##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939892_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1883511 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.985513225035586 31.0 30.0 33.0 26.0 34.0 2 30.01242997784457 31.0 30.0 33.0 25.0 34.0 3 29.94091460044566 31.0 30.0 34.0 25.0 34.0 4 33.61315490060849 35.0 33.0 37.0 28.0 37.0 5 33.47581033506043 35.0 33.0 37.0 28.0 37.0 6 33.48410441988393 35.0 33.0 37.0 28.0 37.0 7 33.456467204067295 35.0 33.0 37.0 28.0 37.0 8 33.28339149598808 35.0 33.0 37.0 26.0 37.0 9 34.57425202188891 37.0 34.0 39.0 27.0 39.0 10-11 34.34891858874198 37.0 33.0 39.0 26.5 39.0 12-13 34.22160422742421 37.0 33.0 39.0 26.0 39.0 14-15 34.74936249376829 38.0 33.0 40.0 25.0 41.0 16-17 34.56232562485698 38.0 33.0 40.0 25.0 41.0 18-19 34.622967426258725 38.0 33.0 40.0 25.0 41.0 20-21 34.741023811382036 38.0 33.0 40.0 25.0 41.0 22-23 34.650356169940075 38.0 33.0 40.0 25.0 41.0 24-25 34.41606499776216 38.0 33.0 40.0 24.0 41.0 26-27 34.27685211288917 38.0 33.0 40.0 23.5 41.0 28-29 34.1174367975552 38.0 33.0 40.0 22.5 41.0 30-31 33.850259701164475 38.0 32.5 40.0 21.5 41.0 32-33 33.468169816900456 37.0 32.0 40.0 19.0 41.0 34-35 33.14371750417173 37.0 31.0 40.0 18.0 41.0 36-37 32.8512756230253 37.0 31.0 40.0 16.0 41.0 38-39 32.495013833208304 37.0 30.0 40.0 15.0 41.0 40-41 32.120962394167066 36.5 30.0 39.5 12.0 41.0 42-43 31.708555989319947 36.0 30.0 39.0 9.0 41.0 44-45 31.197021944655486 36.0 29.5 39.0 8.0 40.0 46-47 30.676988878748254 35.0 28.5 39.0 4.0 40.0 48-49 30.21507493186926 35.0 27.5 39.0 2.0 40.0 50-51 29.40250893145832 34.5 26.0 38.5 2.0 39.5 52-53 29.467690658562653 34.5 26.5 38.0 2.0 40.0 54-55 29.49638042995236 35.0 26.0 38.0 2.0 40.0 56-57 29.22196021154111 34.5 26.0 38.0 2.0 40.0 58-59 28.646553165869484 34.0 25.5 38.0 2.0 40.0 60-61 28.129018359860922 34.0 24.0 38.0 2.0 40.0 62-63 27.649879400757413 33.0 23.0 37.0 2.0 39.5 64-65 27.086623332701535 33.0 22.0 36.5 2.0 39.0 66-67 26.534468872228516 32.0 20.5 36.0 2.0 39.0 68-69 25.96593117852776 32.0 19.5 35.5 2.0 38.5 70-71 25.389837648943914 31.0 17.5 35.0 2.0 38.0 72-73 24.907863293604336 31.0 14.0 35.0 2.0 37.5 74-75 24.318983536597344 31.0 9.0 35.0 2.0 37.0 76-77 23.685185539134096 30.0 7.0 34.0 2.0 36.0 78-79 23.033834153344472 30.0 2.0 34.0 2.0 36.0 80-81 22.37770472272262 29.0 2.0 34.0 2.0 35.0 82-83 21.644778819980345 29.0 2.0 34.0 2.0 35.0 84-85 20.901297098875453 27.5 2.0 33.5 2.0 35.0 86-87 20.118812685458167 26.5 2.0 33.0 2.0 35.0 88-89 19.26062921851797 25.5 2.0 32.5 2.0 34.0 90-91 18.327330979219127 24.5 2.0 32.0 2.0 34.0 92-93 17.37183164844803 21.5 2.0 32.0 2.0 34.0 94-95 16.038884296401772 10.0 2.0 31.0 2.0 34.0 96-97 13.661534761411003 2.0 2.0 30.0 2.0 34.0 98-99 12.369666808423204 2.0 2.0 29.0 2.0 34.0 100-101 11.164029039384427 2.0 2.0 26.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7695807026543662 1101 2 0.7449791994932902 1101 3 0.8674804910700082 1101 4 0.9662865852141067 1101 5 1.013356634908476 1101 6 1.0965885423147483 1101 7 0.9401456417549028 1101 8 0.6805338380822903 1101 9 0.8637877339693887 1101 10-11 1.0096805593372977 1101 12-13 0.908287189159843 1101 14-15 1.019159837885546 1101 16-17 0.9752386526624264 1101 18-19 0.8167447639519665 1101 20-21 0.8391181476627949 1101 22-23 0.8297181918849219 1101 24-25 1.012055939879005 1101 26-27 0.9679307320473214 1101 28-29 1.1302902043373706 1101 30-31 1.2759233887018198 1101 32-33 1.2059538101870615 1101 34-35 0.6713203206777294 1101 36-37 0.7179561962745993 1101 38-39 0.7347087680517106 1101 40-41 0.9015522532269102 1101 42-43 0.7854441795468041 1101 44-45 0.8178702102720585 1101 46-47 0.6908401322538644 1101 48-49 0.41869582969600216 1101 50-51 0.5206385921122987 1101 52-53 0.6405076562424021 1101 54-55 0.4599192246288091 1101 56-57 0.10269915411695862 1101 58-59 0.10849755438315611 1101 60-61 0.3400087153300433 1101 62-63 0.26675267620218435 1101 64-65 0.46778453232312245 1101 66-67 0.11685579106221766 1101 68-69 0.16996960428216923 1101 70-71 0.09778026510968019 1101 72-73 -0.22745858498291227 1101 74-75 -0.1431093403376842 1101 76-77 -0.2195738992446543 1101 78-79 -0.20907864778783036 1101 80-81 -0.5258062706368207 1101 82-83 -0.6139629696212126 1101 84-85 -0.4822748621025532 1101 86-87 -0.3860724788439178 1101 88-89 -0.6105933961728987 1101 90-91 -0.5040817469197663 1101 92-93 -0.7154865263760541 1101 94-95 -0.2566242096527791 1101 96-97 0.9319786998355237 1101 98-99 0.822455099031524 1101 100-101 0.2233295089601075 1102 1 0.9100215626074224 1102 