##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939894_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1795066 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.367094023283823 31.0 31.0 34.0 30.0 34.0 2 31.571078723567823 31.0 31.0 34.0 30.0 34.0 3 31.583173543479738 31.0 31.0 34.0 30.0 34.0 4 35.42444567497797 37.0 35.0 37.0 33.0 37.0 5 35.17374291530228 37.0 35.0 37.0 32.0 37.0 6 35.21810340121199 37.0 35.0 37.0 32.0 37.0 7 35.12299770593393 37.0 35.0 37.0 32.0 37.0 8 35.08667703583044 37.0 35.0 37.0 32.0 37.0 9 36.62476477188025 39.0 35.0 39.0 32.0 39.0 10-11 36.50026099318911 38.0 35.0 39.0 32.0 39.0 12-13 36.40735438139879 38.0 35.0 39.0 32.0 39.0 14-15 37.314666424521434 39.0 36.0 41.0 32.0 41.0 16-17 37.26920486489076 39.0 36.0 40.5 32.0 41.0 18-19 37.35804365967603 39.0 36.0 40.5 32.0 41.0 20-21 37.3201709575024 39.0 36.0 41.0 31.5 41.0 22-23 37.19127318995513 39.0 36.0 40.0 31.0 41.0 24-25 37.012729336971454 39.0 36.0 40.0 31.0 41.0 26-27 36.686704277168644 38.5 35.0 40.0 30.0 41.0 28-29 36.43318240109278 38.0 35.0 40.0 30.0 41.0 30-31 36.076262655523536 38.0 35.0 40.0 30.0 41.0 32-33 35.89057561114745 38.0 34.5 40.0 29.5 41.0 34-35 35.65767748929566 38.0 34.0 40.0 29.0 41.0 36-37 35.56917572947178 38.0 34.0 40.0 28.0 41.0 38-39 35.381914926804924 38.0 34.0 40.0 27.0 41.0 40-41 35.212727832848486 38.0 34.0 40.0 27.0 41.0 42-43 34.85653591567107 38.0 33.5 40.0 25.5 41.0 44-45 34.43416620893048 38.0 33.0 40.0 24.0 41.0 46-47 33.92752299915435 38.0 33.0 40.0 22.5 41.0 48-49 33.44336614921123 37.5 33.0 40.0 19.5 41.0 50-51 33.1233759650063 37.0 32.0 40.0 18.0 41.0 52-53 32.82914555787921 37.0 32.0 40.0 15.5 41.0 54-55 32.53036629293853 36.5 31.0 40.0 13.5 41.0 56-57 32.13561256243503 36.0 31.0 39.5 10.5 41.0 58-59 31.744709386730072 35.5 31.0 39.0 8.0 40.5 60-61 31.39975521791399 35.0 30.0 39.0 7.0 40.0 62-63 31.08523614173518 35.0 30.0 39.0 4.0 40.0 64-65 30.64263625961385 35.0 29.0 38.0 2.0 40.0 66-67 29.75080024912733 34.5 28.0 37.5 2.0 40.0 68-69 27.924967661356185 33.0 23.5 36.5 2.0 39.0 70-71 27.286459662207406 33.0 22.0 36.0 2.0 39.0 72-73 27.807525182918063 33.0 25.5 36.0 2.0 39.0 74-75 27.886401669910747 33.0 26.0 35.0 2.0 38.0 76-77 27.422622622232275 32.5 25.5 35.0 2.0 37.0 78-79 27.91691475410932 33.0 26.0 35.0 2.0 37.0 80-81 27.725130440886296 33.0 26.0 35.0 2.0 37.0 82-83 27.28882893442358 33.0 26.0 35.0 2.0 36.0 84-85 26.932332850157042 33.0 25.0 35.0 2.0 36.0 86-87 26.568093039475986 33.0 25.0 35.0 2.0 35.0 88-89 26.10959402049841 32.0 24.0 35.0 2.0 35.0 90-91 25.612584996874766 32.0 22.0 34.0 2.0 35.0 92-93 25.131919662006858 32.0 19.5 34.0 2.0 35.0 94-95 24.35558664695337 31.0 15.5 34.0 2.0 35.0 96-97 23.832082775786517 31.0 4.5 34.0 2.0 35.0 98-99 23.183333649013463 31.0 2.0 34.0 2.0 35.0 100-101 21.79216808741294 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2478209822012154 1101 2 0.25326767205296363 1101 3 0.37279744574627216 1101 4 0.3189894316093671 1101 5 0.4051248694694536 1101 6 0.2511864046109409 1101 7 0.18073355208339592 1101 8 0.23542553474869976 1101 9 0.3224441208895641 1101 10-11 0.4047165590878379 1101 12-13 0.4110187568461541 1101 14-15 0.42565651105232405 1101 16-17 0.5717962241865493 1101 18-19 0.37648210521528114 1101 20-21 0.5421307668107644 1101 22-23 0.37660900625984794 1101 24-25 0.31031998042873 1101 26-27 0.2600810834997347 1101 