-61 21.264552591172208 27.157330180722454 29.202067459144615 22.37604976896072 62-63 21.449886689309583 27.09731456546936 29.026767848596158 22.426030896624898 64-65 21.136204334902704 27.35072011468552 28.851498457298703 22.661577093113074 66-67 21.33877314691332 27.228950542544418 28.576495287933056 22.855781022609214 68-69 21.590147262236123 27.033870227803614 28.714997068942 22.660985441018262 70-71 21.611774215269072 27.07287455580384 28.311505253647795 23.00384597527929 72-73 21.567501347802974 27.109776785366595 28.364149392818007 22.958572474012424 74-75 21.407412813380436 27.645901416141644 28.048564142046278 22.89812162843164 76-77 21.520448501235677 27.108336059723232 28.23732113295493 23.133894306086166 78-79 21.65398627448039 27.348103595707627 28.06934537787876 22.928564751933216 80-81 21.841017270645384 27.272569891768857 27.814692210190767 23.071720627394992 82-83 21.75682370392256 27.215873401497998 27.830128659195807 23.19717423538363 84-85 21.796098510799407 27.269116215339128 27.725698626185412 23.209086647676052 86-87 21.6098496048648 27.192340478316247 27.759362014230348 23.438447902588607 88-89 21.7740576842081 27.200602343601254 27.35903486554006 23.66630510665059 90-91 21.7713142247426 26.97723858533464 27.60868446895716 23.6427627209656 92-93 22.198675634694215 26.535322244955196 27.823480089803766 23.442522030546826 94-95 21.737872554703724 26.537715713879834 27.93891754422842 23.78549418718802 96-97 22.05557620474557 26.3269284932658 27.555741270009836 24.061754031978797 98-99 22.189731379956953 26.425773852961008 27.077463812963597 24.307030954118442 100-101 22.534396749696988 26.30797729730791 27.215195720927117 23.942430232067984 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3754.0 1 2425.0 2 1159.0 3 1299.5 4 1243.5 5 1197.5 6 1625.5 7 2963.5 8 4689.0 9 5292.5 10 4631.0 11 3833.5 12 3612.0 13 3615.0 14 3581.0 15 3662.5 16 3944.5 17 4352.0 18 4735.5 19 5100.0 20 5629.5 21 6315.0 22 7114.0 23 8124.5 24 9287.0 25 10821.0 26 12915.0 27 15217.5 28 17312.0 29 19543.0 30 21671.0 31 24426.0 32 28232.5 33 30489.5 34 32044.5 35 34419.0 36 37432.5 37 40324.0 38 41968.0 39 43551.0 40 45310.5 41 47229.5 42 49276.5 43 51525.5 44 53909.0 45 56091.5 46 58288.5 47 61550.5 48 65479.0 49 65402.5 50 61917.5 51 58899.5 52 56970.0 53 56392.5 54 55735.0 55 53216.5 56 49765.0 57 46066.5 58 42979.5 59 39077.5 60 33200.5 61 27421.0 62 22247.0 63 16832.0 64 12127.0 65 9222.5 66 7089.0 67 5359.0 68 4038.0 69 3082.0 70 2312.0 71 1784.5 72 1390.0 73 1143.0 74 934.0 75 710.5 76 537.0 77 389.0 78 281.5 79 220.5 80 160.0 81 106.5 82 73.5 83 47.5 84 32.0 85 25.0 86 19.0 87 11.0 88 8.0 89 8.5 90 6.5 91 7.0 92 5.0 93 3.5 94 3.5 95 1.5 96 2.5 97 5.0 98 2.