2 0.8625800945332109 1102 3 1.0515017543595633 1102 4 0.9013246162887256 1102 5 1.1481730380175605 1102 6 0.9777388960260751 1102 7 0.7729139912915457 1102 8 0.7242299793394054 1102 9 1.0113474912852496 1102 10-11 0.9646607925187567 1102 12-13 0.8891361166551164 1102 14-15 1.0139615300957772 1102 16-17 0.8900486901858784 1102 18-19 1.1534971883568303 1102 20-21 1.088653673112951 1102 22-23 0.8886323468356636 1102 24-25 1.1581819175598582 1102 26-27 1.2234394509706803 1102 28-29 1.1024646254447106 1102 30-31 1.096149910147851 1102 32-33 1.287327213206808 1102 34-35 1.233091628638057 1102 36-37 0.9962906666822349 1102 38-39 0.8890089901872997 1102 40-41 1.0288233561077718 1102 42-43 1.081624878928725 1102 44-45 0.8236378166494198 1102 46-47 1.037510484259581 1102 48-49 0.8116928225405573 1102 50-51 0.8622248513814839 1102 52-53 0.8949260021241621 1102 54-55 0.7539666406274499 1102 56-57 0.6891967520474829 1102 58-59 0.5493869495408461 1102 60-61 0.7909518270963289 1102 62-63 0.4828475569312083 1102 64-65 0.4194459298686688 1102 66-67 0.24976404134851649 1102 68-69 0.1639603696143972 1102 70-71 0.36312416917309775 1102 72-73 0.3383904541892946 1102 74-75 0.13074561568190646 1102 76-77 -0.013763703342998923 1102 78-79 -0.05434009365903236 1102 80-81 -0.31053449972480607 1102 82-83 -0.2849454424329565 1102 84-85 -0.44224121688598217 1102 86-87 -0.42027988728913 1102 88-89 -0.7004220126783416 1102 90-91 -0.6209559804098532 1102 92-93 -0.8407418604728534 1102 94-95 -0.6180389635980195 1102 96-97 0.09971594226788838 1102 98-99 0.33305425597214544 1102 100-101 0.008019158741220167 1103 1 0.7604848261551744 1103 2 0.621878543243465 1103 3 0.6567630317337638 1103 4 0.6658715306554086 1103 5 0.7175033169557068 1103 6 0.7715544603745883 1103 7 0.5131472258062146 1103 8 0.6951131813909157 1103 9 0.7809203251351349 1103 10-11 0.6220378153409598 1103 12-13 0.6304917801489651 1103 14-15 0.8396087260139993 1103 16-17 0.7026562626767259 1103 18-19 0.8248402596165221 1103 20-21 0.5730154658142581 1103 22-23 0.8043595726036585 1103 24-25 0.7240442278341419 1103 26-27 1.0705063332417097 1103 28-29 1.0520355860275004 1103 30-31 0.8343815051709811 1103 32-33 0.7688260208493318 1103 34-35 0.7679791866736068 1103 36-37 0.5938592986970335 1103 38-39 0.5620168191166641 1103 40-41 0.7944107759196868 1103 42-43 0.5265249220087647 1103 44-45 0.5359228758385335 1103 46-47 0.427149403093523 1103 48-49 0.5525274676712861 1103 50-51 0.746392120352013 1103 52-53 0.7766518987402371 1103 54-55 0.7245813580754721 1103 56-57 0.6946127653675873 1103 58-59 0.5703313007472914 1103 60-61 0.5167380633301093 1103 62-63 0.4015212225729421 1103 64-65 0.3831398951922971 1103 66-67 0.42859239699771834 1103 68-69 0.1170832641030124 1103 70-71 0.5347121177873895 1103 72-73 0.18062780376645549 1103 74-75 0.28203043701203967 1103 76-77 -0.007762230250666136 1103 78-79 -0.10623673831608826 1103 80-81 -0.16888832670230514 1103 82-83 -0.21583209497099887 1103 84-85 -0.37438275421044764 1103 86-87 -0.5190831146169828 1103 88-89 -0.23621915163290552 1103 90-91 -0.4077747390105486 1103 92-93 -0.381749821092388 1103 94-95 -0.29379008245401117 1103 96-97 1.0921210436795246 1103 98-99 1.2829752418721103 1103 100-101 1.063263056075975 1104 1 0.488742871171052 1104 2 0.539604137613761 1104 3 0.6111593544112353 1104 4 0.7491030735916695 1104 5 0.753969051290504 1104 6 0.8957992620815745 1104 7 0.6907932067023737 1104 8 0.7068798852823619 1104 9 0.8809795788672972 1104 10-11 0.6139533967283057 1104 12-13 0.7804361424905295 1104 14-15 0.743220285878472 1104 16-17 0.8353333742268632 1104 18-19 0.8768279280569828 1104 20-21 0.7615576743724333 1104 22-23 0.6785657197294697 1104 24-25 0.8564260682610438 1104 26-27 1.0558725816574963 1104 28-29 1.1951615296356977 1104 30-31 1.1200517032869257 1104 32-33 1.1901714297075614 1104 34-35 0.9559924509307862 1104 36-37 0.8756443373291773 1104 38-39 0.6692558495699856 1104 40-41 0.6209629319225911 1104 42-43 0.6320677645020716 1104 44-45 0.7441041054731805 1104 46-47 0.49830600302658823 1104 48-49 0.43061216559446436 1104 50-51 0.6230262739438714 1104 52-53 0.5369481075487919 1104 54-55 0.38901520312895954 1104 56-57 0.44692593031990313 1104 58-59 0.23826500639490078 1104 60-61 0.47569899327454124 1104 62-63 0.1852599385525444 1104 64-65 0.3511387649822204 1104 66-67 0.32404135624182473 1104 68-69 0.18671874392108023 1104 70-71 0.18276826618507158 1104 72-73 0.265793197990817 1104 74-75 0.1890455950943135 1104 76-77 -0.0140626775963959 1104 78-79 