28-29 0.24539447570733586 1101 30-31 0.2704113710339655 1101 32-33 0.18543456117695456 1101 34-35 0.26146251924711095 1101 36-37 0.3203326624937759 1101 38-39 0.5028106720024326 1101 40-41 0.4922906175454784 1101 42-43 0.3948266427956497 1101 44-45 0.10388841028457563 1101 46-47 0.12709241052972686 1101 48-49 0.2917200854833055 1101 50-51 0.14793939603409 1101 52-53 -0.12466188160006908 1101 54-55 0.0586476757091674 1101 56-57 0.030109079871152744 1101 58-59 -0.0048245699866313885 1101 60-61 -0.1371946382958633 1101 62-63 -0.17645383400084924 1101 64-65 -0.3906228489360011 1101 66-67 -0.37544908816909484 1101 68-69 -8.311133435292671 1101 70-71 -6.408808646582095 1101 72-73 -1.6261637759948258 1101 74-75 -0.8033978941730453 1101 76-77 -0.49250121158327786 1101 78-79 -0.5456347317730241 1101 80-81 -0.5520381756010515 1101 82-83 -0.3991018964211328 1101 84-85 -0.3878041165017798 1101 86-87 -0.5611752362092481 1101 88-89 -0.4211800788173399 1101 90-91 -0.7509910386576522 1101 92-93 -1.0372002538722676 1101 94-95 -0.5531526010311616 1101 96-97 -1.0639255347259926 1101 98-99 -1.0068140030643882 1101 100-101 -1.0234613675097073 1102 1 0.19385896267711544 1102 2 0.12108722665943361 1102 3 0.2788567807066116 1102 4 0.15126648062299353 1102 5 0.2075704663781366 1102 6 0.09092099887573823 1102 7 0.21682771519098054 1102 8 0.20578093438262357 1102 9 0.30141503811551473 1102 10-11 0.3293536599623579 1102 12-13 0.2724955211333224 1102 14-15 0.2702155663706094 1102 16-17 0.32750981987497596 1102 18-19 0.38856454877842594 1102 20-21 0.2935823633126873 1102 22-23 0.2770983798616342 1102 24-25 0.2758680788627288 1102 26-27 0.26113927446983354 1102 28-29 0.2629121440010067 1102 30-31 0.17897795126581428 1102 32-33 0.2029465095194425 1102 34-35 -0.1300071268785743 1102 36-37 0.0036837912308058662 1102 38-39 0.18567814708887198 1102 40-41 0.036555413152555616 1102 42-43 0.14903398467077977 1102 44-45 -0.009017706249899504 1102 46-47 0.01315225393017272 1102 48-49 0.12186232160289734 1102 50-51 -0.007196484584170548 1102 52-53 -0.08684664873450743 1102 54-55 -0.46409993462233246 1102 56-57 -0.5128153405071316 1102 58-59 -0.4664062000456113 1102 60-61 -0.17877677679494397 1102 62-63 -0.24340904042752953 1102 64-65 -0.028397406593107632 1102 66-67 0.1434871280196397 1102 68-69 -4.290162823130782 1102 70-71 -0.08519986582146544 1102 72-73 0.27107285203039666 1102 74-75 0.18901363724855358 1102 76-77 0.014043683678064411 1102 78-79 -0.10123519953792126 1102 80-81 -0.0658054625646507 1102 82-83 -0.15311539550593878 1102 84-85 -0.3553904242097268 1102 86-87 -0.424102961448213 1102 88-89 -0.3895926016971494 1102 90-91 -0.5034150268618411 1102 92-93 -0.66611969153751 1102 94-95 -0.32657453005292325 1102 96-97 -0.8544475756045777 1102 98-99 -0.7578861761374007 1102 100-101 -0.40055359854489225 1103 1 0.20251187362516987 1103 2 0.22985044172637714 1103 3 0.27036224560493594 1103 4 0.2659150663577421 1103 5 0.3774950173670888 1103 6 0.24571848274934638 1103 7 0.27879397306163867 1103 8 0.3658057528093721 1103 9 0.5212503396569161 1103 10-11 0.48594715698799007 1103 12-13 0.4132722397596851 1103 14-15 0.5319057223490802 1103 16-17 0.3681167658048423 1103 18-19 0.4341730660451262 1103 20-21 0.3909729504461197 1103 22-23 0.46554917838643917 1103 24-25 0.30014507759696585 1103 26-27 0.4100190914246227 1103 28-29 0.25451015008015787 1103 