##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939893_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1757189 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.740754124912005 31.0 28.0 33.0 16.0 34.0 2 29.54767700002675 31.0 30.0 33.0 25.0 34.0 3 29.78949617827109 31.0 30.0 33.0 25.0 34.0 4 33.589200706355435 35.0 33.0 37.0 28.0 37.0 5 33.54458683727248 35.0 33.0 37.0 28.0 37.0 6 33.58916257727541 35.0 33.0 37.0 28.0 37.0 7 33.506247762762 35.0 33.0 37.0 28.0 37.0 8 33.35988331363331 35.0 33.0 37.0 26.0 37.0 9 34.60218337355857 37.0 34.0 39.0 27.0 39.0 10-11 34.40776376360198 37.0 33.0 39.0 27.0 39.0 12-13 34.25958562226374 37.0 33.0 39.0 26.5 39.0 14-15 34.84102649174335 38.0 33.0 40.0 25.5 41.0 16-17 34.665339357348586 38.0 33.0 40.0 25.0 41.0 18-19 34.55294848761288 38.0 33.0 40.0 25.0 41.0 20-21 34.75552544433182 38.0 33.0 40.0 25.0 41.0 22-23 34.645556624813835 38.0 33.0 40.0 24.5 41.0 24-25 34.3953234398804 38.0 33.0 40.0 23.5 41.0 26-27 34.229031140076565 38.0 32.5 40.0 23.5 41.0 28-29 34.225108397559964 38.0 33.0 40.0 23.5 41.0 30-31 33.93865713932878 38.0 32.5 40.0 22.0 41.0 32-33 33.507745894152535 37.0 32.0 40.0 19.0 41.0 34-35 33.26166394167048 37.0 31.0 40.0 18.0 41.0 36-37 32.96772231103199 37.0 31.0 40.0 16.5 41.0 38-39 32.66718975591129 37.0 31.0 40.0 15.5 41.0 40-41 32.337347320066314 37.0 30.0 40.0 14.5 41.0 42-43 31.845600274074105 36.0 30.0 39.0 9.0 41.0 44-45 31.384964281019286 36.0 30.0 39.0 8.5 40.5 46-47 30.850191982763377 35.5 28.5 39.0 4.5 40.0 48-49 30.37481483209831 35.0 27.5 39.0 2.0 40.0 50-51 29.68043847303847 34.5 26.5 38.5 2.0 40.0 52-53 29.78664275726743 35.0 27.0 38.5 2.0 40.0 54-55 29.826059974197427 35.0 27.0 39.0 2.0 40.0 56-57 29.463935012113097 35.0 26.5 38.5 2.0 40.0 58-59 29.018408093836236 34.0 26.0 38.0 2.0 40.0 60-61 28.563517071868766 34.0 26.0 38.0 2.0 40.0 62-63 28.07342152722331 34.0 25.0 37.5 2.0 40.0 64-65 27.497711117017012 33.0 23.0 37.0 2.0 39.0 66-67 26.870737012353253 33.0 22.0 36.0 2.0 39.0 68-69 26.294429341408353 32.0 20.5 36.0 2.0 39.0 70-71 25.68357387850709 31.5 18.5 35.0 2.0 38.0 72-73 25.019934395218726 31.0 16.0 35.0 2.0 37.5 74-75 24.668412447380447 31.0 12.5 35.0 2.0 37.0 76-77 24.105042200924316 31.0 7.5 34.5 2.0 36.5 78-79 23.451111690319028 30.0 4.5 34.0 2.0 36.0 80-81 22.737494657660616 29.5 2.0 34.0 2.0 35.5 82-83 21.98261370859936 29.0 2.0 34.0 2.0 35.0 84-85 21.209708801955852 28.5 2.0 34.0 2.0 35.0 86-87 20.31648303056757 27.0 2.0 33.5 2.0 35.0 88-89 19.335209815221926 25.5 2.0 33.0 2.0 35.0 90-91 17.969859531331004 22.5 2.0 32.0 2.0 34.0 92-93 16.50255208745331 15.5 2.0 31.0 2.0 34.0 94-95 15.947287969592342 11.0 2.0 31.0 2.0 34.0 96-97 15.574763727749264 4.5 2.0 31.0 2.0 34.0 98-99 14.672631117085299 2.0 2.0 31.0 2.0 34.0 100-101 13.146370140036161 2.0 2.0 29.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6288722636528092 1101 2 0.7998427402197734 1101 3 0.8845576509799926 1101 4 0.6375149076238102 1101 5 0.903144592784507 1101 6 0.9749693207081052 1101 7 0.8031271653022074 1101 8 0.8760998538483733 1101 9 0.8393953511723069 1101 10-11 0.9337146394634388 1101 12-13 1.067482289607419 1101 14-15 1.3242148274506533 1101 16-17 1.0064330230141323 1101 18-19 0.8982412582060135 1101 20-21 0.8593824748449848 1101 22-23 1.0183400925004378 1101 24-25 1.2589595869344308 1101 26-27 1.287498978092529 1101 28-29 1.2086649627727013 1101 30-31 1.429991358787639 1101 32-33 1.5230933917221634 1101 34-35 1.4229343487130066 1101 36-37 1.4938214245939818 1101 38-39 1.2764875874034587 1101 40-41 1.363811502721866 1101 42-43 1.5335809298059395 1101 44-45 1.4370554852143052 1101 46-47 1.258883360517963 1101 48-49 1.3648290585366638 1101 50-51 1.0929205781244207 1101 52-53 1.0119140153883102 1101 54-55 1.1065189283569588 1101 56-57 1.2142299061818846 1101 58-59 0.8652504781050254 1101 60-61 0.6463352784311951 1101 62-63 0.3652326987110541 1101 64-65 0.6198828521046948 1101 66-67 0.6249110534341895 1101 68-69 0.3771586805295293 1101 70-71 0.41225394392722237 1101 72-73 0.22784825206679926 1101 74-75 0.16712571243022722 1101 76-77 -0.20395810905025868 1101 78-79 -0.09312835352287507 1101 80-81 -0.4612525398002312 1101 82-83 -0.47117539788609974 1101 84-85 -0.28218966864217165 1101 86-87 -0.9081289422845309 1101 88-89 -4.718256919290479 1101 90-91 -7.795546640314869 1101 92-93 -10.072625891853363 1101 94-95 -8.243038850358639 1101 96-97 -5.444509931584369 1101 98-99 -4.206478033016639 1101 100-101 -3.111542765949956 1102 1 1.9668626942748197 1102 2 1.4172887508236798 1102 3 1.227282337388889 1102 4 1.3310736817089008 1102 5 1.2260438556837698 1102 6 1.1611053610546733 1102 7 1.2375083886307934 1102 8 1.1446916034927526 1102 9 1.4460758115369785 1102 10-11 1.3481417699431901 1102 12-13 1.334439486038299 1102 14-15 1.663513935170819 1102 16-17 1.609243057929433 1102 18-19 1.6459025798147024 1102 20-21 1.5964340718965886 1102 22-23 1.4834726413171921 1102 24-25 1.736990105491266 1102 26-27 1.8390617609711057 1102 28-29 1.580702981652827 1102 30-31 1.7282080922675362 1102 32-33 1.643322280898417 1102 34-35 1.6876989937408098 1102 36-37 1.6447815952909863 1102 38-39 1.6494456890457698 1102 40-41 1.616598260771788 1102 42-43 1.5585848092835057 1102 44-45 1.7195014958182107 1102 46-47 1.6793663554746416 1102 48-49 1.5657232781150938 1102 50-51 1.6887423807883302 1102 52-53 1.7777215933011483 1102 54-55 1.6813462916053723 1102 56-57 1.8852295182341265 1102 58-59 1.7465248864846998 1102 60-61 1.6373685126223272 1102 62-63 1.7420023870596921 1102 64-65 1.626228384239699 1102 66-67 1.5846647066088941 1102 68-69 1.5032488137249267 1102 70-71 1.365371104149645 1102 72-73 1.414526772692689 1102 74-75 1.3426054744889413 1102 76-77 1.323816363987369 1102 78-79 1.1129365630683559 1102 80-81 1.2643914534753407 1102 82-83 1.0271855228432436 1102 84-85 0.9404115210642807 1102 86-87 0.8699423107867226 1102 88-89 0.4959161054088632 1102 90-91 -5.0363154234520735 1102 92-93 -9.409503558994187 1102 94-95 -7.2314321000676784 1102 96-97 -3.988816151680064 1102 98-99 -2.551183839301393 1102 100-101 -1.630563844444719 1103 1 1.959286936699062 1103 2 1.3047443882793175 1103 3 0.9664715265780792 1103 4 0.8759412765764978 1103 5 0.9272860069259252 1103 6 0.7738954238447349 1103 7 0.6732441243665335 1103 8 1.0380030968042462 1103 9 0.8709051863663575 1103 10-11 0.8755168723182933 1103 12-13 0.8718336984325177 1103 14-15 1.0757033973602788 1103 16-17 0.8443304180167885 1103 18-19 0.9209844798966031 1103 20-21 0.8809481314106407 1103 22-23 0.9637893216338753 1103 24-25 0.78284050134166 1103 26-27 0.840433587342929 1103 28-29 0.639773415723262 1103 30-31 1.045618684678125 1103 32-33 0.9652305528066876 1103 34-35 1.050379856421678 1103 36-37 0.9603153108247042 1103 38-39 1.0448661094661915 1103 40-41 0.8233277175012361 1103 42-43 0.9909626416613442 1103 44-45 0.8200884464051619 1103 46-47 0.6028307289390114 1103 48-49 0.6962378836297027 1103 50-51 0.631453273499222 1103 52-53 0.6675386831182415 1103 54-55 0.5832095184685997 1103 56-57 0.5098200178246302 1103 58-59 0.7268347417945549 1103 60-61 0.8148732901270996 1103 62-63 0.34813806819537163 1103 64-65 0.5467648297761478 1103 66-67 0.4213491182933069 1103 68-69 0.38904597452208733 1103 70-71 0.3872725510510904 1103 72-73 0.6958945040604192 1103 74-75 0.49750582938929355 1103 76-77 0.29838638855739674 1103 78-79 -0.015762590630796325 1103 80-81 -0.03134291725902827 1103 82-83 -0.4274486567909399 1103 84-85 -0.4421710615183052 1103 86-87 -0.4417148008703897 1103 88-89 -1.2759921664994103 1103 90-91 -6.267253179389829 1103 92-93 -9.25739463188526 1103 94-95 -8.032135075770654 1103 96-97 -5.383765646629557 1103 98-99 -4.005545018662573 1103 100-101 -3.2197325586134316 1104 1 2.0910530567168237 1104 2 1.1924950632962883 1104 3 0.9906021710725454 1104 4 0.7847019505216082 1104 5 0.7430839560993121 1104 6 0.8377410555023914 1104 7 0.8133008308962033 1104 8 0.8502212867889867 1104 9 0.9156066221491486 1104 10-11 0.9777249051478591 1104 12-13 0.8021598247793591 1104 14-15 0.9511024102277119 1104 16-17 0.748571795919112 1104 18-19 1.0629677282467043 1104 20-21 1.0101202211815163 1104 22-23 0.812504875351955 1104 24-25 0.8140091866791153 1104 26-27 0.9182912442556699 1104 28-29 0.9264674148351446 1104 30-31 1.090330711464432 1104 32-33 0.9030649350321838 1104 34-35 0.982576379510057 1104 36-37 1.073466912745893 1104 38-39 1.0245027997648393 1104 40-41 0.9565510575209402 1104 42-43 0.9183595261198754 1104 44-45 0.9012894151629744 1104 46-47 0.9537276465741833 1104 48-49 0.7505855543745419 1104 50-51 0.7104248998179941 1104 52-53 0.7185449560547816 1104 54-55 0.7142907961264306 1104 56-57 0.9082401718663071 1104 58-59 0.9250345264016175 1104 60-61 0.9075323210630408 1104 62-63 0.8056417303857444 1104 64-65 0.7152869636974692 1104 66-67 0.7029355740586425 1104 68-69 0.6756137748089373 1104 70-71 0.48557007913639083 1104 72-73 0.419841018216637 1104 74-75 