0.06499831794752708 1104 80-81 0.16540147891253554 1104 82-83 0.050560689440679596 1104 84-85 -0.03548232990783973 1104 86-87 -0.14318676475661363 1104 88-89 -0.25456511462326503 1104 90-91 -0.031140834803082384 1104 92-93 -0.14302274388247938 1104 94-95 0.2703220317919719 1104 96-97 1.5050074585643838 1104 98-99 1.5453607931388067 1104 100-101 1.2135708961726959 1105 1 0.7860965895590866 1105 2 0.8721600943710968 1105 3 0.7637532113706449 1105 4 0.6436864535411928 1105 5 0.7213316110788668 1105 6 0.9639216080525941 1105 7 0.9922899082561045 1105 8 0.8108198033632732 1105 9 0.9253737053260522 1105 10-11 0.824653223761608 1105 12-13 0.627215070849445 1105 14-15 0.8183053583670628 1105 16-17 0.7599230609759502 1105 18-19 0.8346921704836205 1105 20-21 0.8729820903004111 1105 22-23 0.5385216018348942 1105 24-25 0.7361439157117431 1105 26-27 0.9080461998297906 1105 28-29 1.0198134428137422 1105 30-31 0.837916480355922 1105 32-33 0.8435766559355358 1105 34-35 1.154675201091429 1105 36-37 0.8273918752516565 1105 38-39 0.8994109651566973 1105 40-41 0.7117704497860871 1105 42-43 0.787122461384616 1105 44-45 0.754217014016664 1105 46-47 0.7401482518511138 1105 48-49 0.6987721840495276 1105 50-51 0.8430395211931909 1105 52-53 0.9204064712857836 1105 54-55 0.8268516441534537 1105 56-57 0.48110937799079423 1105 58-59 0.30239114832660263 1105 60-61 0.7057083198132972 1105 62-63 0.7125608560582215 1105 64-65 0.7447993918404769 1105 66-67 0.7216699699043119 1105 68-69 0.6880375283820008 1105 70-71 0.6695093834489683 1105 72-73 0.7753511675775862 1105 74-75 0.5999880408454423 1105 76-77 0.5446167954046643 1105 78-79 0.32930470365633724 1105 80-81 0.13514294963443163 1105 82-83 0.016952724853801726 1105 84-85 -0.40395313398022736 1105 86-87 -0.2368249168346388 1105 88-89 -0.18777170141700594 1105 90-91 -0.21843817812564836 1105 92-93 -0.24382782615304777 1105 94-95 0.14421821810889135 1105 96-97 1.4719554230126466 1105 98-99 1.6426498594057453 1105 100-101 1.1979632998949956 1106 1 0.852511091391893 1106 2 1.1039816781015261 1106 3 1.0588251080938988 1106 4 1.0263535826812102 1106 5 0.9053751119029769 1106 6 1.016438537817379 1106 7 0.9794137790518107 1106 8 0.9575442544107702 1106 9 1.2066643565476625 1106 10-11 1.0450567441540812 1106 12-13 1.2888590680002423 1106 14-15 1.3250017051285923 1106 16-17 1.503526427753613 1106 18-19 1.2495081912545487 1106 20-21 1.3804895359974196 1106 22-23 1.0070017500954975 1106 24-25 1.1211316004996803 1106 26-27 1.4426971749810562 1106 28-29 1.3600515192079001 1106 30-31 1.4253283257998746 1106 32-33 1.429658049474682 1106 34-35 1.5351007863495099 1106 36-37 1.4258016273485836 1106 38-39 1.3524397318135684 1106 40-41 1.298034300747851 1106 42-43 1.231924044913196 1106 44-45 1.4421428475974416 1106 46-47 1.3160384555075915 1106 48-49 1.186524032393681 1106 50-51 1.4164786337967747 1106 52-53 1.4863883339025143 1106 54-55 1.4084002853901048 1106 56-57 1.282192542987012 1106 58-59 1.1442835584696418 1106 60-61 1.0901184883487147 1106 62-63 1.125716301814716 1106 64-65 1.1225559729230135 1106 66-67 1.2205244984869168 1106 68-69 1.1654368434894202 1106 70-71 1.440271418069667 1106 72-73 1.3649035015187572 1106 74-75 1.232667386930789 1106 76-77 1.1658394354228072 1106 78-79 0.8470447706158204 1106 80-81 0.9289064380716248 1106 82-83 1.079083062662967 1106 84-85 1.0868949132472956 1106 86-87 0.9852879135606933 1106 88-89 1.2375632420743123 1106 90-91 1.2720071082847006 1106 92-93 1.3873479061067542 1106 94-95 1.4380696154352943 1106 96-97 2.797233982645418 1106 98-99 2.9633932764641084 1106 100-101 2.5961420986080714 1107 1 0.5808402891433104 1107 2 0.6459737197538686 1107 3 0.5177196504834782 1107 4 0.3308934360594904 1107 5 0.6576084076006126 1107 6 0.7205939965954258 1107 7 0.6959998792605191 1107 8 0.6241951716518841 1107 9 0.741252267397229 1107 10-11 0.7299396580367699 1107 12-13 0.6831631796321673 1107 14-15 0.698385556102096 1107 16-17 0.8153154973264307 1107 18-19 0.8646423301981514 1107 20-21 0.7953818126420558 1107 22-23 0.7267128893383301 1107 24-25 0.705631477491238 1107 26-27 1.0006951134355546 1107 28-29 0.862789611148699 1107 30-31 0.7600816870802944 1107 32-33 0.8788823088036395 1107 34-35 1.0591394332674824 1107 36-37 0.813889060556086 1107 38-39 0.8264895662545229 1107 40-41 0.804214321026592 1107 42-43 0.8964705130502075 1107 44-45 1.0000226653602944 1107 46-47 0.771308593456272 1107 48-49 0.627497221983635 1107 50-51 0.7470082978398018 1107 52-53 0.9023474983574751 1107 54-55 0.8084593185817504 1107 