FastQCFastQC Report
Fri 27 May 2016
SRR939893_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939893_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757189
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94470.537620028352101No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69790.39716843208101127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47140.2682693779667412No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22290.12685032742636107No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21200.12064723828797015No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20010.11387505840293786No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA19290.10977760502712001No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG18500.1052817881286532No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC17850.1015826982754843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42000.060.989741
GTATCAA86000.046.4436071
GTGGTAT16300.038.1237831
TATCAAC114050.036.311452
TGGTATC16800.035.578362
ATCAACG118250.035.1430973
TCAACGC119500.034.776484
CAACGCA121450.033.9818045
AACGCAG125400.032.9492346
ACGCAGA138250.029.4749267
CGCAGAG140650.028.9061688
TACATGG97500.026.954412
GCAGAGT142350.026.593939
GTACATG96500.026.3972851
AGGTAAG9150.024.8854122
TAAGGTG10550.024.7306145
GTAAGGT10850.024.4854284
ACATGGG104750.024.093213
GTATAGA4050.023.4254151
GAGTACT86750.023.13073212-13
Produced by FastQC (version 0.11.5)