0.29924773046166564 1104 76-77 0.41454698389939537 1104 78-79 0.22673680963767495 1104 80-81 0.09178221991360758 1104 82-83 0.03338964027777891 1104 84-85 0.09428968031130935 1104 86-87 0.03861183588626105 1104 88-89 -0.26152970458874236 1104 90-91 -3.345220319206387 1104 92-93 -7.673172419871234 1104 94-95 -5.5400160584116165 1104 96-97 -3.3947144474217144 1104 98-99 -2.530616220830794 1104 100-101 -1.7512127215362412 1105 1 1.9960334049401922 1105 2 1.364901115695833 1105 3 1.1514278513920573 1105 4 1.0221974324411889 1105 5 0.9981386615864096 1105 6 1.0823464824737314 1105 7 1.0609270029746796 1105 8 0.8741007279196751 1105 9 0.8723628232332459 1105 10-11 0.8825102940803973 1105 12-13 1.0260026117651293 1105 14-15 0.9837141094635129 1105 16-17 1.1486862539562637 1105 18-19 1.0295523651548848 1105 20-21 0.9584572079410734 1105 22-23 0.9950916689504581 1105 24-25 1.1145436538633149 1105 26-27 0.9505220161146326 1105 28-29 0.8467857094793274 1105 30-31 0.9243966781358353 1105 32-33 0.9779394927398357 1105 34-35 0.9763432267800596 1105 36-37 1.0860827719124515 1105 38-39 0.799965142501172 1105 40-41 0.6862002147153774 1105 42-43 1.0257311452020907 1105 44-45 0.9925883565385227 1105 46-47 1.0992423540872949 1105 48-49 0.898721797501512 1105 50-51 0.9204482050208469 1105 52-53 0.9743451822734954 1105 54-55 0.8996362412120469 1105 56-57 0.9604158336339665 1105 58-59 0.6822249427363545 1105 60-61 0.7949642351646666 1105 62-63 0.6992802731631969 1105 64-65 0.5871320248586436 1105 66-67 0.6635325181279548 1105 68-69 0.6307998118275258 1105 70-71 0.7603790609903456 1105 72-73 0.5256125025506613 1105 74-75 0.4469621362299421 1105 76-77 0.4607556325992377 1105 78-79 0.05924100403471755 1105 80-81 0.19843529187860653 1105 82-83 -0.23515741699080195 1105 84-85 -0.1774571968934069 1105 86-87 0.1689173210606647 1105 88-89 -0.1667772414481874 1105 90-91 -2.9204614183631925 1105 92-93 -7.149937498271543 1105 94-95 -4.945456906174336 1105 96-97 -2.753751986900596 1105 98-99 -2.055709474609948 1105 100-101 -1.4599326017494185 1106 1 1.8711318458622053 1106 2 1.353190710861515 1106 3 0.8787913878848315 1106 4 0.8765889933147974 1106 5 1.0823341041075096 1106 6 0.9794094436614955 1106 7 0.958113678068031 1106 8 0.014581755862046464 1106 9 -0.22553658534359045 1106 10-11 -0.30568188522133966 1106 12-13 0.005833710471854658 1106 14-15 0.8839413188878282 1106 16-17 1.017806838960432 1106 18-19 1.0994256255606274 1106 20-21 1.0968199003515409 1106 22-23 1.2141709688570046 1106 24-25 1.3312398704800046 1106 26-27 1.1889148240244083 1106 28-29 1.0199653830725595 1106 30-31 1.2714831096188277 1106 32-33 1.1766257924498476 1106 34-35 1.1902544666478931 1106 36-37 1.0002200194123674 1106 38-39 1.0077415865252206 1106 40-41 1.0265428244708161 1106 42-43 1.2413602410160287 1106 44-45 1.0756954701587809 1106 46-47 1.198390919642236 1106 48-49 1.0900520683854822 1106 50-51 1.246187625361106 1106 52-53 1.154908808688603 1106 54-55 1.4594158607953212 1106 56-57 1.3331170525340568 1106 58-59 1.3029294192003178 1106 60-61 0.7194687079525544 1106 62-63 0.9191891631737619 1106 64-65 0.8787681165172145 1106 66-67 0.8220630514267846 1106 68-69 0.8196409589311351 1106 70-71 0.5957374142239402 1106 72-73 0.7024056825918628 1106 74-75 0.5993896858321506 1106 76-77 0.46632982771657083 1106 78-79 0.41672046399170526 1106 80-81 0.4821989375223943 1106 82-83 0.34441960870068655 1106 84-85 0.44075744885359214 1106 86-87 0.6071869099204754 1106 88-89 0.3075036979857302 1106 90-91 -2.8314277017288347 1106 92-93 -7.5547255091923 1106 94-95 -5.3516418238232895 1106 96-97 -2.722346328297247 1106 98-99 -1.696250945631908 1106 100-101 -1.1105974049311982 1107 1 2.0107106805359685 1107 2 1.1304973474874878 1107 3 0.9267341159083102 1107 4 1.0717180571796021 1107 5 0.8811823746248493 1107 6 0.7920680089277425 1107 7 0.7581821702734999 1107 8 0.9135372315705581 1107 9 0.9015840037910579 1107 10-11 1.0333031445469842 1107 12-13 0.849665489838344 1107 14-15 0.892927743195223 1107 16-17 0.8341901672458292 1107 18-19 0.7560628689055164 1107 20-21 0.9340259848998258 1107 22-23 0.7908090619991412 1107 24-25 0.7258501222306251 1107 26-27 0.8829215381489774 1107 28-29 0.8025137071198571 1107 30-31 1.007196528934216 1107 32-33 1.1838583296611631 1107 34-35 1.1128025292191168 1107 36-37 0.7983773757149208 1107 38-39 0.9203337659210504 1107 40-41 0.9177869349037806 1107 42-43 1.0079944773860454 1107 44-45 0.821116551957946 1107 46-47 