30-31 0.2167645649666312 1103 32-33 0.20823471008413463 1103 34-35 0.05213720755158846 1103 36-37 0.40758784900631184 1103 38-39 0.4627465977507583 1103 40-41 0.3653762770275861 1103 42-43 0.3473814103490298 1103 44-45 0.522536352084586 1103 46-47 0.3809548480644267 1103 48-49 0.3445690393473271 1103 50-51 0.15596665992788417 1103 52-53 0.24451117882091467 1103 54-55 0.18640245840565228 1103 56-57 0.4607289059778594 1103 58-59 0.15773142794380846 1103 60-61 0.1529060317864186 1103 62-63 0.18089025514154855 1103 64-65 0.08799067385436743 1103 66-67 0.18182417542184126 1103 68-69 -3.914095804683413 1103 70-71 0.33384647557226543 1103 72-73 0.7089600188666161 1103 74-75 0.576930584954713 1103 76-77 0.2876168415185383 1103 78-79 0.15648593351393814 1103 80-81 0.2943120212592767 1103 82-83 0.048779804401643645 1103 84-85 0.12913454762421495 1103 86-87 -0.2043398606603084 1103 88-89 -0.09237237728789083 1103 90-91 -0.004385932908320456 1103 92-93 -0.014233373098957003 1103 94-95 -0.18959494997871573 1103 96-97 -0.5677644689039738 1103 98-99 -0.13975797989544603 1103 100-101 -0.35803496298697013 1104 1 0.12531329815903547 1104 2 0.1525651756763331 1104 3 0.3008782468857589 1104 4 0.1601024932316406 1104 5 0.29506814499546863 1104 6 0.24208175170474533 1104 7 0.13959331518999818 1104 8 0.20355425182601294 1104 9 0.3026255392512951 1104 10-11 0.2443226426002596 1104 12-13 0.2929084689756891 1104 14-15 0.23208908514398274 1104 16-17 0.34963213552644845 1104 18-19 0.23105489121157774 1104 20-21 0.21974271643343712 1104 22-23 0.2168749490855788 1104 24-25 0.20219738559640632 1104 26-27 0.33700974685122276 1104 28-29 0.4364820800353826 1104 30-31 0.4754495725932628 1104 32-33 0.429433824008683 1104 34-35 0.41062383415540893 1104 36-37 0.3994789216771366 1104 38-39 0.5716682798289199 1104 40-41 0.5418583120829368 1104 42-43 0.4419648056223764 1104 44-45 0.5704086324346136 1104 46-47 0.41594274038128987 1104 48-49 0.5464590467007611 1104 50-51 0.5145636338770601 1104 52-53 0.40040496331971553 1104 54-55 0.4730886772584739 1104 56-57 0.4649598032956135 1104 58-59 0.5148061274495177 1104 60-61 0.5995115001918201 1104 62-63 0.6568977202997175 1104 64-65 0.47006171800887486 1104 66-67 0.5930111984519293 1104 68-69 -3.7040547457029867 1104 70-71 0.8114583270297828 1104 72-73 1.0854966018351497 1104 74-75 0.9795279142131825 1104 76-77 0.8964965519231463 1104 78-79 0.4612390500251671 1104 80-81 0.5483831972811508 1104 82-83 0.5118108490933864 1104 84-85 0.3849789123852254 1104 86-87 0.4157749799434818 1104 88-89 0.46574221240160796 1104 90-91 0.50308868796537 1104 92-93 0.2830901466974787 1104 94-95 0.4192159268812752 1104 96-97 0.07642333953059222 1104 98-99 0.5036783066462398 1104 100-101 0.5568768552900316 1105 1 0.14154634559121249 1105 2 0.21107285805937082 1105 3 0.1316804834402845 1105 4 0.11443011897078748 1105 5 0.24454960462589526 1105 6 0.2169599941650162 1105 7 0.15542559445836446 1105 8 0.2455419427309593 1105 9 0.19575035395189389 1105 10-11 0.24094084462614518 1105 12-13 0.19293394531622 1105 14-15 0.3110046571474214 1105 16-17 0.31396332199388866 1105 18-19 0.2564625499060895 1105 20-21 0.3157973102943785 1105 22-23 0.2842817704874889 1105 24-25 0.31242025143143337 1105 26-27 0.3344582392031086 1105 28-29 0.381860507981294 1105 30-31 0.6125222665747359 1105 32-33 0.5574335141210298 1105 34-35 0.22147884100124315 