FastQCFastQC Report
Fri 27 May 2016
SRR939892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883511
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112010.5946872622458801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91060.48345881707088517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64120.34042806227306344No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28210.1497734815459002No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26760.14207509273903896No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG25680.13634112038634233No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25410.13490762729816816No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25300.13432361159557868No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23720.1259350224129299No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20190.10719342759346773No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA19670.10443262609031749No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47700.061.9711881
GTATCAA89350.043.704431
AGGTAAG11650.032.578162
TATCAAC121600.032.0701332
ATCAACG124100.031.380853
TCAACGC126450.030.7976554
TAAGGTG13800.030.5917435
CAACGCA128900.030.2122865
GTGGTAT18900.029.6237721
AACGCAG134150.029.0299176
TGGTATC19050.028.1414182
GGTAAGG14200.028.059783
AAGGTAA13200.027.6781441
TAAGGTA6400.027.4230084
GTAAGGT14700.026.7826844
ACGCAGA151950.025.566817
CGCAGAG154400.025.1303968
GCAGAGT163850.023.275719
GTACATG104450.022.8950841
AAGGTGT19400.022.7391686