0.745777103101112 1107 48-49 0.745183463745299 1107 50-51 0.8118698709176471 1107 52-53 0.5615907208631477 1107 54-55 0.6645249580564361 1107 56-57 0.7490103673897508 1107 58-59 0.7091441244695673 1107 60-61 0.4533152970334591 1107 62-63 0.2905600023449111 1107 64-65 0.6590730121483155 1107 66-67 0.8052632135419557 1107 68-69 0.5596080855777963 1107 70-71 0.5057636780431345 1107 72-73 0.5788691315048844 1107 74-75 0.589771724168898 1107 76-77 0.6161416375740707 1107 78-79 0.493829214070697 1107 80-81 0.4901775894534346 1107 82-83 0.18020373550496416 1107 84-85 0.3901378595017704 1107 86-87 0.6375139583857923 1107 88-89 0.16007785732198698 1107 90-91 -2.7548660540228127 1107 92-93 -4.083365492225408 1107 94-95 -2.4804601916460456 1107 96-97 -0.8696537784043432 1107 98-99 -0.6160222775401962 1107 100-101 -0.18526770902061074 1108 1 1.5727511240268868 1108 2 0.9585552239083341 1108 3 -0.6062557461491913 1108 4 -0.3165977500534396 1108 5 0.2859751712969043 1108 6 0.47865896838100497 1108 7 0.5206500013178612 1108 8 0.2868208194856052 1108 9 0.5958133590018022 1108 10-11 0.644301307580001 1108 12-13 0.47776230265657205 1108 14-15 0.3586292467633996 1108 16-17 0.5092179294952146 1108 18-19 0.6145967388497695 1108 20-21 0.3475229707127525 1108 22-23 0.12688619950348112 1108 24-25 0.1764584995051095 1108 26-27 0.5385396479489941 1108 28-29 0.193320247679182 1108 30-31 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2311 80-81 1.0879961999688597 2311 82-83 1.4023462022006257 2311 84-85 1.408714987436749 2311 86-87 1.7529421184775664 2311 88-89 2.107172643287438 2311 90-91 2.805205703985635 2311 92-93 3.6049528387011396 2311 94-95 3.1902307035467246 2311 96-97 2.463365292004985 2311 98-99 2.2172126819272613 2311 100-101 2.0844017296866646 2312 1 1.2143322107759253 2312 2 0.5051952982071839 2312 3 -0.20599210507768007 2312 4 -0.5023956922835211 2312 5 -0.20542091731388723 2312 6 -0.3368374878721241 2312 7 0.11158637650992631 2312 8 0.01915799116473238 2312 9 0.05461462670448469 2312 10-11 -0.0459149497867557 2312 12-13 -0.04315604885915292 2312 14-15 0.027188470776998486 2312 16-17 -0.1584191125033243 2312 18-19 -0.6327402348845581 2312 20-21 -0.0729893532233703 2312 22-23 0.32619012929620794 2312 24-25 0.29369424854963455 2312 26-27 0.5876810490129714 2312 28-29 0.4556738755258465 2312 30-31 0.29692368182724493 2312 32-33 0.5357835713400334 2312 34-35 0.34117687372050653 2312 36-37 0.5190537830665463 2312 38-39 0.3826959877414353 2312 40-41 0.4982632457511116 2312 42-43 0.2779141086878987 2312 44-45 0.16324698675427385 2312 46-47 0.24463549330980783 2312 48-49 0.29962886229256114 2312 50-51 0.09958252039594839 2312 52-53 0.15828404455236367 2312 54-55 0.1894148917247236 2312 56-57 0.19324868565256992 2312 58-59 0.15129425144308328 2312 60-61 0.38050710104832675 2312 62-63 0.45408712374307925 2312 64-65 0.43995998694074956 2312 66-67 0.49059831664659725 2312 68-69 0.22010043418866232 2312 70-71 0.40825110929013775 2312 72-73 0.5078144544707897 2312 74-75 0.5926450779076191 2312 76-77 0.5701155915139395 2312 78-79 0.762543646259072 2312 80-81 0.7221877284000477 2312 82-83 0.9650684354154357 2312 84-85 1.1542217898331195 2312 86-87 1.5718814150432756 2312 88-89 1.677627405711565 2312 90-91 2.2630153264871673 2312 92-93 3.2763247473027857 2312 94-95 2.9757744179234074 2312 96-97 2.204159696914635 2312 98-99 2.1637043104650573 2312 100-101 2.045071750662654 2313 1 -7.100743005767509 2313 2 -2.7121446260230435 2313 3 -1.7389275234148656 2313 4 -0.845964276627555 2313 5 -0.8632909995589984 2313 6 -0.36864273581706186 2313 7 -0.13270805129594265 2313 8 -0.08468158759797717 2313 9 -0.12919070268452515 2313 10-11 -0.33572174248990194 2313 12-13 -0.5472433890486101 2313 14-15 -0.5582912307626486 2313 16-17 -0.6604790580161719 2313 18-19 -0.7389804043310875 2313 20-21 -0.36468891677426285 2313 22-23 -1.3998527901199793 2313 24-25 -0.5280270986102664 2313 26-27 -0.6185554233015367 2313 28-29 -0.76386396689756 2313 30-31 -0.33265356184296024 2313 32-33 -0.4787480328428728 2313 34-35 -0.5893536138213662 2313 36-37 -0.3831560034562287 2313 38-39 -0.30668718864685474 2313 40-41 -0.18701758759316078 2313 42-43 -0.1952937400554653 2313 44-45 -0.48552681118888685 2313 46-47 -1.3674356963067176 2313 48-49 -1.0549054691949777 2313 50-51 -1.1070941249550543 2313 52-53 -1.3227631975612901 2313 54-55 -1.15488521573322 2313 56-57 -0.8255375673389587 2313 58-59 -0.6941913494332965 2313 60-61 -0.5407943537195372 2313 62-63 -0.6196383770642626 2313 64-65 -0.6631424426375965 2313 66-67 -0.43576365454631727 2313 68-69 -0.38072598198034413 2313 70-71 -0.24182833886200683 2313 72-73 -0.28422079795089417 2313 74-75 -0.33621406484534333 2313 76-77 -0.2200070648593382 2313 78-79 -0.10624540637227753 2313 80-81 -0.16313889044144858 2313 82-83 0.29394426884409697 2313 84-85 0.4159887831780118 2313 86-87 0.4202207731836545 2313 88-89 0.9778603786102487 2313 90-91 1.8005390731929047 2313 92-93 2.7269616050834937 2313 94-95 2.3889545060276696 2313 96-97 1.64500786186459 2313 98-99 1.1975342740054433 2313 100-101 0.8565403881328724 2314 1 0.5462331379575289 2314 2 -0.09287492755561289 2314 3 -1.2479783358271739 2314 4 -0.224204632863092 2314 5 -0.18263342102324742 2314 6 -3.5463387822630743 2314 7 -2.8447030049188875 2314 8 -2.7252255786920863 2314 9 -3.569338192724608 2314 10-11 -2.80257096301861 2314 12-13 -1.4079357163740767 2314 14-15 -1.3062824193839049 2314 16-17 -2.3025694087157476 2314 18-19 -3.3862219444101918 2314 20-21 -2.7567248007790184 2314 22-23 -4.320605878374707 2314 24-25 -3.300943702888638 2314 26-27 -2.089577355865039 2314 28-29 -1.8665249637163797 2314 30-31 -1.8935449913814608 2314 32-33 -2.149479210787831 2314 34-35 -1.6027050285405338 2314 36-37 -1.885451572023964 2314 38-39 -3.202612393030897 2314 40-41 -2.6658108803734173 2314 42-43 -1.7361281011357228 2314 44-45 -2.2531555442180142 2314 46-47 -1.3285420804077717 2314 48-49 -2.1127148942821847 2314 50-51 -2.9709735437974842 2314 52-53 -1.9650597386995123 2314 54-55 -2.455688355652324 2314 56-57 -1.2499101838200737 2314 58-59 -0.688712123566436 2314 60-61 -1.1648900898221513 2314 62-63 -0.6615403677841805 2314 64-65 -1.1608748972873748 2314 66-67 -0.6529043028002626 2314 68-69 -0.6768517172120347 2314 70-71 -0.8787984081425435 2314 72-73 -1.680927218207394 2314 74-75 -2.064745601542434 2314 76-77 -1.0165172951195203 2314 78-79 -1.7048691367076039 2314 80-81 -0.727587894916649 2314 82-83 -0.1476281468589029 2314 84-85 -1.4742404275096028 2314 86-87 -0.27525586740402375 2314 88-89 1.0026894987993558 2314 90-91 2.010332555816724 2314 92-93 3.0787968098823892 2314 94-95 2.589289740635575 2314 96-97 1.6867257785087073 2314 98-99 1.7707464408073257 2314 100-101 1.8089310021133258 2315 1 -2.9883504541586667 2315 2 -2.1623160465777254 2315 3 -2.902198221752684 2315 4 -4.429229070966734 2315 5 -4.386007949588372 2315 6 -3.1268921125360194 2315 7 -5.855475272996934 2315 8 -3.267218611807291 2315 9 -2.74464865715359 2315 10-11 -4.172993656700704 2315 12-13 -3.29187542671729 2315 14-15 -2.0793385475121724 2315 16-17 -2.5013338518000197 2315 18-19 -2.0887227620140294 2315 20-21 -1.4802785421041662 2315 22-23 -0.9126530683678311 2315 24-25 -2.263264617644815 2315 26-27 -4.0943892169714005 2315 28-29 -3.3804756854580447 2315 30-31 -3.4038891050839055 2315 32-33 -4.131610741492228 2315 34-35 -3.85326249933933 2315 36-37 -3.227721840348039 2315 38-39 -2.1855225226173545 2315 40-41 -1.7166226334660593 2315 42-43 -3.1031779164622684 2315 44-45 -6.648380137741391 2315 46-47 -3.480078875580759 2315 48-49 -2.519101955438952 2315 50-51 -3.403395211349263 2315 52-53 -3.37331048654449 2315 54-55 -2.1692943336115214 2315 56-57 -1.7642298024116343 2315 58-59 -2.973533345861668 2315 60-61 -3.055654016840883 2315 62-63 -4.840592457399559 2315 64-65 -4.185374358718974 2315 66-67 -3.632879609664233 2315 68-69 -2.2803257084089203 2315 70-71 -3.8392982416213925 2315 72-73 -2.812053621260585 2315 74-75 -1.7341796696690892 2315 76-77 -3.4797594768766054 2315 78-79 -2.0034316541473203 2315 80-81 -1.4979667830354408 2315 82-83 -1.2953651094531544 2315 84-85 -2.546284227072146 2315 86-87 -1.958693914035944 2315 88-89 -1.4398596549431701 2315 90-91 -0.5931648383523367 2315 92-93 0.11430541947580686 2315 94-95 0.16319962719116887 2315 96-97 -0.2689137377098536 2315 98-99 -0.538788470126363 2315 100-101 -1.0264223377608381 2316 1 -7.705690866813775 2316 2 -6.2805351587836675 2316 3 -3.6734242464387137 2316 4 -4.010187527386705 2316 5 -2.0585693620504664 2316 6 -2.7966443689880514 2316 7 -3.23435736826681 2316 8 -2.447179943792804 2316 9 -7.209333506292719 2316 10-11 -6.787636958988401 2316 12-13 -5.65903067781403 2316 14-15 -4.955908903997248 2316 16-17 -4.8036702896972905 2316 18-19 -4.290557693429008 2316 20-21 -2.8667148036726786 2316 22-23 -3.5458548911950487 2316 24-25 -3.1738731318050952 2316 26-27 -4.131299668785232 2316 28-29 -4.506168487384247 2316 30-31 -6.824253605098626 2316 32-33 -4.952555588864801 2316 34-35 -5.534055259064399 2316 36-37 -4.421250345740951 2316 38-39 -6.6063466551223655 2316 40-41 -3.937706188437126 2316 42-43 -4.6647618002096 2316 44-45 -3.332332257448666 2316 46-47 -4.3687818941067285 2316 48-49 -5.316401246525356 2316 50-51 -4.93828229878412 2316 52-53 -6.1910729105252 2316 54-55 -5.282921537312138 2316 56-57 -4.7332073900116995 2316 58-59 -3.4700062798872295 2316 60-61 -3.5327397832821497 2316 62-63 -4.333022261213365 2316 64-65 -3.117773869316693 2316 66-67 -3.934946320167736 2316 68-69 -3.066499930386879 2316 70-71 -2.670460833752358 2316 72-73 -2.3361794198924812 2316 74-75 -2.484979855484923 2316 76-77 -3.155232603915099 2316 78-79 -5.133714401735478 2316 80-81 -5.898652960365251 2316 82-83 -3.664133981374352 2316 84-85 -1.9804342668197243 2316 86-87 -1.9496460239290023 2316 88-89 -1.1595088621180167 2316 90-91 -1.1442846404977267 2316 92-93 -1.371774095309311 2316 94-95 -1.740823874586841 2316 96-97 -3.2276696548647763 2316 98-99 -4.42408873609164 2316 100-101 -3.646604784052535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15943.0 3 5491.0 4 5567.0 5 6283.0 6 7158.0 7 8523.0 8 10247.0 9 12736.0 10 14913.0 11 17694.0 12 20518.0 13 23169.0 14 25536.0 15 28306.0 16 31824.0 17 33514.0 18 30102.0 19 28412.0 20 29852.0 21 31748.0 22 35016.0 23 38906.0 24 43445.0 25 48341.0 26 54070.0 27 60648.0 28 67807.0 29 76840.0 30 85746.0 31 95567.0 32 105520.0 33 116038.0 34 130843.0 35 144479.0 36 143354.0 37 98507.0 38 24213.0 39 313.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.427877876166704 18.791069780163426 13.758476073256066 22.022576270413804 2 16.30009207095845 19.759134168353114 39.541400025524624 24.399373735163813 3 19.646976842505907 24.814978784309904 29.231842166889166 26.30620220629502 4 13.071484619185005 16.21175452922449 36.084782956793084 34.63197789479743 5 14.026963951582944 37.39986234277106 32.76618099190694 15.806992713739056 6 32.31641991771236 35.54507014964498 16.956041075437938 15.182468857204729 7 28.98021095545584 33.605521049943455 19.421605377403232 17.992662617197475 8 28.063068993680908 32.88643124085578 19.103767804696776 19.946731960766545 9 28.19227949852423 15.983827554718522 19.124891269775222 36.69900167698202 10-11 25.790303752966736 26.030720703672717 25.97275388554375 22.206221657816798 12-13 27.32494703352098 23.644179243979206 26.66130946529146 22.369564257208356 14-15 23.854041872653966 24.620958668792937 25.603570813845995 25.9214286447071 16-17 22.677569546178468 27.91464476972103 26.28183445811072 23.12595122598978 18-19 22.79172633933979 25.3885100713222 29.61474609722905 22.205017492108958 20-21 23.608458864558525 24.568169925177198 29.811666109932407 22.011705100331874 22-23 23.18569638460782 24.63558825500055 29.944274405429717 22.234440954961915 24-25 22.766908793596468 25.0660261914587 29.906635072576766 22.260429942368066 26-27 22.205647801870583 25.365137966362354 29.73501399857162 22.69420023319544 28-29 22.2855024206601 25.816029832229358 29.629934902424935 22.2685328446856 30-31 22.38084007607345 25.37803594410672 29.427358780315238 22.813765199504594 32-33 22.040952398063173 25.49351087490614 29.357270189949748 23.108266537080937 34-35 22.99084330973064 25.584554529527704 29.203175186144975 22.221426974596685 36-37 23.214973696960815 25.022635699413883 29.101025514817792 22.661365088807514 38-39 22.63878719031358 25.336888411380816 28.98600437512974 23.03832002317587 40-41 22.873982158764413 25.431146084383727 29.224889447528497 22.469982309323363 42-43 22.696866350801166 25.451522572635803 28.786330020994022 23.065281055569002 44-45 22.53135786080571 25.648070059593763 29.06831072693443 22.752261352666096 46-47 23.11217277960927 26.00602421147874 28.16077909257 22.72102391634199 48-49 22.81388345218952 26.265091654621298 27.70785426431687 23.213170628872312 50-51 22.957379041402852 26.550570756553753 27.64432944841368 22.847720753629723 52-53 23.246051581692313 26.085050141339437 27.6779680212564 22.990930255711845 54-55 22.49100341036002 26.48470472786297 27.90550264655919 23.118789215217824 56-57 22.72908239277395 26.50004677129748 27.56232560580238 23.20854523012619 58-59 22.661879223533003 25.959311486583797 27.615371342931986 23.763437946951214 60-61 22.15575161896768 26.307504928036696 28.059702088278716 23.47704136471691 62-63 22.356286088483103 26.283391557654053 28.009366826904706 23.350955526958135 