1105 36-37 0.2098159095989658 1105 38-39 0.49685246185214993 1105 40-41 0.3479693561510473 1105 42-43 0.45677847822310014 1105 44-45 0.5574507101121213 1105 46-47 0.5280578576841961 1105 48-49 0.3001989287944724 1105 50-51 0.24906113168326272 1105 52-53 0.23313252588945943 1105 54-55 0.23717687009202137 1105 56-57 0.1052222542983543 1105 58-59 0.03764201661105204 1105 60-61 0.1908318152641293 1105 62-63 0.25305928499397723 1105 64-65 0.3317256359124876 1105 66-67 0.7677550569405405 1105 68-69 1.9026415092749573 1105 70-71 2.246196434718719 1105 72-73 1.2920519175257468 1105 74-75 0.8801910439890612 1105 76-77 0.3514920749404986 1105 78-79 0.49873888241766196 1105 80-81 0.5926510951552899 1105 82-83 0.4825091838942157 1105 84-85 0.3933850618672814 1105 86-87 0.4917911656138614 1105 88-89 0.5542780081498897 1105 90-91 0.7225107474965746 1105 92-93 0.34103869044899326 1105 94-95 0.8433405315137996 1105 96-97 0.3796373810168028 1105 98-99 0.6688257789016099 1105 100-101 0.6879115075826796 1106 1 0.36940283144536323 1106 2 0.28420963726315307 1106 3 0.37271331859779266 1106 4 0.21590520091978505 1106 5 0.28571608918367986 1106 6 0.2841797779801709 1106 7 0.33575011866032156 1106 8 0.37795763249150127 1106 9 0.4800751003884258 1106 10-11 0.45635574421933 1106 12-13 0.4103418568668644 1106 14-15 0.5879513702364179 1106 16-17 0.5743006246835449 1106 18-19 0.5541502300858525 1106 20-21 0.5105170786765711 1106 22-23 0.5800685734580213 1106 24-25 0.5815614382874941 1106 26-27 0.628988336554194 1106 28-29 0.5399501549369816 1106 30-31 0.81137158536405 1106 32-33 0.793569009302864 1106 34-35 0.7513297277789022 1106 36-37 0.6429952220299029 1106 38-39 0.7519998157174896 1106 40-41 0.5451587061766645 1106 42-43 0.3957196848333382 1106 44-45 0.5032030975904362 1106 46-47 0.5306658729443043 1106 48-49 0.6012063921720667 1106 50-51 0.6463027994302379 1106 52-53 0.7559422807523859 1106 54-55 0.7282842464277479 1106 56-57 0.707033003043648 1106 58-59 0.691018901746645 1106 60-61 0.8217907882934945 1106 62-63 0.9303811732470315 1106 64-65 0.9971774202708765 1106 66-67 1.4602279412365817 1106 68-69 2.637492056527581 1106 70-71 3.1068021165158584 1106 72-73 2.0786638280639878 1106 74-75 1.6275790161603574 1106 76-77 1.2118022156273263 1106 78-79 0.9307656616831785 1106 80-81 1.0124847587538852 1106 82-83 1.165588645098854 1106 84-85 1.2655512624586684 1106 86-87 1.2091155848717214 1106 88-89 1.2364851986728205 1106 90-91 0.9902092616115574 1106 92-93 1.0100669080660225 1106 94-95 1.36873793013471 1106 96-97 1.304847114890908 1106 98-99 1.5360526618765924 1106 100-101 1.5341566693509243 1107 1 0.12230338768932114 1107 2 0.20421834326015187 1107 3 0.07476726609964146 1107 4 0.11585486937464395 1107 5 0.07205064185125565 1107 6 0.21714978433653442 1107 7 0.0016964382742941098 1107 8 0.10330259525605356 1107 9 0.15963196019466608 1107 10-11 0.2223564144821708 1107 12-13 0.1602679671906415 1107 14-15 0.2645325474751843 1107 16-17 0.26688123553784493 1107 18-19 0.15297658996505703 1107 20-21 0.07472009659064582 1107 22-23 0.2589990714064285 1107 24-25 0.22771954611819467 1107 26-27 0.1045852662062785 1107 28-29 0.30800829714647904 1107 30-31 0.4117057274647209 1107 32-33 0.3312685114286609 1107 34-35 0.5345408185265583 1107 36-37 0.4553995364450074 1107 38-39 0.5468873302790129 1107 40-41 0.5226133892363265 1107 42-43 