##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939893_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1757189 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.364903832200177 31.0 31.0 34.0 30.0 34.0 2 31.56876351946205 31.0 31.0 34.0 30.0 34.0 3 31.611643369039985 31.0 31.0 34.0 30.0 34.0 4 35.44320104439534 37.0 35.0 37.0 33.0 37.0 5 35.17332512325083 37.0 35.0 37.0 32.0 37.0 6 35.20960067471399 37.0 35.0 37.0 32.0 37.0 7 35.121676723448644 37.0 35.0 37.0 32.0 37.0 8 35.048549131595976 37.0 35.0 37.0 32.0 37.0 9 36.64092365704543 39.0 35.0 39.0 32.0 39.0 10-11 36.493556470021154 38.0 35.0 39.0 32.0 39.0 12-13 36.4288639412152 38.0 35.0 39.0 32.0 39.0 14-15 37.4092983168003 39.0 36.0 41.0 32.0 41.0 16-17 37.34685910280568 39.0 36.0 41.0 32.0 41.0 18-19 37.36233723293283 39.0 36.0 41.0 32.0 41.0 20-21 37.298675896559786 39.0 36.0 40.5 31.0 41.0 22-23 37.158807902849375 39.0 36.0 40.0 31.0 41.0 24-25 36.97270470051884 39.0 36.0 40.0 31.0 41.0 26-27 36.6445299851069 38.5 35.0 40.0 30.0 41.0 28-29 36.3871430449428 38.0 35.0 40.0 30.0 41.0 30-31 36.060922302609455 38.0 35.0 40.0 29.5 41.0 32-33 35.907709415435676 38.0 34.5 40.0 29.5 41.0 34-35 35.917450541745936 38.0 35.0 40.0 29.5 41.0 36-37 35.817281180339734 38.0 35.0 40.0 29.0 41.0 38-39 35.61884777334709 38.0 34.0 40.0 27.5 41.0 40-41 35.379559341653064 38.0 34.0 40.0 27.0 41.0 42-43 35.09281756259571 38.0 34.0 40.0 26.5 41.0 44-45 34.7416991000968 38.0 33.0 40.0 25.5 41.0 46-47 34.306733083350736 38.0 33.0 40.0 24.0 41.0 48-49 33.872438309140335 37.5 33.0 40.0 23.0 41.0 50-51 33.51168997757213 37.0 32.5 40.0 22.0 41.0 52-53 33.15794402309598 37.0 32.0 40.0 20.0 41.0 54-55 32.78813889684035 36.5 32.0 40.0 18.5 41.0 56-57 31.456635854196673 35.5 29.5 39.0 10.5 40.5 58-59 28.06528011500186 33.5 22.0 38.0 2.0 40.0 60-61 28.03374964218419 33.5 21.5 38.0 2.0 40.0 62-63 29.751717942691425 34.0 27.5 38.0 2.0 40.0 64-65 29.211489771447468 34.0 27.5 37.5 2.0 40.0 66-67 23.852191767647078 27.5 16.0 35.0 2.0 38.0 68-69 16.407351172810664 13.0 7.0 28.5 2.0 34.5 70-71 15.318921015326183 11.5 6.5 24.5 2.0 32.5 72-73 17.275947550320424 15.5 7.0 29.0 2.0 34.0 74-75 17.979658135806677 17.0 7.0 30.0 2.0 34.5 76-77 18.976091075006728 21.0 7.0 30.5 2.0 34.0 78-79 21.964856085486534 27.5 7.5 33.5 2.0 35.0 80-81 24.515653410077118 30.5 15.5 34.0 2.0 36.0 82-83 25.449443969885994 32.0 20.0 35.0 2.0 35.5 84-85 25.445091279310308 32.0 20.0 35.0 2.0 35.0 86-87 25.17355247500411 32.0 18.5 35.0 2.0 35.0 88-89 24.733321515215493 32.0 13.5 34.5 2.0 35.0 90-91 23.155548720143365 31.5 2.0 34.0 2.0 35.0 92-93 21.103718211302255 29.5 2.0 34.0 2.0 35.0 94-95 16.816269906082955 17.0 2.0 32.5 2.0 35.0 96-97 15.533677652204744 6.5 2.0 31.5 2.0 34.0 98-99 14.46439113834653 2.0 2.0 31.0 2.0 34.0 100-101 13.861302625955432 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.30974027423641814 1101 2 0.4707818183870103 1101 3 0.474450724135945 1101 4 0.23629467374318125 1101 5 0.45448384630594774 1101 6 0.4274028190856143 1101 7 0.4102692181063432 1101 8 0.2085738073572685 1101 9 0.4250003082018168 1101 10-11 0.45459699532055 1101 12-13 0.4492387766294712 1101 14-15 0.47451496308109853 1101 16-17 0.35271288131560397 1101 18-19 0.3959163279287665 1101 20-21 0.4729497854914797 1101 22-23 0.3923169824498203 1101 24-25 0.36339225118648244 1101 26-27 0.5055720450459305 1101 28-29 0.40449628939114035 1101 30-31 0.409156952093646 1101 32-33 0.2456641420408161 1101 34-35 0.6011580610115672 1101 36-37 0.5901177438251253 1101 38-39 0.6217728510298812 1101 40-41 0.41586473330669804 1101 42-43 0.6284836404387733 1101 44-45 0.5191769113858484 1101 46-47 0.5509810221960123 1101 48-49 0.5050096494773939 1101 50-51 0.6735124799509364 1101 52-53 0.768485039227059 1101 54-55 0.5298731512500012 1101 56-57 1.1443114569841235 1101 58-59 -7.962098107080717 1101 60-61 -9.094152055472886 1101 62-63 -2.4007914099892353 1101 64-65 0.10912183098855266 1101 66-67 -1.049912821777344 1101 68-69 -3.317919421318649 1101 70-71 -3.887951861374688 1101 72-73 -6.399606917084515 1101 74-75 -7.084086371671496 1101 76-77 -8.215046041807483 1101 78-79 -10.970426397315133 1101 80-81 -7.5404009970345065 1101 82-83 -3.872827489838439 1101 84-85 -3.028172321765094 1101 86-87 -2.886292664077825 1101 88-89 -2.9642492677173458 1101 90-91 -8.157372865172562 1101 92-93 -13.44259298044654 1101 94-95 -11.257690126501679 1101 96-97 -11.31411874296866 1101 98-99 -11.06301506510447 1101 100-101 -10.854621046697432 1102 1 0.48767509901334805 1102 2 0.49923132052072106 1102 3 0.48817372943790005 1102 