FastQCFastQC Report
Fri 27 May 2016
SRR939894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939894_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795066
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168700.9397983138224444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140100.7804726957114668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98970.5513446302252953No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39570.22043757722557278No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31770.1769851359225789No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29520.1644507778544076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23660.13180573861908143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103000.041.6990781
GTATCAA167300.036.7073861
TATCAAC212800.028.807742
ATCAACG214200.028.5474323
TCAACGC216950.028.141844
CAACGCA219800.027.7769455
AACGCAG225200.027.110896
GTACATG149850.024.956551
TACATGG151250.024.5004432
ACGCAGA249450.024.4182837
CGCAGAG251650.024.1859598
GAGTACT132800.024.05942312-13
ACATGGG155300.022.8488183
GCAGAGT260200.022.8260449
AGTACTT134850.022.70863312-13
GTACTTT140800.022.59133114-15
AGAGTAC220150.021.57024410-11
CATGGGA109500.021.271644
ACTTTTT155350.020.7044716-17
TAGACGC1150.00375200620.6255594
Produced by FastQC (version 0.11.5)