0.48120138368993537 1107 44-45 0.3905170130878517 1107 46-47 0.552397785122551 1107 48-49 0.5940338378276024 1107 50-51 0.5895323409556141 1107 52-53 0.6352519965955707 1107 54-55 0.5527696774858981 1107 56-57 0.6096924748963204 1107 58-59 0.7191312956732219 1107 60-61 0.8543594407683948 1107 62-63 0.9085914478074635 1107 64-65 0.9027896967159599 1107 66-67 1.3462112242383704 1107 68-69 2.4975688152255415 1107 70-71 2.8471868745905446 1107 72-73 1.9906201051620194 1107 74-75 1.397439880972545 1107 76-77 0.8883268162520785 1107 78-79 0.9380995985003935 1107 80-81 0.9800212212995483 1107 82-83 1.0027818638197132 1107 84-85 0.8510737706761908 1107 86-87 0.9759154384526809 1107 88-89 0.9278239064753215 1107 90-91 0.8555624851800729 1107 92-93 0.8557024087474154 1107 94-95 1.30897262105945 1107 96-97 1.119664128189811 1107 98-99 1.4191554841530625 1107 100-101 1.599430005503816 1108 1 -0.11562476952829925 1108 2 -0.0984758483601702 1108 3 0.008871814839128689 1108 4 -0.05029160220140483 1108 5 0.05494704622088875 1108 6 0.035584241500735914 1108 7 0.07621002095214635 1108 8 -0.07330363800780049 1108 9 -0.10665555756703071 1108 10-11 -0.04837981867695618 1108 12-13 -0.12475355631763563 1108 14-15 -0.022751300990940138 1108 16-17 -0.10453805916139913 1108 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0.5056637050347845 2316 74-75 -0.03138046125135574 2316 76-77 -0.3646026473815205 2316 78-79 -0.40131035048056063 2316 80-81 -0.6334028658753184 2316 82-83 -0.462734075412353 2316 84-85 -0.3904390480595801 2316 86-87 -0.323523814049846 2316 88-89 -0.33318179607172027 2316 90-91 -0.3716926331624357 2316 92-93 -0.24813628149712486 2316 94-95 -0.1349588488613449 2316 96-97 -0.13344659434013906 2316 98-99 -0.2122820943702486 2316 100-101 -0.4484396693497139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 22.0 4 48.0 5 120.0 6 310.0 7 761.0 8 1715.0 9 3007.0 10 4975.0 11 7869.0 12 10574.0 13 13379.0 14 16819.0 15 21323.0 16 26318.0 17 30985.0 18 26555.0 19 18723.0 20 16993.0 21 17246.0 22 18141.0 23 20265.0 24 23847.0 25 28123.0 26 33735.0 27 41490.0 28 49947.0 29 59795.0 30 70162.0 31 83568.0 32 98725.0 33 118790.0 34 147880.0 35 186702.0 36 225025.0 37 234773.0 38 126424.0 39 9931.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.21050872012036 19.50632417674263 15.312141304953474 20.97102579818354 2 16.06157679798199 21.627103419800218 39.30750341155343 23.003816370664374 3 19.427865047858965 27.021903372912192 30.491747935730494 23.058483643498345 4 12.903480986214436 17.706089915356873 36.778202027112094 32.612227071316596 5 12.69535493402471 39.40334227265181 32.77261114633111 15.128691646992367 6 30.038616964501585 37.182699689036504 17.233795303348177 15.544888043113737 7 27.229639467295353 35.38633119896427 19.722283191815787 17.661746141924585 8 27.45297387394113 33.57776260037235 19.03434191277647 19.934921612910056 9 28.006379709715407 16.17650827323341 19.657940153732508 36.159171863318676 10-11 25.195257444573066 27.481691480981755 25.748106197766546 21.57494487667863 12-13 27.20393010619108 25.16319734204759 26.773806645549524 20.859065906211804 14-15 23.52434395169871 25.61588264721186 27.072319346475282 23.787454054614148 16-17 21.081909331063397 29.100313193222725 27.37151111551827 22.446266360195605 18-19 21.098862103120442 26.392762160277115 31.611651047928042 20.8967246886744 20-21 