4 0.36201560999043636 1102 5 0.4991974115458291 1102 6 0.43877615414315585 1102 7 0.503123220434027 1102 8 0.5487858854640777 1102 9 0.708896596834947 1102 10-11 0.6519751150670956 1102 12-13 0.6825234009667227 1102 14-15 0.813940153822081 1102 16-17 0.7248465939756343 1102 18-19 0.8715299319634298 1102 20-21 0.8906951624473862 1102 22-23 0.8915921667249975 1102 24-25 0.8351703450698409 1102 26-27 0.9567960202172756 1102 28-29 0.823605302710682 1102 30-31 0.8346489991646351 1102 32-33 0.6654313733869941 1102 34-35 0.7436771351095857 1102 36-37 0.8648338953833772 1102 38-39 0.8411508414605109 1102 40-41 0.8912261712997207 1102 42-43 1.0089621093382846 1102 44-45 0.9262084644700366 1102 46-47 1.102796617695823 1102 48-49 1.054192372870645 1102 50-51 0.9177773189526164 1102 52-53 0.8953833969148874 1102 54-55 0.7439065147570574 1102 56-57 1.5757041894294836 1102 58-59 -7.7973392812692595 1102 60-61 -8.966391807186323 1102 62-63 -2.092297293863542 1102 64-65 0.6571562290229487 1102 66-67 -0.6658225592660294 1102 68-69 -3.40869919630895 1102 70-71 -3.780385586966309 1102 72-73 -6.278580148689326 1102 74-75 -6.847209997689859 1102 76-77 -7.803319674291643 1102 78-79 -10.858079313389101 1102 80-81 -7.194619282305425 1102 82-83 -3.0329315891530655 1102 84-85 -2.1334619894231857 1102 86-87 -1.8497751417708272 1102 88-89 -2.0171116755797556 1102 90-91 -7.623095741158593 1102 92-93 -13.255134684830349 1102 94-95 -11.469331792090713 1102 96-97 -11.306765193246688 1102 98-99 -10.719708457061019 1102 100-101 -10.514961147563849 1103 1 0.270899232237479 1103 2 0.321685592974994 1103 3 0.28669952796369813 1103 4 0.21138770936253337 1103 5 0.28725364960207145 1103 6 0.24951871488571697 1103 7 0.24093375824456587 1103 8 0.37163600831420496 1103 9 0.4443729823113358 1103 10-11 0.28999040308237767 1103 12-13 0.32033393877726013 1103 14-15 0.49255058243250716 1103 16-17 0.29502813915717496 1103 18-19 0.321495806929299 1103 20-21 0.337077908830139 1103 22-23 0.3868624369952727 1103 24-25 0.37467348457298044 1103 26-27 0.37957789299914424 1103 28-29 0.3914526955580726 1103 30-31 0.4656481801638179 1103 32-33 0.3838725418281612 1103 34-35 0.6069927234251722 1103 36-37 0.28844841899790197 1103 38-39 0.32195892226858547 1103 40-41 0.2896632447367935 1103 42-43 0.3198206951968743 1103 44-45 0.44007005333162397 1103 46-47 0.31218782708702975 1103 48-49 0.33393575261402475 1103 50-51 0.1336932348685309 1103 52-53 0.248700350231843 1103 54-55 0.14377001462055006 1103 56-57 0.7836894474147442 1103 58-59 -6.771356480286457 1103 60-61 -8.346988312782827 1103 62-63 -2.551285827852073 1103 64-65 -0.4060296841629629 1103 66-67 -1.9013535447970114 1103 68-69 -4.193916231525986 1103 70-71 -4.832146438727161 1103 72-73 -7.36774886285804 1103 74-75 -7.716593017072878 1103 76-77 -8.554780225752193 1103 78-79 -11.644238199547988 1103 80-81 -8.32376888645503 1103 82-83 -4.8897838960053726 1103 84-85 -4.020787951749146 1103 86-87 -3.7254712924669775 1103 88-89 -3.9014209848890644 1103 90-91 -8.96895978702264 1103 92-93 -14.166580221275886 1103 94-95 -12.10523132799025 1103 96-97 -11.683321294802791 1103 98-99 -11.188238350590913 1103 100-101 -10.894158526761228 1104 1 0.21816930537920598 1104 2 0.12254639911624565 1104 3 0.199235703258843 1104 4 0.12884806328069942 1104 5 -0.021755083509745532 1104 6 0.14869746550458984 1104 7 0.15117717268201858 1104 8 0.20409041595809896 1104 9 0.1523517084305368 1104 10-11 0.18402307441419197 1104 12-13 0.137791740646378 1104 14-15 0.25829863904716177 1104 16-17 0.14477612431446119 1104 18-19 0.20588122247944085 1104 20-21 0.23255325348435463 1104 22-23 0.2166019748702368 1104 24-25 0.2505487097913104 1104 26-27 0.1592828506096069 1104 28-29 0.19376625561543648 1104 30-31 0.09274940974105306 1104 32-33 0.04193471995098008 1104 34-35 -0.02764968346331642 1104 36-37 -0.018719521141235873 1104 38-39 -0.03278686812652154 1104 40-41 0.2533714485536436 1104 42-43 0.11462842382218952 1104 44-45 -0.08257836108030858 1104 46-47 -0.006845099449684255 1104 48-49 -0.19223882795671443 1104 50-51 -0.26392136489361917 1104 52-53 -0.20461514427870497 1104 54-55 -0.3825589649171235 1104 56-57 0.3859771041743585 1104 58-59 -6.367825082427117 1104 60-61 -8.899041819043003 1104 62-63 -3.173128049972213 1104 64-65 -0.9171035353580876 1104 66-67 -1.983631605852235 1104 68-69 -4.254304091939112 1104 70-71 -4.644040263278892 1104 72-73 -7.373010549966619 1104 74-75 -8.25784956945879 1104 76-77 -9.011112369830673 1104 78-79 -11.76316036715636 1104 80-81 -8.321950423911453 1104 82-83 -4.915189927007663 