64-65 22.05905998481051 26.364345973758212 27.834488130073144 23.742105911358134 66-67 22.07168665819126 26.459063871474136 27.7809821112658 23.688267359068803 68-69 22.777122974362307 25.982818110436924 27.805015318978597 23.435043596222176 70-71 22.434286835241213 26.16508497347751 27.356800786493658 24.04382740478762 72-73 22.383219886738797 26.448130448609618 27.259327078440016 23.909322586211566 74-75 22.519150921842677 26.777291400632127 27.127037989804236 23.57651968772096 76-77 22.63456122168658 26.287673847201887 27.20361927958444 23.874145651527094 78-79 22.73113540574656 26.602796973392383 27.084572431056248 23.58149518980481 80-81 22.95465484082733 26.587311663342454 26.782370681747956 23.67566281408225 82-83 22.88626493732358 26.604997338199322 26.8192537486313 23.6894839758458 84-85 22.770950523752397 26.78652818588233 26.74271815030379 23.699803140061483 86-87 22.922102092594415 26.600293502303952 26.589825538693674 23.887778866407963 88-89 22.755770842377835 26.610424854256387 26.429981738905717 24.203822564460065 90-91 22.69143268703128 26.52343035485641 26.72878178159868 24.056355176513627 92-93 23.047228419770647 25.95028572328133 27.075604684344828 23.926881172603196 94-95 22.67895739170904 25.87328009319404 27.118808121357823 24.328954393739096 96-97 23.261138529106955 25.475319238302202 26.63214434091044 24.6313978916804 98-99 22.99623721393421 25.888641740694574 26.19216496775672 24.92295607761449 100-101 23.3736559063044 25.669456150557906 26.34514995783515 24.61173798530254 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2751.0 1 1713.5 2 677.5 3 719.0 4 698.5 5 646.0 6 836.0 7 1539.0 8 2504.0 9 2932.0 10 2673.5 11 2331.0 12 2261.5 13 2383.5 14 2532.5 15 2661.5 16 2813.5 17 3084.0 18 3426.0 19 3846.5 20 4391.5 21 4945.0 22 5759.5 23 6821.0 24 7864.5 25 9313.0 26 11140.0 27 13044.0 28 14984.0 29 16932.5 30 19016.0 31 22375.5 32 26977.0 33 28165.5 34 27114.5 35 27983.5 36 29852.0 37 32327.0 38 35019.5 39 37009.5 40 38932.0 41 41728.0 42 44148.0 43 46073.5 44 49037.5 45 53485.0 46 57032.5 47 59920.5 48 62757.5 49 63789.0 50 64077.0 51 64334.5 52 65234.0 53 67963.0 54 69252.0 55 66589.0 56 62767.0 57 58639.5 58 54621.5 59 48798.0 60 40919.5 61 34308.5 62 28949.5 63 22657.5 64 16610.0 65 12607.0 66 9461.5 67 7210.5 68 5466.0 69 4011.0 70 3001.5 71 2276.0 72 1825.5 73 1532.0 74 1263.0 75 980.5 76 679.5 77 457.0 78 312.0 79 227.0 80 163.0 81 119.5 82 89.5 83 59.5 84 33.0 85 20.5 86 21.5 87 16.5 88 8.5 89 8.0 90 4.5 91 1.5 92 4.0 93 4.0 94 3.5 95 3.0 96 1.5 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1279885089196438 2 0.1150132398962206 3 0.12064723828797015 4 0.13134614432482788 5 0.1197936021680081 6 0.13481759787934025 7 0.16418268040603484 8 0.15171959305458887 9 0.16202013556879766 10-11 0.15576013735574262 12-13 0.14463441325890386 14-15 0.1497277754413441 16-17 0.1448620495575604 18-19 0.16085349953818287 20-21 0.1656623163473024 22-23 0.19955167030979593 24-25 0.22237220925011483 26-27 0.23634338708016042 28-29 0.23056711600175053 30-31 0.22100639145817552 32-33 0.2224860273994431 34-35 0.21153103052659675 36-37 0.2204942097861983 38-39 0.20720594085212235 40-41 0.21187248497458155 42-43 0.20455966888024 44-45 0.1981573979805246 46-47 0.21423421157314324 48-49 0.25270474604609977 50-51 0.254468927360688 52-53 0.255720927003299 54-55 0.2527616551207639 56-57 0.22632738993927232 58-59 0.22715257152190232 60-61 0.19622248944194393 62-63 0.19007630937821715 64-65 0.19036085475153783 66-67 0.19847039789117732 68-69 0.1981573979805246 70-71 0.2039052145216024 72-73 0.2368271142148056 74-75 0.23933111350002761 76-77 0.25407056383803905 78-79 0.2504852921341984 80-81 0.26420037912825545 82-83 0.26269228864965577 84-85 0.2370832050507942 86-87 0.2402416586946538 88-89 0.2436846577118341 90-91 0.4409884195723966 92-93 0.5865333780259266 94-95 0.3416820842834778 96-97 0.2784561023316217 98-99 0.30893091181426696 100-101 0.32608899782550427 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1757189.