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13593407485121778 2 0.1203379130033431 3 0.12816476913364183 4 0.13547367154943551 5 0.12407868983032412 6 0.1393295492018621 7 0.1716153307393444 8 0.15699752590775706 9 0.16919821340498745 10-11 0.1624648151164216 12-13 0.1515014614927311 14-15 0.15622059533599947 16-17 0.15092595736550327 18-19 0.16692497210243742 20-21 0.17377347121644882 22-23 0.20899432380279315 24-25 0.23025920130386213 26-27 0.2425174392138153 28-29 0.23791340619599252 30-31 0.22913696825576785 32-33 0.22971247238299572 34-35 0.21992890222012226 36-37 0.22787085917586658 38-39 0.2168499551394533 40-41 0.2209648096491324 42-43 0.21368468243970012 44-45 0.20559884945214885 46-47 0.2212237865063849 48-49 0.25788339941079885 50-51 0.260530718396047 52-53 0.26234355639681467 54-55 0.2607321448405767 56-57 0.23457548225807098 58-59 0.23529486241710582 60-61 0.20686495853205009 62-63 0.1978007685282115 64-65 0.19990135859259314 66-67 0.2072678114211096 68-69 0.20669230729388177 70-71 0.21388610888422988 72-73 0.2434670210237413 74-75 0.24576903753265267 76-77 0.2616241762377799 78-79 0.25811360106169 80-81 0.2702279629398362 82-83 0.2702279629398362 84-85 0.2471502474379995 86-87 0.24974001601052484 88-89 0.25189815648762925 90-91 0.43335460780256985 92-93 0.5791010280230224 94-95 0.34041069125527235 96-97 0.28539249669229 98-99 0.3162395179117027 100-101 0.3355189061738356 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1737607.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.942366904535945 #Duplication Level Percentage of deduplicated Percentage of total 1 75.52516979965816 39.98481250060879 2 12.292338028567523 13.015709400460034 3 4.921441454056962 7.816582774796299 4 2.43849820640619 5.163994669584373 5 1.3849702211116688 3.6661800798975124 6 0.8678250237995063 2.756682649135866 7 0.5576625733266777 2.0666783596192055 8 0.38451443778700645 1.6285683556328843 9 0.28105106412813013 1.3391557700383552 >10 1.2282267493562737 11.071327353928769 >50 0.07095980047701675 2.5945883847787505 >100 0.04141125816677621 4.095457263368079 >500 0.003916951142118523 1.4563485982097037 >1k 0.0016786933466222242 1.366526609809138 >5k 1.1191288977481494E-4 0.43607508880485846 >10k+ 2.2382577954962988E-4 1.5413121413273896 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15071 0.8673422701450902 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10943 0.6297741664254345 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7360 0.4235710376396964 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3196 0.18393111906202037 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2570 0.14790456069755706 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2495 0.14358827974334817 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1882 0.10830987674428107 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.755041272278484E-5 7 0.0 0.0 0.0 0.0 5.755041272278484E-5 8 0.0 0.0 0.0 0.0 5.755041272278484E-5 9 0.0 0.0 0.0 0.0 5.755041272278484E-5 10-11 0.0 0.0 0.0 0.0 5.755041272278484E-5 12-13 0.0 0.0 0.0 2.877520636139242E-5 2.877520636139242E-4 14-15 0.0 0.0 0.0 1.1510082544556968E-4 4.0285288905949387E-4 16-17 0.0 0.0 0.0 2.3020165089113935E-4 5.179537145050635E-4 18-19 0.0 0.0 0.0 4.0285288905949387E-4 5.179537145050635E-4 20-21 0.0 0.0 0.0 6.906049526734181E-4 5.179537145050635E-4 22-23 0.0 0.0 0.0 0.0017265123816835454 5.755041272278484E-4 24-25 2.877520636139242E-5 0.0 0.0 0.0038558776524265843 