FastQCFastQC Report
Fri 27 May 2016
SRR939895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737607
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163760.9424455587483246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134750.7754918114395257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97080.5586994067127952No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39080.22490701292064316No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30180.17368714559736464No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28930.16649334400701654No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23630.13599162526394057No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17410.10019526855036842No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94900.042.202091
GTATCAA154300.035.7083171
TATCAAC198800.027.6139812
ATCAACG201300.027.1765373
TCAACGC203050.026.9183124
CAACGCA207000.026.4046555
AACGCAG211000.025.9293796
GTACATG143650.025.1544951
TACATGG145250.024.7093282
ACATGGG146050.023.604553
TAAGGTA4800.023.5796534
CGCAGAG238050.022.90388
ACGCAGA238950.022.8766967
GAGTACT126750.022.7908812-13
TACGCGG652.4984224E-422.4604964-65
CATGGGA99150.022.4500184
AGTACTT129150.022.34911312-13
ATGGGAG33400.021.8853075
GCAGAGT245850.021.6787749
GTACTTT133350.021.41580814-15
Produced by FastQC (version 0.11.5)