56-57 0.7559055298003976 1107 58-59 0.8342847641153242 1107 60-61 0.879287988554271 1107 62-63 0.7418229677862378 1107 64-65 0.8855123193169625 1107 66-67 0.7957179584564962 1107 68-69 0.4918643252594599 1107 70-71 0.6888169043114587 1107 72-73 0.8302052089194092 1107 74-75 0.7911243668318058 1107 76-77 0.7525640545446741 1107 78-79 0.6354830087004295 1107 80-81 0.808984066447092 1107 82-83 0.7959917545536968 1107 84-85 0.6277486515906823 1107 86-87 0.6527113672793803 1107 88-89 0.7157046686572777 1107 90-91 0.4816420094461904 1107 92-93 0.3698990360737291 1107 94-95 0.6644261976361587 1107 96-97 1.8779863109807504 1107 98-99 2.072655459323629 1107 100-101 1.5548005353852616 1108 1 0.4626339026843098 1108 2 0.5352419083606534 1108 3 0.5861050189968608 1108 4 0.37133863910337084 1108 5 0.7013084763486859 1108 6 0.7181261778058712 1108 7 0.5331309510192881 1108 8 0.5916844445817375 1108 9 0.6521239455614136 1108 10-11 0.6045182797564195 1108 12-13 0.4630875567509136 1108 14-15 0.7529122611728596 1108 16-17 0.7112652875262739 1108 18-19 0.5046430413851226 1108 20-21 0.35651070342743907 1108 22-23 0.39533887611025875 1108 24-25 0.4889458458386784 1108 26-27 0.3769876957554459 1108 28-29 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2311 92-93 2.109912712066663 2311 94-95 2.154052070588717 2311 96-97 3.4546842551497736 2311 98-99 3.5020943004098566 2311 100-101 3.01485682222655 2312 1 -0.08460371303288028 2312 2 -0.44112059880414023 2312 3 -0.8394754454278761 2312 4 -0.40195634015697124 2312 5 -2.1011145860990297 2312 6 -0.6947570138275196 2312 7 -0.330245829871771 2312 8 0.1544317877908412 2312 9 0.02514310530932562 2312 10-11 0.1523369251022615 2312 12-13 0.2329796094518528 2312 14-15 0.429341395905503 2312 16-17 -0.49060520497246785 2312 18-19 0.04993475975133066 2312 20-21 0.43150967276147156 2312 22-23 0.36301479749238297 2312 24-25 0.3745484396592076 2312 26-27 0.5048676757006874 2312 28-29 0.5052730444514424 2312 30-31 0.22424681000516244 2312 32-33 -0.5640814813356201 2312 34-35 -0.0530084899866452 2312 36-37 0.15212367573286656 2312 38-39 0.49227904131935674 2312 40-41 0.453075099911878 2312 42-43 0.27674225357776905 2312 44-45 0.2987773628209993 2312 46-47 -0.03790577443851717 2312 48-49 0.23648316330938002 2312 50-51 0.10099891629660362 2312 52-53 -0.055149105935203835 2312 54-55 0.0243274103699882 2312 56-57 -0.03579333640847082 2312 58-59 0.11781530816823249 2312 60-61 0.0748245942529735 2312 62-63 0.2007875039580611 2312 64-65 0.17245299818561577 2312 66-67 0.3752606646240615 2312 68-69 0.3690289083337568 2312 70-71 0.43837109476381286 2312 72-73 0.544215957664349 2312 74-75 0.587777503132795 2312 76-77 0.7375123752631332 2312 78-79 0.6817058122194801 2312 80-81 0.5767433927723395 2312 82-83 0.7582840576695631 2312 84-85 0.7119879003537228 2312 86-87 0.8267368425603614 2312 88-89 0.7150449387294593 2312 90-91 0.8300588937056901 2312 92-93 1.0082404671656597 2312 94-95 1.5032439458664317 2312 96-97 2.844754678360344 2312 98-99 2.971537617131027 2312 100-101 2.5223120498366267 2313 1 -0.13812810615912596 2313 2 -0.6413454174993944 2313 3 -0.959088521190747 2313 4 -0.09477045348627655 2313 5 -0.29726646109074295 2313 6 0.13382116765889407 2313 7 0.22174200436126057 2313 8 0.26131786205411345 2313 9 0.34291880805049146 2313 10-11 -0.556646590630038 2313 12-13 0.1359828233520588 2313 14-15 0.11125908529779593 2313 16-17 -0.06760317147870865 2313 18-19 0.06651432632015997 2313 20-21 -2.13024507722384 2313 22-23 -0.25422758353902 2313 24-25 0.24422737181372156 2313 26-27 0.296897793392084 2313 28-29 0.12217010245082349 2313 30-31 0.2249407048943013 2313 32-33 0.4762438830810325 2313 34-35 0.407498792390129 2313 36-37 -0.4662659466534862 2313 38-39 0.3474581964244905 2313 40-41 0.655883140645642 2313 42-43 0.441805309211464 2313 44-45 0.5422941293303758 2313 46-47 0.6390094011753682 2313 48-49 0.23837628075467876 2313 50-51 0.8183289343844393 2313 52-53 0.8244004336385977 2313 54-55 0.7233056278100598 2313 56-57 0.7715337387853154 2313 58-59 0.6776051958211511 2313 60-61 0.4657406898258287 2313 62-63 0.6873323595383134 2313 64-65 0.6283089106276165 2313 66-67 0.8020896786895797 2313 68-69 0.49425026838398267 2313 70-71 0.7801514184171836 2313 72-73 0.7121425818670701 2313 74-75 0.9009479963288065 2313 76-77 0.9896423185196959 2313 78-79 0.9265476932567118 2313 80-81 0.9006432661493804 2313 82-83 0.842138398763165 2313 84-85 0.7244647423224784 2313 86-87 0.8600543559204787 2313 88-89 0.8555149300090754 2313 90-91 