22.75551428192612 24.997688107289648 31.703653236148423 20.54314437463581 22-23 21.959498982210125 25.498031827242006 31.713485743699678 20.82898344684819 24-25 21.495863100145733 25.84150203001119 31.883180766813883 20.779454103029195 26-27 20.964713465248963 26.049497468471134 31.920243401131383 21.06554566514852 28-29 20.92101477190083 26.62643967748216 31.653337054650155 20.799208495966855 30-31 21.07613763005495 26.19786486067167 31.4483065593684 21.277690949904976 32-33 20.720540541895645 26.160165369229816 31.476838475030966 21.642455613843573 34-35 21.4732118980978 26.200768143975182 31.363121289643985 20.96289866828303 36-37 21.48239398525759 25.867502344977733 31.362920018692904 21.287183651071775 38-39 21.041580526740017 26.271369555828848 31.174451857318548 21.51259806011259 40-41 21.332789418078526 26.286414880004504 31.207249278722088 21.173546423194882 42-43 21.037474503702278 26.304092931303945 30.723404729937915 21.93502783505586 44-45 21.140500218317843 26.316361793094913 30.8124267742995 21.730711214287744 46-47 21.703755271802965 26.727689055141795 29.825349875974798 21.74320579708044 48-49 21.306482029801078 27.026394060750835 29.49494746726532 22.172176442182764 50-51 21.395881804355735 27.28450288769964 29.34923252568819 21.970382782256433 52-53 21.82137116983219 27.166059095092805 28.880174695178212 22.132395039896796 54-55 21.235039995540568 27.239889629030912 29.084840714624153 22.44022966080437 56-57 21.57889002789433 27.26567269478874 28.592374100037866 22.563063177279062 58-59 21.519591046480855 26.893374005822295 28.765784965108022 22.82124998258883 60-61 21.2242836556689 27.18418826287562 29.106418232380786 22.485109849074693 62-63 21.2799086810139 27.231408561377783 28.983861798140587 22.50482095946773 64-65 20.950296299061215 27.44790044663525 28.814714617513204 22.78708863679033 66-67 20.904071274999993 27.304694376105232 28.821438962956226 22.969795385938546 68-69 21.10059822660451 27.062810577051355 29.024772326236974 22.811818870107157 70-71 21.02327797722482 27.198505144577823 28.636816910971426 23.141399967225933 72-73 20.976744718177706 27.31434547762496 28.548279844706208 23.160629959491125 74-75 21.08254527531577 27.87977031548604 27.844719716407777 23.192964692790415 76-77 20.919255260637044 27.56977264507745 28.07205544475446 23.43891664953104 78-79 21.121340898151285 27.67947872283529 27.935108881678637 23.264071497334786 80-81 21.136538297089448 27.75564423827121 27.690520286890784 23.417297177748555 82-83 21.099180252525674 27.78943937963249 27.599446259606196 23.511934108235636 84-85 20.992230330290347 27.81680470313756 27.4834726696701 23.707492296901993 86-87 21.005299582685023 27.8466803376179 27.19544390759685 23.95257617210023 88-89 20.929873692314636 27.871838338641417 26.96022586419069 24.23806210485326 90-91 20.77946314766782 27.80687622001765 27.107288017755437 24.3063726145591 92-93 20.857800310469816 27.674125351701917 27.256888009419384 24.21118632840888 94-95 20.4907679943758 27.425650694926656 27.574032916155268 24.50954839454228 96-97 20.650343402556164 27.17706745667038 27.613922645346072 24.55866649542738 98-99 20.628103520517783 27.293763539595794 27.156835322855283 24.92129761703114 100-101 20.361488139079583 27.27950410221039 27.388647524132452 24.97036023457757 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2570.0 1 1691.5 2 1001.0 