FastQCFastQC Report
Fri 27 May 2016
SRR939892_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939892_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883511
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104780.5563015028847721No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72210.3833797625816892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51900.27554922694903294No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24180.12837726989648587No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23910.1269437768083117No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21610.11473253939053184No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA21510.1142016160245414No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20810.11048515246260839No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG19760.1049104571197089No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45950.056.512571
GTATCAA96350.047.3111571
TATCAAC124700.036.2533762
ATCAACG128700.035.2756423
TCAACGC130650.034.8931774
CAACGCA132650.034.0506025
AACGCAG137600.032.9975136
ACGCAGA154450.029.1234197
CGCAGAG155300.028.9034258
GCAGAGT155750.027.097979
AGGTAAG9550.027.092612
TGGTATC20900.026.7850342
GTGGTAT21700.026.4479261
TACATGG106900.026.359712
GTACATG107400.026.1931921
ACATGGG112650.024.1391983
GAGTACT93100.023.12045912-13
CATGGGA61500.022.8729524
GTAAGGT12400.022.7643744
TAAGGTG12800.022.4223275
Produced by FastQC (version 0.11.5)