1.2716808912836939 2313 92-93 1.2052942972190763 2313 94-95 1.4987570477011243 2313 96-97 2.881607319506225 2313 98-99 2.7527050174455674 2313 100-101 2.320889497630038 2314 1 -0.3642795547353046 2314 2 -0.3716629818547723 2314 3 -1.4653866417971244 2314 4 -0.35698426158614893 2314 5 -0.6863906766035441 2314 6 -0.6497827334682924 2314 7 -0.5201998950612179 2314 8 -1.8567230227039815 2314 9 -1.6003128713076862 2314 10-11 -1.0261806382886007 2314 12-13 -0.6856100900315525 2314 14-15 -0.8029445283364609 2314 16-17 -0.6699690807805965 2314 18-19 -1.9595025181776649 2314 20-21 -1.5527135500512586 2314 22-23 -2.3009599534991025 2314 24-25 -2.0260059981829386 2314 26-27 -2.0388947953664314 2314 28-29 -3.061773614197044 2314 30-31 -2.0245144087948077 2314 32-33 -1.7781943945493737 2314 34-35 -2.16003068126151 2314 36-37 -3.563553470529186 2314 38-39 -3.8580484747055337 2314 40-41 -4.751829658978529 2314 42-43 -3.4507049654258672 2314 44-45 -4.0106248241053315 2314 46-47 -4.018838424483516 2314 48-49 -2.0318359914240496 2314 50-51 -1.588610981843157 2314 52-53 -2.6909050486997117 2314 54-55 -2.2482050040836548 2314 56-57 -2.0045200972199417 2314 58-59 -4.027528184583758 2314 60-61 -2.304687764660553 2314 62-63 -2.2808996714501646 2314 64-65 -1.8163489812221556 2314 66-67 -2.925651701445279 2314 68-69 -2.8851429302421288 2314 70-71 -2.2269920214273107 2314 72-73 -1.9071377293679426 2314 74-75 -2.2736229443806977 2314 76-77 -1.1668442759267954 2314 78-79 -0.9314856525477744 2314 80-81 -0.9632482404317351 2314 82-83 -0.7526566289031074 2314 84-85 -0.6444362301462725 2314 86-87 -0.21007788520226356 2314 88-89 -0.10174342261704439 2314 90-91 0.4135476824743698 2314 92-93 0.6246938814514138 2314 94-95 0.9602383395627125 2314 96-97 2.3697438758359954 2314 98-99 2.505207640607596 2314 100-101 2.286801331255459 2315 1 -2.408037210159076 2315 2 -1.9453103991761935 2315 3 -1.5983983228534981 2315 4 -1.5771766554558369 2315 5 -1.6818855530711438 2315 6 -2.8839236828902166 2315 7 -2.224588506778126 2315 8 -1.3472556908245572 2315 9 -1.254478915582176 2315 10-11 -3.5468913603631123 2315 12-13 -2.0880672943454712 2315 14-15 -1.370534824993463 2315 16-17 -2.1675417512911537 2315 18-19 -1.3400330950605337 2315 20-21 -0.8599581949889696 2315 22-23 -1.1733884779815185 2315 24-25 -3.145813006816759 2315 26-27 -4.522961057727144 2315 28-29 -6.173247040540662 2315 30-31 -8.82294896799177 2315 32-33 -7.223535415670874 2315 34-35 -6.527184805481028 2315 36-37 -4.047117835838542 2315 38-39 -6.339206422902592 2315 40-41 -4.099999825978799 2315 42-43 -4.042171221811611 2315 44-45 -3.2380065944874303 2315 46-47 -2.50543956570181 2315 48-49 -2.7308336910625677 2315 50-51 -2.5173625129279102 2315 52-53 -2.21579525871973 2315 54-55 -3.595679087673414 2315 56-57 -3.5187076513680573 2315 58-59 -2.3897589377280823 2315 60-61 -2.447673155871378 2315 62-63 -3.1022049025319305 2315 64-65 -2.430352805199689 2315 66-67 -2.9298670391801664 2315 68-69 -2.0713407613298713 2315 70-71 -2.6137261056145675 2315 72-73 -1.7387587373445186 2315 74-75 -2.2032363641518593 2315 76-77 -1.2579684655721834 2315 78-79 -1.311783205799653 2315 80-81 -0.9282080587928014 2315 82-83 -0.8258036429144013 2315 84-85 -1.042056700376012 2315 86-87 -1.603783259683187 2315 88-89 -1.7581112414561062 2315 90-91 -1.3451727787929215 2315 92-93 -0.5793466885602356 2315 94-95 0.17618850835547128 2315 96-97 1.39161822232421 2315 98-99 1.2456581529608357 2315 100-101 0.8313656069403397 2316 1 -3.720997469173806 2316 2 -2.4777792920151995 2316 3 -2.5023184599789445 2316 4 -3.5529067214791965 2316 5 -3.0042382701864305 2316 6 -6.032469899243697 2316 7 -3.7646183442590804 2316 8 -4.8833273007788485 2316 9 -6.789483994302337 2316 10-11 -5.770732777326035 2316 12-13 -3.8323346889620282 2316 14-15 -5.0585093679086555 2316 16-17 -4.6149055781068 2316 18-19 -2.831778587696384 2316 20-21 -3.5741373468427007 2316 22-23 -4.599121718204987 2316 24-25 -3.351290089092025 2316 26-27 -4.195128084136492 2316 28-29 -4.09170342703127 2316 30-31 -4.143063874035448 2316 32-33 -3.7330072287739746 2316 34-35 -5.994957151849746 2316 36-37 -6.045221251277848 2316 38-39 -5.977366656523721 2316 40-41 -5.522601717802061 2316 42-43 -4.849886115158487 2316 44-45 -4.334037257260409 2316 46-47 -4.206421980633248 2316 48-49 -4.778978371103197 2316 50-51 -5.988477292003587 2316 52-53 -3.7791570540473067 2316 54-55 -3.888245111035527 2316 56-57 -2.8990053913375853 2316 58-59 -3.6328423557067495 2316 60-61 -3.4772646363183064 2316 62-63 -2.661581489631981 