1104 84-85 -3.989124424102801 1104 86-87 -3.772869350868067 1104 88-89 -3.9367505309816195 1104 90-91 -9.270011358829644 1104 92-93 -14.152825952973865 1104 94-95 -11.700956263040602 1104 96-97 -11.436627984744145 1104 98-99 -10.847053255578126 1104 100-101 -10.439685805613419 1105 1 0.0866510565302292 1105 2 0.17081927858554735 1105 3 0.1723891645430129 1105 4 0.08225833735060206 1105 5 0.056343120826774395 1105 6 0.111070172522588 1105 7 0.20432927929132916 1105 8 0.3156562822277138 1105 9 0.26669226050250217 1105 10-11 0.3480882858243959 1105 12-13 0.30444012519661356 1105 14-15 0.4617847389442389 1105 16-17 0.3160668177581343 1105 18-19 0.4024144237020053 1105 20-21 0.45382635413729844 1105 22-23 0.5158875637072313 1105 24-25 0.4152896102424961 1105 26-27 0.5202164853281275 1105 28-29 0.40235980940112626 1105 30-31 0.3863480615398558 1105 32-33 0.3122146820920122 1105 34-35 0.26419733178636307 1105 36-37 0.1649051571984117 1105 38-39 0.49851631110355754 1105 40-41 0.3335478200270643 1105 42-43 0.3256341680032335 1105 44-45 0.3790164568687757 1105 46-47 0.06387731987972955 1105 48-49 0.18859084445772822 1105 50-51 0.1799493907332277 1105 52-53 0.16670692846973623 1105 54-55 0.2657244342617986 1105 56-57 0.5085519514587453 1105 58-59 -6.587157731230061 1105 60-61 -8.325388866361319 1105 62-63 -2.710522320896395 1105 64-65 -0.41258635406482824 1105 66-67 -1.7166057105829857 1105 68-69 -3.7444662153499912 1105 70-71 -4.41840762712358 1105 72-73 -5.914834487790639 1105 74-75 -6.591228158522966 1105 76-77 -8.272102918875598 1105 78-79 -11.331076331759785 1105 80-81 -7.901450506930242 1105 82-83 -4.278508328946888 1105 84-85 -3.3474999112013215 1105 86-87 -3.1186638946453478 1105 88-89 -3.155660892865626 1105 90-91 -8.26559787609845 1105 92-93 -10.93076117673798 1105 94-95 -10.917957342584591 1105 96-97 -11.328587120317726 1105 98-99 -10.818215164998332 1105 100-101 -10.3742620531281 1106 1 0.16573676128239967 1106 2 0.15056978651949748 1106 3 0.265570478229165 1106 4 0.1234974841064016 1106 5 0.06762229433304867 1106 6 0.06023011975683801 1106 7 0.05276843396482178 1106 8 0.1398340796111981 1106 9 0.23886016642903485 1106 10-11 0.26304063122795895 1106 12-13 0.27039052369677563 1106 14-15 0.16862449196887042 1106 16-17 0.18041628762637885 1106 18-19 0.2440337609630845 1106 20-21 0.2864244734031587 1106 22-23 0.26616680366554846 1106 24-25 0.3712976523532916 1106 26-27 0.4549089749750408 1106 28-29 0.46959195465680637 1106 30-31 0.46568791000093057 1106 32-33 0.4307290689588257 1106 34-35 0.2791550499081197 1106 36-37 0.35079837866609864 1106 38-39 0.32305570655965 1106 40-41 0.2526654358975051 1106 42-43 0.2800098934969242 1106 44-45 0.20556107800023682 1106 46-47 0.1419966422951333 1106 48-49 0.21924715026153763 1106 50-51 0.4155487386549055 1106 52-53 0.39075528576115204 1106 54-55 0.17253923242433444 1106 56-57 0.982161654587788 1106 58-59 -6.294438612695171 1106 60-61 -8.47165723080693 1106 62-63 -2.472906082845391 1106 64-65 -0.09129533230107967 1106 66-67 -1.422593958468891 1106 68-69 -3.8141712248441486 1106 70-71 -4.224184218190448 1106 72-73 -5.593129908113934 1106 74-75 -6.088350219610772 1106 76-77 -7.935922398372316 1106 78-79 -11.070510457286277 1106 80-81 -7.382875747677506 1106 82-83 -3.5474821539122097 1106 84-85 -2.6179508801993236 1106 86-87 -2.40921908023741 1106 88-89 -2.5560609482894563 1106 90-91 -7.510604782869066 1106 92-93 -11.085621648660577 1106 94-95 -11.142938856192414 1106 96-97 -11.308928980720369 1106 98-99 -10.80176545717523 1106 100-101 -10.571299423210826 1107 1 0.12738548415334705 1107 2 0.10431721079856615 1107 3 0.18443372551507764 1107 4 0.07654291775357791 1107 5 -0.00652324179822017 1107 6 0.11480780320954409 1107 7 0.30370775045182796 1107 8 0.13753817439918237 1107 9 -0.04589163830063825 1107 10-11 0.14314858453123946 1107 12-13 0.12070418603964583 1107 14-15 0.16855422027344247 1107 16-17 0.16600804793416302 1107 18-19 0.018858372253468758 1107 20-21 0.2791222092382384 1107 22-23 0.23725299707504632 1107 24-25 0.27171612302329606 1107 26-27 0.25205910364306305 1107 28-29 0.22047371009097105 1107 30-31 0.12609912470983176 1107 32-33 0.15499510198180388 1107 34-35 0.26937384727796143 1107 36-37 0.24540463564333237 1107 38-39 0.14084337927004498 1107 40-41 -0.0013035530398752826 1107 42-43 0.11622763479394393 1107 44-45 0.25336017576251635 1107 46-47 0.15641160046985192 1107 48-49 0.12246178071958269 1107 50-51 0.11749560962154249 1107 52-53 -0.031465568553826984 1107 54-55 -0.1241228075684333 1107 56-57 0.6120535269671272 1107 58-59 -6.41727498895634 1107 60-61 -8.291356584162067 1107 62-63 -2.809440500723383 1107 64-65 -0.6499275291577007 1107 66-67 -1.873192593418512 1107 68-69 -4.186949858471863 1107 70-71 -4.688417559440694 1107 72-73 -6.1776001176361675 1107 74-75 -6.827340805745713 1107 76-77 -8.714478672744974 1107 78-79 -11.736330050414976 1107 80-81 -7.955413024360592 1107 82-83 -4.226117524797868 1107 84-85 -3.434280279044035 1107 86-87 -3.2446405905668065 1107 88-89 -3.2889415285339183 1107 90-91 -8.13853903470975 1107 92-93 -11.476161407913745 1107 94-95 -11.286547690887964 1107 96-97 -11.79827891487924 1107 98-99 -11.067520661820208 1107 100-101 -10.47087172824591 1108 1 -0.021720143336441566 1108 2 -0.03329604155209509 1108 3 -0.03819716625118019 1108 4 -0.04699153765307784 1108 5 -0.18601310889196299 1108 6 -0.09506347060555953 1108 7 -0.026961099423019164 1108 8 0.006346752115852894 1108 9 0.062118620511519396 1108 10-11 0.11090408535969942 1108 12-13 -0.1445650511217309 1108 14-15 -0.050694367062440904 1108 16-17 0.00611055421686757 1108 18-19 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2311 16-17 -0.4357916446749641 2311 18-19 -0.35072251428490375 2311 20-21 -0.37968992397786394 2311 22-23 -0.40712688984678636 2311 24-25 -0.40078033269340096 2311 26-27 -0.4541645260419429 2311 28-29 -0.4099971790895367 2311 30-31 -0.43972428808197606 2311 32-33 -0.4175734318114266 2311 34-35 -0.501080542706795 2311 36-37 -0.4461774483262104 2311 38-39 -0.4649066437936824 2311 40-41 -0.5137433755338847 2311 42-43 -0.4741387239170223 2311 44-45 -0.5690473826138245 2311 46-47 -0.5533704331674159 2311 48-49 -0.5205342824430303 2311 50-51 -0.5889705814616093 2311 52-53 -0.6345292162881435 2311 54-55 -0.72414549474707 2311 56-57 0.7029666686487097 2311 58-59 4.128936095315076 2311 60-61 3.474058145622024 2311 62-63 1.0366241680908779 2311 64-65 1.0732309967969975 2311 66-67 -0.3531943004482727 2311 68-69 -3.2862374873384876 2311 70-71 -0.9990050349884356 2311 72-73 -1.3529133293561824 2311 74-75 0.7908347622653018 2311 76-77 3.5121147799172725 2311 78-79 4.42930204325075 2311 80-81 2.335732668746836 2311 82-83 1.1029636099501694 2311 84-85 0.7303246157382937 2311 86-87 0.7678712472452851 2311 88-89 0.9585931627666895 2311 90-91 2.2794025698103653 2311 92-93 4.1734550438366185 2311 94-95 7.965980533154667 2311 96-97 8.70742551417265 2311 98-99 9.33096943270029 2311 100-101 8.347477712042881 2312 1 -0.25021611596228155 2312 2 -0.3602029454386617 2312 3 -0.3033856845969183 2312 4 -0.2696853202604643 2312 5 -0.26266134707626065 2312 6 -0.3443380296701477 2312 7 -0.4565213730790276 2312 8 -0.38344614412682887 2312 9 -0.45857624062752933 2312 10-11 -0.4222094044177922 2312 12-13 -0.34320800578488075 2312 14-15 -0.6004816299031788 2312 16-17 -0.5638989096844185 2312 18-19 -0.434724736150379 2312 20-21 -0.3264955523481916 2312 22-23 -0.2741045607920256 2312 24-25 -0.3713148817416396 2312 26-27 -0.4467973299711048 2312 28-29 -0.6889987636220241 2312 30-31 -0.616623527069045 2312 32-33 -0.3800075851798752 2312 34-35 -0.4797026874416517 2312 36-37 -0.3901545448717627 2312 38-39 -0.5418702027464519 2312 40-41 -0.46991819890731534 2312 42-43 -0.3258032030293023 2312 44-45 -0.5012760854251823 2312 46-47 -0.34391881154161297 2312 48-49 -0.2648085521634229 2312 50-51 -0.2744296825500925 2312 52-53 -0.2101069093133816 2312 54-55 -0.350868760494663 2312 56-57 0.6705026859628376 2312 58-59 4.231410573995355 2312 60-61 3.8554039422376007 2312 62-63 1.416781432007003 2312 64-65 1.1688836124489335 2312 66-67 0.008752173180273104 2312 68-69 -0.7689401632303934 2312 70-71 0.1579332813111307 2312 72-73 6.518718739106713 2312 74-75 5.242915454488934 2312 76-77 5.515398778165807 2312 78-79 5.450789498457443 2312 80-81 3.0109479086320405 2312 82-83 1.7196680810411138 2312 84-85 1.3976190598479228 2312 86-87 1.224949670136791 2312 88-89 1.4145078529455262 2312 90-91 2.8196098816997974 2312 92-93 4.7418947406687835 2312 94-95 8.378433779846272 2312 96-97 9.015071750094657 2312 98-99 9.327587567837284 2312 100-101 8.552850402510778 2313 1 -0.3465703419315247 2313 2 -0.30197391989817746 2313 3 -0.37717668893890277 2313 4 -0.44472201697481495 2313 5 -0.3639772313753227 2313 6 -0.43992094817008365 2313 7 -0.4665309561523756 2313 8 -0.5372714835731145 2313 9 -0.5694784131748278 2313 10-11 -0.5661084802151564 2313 12-13 -0.5450553394159527 2313 14-15 -0.5617567171541467 2313 16-17 -0.6394729765001657 2313 18-19 -0.5714991974340435 2313 20-21 -0.6536886548929743 2313 22-23 -0.6506111086003656 2313 24-25 -0.5524955153068305 2313 26-27 -0.7201648787431125 2313 28-29 -1.0024473760321158 2313 30-31 -0.964995034183481 2313 32-33 -0.7217470268539969 2313 34-35 -0.851100495363859 2313 36-37 -1.0297676266107985 2313 38-39 -1.2895104010734784 2313 40-41 -1.4418984926578418 2313 42-43 -1.2503028109457688 2313 44-45 -1.1243877685498944 2313 46-47 -1.064019930781356 2313 48-49 -1.2036101670063886 2313 50-51 -0.7547324080061486 2313 52-53 -0.817639065654916 2313 54-55 -0.8735281611359795 2313 56-57 0.13965520953674115 2313 58-59 3.4943785994294814 2313 60-61 3.2320842284599145 2313 62-63 0.720876300414556 2313 64-65 0.40617304671539856 2313 66-67 1.2155997439100297 2313 68-69 0.1375084655220924 2313 70-71 1.994971873207497 2313 72-73 7.319573794653444 2313 74-75 8.73509052815697 2313 76-77 7.921514798990838 2313 78-79 5.799472314804017 2313 80-81 2.9303131914371896 2313 82-83 1.4627297296458615 2313 84-85 1.0858440694457308 2313 86-87 1.1280540018627683 2313 88-89 0.9753989474921738 2313 90-91 2.339519339472524 2313 92-93 4.3141884065439555 2313 94-95 7.73689832341255 2313 96-97 8.636743293135568 2313 98-99 9.046189607207452 2313 100-101 8.014983331552902 2314 1 -0.35798815464247014 2314 2 -0.3540218264127226 2314 3 -0.28410141830193325 2314 4 -0.28970527035497895 2314 5 -0.38626915143002805 2314 6 -0.2183248830694069 2314 7 -0.4738529833079781 2314 8 -0.3949888166749318 2314 9 -0.5987920723778828 2314 10-11 -0.3681648349055706 2314 12-13 -0.4488365231441236 2314 14-15 -0.4810053432609678 2314 16-17 -0.5147581196067748 2314 18-19 -0.6016175625595324 2314 20-21 -0.5053896504589872 2314 22-23 -0.5795258215862944 2314 24-25 -0.5195719436873105 2314 26-27 -0.44862031317303774 2314 28-29 -0.7123614683861987 2314 30-31 -0.6189331616665967 2314 32-33 -0.7589368586020484 2314 34-35 -0.7277711393126722 2314 36-37 -0.5545570472417651 2314 38-39 -0.5832806749784467 2314 40-41 -0.7106989808113084 2314 42-43 -0.5645786621578708 2314 44-45 -0.6051543628965064 2314 46-47 -0.5930260214353638 2314 48-49 -0.6031598448904916 2314 50-51 -0.36938239664576145 2314 52-53 -0.6027612922149359 2314 54-55 -0.5546845254696748 2314 56-57 0.8465919723990574 2314 58-59 3.8880156531563053 2314 60-61 3.7109791740880063 2314 62-63 1.4268349980382702 2314 64-65 1.2698759467612533 2314 66-67 1.552526734362079 2314 68-69 -0.2999889073012625 2314 70-71 2.1824184882912974 2314 72-73 8.210557434314428 2314 74-75 9.741064144022946 2314 76-77 8.84012823216742 2314 78-79 6.510219548404184 2314 80-81 3.651577648463995 2314 82-83 2.2150924028300345 2314 84-85 1.7796505812172647 2314 86-87 1.816656437300157 2314 88-89 1.7937645400362392 2314 90-91 2.989211812134087 2314 92-93 5.005119516408982 2314 94-95 8.721032237027963 2314 96-97 9.226125268547117 2314 98-99 9.693819213901595 2314 100-101 8.802308265430472 2315 1 -0.7268580011129728 2315 2 -0.5914542803343714 2315 3 -0.5382105901667593 2315 4 -0.296889982813461 2315 5 -0.6059324486687743 2315 6 -0.6218389293532809 2315 7 -0.7601619445460557 2315 8 -1.0070491483370532 2315 9 -1.134791544310822 2315 10-11 -0.9585757670939543 2315 12-13 -0.7326107624725182 2315 14-15 -0.8550568478020395 2315 16-17 -1.0116017187438615 2315 18-19 -0.876676595056658 2315 20-21 -1.015126337018181 2315 22-23 -0.8069485542733474 2315 24-25 -0.8917192563960299 2315 26-27 -0.895516268959426 2315 28-29 -0.6907549743614609 2315 30-31 -0.824464814186463 2315 32-33 -0.9553587541827895 2315 34-35 -0.9839891289973579 2315 36-37 -0.9688534128802004 2315 38-39 -1.0242574790325634 2315 40-41 -1.0366122217929075 2315 42-43 -0.9967646497782994 2315 44-45 -0.8847630231963635 2315 46-47 -0.9066788053389203 2315 48-49 -0.6860608233147545 2315 50-51 -0.6175223104148131 2315 52-53 -0.44954053317853493 2315 54-55 -0.5025919097074762 2315 56-57 0.8566992060177263 2315 58-59 4.209216195625199 2315 60-61 3.684979672151261 2315 62-63 1.4809689230467455 2315 64-65 1.2156190061297956 2315 66-67 1.8125225038247983 2315 68-69 5.94812280288593 2315 70-71 4.714489411298519 2315 72-73 8.781853088862558 2315 74-75 9.660632629220565 2315 76-77 8.473143075052466 2315 78-79 5.919963135839787 2315 80-81 3.230318598564658 2315 82-83 1.6418588021627478 2315 84-85 0.9961868813273789 2315 86-87 0.9004275262284516 2315 88-89 1.1278976249380221 2315 90-91 2.6126657907893787 2315 92-93 4.2510109446203685 2315 94-95 8.073680673379378 2315 96-97 8.795363861848466 2315 98-99 9.037249579558036 2315 100-101 7.980879994040002 2316 1 -0.509175399429509 2316 2 -0.5320524241388185 2316 3 -0.5673826955452768 2316 4 -0.48781772073461127 2316 5 -0.7710298220572014 2316 6 -0.5668616492653484 