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.020138138129326 #Duplication Level Percentage of deduplicated Percentage of total 1 77.16288717896894 41.6834982454479 2 11.679336866048631 12.61838781731387 3 4.437868451200757 7.192028003181326 4 2.210403845799623 4.776252843645918 5 1.20749239896115 3.261445309631124 6 0.761489079898243 2.4681447172068047 7 0.5077221098959634 1.9199052958653695 8 0.3817253210064841 1.6496683657273625 9 0.2660466190552381 1.2934687601291608 >10 1.2728364223173076 11.937137623169566 >50 0.06765480833684684 2.5168661446567415 >100 0.037710492219258464 3.8574886389280136 >500 0.0040091592505751205 1.5503282063337682 >1k 0.002600535730102781 2.3139026023023557 >5k 2.167113108418984E-4 0.9614774264607143 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9447 0.537620028352101 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6979 0.39716843208101127 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4714 0.2682693779667412 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2229 0.12685032742636107 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2120 0.12064723828797015 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2001 0.11387505840293786 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1929 0.10977760502712001 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1850 0.1052817881286532 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1785 0.1015826982754843 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1381814932827373E-4 2 0.0 0.0 0.0 0.0 1.1381814932827373E-4 3 0.0 0.0 0.0 0.0 1.1381814932827373E-4 4 0.0 0.0 0.0 0.0 1.1381814932827373E-4 5 0.0 0.0 0.0 0.0 1.707272239924106E-4 6 0.0 0.0 0.0 0.0 2.8454537332068435E-4 7 0.0 0.0 0.0 0.0 2.8454537332068435E-4 8 0.0 0.0 0.0 0.0 3.414544479848212E-4 9 0.0 0.0 0.0 0.0 3.414544479848212E-4 10-11 0.0 0.0 0.0 0.0 3.414544479848212E-4 12-13 0.0 0.0 0.0 0.0 0.0011666360306148058 14-15 0.0 0.0 0.0 0.0 0.0017357267772561743 16-17 0.0 0.0 0.0 0.0 0.0021909993745692696 18-19 0.0 0.0 0.0 5.6909074664136866E-5 0.00236172659856168 20-21 0.0 0.0 0.0 1.1381814932827373E-4 0.002760090121210638 22-23 0.0 0.0 0.0 4.837271346451634E-4 0.00290236280787098 24-25 0.0 0.0 0.0 0.0013089087172751478 0.0029592718825351173 26-27 0.0 0.0 0.0 0.0031015445691954593 0.0031015445691954593 28-29 0.0 0.0 0.0 0.010101360752884293 0.0032438172558558014 30-31 0.0 0.0 0.0 0.03209671811057319 0.0032438172558558014 32-33 0.0 0.0 0.0 0.07287207010742726 0.003414544479848212 34-35 0.0 0.0 0.0 0.13128923525016375 0.0038129080024971703 36-37 0.0 0.0 0.0 0.22797775310453228 0.0038129080024971703 38-39 0.0 0.0 0.0 0.38382325407227114 0.003869817077161307 40-41 0.0 0.0 0.0 0.5631152938016344 0.003869817077161307 42-43 0.0 0.0 0.0 0.7333018815847356 0.003926726151825444 44-45 0.0 0.0 0.0 0.9386867320476056 0.004211271525146129 46-47 0.0 0.0 0.0 1.1793267542649084 0.004609635047795086 48-49 0.0 0.0 0.0 1.43701104434412 0.004865725883783702 50-51 0.0 0.0 0.0 1.7332227779709526 0.005178725794436455 52-53 0.0 0.0 0.0 2.0021181557589993 0.005377907555760934 54-55 0.0 0.0 0.0 2.2787247131640367 0.0055486347797533446 56-57 0.0 0.0 0.0 2.5899035334275364 0.005719362003745756 58-59 0.0 0.0 0.0 2.9293946183364454 0.005918543765070235 60-61 0.0 0.0 0.0 3.310059418764857 0.006060816451730577 62-63 0.0 0.0 0.0 3.669980861478191 0.006487634511711603 64-65 0.0 0.0 0.0 4.020683034095934 0.006487634511711603 66-67 0.0 0.0 0.0 4.427526008869849 0.006487634511711603 68-69 0.0 0.0 0.0 4.870335518831498 0.0065445435863757395 70-71 0.0 0.0 0.0 5.343249929290475 0.006601452661039877 72-73 0.0 0.0 0.0 5.825042155397057 0.00708517979568504 74-75 2.8454537332068433E-5 0.0 0.0 6.282733388383378 0.00745508878100193 76-77 5.6909074664136866E-5 0.0 0.0 6.7450627109548265 0.007654270542326409 78-79 5.6909074664136866E-5 0.0 0.0 7.254085929288198 0.007796543228986751 80-81 5.6909074664136866E-5 0.0 0.0 7.7861573228605465 0.007910361378315025 82-83 5.6909074664136866E-5 0.0 0.0 8.306050174454768 0.008137997676971572 84-85 5.6909074664136866E-5 0.0 0.0 8.793163399042449 0.008251815826299845 86-87 5.6909074664136866E-5 0.0 0.0 9.273532898282427 0.008450997587624326 88-89 5.6909074664136866E-5 0.0 0.0 9.775812391268099 0.008593270274284668 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4200 0.0 60.98974 1 GTATCAA 8600 0.0 46.443607 1 GTGGTAT 1630 0.0 38.123783 1 TATCAAC 11405 0.0 36.31145 2 TGGTATC 1680 0.0 35.57836 2 ATCAACG 11825 0.0 35.143097 3 TCAACGC 11950 0.0 34.77648 4 CAACGCA 12145 0.0 33.981804 5 AACGCAG 12540 0.0 32.949234 6 ACGCAGA 13825 0.0 29.474926 7 CGCAGAG 14065 0.0 28.906168 8 TACATGG 9750 0.0 26.95441 2 GCAGAGT 14235 0.0 26.59393 9 GTACATG 9650 0.0 26.397285 1 AGGTAAG 915 0.0 24.885412 2 TAAGGTG 1055 0.0 24.730614 5 GTAAGGT 1085 0.0 24.485428 4 ACATGGG 10475 0.0 24.09321 3 GTATAGA 405 0.0 23.425415 1 GAGTACT 8675 0.0 23.130732 12-13 >>END_MODULE