3 1280.0 4 1172.5 5 1151.5 6 1880.0 7 3644.5 8 5525.0 9 6246.0 10 5223.0 11 3910.0 12 3652.5 13 3678.5 14 3920.5 15 4148.5 16 4360.5 17 4699.5 18 5007.5 19 5449.0 20 6006.0 21 6679.0 22 7462.0 23 8473.5 24 9617.0 25 11200.5 26 13493.5 27 15975.0 28 18028.0 29 19948.5 30 22326.5 31 25498.5 32 29932.5 33 32040.0 34 32860.0 35 35299.0 36 38446.0 37 41934.5 38 43855.0 39 44956.0 40 46797.5 41 49109.0 42 51146.5 43 53706.0 44 56368.5 45 58821.5 46 61650.5 47 65401.0 48 69360.0 49 68320.0 50 63958.5 51 60706.0 52 58177.0 53 57570.5 54 57392.0 55 54486.5 56 50669.0 57 46789.0 58 44055.0 59 40280.0 60 33530.0 61 27511.5 62 22521.5 63 16591.5 64 11436.5 65 8629.0 66 6608.5 67 5138.0 68 3815.0 69 2664.5 70 1993.5 71 1590.0 72 1265.5 73 1141.5 74 913.0 75 634.0 76 470.5 77 330.0 78 217.5 79 127.5 80 83.0 81 51.0 82 29.5 83 18.5 84 11.5 85 8.0 86 5.0 87 4.0 88 3.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020389222457558663 2 0.02389884271664663 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.4566606464609105E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.1141651616152276E-4 26-27 2.506871613634262E-4 28-29 1.3927064520190345E-4 30-31 4.178119356057103E-4 32-33 0.002924683549239972 34-35 0.005013743227268524 36-37 0.10428585912718528 38-39 0.09551180847946537 40-41 0.10553929493400242 42-43 0.10659775183753689 44-45 0.10077623886809732 46-47 0.09361772770471949 48-49 0.09514970480194042 50-51 0.09378485247896177 52-53 0.07314494286003968 54-55 0.0621704160181297 56-57 0.034288432848708625 58-59 0.013425690197463492 60-61 0.009553966260850576 62-63 0.0022004761941900744 64-65 0.004958034969187762 66-67 0.15266848127032656 68-69 0.4103191748938479 70-71 0.39369025985674067 72-73 0.1802997772783842 74-75 0.028829023556794012 76-77 0.03114091626714561 78-79 0.005013743227268524 80-81 0.0015319770972209376 82-83 5.570825808076138E-5 84-85 5.013743227268524E-4 86-87 0.0010584569035344661 88-89 2.785412904038069E-4 90-91 5.570825808076138E-4 92-93 0.002479017484593881 94-95 0.07729520808705641 96-97 0.01707458110175336 98-99 0.018383725166651252 100-101 0.010027486454537047 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1795066.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.85341409975476 #Duplication Level Percentage of deduplicated Percentage of total 1 69.92124558524525 31.36206582605654 2 14.151382910869618 12.694756755708555 3 6.024651064465105 8.10678507002945 4 3.117209612078841 5.592699742652326 5 1.8279426116210875 4.0994733454813925 6 1.205265418002316 3.2436161336263134 7 0.7957694505268003 2.4985083684689013 8 0.5652077170024109 2.028119662646891 9 0.39024774882046726 1.575354949140735 >10 1.8209865717261753 14.025176405791731 >50 0.10844653768388172 3.358158358941636 >100 0.0630628075611744 5.364012087176642 >500 0.00522380441542267 1.6582132311783313 >1k 0.0029850310945272397 2.118232956124386 >5k 1.2437629560530165E-4 0.5521240865621028 >10k+ 2.487525912106033E-4 1.7227030204140383 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16870 0.9397983138224444 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14010 0.7804726957114668 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9897 0.5513446302252953 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3957 0.22043757722557278 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3177 0.1769851359225789 