2316 7 -0.6606039114459676 2316 8 -0.6039864583910699 2316 9 -0.7532272235691266 2316 10-11 -0.7293864868359137 2316 12-13 -0.5745737367036625 2316 14-15 -0.7870688604034939 2316 16-17 -0.8576820840944421 2316 18-19 -0.9305131360032064 2316 20-21 -0.8115243558549281 2316 22-23 -1.0625874761489058 2316 24-25 -2.0837290117275984 2316 26-27 -1.4564753142324065 2316 28-29 -1.0744017083536548 2316 30-31 -0.9365328659662708 2316 32-33 -0.8207147321858628 2316 34-35 -0.994456482004928 2316 36-37 -1.1006624347626328 2316 38-39 -1.3523928759685617 2316 40-41 -1.1786810259960063 2316 42-43 -1.2115069120861506 2316 44-45 -1.1463611375581664 2316 46-47 -0.986966132894949 2316 48-49 -1.0139346713710466 2316 50-51 -1.195467802254285 2316 52-53 -1.2024722033292505 2316 54-55 -1.164323660989048 2316 56-57 0.24871550416054689 2316 58-59 3.4219990495340404 2316 60-61 3.524457032388632 2316 62-63 0.8729662470369455 2316 64-65 0.6506968165125819 2316 66-67 5.383790893213668 2316 68-69 8.242148234793259 2316 70-71 5.412101775903217 2316 72-73 8.65456825738902 2316 74-75 9.569093465588129 2316 76-77 8.061854272092493 2316 78-79 5.708521885377699 2316 80-81 2.599207694642569 2316 82-83 1.3163716080800683 2316 84-85 1.1659617104782214 2316 86-87 0.9675850673091269 2316 88-89 1.1283737780267202 2316 90-91 2.411985011278844 2316 92-93 4.161203990266287 2316 94-95 7.873789774756968 2316 96-97 8.389714798615467 2316 98-99 8.84589116108637 2316 100-101 7.934783762372142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 22.0 4 42.0 5 125.0 6 287.0 7 705.0 8 1570.0 9 2842.0 10 4579.0 11 7054.0 12 9242.0 13 12412.0 14 16013.0 15 21490.0 16 28650.0 17 35950.0 18 36780.0 19 35411.0 20 35654.0 21 36139.0 22 34001.0 23 32774.0 24 33108.0 25 35079.0 26 45191.0 27 65233.0 28 88960.0 29 115332.0 30 133483.0 31 141401.0 32 143613.0 33 147110.0 34 145231.0 35 134262.0 36 104895.0 37 57114.0 38 14875.0 39 556.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34793923580279 19.0032126154925 14.97531909855512 22.67352905014959 2 16.327326224969198 20.750447474083497 38.42796449801803 24.494261802929284 3 19.42366408955487 24.9007048405501 29.974703138916475 25.70092793097855 4 13.547205223797782 17.03874768166657 35.65973836622014 33.75430872831551 5 13.731723039846564 37.83493049117444 32.98707999152637 15.446266477452628 6 32.32820411259587 36.04835455759689 16.56881140140463 15.054629928402614 7 28.65121509410769 33.68590402056922 19.452432265396606 18.210448619926485 8 27.854266909858666 33.45413260413673 18.922538036689364 19.76906244931524 9 27.502562331086754 15.968572532607478 19.091799459249973 37.4370656770558 10-11 25.631704956040586 26.232010330135232 26.05382801736182 22.08245669646236 12-13 26.986966114629674 23.74590894889508 26.797914168595412 22.469210767879837 14-15 23.380949210300326 24.808201972812228 25.637339679840565 26.173509137046878 16-17 22.353030891953 28.057653445360742 26.460016537777097 23.12929912490916 18-19 22.487004642703077 25.593683237667925 29.652605388449356 22.26670673117964 20-21 23.25503972537957 24.67859177356562 29.919490732072646 22.146877768982165 22-23 22.82776639280123 24.78748728793545 30.078067868624263 22.306678450639062 24-25 22.588975915510513 25.252861246001423 29.88722328673808 22.270939551749983 26-27 22.086655051501392 25.56753399114664 29.651365777830513 22.694445179521455 28-29 22.205958554014458 25.908393371214654 29.531950662278135 22.353697412492753 30-31 22.22926372847092 25.590680245549052 29.329179382166163 22.850876643813862 32-33 21.962327223557693 25.583006674315268 29.316138776587998 23.138527325539044 34-35 22.828009202283784 25.72795215040071 29.200690313557722 22.243348333757783 36-37 23.07961163169506 25.13791924733077 29.075566057363815 22.70690306361035 38-39 22.516480707852164 25.338508416958348 29.06951525856539 23.075495616624096 40-41 22.673106359675963 25.47280169908619 29.3043127803182 22.549779160919652 42-43 22.570254536698467 25.511306274856597 28.825557443879905 23.092881744565027 44-45 22.37076209979834 25.636985414351116 29.14343290018031 22.84881958567023 46-47 22.986191400835335 26.007466446248245 28.203424796730616 22.802917356185805 48-49 22.66016077628938 26.298761089553523 27.772434633932658 23.26864350022443 50-51 22.80770561903157 26.51783640644283 27.775048612660697 22.899409361864905 52-53 23.204446463252758 26.034914379000146 27.78612623319968 22.97451292454742 54-55 22.426511916867234 26.43869132031097 27.983058801565598 23.1517379612562 56-57 22.640969596106114 26.333664853281054 27.806639328144904 23.218726222467932 