2316 64-65 -3.0853997833611935 2316 66-67 -3.7228107923543696 2316 68-69 -3.0166937323811673 2316 70-71 -2.5846123422829272 2316 72-73 -2.590242675892007 2316 74-75 -3.4186931315464726 2316 76-77 -3.6739632598840153 2316 78-79 -3.6907938076279905 2316 80-81 -3.9765336682392203 2316 82-83 -2.947526281309525 2316 84-85 -2.9022236633013563 2316 86-87 -2.544386040282479 2316 88-89 -2.3805702942747935 2316 90-91 -1.9393433603063812 2316 92-93 -1.6562051827197095 2316 94-95 -1.3284648689934375 2316 96-97 0.05672582770765189 2316 98-99 0.3336501539765795 2316 100-101 0.06978929916989784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17240.0 3 5838.0 4 5822.0 5 6536.0 6 7527.0 7 9232.0 8 11535.0 9 14348.0 10 16865.0 11 19550.0 12 23039.0 13 25790.0 14 28141.0 15 31502.0 16 34732.0 17 36846.0 18 32998.0 19 31498.0 20 33200.0 21 36077.0 22 38821.0 23 43241.0 24 47850.0 25 53250.0 26 59642.0 27 66227.0 28 73762.0 29 82204.0 30 91871.0 31 103468.0 32 116703.0 33 131424.0 34 146549.0 35 153698.0 36 139871.0 37 88963.0 38 17521.0 39 130.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.51132334821993 18.065160147002075 13.980239553423262 22.44327695135473 2 16.337092279588212 19.812678085770305 39.49157758281171 24.35865205182977 3 19.66456113716666 24.789912169492734 29.29678716716714 26.24873952617347 4 13.132876903136353 16.155247228297682 36.06057287341558 34.65130299515039 5 14.067286253653569 37.381328819513485 32.70019000147791 15.851194925355044 6 32.30753445703915 35.528256207476936 16.998327519167606 15.16588181631631 7 29.04363211810466 33.53933313059437 19.47628485816425 17.94074989313672 8 28.05918681136397 32.82416002620033 19.108327325545538 20.008325836890155 9 28.143504672276055 15.983145245975727 19.160771490476 36.71257859127222 10-11 25.811938955843793 25.950598340766074 26.02819549271932 22.20926721067082 12-13 27.355302805753194 23.58932779688096 26.71291305740569 22.34245633996015 14-15 23.92244535709036 24.57977369086537 25.595881557567797 25.901899394476473 16-17 22.753081592526723 27.868526515929815 26.210674525404315 23.16771736613915 18-19 22.859117050283512 25.382198930608666 29.58101681505145 22.17766720405637 20-21 23.592184082281218 24.610753202890745 29.796916448929935 22.000146265898106 22-23 23.17226532015978 24.622026388598524 29.933419405083555 22.272288886158144 24-25 22.764610251844065 25.050113688572324 29.89029125929481 22.294984800288802 26-27 22.210818116034563 25.35454709913591 29.716351679223596 22.718283105605924 28-29 22.40921442523442 25.775845601869957 29.547659067968997 22.267280904926622 30-31 22.329138049560182 25.432129836353074 29.40757534803439 22.83115676605236 32-33 22.019759634391427 25.54793132015918 29.28561280885736 23.14669623659204 34-35 23.01312129605446 25.643737157040107 29.087498889581 22.255642657324433 36-37 23.220735634751573 25.074369264072715 29.045419369250773 22.659475731924932 38-39 22.69720700049178 25.284264319870804 28.981010390731615 23.037518288905797 40-41 22.93897916719855 25.360590220157096 29.217248759648363 22.483181852995994 42-43 22.783628860123113 25.419408336520238 28.781669378417725 23.015293424938925 44-45 22.55171175723566 25.632356167844627 29.063423780426994 22.752508294492724 46-47 23.149092495497726 25.91728049244389 28.174244056596997 22.759382955461387 48-49 22.89419765801283 26.191646204720207 27.673154094752757 23.2410020425142 50-51 22.92652954893138 26.547271185344705 27.651532589122844 22.874666676601063 52-53 23.25335791876905 26.105186842036627 27.64070309094074 23.000752148253582 54-55 22.551633193746397 26.432123358123654 27.896974463175795 23.119268984954154 56-57 22.70253573573158 26.49426001421261 27.539533654921883 23.263670595133924 58-59 22.71386320810309 25.921083050911935 27.59940971511864 23.765644025866333 60-61 22.184780923183805 26.280919327077985 28.02886854486692 23.50543120487129 62-63 22.35841105600261 26.25808598114337 28.037367518630703 23.346135444223318 64-65 22.11965512848913 26.346328287447175 27.79629700052415 23.73771958353954 66-67 22.093536855535703 26.408885823108424 27.744264270748598 23.75331305060727 68-69 22.826257791597 25.95736351956931 27.7068777206931 23.509500968140582 70-71 22.35954921711379 26.16120474718261 27.38991389913899 24.089332136564607 72-73 22.38684147937051 26.41268241655276 27.238681487543236 23.961794616533496 74-75 22.558042634576054 26.75601951703377 27.09254945909889 23.59338838929128 76-77 22.624062667487728 26.27104390992666 27.167008450296425 23.93788497228918 78-79 