FastQCFastQC Report
Fri 27 May 2016
SRR939894_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939894_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795066
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151900.8462084402467653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112800.6283891511509883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77250.4303462936738816No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32750.1824445452144935No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26750.14901959036603668No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25900.14428438842917196No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19820.11041376751606904No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18030.10044198931961276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA157050.039.715611
GGTATCA90400.038.8694041
TATCAAC195950.031.496952
TAACGCG600.004141455531.3644125
ATCAACG202450.030.4176753
TCAACGC205750.029.929814
CAACGCA207600.029.6420865
AACGCAG212350.029.1554586
GTACATG147500.027.457871
TACATGG147150.027.3968412
ACGCAGA236150.026.0377777
CGCAGAG238150.025.8191098
ACATGGG155300.024.8397123
GCAGAGT237000.024.3563679
CATGGGA105050.024.1825374
GAGTACT119450.023.82721912-13
ATGGGAG37050.023.1106195
AGTACTT122100.022.86712-13
AGAGTAC199850.022.00957310-11
GTACTTT129250.022.00791714-15
Produced by FastQC (version 0.11.5)