58-59 21.044091943409292 25.772831356310107 29.51488968769581 23.66818701258479 60-61 22.168656678928706 26.099102153962782 28.382021843324495 23.350219323784014 62-63 22.192471295651586 26.375818320418237 28.059964209730186 23.371746174199988 64-65 21.333944691641165 26.281843399182588 28.51918112943223 23.865030779744014 66-67 21.618218746797126 25.90905350804129 29.062804498676986 23.409923246484595 68-69 22.644251224466743 24.932302453966546 29.601311638125527 22.822134683441185 70-71 21.658414833044432 25.400028637917764 29.413731765155106 23.527824763882695 72-73 21.429514092830676 25.695287403830118 29.297280051048897 23.577918452290312 74-75 22.073598032882824 25.9765468002829 28.569404550346366 23.38045061648791 76-77 20.4874743924558 26.163556730315936 29.183217913509672 24.165750963718594 78-79 21.564926398934638 26.481351824809174 27.941599247843126 24.01212252841307 80-81 22.356461511889325 26.637483635051623 26.928773313915915 24.077281539143137 82-83 22.463700484752323 26.64308160889058 26.719112262446888 24.174105643910206 84-85 22.350782983503766 26.72165600854547 26.613301130384947 24.314259877565817 86-87 22.57829804476841 26.632802771789482 26.295890120947092 24.49300906249502 88-89 22.24410692304584 26.67573038529151 26.206145155700383 24.874017535962267 90-91 21.926329208506708 26.6280896985806 26.603620226955776 24.84196086595692 92-93 21.927597166597778 26.32458703305161 26.87305274859969 24.87476305175092 94-95 20.42432600998662 25.924722343247957 28.47950759521009 25.17144405155533 96-97 20.355122801817764 25.52042218476696 28.73435100682325 25.390104006592022 98-99 20.592473500386983 25.897945007768463 27.834685195982704 25.67489629586184 100-101 20.51046657049131 25.86808546749196 27.7805350332885 25.840912928728233 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 683.0 1 508.5 2 338.0 3 397.0 4 461.5 5 506.0 6 655.0 7 968.0 8 1399.5 9 1782.0 10 1935.0 11 2024.5 12 2293.5 13 2562.5 14 2794.0 15 3052.0 16 3217.0 17 3380.5 18 3694.0 19 4144.0 20 4575.5 21 5081.0 22 5916.0 23 7010.5 24 8113.5 25 9453.0 26 11220.0 27 13090.0 28 15032.5 29 17240.5 30 19639.0 31 22570.5 32 25776.0 33 27699.5 34 28655.0 35 30137.0 36 32207.5 37 34291.5 38 36483.5 39 38679.0 40 41220.0 41 44308.5 42 47575.0 43 50748.0 44 53905.5 45 57175.5 46 60229.5 47 63514.0 48 65965.5 49 67288.5 50 68127.5 51 68636.0 52 69211.5 53 69791.5 54 69290.0 55 66786.0 56 62065.0 57 56081.0 58 49798.0 59 42671.5 60 35523.5 61 29055.5 62 23020.5 63 17326.5 64 12739.5 65 9490.5 66 6990.5 67 5033.0 68 3605.0 69 2655.0 70 1972.5 71 1526.0 72 1246.0 73 1038.0 74 797.0 75 540.0 76 336.5 77 208.5 78 136.5 79 88.0 80 58.5 81 34.0 82 19.0 83 12.5 84 8.0 85 6.0 86 5.5 87 3.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020771812252409958 2 0.005918543765070235 3 0.003073090031863391 4 0.0 5 0.06561616308774981 6 1.1381814932827373E-4 7 0.0 8 2.2763629865654746E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.1381814932827373E-4 16-17 0.0 18-19 0.001308908717275148 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.9918176132447903E-4 28-29 0.001963363075912722 30-31 0.00836563397562812 32-33 2.8454537332068435E-4 34-35 9.389997319582584E-4 36-37 0.02586517443485021 38-39 0.02492617470289195 40-41 0.027316355838785698 42-43 0.027743173898766724 44-45 0.028255355570743955 46-47 0.030161809571992538 48-49 0.029848809661339785 50-51 0.025324538225540906 52-53 0.0214547211483796 54-55 0.016418268040603486 56-57 0.15046759341197788 58-59 0.7749024151642196 60-61 0.3096422752475687 62-63 4.268180599810265E-4 64-65 0.1494432300680234 66-67 1.4424458609745452 68-69 2.75929339416534 70-71 2.2300959088635315 72-73 1.8980030036609605 74-75 1.5910639094599384 76-77 1.132945858413637 78-79 0.4298911500128899 80-81 1.9918176132447903E-4 82-83 1.707272239924106E-4 84-85 0.0 86-87 0.0032438172558558014 88-89 0.0 90-91 0.2267257534619213 92-93 0.17721485850412222 94-95 1.318497896356055 96-97 1.3073152631845522 98-99 1.0304526149435262 100-101 0.4131883365989657 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1757189.