FastQCFastQC Report
Fri 27 May 2016
SRR939893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757189
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105180.5985696473173916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84070.4784345907013986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60470.34412917449403563No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26710.15200413842790958No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26330.14984159359067237No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG25150.1431263227803042No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24220.1378337788365395No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23750.13515905232732506No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23150.13174450784747685No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18670.10624924239794353No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA18530.10545251535264563No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA18410.10476960645667598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40050.062.524471
GTATCAA85300.047.545281
TATCAAC114250.035.3275642
ATCAACG116550.034.6679083
TCAACGC118700.034.0002484
CAACGCA120950.033.3677525
AACGCAG124950.032.3152056
GTGGTAT15900.028.4729251
TGGTATC15450.028.3824982
AGGTAAG11500.027.8809262
ACGCAGA144600.027.8260757
CGCAGAG146450.027.4423948
GGTAAGG13800.026.3069613
TAAGGTG14550.025.5990075
GCAGAGT154300.025.3439649
GTAAGGT14000.025.257614
GTACATG100950.023.5441061
TACATGG100750.022.9322762
GAGTACT92250.022.52343212-13
AAGGTAA13850.022.472541
Produced by FastQC (version 0.11.5)