22.780483002320285 26.560710560487045 26.99720075781766 23.661605679375015 80-81 23.004862991371684 26.58618005707861 26.707548613282416 23.701408338267285 82-83 22.876732486081437 26.575133092130592 26.78177946313986 23.766354958648108 84-85 22.7697260337752 26.709651672464368 26.647049824738772 23.873572469021664 86-87 23.110487310849212 26.560971184648842 26.289199523920747 24.039341980581202 88-89 22.85954626642681 26.571996511788143 26.18013134532925 24.388325876455795 90-91 22.849743992755283 26.653192787847864 26.19318849933411 24.303874720062744 92-93 23.148090503092348 26.1844595669597 26.365748833219726 24.301701096728227 94-95 22.82002550535104 25.97600006598505 26.643380329057877 24.560594099606032 96-97 23.361119576419245 25.410977785739046 26.507732768186777 24.720169869654935 98-99 23.132646614944044 25.74653537146339 26.052499801001993 25.068318212590572 100-101 23.27241803565671 25.680780836189527 26.265225829108612 24.781575299045148 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2862.0 1 1736.5 2 671.5 3 749.0 4 675.5 5 643.0 6 906.5 7 1741.5 8 2823.5 9 3211.5 10 2787.5 11 2320.5 12 2235.5 13 2354.0 14 2550.0 15 2788.0 16 2980.0 17 3183.5 18 3560.5 19 3991.5 20 4484.5 21 5149.0 22 6026.5 23 7159.5 24 8418.0 25 9973.0 26 11980.5 27 14135.0 28 16205.0 29 18165.0 30 20507.0 31 24162.0 32 29171.5 33 30551.5 34 29240.5 35 30114.0 36 32099.5 37 34658.5 38 37567.5 39 39691.5 40 41813.0 41 44751.0 42 47246.0 43 49717.5 44 52613.5 45 57186.5 46 61216.0 47 64371.5 48 67099.5 49 67929.0 50 68678.5 51 69156.0 52 70264.5 53 72809.5 54 74115.5 55 71623.0 56 67150.0 57 62681.0 58 58480.0 59 52177.0 60 43776.0 61 36552.5 62 30775.0 63 24235.5 64 17894.0 65 13627.5 66 10312.0 67 7872.5 68 5881.5 69 4351.5 70 3300.0 71 2515.0 72 2019.5 73 1648.5 74 1336.0 75 1052.0 76 757.5 77 507.0 78 348.5 79 267.5 80 174.5 81 119.0 82 95.0 83 64.5 84 39.0 85 30.0 86 24.5 87 13.0 88 8.0 89 8.5 90 5.5 91 2.5 92 5.0 93 3.5 94 1.5 95 2.5 96 2.0 97 0.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.11558201677611651 2 0.10852073600844381 3 0.12073197342622369 4 0.13469525795177198 5 0.13129734840943325 6 0.13129734840943325 7 0.13819935216730883 8 0.13904882955289352 9 0.14526063293498154 10-11 0.14032304563127054 12-13 0.14034959179957004 14-15 0.1280321697085921 16-17 0.1307398788751433 18-19 0.14945492752630593 20-21 0.17910699751687142 22-23 0.19559216803087426 24-25 0.21420103200883883 26-27 0.2161389022947039 28-29 0.21820950342206655 30-31 0.21762548771947707 32-33 0.21895279613445315 34-35 0.1906014883905642 36-37 0.1787088049923786 38-39 0.19190225063724076 40-41 0.180460852100147 42-43 0.181681975841925 44-45 0.1857435395917518 46-47 0.2073786667558618 48-49 0.22349219091367134 50-51 0.23984463058617655 52-53 0.25972771064251815 54-55 0.2523744220235507 56-57 0.22269580586468568 58-59 0.21659018715579573 60-61 0.19535325251617855 62-63 0.18489406220616708 64-65 0.17693021171631065 66-67 0.17294828647138244 68-69 0.17841679714108385 70-71 0.18308892276179964 72-73 0.21714765669008568 74-75 0.22999600214705407 76-77 0.2464546264927574 78-79 0.23525214347035936 80-81 0.24016318460577082 82-83 0.2365529057170359 84-85 0.22158086679610578 86-87 0.21194460770337947 88-89 0.21438685518693545 90-91 0.21619199463130293 92-93 0.22266925969638618 94-95 0.22816431653438712 96-97 8.711125127487973 98-99 0.2841236393097784 100-101 0.296016322707964 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1883511.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.57211461085872 #Duplication Level Percentage of deduplicated Percentage of total 1 76.22164014490843 40.07132801525756 2 12.176307876909675 12.802685064839944 3 4.588035956986179 7.236082565082548 4 2.274670752791125 4.783370060708132 5 1.2644564728038887 3.32375753043441 6 0.7978341243891266 2.5166296216703565 7 0.5314948712241935 1.9559266499557324 8 0.377662831811989 1.5883626942625078 9 0.291593209284821 1.379670445644275 >10 1.3556950101078387 12.53080063443845 >50 0.07179287832201325 2.5873074987486055 >100 0.04147369083593685 4.05828468272326 >500 0.004239850205856675 1.570505920466574 >1k 0.0027920964770275667 2.350920365505956 >5k 2.0682196126130126E-4 0.6747282255230771 >10k+ 1.0341098063065063E-4 0.569640024738603 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10478 0.5563015028847721 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7221 0.3833797625816892 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5190 0.27554922694903294 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2418 