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.93813500294157 #Duplication Level Percentage of deduplicated Percentage of total 1 71.36055538013999 32.781708269377596 2 13.98949444645298 12.853025690081168 3 5.637842365572289 7.769758911448898 4 2.844789008788372 5.227372061624182 5 1.6143538918815952 3.7080203513890435 6 1.0419071416824295 2.871796256108185 7 0.688341879362705 2.2134799527639726 8 0.47442534550166254 1.7435372456378058 9 0.3468682694566914 1.4341033251484385 >10 1.8271771369654866 14.889005958732643 >50 0.1061677120908997 3.3529041597618403 >100 0.059025710800762285 5.213750735214721 >500 0.004835845765206035 1.6049463976155345 >1k 0.0038438774031124886 2.914148839509183 >5k 2.4799209052338636E-4 0.8233210970732159 >10k+ 1.2399604526169318E-4 0.5991207485136354 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10518 0.5985696473173916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8407 0.4784345907013986 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6047 0.34412917449403563 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2671 0.15200413842790958 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2633 0.14984159359067237 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2515 0.1431263227803042 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2422 0.1378337788365395 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2375 0.13515905232732506 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2315 0.13174450784747685 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1867 0.10624924239794353 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1853 0.10545251535264563 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 1841 0.10476960645667598 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.707272239924106E-4 2 0.0 0.0 0.0 0.0 1.707272239924106E-4 3 0.0 0.0 0.0 0.0 1.707272239924106E-4 4 0.0 0.0 0.0 0.0 1.707272239924106E-4 5 0.0 0.0 0.0 0.0 1.707272239924106E-4 6 0.0 0.0 0.0 0.0 3.414544479848212E-4 7 0.0 0.0 0.0 0.0 3.414544479848212E-4 8 0.0 0.0 0.0 0.0 3.414544479848212E-4 9 0.0 0.0 0.0 0.0 3.9836352264895807E-4 10-11 0.0 0.0 0.0 0.0 4.5527259731309493E-4 12-13 0.0 0.0 0.0 0.0 0.0014511814039354903 14-15 0.0 0.0 0.0 0.0 0.0019064540012485851 16-17 0.0 0.0 0.0 0.0 0.0023332720612296116 18-19 0.0 0.0 0.0 0.0 0.0024755447478899536 20-21 0.0 0.0 0.0 5.6909074664136866E-5 0.0027031810465465013 22-23 0.0 0.0 0.0 4.837271346451634E-4 0.0031299991065275277 24-25 0.0 0.0 0.0 0.0013658177919392848 0.0031869081811916646 26-27 0.0 0.0 0.0 0.003158453643859596 0.0033291808678520066 28-29 0.0 0.0 0.0 0.0107273605741898 0.0034429990171802806 30-31 0.0 0.0 0.0 0.03428771748514246 0.0035568171665085542 32-33 0.0 0.0 0.0 0.07765243237921476 0.004068998838485786 34-35 0.0 0.0 0.0 0.1388012331058298 0.004381998749138539 36-37 0.0 0.0 0.0 0.2412091129639441 0.004381998749138539 38-39 0.0 0.0 0.0 0.4049365207726659 0.004410453286470608 40-41 0.0 0.0 0.0 0.5939315577322644 0.004495816898466813 42-43 0.0 0.0 0.0 0.7727398703269825 0.004581180510463018 44-45 0.0 0.0 0.0 0.9857505367948468 0.004751907734455428 46-47 0.0 0.0 0.0 1.2281831948640698 0.0050364531077761125 48-49 0.0 0.0 0.0 1.4661769451094901 0.005320998481096797 50-51 0.0 0.0 0.0 1.726820507071237 0.0054063620930930026 52-53 0.0 0.0 0.0 1.959607076984889 0.00563399839174955 54-55 0.0 0.0 0.0 2.202580371263421 0.0058331801530740295 56-57 0.0 0.0 0.0 2.41069685731017 0.005890089227738166 58-59 0.0 0.0 0.0 2.553424816567825 0.005918543765070235 60-61 0.0 0.0 0.0 2.6654503300441785 0.005946998302402303 62-63 0.0 0.0 0.0 2.7565048495067974 0.006089270989062645 64-65 0.0 0.0 0.0 2.846051278490817 0.006146180063726782 66-67 0.0 0.0 0.0 2.924671165139322 0.006231543675722987 68-69 0.0 0.0 0.0 3.0154411392286202 0.0062599982130550555 70-71 0.0 0.0 0.0 3.189611362238211 0.006316907287719192 72-73 0.0 0.0 0.0 3.4127802985336237 0.006601452661039876 74-75 0.0 0.0 0.0 3.6613591366665736 0.006885998034360561 76-77 0.0 0.0 0.0 4.013284854389596 0.007028270721020903 78-79 0.0 0.0 0.0 4.512690439104729 0.007170543407681245 80-81 0.0 0.0 0.0 5.076431732727669 0.007398179706337793 82-83 0.0 0.0 0.0 5.623441758399353 0.007853452303650888 84-85 0.0 0.0 0.0 6.0612148152532255 0.007853452303650888 86-87 0.0 0.0 0.0 6.440627616039025 0.008052634064975367 88-89 0.0 2.8454537332068433E-5 0.0 6.819528235152849 0.008251815826299845 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4005 0.0 62.52447 1 GTATCAA 8530 0.0 47.54528 1 TATCAAC 11425 0.0 35.327564 2 ATCAACG 11655 0.0 34.667908 3 TCAACGC 11870 0.0 34.000248 4 CAACGCA 12095 0.0 33.367752 5 AACGCAG 12495 0.0 32.315205 6 GTGGTAT 1590 0.0 28.472925 1 TGGTATC 1545 0.0 28.382498 2 AGGTAAG 1150 0.0 27.880926 2 ACGCAGA 14460 0.0 27.826075 7 CGCAGAG 14645 0.0 27.442394 8 GGTAAGG 1380 0.0 26.306961 3 TAAGGTG 1455 0.0 25.599007 5 GCAGAGT 15430 0.0 25.343964 9 GTAAGGT 1400 0.0 25.25761 4 GTACATG 10095 0.0 23.544106 1 TACATGG 10075 0.0 22.932276 2 GAGTACT 9225 0.0 22.523432 12-13 AAGGTAA 1385 0.0 22.47254 1 >>END_MODULE