5.755041272278484E-4 26-27 5.755041272278484E-5 0.0 0.0 0.007999507368467093 5.755041272278484E-4 28-29 5.755041272278484E-5 0.0 0.0 0.019912442802083555 6.042793335892408E-4 30-31 5.755041272278484E-5 0.0 0.0 0.05334923259402155 6.330545399506332E-4 32-33 5.755041272278484E-5 0.0 0.0 0.11167657588856399 6.330545399506332E-4 34-35 5.755041272278484E-5 0.0 0.0 0.1913551223032596 6.330545399506332E-4 36-37 5.755041272278484E-5 0.0 0.0 0.3158654402290046 6.330545399506332E-4 38-39 5.755041272278484E-5 0.0 0.0 0.511968471581894 6.330545399506332E-4 40-41 5.755041272278484E-5 0.0 0.0 0.7326167539610511 6.330545399506332E-4 42-43 5.755041272278484E-5 0.0 0.0 0.9511068958631037 6.330545399506332E-4 44-45 5.755041272278484E-5 0.0 0.0 1.1982283680947416 6.330545399506332E-4 46-47 5.755041272278484E-5 0.0 0.0 1.485174725930547 6.330545399506332E-4 48-49 5.755041272278484E-5 0.0 0.0 1.7884941761859845 6.330545399506332E-4 50-51 5.755041272278484E-5 0.0 0.0 2.1374511037305903 6.330545399506332E-4 52-53 5.755041272278484E-5 0.0 0.0 2.455676110881229 6.330545399506332E-4 54-55 5.755041272278484E-5 0.0 0.0 2.7781310733669926 6.330545399506332E-4 56-57 5.755041272278484E-5 0.0 0.0 3.1548848502567037 6.330545399506332E-4 58-59 5.755041272278484E-5 0.0 0.0 3.562169121095852 6.618297463120257E-4 60-61 5.755041272278484E-5 0.0 0.0 4.008904199856469 6.906049526734181E-4 62-63 5.755041272278484E-5 0.0 0.0 4.4327342143534185 6.906049526734181E-4 64-65 5.755041272278484E-5 0.0 0.0 4.8450253710994495 7.481553653962029E-4 66-67 5.755041272278484E-5 0.0 0.0 5.300651988625736 7.481553653962029E-4 68-69 5.755041272278484E-5 0.0 0.0 5.805426658617282 7.481553653962029E-4 70-71 5.755041272278484E-5 0.0 0.0 6.347378895227747 7.481553653962029E-4 72-73 5.755041272278484E-5 0.0 0.0 6.888093797964672 7.481553653962029E-4 74-75 5.755041272278484E-5 0.0 0.0 7.392810917543495 7.481553653962029E-4 76-77 5.755041272278484E-5 0.0 0.0 7.921037380719576 7.481553653962029E-4 78-79 5.755041272278484E-5 0.0 0.0 8.502152673187897 7.481553653962029E-4 80-81 5.755041272278484E-5 0.0 0.0 9.09949718204404 7.481553653962029E-4 82-83 5.755041272278484E-5 0.0 0.0 9.657419658185079 7.481553653962029E-4 84-85 5.755041272278484E-5 0.0 0.0 10.182682275105936 7.481553653962029E-4 86-87 5.755041272278484E-5 0.0 0.0 10.700578439198278 7.769305717575954E-4 88-89 5.755041272278484E-5 0.0 0.0 11.251019361685351 8.057057781189877E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9875 0.0 37.571712 1 GTATCAA 16095 0.0 36.258137 1 TATCAAC 19770 0.0 30.478136 2 ATCAACG 20325 0.0 29.553371 3 TCAACGC 20600 0.0 29.274008 4 CAACGCA 20860 0.0 28.954626 5 AACGCAG 21345 0.0 28.341995 6 TACATGG 14145 0.0 26.59877 2 GTACATG 13855 0.0 26.436388 1 ACGCAGA 23630 0.0 25.480871 7 TATCCCG 150 1.0711101E-6 25.304783 5 CGCAGAG 23875 0.0 25.137724 8 ACATGGG 14635 0.0 24.671513 3 GAGTACT 12110 0.0 23.87582 12-13 GCAGAGT 23835 0.0 23.746746 9 ATGGGAG 3450 0.0 23.654469 5 CATGGGA 9990 0.0 23.462015 4 ATCCCGT 185 2.5702866E-7 23.082731 6 AGTACTT 12250 0.0 22.944628 12-13 GTACTTT 12840 0.0 22.37287 14-15 >>END_MODULE