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2952 0.1644507778544076 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2366 0.13180573861908143 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.7854129040380687E-5 1.1141651616152275E-4 14-15 0.0 0.0 0.0 5.5708258080761375E-5 1.6712477424228413E-4 16-17 0.0 0.0 0.0 1.3927064520190345E-4 1.6712477424228413E-4 18-19 0.0 0.0 0.0 2.506871613634262E-4 1.6712477424228413E-4 20-21 0.0 0.0 0.0 5.570825808076138E-4 2.785412904038069E-4 22-23 0.0 0.0 0.0 0.0013648523229786535 2.785412904038069E-4 24-25 0.0 0.0 0.0 0.003704599162370631 2.785412904038069E-4 26-27 0.0 0.0 0.0 0.00768773961514507 3.063954194441876E-4 28-29 0.0 0.0 0.0 0.018133038005287826 3.3424954848456826E-4 30-31 0.0 0.0 0.0 0.0513351598214216 3.3424954848456826E-4 32-33 0.0 0.0 0.0 0.10824114545091937 3.3424954848456826E-4 34-35 0.0 0.0 0.0 0.18896241140994258 3.3424954848456826E-4 36-37 0.0 0.0 0.0 0.31828913254443014 3.3424954848456826E-4 38-39 0.0 0.0 0.0 0.5190338405384538 4.45666064646091E-4 40-41 0.0 0.0 0.0 0.7534263364132572 4.45666064646091E-4 42-43 0.0 0.0 0.0 0.9807438835118041 4.7352019368647165E-4 44-45 0.0 0.0 0.0 1.2327123348110878 5.292284517672331E-4 46-47 0.0 0.0 0.0 1.5309186403174033 5.570825808076138E-4 48-49 0.0 0.0 0.0 1.8522717270562754 6.127908388883751E-4 50-51 0.0 0.0 0.0 2.21184067883855 6.684990969691365E-4 52-53 0.0 0.0 0.0 2.5353942417716118 7.242073550498978E-4 54-55 0.0 0.0 0.0 2.8709250801920376 7.242073550498978E-4 56-57 0.0 0.0 0.0 3.252498793916213 7.242073550498978E-4 58-59 0.0 0.0 0.0 3.649280862096435 7.242073550498978E-4 60-61 0.0 0.0 0.0 4.069488252799618 7.520614840902785E-4 62-63 0.0 0.0 0.0 4.466298175108882 7.799156131306593E-4 64-65 0.0 5.5708258080761375E-5 0.0 4.8559217321257275 7.799156131306593E-4 66-67 0.0 5.5708258080761375E-5 0.0 5.258246771984986 7.799156131306593E-4 68-69 0.0 5.5708258080761375E-5 0.0 5.720040377345457 7.799156131306593E-4 70-71 0.0 5.5708258080761375E-5 0.0 6.245062855627593 7.799156131306593E-4 72-73 0.0 5.5708258080761375E-5 0.0 6.789806057270317 8.0776974217104E-4 74-75 0.0 5.5708258080761375E-5 0.0 7.33513419562289 8.356238712114207E-4 76-77 0.0 5.5708258080761375E-5 0.0 7.8888464268166185 8.356238712114207E-4 78-79 0.0 5.5708258080761375E-5 0.0 8.513781665966599 8.356238712114207E-4 80-81 0.0 5.5708258080761375E-5 0.0 9.173451004029936 8.356238712114207E-4 82-83 0.0 5.5708258080761375E-5 0.0 9.852896773711942 8.91332129292182E-4 84-85 0.0 5.5708258080761375E-5 0.0 10.492789680156607 8.91332129292182E-4 86-87 0.0 5.5708258080761375E-5 0.0 11.119646854210373 8.91332129292182E-4 88-89 0.0 5.5708258080761375E-5 0.0 11.790624968664105 8.91332129292182E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10300 0.0 41.699078 1 GTATCAA 16730 0.0 36.707386 1 TATCAAC 21280 0.0 28.80774 2 ATCAACG 21420 0.0 28.547432 3 TCAACGC 21695 0.0 28.14184 4 CAACGCA 21980 0.0 27.776945 5 AACGCAG 22520 0.0 27.11089 6 GTACATG 14985 0.0 24.95655 1 TACATGG 15125 0.0 24.500443 2 ACGCAGA 24945 0.0 24.418283 7 CGCAGAG 25165 0.0 24.185959 8 GAGTACT 13280 0.0 24.059423 12-13 ACATGGG 15530 0.0 22.848818 3 GCAGAGT 26020 0.0 22.826044 9 AGTACTT 13485 0.0 22.708633 12-13 GTACTTT 14080 0.0 22.591331 14-15 AGAGTAC 22015 0.0 21.570244 10-11 CATGGGA 10950 0.0 21.27164 4 ACTTTTT 15535 0.0 20.70447 16-17 TAGACGC 115 0.003752006 20.625559 4 >>END_MODULE