0.12837726989648587 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2391 0.1269437768083117 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2161 0.11473253939053184 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2151 0.1142016160245414 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2081 0.11048515246260839 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1976 0.1049104571197089 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.185540195942577E-4 2 0.0 0.0 0.0 0.0 3.185540195942577E-4 3 0.0 0.0 0.0 0.0 3.185540195942577E-4 4 0.0 0.0 0.0 0.0 3.185540195942577E-4 5 0.0 0.0 0.0 0.0 3.716463561933007E-4 6 0.0 0.0 0.0 0.0 4.7783102939138664E-4 7 0.0 0.0 0.0 0.0 4.7783102939138664E-4 8 0.0 0.0 0.0 0.0 5.309233659904296E-4 9 0.0 0.0 0.0 0.0 5.309233659904296E-4 10-11 0.0 0.0 0.0 2.6546168299521478E-5 5.840157025894725E-4 12-13 0.0 0.0 0.0 5.3092336599042956E-5 0.00143349308817416 14-15 0.0 0.0 0.0 7.963850489856443E-5 0.0018051394443674607 16-17 0.0 0.0 0.0 1.0618467319808591E-4 0.0021767858005607614 18-19 5.3092336599042956E-5 0.0 0.0 1.0618467319808591E-4 0.002229878137159804 20-21 5.3092336599042956E-5 0.0 0.0 1.327308414976074E-4 0.0027077091665511912 22-23 5.3092336599042956E-5 0.0 0.0 4.512848610918651E-4 0.0028404400080487982 24-25 5.3092336599042956E-5 0.0 0.0 0.001114939068579902 0.0030262631861454484 26-27 5.3092336599042956E-5 0.0 0.0 0.003105901691044013 0.0035837327204353998 28-29 5.3092336599042956E-5 0.0 0.0 0.010538828814910027 0.0037164635619330068 30-31 5.3092336599042956E-5 0.0 0.0 0.03464274963087553 0.003875740571730136 32-33 5.3092336599042956E-5 0.0 0.0 0.07666533404901803 0.004194294591324393 34-35 1.0618467319808591E-4 0.0 0.0 0.13527927365436146 0.00467212562071578 36-37 1.0618467319808591E-4 0.0 0.0 0.23533178197525792 0.00467212562071578 38-39 1.0618467319808591E-4 0.0 0.0 0.39243200597182604 0.004751764125614345 40-41 1.0618467319808591E-4 0.0 0.0 0.5679818169365616 0.004778310293913866 42-43 1.0618467319808591E-4 0.0 0.0 0.7412486574275383 0.004884494967111953 44-45 1.0618467319808591E-4 0.0 0.0 0.9454152378191579 0.0050968643135081235 46-47 1.0618467319808591E-4 0.0 0.0 1.18605625345432 0.005362325996503339 48-49 1.0618467319808591E-4 0.0 0.0 1.4463679797994278 0.005760518520996161 50-51 1.0618467319808591E-4 0.0 0.0 1.7435257877442711 0.006025980203991376 52-53 1.0618467319808591E-4 0.0 0.0 2.0166593133780477 0.00647726506508324 54-55 1.0618467319808591E-4 0.0 0.0 2.2899786621899207 0.006689634411479413 56-57 1.0618467319808591E-4 0.0 0.0 2.600515738957723 0.006742726748078455 58-59 1.0618467319808591E-4 0.0 0.0 2.9413419937552794 0.006769272916377977 60-61 1.5927700979712886E-4 0.0 0.0 3.3166251750056146 0.006902003757875585 62-63 1.5927700979712886E-4 0.0 0.0 3.6779450717304014 0.007220557777469842 64-65 1.5927700979712886E-4 0.0 0.0 4.0247176682270505 0.007326742450667928 66-67 1.8582317809665033E-4 0.0 0.0 4.426334648430511 0.0074329271238660136 68-69 2.1236934639617182E-4 0.0 0.0 4.862647470601446 0.007512565628764578 70-71 2.1236934639617182E-4 0.0 0.0 5.3239136909739315 0.007618750301962664 72-73 2.1236934639617182E-4 0.0 0.0 5.79887773418897 0.00809658133135405 74-75 2.1236934639617182E-4 0.0 0.0 6.244986092462428 0.008362043014349264 76-77 2.1236934639617182E-4 0.0 0.0 6.7113226309801215 0.008547866192445916 78-79 2.1236934639617182E-4 0.0 0.0 7.237388048171739 0.008627504697344482 80-81 2.1236934639617182E-4 0.0 0.0 7.783867468785688 0.008733689370542566 82-83 2.1236934639617182E-4 0.0 0.0 8.350973262168365 0.009184974231634432 84-85 2.1236934639617182E-4 5.3092336599042956E-5 0.0 8.896205012872237 0.009184974231634432 86-87 2.1236934639617182E-4 5.3092336599042956E-5 0.0 9.360736411945563 0.009370797409731083 88-89 2.1236934639617182E-4 5.3092336599042956E-5 0.0 9.814941351550376 0.009609712924426776 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4595 0.0 56.51257 1 GTATCAA 9635 0.0 47.311157 1 TATCAAC 12470 0.0 36.253376 2 ATCAACG 12870 0.0 35.275642 3 TCAACGC 13065 0.0 34.893177 4 CAACGCA 13265 0.0 34.050602 5 AACGCAG 13760 0.0 32.997513 6 ACGCAGA 15445 0.0 29.123419 7 CGCAGAG 15530 0.0 28.903425 8 GCAGAGT 15575 0.0 27.09797 9 AGGTAAG 955 0.0 27.09261 2 TGGTATC 2090 0.0 26.785034 2 GTGGTAT 2170 0.0 26.447926 1 TACATGG 10690 0.0 26.35971 2 GTACATG 10740 0.0 26.193192 1 ACATGGG 11265 0.0 24.139198 3 GAGTACT 9310 0.0 23.120459 12-13 CATGGGA 6150 0.0 22.872952 4 GTAAGGT 1240 0.0 22.764374 4 TAAGGTG 1280 0.0 22.422327 5 >>END_MODULE